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| 1 | The Epitope Study on the SARS-CoV Nucleocapsid Protein显示文摘The nucleocapsid protein (N protein) has been found to be an antigenic protein in a number of coronaviruses. Whether the N protein in severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is antigenic remains to be elucidated. Using Western blot and Enzyme-linked Immunosorbent Assay (ELISA),the recombinant N proteins and the synthesized peptides derived from the N protein were screened in sera from SARS patients. All patient sera in this study displayed strong positive immunoreactivities against the recombinant N proteins,whereas normal sera gave negative immunoresponses to these proteins, indicating that the N protein of SARS-CoV is an antigenic protein. Furthermore, the epitope sites in the N protein were determined by competition experiments, in which the recombinant proteins or the synthesized peptides competed against the SARS-CoV proteins to bind to the antibodies raised in SARS sera. One epitope site located at the C-terminus was confirmed as the most antigenic region in this protein. A detailed screening of peptide with ELISA demonstrated that the amino sequence from Codons 371 to 407 was the epitope site at the C-terminus of the N protein. Understanding of the epitope sites could be very significant for developing an effective diagnostic approach to SARS. | Shuting Li, Liang Lin, Hao Wang, Jianning Yin, Yan Ren, Zhe Zhao, Jie Wen, Cuiqi Zhou, Xumin Zhang, Xiaolei Li, Jingqiang Wang, Zhengfeng Zhou, Jinxiu Liu, Jianmin Shao, Tingting Lei, Jianqiu Fang, Ningzhi Xu, and Siqi LiuBeijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China & Beijing Proteomics Institute, Beijing 101300, China | 2003 | Genomics, Proteomics & Bioinformatics2003，1，3： | 9 |
| 2 | Two selenium tolerant Lysinibacillus sp. strains are capable of reducing selenite to elemental Se efficiently under aerobic conditions显示文摘Microbes play important roles in the transport and transformation of selenium(Se) in the environment, thereby influencing plant resistance to Se and Se accumulation in plant. The objectives are to characterize the bacteria with high Se tolerance and reduction capacity and explore the significance of microbial origins on their Se tolerance, reduction rate and efficiency. Two bacterial strains were isolated from a naturally occurred Se-rich soil at tea orchard in southern Anhui Province, China. The reduction kinetics of selenite was investigated and the reducing product was characterized using scanning electron microscopy and transmission electron microscopy-energy dispersive spectroscopy. The bacteria were identified as Lysinibacillus xylanilyticus and Lysinibacillus macrolides,respectively, using morphological, physiological and molecular methods. The results showed that the minimal inhibitory concentrations(MICs) of selenite for L. xylanilyticus and L. macrolides were 120 and 220 mmol/L, respectively, while MICs of selenate for L.xylanilyticus and L. macrolides were 800 and 700 mmol/L, respectively. Both strains aerobically reduced selenite with an initial concentration of 1.0 mmol/L to elemental Se nanoparticles(SeNPs) completely within 36 hr. Biogenic SeNPs were observed both inside and outside the cells suggesting either an intra-or extracellular reduction process. Our study implied that the microbes from Se-rich environments were more tolerant to Se and generally quicker and more efficient than those from Se-free habitats in the reduction of Se oxyanions. The bacterial strains with high Se reduction capacity and the biological synthesized Se NPs would have potential applications in agriculture, food, environment and medicine. | Ju Zhang Yue Wang Zongyuan Shao Jing Li Shuting Zan Shoubiao Zhou Ruyi Yang | 2019 | Journal of Environmental Sciences2019，31，3： | 7 |
| 3 | MiR-29a/b/c regulate human circadian gene hPER1 expression by targeting its 3/UTR显示文摘在哺乳动物的几必要生物进步被生理节奏的节奏调整。不过分子的机制 ofoscillating 这些生理节奏的节奏被揭开了,生理节奏的基因的特定的功能不是很, clear.It 被报导了那由 microRNA 将生理节奏的基因击倒是有用策略探索 circadianrhythms 的功能。在这研究,通过屏蔽途径的前面的生物信息学,我们作为有势力 inhibitorsfor 识别了 miR-29a/b/c 人的生理节奏的基因 hPER1。我们进一步发现 miR-29a/b/c 能直接在人的 A549 房间在 mRNA 和蛋白质表示层次指向 hPER1 3untranslated 区域(UTR ) 和下面调整 hPER1。因此,我们的调查结果建议 hPER1 的表示被 miR-29a/b/c 调整,它可以也为 hPER1 的功能提供新线索。 | Xiyan Zhao Xueqiang Zhu Shuting Cheng Yizhou Xie Zhengrong Wang Yanyou Liu Zhou Jiang Jing Xiao Huiling Guo Yuhui Wang | 2014 | Acta Biochimica et Biophysica Sinica2014，46，4： | 7 |
