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| 1 | Production of transgenic mice carrying green fluorescence protein gene by a lentiviral vector-mediated approach显示文摘pseudo-lentivirus,带表示盒子的绿荧光蛋白质(GFP ) ,被注入仙子卵黄在移植进收养的母亲的输卵管前的鼠科的使肥沃的卵母细胞的空格。GFP 转基因的小狗然后被获得。由 PCR 扩大,荧光灯的显微镜学和流动帮助了排序分析的 cytometry,我们发现 transgene 的集成率在上面被估计 40% 。实时 PCR 分析显示综合 GFP 盒子的拷贝数字在 40 附近。在 situ 杂交分析荧光灯证明集成模式随机却可继承。有多集成地点和各种各样的表示层次的转基因的老鼠象研究一样在实践拥有了大价值。此处报导的途径提供一个有效方法给产生并且屏蔽转基因的老鼠紧张的有效方法。 | ZHANG Jingzhi GUO Xinbing XIE Shuyang ZHU Yiwen HUANG Ying WANG Shu REN Zhaorui | 2006 | Progress in Natural Science:Materials International2006，16，8： | 6 |
| 2 | Bevacizumab biosimilar LY01008 compared with bevacizumab(Avastin)as first-line treatment for Chinese patients with unresectable,metastatic,or recurrent non-squamous non-small-cell lung cancer:A multicenter,randomized,double-blinded,phase Ⅲ trial显示文摘Background:Previous studies have demonstrated the preclinical pharmacological and toxicological consistency,and clinical pharmacokinetic equivalence of bevacizumab biosimilar LY01008 with reference bevacizumab(Avastin).This randomized controlled trial aimed to compare the efficacy and safety of LY01008 with Avastin in first-line treatment of Chinese patients with advanced or recurrent non-squamous non-small cell lung cancer(NSCLC).Methods:StageⅢB-ⅣNSCLC patients with evaluable lesions,good physical status,and adequate organ functions from 67 centers across China were randomized in a ratio of 1:1 to receive LY01008 or Avastin 15 mg/kg intravenously in combination with paclitaxel/carboplatin(combined treatment)for 4-6 cycles,followed by maintenance monotherapy with LY01008 until disease progression,intolerable toxicity,or death.The primary endpoint was objective response rate(ORR)in accordance with Response Evaluation Criteria in Solid Tumors(RECIST)version 1.1 confirmed by independent radiological review committees(IRRC).Secondary endpoints included disease control rate(DCR),duration of response(DoR),progression-free survival(PFS),overall survival(OS),and safety.This study was registered in Clinical Trials.gov(NCT03533127).Results:Between December 15^(th),2017,and May 15^(th),2019,a total of 649 patients were randomized to the LY01008(n=324)or Avastin(n=325)group.As of September 25th,2019 for primary endpoint analysis,589 patients received ORR evaluation,with a median number of combined treatment cycles of 5(range 1-6)andmedian duration of treatment of 3.0(range 0.0-5.1)months.ORRof responseevaluable patients in the LY01008 and Avastin groups were 48.5% and 53.0%,respectively.The stratified ORR ratio was 0.91(90%CI 0.80-1.04,within the prespecified equivalence margin of 0.75-1.33).Up to May 15^(th),2020,with a median follow-up of 13.6(range 0.8-28.4)months,no notable differences in DCR,median DoR,median PFS,median OS,and 1-year OS rate were observed between the LY01008 and Avastin groups.There were no clinically meaningful differences in safety and immunogenicity across treatment groups.Conclusions:LY01008 demonstrated similarity to Avastin in terms of efficacy and safety in Chinese patients with advanced or recurrent non-squamous NSCLC.LY01008 combined with paclitaxel/carboplatin is expected to become a new treatment option for unresectable,metastatic,LY01008 and Avastin groups.There were no clinically meaningful differences in safety and immunogenicity across treatment groups.Conclusions:LY01008 demonstrated similarity to Avastin in terms of efficacy and safety in Chinese patients with advanced or recurrent non-squamous NSCLC.LY01008 combined with paclitaxel/carboplatin is expected to become a new treatment option for unresectable,metastatic,or recurrent non-squamous NSCLC patients in the first-line setting. | Yuankai Shi Kaijian Lei Yuming Jia Bingqiang Ni Zhiyong He Minghong Bi Xicheng Wang Jianhua Shi Ming Zhou Qian Sun Guolei Wang Dongji Chen Yongqian Shu Lianke Liu Zhongliang Guo Yong Liu Junquan Yang Ke Wang Ke Xiao LinWu Tienan Yi Debin Sun Mafei Kang Tianjiang Ma Yimin Mao Jinsheng Shi Tiegang Tang Yan Wang Puyuan Xing Dongqing Lv Wangjun Liao Zhiguo Luo Bin Wang Xiaohong Wu Xiaoli Zhu Shuhua Han Qisen Guo Rongyu Liu Zhiwei Lu Jianyong Zhang Jian Fang Changlu Hu Yinghua Ji Guolong Liu Hong Lu Dedong Wu Junhong Zhang Shuyang Zhu Zheng Liu Wensheng Qiu Feng Ye Yan Yu Yanqiu Zhao Qinhong Zheng Jun Chen Zhanyu Pan Yiping Zhang Wenjuan Lian Bo Jiang Bo Qiu Guojun Zhang Hua Zhang Yanju Chen Yuan Chen Hongbing Duan Manxiang Li Shengming Liu Lijun Ma Hongming Pan Xia Yuan Xueli Yuan Yulong Zheng Emei Gao Li Zhao Shumin Wang Can Wu | 2021 | Cancer Communications2021，41，9： | 5 |
