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| 1 | A novel m6A reader Prrc2a controls oligodendroglial specification and myelination显示文摘While N6-methyladenosine (m6A), the most abundant internal modification in eukaryotic mRNA, is linked to cell differentiation and tissue development, the biological significance of m6A modification in mammalian glial development remains unknown. Here, we identify a novel m6A reader, Prrc2a (Proline rich coiled-coil 2A), which controls oligodendrocyte specification and myelination. Nestin-Cre-mediated knockout of Prrc2a induces significant hypomyelination, decreased lifespan, as well as locomotive and cognitive defects in a mouse model. Further analyses reveal that Prrc2a is involved in oligodendrocyte progenitor cells (OPCs) proliferation and oligodendrocyte fate determination. Accordingly, oligodendroglial-lineage specific deletion of Prrc2a causes a similar phenotype of Nestin-Cre-mediated deletion. Combining transcriptome-wide RNA-seq, m6A-RIP-seq and Prrc2a RIP-seq analysis, we find that Olig2 is a critical downstream target gene of Prrc2a in oligodendrocyte development. Furthermore, Prrc2a stabilizes Olig2 mRNA through binding to a consensus GGACU motif in the Olig2 CDS (coding sequence) in an m6A-dependent manner. Interestingly, we also find that the m6A demethylase, Fto, erases the m6A modification of Olig2 mRNA and promotes its degradation. Together, our results indicate that Prrc2a plays an important role in oligodendrocyte specification through functioning as a novel m6A reader. These findings suggest a new avenue for the development of therapeutic strategies for hypomyelination-related neurological diseases. | Rong Wu Ang Li Baofa Sun Jian-Guang Sun Jinhua Zhang Ting Zhang Yusheng Chen Yujie Xiao Yuhao Gao Qingyang Zhang Jun Ma Xin Yang Yajin Liao Wei-Yi Lai Xiaolong Qi Shukun Wang Yousheng Shu Hai-Lin Wang Fengchao Wang Yun-Gui Yang Zengqiang Yuan | 2019 | Cell Research2019，29，1： | 32 |
| 2 | Mitochondrial calcium uniporter protein MCU is involved in oxidative stress-induced cell death显示文摘Mitochondrial 钙 uniporter (MCU ) 是在在真核细胞的房间的 mitochondrial 的保存 Ca 2+ transporter。然而,在氧化导致压力的房间死亡的 MCU 蛋白质的角色仍然保持不清楚。这里,我们证明宫外地表示的 MCU 是在局部性的 mitochondrial HeLa 和主要小脑的小粒神经原(CGN ) 。内长的 MCU 减少 mitochondrial Ca 2+ 举起追随者组织安刺激击倒并且稀释氧化应力在 HeLa 房间和 CGN 导致的房间死亡。我们也发现 MCU 与 VDAC1 交往并且调停在 CGN 的 VDAC1 导致 overexpression 的房间死亡。这发现证明那 MCU-VDAC1 建筑群调整 mitochondrial Ca 2+ 举起和氧化导致压力的 apoptosis,它可能表示为氧化应力的治疗学的目标联系了疾病。 | Yajin Liao Yumin Hao Hong Chen Qing He Zengqiang Yuan Jinbo Cheng | 2015 | Protein & Cell2015，6，6： | 12 |
| 3 | Chinese expert consensus on conversion therapy for hepatocellular carcinoma(2021 edition)显示文摘Recent advances in systemic and locoregional treatments for patients with unresectable or advanced hepatocellular carcinoma(HCC)have resulted in improved response rates.This has provided an opportunity for selected patients with initially unresectable HCC to achieve adequate tumor downstaging to undergo surgical resection,a‘conversion therapy’strategy.However,conversion therapy is a new approach to the treatment of HCC and its practice and treatment protocols are still being developed.Review the evidence for conversion therapy in HCC and develop consensus statements to guide clinical practice.Evidence review:Many research centers in China have accumulated significant experience implementing HCC conversion therapy.Preliminary findings and data have shown that conversion therapy represents an important strategy to maximize the survival of selected patients with intermediate stage to advanced HCC;however,there are still many urgent clinical and scientific challenges for this therapeutic strategy and its related fields.In order to summarize and learn from past experience and review current challenges,the Chinese Expert Consensus on Conversion Therapy for Hepatocellular Carcinoma(2021 Edition)was developed based on a review of preliminary experience and clinical data from Chinese and non-Chinese studies in this field and combined with recommendations for clinical practice.Sixteen consensus statements on the implementation of conversion therapy for HCC were developed.The statements generated in this review are based on a review of clinical evidence and real clinical experience and will help guide future progress in conversion