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| 1 | Strengths, weaknesses, opportunities and threats (SWOT) analysis of reinitiation into methamphetamine abusers: qualitative findings from an exploration of methamphetamine abusers in Shanghai, China显示文摘Background Despite high relapse rate among methamphetamine (MA) abusers, there still have been little empirical data to date detecting the risk factors related to craving and relapse from the perspective of MA abusers. Therefore, the aim of this study was to use qualitative research methods exploring the strengths, weaknesses, opportunities and threat factors that could facilitate or impede individuals' abstinence in a real-life context from the perspectives of MA abusers. Aim To use qualitative research methods exploring the strengths, weaknesses, opportunities and threat factors relating to individuals' abstinence in a real-life context. Method Semistructured interviews were conducted with 32 MA abusers recruited in Shanghai guided by open-ended questions on narrating the real-life catalysts and inhibitors related to craving, initiation, relapse and abstinence. All data were transcribed verbatim and analysed using a strengths, weaknesses, opportunities and threats (SWOT) analysis. Results The results of the SWOT analysis revealed that contextual factors including peer influence, prevalence and availability of MA, familiar venue of MA use, discrimination, sexual behaviours, alcohol, emotional states and their attitudes towards smoking MA were important factors that contribute to reinitiation and relapse. Surveillance systems, antidrug social workers, vocational skills trainings, moving to another city and family responsibility might serve as counter measures targeti叩 those mentioned weaknesses and threats above. Conclusion This SWOT analysis highlights the complex nature of relapse. Comprehensive interventions strengtheni叩 coping skills such as virtual reality techniques are desperately needed to facilitate individuals1 sustained abstinence. | Youwei Zhu Na Zhong Hang Su Xiaolu Ruan Jiayi Bao Lei Zhang Jiang Du Ding Xu Rongjie Ding Ke Xiao Min Zhao | 2019 | General Psychiatry2019，32，3： | 9 |
| 2 | BubR1 phosphorylates CENP-E as a switch enabling the transition from lateral association to end-on capture of spindle microtubules显示文摘Error-free mitosis depends on accurate chromosome attachment to spindle microtubules, powered congression of those chromosomes, their segregation in anaphase, and assembly of a spindle midzone at mitotic exit. The centromere-associated kinesin motor CENP-E, whose binding partner is BubR1, has been implicated in congression of misaligned chromosomes and the transition from lateral kinetochore-microtubule association to end-on capture. Although previously proposed to be a pseudokinase, here we report the structure of the kinase domain of Drosophila melanogaster BubR1, revealing its folding into a conformation predicted to be catalytically active. BubR1 is shown to be a bona fide kinase whose phosphorylation of CENP-E switches it from a laterally attached microtubule motor to a plus-end microtubule tip tracker. Computational modeling is used to identify bubristatin as a selective BubR1 kinase antagonist that targets the αN1 helix of N-terminal extension and αC helix of the BubR1 kinase domain. Inhibition of CENP-E phosphorylation is shown to prevent proper microtubule capture at kinetochores and, surprisingly, proper assembly of the central spindle at mitotic exit. Thus, BubR1-mediated CENP-E phosphorylation produces a temporal switch that enables transition from lateral to end-on microtubule capture and organization of microtubules into stable midzone arrays. | Yuejia Huang Lin Lin Xing Liu Sheng Ye Phil Y. Yao Wenwen Wang Fengrui Yang Xinjiao Gao Junying Li Yin Zhang Jiancun Zhang Zhihong Yang Xu Liu Zhenye Yang Jianye Zang Maikun Teng Zhiyong Wang Ke Ruan Xia Ding Lin Li Don W. Cleveland Rongguang Zhang Xuebiao Yao | 2019 | Cell Research2019，29，7： | 8 |
