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| 1 | Approaches to establish Q-markers for the quality standards of traditional Chinese medicines显示文摘Traditional Chinese medicine(TCM) has played a pivotal role in maintaining the health of Chinese people and is now gaining increasing acceptance around the global scope. However, TCM is confronting more and more concerns with respect to its quality. The intrinsic 'multicomponent and multitarget' feature of TCM necessitates the establishment of a unique quality and bioactivity evaluation system, which is different from that of the Western medicine. However, TCM is investigated essentially as'herbal medicine' or 'natural product', and the pharmacopoeia quality monographs are actually chemicalmarkers-based, which can ensure the consistency only in the assigned chemical markers, but, to some extent, have deviated from the basic TCM theory. A concept of 'quality marker'(Q-marker), following the 'property-effect-component' theory, is proposed. The establishment of Q-marker integrates multidisciplinary technologies like natural products chemistry, analytical chemistry, bionics, chemometrics,pharmacology, systems biology, and pharmacodynamics, etc. Q-marker-based fingerprint and multicomponent determination conduce to the construction of more scientific quality control system of TCM.This review delineates the background, definition, and properties of Q-marker, and the associated technologies applied for its establishment. Strategies and approaches for establishing Q-marker-based TCM quality control system are presented and highlighted with a few TCM examples. | Wenzhi Yang Yibei Zhang Wanying Wu Luqi Huang Dean Guo Changxiao Liu | 2017 | Acta Pharmaceutica Sinica B2017，7，4： | 51 |
| 2 | Identification and differentiation of Panax ginseng,Panax quinquefolium, and Panax notoginseng by monitoring multiple diagnostic chemical markers显示文摘To differentiate traditional Chinese medicines(TCM) derived from congeneric species in TCM compound preparations is usually challenging. The roots of Panax ginseng(PG), Panax quinquefolium(PQ) and Panax notoginseng(PN) are used as popular TCM. They contain similar triterpenoid saponins(ginsenosides) as the major bioactive constituents. Thus far, only a few chemical markers have been discovered to differentiate these three species. Herein we present a multiple marker detection approach to effectively differentiate the three Panax species, and to identify them in compound preparations. Firstly, 85 batches of crude drug samples(including 32 PG, 30 PQ, and 23 PN) were analyzed by monitoring 40 major ginsenosides in the extracted ion chromatograms(EICs) using a validated LC–MS fingerprinting method. Secondly, the samples were clustered into different groups by pattern recognition chemometric approaches using PLS-DA and OPLS-DA models, and17 diagnostic chemical markers were discovered. Aside from the previously known Rf and p-F11, ginsenoside Rs1 could be a new marker to differentiate PG from PQ. Finally, the above multiple chemical markers were used to identify the Panax species in 60 batches of TCM compound preparations. | Wenzhi Yang Xue Qiao Kai Li Jingran Fan Tao Bo De-an Guo Min Ye | 2016 | Acta Pharmaceutica Sinica B2016，6，6： | 18 |
| 3 | Chinese Stroke Association guidelines for clinical management of cerebrovascular disorders:executive summary and 2019 update of the management of high-risk population显示文摘Aim Cerebrovascular disease is the leading cause of death and disability in China,causing a huge burden among patients and their families.Hence,stroke prevention is critical,especially in the high-risk population.Here,we present the evidence-based guideline suitable for the Chinese population.Methods Literature search of PubMed and Cochrane library(from January 1964 to June 2019)was done.After thorough discussion among the writing group members,recommendations were listed and summarised.This guideline