| 4 | A Virulent PEDV Strain FJzz1 with Genomic Mutations and Deletions at the High Passage Level Was Attenuated in Piglets via Serial Passage In Vitro显示文摘Highly virulent porcine epidemic diarrhea virus(PEDV)strains re-emerged and circulated in China at the end of 2010,causing significant economic losses in the pork industry worldwide.To understand the genetic dynamics of PEDV during its passage in vitro,the PEDV G2 strain FJzzl was serially propagated in Vero cells for up to 200 passages.The susceptibility and adaptability of the FJzzl strain increased gradually as it was serially passaged in vitro.Sequence analysis revealed that amino acid(aa)changes were mainly concentrated in the S glycoprotein,which accounted for 72.22%-85.71%of all aa changes.A continuous aa deletion(^(55)I^(56)G^(57)E→^(55)K^(56)Δ^(57)Δ)occurred in the N-terminal domain of S1(Sl-NTD).To examine how the aa changes affected its virulence,FJzzl-F20 and FJzzl-F200 were selected to simultaneously evaluate their pathogenicity in suckling piglets.All the piglets in the FJzzl-F20-infected group showed typical diarrhea at 24 h postinfection,and the piglets died successively by 48 h postinfection.However,the clinical signs of the piglets in the FJzzl-F200-infected group were significantly weaker,and no deaths occurred.The FJzzl-F200-infected group also showed a lower level of fecal viral shedding and lower viral loads in the intestinal tissues,and no obvious histopathological lesions.TypeⅠandⅢinterferon were induced in the FJzzl-F200 infection group,together with pro-inflammatory cytokines,such as TNF-α,IL-1βand IL-8.These results indicate that the identified genetic changes may contribute to the attenuation of FJzzl strain,and the attenuated FJzzl-F200 may have the potential for developing PEDV live-attenuated vaccines. | Pengfei Chen Xiongwei Zhao Shuting Zhou Tianxing Zhou Xiangmei Tan Xia Wu Wu Tong Fei Gao Lingxue Yu Yifeng Jiang Hai Yu Zhibiao Yang Guangzhi Tong Yanjun Zhou | 2021 | Virologica Sinica2021，36，5： | 4 |
| 5 | Quantized Phase Step Technique Based on Different Frequency Signals and Its Characteristics显示文摘 | DU Baoqiang DONG Shaofeng ZHOU Wei WANG Yanfeng GUO Shuting | 2013 | Chinese Journal of Electronics2013，22，3： | 4 |
| 6 | A Distributed Approach of Big Data Mining for Financial Fraud Detection in a Supply Chain显示文摘Supply Chain Finance(SCF)is important for improving the effectiveness of supply chain capital operations and reducing the overall management cost of a supply chain.In recent years,with the deep integration of supply chain and Internet,Big Data,Artificial Intelligence,Internet of Things,Blockchain,etc.,the efficiency of supply chain financial services can be greatly promoted through building more customized risk pricing models and conducting more rigorous investment decision-making processes.However,with the rapid development of new technologies,the SCF data has been massively increased and new financial fraud behaviors or patterns are becoming more covertly scattered among normal ones.The lack of enough capability to handle the big data volumes and mitigate the financial frauds may lead to huge losses in supply chains.In this article,a distributed approach of big data mining is proposed for financial fraud detection in a supply chain,which implements the distributed deep learning model of Convolutional Neural Network(CNN)on big data infrastructure of Apache Spark and Hadoop to speed up the processing of the large dataset in parallel and reduce the processing time significantly.By training and testing on the continually updated SCF dataset,the approach can intelligently and automatically classify the massive data samples and discover the fraudulent financing behaviors,so as to enhance the financial fraud detection with high precision and recall rates,and reduce the losses of frauds in a supply chain. | Hangjun Zhou Guang Sun Sha Fu Xiaoping Fan Wangdong Jiang Shuting Hu Lingjiao Li | 2020 | Computers, Materials & Continua2020，，8： | 3 |
