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| 1 | Structures of EV71 RNA-dependent RNA polymerase in complex with substrate and analogue provide a drug target against the hand-foot-and-mouth disease pandemic in China显示文摘Enterovirus 71(EV71),one of the major causative agents for hand-foot-and-mouth disease(HFMD),has caused more than 100 deaths among Chinese children since March 2008.The EV71 genome encodes an RNAdependent RNA polymerase(RdRp),denoted 3D^(pol),which is central for viral genome replication and is a key target for the discovery of specific antiviral therapeutics.Here we report the crystal structures of EV71 RdRp(3D^(pol))and in complex with substrate guanosine-5'-triphosphate and analog 5-bromouridine-5'-triphosphate best to 2.4Åresolution.The structure of EV71 RdRp(3D^(pol))has a wider open thumb domain compared with the most closely related crystal structure of poliovirus RdRp.And the EV71 RdRp(3D^(pol))complex with GTP or Br-UTP bounded shows two distinct movements of the polymerase by substrate or analogue binding.The model of the complex with the template:primer derived by superimposition with foot-and-mouth disease virus(FMDV)3D/RNA complex reveals the likely recognition and binding of template:primer RNA by the polymerase.These results together provide a molecular basis for EV71 RNA replication and reveal a potential target for anti-EV71 drug discovery. | Yang Wu Zhiyong Lou Yi Miao Yue Yu Hui Dong Wei Peng Mark Bartlam Xuemei Li Zihe Rao | 2010 | Protein & Cell2010，1，5： | 30 |
| 2 | Different aspects of cognitive function in adult patients with moyamoya disease and its clinical subtypes显示文摘Objective Although a few reports suggested that cognitive function impairment could be found in adult patients with moyamoya disease(MMD),there were still many aspects that are unclear.The aim of our study was to assess the cognitive function of adult patients with MMD and its clinical subtypes.Methods 49 patients with MMD and 23 healthy controls were asked to take cognitive function tests.Cognitive function tests included IQ,prospective memory(PM),immediate memory(IM),verbal fluency(VF),visual breadth,attention,retrospective memory(RM),Stroop test,Wisconsin Card Sorting Test,Trail-Making Test Part A(TMT-A)and Continuous Performance Test(CPT).Independent t-analysis,one-way analysis of variance and Pearson correlation were used to seek for differences between subgroups and the correlation between cognitive variables.results Compared with healthy controls,adult patients with MMD had a comprehensive cognitive impairment,including IQ,PM,VF,attention,RM,Stroop,CPT and TMT-A,with more serious impairment in PM and attention.PM and RM were separated,indicating that they were independent of each other.Pattern of attention was significantly different from healthy controls.Female patients were better than male patients,where significant differences in PM,IM,Stroop and WCST could be found.The haemorrhagic patients exhibited poorer in the dimension of PM and RM than the ischaemic.The headache subtype exhibited poorer than healthy controls.PM,RM,attention and executive function were moderately correlated with each other.Conclusions Adult patients with MMD had a wide range of cognitive impairment with more serious impairment in memory and attention.Differences in cognitive function existed between the different subtypes of adult MMD. | Zhiyong Shi Yu-Jie Wen Zheng Huang Le-Bao Yu Dong Zhang | 2020 | Stroke & Vascular Neurology2020，5，1： | 12 |