therapy for patients with HCC. | Hui-Chuan Sun Jian Zhou Zheng Wang Xiufeng Liu Qing Xie Weidong Jia Ming Zhao Xinyu Bi Gong Li Xueli Bai Yuan Ji Li Xu Xiao-Dong Zhu Dousheng Bai Yajin Chen Yongjun Chen Chaoliu Dai Rongping Guo Wenzhi Guo Chunyi Hao Tao Huang Zhiyong Huang Deyu Li Gang Li Tao Li Xiangcheng Li Guangming Li Xiao Liang Jingfeng Liu Fubao Liu Shichun Lu Zheng Lu Weifu Lv Yilei Mao Guoliang Shao Yinghong Shi Tianqiang Song Guang Tan Yunqiang Tang Kaishan Tao Chidan Wan Guangyi Wang Lu Wang Shunxiang Wang Tianfu Wen Baocai Xing Bangde Xiang Sheng Yan Dinghua Yang Guowen Yin Tao Yin Zhenyu Yin Zhengping Yu Bixiang Zhang Jialin Zhang Shuijun Zhang Ti Zhang Yamin Zhang Yubao Zhang Aibin Zhang Haitao Zhao Ledu Zhou Wu Zhang Zhenyu Zhu Shukui Qin Feng Shen Xiujun Cai Gaojun Teng Jianqiang Cai Minshan Chen Qiang Li Lianxin Liu Weilin Wang Tingbo Liang Jiahong Dong Xiaoping Chen Xuehao Wang Shusen Zheng Jia Fan 无 | 2022 | Hepatobiliary Surgery and Nutrition2022，11，2： | 4 |
| 4 | The APR triangle:A practical zone in the Glissonean approach to laparoscopic anatomical right hepatectomy显示文摘Background:An understanding of vascular anatomy is crucial for the safe performance of laparoscopic anatomical liver excision.We discovered a triangular zone during the laparoscopic right liver surgery and termed this zone the APR triangle.The purpose of this study was to determine the probability of the existence of the APR triangle and elucidate its various forms.Methods:Analyzed three-dimensional image reconstructions of 66 individuals who underwent liver surgery and calculated the statistics for various types of APR triangles under various grouping settings.Results:The APR triangle was present in the majority of cases,with right hepatic vein trunk type in 68%and right hepatic vein branch type in 21%,respectively.The angle between the right anterior and right posterior hepatic pedicles(AP&PP)was at most between 45 and 90°(74%).There was a 35%chance that at least one of the AP&PP was longer than 2 cm,and a 39%chance that both were.The right posterior pedicle first branch would appear at the bifurcation of AP&PP in 13%only.Conclusions:The APR triangle is objectively present and may represent a practical zone for performing laparoscopic right hepatic anatomical resection more simply and safely. | Jie Chen Ziyu Zhang Rui Zhou Anzhi Wang Jun Cao Yajin Chen | 2022 | iLIVER2022，1，3： | 2 |
| 5 | Differential transcriptomic landscapes of multiple organs from SARS-CoV-2 early infected rhesus macaques显示文摘SARS-CoV-2 infection causes complicated clinical manifestations with variable multi-organ injuries,how-ever,the underlying mechanism,in particular immune responses in different organs,remains elusive.In this study,comprehensive transcriptomic alterations of 14 tissues from rhesus macaque infected with SARS-CoV-2 were analyzed.Compared to normal controls,SARS-CoV-2 infection resulted in dysregulation of genes involving diverse functions in various examined tissues/organs,with drastic transcriptomic changes in cerebral cortex and right ventricle.Intriguingly,cerebral cortex exhibited a hyperinflammatory state evidenced by sig-nificant upregulation of inflammation response-related genes.Meanwhile,expressions of coagulation,angio-genesis and fibrosis factors were also up-regulated in cerebral cortex.Based on our findings,neuropilin 1(NRP1),a receptor of SARS-CoV-2,was significantly elevated in cerebral cortex post infection,accompanied by active immune response releasing inflammatory factors and signal transmission among tissues,which enhanced infection of the central nervous system(CNS)in a positive feedback way,leading to viral encephalitis.Overall,our study depicts a multi-tissue/organ tran-scriptomic landscapes of rhesus macaque with early infection of SARS-CoV-2,and provides important insights into the mechanistic basis for COVID-19-asso-ciated clinical complications. | Chun-Chun Gao Man Li Wei Deng Chun-Hui Ma Yu-Sheng Chen Yong-Qiao Sun Tingfu Du Qian-Lan Liu Wen-Jie Li Bing Zhang Lihong Sun Si-Meng Liu Fengli Li Feifei Qi Yajin Qu Xinyang Ge Jiangning Liu Peng Wang Yamei Niu Zhiyong Liang Yong-Liang Zhao Bo Huang Xiao-Zhong Peng Ying Yang Chuan Qin Wei-Min Tong Yun-Gui Yang | 2022 | Protein & Cell2022，13，12： | 1 |
