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| 1 | Relative Contributions of Boundary-Layer Meteorological Factors to the Explosive Growth of PM2.5 during the Red-Alert Heavy Pollution Episodes in Beijing in December 2016显示文摘Based on observations of urban mass concentration of fine particulate matter smaller than 2.5 μm in diameter(PM_(2.5)), ground meteorological data, vertical measurements of winds, temperature, and relative humidity(RH), and ECMWF reanalysis data, the major changes in the vertical structures of meteorological factors in the boundary layer(BL) during the heavy aerosol pollution episodes(HPEs) that occurred in winter 2016 in the urban Beijing area were analyzed. The HPEs are divided into two stages: the transport of pollutants under prevailing southerly winds, known as the transport stage(TS), and the PM_(2.5) explosive growth and pollution accumulation period characterized by a temperature inversion with low winds and high RH in the lower BL, known as the cumulative stage(CS). During the TS, a surface high lies south of Beijing, and pollutants are transported northwards. During the CS, a stable BL forms and is characterized by weak winds, temperature inversion, and moisture accumulation. Stable atmospheric stratification featured with light/calm winds and accumulated moisture(RH > 80%) below 250 m at the beginning of the CS is closely associated with the inversion, which is strengthened by the considerable decrease in near-surface air temperature due to the interaction between aerosols and radiation after the aerosol pollution occurs. A significant increase in the PLAM(Parameter Linking Aerosol Pollution and Meteorological Elements) index is found, which is linearly related to PM mass change. During the first 10 h of the CS, the more stable BL contributes approximately 84% of the explosive growth of PM_(2.5) mass. Additional accumulated near-surface moisture caused by the ground temperature decrease, weak turbulent diffusion, low BL height, and inhibited vertical mixing of water vapor is conducive to the secondary aerosol formation through chemical reactions, including liquid phase and heterogeneous reactions, which further increases the PM_(2.5) concentration levels. The contribution of these reaction mechanisms to the explosive growth of PM_(2.5) mass during the early CS and subsequent pollution accumulation requires further investigation. | junting zhong xiaoye zhang yaqiang wang junying sun yangmei zhang jizhi wang kaiyan tan xiaojing shen haochi che lu zhang zhouxiang zhang xuefei qi huarong zhao sanxue ren yang li | 2017 | Journal of Meteorological Research2017，31，5： | 25 |
| 2 | Minimal invasive microscopic tooth preparation in esthetic restoration: a specialist consensus显示文摘By removing a part of the structure,the tooth preparation provides restorative space,bonding surface,and finish line for various restorations on abutment.Preparation technique plays critical role in achieving the optimal result of tooth preparation.With successful application of microscope in endodontics for>30 years,there is a full expectation of microscopic dentistry.However,as relatively little progress has been made in the application of microscopic dentistry in prosthodontics,the following assumptions have been proposed:Is it suitable to choose the tooth preparation technique under the naked eye in the microscopic vision?Is there a more accurate preparation technology intended for the microscope?To obtain long-term stable therapeutic effects,is it much easier to achieve maximum tooth preservation and retinal protection and maintain periodontal tissue and oral function health under microscopic vision?Whether the microscopic prosthodontics is a gimmick or a breakthrough in obtaining an ideal tooth preparation should be resolved in microscopic tooth preparation.This article attempts to illustrate the concept,core elements,and indications of microscopic minimally invasive tooth preparation,physiological basis of dental pulp,periodontium and functions involved in tool preparation,position ergonomics and visual basis for dentists,comparison of tooth preparation by naked eyes and a microscope,and comparison of different designs of microscopic minimally invasive tooth preparation techniques.Furthermore,a clinical protocol for microscopic minimally invasive tooth preparation based on target restorative space guide plate has been put forward and new insights on the quantity and shape of microscopic minimally invasive tooth preparation has been provided. | Haiyang Yu Yuwei Zhao Junying Li Tian Luo Jing Gao Hongchen Liu Weicai Liu Feng Liu Ke Zhao Fei Liu Chufan Ma Juergen MSetz Shanshan Liang Lin Fan Shanshan Gao Zhuoli Zhu Jiefei Shen Jian Wang Zhimin Zhu Xuedong Zhou | 2019 | International Journal of Oral Science2019，11，4： | 25 |
