|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | HIF-1α-induced expression of m6A reader YTHDF1 drives hypoxia-induced autophagy and malignancy of hepatocellular carcinoma by promoting ATG2A and ATG14 translation显示文摘N6-methyladenosine(m6A),and its reader protein YTHDF1,play a pivotal role in human tumorigenesis by affecting nearly everystage of RNA metabolism.Autophagy activation is one of the ways by which cancer cells survive hypoxia.However,the possibleinvolvement of m6A modification of mRNA in hypoxia-induced autophagy was unexplored in human hepatocellular carcinoma(HCO).In this study,specific variations in YTHDF1 expression were detected in YTHDF1-overexpressing,knockout,and-knockdownHCC cells,HCC organoids,and HCC patient-derived xenograft(PDX)murine models.YTHDF1 expression and hypoxia inducedautophagy were significantly correlated in vitro;signifhcant overexpression of YTHDF1 in HCC tissues was associated with poorprognosis,Multivariate cox regression analysis identihed YTHDF1 expression as an independent prognostic factor in patients withHCC.Multiple HC models conhrmed that YTHDF1 deficiency inhibited HCC autophagy,growth,and metastasis.Luciferase reporterassays and chromatin immunoprecipitation demonstrated that HlIF-1a regulated YTHDF1 transcription by directly binding to itspromoter region under hypoxia.The results of methylated RNA immunoprecipitation sequencing,proteomics,and polysomeprofling indicated that YTHDF1 contibuted to the translation of autophagy-related genes ATG2A and ATG14 by binding to m6A-modifhed ATG2A and ATG14 mRNA,thus facilitating autophagy and autophagy-related malignancy of HCC.Taken together,HlE-1d-induced YTHDF1 expression was associated with hypoxia-induced autophagy and autophagy-related HCC progression via promoting translation of autophagy-related genes ATG2A and ATG14 in a m6A-dependent manner.Our fndings suggest thatYTHDF1 is a potential prognostic biomarker and therapeutic target for patients with HCC. | Qing Li Yong Ni Liren Zhang Runqiu Jiang Jing Xu Hong Yang Yuanchang Hu Jiannan Qiu Liyong Pu Jinhai Tang Xuehao Wang | 2021 | Signal Transduction and Targeted Therapy2021，6，3： | 24 |
| 2 | The mechanisms of sorafenib resistance in hepatocellular carcinoma:theoretical basis and therapeutic aspects显示文摘Sorafenib is a multikinase inhibitor capable of facilitating apoptosis,mitigating angiogenesis and suppressing tumor cell proliferation.In late-stage hepatocellular carcinoma(HCC),sorafenib is currently an effective first-line therapy.Unfortunately,the development of drug resistance to sorafenib is becoming increasingly common.This study aims to identify factors contributing to resistance and ways to mitigate resistance.Recent studies have shown that epigenetics,transport processes,regulated cell death,and the tumor microenvironment are involved in the development of sorafenib resistance in HCC and subsequent HCC progression.This study summarizes discoveries achieved recently in terms of the principles of sorafenib resistance and outlines approaches suitable for improving therapeutic outcomes for HCC patients. | Weiwei Tang Ziyi Chen Wenling Zhang Ye Cheng Betty Zhang Fan Wu Qian Wang Shouju Wang Dawei Rong F.P.Reiter E.N.De Toni Xuehao Wang | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 19 |
| 3 | Human CD39hi regulatory T cells present stronger stability and function under inflammatory conditions显示文摘 | Jian Gu Xuhao Ni Xiongxiong Pan Hao Lu Yunjie Lu Jie Zhao Song Guo Zheng Keli L Hippen Xuehao Wang Ling Lu | 2017 | Cellular & Molecular Immunology2017，14，6： | 6 |
| 4 | Chinese expert consensus on conversion therapy for hepatocellular carcinoma(2021 edition)显示文摘Recent advances in systemic and locoregional treatments for patients with unresectable or advanced hepatocellular carcinoma(HCC)have resulted in improved response rates.This has provided an opportunity for selected patients with initially unresectable HCC to achieve adequate tumor downstaging to undergo surgical resection,a‘conversion therapy’strategy.However,conversion therapy is a new approach to the treatment of HCC and its practice and treatment protocols are still being developed.Review the evidence for conversion therapy in HCC and develop consensus