| 6 | Is HPB robotic-assisted surgery an evolution or a revolution in laparoscopy?显示文摘Robotic-assisted surgery technology demonstrates a new prospect for surgery.Its advantages are particularly prominent in specific surgery divisions or for specific surgical procedures,which has set off a new wave of surgical technology following laparoscopic surgery.Looking back at the history of surgical development over the past century,there have been many technological waves,some of which have become classics and standards,some of which are constantly evolving,and some of which have been gradually abandoned in practice.In the context of the increasingly mature and accurate laparoscopic surgery,which has become a standard operation of many types of surgery,the government,medical institutions and surgeons should carefully consider the role and future development of robotic-assisted surgery. | Jun Cao Yajin Chen | 2023 | Hepatobiliary Surgery and Nutrition2023，12，1： | 1 |
| 7 | A survey of Intel SGX and its applications显示文摘This paper presents a comprehensive survey on the development of Intel SGX(software guard extensions)processors and its applications.With the advent of SGX in 2013 and its subsequent development,the corresponding research works are also increasing rapidly.In order to get a more comprehensive literature review related to SGX,we have made a systematic analysis of the related papers in this area.We first search through five large-scale paper retrieval libraries by keywords(i.e.,ACM Digital Library,IEEE/IET Electronic Library,SpringerLink,Web of Science,and Elsevier Science Direct).We read and analyze a total of 128 SGX-related papers.The first round of extensive study is conducted to classify them.The second round of intensive study is carried out to complete a comprehensive analysis of the paper from various aspects.We start with the working environment of SGX and make a conclusive summary of trusted execution environment(TEE).We then focus on the applications of SGX.We also review and study multifarious attack methods to SGX framework and some recent security improvements made on SGX.Finally,we summarize the advantages and disadvantages of SGX with some future research opportunities.We hope this review could help the existing and future research works on SGX and its application for both developers and users. | Wei ZHENG Ying WU Xiaoxue WU Chen FENG Yulei SUI Xiapu LUO Yajin ZHOU | 2021 | Frontiers of Computer Science2021，15，3： | 1 |
| 8 | Ultra-broadband spatial light modulation with dual-resonance coupled epsilon-near-zero materials显示文摘It has been found that the dielectric constants of transparent conductive oxides(TCOs)can be adjusted in an extremely large range by tuning the carrier density.Due to the remarkable light confinement property of the epsilon-near-zero(ENZ)effect of TCOs,it has attracted extensive interests of light modulation.However,the operation wavelength bandwidth is usually limited by optical resonance that is applied to enhance the light-TCOs interaction.In this work,a dual-resonance light coupling scheme is proposed to expand the modulation depth-bandwidth product with almost one order-of-magnitude improvement.In a metallic subwavelength grating structure with deep trenches backed by a ground plane,the ENZ mode can be coupled to both magnetic resonance and Fabry-Perot resonance respectively by tuning the bias.Decent light modulation can be obtained in a large operation wavelength band covering two resonances by optimizing the dual-resonance configuration.Such a reconfigurable efficient broadband modulation is important for robust communication link and possesses remarkable capacity for wavelength division multiplexing. | Qin Chen Shichao Song Huacun Wang Li Liang Yajin Dong Long Wen | 2021 | Nano Research2021，14，8： | 0 |