| 3 | Mitosis-specific acetylation tunes Ran effector binding for chromosome segregation显示文摘在房间期间,分割要求忠诚有丝分裂的锭子汇编和染色体分离的基因信息的稳定的传播。运用了 GTPase 戏在有丝分裂的锭子汇编的一个关键角色。然而,在锭子的 Ran-GTP 的一个化学坡度的产生怎么被联合到有丝分裂的 translational 以后修正,从来没被描绘过。这里,我们解决了复杂结构与核苷酸版本因素 Mog1 跑了并且描出一个新奇有丝分裂特定的调整 acetylation 的 Ran-Mog1 相互作用在染色体分离期间。我们的指导结构的功能的分析表明那 Mog1 与 RCC1 竞争为以一种 GTP/GDP-dependent 方式变有约束力。生物化学的描述表明了那 Mog1 固定跑阻止 RCC1 有约束力、随后的 GTP 装载。令人惊讶地,跑了是 TIP60 的真正的底层,并且由 TIP60 的 Lys134 的 acetylation 解放 Mog1 从在有丝分裂期间变有约束力。重要地,这个得到 acetylation 的开关对 RCC1 变有约束力支持 Ran-GTP 的高水平,它为染色体排列是必要的。这些结果建立一以前 TIP60 由调节在提供 Ran-GTP 水平的 homeostatic 控制的规章的机制运用了的 uncharacterized 为在有丝分裂的染色体分离的受动器绑定。 | Xiaoling Bao Heng Liu Xing Liu Ke Ruan Yonshui Zhang Zhiyong Zhang Qi Hu Ying Liu Saima Akram Jiahai Zhang Qingguo Gong Wenwen wang Xiao Yuan Jian-Li Lingli Zhao Zhen Dou Ruijun Tian Xuebiao Yao Jihui Wu Yunyu Shi | 2018 | Journal of Molecular Cell Biology2018，10，1： | 8 |
| 4 | Gallic acid inhibits fibroblast growth and migration in keloids through the AKT/ERK signaling pathway显示文摘Keloids are a fibroproliferative disorder of the skin resulted from abnormal healing of injured or irritated skin and are characterized by the ability to spread beyond the original boundary of the wound.Here,we tested the effect of gallic acid (GA),a plant polyphenol with selective growth inhibitory effects in cancer,on the proliferation and invasion of keloid fibroblasts (KFs)isolated from patients undergoing surgery.GA inhibited KF proliferation,migration,and invasion in parallel with the downregulation of matrix metalloproteinase-1 and -3 and upregulation of tissue inhibitors of metalloproteinase-1.Flow cytometric analysis showed that GA inhibited cell cycle progression and induced apoptosis.The effects of GA on KFs occurred in parallel with the inhibition of AKT and ERK1/2,suggesting that GA acts by suppressing the AKT/ERK signaling pathway.In ex vivo explant cultures of keloid tissues,GA inhibited the migration of KFs to the wound area and suppressed the expression of angiogenic markers concomitant with the inhibition of collagen deposition.These results identify GA as a potential therapeutic agent for the treatment of keloids and suggest a potential mechanism underlying its protective effect. | Xiuxia Wang Ke Liu Mengying Ruan Jun Yang Zhen Gao | 2018 | Acta Biochimica et Biophysica Sinica2018，50，11： | 6 |
| 5 | U-Pb Zircon and Re-Os Molybdenite Geochronology of the W-Mo Mineralized Region of South Qinling, China, and their Tectonic Implications显示文摘A W-Mo mineralized region is located along the northern margin of the South Qinling tectonic belt of China. WMo mineralization occurs mainly in Cambrian–Ordovician clastic and carbonate rocks, and the ore bodies are structurally controlled by NW–SE-and NNE–SSW-striking faults. Evidence for magmatism in the area is widespread and is dominated by intermediate–felsic intrusives or apophyses, such as the Dongjiangkou, Yanzhiba, Lanbandeng, and Sihaiping granitic bodies. Quartz-vein-type mineralization and fault-controlled skarn-type mineralization dominate the ore systems, with additional enrichment in residual deposits. At present, there are few or insufficient studies on(1) the age of mineralization,(2) the relationship between intermediate–felsic granite and W-Mo mineralization,(3) the source of ore-forming materials, and(4) the metallogenic and tectonic setting of the mineralized area. In this paper, we present geochronology results for numerous intrusive granitic bodies in the South Qinling tectonic belt. U-Pb zircon geochronology of the Lanbandeng monzogranite and Wangjiaping biotite monzogranite yields ages of 222.7 ± 2.3 and 201.9 ± 1.8 Ma, respectively. In contrast to the Late Triassic age of the Lanbandeng monzogranite, the age of the newly discovered Wangjiaping biotite monzogranite places it at the Triassic–Jurassic boundary. Re-Os molybdenite geochronology on the Qipangou W-Mo deposit yielded a model age of 199.7 ± 3.9 Ma, indicating the deposit formed in the early