| 7 | Historical Evolution of Mariculture in China During Past 40 Years and Its Impacts on Eco-environment显示文摘The rapid development of mariculture in China in recent decades has satisfied people's demand of seafood, and has made a great contribution to economic development. However, mariculture has also caused some negative impacts on the eco-environment. By statistically analyzing national data(as well as data for four main provinces) regarding the mariculture area and cultivation species in China since reform and opening up, the historical evolution of mariculture during the past 40 years was analyzed, the driving factors related to policy, market and technology innovation were discussed, and the potential impacts of mariculture on the coastal eco-environment were also illustrated. The statistical results indicated that the maricultural area increased radically during the past 40 years, from 1979(116.47 × 10~3 ha) to 2012(2205.65 × 10~3 ha), with an increase of nearly 20-fold. Shandong Province represented the fastest-growing region, with an increase of 31-fold. Moreover, the cultivated species had gradually become diversified. Initially, shellfish and algae were major species, and then it developed to various species including fish, and currently shellfish and crustaceans were the major species. The development of mariculture was driven by government policies, market economy and technology innovation. Rapid development of mariculture also caused significantly adverse impacts on the eco-environment of the coastal regions. For example, the sea reclamation for mariculture decreased the area and function of coastal wetlands and the contaminants originating from mariculture destroyed the coastal aquatic environment. Fortunately, the implementation of ‘13 th Five-Year Fisheries Planning' is expected to help both improve the seafood quality and reduce the contamination in coastal aquatic environment. This current study will provide reference for management and structure adjustment of mariculture in the future. | LIANG Yinxiu CHENG Xianwei ZHU Hui Brian SHUTES YAN Baixing ZHOU Qingwei YU Xiangfei | 2018 | Chinese Geographical Science2018，28，3： | 3 |
| 8 | Genomic evidence of lung carcinogenesis associated with coal smoke in Xuanwei area, China显示文摘Xuanwei area, in southwestern China,harbors the highest female lung cancer rate in the country(Supplementary Table 1) [1]. Epidemiological studies have shown that lung cancer incidence in Xuanwei area is associated with the use of different types of local coal for household cooking and heating [2]. The genomic landscape of Xuanwei female adenocarcinoma(XWFA), the most distinctive feature of lung cancer in Xuanwei, has yet to be elucidated systematically. Here,we provide the fundamental resource of genomic datasets for further exploration of XWFA molecular mechanisms and development of targetable therapy. | Honglei Zhang Chao Liu Li Li Xu Feng Qing Wang Jihua Li Shaobin Xu Shuting Wang Qianlu Yang Zhenghai Shen Jinhua Su Xiaosan Su Ruifen Sun Xuhong Zhou Junliang Wang Yongchun Zhou Baowei Jiao Wanbao Ding Xianbao Cao Yue Wang Yunchao Huang Lianhua Ye | 2021 | National Science Review2021，8，12： | 2 |
| 9 | RNA Methylome Reveals the m^(6)A-mediated Regulation of Flavor Metabolites in Tea Leaves under Solar-withering显示文摘The epitranscriptomic mark N6-methyladenosine(m^(6)A),which is the predominant internal modification in RNA,is important for plant responses to diverse stresses.Multiple environmental stresses caused by the tea-withering process can greatly influence the accumulation of specialized metabolites and the formation of tea flavor.However,the effects of the m^(6)A-mediated regulatory mechanism on flavor-related metabolic pathways in tea leaves remain relatively uncharacterized.We performed an integrated RNA methylome and transcriptome analysis to explore the m^(6)Amediated regulatory mechanism and its effects on flavonoid and terpenoid metabolism in tea(Camellia sinensis)leaves under solar-withering conditions.Dynamic changes in global m^(6)A level in tea leaves were mainly controlled by two m^(6)A erasers(CsALKBH4A and CsALKBH4B)during solar-withering treatments.Differentially methylated peak-associated genes following solarwithering treatments with different shading rates were assigned to terpenoid biosynthesis and spliceosome pathways.Further analyses indicated that CsALKBH4-driven RNA demethylation can directly affect the accumulation of volatile terpenoids by mediating the stability and abundance of terpenoid biosynthesis-related transcripts and also indirectly influence the flavonoid,catechin,and theaflavin contents by triggering alternative splicing-mediated regulation.Our findings revealed a novel layer of epitranscriptomic gene regulation in tea flavor-related metabolic pathways and established a link between the m^(6)A-mediated regulatory mechanism and the formation of tea flavor under solar-withering conditions. | Chen Zhu Shuting Zhang Chengzhe Zhou Caiyun Tian Biying Shi Kai Xu Linjie Huang Yun Sun Yuling Lin Zhongxiong Lai Yuqiong Guo | 2023 | Genomics, Proteomics & Bioinformatics2023，21，4： | 2 |