| 3 | Sedimentary Characteristics and Paleoenvironmental Records of Zabuye Salt Lake,Tibetan Plateau,since 128 ka BP显示文摘This paper for the first time reveals high-resolution core records of Zabuye Salt Lake in the interior of the Qinghai-Tibet Plateau.According to 1346 samples taken continuously,relatively accurate ^(14)C,U-series disequilibrium and ESR ages have been obtained,thus revealing that the lake core ages from 0 to 83.63m of hole SZK02 are~800 to over 128 ka.In the paper,the lake core sedimentary characteristics(including the lithologies and mineral assemblages)are analyzed in detail and correlated with ostracod assemblages I to XX and sporopollen zones A to I,and on the basis of an integrated analysis of theδ^(18)0 values of authigenic calcium-magnesium carbonate and environmental proxies of minerals,sporopollen and microfossils in the lake core,a correlation has been made of oxygen isotope change between this lake core and the Greenland GISP2 and GRIP and Guliya ice cores,and the climate of Zabuye Salt Lake since 128 ka BP is divided into the last interglacial stage (including substages e,d,c,b and a)of oxygen isotope stage(OIS)5,early glacial stadial of the last glacial stage of OIS 4,interglacial stadial of the last glacial stage of OIS 3,late glacial stadial of the last glacial stage or Last Glacial Maximum of OIS 2 and postglacial state of OIS 1;in addition,6 Heinrich (H_6-H_1)events,Younger Dryas event and 8.2 ka BP cold event have been recognized. | ZHENG Mianping YUAN Heran LIU Junying LI Yanhe MA Zhibang SUN Qing | 2007 | Acta Geologica Sinica(English Edition)2007，81，5： | 10 |
| 4 | Distinct element method analysis and field experiment of soil resistance applied on the subsoiler显示文摘Since the design of the subsoiler is a complex work,the interaction between the subsoiler and soil was investigated by using Distinct Element Method(DEM)in this study.Based on the traditional discrete element theory,the 3D model of soil particles and the subsoiler were established after considering the liquid bridge force between soil particles.The operating resistance curves of the subsoiler were achieved after the DEM simulation at a speed of 1 m/s,and three depths of 180 mm,220 mm and 260 mm,respectively.The simulation curves agreed well with the field experimental results based on relative errors of 2.96%,14.95%and 7.15%,respectively,at three depths.All these data proved that it was feasible and favorable to analyze the performance of the subsoiler by using the DEM and it is of important significance for studying and further optimizing the structure of the subsoiler. | Li Bo Liu Fanyi Mu Junying Chen Jun Han Wenting | 2014 | International Journal of Agricultural and Biological Engineering2014，7，1： | 9 |
| 5 | Preparation and properties of tungsten-doped indium oxide thin films显示文摘Tungsten-doped indium oxide (IWO) thin films were deposited on glass substrate by DC reactive magnetron sputtering. The effects of sputtering power and growth temperature on the structure, surface morphology, optical and electrical properties of IWO thin films were investigated. The thickness and surface morphology of the films are both closely dependent on the sputtering power and the substrate temperature. The transparency of the films decreases with the increase of the sputtering power but is not seriously influenced by substrate temperature. All the IWO thin film samples have high transmittance in near-infrared spectral range. With either the sputtering power or the growth temperature increases, the resistivity of the film decreases at the beginning and increases after the optimum parameters. The as-deposited IWO films with minimum resistivity of 6.4 10 4 cm were obtained at a growth temperature of225 C and sputteringpower of 40 W, with carrier mobility of 33.0 cm 2 V 1 s 1 and carrier concentration of 2.8 10 20 cm 3 and the average transmittance of about 81% in near-infrared region and about 87% in visible region. | Li, Yuan Wang, Wenwen Zhang, Junying Wang, Rongming | 2012 | Rare Metals2012，31，2： | 9 |