Yanshanian period of the Early Jurassic. Granitoid intrusions in the mineralized area are characterized by composite granite bodies that crystallized at ca. 240–190 Ma. While there were multiple stages of intrusion, most occurred at 210–220 Ma, with waning magmatic activity at 200–190 Ma. The Re-Os age of molybdenite in the region is ca. 200–190 Ma, which may represent a newly discovered period of W-Mo metallogenesis that occurred during the final stages of magmatism. The heat associated with this magmatism drove ore formation and might have provided additional ore-forming components for metallogenesis(represented by the Wangjiaping biotite monzogranite). Ore materials in the mineralized area were derived from mixed crustal and mantle sources. Enrichment of the region occurred during intracontinental orogenesis in the late Indosinian–Yanshanian, subsequent to the main Indosinian collision. At this time, the tectonic environment was dominated by extension and strike-slip motion. | HAN Ke YANG Xingke CHAO Huixia HE Hujun RUAN Shiqi GAO Yunfeng ZHANG Weisheng ZHU Wei JIN Gang | 2021 | Acta Geologica Sinica(English Edition)2021，95，2： | 3 |
| 6 | Mitotic motor CENP-E cooperates with PRC1 in temporal control of central spindle assembly显示文摘Error-free cell division depends on the accurate assembly of the spindle midzone from dynamic spindle microtubules to ensure chromatid segregation during metaphase-anaphase transition.However,the mechanism underlying the key transition from the mitotic spindle to central spindle before anaphase onset remains elusive.Given the prevalence of chromosome instability phenotype in gastric tumorigenesis,we developed a strategy to model context-dependent cell division using a combination of light sheet microscope and 3D gastric organoids.Light sheet microscopic image analyses of 3D organoids showed that CENP-E inhibited cells undergoing aberrant metaphase-anaphase transition and exhibiting chromosome segregation errors during mitosis.Highresolution real-time imaging analyses of 2D cell culture revealed that CENP-E inhibited cells undergoing central spindle splitting and chromosome instability phenotype.Using biotinylated syntelin as an affinity matrix,we found that CENP-E forms a complex with PRC1 in mitotic cells.Chemical inhibition of CENP-E in metaphase by syntelin prevented accurate central spindle assembly by perturbing temporal assembly of PRC1 to the midzone.Thus,CENP-E-mediated PRC1 assembly to the central spindle constitutes a temporal switch to organize dynamic kinetochore microtubules into stable midzone arrays.These findings reveal a previously uncharacterized role of CENP-E in temporal control of central spindle assembly.Since CENP-E is absent from yeast,we reasoned that metazoans evolved an elaborate central spindle organization machinery to ensure accurate sister chromatid segregation during anaphase and cytokinesis. | Xu Liu Leilei Xu Junying Li Phil Y.Yao Wanjuan Wang Hazrat Ismail Haowei Wang Bryce Liao Zhihong Yang Tarsha Ward Ke Ruan Jianchun Zhang Quan Wu Ping He Xia Ding Dongmei Wang Chuanhai Fu Zhen Dou Feng Yan Wenwen Wang Xing Liu Xuebiao Yao | 2020 | Journal of Molecular Cell Biology2020，12，8： | 3 |
| 7 | Studies of Multi-Allelic Polymorphism of Dominant Dwarfing Genes in Wheat显示文摘Dwarfing breeding of wheat in the world is confined to the exploitation of recessive dwarfing sources. None of the dominant dwarfing sources discovered in common wheat (Triticum aestivum L.) has found wide exploitation in wheat breeding due to the extreme dwarfness of their plants (2055 cm). We found in our work that some stable mutant lines with their plant height enhanced to different extents could be obtained in large populations derived from the stock seeds of the dominant dwarfing sources Aibian1 carrying Rht10 on 4DS and being 2055 cm tall and Aisu2 carrying Rht3 on 4BS and being 55 cm tall, or from their descendants of induced mutation treatments, or from the segregating descendants of their crosses with mid- or