| 3 | Wheat powdery mildew resistance gene Pm64 derived from wild emmer (Triticum turgidum var.dicoccoides) is tightly linked in repulsion with stripe rust resistance gene Yr5显示文摘Stripe rust and powdery mildew are both devastating diseases for durum and common wheat.Pyramiding of genes conferring resistance to one or more diseases in a single cultivar is an important breeding approach to provide broader spectra of resistances in wheat improvement. A new powdery mildew resistance gene originating from wild emmer(Triticum turgidum var.dicoccoides) backcrossed into common wheat(T. aestivum) line WE35 was identified. It conferred an intermediate level of resistance to Blumeria graminis f. sp. tritici isolate E09 at the seedling stage and a high level of resistance at the adult plant stage. Genetic analysis showed that the powdery mildew resistance in WE35 was controlled by a dominant gene designated Pm64. Bulked segregant analysis(BSA) and molecular mapping indicated that Pm64 was located in chromosome bin 2 BL4-0.50–0.89. Polymorphic markers were developed from the corresponding genomic regions of Chinese Spring wheat and wild emmer accession Zavitan to delimit Pm64 to a 0.55 cM genetic interval between markers WGGBH1364 and WGGBH612, corresponding to a 15 Mb genomic region on Chinese Spring and Zavitan 2 BL, respectively. The genetic linkage map of Pm64 is critical for fine mapping and cloning. Pm64 was completely linked in repulsion with stripe rust resistance gene Yr5. Analysis of a larger segregating population might identify a recombinant line with both genes as a valuable resource in breeding for resistance to powdery mildew and stripe rust. | Deyun Zhang Keyu Zhu Lingli Dong Yong Liang Genqiao Li Tilin Fang Guanghao Guo Qiuhong Wu Jingzhong Xie Yongxing Chen Ping Lu Miaomiao Li Huaizhi Zhang Zhenzhong Wang Yan Zhang Qixin Sun Zhiyong Liu | 2019 | The Crop Journal2019，7，6： | 8 |
| 4 | Tianshanbeilu and the Isotopic Millet Road: reviewing the late Neolithic/Bronze Age radiation of human millet consumption from north China to Europe显示文摘The westward expansion of human millet consumption from north China has important implications for understanding early interactions between the East and West.However,few studies have focused on the Xinjiang Uyghur Autonomous Region,the vast geographical area directly linking the ancient cultures of the Eurasian Steppe and the Gansu Corridor of China.In this study,we present the largest isotopic investigation of Bronze Age China(n=110)on material from the key site of Tianshanbeilu,in eastern Xinjiang.The large range ofδ13C values(–17.6 to–7.2;–15.5±1.2)provides direct evidence of unique dietary diversity and consumption of significant C4 resources(millets).The highδ15N results(10.3 to 16.7;14.7±0.8)likely reflect sheep/goat and wild game consumption and the arid climate of the Taklamakan Desert.Radiocarbon dates from four individuals indicate Tianshanbeilu was in use between 1940 and1215 cal bc.The Tianshanbeilu results are then analysed with respect to 52 Bronze Age sites from across Eurasia,to investigate the spread and chronology of significant human millet consumption and human migration.This isotopic survey finds novel evidence that the second millennium bc was a dynamic period,with significant dietary interconnectivity occurring between north China,Central Asia and Siberia.Further,we argue that this‘Isotopic Millet Road’extended all the way to the Mediterranean and Central Europe,and conclude that these C4 dietary signatures of millet consumption reflect early links(migration and/or resource transfer)between the Bronze Age inhabitants of modern-day China and Europe. | Tingting Wang Dong Wei Xien Chang Zhiyong Yu Xinyu Zhang Changsui Wang Yaowu Hu Benjamin T.Fuller | 2019 | National Science Review2019，6，5： | 8 |
