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| 1 | Development and utilization of a new chemically-induced soybean library with a high mutation density显示文摘Mutagenized populations have provided important materials for introducing variation and identifying gene function in plants. In this study, an ethyl methanesulfonate(EMS)-induced soybean(Glycine max) population,consisting of 21,600 independent M_2 lines, was developed.Over 1,000 M_(4(5))families, with diverse abnormal phenotypes for seed composition, seed shape, plant morphology and maturity that are stably expressed across different environments and generations were identified. Phenotypic analysis of the population led to the identification of a yellow pigmentation mutant, gyl, that displayed significantly decreased chlorophyll(Chl) content and abnormal chloroplast development. Sequence analysis showed that gyl is allelic to Minn Gold, where a different single nucleotide polymorphism variation in the Mg-chelatase subunit gene(ChlI1a) results in golden yellow leaves. A cleaved amplified polymorphic sequence marker was developed and may be applied to marker-assisted selection for the golden yellow phenotype in soybean breeding. We show that the newly developed soybean EMS mutant population has potential for functional genomics research and genetic improvement in soybean. | Zhongfeng Li Lingxue Jiang Yansong Ma Zhongyan Wei Huilong Hong Zhangxiong Liu Jinhui Lei Ying Liu Rongxia Guan Yong Guo Longguo Jin Lijuan Zhang Yinghui Li Yulong Ren Wei He Ming Liu Nang Myint Phyu Sin Htwe Lin Liu Bingfu Guo Jian Song Bing Tan Guifeng Liu Maiquan Li Xianli Zhang Bo Liu Xuehui Shi Sining Han Sunan Hua Fulai Zhou Lili Yu Yanfei Li Shuang Wang Jun Wang Ruzhen Chang Lijuan Qiu | 2017 | Journal of Integrative Plant Biology2017，59，1： | 6 |
| 2 | A Virulent PEDV Strain FJzz1 with Genomic Mutations and Deletions at the High Passage Level Was Attenuated in Piglets via Serial Passage In Vitro显示文摘Highly virulent porcine epidemic diarrhea virus(PEDV)strains re-emerged and circulated in China at the end of 2010,causing significant economic losses in the pork industry worldwide.To understand the genetic dynamics of PEDV during its passage in vitro,the PEDV G2 strain FJzzl was serially propagated in Vero cells for up to 200 passages.The susceptibility and adaptability of the FJzzl strain increased gradually as it was serially passaged in vitro.Sequence analysis revealed that amino acid(aa)changes were mainly concentrated in the S glycoprotein,which accounted for 72.22%-85.71%of all aa changes.A continuous aa deletion(^(55)I^(56)G^(57)E→^(55)K^(56)Δ^(57)Δ)occurred in the N-terminal domain of S1(Sl-NTD).To examine how the aa changes affected its virulence,FJzzl-F20 and FJzzl-F200 were selected to simultaneously evaluate their pathogenicity in suckling piglets.All the piglets in the FJzzl-F20-infected group showed typical diarrhea at 24 h postinfection,and the piglets died successively by 48 h postinfection.However,the clinical signs of the piglets in the FJzzl-F200-infected group were significantly weaker,and no deaths occurred.The FJzzl-F200-infected group also showed a lower level of fecal viral shedding and lower viral loads in the intestinal tissues,and no obvious histopathological lesions.TypeⅠandⅢinterferon were induced in the FJzzl-F200 infection group,together with pro-inflammatory cytokines,such as TNF-α,IL-1βand IL-8.These results indicate that the identified genetic changes may contribute to the attenuation of FJzzl strain,and the attenuated FJzzl-F200 may have the potential for developing PEDV live-attenuated vaccines. | Pengfei Chen Xiongwei Zhao Shuting Zhou Tianxing Zhou Xiangmei Tan Xia Wu Wu Tong Fei Gao Lingxue Yu Yifeng Jiang Hai Yu Zhibiao Yang Guangzhi Tong Yanjun Zhou | 2021 | Virologica Sinica2021，36，5： | 4 |