was reviewed and discussed by the fellow writing committees of the Chinese Stroke Association’s Stroke.Results This evidence-based guideline was written in three parts:controlling the risk factors of stroke,utilisation of antiplatelet agents and assessing the risks of first-ever stroke.All recommendations were listed along with the recommending classes and levels of evidence.Conclusions This guideline provides recommendations for primary prevention of cerebrovascular disease among high-risk population in China.Controlling related risk factors,appropriately using antiplatelet agents,assessing the risk of developing first-ever stroke should help reduce the rate of cerebrovascular disease in China. | Yilong Wang Shangrong Han Haiqiang Qin Huaguang Zheng Bin Jiang Yong Cao Yuan Gao Ling Guan Qian Jia Yong Jiang Yuming Jiao Shuya Li Yapeng Li Zixiao Li Wei Liu Xiaojuan Ru Dongling Sun Haixin Sun Penglian Wang Tingting Wang Lixia Zong Lei Guo Xuewei Xie Yuming Xu Yuyuan Xu Xiaomeng Yang Yingying Yang Mengyuan Zhou Wenzhi Wang Chinese Stroke Association Stroke Council Guideline Writing Committee | 2020 | Stroke & Vascular Neurology2020，5，3： | 11 |
| 4 | Offline two-dimensional liquid chromatography coupled with ion mobility-quadrupole time-of-flight mass spectrometry enabling fourdimensional separation and characterization of the multicomponents from white ginseng and red ginseng显示文摘Inherent complexity of plant metabolites necessitates the use of multi-dimensional information to accomplish comprehensive profiling and confirmative identification.A dimension-enhanced strategy,by offline two-dimensional liquid chromatography/ion mobility-quadrupole time-of-flight mass spectrometry(2 D-LC/IM-QTOF-MS)enabling four-dimensional separations(2 D-LC,IM,and MS),is proposed.In combination with in-house database-driven automated peak annotation,this strategy was utilized to characterize ginsenosides simultaneously from white ginseng(WG)and red ginseng(RG).An offline 2 DLC system configuring an Xbridge Amide column and an HSS T3 column showed orthogonality 0.76 in the resolution of ginsenosides.Ginsenoside analysis was performed by data-independent high-definition MSE(HDMSE)in the negative ESI mode on a Vion?IMS-QTOF hybrid high-resolution mass spectrometer,which could better resolve ginsenosides than MSEand directly give the CCS information.An in-house ginsenoside database recording 504 known ginsenosides and 58 reference compounds,was established to assist the identification of ginsenosides.Streamlined workflows,by applying UNIFI?to automatedly annotate the HDMSEdata,were proposed.We could separate and characterize 323 ginsenosides(including 286 from WG and 306 from RG),and 125 thereof may have not been isolated from the Panax genus.The established 2 D-LC/IM-QTOF-HDMSEapproach could also act as a magnifier to probe differentiated components between WG and RG.Compared with conventional approaches,this dimensionenhanced strategy could better resolve coeluting herbal components and more efficiently,more reliably identify the multicomponents,which,we believe,offers more possibilities for the systematic exposure and confirmative identification of plant metabolites. | Tiantian Zuo Chunxia Zhang Weiwei Li Hongda Wang Ying Hu Wenzhi Yang Li Jia Xiaoyan Wang Xiumei Gao Dean Guo | 2020 | Journal of Pharmaceutical Analysis2020，10，6： | 9 |