statements to guide clinical practice.Evidence review:Many research centers in China have accumulated significant experience implementing HCC conversion therapy.Preliminary findings and data have shown that conversion therapy represents an important strategy to maximize the survival of selected patients with intermediate stage to advanced HCC;however,there are still many urgent clinical and scientific challenges for this therapeutic strategy and its related fields.In order to summarize and learn from past experience and review current challenges,the Chinese Expert Consensus on Conversion Therapy for Hepatocellular Carcinoma(2021 Edition)was developed based on a review of preliminary experience and clinical data from Chinese and non-Chinese studies in this field and combined with recommendations for clinical practice.Sixteen consensus statements on the implementation of conversion therapy for HCC were developed.The statements generated in this review are based on a review of clinical evidence and real clinical experience and will help guide future progress in conversion therapy for patients with HCC. | Hui-Chuan Sun Jian Zhou Zheng Wang Xiufeng Liu Qing Xie Weidong Jia Ming Zhao Xinyu Bi Gong Li Xueli Bai Yuan Ji Li Xu Xiao-Dong Zhu Dousheng Bai Yajin Chen Yongjun Chen Chaoliu Dai Rongping Guo Wenzhi Guo Chunyi Hao Tao Huang Zhiyong Huang Deyu Li Gang Li Tao Li Xiangcheng Li Guangming Li Xiao Liang Jingfeng Liu Fubao Liu Shichun Lu Zheng Lu Weifu Lv Yilei Mao Guoliang Shao Yinghong Shi Tianqiang Song Guang Tan Yunqiang Tang Kaishan Tao Chidan Wan Guangyi Wang Lu Wang Shunxiang Wang Tianfu Wen Baocai Xing Bangde Xiang Sheng Yan Dinghua Yang Guowen Yin Tao Yin Zhenyu Yin Zhengping Yu Bixiang Zhang Jialin Zhang Shuijun Zhang Ti Zhang Yamin Zhang Yubao Zhang Aibin Zhang Haitao Zhao Ledu Zhou Wu Zhang Zhenyu Zhu Shukui Qin Feng Shen Xiujun Cai Gaojun Teng Jianqiang Cai Minshan Chen Qiang Li Lianxin Liu Weilin Wang Tingbo Liang Jiahong Dong Xiaoping Chen Xuehao Wang Shusen Zheng Jia Fan 无 | 2022 | Hepatobiliary Surgery and Nutrition2022，11，2： | 4 |
| 5 | Laser-based directed energy deposition of novel Sc/Zr-modified Al-Mg alloys:columnar-to-equiaxed transition and aging hardening behavior显示文摘The control of grain morphology is important in laser additive manufacturing(LAM),as grain morphology further affects the hot cracking resistance,anisotropy,and strength–ductility synergy of materials.To develop a solidification-control solution and achieve columnar-to-equiaxed transition(CET)in Al-based alloys during LAM,Sc-and-Zr-modified Al-Mg alloys were processed via directed energy deposition(DED).CET was achieved by introducing high potent primary Al_(3)(Sc,Zr)nucleation sites ahead of the solidification interface.Furthermore,the relationship between the solidification control parameters and precipitation behavior of primary Al_(3)(Sc,Zr)nucleation sites was established using the time-dependent nucleation theory.Then,the CET was studied according to the Hunt criterion.The results indicated that coarse columnar grain structure was still obtained at the inner region of the molten pool at low Sc/Zr contents owing to the effective suppression of the precipitation of the primary Al_(3)(Sc,Zr)nucleation sites via rapid solidification during DED.In addition,the relatively low melt temperature at the fusion boundary unavoidably promoted the precipitation of primary Al_(3)(Sc,Zr)nucleation sites,which resulted in a fine equiaxed grains band at the edge of the molten pool.As the Sc/Zr content increased,the solidification cooling rate was not sufficient to suppress the precipitation of the primary Al_(3)(Sc,Zr)nucleation sites,and a fully equiaxed grain structure was obtained.Furthermore,the effect of the layer-by-layer manufacturing process on the subsequent precipitation strengthening of secondary Al_(3)(Sc,Zr)precipitates was discussed.Both the remelting and subsequent aging during thermal cycling should be considered to achieve greater precipitation strengthening. | Zihong Wang Xin Lin Yao Tang Nan Kang Xuehao Gao Shuoqing Shi Weidong Huang | 2021 | Journal of Materials Science & Technology2021，，10： | 3 |