| 9 | Tolerance and acceptance of hepatic venous pressure gradient measurement in cirrhosis(CHESS1904):An international multicenter study显示文摘Aim:To determine the tolerance and acceptance of hepatic venous pressure gradient(HVPG)measurements in patients with liver cirrhosis.Methods:This prospective international multicenter study included 271 patients with cirrhosis who were scheduled to undergo HVPG measurement between October 2019 and June 2020.Data related to the tolerance and acceptance of HVPG measurements were collected using descriptive questionnaires.Results:HVPG measurements were technically successful in all 271 patients,with 141(52.0%)undergoing HVPG measurement alone.The complication rate was 0.4%.Postoperative pain was significantly lower than preoperative expected pain(p<0.001)and intraoperative pain(p<0.001),and intraoperative pain was also significantly lower than preoperative expected pain(p=0.036).No,mild,moderate,severe,and intolerable discomfort scores were reported by 36.9%,44.6%,11.1%,6.3%,and 0.4%of these patients,respectively,during HVPG measurement and by 54.6%32.5%,11.4%,1.5%,and 0%,respectively,after HVPG measurement.Of these patients,39.5%had little understanding and 10%had no understanding of the value of HVPG measurement,with 35.1%and 4.1%regarding HVPG measurements as being of little or no help,respectively.Most patients reported that they would definitely(15.5%),probably(46.9%),or possibly(29.9%)choose to undergo additional HVPG measurements again,and 62.7%regarded the cost of the procedure as acceptable.Conclusion:HVPG measurement was safe and well‐tolerated in patients with cirrhosis,but patient education and communication are warranted to improve the acceptance of this procedure. | Jun‐Hui Sun He Zhao Haijun Zhang Lei Li NecatiÖrmeci Zi‐Niu Yu Xun Li Shuangxi Li Xujun Yang Huaping Wei Xiaoliang Zhu Zhengcong Zhang Yajin Wang Zhongwei Zhao Jianting Mao Qiaohong Wu Xiaole Sun Huiling Xiang Kefeng Jia Chao Yang Wei Wu Xiuqing Lin Haixin Yao Changzeng Zuo Jitao Wang Bo Zhang Chunqing Zhang Xiaoling Wu Guangchuan Wang Shengjuan Yao Ruihang Wang Li Zhou Hui Huan Qingli Tu Xue Pu Feng Zhang Qin Yin Linpeng Zhang Ying Guo Jian Wang Kohei Kotani Sawako Uchida‐Kobayashi Norifumi Kawada He Zhu Li Li Wei Wang Guo Zhang Lei Yu Xudong Cui Qingliang Zhu Hailong Zhang Xiaoli Hu Rafael OXimenes Adriano Gonçalves de Araújo Giulliano Gardenghi Yubao Zheng Zebin Wu Mingsheng Huang Xiaoyong Chen Jun Wu Feng Xie Yang Bo Shengjuan Hu Linke Ma Xiao Li Xiaolong Qi | 2022 | Portal Hypertension & Cirrhosis2022，1，1： | 0 |
| 10 | Splenic autotransplantation and oesophageal transection anastomosis in patients with portal hypertension (26 years clinical observation)显示文摘The surgical treatment methods for cirrhosis patients complicated with portal hypertension are complicated.In this study,we evaluated the effectiveness of a new treatment strategy:splenic auto-transplantation and oesophageal transection anastomosis on 274 patients from three aspects:clinical observation,splenic immunology and portal dynamics.From 1979 to 2005,274 cirrhosis patients with portal hypertension who underwent the new treatment strategy were followed up to observe different clinical indexes,which were then compared with those of the traditional surgery treatment.From 1999 to 2002,a randomized control trial(RCT)was performed on 40 patients to compare their immune function after operation.From 1994 to 2004,another RCT was carried out on 28 patients to compare the portal dynamics through three-dimensional dynamic contrast enhanced MR angiography(3D DEC MRA)investigation after operation.Among 274 patients(mean age 41.8 years),the emergency operative mortality(4.4%),selective operative mortality(2.2%),complication rate(17.9%),morbidity of hepatic encephalopathy(<1%),bleeding rate of portal hypertension gastritis(PHG)(9.1%),and morbidity of hepatic carcinoma(8%)were similar to those under traditional operation;the spleen immunology function(Tuftsin,IgM)decreased among the groups 2 months after operation.Through 3D DCE MRA,the cross section area,the velocity and volume of blood flow of main portal vein decrease significantly after operation in both groups,the auto transplantation group was significantly lower in velocity and volume of blood flow than in the control group.Splenic auto transplantation and esophageal transection anastomosis are a safe,effective,and reasonable treatment strategy for portal hypertension with varicial bleeding.It can not only correct hypersplenism but also completely stanch blood,and auto transplanted spleen in the retroperitoneal space can preserve immune function and establish abroad collateral circulation. | CHEN Jisheng HUO Jinshan ZHANG Hongwei SHANG Changzhen CHEN Rufu ZHANG Jie Obetien Mapudengo CHEN Yajin ZHANG Lei | 2007 | Frontiers of Medicine2007，1，1： | 0 |
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