| 5 | Comparison of the inhibition potentials of icotinib and erlotinib against human UDP-glucuronosyltransferase 1A1显示文摘UDP-glucuronosyltransferase 1A1(UGT1A1) plays a key role in detoxification of many potentially harmful compounds and drugs. UGT1A1 inhibition may bring risks of drug–drug interactions(DDIs), hyperbilirubinemia and drug-induced liver injury. This study aimed to investigate and compare the inhibitory effects of icotinib and erlotinib against UGT1A1, as well as to evaluate their potential DDI risks via UGT1A1 inhibition. The results demonstrated that both icotinib and erlotinib are UGT1A1 inhibitors, but the inhibitory effect of icotinib on UGT1A1 is weaker than that of erlotinib. The IC_(50) values of icotinib and erlotinib against UGT1A1-mediated NCHN-O-glucuronidation in human liver microsomes(HLMs) were 5.15 and 0.68 μmol/L, respectively. Inhibition kinetic analyses demonstrated that both icotinib and erlotinib were non-competitive inhibitors against UGT1A1-mediated glucuronidation of NCHN in HLMs, with the Kivalues of 8.55 and 1.23 μmol/L, respectively. Furthermore, their potential DDI risks via UGT1A1 inhibition were quantitatively predicted by the ratio of the areas under the concentration–time curve(AUC) of NCHN. These findings are helpful for the medicinal chemists todesign and develop next generation tyrosine kinase inhibitors with improved safety, as well as to guide reasonable applications of icotinib and erlotinib in clinic, especially for avoiding their potential DDI risks via UGT1A1 inhibition. | Xuewei Cheng Xia Lv Hengyan Qu Dandan Li Mengmeng Hu Wenzhi Guo Guangbo Ge Ruihua Dong | 2017 | Acta Pharmaceutica Sinica B2017，7，6： | 7 |
| 6 | Intelligent identification of two-dimensional nanostructures by machine-learning optical microscopy显示文摘Two-dimensional (2D)materials and their heterostructures,with wafer-scale synthesis methods and fascinating properties,have attracted significant interest and triggered revolutions in corresponding device applications.However,facile methods to realize accurate,intelligent,and large-area characterizations of these 2D nanostructures are still highly desired.Herein,we report the successful application of machine-learning strategy in the optical identification of 2D nanostructures.The machine-leaming optical identification (MOI)method endows optical microscopy with intelligent insight into the characteristic color information of 2D nanostructures in the optical photograph.The experimental results indicate that the MOI method enables accurate,intelligent,and large-area characterizations of graphene,molybdenum disulfide,and their heterostructures, including identifications of the thickness,existence of impurities,and even stacking order.With the convergence of artificial intelligence and nanoscience,this intelligent identification method can certainly promote fundamental research and wafer-scale device applications of 2D nanostructures. | Xiaoyang Lin Zhizhong Si Wenzhi Fu Jianlei Yang Side Guo Yuan Cao Jin Zhang Xinhe Wang Peng Liu Kaili Jiang Weisheng Zhao | 2018 | Nano Research2018，11，12： | 5 |
| 7 | Chinese expert consensus on conversion therapy for hepatocellular carcinoma(2021 edition)显示文摘Recent advances in systemic and locoregional treatments for patients with unresectable or advanced hepatocellular carcinoma(HCC)have resulted in improved response rates.This has provided an opportunity for selected patients with initially unresectable HCC to achieve adequate tumor downstaging to undergo surgical resection,a‘conversion therapy’strategy.However,conversion therapy is a new approach to the treatment of HCC and its practice and treatment protocols are still being developed.Review the evidence for conversion therapy in HCC and develop consensus statements to guide clinical practice.Evidence review:Many research centers in China have accumulated significant experience implementing HCC conversion therapy.Preliminary findings and data have shown that conversion therapy represents an important strategy to maximize the survival of selected patients with intermediate stage to advanced HCC;however,there are still many urgent clinical and scientific challenges for this therapeutic strategy and its related fields.In order to summarize and learn from past experience and review current challenges,the Chinese Expert Consensus on Conversion Therapy for Hepatocellular Carcinoma(2021 Edition)was developed based on a review of preliminary experience and clinical data from Chinese and non-Chinese