| 6 | IL-17A Plays a Critical Role in the Pathogenesis of Liver Fibrosis through Hepatic Stellate Cell Activation显示文摘 | Zhongming Tan Xiaofeng Qian Runqiu Jiang Qianghui Liu Youjing Wang Chen Chen Xuehao Wang Bernhard Ryffel Beicheng Sun | 2013 | The Journal of Immunology2013，，4： | 2 |
| 7 | Insights into flavonoid biosynthesis during cucumber fruit peel coloration based on metabolite profiling and transcriptome analyses显示文摘The fruit peel color is a crucial trait of cucumber.To better understand the molecular mechanisms underlying cucumber peel coloration,we compared the UPLC-ESI-MS/MS-based flavonoid metabolomic and RNA sequencing-based transcriptomic profiling of the brown peeled cucumber line‘PW’at six developmental stages.A total of 210 flavonoid metabolites were identified.Of which,117 flavonoid metabolites were differentially accumulated.In this study,weighted gene co-expression network analysis combined with Kyoto Encyclopedia of Gene and Genomes enrichment analysis revealed key genes coding for seven enzymes and eight transcription factors(TFs)associated with flavonoid biosynthesis.Among them,the R2R3MYB CsaV3_4G001130 is the best candidate gene that is responsible for controlling mature fruit colors in cucumber.Sanger sequencing revealed one nonsynonymous SNP in the exon of CsaV3_4G001130 among the selected 11 cucumber lines,which introduced a premature stop codon,generating a truncated protein in pale yellow or creamy peeled fruits.Yeast two-hybrid assays showed a direct interaction of CsaV3_4G001130 with the bHLH TF CsaV3_1G002260 and the WD40 protein CsaV3_5G001800.However,the interactions were influenced by the nonsynonymous SNP we identified.Our finding revealed that the integrated transcriptome and metabolome analysis further demonstrated that the abundance of some pigmented flavonoids(especially anthocyanins and chalcones)contributed to the coloration of‘PW’fruits.These findings pave the way for elucidation of flavonoid biosynthesis and improvement of cucumber peel color in the future. | Shuying He Yi Ye Ying Yuan Mai Lv Meixing Wang Qiang Xu Xuewen Xu Xuehao Chen | 2023 | Horticultural Plant Journal2023，9，4： | 2 |
| 8 | All-trans retinoic acid preconditioning protects against liver ischemia/reperfusion injury by inhibiting the nuclear factor kappa B signaling pathway显示文摘 | Jianhua Rao Xiaofeng Qian Ping Wang Liyong Pu Yuan Zhai Xuehao Wang Feng Zhang Ling Lu | 2012 | Journal of Surgical Research2012，，： | 2 |
| 9 | Molecularly tagged genes and quantitative trait loci in cucumber with recommendations for QTL nomenclature显示文摘Cucumber,Cucumis sativus L.(2n=2x=14),is an important vegetable crop worldwide.It was the first specialty crop with a publicly available draft genome.Its relatively small,diploid genome,short life cycle,and selfcompatible mating system offers advantages for genetic studies.In recent years,significant progress has been made in molecular mapping,and identification of genes and QTL responsible for key phenotypic traits,but a systematic review of the work is lacking.Here,we conducted an extensive literature review on mutants,genes and QTL that have been molecularly mapped or characterized in cucumber.We documented 81 simply inherited trait genes or major-effect QTL that have been cloned or fine mapped.For each gene,detailed information was compiled including chromosome locations,allelic variants and associated polymorphisms,predicted functions,and diagnostic markers that could be used for marker-assisted selection in cucumber breeding.We also documented 322 QTL for 42 quantitative traits,including 109 for disease resistances against seven pathogens.By alignment of these QTL on the latest version of cucumber draft genomes,consensus QTL across multiple studies were inferred,which provided insights into heritable correlations among different traits.Through collaborative efforts among public and private cucumber researchers,we identified 130 quantitative traits and developed a set of recommendations for QTL nomenclature in cucumber.This is the first attempt to systematically summarize,analyze and inventory cucumber mutants,cloned or mapped genes and QTL,which should be a useful resource for the cucurbit research community. | Yuhui Wang Kailiang Bo Xingfang Gu Junsong Pan Yuhong Li Jinfeng Chen Changlong Wen Zhonghai Ren Huazhong Ren Xuehao Chen Rebecca Grumet Yiqun Weng | 2020 | Horticulture Research2020，7，1： | 2 |