tall-statured genotypes. Subsequently, we studied these mutation-derived lines differing in plant height with near isogenic lines and observed that the character of their enhanced plant height bred true, each carrying a semi-dominant dwarfing gene for a definite height and that as the plant height of the mutation-derived lines increased, the yield-contributing characters of their near isogenic lines were significantly improved. When test crosses with marker genes and physiological and biochemical genetic marker tests were performed to re-localize the semi-dominant dwarfing genes carried by the mutation-derived lines, it was confirmed that they shared common loci with Rht10 and Rht3 and that they were all mutation-derived multiple alleles. It is thus speculated that dominant dwarfing genes are of 'multi-allelic polymorphism'. In other words, dominant dwarfing genes, which are ultra-dwarfing, are liable to develop by mutation into a group of multiple alleles with plant height enhanced to different extents and some may have a height close to the ideal plant height for wheat breeding. Therefore, these results offer a fundamentally new approach for the exploitation of dominant dwarfing sources in wheat breeding. | FU Da-xiong, RUAN Ren-wu, YIN Jia-min, ZONG Xue-feng, DAI Xiu-mei, ZHANG Jian-kui, LI Ke, QU Jie and WANG Yi-min (College of Agronomy and Life Science, Southwest Agricultural University, Chongqing 400716, P.R.China) | 2003 | Agricultural Sciences in China2003，2，12： | 2 |
| 8 | Two new ent-kaurane diterpenoids from the aerial parts of Isodon excisoides显示文摘Two new ent-kaurane diterpenoids, ent-7a,14b-dihydroxykaur-16-en-15-one-20-oic acid(1) and 1,7a,12b,14b-tetrahydroxy-1,10-seco-ent-kaur-10,16-dien-15-one(2), together with six known ones(3–8), were isolated from the EtOAc extract of the aerial parts of Isodon excisoides. Their structures were elucidated on the basis of 1D NMR and 2D NMR analyses as well as HR-ESI-MS experiments. | Ke Jiao Huo-Yun Li Peng Zhang Hui-Fang Pi Han-Li Ruan Ji-Zhou Wu | 2014 | Chinese Chemical Letters2014，25，1： | 2 |
| 9 | Prediction of three di-mensional structure of calmodulin显示文摘 | Chen Ke Ruan Jishou Kurgan L A | 2006 | Protein J2006，25，1： | 1 |
| 10 | Preparation and performance of porous phase change polyethylene glycol/polyurethane membrane 显示文摘 | KE Guizhen XIE Huifang RUAN Ruping | 2010 | Ener- gy Conversion and Management2010，51，11： | 1 |
| 11 | Structures of human bile acid exporter ABCB11 reveal a transport mechanism facilitated by two tandem substrate-binding pockets显示文摘Dear Editor,Bile acids are a group of cholesterol-derived physiological surfactants required for digestion of lipids and fat-soluble vitamins.1However,excess of bile acids is detrimental to metabolic homeostasis and bile acid imbalance can contribute to cancer progression.2Bile acids are produced primarily in the liver and then secreted to the bile duct before delivery to the small intestine,where up to 95%of bile acids are reabsorbed and recirculated to the liver(Supplementary information,Fig.S1a).The primary bile acids are biosynthesized in hepatocytes via oxidation of cholesterol and modified by either taurine or glycine,forming the conjugated primary bile acids which harbor a cholic acid moiety and a taurine/glycine moiety(Supplementary information,Fig.S1 b).This enterohepatic circulation of bile acids is driven by a series of transmembrane transporters(Supplementary informa-tion,Fig.S1a),including the ATP-binding cassette(ABC)transpor-ter ABCB11(also termed bile salt export pump BSEP). | Liang Wang Wen-Tao Hou Jie Wang Da Xu Cong Guo Linfeng Sun Ke Ruan Cong-Zhao Zhou Yuxing Chen | 2022 | Cell Research2022，32，5： | 1 |
| 12 | Bcl-2 and P53 oncoproteins expression during colorectal tumorigenesis 显示文摘 | Sinicrope FA Ruan SB Cleary KE | 1995 | Cancer Res1995，55，2： | 1 |
| 13 | Three-level bidirectional converter for fuel-cell/battery hybrid power system显示文摘 | Jin Ke Yang Mengxiong Ruan Xinbo | 2010 | IEEE Trans on Industry Applications2010，57，6： | 1 |