studies in this field and combined with recommendations for clinical practice.Sixteen consensus statements on the implementation of conversion therapy for HCC were developed.The statements generated in this review are based on a review of clinical evidence and real clinical experience and will help guide future progress in conversion therapy for patients with HCC. | Hui-Chuan Sun Jian Zhou Zheng Wang Xiufeng Liu Qing Xie Weidong Jia Ming Zhao Xinyu Bi Gong Li Xueli Bai Yuan Ji Li Xu Xiao-Dong Zhu Dousheng Bai Yajin Chen Yongjun Chen Chaoliu Dai Rongping Guo Wenzhi Guo Chunyi Hao Tao Huang Zhiyong Huang Deyu Li Gang Li Tao Li Xiangcheng Li Guangming Li Xiao Liang Jingfeng Liu Fubao Liu Shichun Lu Zheng Lu Weifu Lv Yilei Mao Guoliang Shao Yinghong Shi Tianqiang Song Guang Tan Yunqiang Tang Kaishan Tao Chidan Wan Guangyi Wang Lu Wang Shunxiang Wang Tianfu Wen Baocai Xing Bangde Xiang Sheng Yan Dinghua Yang Guowen Yin Tao Yin Zhenyu Yin Zhengping Yu Bixiang Zhang Jialin Zhang Shuijun Zhang Ti Zhang Yamin Zhang Yubao Zhang Aibin Zhang Haitao Zhao Ledu Zhou Wu Zhang Zhenyu Zhu Shukui Qin Feng Shen Xiujun Cai Gaojun Teng Jianqiang Cai Minshan Chen Qiang Li Lianxin Liu Weilin Wang Tingbo Liang Jiahong Dong Xiaoping Chen Xuehao Wang Shusen Zheng Jia Fan 无 | 2022 | Hepatobiliary Surgery and Nutrition2022，11，2： | 4 |
| 8 | Recent advances in catalytic production of sugar alcohols and their applications显示文摘Conversion of non-edible biomass into fuels and value-added chemicals has achieved great attention to cope the world's energy requirements. Lignocellulose based sugar alcohols such as sorbitol, mannitol, xylitol, and erythritol can be potentially used as emerging fuels and chemicals. These sugar alcohols can be converted into widely used products(e.g. polymer synthesis, food and pharmaceuticals industry). The heterogeneous catalytic production of sugar alcohols from renewable biomass provides a safe and sustainable approach. Hydrolysis, coupled with hydrogenation and hydrogenolysis has been proved to be more effective strategy for sugar alcohols production from biomass. This review summarizes the recent advances in biomass upgrading reactions for the production of sugar alcohols and their comprehensive applications. | Bakht Zada Mengyuan Chen Chubai Chen Long Yan Qing Xu Wenzhi Li Qingxiang Guo Yao Fu | 2017 | Science China Chemistry2017，60，7： | 3 |
| 9 | Application of Modified Progressive Hedging for Stochastic Unit Commitment in Electricity-Gas Coupled Systems显示文摘The increasing number of gas-fired units has significantly intensified the coupling between electric and gas power networks.Traditionally,nonlinearity and nonconvexity in gas flow equations,together with renewable-induced stochasticity,resulted in a computationally expensive model for unit commitment in electricity-gas coupled integrated energy systems(IES).To accelerate stochastic day-ahead scheduling,we applied and modified Progressive Hedging(PH),a heuristic approach that can be computed in parallel to yield scenario-independent unit commitment.Through early termination and enumeration techniques,the modified PH algorithm saves considerable com,putational time for certain generation cost settings or when the scale of the IES is large.Moreover,an adapted second-order cone relaxation(SOCR)is utilized to tackle the nonconvex gas flow equation.Case studies were performed on the IEEE 24.bus system/Belgium 20-node gas system and the IEEE 118-bus system/Belgium 20-node gas system.The computational efficiency when employing PH is 188 times that of commercial software,and the algorithm even outperforms Benders Decomposition.At the same time,the gap between the PH algorithm and the benchmark is less than 0.01% in both IES systems,which proves that the solutions produced by PH reach acceptable optimality in this stochastic UC problem. | Haizhou Liu Xinwei Shen Qinglai Guo Hongbin Sun Mohammad Shahidehpour Wenzhi Zhao Xinyi Zhao | 2021 | CSEE Journal of Power and Energy Systems2021，7，4： | 3 |