| 10 | Diagnosis and treatment of biliary sludge,cast and stone following liver transplantation显示文摘Objective:To investigate etiological facts and treatment of biliary sludge,cast and stone following orthotopic liver transplantation(OLT).Methods:A review was made using data collected from 81 cases with OLTs performed in our center from February 2003 to January 2004,and confirmed by retrospective study.Etiological factors of biliary sludge,cast and stone following OLT were analyzed,and treatment of biliary sludge,cast and stone following OLT were discussed.Result:Nine cases of biliary sludge,cast and stone were diagnosed and the incidence rate was 11.1%.Of these,five were biliary sludge and cast,2 were bile stone and 2 were necrotic debris.Two cases with hepatic artery embolism received retransplantation and survived.The other one with hepatic artery embolism was ameliorated with nasobiliary drainage by ERCP.Two cases with biliary sludge and cast were resolved by non-operative treatment.Four cases were reoperated,2 resolved and 2 cases died.Conclusion:Biliary injury and ischemia reperfusion injury,reject reaction,infection and changes of bile kinetics are the important factors causing biliary sludge,cast and stone following OLT.Shortening the time of cold and heat ischemia reperfusion injury of liver,reducing the injury of the blood supply of donor bile duct,actively preventing and early treating of infection and rejection reaction might reduce the incidence rate of biliary sludge,cast and stone following OLT. | Lianbao Kong Xuehao Wang Fen Zhang | 2006 | Journal of Nanjing Medical University2006，20，4： | 1 |
| 11 | Ozonation as an efficient pretreatment method to alleviate reverse osmosis membrane fouling caused by complexes of humic acid and calcium ion显示文摘Humic acid has been considered as one of the most significant sources in feed water causing organic fouling of reverse osmosis (RO) membranes, but the relationship between the fouling behavior of humic acid and the change of its molecular structure has not been well developed yet. In this study, the RO membrane fouling behavior of humic acid was studied systematically with ozonation as a pretreatment method to control RO membrane fouling. Furthermore, the effect of ozone on the structure ofhumic acid was also explored to reveal the mechanisms. Humic acid alone (10-90 mg/L, in deionized water) was found not to cause obvious RO membrane fouling in 45-h operation. However, the presence of Ca^2+ aggravated significantly the RO membrane fouling caused by humic acid, with significant flux reduction and denser fouling layer on RO membrane, as it was observed by scanning electron microscope (SEM) and atomic force microscope (AFM). However, after the pretreatment by ozone, the influence of Ca^2+ was almost eliminated. Further analysis revealed that the addition of Ca^2+ increased the particle size of humic acid solution significantly, while ozonation reduced the SUVA254, particle size and molecular weight of the complexes of humic acid and Ca^2+(HA-Ca^2+ complexes). According to these results and literature, the bridge effect of Ca^2+ aggregating humic acid molecules and the cleavage effect of ozone breaking HA-Ca^2+ complexes were summarized. The change of the structure of humic acid under the effect of Ca^2+ and ozone is closely related to the change of its membrane fouling behavior. | Xuehao Zhao Yinhu Wu Xue Zhang Xin Tong Tong Yu Yunhong Wang Nozomu Ikuno Kazuki Ishii Hongying Hu | 2019 | Frontiers of Environmental Science & Engineering2019，13，4： | 1 |