| 6 | Prediction of Cross-Tension Strength of Self-Piercing Riveted Joints Using Finite Element Simulation and XGBoost Algorithm显示文摘Self-piercing riveting(SPR)has been widely used in automobile industry,and the strength prediction of SPR joints always attracts the attention of researchers.In this work,a prediction method of the cross-tension strength of SPR joints was proposed on the basis of finite element(FE)simulation and extreme gradient boosting decision tree(XGBoost)algorithm.An FE model of SPR process was established to simulate the plastic deformations of rivet and substrate materials and verified in terms of cross-sectional dimensions of SPR joints.The residual mechanical field from SPR process simulation was imported into a 2D FE model for the cross-tension testing simulation of SPR joints,and cross-tension strengths from FE simulation show a good consistence with the experiment result.Based on the verified FE model,the mechanical properties and thickness of substrate materials were varied and then used for FE simulation to obtain cross-tension strengths of a number of SPR joints,which were used to train the regression model based on the XGBoost algorithm in order to achieve prediction for cross-tension strength of SPR joints.Results show that the cross-tension strengths of SPR steel/aluminum joints could be successfully predicted by the XGBoost regression model with a respective error less than 7.6%compared to experimental values. | Jianping Lin Chengwei Qi Hailang Wan Junying Min Jiajie Chen Kai Zhang Li Zhang | 2021 | Chinese Journal of Mechanical Engineering2021，34，2： | 8 |
| 7 | BubR1 phosphorylates CENP-E as a switch enabling the transition from lateral association to end-on capture of spindle microtubules显示文摘Error-free mitosis depends on accurate chromosome attachment to spindle microtubules, powered congression of those chromosomes, their segregation in anaphase, and assembly of a spindle midzone at mitotic exit. The centromere-associated kinesin motor CENP-E, whose binding partner is BubR1, has been implicated in congression of misaligned chromosomes and the transition from lateral kinetochore-microtubule association to end-on capture. Although previously proposed to be a pseudokinase, here we report the structure of the kinase domain of Drosophila melanogaster BubR1, revealing its folding into a conformation predicted to be catalytically active. BubR1 is shown to be a bona fide kinase whose phosphorylation of CENP-E switches it from a laterally attached microtubule motor to a plus-end microtubule tip tracker. Computational modeling is used to identify bubristatin as a selective BubR1 kinase antagonist that targets the αN1 helix of N-terminal extension and αC helix of the BubR1 kinase domain. Inhibition of CENP-E phosphorylation is shown to prevent proper microtubule capture at kinetochores and, surprisingly, proper assembly of the central spindle at mitotic exit. Thus, BubR1-mediated CENP-E phosphorylation produces a temporal switch that enables transition from lateral to end-on microtubule capture and organization of microtubules into stable midzone arrays. | Yuejia Huang Lin Lin Xing Liu Sheng Ye Phil Y. Yao Wenwen Wang Fengrui Yang Xinjiao Gao Junying Li Yin Zhang Jiancun Zhang Zhihong Yang Xu Liu Zhenye Yang Jianye Zang Maikun Teng Zhiyong Wang Ke Ruan Xia Ding Lin Li Don W. Cleveland Rongguang Zhang Xuebiao Yao | 2019 | Cell Research2019，29，7： | 8 |
| 8 | Acoustic reflection of rib-stiffened double plates coated with a viscoelastic layer显示文摘To predict sound-absorbing performance of anechoic materials,the acoustic reflection problem of a viscoelastic layer backed with periodically rib-stiffened infinite double plates is studied in this paper.The reason why structural theories of plates are not applicable to viscoelastic plates is explained through comparing dispersion and attenuation curves of flexural waves with those of Lamb waves.As a result,(visco-)elastic theory is adopted to deal with(visco-)elastic plates,and ribs are treated by structural theories of plates.The coupling between ribs and plates