| 10 | Planar or quasi-planar octa-and ennea-coordinate aluminum and gallium in boron rings显示文摘An ab initio theoretical investigation has been performed on planar or quasi-planar octa-and ennea-coordinate Al and Ga centered in X@B8- and X@B9 (X=Al, Ga). These high symmetry molecular wheels all turned out to be true minima of the systems and σ+π double aromatic in nature, similar to the previously characterized D8h B@B8- both electronically and geometrically. Adiabatic and vertical detachment energies of the anions and the ionization potentials of the neutrals have been calculated to aid their eventual experimental identification. | GUO JinChang YAO WenZhi LI Zhuo LI SiDian | 2009 | Science China Chemistry2009，52，5： | 2 |
| 11 | Nerve growth factor precursor and sortilin effects on perihematomal brain tissue and the relationship to secondary cell apoptosis显示文摘BACKGROUND: Neuronal apoptosis in perihematomal brain tissues following intracerebral hemorrhage is strongly related to the formation of a compound signal pathway between nerve growth factor precursor (proNGF), p75NTR, and sortilin receptor. Sortilin acts as a co-receptor and molecular switch governing the p75NTR-mediated pro-apoptotic signal induced by proNGF. OBJECTIVE: To investigate proNGF and sortilin expressions in perihematomal brain tissues following intracerebral hemorrhage, and to study the effects of proNGF and sortilin on secondary cell apoptosis. DESIGN, TIME AND SETTING: A paired, comparison study was performed at the Laboratory of Histology and Embryology, Xi'an Jiaotong University from October 2007 to September 2008. MATERIALS: Brain tissue samples were obtained from 15 patients with intracerebral hemorrhage, who were treated at the Department of Neurosurgery, First Affiliated Hospital, Medical College of Xi'an Jiaotong University from October 2007 to March 2008. Rabbit anti-proNGF polyclonal antibody was provided by Chemicon, USA; rabbit antisortilin polyclonal antibody by Abcam, UK; and TUNEL kit by Promega, USA. METHODS: Perihematomal brain tissues selected 0.5 cm from the hemorrhage area were considered to be the hemorrhage group, while brain tissues from the middle temporal gyrus served as the control group. MAIN OUTCOME MEASURES: Histopathological changes were detected using hematoxylin-eosin staining, cell apoptosis was determined using the TUNEL method, and proNGF and sortilin ex- pressions were determined using immunohistochemistry. RESULTS: Edema was clearly observed in perihematomal brain tissues, and infiltration of inflammatory cells was visible, with the presence of irregular and necrotic bodies. The apoptotic rate in the hemorrhage group was significantly greater than in the control group (P < 0.01). Moreover, sortilin expression significantly increased (P < 0.01), but proNGF expression remained unchanged (P > 0.05). Correlation analysis suggested that sortilin expression positively correlated with apoptosis (rs = 0.648, P < 0.01). CONCLUSION: proNGF expression was stable, but sortilin expression increased in perihematomal brain tissues following intracerebral hemorrhage, suggesting that sortilin acted as a co-receptor and molecular switch to govern p75NTR-mediated cell apoptosis. | Shiwen Guo Yuliang Han Gang Bao Wenzhi Li | 2010 | Neural Regeneration Research2010，5，1： | 2 |