| 5 | Global warming projections using the human–earth system model BNU-HESM1.0显示文摘Future climate change is usually projected by coupled earth system models under specific emission scenarios designed by integrated assessment models(IAMs),and this offline approach means there is no interaction between the coupled earth system models and the IAMs.This paper introduces a new method to design possible future emission scenarios and corresponding climate change, in which a simple economic and climate damage component is added to the coupled earth system model of Beijing Normal University(BNU-ESM). With the growth of population and technological expertise and the declining emission-to-output ratio described in the Dynamic Integrated Climate-Economy model, the projected carbon emission is 13.7 Gt C, resulting in a 2.4 °C warming by the end of the twenty-first century(2080–2099) compared with1980–1999. This paper also suggests the importance of the land and ocean carbon cycle in determining the CO2 concentration in the atmosphere. It is hoped that in the near future the next generation of coupled earth system models that include both the natural system and the social dimension will be developed. | Shili Yang Wenjie Dong Jieming Chou Jinming Feng Zhigang Wei Yan Guo Xiaohang Wen Ting Wei Di Tian Xian Zhu Zhiyong Yang | 2016 | Science Bulletin2016，61，23： | 7 |
| 6 | The effect of 2,4-diamino-6-hydroxy-pyrimidine on PoStburn Staphylococcus aureus sepsis in rats显示文摘Objective;GTP-cyclohydrolase I (GTP-CHI) is the first and rate-limiting enzyme for the de novo bio-synthesis of biopterin.The present study was to observe the effect of 2,4-diamino-6-hydroxy-pyrimidine (DAHP),an inbibtor of GTP-CHI,on the development of postburn Staphylococcus aureus sepsis.Methods;56 male Wistarrats were randomly divided into four groups as follows;normal control group (n=10),scald control group(n=10),postburn sepsis group (n=20) and DAHP treatment group (n=16).In the scald control gro... | Li Hongyun Yao Yongming Shi ZhiGuo Dong Ning Yu Yan Lu Lianrong Sheng Zhiyong (C.Y.Sheng) Research Department of Burn Institute,304th Hospital of PLA,Beijing 100037 | 2002 | 感染.炎症.修复2002，3，1： | 6 |
| 7 | Insight into the Ebola virus nucleocapsid assembly mechanism: crystal structure of Ebola virus nucleoprotein core domain at 1.8 A resolution显示文摘 | Shishang Dong Peng Yang Guobang Li Baocheng Liu Wenming Wang Xiang Liu Boran Xia Cheng Yang Zhiyong Lou Yu Guo Zihe Rao | 2015 | Protein & Cell2015，6，5： | 6 |
| 8 | Classification and Facies Sequence Model of Subaqueous Debris Flows显示文摘Objective Debris flows are cohesive sediment gravity flows which occur in both subaerial and subaqueous settings.Compared to subaerial debris flows which have been well studied as a geological hazard,subaqueous debris flows showing complicated sediment composition and sedimentary processes were poorly understood.The main objective | XIAN Benzhong LIU Jianping DONG Yanlei LU Zhiyong HE Yanxin WANG Junhui | 2017 | Acta Geologica Sinica(English Edition)2017，91，2： | 6 |
| 9 | Significantly enhanced piezoelectric performance in Bi_(4)Ti_(3)O_(12)-based high-temperature piezoceramics via oxygen vacancy defects tailoring显示文摘Bismuth titanate (Bi_(4)Ti_(3)O_(12),BIT)piezoelectric materials have attracted increasing attention due to their high-temperature applications.However,it is quite challenging to simultaneously achieve outstanding piezoelectric properties and high Curie temperature in BIT-based systems.In this study,oxygen vacancy defects tailoring strategy was utilized to solve this problem,excellent piezoelectric coefficient(32.1 pC/N),and ultrahigh Curie temperature(659℃)are gotten in Bi_(4)Ti_(3)-x(Mn_(1/3)Nb_(2/3))xO_(12)(BTMN)ceramics,which are among the top values in the BIT-based ceramics.More importantly,the(Mn_(1/3)Nb_(2/3))(4+d)+complex-ion