| 12 | Cucumber glossy fruit 1 (CsGLF1) encodes the zinc finger protein 6 that regulates fruit glossiness by enhancing cuticular wax biosynthesis显示文摘Cucumber glossiness is an important visual quality trait that affects consumer choice.Accumulating evidence suggests that glossy trait is associated with cuticular wax accumulation.However,the molecular genetic mechanism controlling cucumber glossiness remains largely unknown.Here,we report the map-based cloning and functional characterization of CsGLF1,a locus that determines the glossy trait in cucumber.CsGLF1 encodes a homolog of the Cys_(2)His_(2)-like fold group(C2H2)-type zinc finger protein 6(ZFP6)and its deletion leads to glossier pericarp and decreased cuticular wax accumulation.Consistently,transcriptomic analysis demonstrated that a group of wax biosynthetic genes were downregulated when CsZFP6 was absent.Further,transient expression assay revealed that CsZFP6 acted as a transcription activator of cuticular wax biosynthetic genes.Taken together,our findings demonstrated a novel regulator of fruit glossiness,which will provide new insights into regulatory mechanism of fruit glossiness in cucumber. | Yan Yang Congxi Cai Yipeng Wang Yanran Wang Haolun Ju Xuehao Chen | 2023 | Horticulture Research2023，10，1： | 1 |
| 13 | Comparative proteomic analysis of cucumber powdery mildew resistance between a singlesegment substitution line and its recurrent parent显示文摘Powdery mildew(PM)is considered a major cause of yield losses and reduced quality in cucumber worldwide,but the molecular basis of PM resistance remains poorly understood.A segment substitution line,namely,SSL508-28,was developed with dominant PM resistance in the genetic background of PM-susceptible cucumber inbred line D8.The substituted segment contains 860 genes.An iTRAQ-based comparative proteomic technology was used to map the proteomes of PM-inoculated and untreated(control)D8 and SSL508-28.The number of differentially regulated proteins(DRPs)in SSL508-28 was almost three times higher than that in D8.Fourteen DRPs were located in the substituted segment interval.Comparative gene expression analysis revealed that nodulin-related protein 1(NRP1)may be a good candidate for PM resistance.Gene Ontology enrichment analysis showed that DRPs functioning in tetrapyrrole biosynthetic process,sulfur metabolic process and cell redox homeostasis were specifically enriched in the resistant line SSL508-28.DRPs categorized in the KEGG term photosynthesis increased in both lines upon PM infection,suggesting that the strategies used by cucumber may be different from those used by other crops to react to PM attacks at the initial stage.The measurement of hydrogen peroxide and superoxide anion production and net photosynthetic rate were consistent with the changes in protein abundance,suggesting that the proteomic results were reliable.There was a poor correlation between DRPs measured by iTRAQ and the corresponding gene expression changes measured by RNA-seq with the same experimental design.Taken together,these findings improve the understanding of the molecular mechanisms underlying the response of cucumber to PM infection. | Xuewen Xu Xueli Liu Yali Yan Wei Wang Kiros Gebretsadik Xiaohua Qi Qiang Xu Xuehao Chen | 2019 | Horticulture Research2019，6，1： | 1 |
| 14 | Production of lipase by repeated batch fermentation with immobilized Rhizop us arrhizus显示文摘 | Yang Xuehao Wang Bingwu Cui Fengnan | 2005 | Process Biochemistry2005，40，： | 1 |
| 15 | Production of lipase by repeated batch fermentation with immobilized Rhizopus arrhizus显示文摘 | Yang Xuehao Wang Bingwu Cui Fengnan | 2005 | Process Biochemistry2005，40，： | 1 |
| 16 | IL-17A Plays a Critical Role in the Pathogenesis of Liver Fibrosis through Hepatic Stellate Cell Activation显示文摘 | Zhongming Tan Xiaofeng Qian Runqiu Jiang Qianghui Liu Youjing Wang Chen Chen Xuehao Wang Bernhard Ryffel Beicheng Sun | 2013 | The Journal of Immunology2013，，4： | 1 |
| 17 | Effect of Cu content on microstructure and mechanical properties of in-situ β phases reinforced Ti/Zr-based bulk metallic glass matrix composite by selective laser melting(SLM)显示文摘In this study,non-toxic in-situβphases of reinforced Ti/Zr-based bulk metallic glass matrix composites(BMGCs)of(Ti_(0.65)Zr_(0.35))100-xCux(x=5,10,15 at.