| 10 | Occurrence and impact of polychlorinated dibenzo-p-dioxins/dibenzofurans in the air and soil around a municipal solid waste incinerator显示文摘To assess the influence of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans(PCDD/Fs) on the environment in the vicinity of municipal solid waste incinerators(MSWIs), we determined the levels of PCDD/Fs in air and soil samples collected around a MSWI, which is the largest in China. The International Toxicity Equivalency Quantity(I-TEQ) concentrations of PCDD/Fs in air samples were from 0.0300 to 1.03 pg I-TEQ/m^3(0.445–13.6 pg/m^3), with an average of 0.237 pg I-TEQ/m^3, while in soil samples they ranged from 0.520 to 3.40 pg I-TEQ/g(2.41–88.7 pg/g) with an average of1.49 pg I-TEQ/g. The concentrations of PCDD/Fs in air and soil samples were comparable to other areas, and Pe CDFs were the dominant contributors, which was different from stack gas homologue patterns. Multivariate statistical analysis showed that PCDD/Fs emission from the MSWI did not directly affect the profiles of PCDD/Fs in air and soils, so that vehicles and unidentified emission sources should be considered. The daily inhalation levels of PCDD/Fs for children(0.0110 to 0.392 pg I-TEQ/(kg·day) and adults(0.00600 to 0.221 pg I-TEQ/(kg·day) near the MSWI were lower than the tolerable daily intake of 1.00 to 4.00 pg WHO-TEQ/(kg·day), but in winter the values were higher than in summer. These results can be used as basic data for assessing the risk of PCDD/Fs exposure in residents living around this MSWI, and more monitoring programs and studies should be carried out around MSWIs. | Zhiguang Zhou Yue Ren Jiazhi Chu Nan Li Sen Zhen Hu Zhao Shuang Fan Hui Zhang Pengjun Xu Li Qi Shuting Liang Bin Zhao | 2016 | Journal of Environmental Sciences2016，28，6： | 1 |
| 11 | A Strategy for Searching Antigenic Regions in the SARS-CoV Spike Protein显示文摘In the face of the worldwide threat of severe acute respiratory syndrome (SARS)to human life, some of the most urgent challenges are to develop fast and accurate analytical methods for early diagnosis of this disease as well as to create a safe anti-viral vaccine for prevention. To these ends, we investigated the antigenicity of the spike protein (S protein), a major structural protein in the SARS-coronavirus (SARS-CoV). Based upon the theoretical analysis for hydrophobicity of the S protein, 18 peptides were synthesized. Using Enzyme-Linked Immunosorbent Assay (ELISA), these peptides were screened in the sera from SARS patients. According to these results, two fragments of the S gene were amplified by PCR and cloned into pET-32a. Both S fragments were expressed in the BL-21 strain and further purified with an affinity chromatography. These recombinant S fragments were confirmed to have positive cross-reactions with SARS sera, either by Western blot or by ELISA. Our results demonstrated that the potential epitope regions were located at Codons 469-882 in the S protein, and one epitope site was located at Codons 599-620. Identification of antigenic regions in the SARS-CoV S protein may be important for the functional studies of this virus or the development of clinical diagnosis. | Yan Ren, Zhengfeng Zhou, Jinxiu Liu, Liang Lin, Shuting Li, Hao Wang, Ji Xia, Zhe Zhao, Jie Wen, Cuiqi Zhou, Jingqiang Wang, Jianning Yin, Ningzhi Xu, and Siqi LiuBeijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China & Beijing Proteomics Institute, Beijing 101300, China. | 2003 | Genomics, Proteomics & Bioinformatics2003，1，3： | 1 |
| 12 | Air-Stable Dy(III)-Macrocycle Enantiomers:From Chiral to Polar Space Group显示文摘Magnetoelectric(ME)multiferroic materials have unique advantages in low-power and high-density information storage,because they can simultaneously display ferroelectricity and ferromagnetism.However,research on how to construct air-stable highperformance ME single-molecule magnets(SMMs)is nonexistent.Herein,by introducing homochirality while reducing molecular symmetry,two doubledecker Dy(III)enantiomers adopting the polar space group P2_(1) and exhibiting excellent thermal stability were obtained.They displayed zero field SMM behavior with an anisotropy barrier(Ueff)of ca.100 cm^(−1).This work establishes a rational chemical design strategy for crystallizing SMMs in polar space groups and elucidates the direction for future research,that is,engineering small-size high-performance SMMs. | Zhenhua Zhu Chen Zhao Quan Zhou Shuting Liu Xiao-Lei Li Akseli Mansikkamäki Jinkui Tang | 2022 | CCS Chemistry2022，4，12： | 1 |
