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| 1 | An infectious disease of ducks caused by a newly emerged Tembusu virus strain in mainland China显示文摘 | Pixi Yan Youshu Zhao Xu Zhang Dawei Xu Xiaoguang Dai Qiaoyang Teng Liping Yan Jiewen Zhou Xiwen Ji Shumei Zhang Guangqing Liu Yanjun Zhou Yoshihiro Kawaoka Guangzhi Tong Zejun Li | 2011 | Virology2011，，1： | 5 |
| 2 | Host Interferon-Stimulated Gene 20 Inhibits Pseudorabies Virus Proliferation显示文摘Host interferon-stimulated gene 20(ISG20)exerts antiviral effects on viruses by degrading viral RNA or by enhancing IFN signaling.Here,we examined the role of ISG20 during pseudorabies virus(PRV)proliferation.We found that ISG20 modulates PRV replication by enhancing IFN signaling.Further,ISG20 expression was upregulated following PRV infection and poly(I:C)treatment.Ectopic expression of ISG20 inhibited PRV proliferation in PK15 cells,whereas knockdown of ISG20 promoted PRV proliferation.In addition,ISG20 expression upregulated IFN-βexpression and enhanced IFN downstream signaling during PRV infection.Notably,PRV UL24 suppressed the transcription of ISG20,thus antagonizing its antiviral effect.Further domain mapping analysis showed that the N terminus(amino acids 1-90)of UL24 was responsible for the inhibition of ISG20 transcription.Collectively,these findings characterize the role of ISG20 in suppressing PRV replication and increase the understanding of host-PRV interplay. | Xiaoyong Chen Dage Sun Sujie Dong Huanjie Zhai Ning Kong Hao Zheng Wu Tong Guoxin Li Tongling Shan Guangzhi Tong | 2021 | Virologica Sinica2021，36，5： | 4 |
| 3 | A Virulent PEDV Strain FJzz1 with Genomic Mutations and Deletions at the High Passage Level Was Attenuated in Piglets via Serial Passage In Vitro显示文摘Highly virulent porcine epidemic diarrhea virus(PEDV)strains re-emerged and circulated in China at the end of 2010,causing significant economic losses in the pork industry worldwide.To understand the genetic dynamics of PEDV during its passage in vitro,the PEDV G2 strain FJzzl was serially propagated in Vero cells for up to 200 passages.The susceptibility and adaptability of the FJzzl strain increased gradually as it was serially passaged in vitro.Sequence analysis revealed that amino acid(aa)changes were mainly concentrated in the S glycoprotein,which accounted for 72.22%-85.71%of all aa changes.A continuous aa deletion(^(55)I^(56)G^(57)E→^(55)K^(56)Δ^(57)Δ)occurred in the N-terminal domain of S1(Sl-NTD).To examine how the aa changes affected its virulence,FJzzl-F20 and FJzzl-F200 were selected to simultaneously evaluate their pathogenicity in suckling piglets.All the piglets in the FJzzl-F20-infected group showed typical diarrhea at 24 h postinfection,and the piglets died successively by 48 h postinfection.However,the clinical signs of the piglets in the FJzzl-F200-infected group were significantly weaker,and no deaths occurred.The FJzzl-F200-infected group also showed a lower level of fecal viral shedding and lower viral loads in the intestinal tissues,and no obvious histopathological lesions.TypeⅠandⅢinterferon were induced in the FJzzl-F200 infection group,together with pro-inflammatory cytokines,such as TNF-α,IL-1βand IL-8.These results indicate that the identified genetic changes may contribute to the attenuation of FJzzl strain,and the attenuated FJzzl-F200 may have the potential for developing PEDV live-attenuated vaccines. | Pengfei Chen Xiongwei Zhao Shuting Zhou Tianxing Zhou Xiangmei Tan Xia Wu Wu Tong Fei Gao Lingxue Yu Yifeng Jiang Hai Yu Zhibiao Yang Guangzhi Tong Yanjun Zhou | 2021 | Virologica Sinica2021，36，5： | 4 |