%)are fabricated via selective laser melting.The effect of Cu content on phase formation,microstructure,and mechanical properties is investigated.The average volume fraction and width of theβphase decreases with increasing Cu content,while a more amorphous phase and the(Ti,Zr)_(2)Cu phase forms.In the center zone of the molten pool,theβphase grows in the direction of the temperature gradient,and the amorphous phase distributes among theβphases.This occurs using:sphere morphology(for x=5),a more continuous elongated sphere and network morphology(for x=10),and network morphology(for x=15),respectively.In the edge zone of the molten pool,due to the smaller cooling rate and the existence of a partially molten zone,theβphase becomes coarser,and an amorphous phase forms for more continuous networks.Furthermore,the hardness improves significantly with increasing Cu content.No crack is found for x=5.Although the average volume fraction of theβphase for x=5 is about 90%,the compression yield strength is 1386±64 MPa,reaching to an average level of conventionally fabricated counterparts,due to finer microstructure,and twinning and martensitic transformation of theβphase. | Xuehao Gao Xin Lin Qiaodan Yan Zihong Wang Xiaobin Yu Yinghui Zhou Yunlong Hu Weidong Huang | 2021 | Journal of Materials Science & Technology2021，，8： | 1 |
| 18 | Surgical Treatment of Recurrent Hepatocellular Carcinoma (A report of 112 cases)显示文摘Objective The aim of this study was to evaluate the long term results of treatment and prognositic factors in patients with recurrent hepatocellular carcinoma after curative resection of hepatocellular carcinoma. Methods 112 patients of recurrent hepatocellular carcinoma who underwent surgical treatment were studied. Survival results after recurrence and from first hepatectomy were analyzed, and prognostic factors were determined by analyzing the clinicopathological variables.Results The mean survival of 112 patients was 26 months (4 to 76 months). 30 patients with tumor free state were still living. 1 year, 3 year and 5 year survival rates were 81.0%, 43.3% and 32 0%, respectively, and the 58 patients with hepatic resection were 87%, 59% and 38%, respectively. Among the 9 patients with secondary re resection, 6 had lived for over 3 years, and two for over 5 years. There were no operative death in this series, and few complications were found.Conclusion Early detection of recurrence depends on AFP measurements and ultrasonography follow up monitoring after resection. Re resection for recurrent hepatocellular carcinoma has been proved to be the most effective treatment modality. Aggressive treatment with a multimodality strategy is an option to improve long term survival in some patients with unresectable recurrent hepatocellular carcinoma.\; | Li Xiangcheng(李相成) Wang Xuehao(王学浩) Du Jinghui(杜竞辉) Department of General Surgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, P.R.China | 2000 | Journal of Nanjing Medical University2000，14，1： | 0 |
| 19 | Living Donor Liver Transplantation for End-stage Liver Disease显示文摘 | Xuehao Wang Feng Zhang Xiangcheng Li Lianbao Kong Beicheng Sun Guoqiang Li Feng Cheng Ling Lv | 2008 | 器官移植内科学杂志2008，3，3： | 0 |
| 20 | Low-cost biodegradable lead sequestration film for perovskite solar cells显示文摘Despite the high efficiency that has been achieved for the perovskite solar cells(PSCs),the hazardous lead leakage from the perovskite absorber layer is one of the crucial barriers still hindering its penetration into the commercial market for a large-scale installation.Herein,we report a novel low-cost and biodegradable lead sequestration layer with high compatibility for up-scalable encapsulation of PSCs.Through a precisely designed cross-linking reaction of chemical agents,the as-made biodegradable chitosan composite film shows enhanced mechanical strength,chemical stability,and lead adsorption capacity.The designed encapsulation strategy reduces over 99.99% lead leakage to <2 ppb under varied simulations of weather conditions(hail,rain,or flood),which meet the safe level of drinking water set by the US Environmental Protection Agency(EPA).Moreover,the PSC efficiency is improved from 21.91% to22.82% due to the improved light absorption from the printed biodegradable lead absorption film.Finally,we present a prototype process of accumulation and recycling of lead compounds in PSCs derbies via the biodegradation process.Based on the low-cost biodegradable lead sequestration film,this environmental-friendly encapsulation strategy could address the lead leakage issue for further commercialization of PSCs. | Yiming Xiong Haoyu Cai Wang Yue Wenjian Shen Xuehao Zhu Juan Zhao Fuzhi Huang Yi-Bing Cheng Jie Zhong | 2023 | Journal of Energy Chemistry2023，，9： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员