modified Bi_(4)Ti_(3)O_(12)-based ceramics are characterized with excellent piezoelectric stability up to 500℃(d33>30.0 pC/N at 500℃))and significantly reduced conductivity(only~10^(-7)U-1 cm^(-1)at 500℃).Moreover,enhanced ferroelectricity and good dielectric stability were also obtained.The better comprehensive properties can be ascribed to two aspects.First,the concentration of oxygen vacancy defects is obviously reduced,and their distribution is effectively controlled in BITMN ceramics.Second,the introduction of(Mn_(1/3)Nb_(2/3))^((4+δ)+)complex-ion gives rise to the antiphase boundaries and massive ferroelectric domain walls.This works not only reveal the high potential of BITMN ceramics for high-temperature piezoelectric applications but also deepen the understanding of the structure-properties relationship in BIT-based materials. | Xinchun Xie Zhiyong Zhou Ruihong Liang Xianlin Dong | 2021 | Journal of Materiomics2021，7，1： | 6 |
| 10 | Near-surface wind environment in the Yarlung Zangbo River basin,southern Tibetan Plateau显示文摘Aeolian processes have been studied extensively at low elevations,but have been relatively little studied at high elevations.Aeolian sediments are widely distributed in the Yarlung Zangbo River basin,southern Tibetan Plateau,which is characterized by low pressure and low temperature.Here,we comprehensively analyzed the wind regime using data since 1980 from 11 meteorological stations in the study area,and examined the interaction between the near-surface wind and aeolian environment.The wind environment exhibited significant spatial and temporal variation,and mean wind speed has generally decreased on both annual and seasonal bases since 1980,at an average of 0.181 m/(s•10a).This decrease resulted from the reduced contribution of maximum wind speed,and depended strongly on variations of the frequency of sand-driving winds.The drift potential and related parameters also showed obvious spatial and temporal variation,with similar driving forces for the wind environment.The strength of the wind regime affected the formation and development of the aeolian geomorphological pattern,but with variation caused by local topography and sediment sources.The drift potential and resultant drift direction were two key parameters,as they quantify the dynamic conditions and depositional orientation of the aeolian sediments.Wind affected the spatial variation in sediment grain size,but the source material and complex topographic effects on the near-surface wind were the underlying causes for the grain size distribution of aeolian sands.These results will support efforts to control aeolian desertification in the basin and improve our understanding of aeolian processes in high-elevation environments. | YANG Junhuai XIA Dunsheng WANG Shuyuan TIAN Weidong MA Xingyue CHEN Zixuan GAO Fuyuan LING Zhiyong DONG Zhibao | 2020 | Journal of Arid Land2020，12，6： | 5 |
| 11 | Ketamine induces tau hyperphosphorylation at serine 404 in the hippocampus of neonatal rats显示文摘Male Wistar 7-day-old rats were injected with 40 mg/kg ketamine intraperitoneally, followed by three additional injections of 20 mg/kg ketamine each upon restoration of the righting reflex. Neonatal rats injected with equivalent volumes of saline served as controls. Hippocampal samples were collected at 1, 7 or 14 days following administration. Electron microscopy showed that neuronal structure changed noticeably following ketamine treatment. Specifically, microtubular structure became irregular and disorganized. Quantitative real time-PCR revealed that phosphorylated tau mRNA was upregulated after ketamine. Western blot analysis demonstrated that phosphorylated tau levels at serine 396 