| 4 | Subtyping of type A influenza by sequencing the variable regions of HA gene specifically amplified with RT-PCR显示文摘The outbreak of a novel influenza A(H1N1) virus across the globe poses a threat to human health.It is of paramount importance to develop a rapid,reliable and inexpensive diagnostic procedure.Based on the bioinformatic information from public database,primers specific for influenza A virus surface protein haemagglutinin(HA) of several subtypes(including H1,H2,H3,H5,H7 and H9) were designed.Primer-specific PCR products were subjected to sequencing for accurately distinguishing H1 and H3 subtypes from others.This sequencing-based detection method will not only be applied to rapid detection and simultaneous subtype identification of new influenza A virus H1N1,but also provide the strategies to monitor other new types ofinfluenza virus with explosive potential. | YAN An DING GuoHui ZHOU ZhenFeng DONG Hui ZHANG YaKun ZHU Lei HE YunGang ZHANG GuoQing LI YiXue SUN Bing HUANG Zhong LAN Ke JIN Li WANG HongYan WANG XiaoNing YANG Zhong ZHONG Yang DAI JianXin GUO YaJun WANG Hao CHE XiaoYan WU Fan YUAN ZhenGan ZHANG Xi CAO ZhiWei ZHOU XiaoNong ZHOU JiaHai MA ZhiYong TONG GuangZhi ZHAO GuoPing JIN WeiRong XIONG Hui | 2009 | Chinese Science Bulletin2009，54，13： | 3 |
| 5 | The mutation network for the hemagglutinin gene from the novel influenza A (H1N1) virus显示文摘A mutation network for the hemagglutinin gene(HA) of the novel type A(H1N1) influenza virus was constructed.Sequence homology analysis indicated that one HA sequence type from the viruses mainly isolated from Mexico was likely the original type in this epidemic.Based on the 658A and 1408T mutations in HA,the viruses evolving into this epidemic were divided into three categories,the Mexico,the transitional and the New York type.The three groups of viruses presented distinctive clustering features in their geographic distributions. | HE YunGang DING GuoHui BIAN Chao HUANG Zhong LAN Ke SUN Bing WANG XueCai LI YiXue WANG HongYan WANG XiaoNing YANG Zhong ZHONG Yang JIN WeiRong XIONG Hui DAI JianXin GUO YaJun WANG Hao CHE XiaoYan WU Fan YUAN ZhenAn ZHANG Xi CAO ZhiWei ZHOU XiaoNong ZHOU JiaHai MA ZhiYong TONG GuangZhi ZHAO GuoPing JIN Li | 2009 | Chinese Science Bulletin2009，54，13： | 3 |
| 6 | Structure modeling and spatial epitope analysis for HA protein of the novel H1N1 influenza virus显示文摘In recent months,a novel influenza virus H1N1 broke out around the world.With bioinformatics technology,the 3D structure of HA protein was obtained,and the epitope residues were predicted with the method developed in our group for this novel flu virus.58 amino acids were identified as potential epitope residues,the majority of which clustered at the surface of the globular head of HA protein.Although it is located at the similar position,the epitope of HA protein for the novel H1N1 flu virus has obvious differences in the electrostatic potential compared to that of HA proteins from previous flu viruses. | WU Di XU TianLei SUN Jing DAI JianXin DING GuoHui HE YunGang ZHOU ZhengFeng XIONG Hui DONG Hui JIN WeiRong BIAN Chao JIN Li WANG HongYan WANG XiaoNing YANG Zhong ZHONG Yang WANG Hao CHE XiaoYan HUANG Zhong LAN Ke SUN Bing WU Fan YUAN ZhenAn ZHANG Xi ZHOU XiaoNong ZHOU JiaHai MA ZhiYong TONG GuangZhi GUO YaJun ZHAO GuoPing LI YiXue CAO ZhiWei | 2009 | Chinese Science Bulletin2009，54，13： | 2 |
| 7 | Identification of two novel B cell epitopes on porcine epidemic diarrhea virus spike protein显示文摘 | Dongbo Sun Li Feng Hongyan Shi Jianfei Chen Xiaochen Cui Hongyan Chen Shengwang Liu Youen Tong Yunfeng Wang Guangzhi Tong | 2008 | Veterinary Microbiology2008，，1： | 2 |
| 8 | Population amount risk assessment of extreme precipitation-induced landslides based on integrated machine learning model and scenario simulation显示文摘In this study,the future landslide population amount risk(LPAR)is assessed based on integrated machine learning models(MLMs)and scenario simulation techniques in Shuicheng County,China.Firstly,multiple MLMs were selected and hyperparameters were optimized,and the generated 11 models were crossintegrated to select the best model to calculate landslide susceptibility;by calculating precipitation for different extreme precipitation recurrence periods and combining the susceptibility results to assess the landslide hazard.Using the town as the basic unit,the exposure and vulnerability of the future landslide population under different Shared Socioeconomic Pathways(SSPs)scenarios