| 3 | COVID-19-associated monocytic encephalitis(CAME):histological and proteomic evidence from autopsy显示文摘Severe neurological symptoms are associated with Coronavirus disease 2019(COVID-19).However,the morphologic features,pathological nature and their potential mechanisms in patient brains have not been revealed despite evidence of neurotropic infection.In this study,neuropathological damages and infiltrating inflammatory cells were quantitatively evaluated by immunohistochemical staining,ultrastructural examination under electron microscopy,and an image threshold method,in postmortem brains from nine critically ill COVID-19 patients and nine age-matched cadavers of healthy individuals.Differentially expressed proteins were identified by quantitative proteomic assays.Histopathological findings included neurophagocytosis,microglia nodules,satellite phenomena,extensive edema,focal hemorrhage,and infarction,as well as infiltrating mononuclear cells.Immunostaining of COVID-19 brains revealed extensive activation of both microglia and astrocytes,severe damage of the blood-brain barrier(BBB)and various degrees of perivascular infiltration by predominantly CD14+/CD16+/CD141+/CCR7+/CD11c+monocytes and occasionally CD4+/CD8+T lymphocytes.Quantitative proteomic assays combined with bioinformatics analysis identified upregulated proteins predominantly involved in immune responses,autophagy and cellular metabolism in COVID-19 patient brains compared with control brains.Proteins involved in brain development,neuroprotection,and extracellular matrix proteins of the basement membrane were downregulated,potentially caused by the activation of transforming growth factorβreceptor and vascular endothelial growth factor signaling pathways.Thus,our results define histopathological and molecular profiles of COVID-19-associated monocytic encephalitis(CAME)and suggest potential therapeutic targets. | Pei-Pei Zhang Zhi-Cheng He Xiao-Hong Yao Rui Tang Jie Ma Tao Luo Chuhong Zhu Tian-Ran Li Xindong Liu Dingyu Zhang Shuyang Zhang Yi-Fang Ping Ling Leng Xiu-Wu Bian | 2023 | Signal Transduction and Targeted Therapy2023，8，2： | 3 |
| 4 | Gene duplication drove the loss of awn in sorghum.显示文摘Loss of the awn in some cereals including sorghum is a key transition during cereal domestication or improvement that has facilitated grain harvest and storage.The genetic basis for the loss of awn in sorghum during domestication or improvement remains unknown.Here,we identified a transcription factor gene awn1 encoding an ALOG domain,which is responsible for awn loss during sorghum domestication or improvement.awn1 arose from a gene duplication from chromosome 10 that translocated to chromosome 3,recruiting a new promoter from the neighbouring intergenic region filled with'noncoding DNA',and recreating the first exon and intron.The awn1 acquires high expr`ession after duplication and represses the elongation of awns in domesticated sorghum.Comparative mapping revealed a high collinearity at awn1 paralog locus on chromosome 10 across cereals and awn growth and development was successfully reactivated on the rice spikelet by inactivating rice awn1 orthologue.Further RNA-seq and DAP-seq revealed that as a transcription repressor,AWN1 directly bound to the motif in the regulatory regions from three MADS genes related to flower development and two genes DL and LKS2 for the development of awn,downregulated the expressions of these genes,and then repressed the elongation of awn.The preexistence of regulatory elements in the neighbouring intergenic region of awn1 before domestication signified that noncoding DNA may serve as a treasure trove for evolution during adaptation to a changing world.Our results supported that gene duplication can promptly drive the evolution of gene regulatory network. | Leina Zhou Can Zhu Xiaojian Fang Hangqin Liu Shuyang Zhong Yan Li Jiacheng Liu Yang Song Xing Jian Zhongwei Lin | 2021 | Molecular Plant2021，14，11： | 2 |
| 5 | A plasma microRNA panel for early detection of colorectal cancer显示文摘 | Shuyang Wang Jianbin Xiang Zhaoyong Li Shaohua Lu Jie Hu Xue Gao Lei Yu Lei Wang Jiping Wang Ying Wu Zongyou Chen Hongguang Zhu | 2015 | Int. J. Cancer2015，，1： | 1 |