initially decreased at 1 day after ketamine injection, and then gradually returned to control values. At 14 days after injection, levels of phosphorylated tau were higher in the ketamine group than in the control group. Tau protein phosphorylated at serine 404 significantly increased after ketamine injection, and then gradually decreased with time. However, the levels of tau protein at serine 404 were significantly greater in the ketamine group than in the control group until 14 days. The present results indicate that ketamine induces an increase of phosphorylated tau mRNA and excessive phosphorylation of tau protein at serine 404, causing disruption of microtubules in the neonatal rat hippocampus and potentially resulting in damage to hippocampal neurons. | Haiyan Jin Zhiyong Hu Mengjie Dong Yidong Wu Zhirui Zhu Lili Xu | 2013 | Neural Regeneration Research2013，8，17： | 5 |
| 12 | Haemodynamic analysis of adult patients with moyamoya disease: CT perfusion and DSA gradings显示文摘Object Moyamoya disease(MMD)is a common and chronic progressive stenotic-occlusive cerebrovascular disease in Eastern Asia.To evaluate the hemispheric haemodynamic status of adult patients with MMD,we explored the potential risk factors of hemispheric perfusion alterations with CT perfusion(CTP)and DSA.Methods We retrospectively reviewed 44 male and 44 female(176 hemispheres)adult patients with MMD who had both DSA and CTP.Data on cerebral blood perfusion(CBF),cerebral blood volume(CBV),mean transmit time(MTT),time to peak(TTP)of cerebral hemisphere and cerebellum were gathered and difference of relative haemodynamic parameters between different subgroups were assessed with independent sample t analysis,one-way analysis of variance and general linear regression analysis.Results Parameters in regional CBF(rCBF)of frontal,temporal lobe and basal ganglia in female was more superior than male.rCBF,regional MTT(rMTT)and regional TTP(rTTP)in adult MMD patients with haemorrhage were superior than the ischaemic.With the increase of age,significant difference could be seen in rCBF and rCBV of thalamus.However,with progress of arterial stenosis,significant difference could only be obsevrved in rCBV,rMTT and rTTP,whereas rCBF had no significant difference.For increase of moyamoya vessels,significant decrease of rCBF could be seen in temporal and parietal lobe.With the increase of compensatory artery numbers,no significant difference could be seen in rCBF parameters(p>0.05).Conclusions In adult MMD patients,age,gender and clinical type were potential risk factors for the change of cerebral perfusion.When arterial stenosis is worsened,moyamoya vessels could alter perfusion of temporal and parietal lobe,but not frontal lobe.Extracranial/intracranial compensatory arteries could maintain microcirculation stability in frontal lobe and basal ganglia,indicating that the protection from extracranial compensatory arteries,a theoretic base for surgery treatment if necessary. | Zhiyong Shi Guofeng Ma Dong Zhang | 2021 | Stroke & Vascular Neurology2021，6，1： | 5 |
| 13 | Robotic stereotactic irradiation and reirradiation for spinal metastases: safety and efficacy assessment显示文摘 | Wang Qi Song Yongchun Zhuang Hongqing Wang Xiaoguang Li Fengtong Dong Yang Wang Jingsheng Yuan Zhiyong | 2014 | Chinese Medical Journal2014，，2： | 4 |