in each town were assessed,and then combined with the hazard to estimate the LPAR in 2050.The results showed that the integrated model with the optimized random forest model as the combination strategy had the best comprehensive performance in susceptibility assessment.The distribution of hazard classes is similar to susceptibility,and with an increase in precipitation,the low-hazard area and high-hazard decrease and shift to medium-hazard and very high-hazard classes.The high-risk areas for future landslide populations in Shuicheng County are mainly concentrated in the three southwestern towns with high vulnerability,whereas the northern towns of Baohua and Qinglin are at the lowest risk class.The LPAR increased with the intensity of extreme precipitation.The LPAR differs significantly among the SSPs scenarios,with the lowest in the“fossil-fueled development(SSP5)”scenario and the highest in the“regional rivalry(SSP3)”scenario.In summary,the landslide susceptibility model based on integrated machine learning proposed in this study has a high predictive capability.The results of future LPAR assessment can provide theoretical guidance for relevant departments to cope with future socioeconomic development challenges and make corresponding disaster prevention and mitigation plans to prevent landslide risks from a developmental perspective. | Guangzhi Rong Kaiwei Li Zhijun Tong Xingpeng Liu Jiquan Zhang Yichen Zhang Tiantao Li | 2023 | Geoscience Frontiers2023，14，3： | 2 |
| 9 | A SYBR Green-based real-time RT-PCR assay for simple and rapid detection and differentiation of highly pathogenic and classical type 2 porcine reproductive and respiratory syndrome virus circulating in China显示文摘 | Zheng Chai Wenjun Ma Fang Fu Yuekun Lang Wei Wang Guangzhi Tong Qinfang Liu Xuehui Cai Xi Li | 2013 | Archives of Virology2013，，2： | 2 |
| 10 | A simple and rapid immunochromatographic strip test for monitoring antibodies to H5 subtype Avian Influenza Virus显示文摘 | Shangjin Cui Changmu Chen Guangzhi Tong | 2008 | Journal of Virological Methods2008，152，： | 1 |
| 11 | Identification of two novel B cell epitopes on porcine epidemic diarrhea virus spike protein显示文摘 | Dongbo Sun Li Feng Hongyan Shi Jianfei Chen Xiaochen Cui Hongyan Chen Shengwang Liu Youen Tong Yunfeng Wang Guangzhi Tong | 2008 | Veterinary Microbiology2008，，1： | 1 |
| 12 | Development and evaluation of a VP3-ELISA for the detection of goose and Muscovy duck parvovirus antibodies显示文摘 | Yun Zhang Yongfeng Li Ming Liu Dabing Zhang Dongchun Guo Chunguo Liu Haidong Zhi Xiaomei Wang Gang Li Na Li Shiguo Liu Wenhua Xiang Guangzhi Tong | 2009 | Journal of Virological Methods2009，，2： | 1 |
| 13 | Control of the PI3K/Akt pathway by porcine reproductive and respiratory syndrome virus显示文摘 | Liqian Zhu Shen Yang Wu Tong Jianping Zhu Hai Yu Yanjun Zhou Robert B. Morrison Guangzhi Tong | 2013 | Archives of Virology2013，，6： | 1 |
| 14 | N-Linked Glycosylation of GP5 of Porcine Reproductive and Respiratory Syndrome Virus Is Critically Important for Virus Replication In Vivo显示文摘 | Zuzhang Wei Tao Lin Lichang Sun Yanhua Li Xiaoming Wang Fei Gao Runxia Liu Chunyan Chen Guangzhi Tong Shishan Yuan | 2012 | Journal of Virology2012，，18： | 1 |
| 15 | Sulfated modification of the water-soluble polysaccharides from Polyporus albicans mycelia and its potential biological activities显示文摘 | Yongxu Sun Haitao Liang Guangzhi Cai Shuwen Guan Haibin Tong Xiudong Yang Jicheng Liu | 2008 | International Journal of Biological Macromolecules2008，，1： | 1 |
| 16 | Corrigendum to“Nucleolin interacts with the rabbit hemorrhagic disease virus replicase RdRp,nonstructural proteins p16 and p23,playing a role in virus replication”<[Virologica Sinica 37(2022)48-59]>显示文摘Due to our negligence,the original version of this article,published online on 12 January 2022,contained a mistake in Fig.4A.The lane ofβ-actin in Western blotting was misused.The correct Fig.4 is given below.We apologize for our oversight when preparing the figure and state that this does not change the scientific conclusions of the article in any way. | Jie Zhu Qiuhong Miao Hongyuan Guo Aoxing Tang Dandan Dong Jingyu Tang Fang Wang Guangzhi Tong Guangqing Liu | 2023 | Virologica Sinica2023，38，3： | 0 |