| 13 | The valuation of China’s environmental degradation from 2004 to 2017显示文摘This paper aims to evaluate the cost of environmental degradation by adopting the conventional environmental economic methodology in China from 2004 to 2017 and summarize the change in both the causes and costs of China’s environmental degradation.Results from this study revealed the following:i.The environmental degradation cost in China increased from 511 billion yuan to 1,892 billion yuan from 2004 to 2017,and its share in the GDP decreased from 3.05% to 2.23%;ii.The environmental degradation cost growth rate was lower than the GDP growth rate.The environmental degradation cost growth rate decreased sharply,by dropping from 10% in 2014 to 2% in 2017.The environmental benefits of industrial transformation have emerged;iii.The provinces of Shandong,Hebei,Jiangsu,Henan,and Guangdong had the highest environmental degradation costs.The annual average growth rate of the environmental degradation costs in Jiangsu,Guangdong,and Zhejiang were lower than their growth rate of the GDP respectively;iv.Consideration of environmental degradation cost in decision-making could contribute to the high-quality development of China. | Guoxia Ma Fei Peng Weishan Yang Gang Yan Shuting Gao Xiafei Zhou Ji Qi Dong Cao Yue Zhao Wen Pan Hongqiang Jiang Hong Jing Guangxia Dong Minxue Gao Jingbo Zhou Fang Yu Jinnan Wang | 2020 | Environmental Science and Ecotechnology2020，，1： | 1 |
| 14 | Microglial Exosome miR-7239-3p Promotes Glioma Progression by Regulating Circadian Genes显示文摘Glioma-associated microglial cells,a key component of the tumor microenvironment,play an important role in glioma progression.In this study,the mouse glioma cell line GL261 and the mouse microglia cell line BV2 were chosen.First,circadian gene expression in glioma cells co-cultured with either M1 or M2 microglia was assessed and the exosomes of M2-polarized and unpolarized BV-2 microglia were extracted.Subsequently,we labeled the exosomes with PKH67 and treated GL261 cells with them to investigate the exosome distribution.GL261 cell phenotypes and related protein expression were used to explore the role of M2 microglial exosomes in gliomas.Then a specific miR-7239-3p inhibitor was added to verify miR-7239-3p functions.Finally,the mouse subcutaneous tumorigenic model was used to verify the tumorigenic effect of M2 microglial exosomes in vivo.Our results showed that in gliomas co-cultured with M2 microglia,the expression of the BMAL1 protein was decreased(P<0.01),while the expression of the CLOCK protein was increased(P<0.05);opposite results were obtained in gliomas co-cultured with M1 microglia.After treatment with M2 microglial exosomes,the apoptosis of GL261 cells decreased(P<0.001),while the viability,proliferation,and migration of GL261 cells increased.Increased expression of N-cadherin and Vimentin,and decreased E-cadherin expression occurred upon treatment with M2 microglial exosomes.Addition of an miR-7239-3p inhibitor to M2 microglial exosomes reversed these results.In summary,we found that miR-7239-3p in the glioma microenvironment is recruited to glioma cells by exosomes and inhibits Bmal1 expression.M2 microglial exosomes promote the proliferation and migration of gliomas by regulating tumor-related protein expression and reducing apoptosis. | Xuepei Li Junwen Guan Zhou Jiang Shuting Cheng Wang Hou Junjie Yao Zhengrong Wang | 2021 | Neuroscience Bulletin2021，37，4： | 1 |
| 15 | Upregulation of CENPM facilitates lung adenocarcinoma progression via PI3K/AKT/mTOR signaling pathway显示文摘Centromere protein M(CENPM)is essential for chromosome separation during mitosis.However,its roles in lung adenocarcinoma(LUAD)progression and metastasis remain unknown.In this study,we aimed to explore the effects of CENPM on LUAD progression as well as the underlying mechanisms.We analyzed the expression of CENPM and its correlation with clinicopathological characteristics using GEO LUAD chip datasets and TCGA dataset.We further investigated the impact of CENPM on LUAD in vitro and in vivo.In silico analysis and qRT-PCR revealed that CENPM is upregulated in LUAD