| 14 | Chinese expert consensus on conversion therapy for hepatocellular carcinoma(2021 edition)显示文摘Recent advances in systemic and locoregional treatments for patients with unresectable or advanced hepatocellular carcinoma(HCC)have resulted in improved response rates.This has provided an opportunity for selected patients with initially unresectable HCC to achieve adequate tumor downstaging to undergo surgical resection,a‘conversion therapy’strategy.However,conversion therapy is a new approach to the treatment of HCC and its practice and treatment protocols are still being developed.Review the evidence for conversion therapy in HCC and develop consensus statements to guide clinical practice.Evidence review:Many research centers in China have accumulated significant experience implementing HCC conversion therapy.Preliminary findings and data have shown that conversion therapy represents an important strategy to maximize the survival of selected patients with intermediate stage to advanced HCC;however,there are still many urgent clinical and scientific challenges for this therapeutic strategy and its related fields.In order to summarize and learn from past experience and review current challenges,the Chinese Expert Consensus on Conversion Therapy for Hepatocellular Carcinoma(2021 Edition)was developed based on a review of preliminary experience and clinical data from Chinese and non-Chinese studies in this field and combined with recommendations for clinical practice.Sixteen consensus statements on the implementation of conversion therapy for HCC were developed.The statements generated in this review are based on a review of clinical evidence and real clinical experience and will help guide future progress in conversion therapy for patients with HCC. | Hui-Chuan Sun Jian Zhou Zheng Wang Xiufeng Liu Qing Xie Weidong Jia Ming Zhao Xinyu Bi Gong Li Xueli Bai Yuan Ji Li Xu Xiao-Dong Zhu Dousheng Bai Yajin Chen Yongjun Chen Chaoliu Dai Rongping Guo Wenzhi Guo Chunyi Hao Tao Huang Zhiyong Huang Deyu Li Gang Li Tao Li Xiangcheng Li Guangming Li Xiao Liang Jingfeng Liu Fubao Liu Shichun Lu Zheng Lu Weifu Lv Yilei Mao Guoliang Shao Yinghong Shi Tianqiang Song Guang Tan Yunqiang Tang Kaishan Tao Chidan Wan Guangyi Wang Lu Wang Shunxiang Wang Tianfu Wen Baocai Xing Bangde Xiang Sheng Yan Dinghua Yang Guowen Yin Tao Yin Zhenyu Yin Zhengping Yu Bixiang Zhang Jialin Zhang Shuijun Zhang Ti Zhang Yamin Zhang Yubao Zhang Aibin Zhang Haitao Zhao Ledu Zhou Wu Zhang Zhenyu Zhu Shukui Qin Feng Shen Xiujun Cai Gaojun Teng Jianqiang Cai Minshan Chen Qiang Li Lianxin Liu Weilin Wang Tingbo Liang Jiahong Dong Xiaoping Chen Xuehao Wang Shusen Zheng Jia Fan 无 | 2022 | Hepatobiliary Surgery and Nutrition2022，11，2： | 4 |
| 15 | NS1-binding protein radiosensitizes esophageal squamous cell carcinoma by transcriptionally suppressing c-Myc显示文摘Background:Cisplatin-based chemotherapy with concurrent radiotherapy is a standard treatment for advanced esophageal squamous cell carcinoma(ESCC).NS1-binding protein(NS1-BP),a member of the BTB-kelch protein fam-ily,has been shown to inhibit the proliferation of Hela cells by suppressing c-Myc.In the present study,we examined the potential function role of NS1-BP expression in ESCC,and particularly,the sensitivity of ESCC to radiotherapy.Methods:NS1-BP expression was examined using immunohistochemistry in two cohorts(n=98 for the training cohort;n=46 for independent validation cohort)of ESCC patients receiving cisplatin-based chemotherapy and con-current radiotherapy.Normal esophageal mucosal tissue blocks were used as a control.We also conducted a series of in vitro and in vivo experiments to examine the potential effects of over-expressing NS1-BP on ESCC cells,and particu-larly their sensitivity to ionizing irradiation.Results:In the training cohort,NS1-BP downregulation was observed in 59%(85/144)of the ESCC specimens.NS1-BP downregulation was associated with chemoradiotherapeutic resistance and shorter disease-specific survival(DSS)in both the training and validation cohorts.Over-expressing NS1-BP in cultured ESCC cells substantially