| 14 | Research on rapid repairing techniques for auto panel dies显示文摘 | J.K. Ruan Y.L. Ke S.Q. Fan H.Y. Dong | 2006 | Journal of Materials Processing Tech2006，，： | 1 |
| 15 | Engineering subtilisin into a fluoride-triggered processing protease useful for one-step protein purification显示文摘 | Ruan B Fisher KE Alexander PA | 2004 | Biochemistry2004，43，14： | 1 |
| 16 | Autophagy inhibition enhances isorhamnetin?induced mitochondria?dependentapoptosis in non?small cell lung cancer cells显示文摘 | Yushu Ruan Ke Hu Hongbo Chen | 2015 | Molecular Medicine Reports2015，，4： | 1 |
| 17 | Research on rapid repair- ing techniques for auto panel dies显示文摘 | Ruan J K Ke Y L Fan S Q | 2007 | Journal of Materials Processing Technology2007，18，： | 1 |
| 18 | Hybrid Full-Bridge Three-Level LLC Resonant Converter-A Novel DC DC Converter Suitable for Fuel-Cell Power System显示文摘 | Ke Jin and Xinbo Ruan | 2006 | IEEE Transactions on Industrial Electronics2006，53，5： | 1 |
| 19 | Jahn-Teller distortion assisted interstitial nitrogen engineering:Enhanced oxygen dehydrogenation activity of N-doped Mn_(x)Co_(3-x)O_(4) hierarchical micro-nano particles显示文摘Rational design of earth-abundant transition metal oxides catalysts is highly desirable for developing sustainable chemical processes.Herein,we demonstrate a prospective interstitial nitrogen engineering for fabricating oxygen vacancies(OVs)-rich nitrogen-doped-Mn_(x)Co_(3-x)O_(4)(N-Mn_(x)Co_(3-x)O_(4))oxide catalyst,in which the ratio of OVs concentration of N-Mn_(x)Co_(3-x)O_(4)to Mn species is as high as 1:1,according to the characterizations of X-ray absorption(XAS)and X-ray photoelectron(XPS)spectroscopies.The promising strategy of interstitial nitrogen engineering through lattice distortion caused by the Jahn-Teller effect can significantly increase the amount of interstitial nitrogen.The resulting catalyst enables an additive-free aerobic dehydrogenation coupling of aromatic amine to afford azo compounds with>99%yield and>99%selectivity at 60☆.We observed the superb catalytic activity is promoted by the enhanced oxygen mobility in OVs,which were created by the interstitial nitrogen in the catalyst matrix.The presence of interstitial nitrogen in transition metal oxides in this study shows how the manipulation of catalyst matrix can increase the OV sites to promote aerobic oxidation reaction. | Yangxin Jin Fengfeng Li Peixin Cui Yun Yang Qingping Ke Minh Ngoc Ha Wangcheng Zhan Fei Ruan Chao Wan Zhao Lei Van Noi Nguyen Wei Chen Jun Tang | 2021 | Nano Research2021，14，8： | 1 |
| 20 | Patients with Prolonged Positivity of SARS-CoV-2 RNA Benefit from Convalescent Plasma Therapy:A Retrospective Study显示文摘Convalescent plasma therapy has been implemented in a few cases of severe coronavirus disease 2019.No report about convalescent plasma therapy in treating patients with prolonged positivity of SARS-CoV-2 RNA has been published.In this study,we conducted a retrospective observational study in 27 patients with prolonged positivity of SARS-CoV-2 RNA,the clinical benefit of convalescent plasma therapy were analyzed.q RT-PCR test of SARS-CoV-2 RNA turned negative(B 7 days)in a part of patients(early negative group,n=15)after therapy,others(late negative group,n=12)turned negative in more than 7 days.Pulmonary imaging improvement was confirmed in 7 patients in early negative group and 8 in late negative group after CP therapy.Viral load decreased in early negative group compared with late negative group at day 3,5,7 after implementing convalescent plasma therapy.Patients in early negative group had a shorter median length of hospital stay.In conclusion,convalescent plasma therapy might help eliminate virus and shorten length of hospital stay in patients with prolonged positivity of SARS-CoV-2 RNA. | Yongran Wu Ke Hong Lianguo Ruan Xiaobo Yang Jiancheng Zhang Jiqian Xu Shangwen Pan Lehao Ren Lu Chen Chaolin Huang You Shang | 2020 | Virologica Sinica2020，35，6： | 1 |
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