| 3 | Spatio-temporal Pattern and Spatial Heterogeneity of Ecotones Based oh Land Use Types of Southeastern Da Hinggan Mountains in China显示文摘Ecotones have received great attention due to its critical function in energy flux, species harbor, global carbon sequestration, and land-atmosphere interaction. This study investigated land use pattern and spatial heterogeneity of the ecotones among agricultural land, forest land, and grassland of the southeastern Da Hinggan Mountains in the northeastern China. The change of these delineated ecotones under different slopes and aridity conditions was analyzed by two landscape indices, edge density(ED) and core area percentage of landscape(CPL), to explore the inter-linkage between spatial structure of ecotones and socioeconomic development and land management. Specifically, the ecotones such as agriculture-forest(AF) ecotone, forest-grassland(FG) ecotone, and agriculture-forestgrassland(AFG) ecotone moved from the arid southeast to the humid northwest. The flat area with small slope is more edge-fragmented than the steep area since the ED decreases as the slope increases. The AF ecotone mostly found in the humid region is moving to more humid areas while the agriculture-grassland(AG) ecotone mostly found in the dry region is moving towards the drier region. | YU Lingxue ZHANG Shuwen LIU Tingxiang TANG Junmei BU Kun YANG Jiuchun | 2015 | Chinese Geographical Science2015，25，2： | 4 |
| 4 | China's Wetland Databases Based on Remote Sensing Technology显示文摘Wetland databases can provide the basic data that necessary for the protection and management of wetlands. A large number of wetland databases have been established in the world as well as in China. In this paper, we review China's wetland databases based on remote sensing(RS) technology after introducing the background theory to the application of RS technology in wetland surveys. A key conclusion is that China's wetland databases are far from sufficient in fulfilling protection and management needs. Our recommendations focus on the use of the hyper-spectral imagery, microwave data, multi-temporal images, and automatic classifications in order to improve the accuracy and efficiency of wetland inventory. Further, attention should also be paid to detect major biophysical features of wetlands and build wetland databases in years after the 1980 s in China. Considering that great gap exists between RS experts and wetland experts, further cooperation between wetland scientists and RS scientists are needed to promote the application of RS in the foundation of wetland databases. | YAN Fengqin LIU Xingtu CHEN Jing YU Lingxue YANG Chaobin CHANG Liping YANG Jiuchun ZHANG Shuwen | 2017 | Chinese Geographical Science2017，27，3： | 4 |
| 5 | Genetic characterization and pathogenicity of a reassortant Eurasian avian-like H1N1 swine influenza virus containing an internal gene cassette from 2009 pandemic H1N1 virus显示文摘Dear editor,Swine influenza virus(SIV)is a member of the Orthomyxoviridae family,influenza A virus genus,which can cause the swine influenza—an acute and highly contagious respiratory disease in pigs(Brown,2000;Kothalawala et al.,2006).The SIV was first observed in 1918 in the United States and the progenitor of the SIV was the H1N1 influenza virus which caused the Spanish influenza pandemic of 1918(Shope,1931). | Shuaiyong Wang Manzhu Wang Lingxue Yu Juan Wang Jiecong Yan Xinli Rong Yanjun Zhou Tongling Shan Wu Tong Guoxin Li Hao Zheng Guangzhi Tong Hai Yu | 2022 | Virologica Sinica2022，37，4： | 0 |
| 6 | The Novel PRRSV Strain HBap4-2018 with a Unique Recombinant Pattern Is Highly Pathogenic to Piglets显示文摘Currently, various porcine reproductive and respiratory syndrome virus(PRRSV) variants emerged worldwide with different genetic characteristics and pathogenicity, increasing the difficulty of PRRS control. In this study, a PRRSV strain named HBap4-2018 was isolated from swine herds suffering severe respiratory disease with high morbidity in Hebei Province of China in 2018. The genome of HBap4-2018 is 15,003 nucleotides in length, and compared with NADC30-like PRRSV, nsp2 of HBap4-2018 has an additional continuous deletion of five amino acids. Phylogenetic analysis based on complete genome and ORF5 showed that HBap4-2018 belonged to lineage 8 of PRRSV-2, which was characterized by highly variable genome. However, HBap4-2018 was classified into lineage 1 based on phylogenetic analysis of nsp2,sharing higher amino acid homology(85.3%–85.5%) with NADC30-like PRRSV. Further analysis suggested that HBap4-2018 was a novel natural recombinant PRRSV with three recombinant fragments in the genome, of which highly pathogenic PRRSV(HP-PRRSV) served as the major parental strains, while NADC30-like PRRSV served as the minor parental strains. Five recombination break points were identified in nsp2, nsp3, nsp5, nsp9 and ORF6, respectively,presenting a novel recombinant pattern in the genome. Piglets inoculated with HBap4-2018 presented typical clinical signs with a mortality rate of 60%. High levels of viremia and obvious macroscopic and histopathological lesions in the lungs were observed, revealing the high pathogenicity of HBap4-2018 in piglets. | Pengfei Chen Xiangmei Tan Mengqin Lao Xia Wu Xiongwei Zhao Shuting Zhou Jiarong Yu Junrui Zhu Lingxue Yu Wu Tong Fei Gao Hai Yu Changlong Liu Yifeng Jiang Guangzhi Tong Yanjun Zhou | 2021 | Virologica Sinica2021，36，6： | 0 |