compared with that in normal lung tissues.Via gain/loss-of-function assays,we further found that CENPM promotes the LUAD cell cycle,cell proliferation,migration and invasion,and inhibits cell apoptosis in vitro.The in vivo study showed that loss of CENPM inhibits the growth of A549 xenografts.Furthermore,we found that CENPM can promote the phosphorylation of mTOR rather than directly affect the mTOR content.Inhibition of mTOR activity abrogates the promoting effects of CENPM on cell cycle progression,cell proliferation,migration and invasion.Taken together,these results show that CENPM plays an important role in the growth and metastasis of LUAD and may be a promising therapeutic target in LUAD. | Chao Liu Yun Wang Yuyang Dao Shuting Wang Fei Hou Zhixian Yang Pengjie Liu Juan Lv Ling Lv Gaofeng Li Youjun Zhou Zhiyong Deng | 2022 | Acta Biochimica et Biophysica Sinica2022，54，1： | 1 |
| 16 | Evaluation of antimicrobial and anticancer activities of three peptides identified from the skin secretion of Hylarana latouchii显示文摘The skins of frogs of the family Ranidae are particularly rich sources of biologically active peptides, among which antimicrobial peptides (AMPs) constitute the major portion. Some of these have attracted the interest of researchers because they possess both antimicrobial and anticancer activities. In this study, with ‘shotgun’ cloning & MS/MS fragmentation, three AMPs, homologues of family brevinin-1 (brevinin-1HL), & temporin (temporin-HLa and temporin-HLb), were discovered from the skin secretion of the broad-folded frog, Hylarana latouchii. They exhibited various degrees of antimicrobial & antibiofilm activities against test microorganisms & hemolysis on horse erythrocytes. It was found that they could induce bacteria death through disrupting cell membranes & binding to bacterial DNA. In addition, they also showed different potencies towards human cancer cell lines. The secondary structure & physicochemical properties of each peptide were investigated to preliminarily reveal their structure–activity relationships. Circular dichroism spectrometry showed that they all adopted a canonical α-helical conformation in membrane-mimetic solvents. Notably, the prepropeptide of brevinin-1HL from H. latouchii was highly identical to that of brevinin-1GHd from Hylarana guentheri, indicating a close relationship between these two species. Accordingly, this study provides candidates for the design of novel anti-infective & antineoplastic agents to fight multidrug-resistant bacteria & malignant tumors & also offers additional clues for the taxonomy of ranid frogs. | Yan Lin Tianxing Lin Ningna Cheng Shuting Wu Jiancai Huang Xiaoling Chen Tianbao Chen Mei Zhou Lei Wang Chris Shaw | 2021 | Acta Biochimica et Biophysica Sinica2021，53，11： | 0 |
| 17 | Allergen degradation of bee pollen by lactic acid bacteria fermentation and its alleviatory effects on allergic reactions in BALB/c mice显示文摘Food allergy as a global health problem threatens food industry.Bee pollen(BP)is a typical food with allergenic potentials,although it performs various nutritional/pharmacological functions to humans.In this study,lactic acid bacteria(LAB)were used to ferment Brassica napus BP for alleviating its allergenicity.Four novel allergens(glutaredoxin,oleosin-B2,catalase and lipase)were identified with significant decreases in LAB-fermented BP(FBP)than natural BP by proteomics.Meanwhile,metabolomics analysis showed significant increases of 28 characteristic oligopeptides and amino acids in FBP versus BP,indicating the degradation of LAB on allergens.Moreover,FBP showed alleviatory effects in BALB/c mice,which relieved pathological symptoms and lowered production of allergic mediators.Microbial high-throughput sequencing analysis showed that FBP could regulate gut microbiota and metabolism to strengthen immunity,which were closely correlated with the alleviation of allergic reactivity.These findings could contribute to the development and utilization of hypoallergenic BP products. | Shuting Yin Qiangqiang Li Yuxiao Tao Enning Zhou Kun Wang Wanwen Chen Xiangxin Li Liming Wu | 2024 | Food Science and Human Wellness2024，13，1： | 0 |