| 17 | Genetic characterization and pathogenicity of a reassortant Eurasian avian-like H1N1 swine influenza virus containing an internal gene cassette from 2009 pandemic H1N1 virus显示文摘Dear editor,Swine influenza virus(SIV)is a member of the Orthomyxoviridae family,influenza A virus genus,which can cause the swine influenza—an acute and highly contagious respiratory disease in pigs(Brown,2000;Kothalawala et al.,2006).The SIV was first observed in 1918 in the United States and the progenitor of the SIV was the H1N1 influenza virus which caused the Spanish influenza pandemic of 1918(Shope,1931). | Shuaiyong Wang Manzhu Wang Lingxue Yu Juan Wang Jiecong Yan Xinli Rong Yanjun Zhou Tongling Shan Wu Tong Guoxin Li Hao Zheng Guangzhi Tong Hai Yu | 2022 | Virologica Sinica2022，37，4： | 0 |
| 18 | Screening of Host Proteins Interacting with PorcineEpidemic Diarrhea Virus (PEDV) N Protein by YeastTwo-hybrid System显示文摘[Objective] The paper was to obtain host proteins interacting with porcine epidemic diarrhea virus(PEDV) N protein. [Method] The recombinant vector pGBKT7-N of PEDV N gene was constructed and used as the bait plasmid to screen the proteins interacting with N protein of PEDV from the cDNA library of porcine alveolar macrophage(PAM) by yeast two-hybrid method. [Result] There was no toxicity and self activation of bait protein in yeast hybridization system, and six proteins(FTH1, LGALS3, CORO1C, SNRPG, KRTAP5-3, ZNF598) interacting with N protein were indentified. It was confirmed that LGALS3 and SNRPG had specific interaction with N protein by return experiment and co-immunoprecipitation(CoIP) test. [Conclusion] The study lays a foundation for further studying the function of PEDV N protein and the pathogenic mechanism of PEDV. | Wang Zhongze Qin Cuili Kong Ning Zuo Yewen Wang Meng Zheng Hao Tong Wu Li Liwei Yu Hai Li Zhili Shan Tongling Tong Guangzhi Li Xue | 2018 | Animal Husbandry and Feed Science2018，10，4： | 0 |
| 19 | The Novel PRRSV Strain HBap4-2018 with a Unique Recombinant Pattern Is Highly Pathogenic to Piglets显示文摘Currently, various porcine reproductive and respiratory syndrome virus(PRRSV) variants emerged worldwide with different genetic characteristics and pathogenicity, increasing the difficulty of PRRS control. In this study, a PRRSV strain named HBap4-2018 was isolated from swine herds suffering severe respiratory disease with high morbidity in Hebei Province of China in 2018. The genome of HBap4-2018 is 15,003 nucleotides in length, and compared with NADC30-like PRRSV, nsp2 of HBap4-2018 has an additional continuous deletion of five amino acids. Phylogenetic analysis based on complete genome and ORF5 showed that HBap4-2018 belonged to lineage 8 of PRRSV-2, which was characterized by highly variable genome. However, HBap4-2018 was classified into lineage 1 based on phylogenetic analysis of nsp2,sharing higher amino acid homology(85.3%–85.5%) with NADC30-like PRRSV. Further analysis suggested that HBap4-2018 was a novel natural recombinant PRRSV with three recombinant fragments in the genome, of which highly pathogenic PRRSV(HP-PRRSV) served as the major parental strains, while NADC30-like PRRSV served as the minor parental strains. Five recombination break points were identified in nsp2, nsp3, nsp5, nsp9 and ORF6, respectively,presenting a novel recombinant pattern in the genome. Piglets inoculated with HBap4-2018 presented typical clinical signs with a mortality rate of 60%. High levels of viremia and obvious macroscopic and histopathological lesions in the lungs were observed, revealing the high pathogenicity of HBap4-2018 in piglets. | Pengfei Chen Xiangmei Tan Mengqin Lao Xia Wu Xiongwei Zhao Shuting Zhou Jiarong Yu Junrui Zhu Lingxue Yu Wu Tong Fei Gao Hai Yu Changlong Liu Yifeng Jiang Guangzhi Tong Yanjun Zhou | 2021 | Virologica Sinica2021，36，6： | 0 |
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