| 7 | Climate Change Impacts on Agroecosystems in China:Processes,Mechanisms and Prospects显示文摘Building a more resilient response system to climate change for sustainable development and reducing uncertainty in China’s food markets,requires access to historical research gaps and mapping future research progress for decision making.However,the lack of quantitative and objective analyses to ensure the stability and development of agroecosystems increases the complexity of agro-climatic mechanisms,which leads to uncertainty and undesirable consequences.In this paper,we review the characteristics of climate change in China(1951–2020),reveal the mechanisms of agroecosystem structure in response to climate,and identify challenges and opportunities for future efforts in the context of research progress.The aim is to improve the scientific validity and relevance of future research by clarifying agro-climatic response mechanisms.The results show that surface temperature,precipitation,and frequency of extreme weather events have increased to varying degrees in major agricultural regions of China in 1951–2020.And they have strong geographic variation,which has resulted in droughts in the north and floods in the south.Moreover,climate change has complicated the mechanisms of soil moisture,Net Primary Productivity(NPP),soil carbon pool,and crop pest structure in agroecosystems.This lends to a reduction in soil water holding capacity,NPP,soil carbon content,and the number of natural enemies of diseases and insects,which in turn affects crop yields.However,human interventions can mitigate the deterioration of these factors.We have also realized that the methodology and theory of historical research poses a great challenge to future agroecosystem.Historical and projected climate trends identified current gaps in interdisciplinary integration and multidisciplinary research required to manage diverse spatio-temporal climate change impacts on agroecosystems.Future efforts should highlight integrated management and decision making,multidisciplinary big data coupling,and numerical simulations to ensure sustainable agricultural development,ecological security,and food security in China. | BAO Lun YU Lingxue LI Ying YAN Fengqin LYNE Vincent REN Chunying | 2023 | Chinese Geographical Science2023，33，4： | 0 |
| 8 | Lamellar-stacked cobalt-based nanopiles integrated with nitrogen/sulfur co-doped graphene as a bifunctional electrocatalyst for ultralong-term zinc-air batteries显示文摘Sluggish oxygen evolution reaction(OER)and oxygen reduction reaction(ORR)kinetics inevitably impede the practical performance of rechargeable zinc-air batteries.Thus,combing the structural designability of transition metal-based electrocatalysts with anionic regulation is highly desired.Herein,mesoporous lamellar-stacked cobalt-based nanopiles with surface-sulfurization modification are elaborately designed and integrated with N/S co-doped graphene to build a robust OER/ORR bifunctional electrocatalyst.The lamellar-stacking mode of mesoporous nanosheets with abundant channels accelerates gas-liquid mass transfer,and partial-sulfurization of cobalt-based matrix surface efficiently improves the intrinsic OER activity.Meanwhile,N/S co-doped graphene further reinforces the ORR active sites while providing a stable conductive skeleton.As expected,this composite electrocatalyst delivers considerable bifunctional activity and stability,with an OER overpotential of 323 m V at 10 m A cm^(-2)and high durability.When applied in zinc-air batteries,remarkable ultralong-term stability over 4000 cycles and a maximum power density of 150.1 m W cm^(-2)are achieved.This work provides new insight into structurecomposition synergistic design of rapid-kinetics OER/ORR bifunctional electrocatalyst for nextgeneration metal-air batteries. | Lingxue Meng Wenwei Liu Yang Lu Zhenyi Liang Ting He Jinying Li Haoxiong Nan Shengxu Luo Jia Yu | 2023 | Journal of Energy Chemistry2023，，6： | 0 |
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