| 6 | Empirical Effective Interaction in 22MeV Proton Scattering显示文摘Inelastic differential cross sections of 22 MeV proton scattering for 5 excited states of 28Si have been measured from 10°to 115°.Empirical effective interactions were extracted within the frame of DWBA from the normal parity state data which transition densities were known from electron scattering.The fitting is good except for the forward angle of 4^(+)_(1) and 0^(+)_(2) states.The results are discussed. | AN Zhu CHEN Quan DING Dazhao SHI Zongren WAN Shuyang GAO Keyang | 1994 | Chinese Physics Letters1994，11，7： | 0 |
| 7 | Non-Ischemic, Non-Hypoxic Myocardial Injury, and Long-Term Mortality in Patients with Coronavirus Disease 2019: A Retrospective Cohort Study显示文摘Objective:Cardiac damage is commonly reported in patients with coronavirus disease 2019(COVID-19)but its prevalence and impact on the long-term survival of patients remain uncertain.This study aimed to explore the prevalence of myocardial injury and assess its prognostic value in patients with COVID-19.Methods:A single-center,retrospective cohort study was performed at the Affiliated Hospital of Jianghan University.Data from 766 patients with confirmed COVID-19 who were hospitalized from December 27,2019 to April 25,2020 were collected.Demographic,clinical,laboratory,electrocardiogram,treatment data and all-cause mortality during follow-up were collected and analyzed.Results:Of the 766 patients with moderate to critically ill COVID-19,86(11.2%)died after a mean follow-up of 72.8 days.Myocardial injury occurred in 94(12.3%)patients.The mortality rate was 64.9%(61/94)and 3.7%(25/672)in patients with and without myocardial injury,respectively.Cox regression showed that myocardial injury was an independent risk factor for mortality(hazard ratio:8.76,95%confidence interval:4.76–16.11,P<0.001).Of the 90 patients with myocardial injury with electrocardiogram results,sinus tachycardia was present in 29,bundle branch block in 26,low voltage in 10,and abnormal T-wave in 53.Conclusions:COVID-19 not only involves pneumonia but also cardiac damage.Myocardial injury is a common complication and an independent risk factor for mortality in COVID-19 patients. | Fajiu Li Xijie Zhu Ziyang Zhu Yinjian Yang Zhuang Tian Duolao Wang Shi Chen Xiaoyan Gao Yalin Xu Bo Zhang Wei Yu Min Liu Xiqi Xu Chenghong Li Shuyang Zhang | 2022 | Cardiology Discovery2022，2，2： | 0 |
| 8 | Mean-field type forward-backward doubly stochastic differential equations and related stochastic differential games显示文摘We study a kind of partial information non-zero sum differential games of mean-field backward doubly stochastic differential equations,in which the coefficient contains not only the state process but also its marginal distribution,and the cost functional is also of mean-field type.It is required that the control is adapted to a sub-filtration of the filtration generated by the underlying Brownian motions.We establish a necessary condition in the form of maximum principle and a verification theorem,which is a sufficient condition for Nash equilibrium point.We use the theoretical results to deal with a partial information linear-quadratic(LQ)game,and obtain the unique Nash equilibrium point for our LQ game problem by virtue of the unique solvability of mean-field forward-backward doubly stochastic differential equation. | Qingfeng ZHU Lijiao SU Fuguo LIU Yufeng SHI Yong’ao SHEN Shuyang WANG | 2020 | Frontiers of Mathematics in China2020，15，6： | 0 |
| 9 | Key Issues and Compiling Approach of Accessibility Design Standards:A Case Study of Shenzhen显示文摘Both practice and research of accessible design in China are still in the initial stage,while the regulations and standardization of accessibility is taking shape.Under the framework of engineering standards based on instrumental rationality,to present the core value of humanism and to define the limitations of universal design in the standards are the key issues worth discussing continuously.By comparing the standards of China and abroad,this paper attempts to clarify the approaches for compiling the Accessibility Design Standards for Shenzhen.At the same time,it provides materials for discussion by showing the difficulties and assumptions during the compilation. | Liu Fang Zhu Yanmei Li Shuyang Ni Zhenyu | 2021 | China City Planning Review2021，30，3： | 0 |
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