| 12 | Soil organic carbon storages and bacterial communities along a restored mangrove soil chronosequence in the Jiulong River Estuary:From tidal flats to mangrove afforestation显示文摘Among many ecological services provided by mangrove ecosystems,soil organic carbon(SOC)storages have recently received much attention owing to the increasing atmospheric partial pressure of dissolved CO_(2)(pCO_(2)).Bacteria are fundamental to ecosystem functions and strongly influence the coupling of coastal carbon,nitrogen,and sulfur cycling in soils.The SOC storage and bacterial communities along a restored mangrove soil chronosequence in the Jiulong River Estuary were explored using the 16S rDNA sequencing technique.The results showed the SOC storage in the 100 cm soil profile was 103.31±5.87 kg C m^(−2)and 93.10±11.28 kg C m^(−2)for mangroves with afforestation ages of 36 and 60 years,respectively.The total nitrogen(TN)and total sulfur(TS)contents exhibited significant correlations with the SOC in the mangrove soils,but only TN and SOC showed significant correlation in tidal flat soils.Although the tidal flats and mangroves occupied the contiguous intertidal zone within several kilometers,the variations in the SOC storage along the restored mangrove soil chronosequence were notably higher.The Functional Annotation of Prokaryotic Taxa(FAPROTAX)database was used to annotate the metabolic functions of the bacteria in the soils.The annotation revealed that only four metabolic functions were enriched with a higher relative abundance of the corresponding bacteria,and these enriched functions were largely associated with sulfate reduction.In addition,the specifically critical bacterial taxa that were associated with the SOC accumulation and nutrient cycling,shaped the distinct metabolic functions,and consequently facilitated the SOC accumulation in the mangrove soils with various afforestation ages.The general homogenization of the microbial community and composition along the intertidal soil chronosequence was primarily driven by the reciprocating tidal flows and geographical contiguity. | Feifei Wang Rui Guo Ning Zhang Shengchang Yang Wenzhi Cao | 2023 | Fundamental Research2023，3，6： | 1 |
| 13 | Efficient,deep red phosphorescent OLEDs using 1,2,4-thiadiazole core-based novel bipolar host with low efficiency roll-off显示文摘A series of 1,2,4-thiadiazole core-based bipolar materials,2,2'-(1,2,4-thiadiazole-3,5-diyl)bis(N,N- diphenylaniline)(o-TPATHZ),3,3'-(1,2,4-thiadiazole-3,5-diyl)bis(N,N-diphenylaniline)(m-TPATHZ)and 4,4'-(1,2,4-thiadiazole-3,5-diyl)bis(N,N-diphenylaniline)(1)-TPATHZ)were developed as the host matrixes for the deep red phosphorescent emitters tris(1-phenylisoqiuno-line)iridium (It(pie03)and [bis(2-methyldibenzo-[f,h]-quinoxaline)Ir(III)(acetylacetonate)](Ir(MDQ)2(acac)).By systematic studying,we demonstrated that there are two types of charge-trapping effect within the emissive layers through adjusting the host-guest compatibility.And,it is revealed that a symmetric charge-trapping effect can contribute to realizing a stable charge-balance,which led to a mitigating efficiency roll-off at high current density. Consequently,a maximum external quantum efficiency (EQE)of 16.2%was achieved by an optimized device with p-TPATHZ-Ir(piq)3emissive layer.Remarkably,the EQE still remained as high as 15.7%at the high luminance of 1000cd/m^2. | Runda GUO Wenzhi ZHANG Qing ZHANG Xialei LV Lei WANG | 2018 | Frontiers of Optoelectronics2018，11，4： | 1 |
| 14 | Polymorphisms of DNA repair gene XRCC1 and hepatocellular carcinoma risk among East Asians: a meta-analysis显示文摘 | Jie Li Zhenzhen Li Liushun Feng Wenzhi Guo Shuijun Zhang | 2013 | Tumor Biology2013，，1： | 1 |
| 15 | Optimal analysis conditions for sperm motility parameters with a CASA system in a passerine bird, Passer montanus显示文摘Background:Sperm motility parameters,which can be measured objectively and repeatedly by a computer-assisted sperm analysis(CASA)system,are important indicators of sperm quality.However,the sperm motility parameters assessed by a CASA system can be affected by various factors,including instrument components and settings,sperm preparation or analysis procedures.To date,no standardized protocol is available that would permit to assess sperm kinetic characteristics in passerine birds and this lack precludes any comparison of sperm swimming ability and sperm quality across species.Methods:In this study,we chose the Tree Sparrow(Passer montanus)as the object to evaluate