increased the cellular response to irradiation both in vitro and in vivo.NS1-BP also significantly enhanced IR-induced apoptosis,and abrogated IR-induced G2/M cell-cycle arrest and ATM/Chk1 phosphorylation.Immunoprecipitation assays indicated that NS1-BP could interact with c-Myc promoter regions to inhibit its transcription.In ESCC tissues,c-Myc expression was inversely correlated with NS1-BP levels,and was associated with a shorter DSS.Conclusions:Our findings highlight the role and importance of NS1-BP in radiosensitivity of ESCC.Targeting the NS1-BP/c-Myc pathway may provide a novel therapeutic strategy for ESCC. | Yuwen Wang Jingjing Cheng Dan Xie Xiaofeng Ding Hailing Hou Xi Chen Puchun Er Furong Zhang Lujun Zhao Zhiyong Yuan Qingsong Pang Ping Wang Dong Qian | 2018 | Cancer Communications2018，38，1： | 4 |
| 16 | Subtyping of type A influenza by sequencing the variable regions of HA gene specifically amplified with RT-PCR显示文摘The outbreak of a novel influenza A(H1N1) virus across the globe poses a threat to human health.It is of paramount importance to develop a rapid,reliable and inexpensive diagnostic procedure.Based on the bioinformatic information from public database,primers specific for influenza A virus surface protein haemagglutinin(HA) of several subtypes(including H1,H2,H3,H5,H7 and H9) were designed.Primer-specific PCR products were subjected to sequencing for accurately distinguishing H1 and H3 subtypes from others.This sequencing-based detection method will not only be applied to rapid detection and simultaneous subtype identification of new influenza A virus H1N1,but also provide the strategies to monitor other new types ofinfluenza virus with explosive potential. | YAN An DING GuoHui ZHOU ZhenFeng DONG Hui ZHANG YaKun ZHU Lei HE YunGang ZHANG GuoQing LI YiXue SUN Bing HUANG Zhong LAN Ke JIN Li WANG HongYan WANG XiaoNing YANG Zhong ZHONG Yang DAI JianXin GUO YaJun WANG Hao CHE XiaoYan WU Fan YUAN ZhenGan ZHANG Xi CAO ZhiWei ZHOU XiaoNong ZHOU JiaHai MA ZhiYong TONG GuangZhi ZHAO GuoPing JIN WeiRong XIONG Hui | 2009 | Chinese Science Bulletin2009，54，13： | 3 |
| 17 | NS1结合蛋白通过转录抑制c-Myc增强食管鳞状细胞癌的放疗敏感性显示文摘背景与目的基于顺铂的化疗联合放疗是晚期食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)的标准疗法。NS1结合蛋白(NS1-binding protein,NS1-BP)是BTB-kelch蛋白家族中的一员,已被证明可通过抑制c-Myc进而抑制HeLa细胞增殖。在本研究中,我们检测NS1-BP表达在ESCC中的潜在作用,特别是其在ESCC放疗敏感性方面的作用。方法对于两组接受基于顺铂化疗且联合放疗的ESCC患者(研究组n=98;验证组n=46),采用免疫组化方法分别检测NS1-BP表达。使用正常食管黏膜组织块作为对照。我们也进行了一系列体外和体内实验来检测过表达NS1-BP对ESCC细胞各方面的潜在影响,尤其关注的是对电离辐射的敏感性方面的影响。结果在研究组中,59%(85/144)的ESCC样本观察到了NS1-BP下调。在研究组和验证组中,NS1-BP下调与放化疗抵抗和较短的疾病特异生存期(disease-specific survival,DSS)均具有相关性。体内和体外培养条件下过表达NS1-BP均能大幅度提高ECSS细胞对放疗的敏感性。NS1-BP也显著增强IR诱导的凋亡,阻断IR诱导的G2/M细胞周期阻滞和ATM/Chk1磷酸化。免疫沉淀反应显示NS1-BP可以与c-Myc启动子区相互作用从而抑制其转录。在ESCC组织中,c-Myc表达与NS1-BP水平负相关,与较短的疾病特异生存期相关。结论我们的研究结果显示了NS1-BP在ESCC放疗敏感性中的作用和重要性。以NS1-BP/c-Myc通路为靶标有望开发一种新的ESCC治疗策略。 | Yuwen Wang Jingjing Cheng Dan Xie Xiaofeng Ding Hailing Hou Xi Chen Puchun Er Furong Zhang Lujun Zhao Zhiyong Yuan Qingsong Pang Ping Wang Dong Qian | 2019 | 癌症2019，38，2： | 2 |
| 18 | Angiotensin I converting enzyme(ACE)inhibitory activity and antihypertensive effects of rice peptides显示文摘In this paper,the antihypertension effect of rice peptide(RP)was studied.With spontaneously hypertensive rats(SHR)and Wistar Kyoto(WKY)as the research objects,RP disposable gastric and long-term gastric irrigation experiments were carried out and systolic blood pressure(SBP)was measured.At the end of the long-term gastric irrigation experiment,the content of nitric oxide(NO),angiotensin-converting enzyme(ACE),angiotensin II(Ang II)and renin in the plasma and the activity of ACE were determined.The results showed that RP could reduce systolic pressure of SHR and had time-dose dependence while high-dose RP signifi cantly reduced systolic pressure by 24.6 and 17.2 mm Hg,respectively after a single and long-term gastric irrigation test.RP also could inhibit the activity of ACE and increase the release of NO.These results suggested that the decompression mechanism of RP is likely to be related to the regulation of the renin-angiotensin system(RAS)and NO. | Jingjing Dong Shen Wang Xiaoyao Yin Min Fang Zhiyong Gong Yongning Wu | 2022 | Food Science and Human Wellness2022，11，6： | 2 |