| 18 | Genetic structure of the oriental white stork (Ciconia boyciana): implications for a breeding colony in a non-breeding area显示文摘The oriental white stork (Ciconia boyciana) is a threatened species, and their numbers are still in decline due tohabitat loss and poaching. China is a breeding and main wintering area for this animal and in recent years someindividuals have been found breeding in wintering areas and at some stopover sites. These new breeding coloniesare an exciting sign, however, little is understood of the genetic structure of this species. Based on the analysis ofa 463-bp mitochondrial DNA (mtDNA) control region, we investigated the genetic structure and genetic diversity of66 wild oriental white storks from a Chinese population. We analyzed the sequences of 66 storks obtained in thisstudy and the data of 17 storks from a Japanese population. Thirty-seven different haplotypes were detected amongthe 83 samples. An analysis of molecular variance showed a significant population subdivision between the twopopulations (FST = 0.316, P < 0.05). However, the phylogenetic analysis revealed that the samples from the differentpopulations did not form separate clusters and that there were genetic exchanges between the two populations.Compared with the Japanese population, the Chinese population had a relatively higher genetic diversity with ahaplotype diversity (h ± SD) of 0.953 ± 0.013 and a nucleotide diversity (π ± SD) of 0.013 ± 0.007. The high haplotypediversity and low nucleotide diversity indicate that this population might be in a rapidly increasing period from asmall effective population. A neighbor-joining tree analysis indicated that genetic exchange had occurred betweenthe newly arisen southern breeding colony and the northern breeding colony wintering in the middle and lowerYangtze River floodplain. These results have important implications for the conservation of the oriental white storkpopulation in China. | Shuting ZAN Lizhi ZHOU Hao JIANG Baowei ZHANG Zhi’an WU Yinxu HOU | 2008 | Integrative Zoology2008，3，3： | 0 |
| 19 | A reflective display based on the electro-microfluidic assembly of particles within suppressed water-in-oil droplet array显示文摘Reflective displays have stimulated considerable interest because of their friendly readability and low energy consumption.Herein,we develop a reflective display technique via an electro-microfluidic assembly of particles(eMAP)strategy whereby colored particles assemble into annular and planar structures inside a dyed water droplet to create'open'and'closed'states of a display pixel.Water-in-oil droplets are compressed within microwells to form a pixel array.The particles dispersed in droplets are driven by deformation-strengthened dielectrophoretic force to achieve fast and reversible motion and assemble into multiple structures.This eMAP based device can display designed information in three primary colors with≥170°viewing angle,~0.14 s switching time,and bistability with an optimized material system.This proposed technique demonstrates the basis of a high-performance and energy-saving reflective display,and the display speed and color quality could be further improved by structure and material optimization;exhibiting a potential reflective display technology. | Shitao Shen Haoqiang Feng Yueming Deng Shuting Xie Zichuan Yi Mingliang Jin Guofu Zhou Paul Mulvaney Lingling Shui | 2023 | Light(Science & Applications)2023，12，12： | 0 |
| 20 | KIAA1199 induces advanced biological behavior and development of ovarian cancer through activation of the IL-6/STAT3 pathway显示文摘Recently,abnormal expression of KIAA1199 has been detected in Epithelial Ovarian Cancer(EOC).However,the underlined anti-ovarian cancer mechanism of KIAA1199 remains to be enlightened.In our study,we performed to elucidate the effects of KIAA1199 on the advanced biological behavior of EOC cells through activation of the IL-6/STAT3 pathway.Confirmed by immunohistochemistry,KIAA1199 was highly expressed in ovarian borderline and malignant epithelial tumors.A retrospective analysis found that EOC patients with low expression of KIAA1199 had a significantly higher 5-year survival rate than those with high expression.Mechanistically,IL-6 was used to stimulate EOC cells,and the expression of KIAA1199,STAT3 and p-STAT3 increased after IL-6 stimulation.These results could show that KIAA1199 is transcriptionally activated by IL6/STAT3 pathway,thereby accelerating the deterioration of EOC.KIAA1199 could also be used as a poor prognosis factor and potential target in treatment. | SHUTING GU JINGYI QIN SAINAN GAO ZHEN WANG QI MENG YAN LI BING LU SONGLIN ZHOU YUNZHAO XU | 2022 | BIOCELL2022，46，3： | 0 |
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