sperm motility parameters,including sperm motility,sperm velocity and sperm movement trajectory,at different analysis time,temperatures and pH using the WLJY-9000 CASA system.Results:Sperm motility parameters remained statistically unchanged at 1‒9 min.Progressive motility was similar at 38℃ and 40℃,but a greater percentage of slow progressive sperm was detected at 38℃ compared to 40℃ and 42℃.Additionally,progressive motility was lower and immotility was higher at 42℃than 38℃and/or 40℃(close to the body temperature of the Tree Sparrow).The percentages of rapid progressive sperm,progressive sperm and immotile sperm were statistically similar at pH 7.0,7.5 and 8.0 with the exception of lower percentage of progressive sperm at pH 7.0 compared to pH 7.5.In addition,slower sperm velocity and worse sperm movement trajectory were found at pH 6.0 and 9.0 than those at pH 8.0,7.5 or 7.0.Conclusions:Our study indicates that the ideal conditions for sperm motility parameters assessment in Tree Sparrow are obtained between 1 and 9 min after dilution,an environment at body temperature(40℃)and a pH around 7.5-8.0.The results of this study provide a reference for the evaluation of sperm characteristics and sperm quality using a CASA system in passerine birds. | Ying Yang Yingmei Zhang Jian Ding Shiwei Ai Rui Guo Xiaojuan Bai Wenzhi Yang | 2019 | Avian Research2019，10，4： | 1 |
| 16 | The Effect of FOXP3^(+) Regulatory T Cells on Infectious and Inflammatory Diseases显示文摘CD4^(+)CD25^(+)FOXP3^(+)regulatory T cells(Tregs)contribute to the maintenance of immune homeostasis and tolerance in the body.The expression levels and functional stability of FOXP3 control the function and plasticity of Tregs.Tregs critically impact infectious diseases,especially by regulating the threshold of immune responses to pathogenic microorganisms.The functional regulatory mechanism and cell-specific surface markers of Tregs in different tissues and inflammatory microenvironments have been investigated in depth,which can provide novel ideas and strategies for immunotherapies targeting infectious diseases. | Yakun Bai Fang Gao Dan Li Suyuan Ji Shuijun Zhang Wenzhi Guo Bin Li | 2021 | Infectious Microbes & Diseases2021，3，4： | 1 |
| 17 | LncRNA-m18as1 competitively binds with miR-18a-5p to regulate follicle-stimulating hormone secretion through the Smad2/3 pathway in rat primary pituitary cells显示文摘Long noncoding RNAs(lncRNAs)are expressed in different species and different tissues,and perform different functions,but little is known about their involvement in the synthesis or secretion of follicle-stimulating hormone(FSH).In general,we have revealed lnc RNA-micro RNA(mi RNA)-messenger RNA(m RNA)interactions that may play important roles in rat primary pituitary cells.In this study,a new lncRNA was identified for the first time.First,we analyzed the gene expression of lncRNA-m18as1 in different tissues and different stages by reverse transcription-quantitative polymerase chain reaction(RT-qPCR)and observed the localization of lncRNA-m18as1 with fluorescence in situ hybridization,which indicated that this lncRNA was distributed mainly in the cytoplasm.Next,we used RT-qPCR and enzyme-linked immunosorbent assay(ELISA)to analyze the regulation of FSH synthesis and secretion after overexpression or knockdown of lncRNA-m18as1 and found that lncRNA-m18as1 was positively correlated with FSH synthesis and secretion.In addition,mothers against decapentaplegic homolog 2(Smad2)was highly expressed in our sequencing results.We also screened miR-18a-5p from our sequencing results as a miRNA that may bind to lncRNA-m18as1 and Smad2.We used RNA immunoprecipitation-qPCR(RIP-qPCR)and/or dual luciferase assays to confirm that lncRNA-m18as1 interacted with miR-18a-5p and miR-18a-5p interacted with Smad2.Fluorescence in situ hybridization(FISH)showed that lncRNA-m18as1 and miR-18a-5p were localized mainly in the cytoplasm.Finally,we determined the relationship among lncRNA-m18as1,miR-18a-5p,and the Smad2/3 pathway.Overall,we found that lncRNA-m18as1 acts as a molecular sponge of miR-18a-5p to regulate the synthesis and secretion of FSH through the Smad2/3 pathway. | Weidi ZHANG Wenzhi REN Dongxu HAN Guokun ZHAO Haoqi WANG Haixiang GUO Yi ZHENG Zhonghao JI Wei GAO Bao YUAN | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，6： | 0 |