| 19 | Melatonin alleviates alcoholic liver disease via EGFR-BRG1-TERT axis regulation显示文摘Chronic alcohol consumption causes liver steatosis,cell death,and inflammation.Melatonin(MLT)is reported to alleviate alcoholic liver disease(ALD)-induced injury.However,its direct regulating targets in hepatocytes are not fully understood.In the current study,a cell-based screening model and a chronic ethanol-fed mice ALD model were used to test the protective mechanisms of MLT.MLT ameliorated ethanol-induced hepatocyte injury in both cell and animal models(optimal doses of 10μmol/L and 5 mg/kg,respectively),including lowered liver steatosis,cell death,and inflammation.RNA-seq analysis and loss-of-function studies in AML-12 cells revealed that telomerase reverse transcriptase(TERT)was a key downstream effector of MLT.Biophysical assay found that epidermal growth factor receptor(EGFR)on the hepatocyte surface was a direct binding and regulating target of MLT.Liver specific knock-down of Tert or Egfr in the ALD mice model impaired MLT-mediated liver protection,partly through the regulation of nuclear brahma-related gene-1(BRG1).Long-term administration(90 days)of MLT in healthy mice did not cause evident adverse effect.In conclusion,MLT is an efficacious and safe agent for ALD alleviation.Its direct regulating target in hepatocytes is EGFR and downstream BRG1-TERT axis.MLT might be used as a complimentary agent for alcoholics. | Zhaodi Che Yali Song Chengfang Xu Wei Li Zhiyong Dong Cunchuan Wang Yixing Ren Kwok-Fai So George L.Tipoe Fei Wang Jia Xiao | 2023 | Acta Pharmaceutica Sinica B2023，13，1： | 2 |
| 20 | Surgical treatment of hepato-pancreato-biliary disease in China:the Tongji experience显示文摘Hepato-pancreato-biliary(HPB) tumors are common in China. However, these tumors are often diagnosed at intermediate/advanced stages because of the lack of a systemic surveillance program in China. This situation creates many technical challenges for surgeons and increases the incidence of postoperative complications. Therefore, Dr. Xiao-Ping Chen has made many important technical improvements, such as Chen's hepatic portal occlusion method, the anterior approach for liver resection of large HCC tumors, the modified technique of Belghiti's liver-hanging maneuver, inserting biliary-enteric anastomosis technique, and invaginated pancreaticojujunostomy with transpancreatic U-sutures. These techniques are simple, practical, and easy to learn. Owing to these advantages, complicated surgical procedures can be simplified, and the curative effects are greatly improved. These improved techniques have been widely applied in China and will benefit many additional patients. In this review, we introduce our experience of surgically treating intermediate/advanced hepatocellular carcinoma(HCC), hilar cholangiocarcinoma(HC), and pancreatic carcinoma, mainly focusing on technical innovations established by Dr. Chen in HPB surgery. | binhao zhang wei dong hongping luo xuanru zhu lin chen changhai li wei zhang shuai xiang wanguang zhang zhiyong huang xiao-ping chen | 2016 | Science China(Life Sciences)2016，59，10： | 2 |
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