| 18 | Cash gifts,perception of social sphere and distribution of water resources for rural farmers in a typical oasis area in Northwest China显示文摘Face-to-face interviews(n=860) were conducted to determine cash-gift behavior, cognition and complexity of social relations, and family water consumption for agricultural production in a typical oasis area in Northwest China. The survey results revealed cash gifts to be the second-largest expenditure(1,330 Yuan) for rural households, far beyond the average spending on health(854.1 Yuan) or education(890.8 Yuan). The amount of cash-gift expenditures indirectly reflects a family's social relations level, and our developed Social Relations Extent Coefficient—based on cash-gift and food expenditures—can directly reflect the extent or level of these relationships. Different types of farmers or farming families have different coefficients. The coefficient also shows how social station affects a family's water resource utilization, and how water is distributed among different types of farmers. Despite the current inequities, however, promotion of better agricultural techniques and large-scale cultivation in this area could help distribute water resources more equitably and effectively. | QianTao Zhu WenZhi Zhao Jian Guo Hu Liu | 2018 | Research in Cold and Arid Regions2018，10，2： | 0 |
| 19 | Fluid-structure interaction simulation for multi-body flexible morphing structures显示文摘The multi-body flexible morphing airfoil can improve the aerodynamic characteristics based on different flight missions continuously.Recently researches have focused on the unsteady aerodynamic characteristics of flexible wings under passive actuation.However,the unsteady aerodynamic characteristics with the fluid-structure interaction effects in the multi-body active actuation process of morphing airfoil deserve further investigation.In this paper,a fluid-structure coupled simulation method for multi-body flexible morphing airfoil with active actuation subsystem was investigated,and the aerodynamic characteristics during deformation were compared with different skin flexibility,flow field environment,actuation mode and actuation time.The numerical results show that for the steady aerodynamic,the skin flexibility can improve the stability efficiency.In the unsteady process,the change trend of the transient lift coefficient and pitching moment are consistent with those of the active drive characteristics,while the instantaneous lift-drag ratio coefficient is greatly affected by the driving mode and can be improved by increasing the driving duration. | Wenzhi GUO Yongtao SHUI Lu NIE Gang CHEN | 2024 | Chinese Journal of Aeronautics2024，37，2： | 0 |
| 20 | SIMULTANEOUS QUANTITATION OF FIVE PANAX NOTOGINSENG SAPONINS IN EIGHT CHINESE PATENT MEDICINES BY MULTI HEART-CUTTING TWODIMENSIONAL LIQUID CHROMATOGRAPHY显示文摘Current China Pharmacopoeia(ChP)standards employ diversified and case-dependent assay methods to evaluate the quality of different Chinese patent medicines(CPM)that contain Panax notoginseng as the monarch drug.This study is aimed to develop a two-dimensional liquid chromatography(2D LC)based assay approach to unify and simplify the quality assessment of Notoginseng containing CPMs.Multi heart-cutting 2D LC(MHC-2D LC)was used to separate and quantify five major | Changliang Yao Wenzhi Yang Wanying Wu Jinjun Hou Jingxian Zhang Yanhai Zhang Dean Guo | 2015 | World Journal of Traditional Chinese Medicine2015，1，4： | 0 |
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