are solved by Hull's method,and solution of the reflected field is obtained.The accuracy of present method is validated by comparing with the results by the structural theories of plates.The influence of a backing on the acoustic reflection of the viscoelatic layer is analyzed by computing reflection coefficients.Performances of different viscoelastic materials are evaluated by the average reflection coefficients.The computational results show that,influence of a backing on the acoustic reflection cannot be suppressed by the viscoelastic materials in low frequencies.The resonance is determined by the coupling of the fluid layer and the double plates.And ribs,which are coupled with the double plates,mainly reduce the acoustic reflection. | GONG Jiayuan AN Junying MA Li XU Haiting | 2014 | Chinese Journal of Acoustics2014，33，3： | 6 |
| 9 | The mid-high latitude whistler mode chorus waves observed around substorm onsets显示文摘Using the data of LFEW/TC-2, we studied the dawn side chorus around substorm onsets during a strong geomagnetic storm in November 2004. During this storm, LFEW/TC-2 observed 14 dawnside chorus events. Nine of them were associated with substorms and occurred within 40 min around the substorm onsets. The fre-quencies of waves have a very good correlation with the half equatorial electron cyclotron frequencies. Chorus can be excited in the region near magnetic equato-rial plane and then propagate to the mid and high latitudes. When the wave fre-quencies reach the local lower hybrid frequencies, chorus can be reflected due to the lower hybrid resonance. The time delay between the chorus and its echo is about 28 min. Previous observations show that the chorus can propagate at most to the magnetic latitudes of 40°. LFEW/ TC-2 found for the first time that the chorus in space could propagate to the magnetic latitude of 70°. Since most of the previous chorus observations are made close to the magnetic equatorial plane, our results are important for the studies of excitation and propagation of whistler mode wave, and relevant relativistic electron acceleration in the magnetosphere. | YANG JunYing CAO JinBin YAN ChunXiao LI LiuYuan MA YuDuan | 2008 | Science China(Technological Sciences)2008，51，10： | 6 |
| 10 | SARS-CoV-2 promotes RIPK1 activation to facilitate viral propagation显示文摘Coronavirus disease 2019(COVID-19),caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),is the ongoing global pandemic that poses substantial challenges to public health worldwide.A subset of COVID-19 patients experience systemic inflammatory response,known as cytokine storm,which may lead to death.Receptor-interacting serine/threonine-protein kinase 1(RIPK1)is an important mediator of inflammation and cell death.Here,we examined the interaction of RIPK1-mediated innate immunity with SARS-CoV-2 infection.We found evidence of RIPK1 activation in human COVID-19 lung pathological samples,and cultured human lung organoids and ACE2 transgenic mice infected by SARS-CoV-2.Inhibition of RIPK1 using multiple smallmolecule inhibitors reduced the viral load of SARS-CoV-2 in human lung organoids.Furthermore,therapeutic dosing of the RIPK1 inhibitor Nec-1s reduced mortality and lung viral load,and blocked the CNS manifestation of SARS-CoV-2 in ACE2 transgenic mice.Mechanistically,we found that the RNA-dependent RNA polymerase of SARS-CoV-2,NSP12,a highly conserved central component of coronaviral replication and transcription machinery,promoted the activation of RIPK1.Furthermore,NSP12323L variant,encoded by the SARS-CoV-2 C14408T variant first detected in Lombardy,Italy,that carries a Pro323Leu amino acid substitution in NSP12,showed increased ability to activate RIPK1.Inhibition of RIPK1 downregulated the transcriptional induction of proinflammatory cytokines and host factors including ACE2 and EGFR that promote viral entry into cells.Our results suggest that SARS-CoV-2 may have an unexpected and unusual ability to hijack the RIPK1-mediated host defense response to promote its own propagation and that inhibition of RIPK1 may provide a therapeutic option for the treatment of COVID-19. | Gang Xu Ying Li Shengyuan Zhang Haoran Peng Yunyun Wang Dekang Li Taijie Jin Zhuohao He Yilun Tong Chunting Qi Guowei Wu Kangyun Dong Jizhou Gou Yang Liu Tongyang Xiao Jing Qu Liang Li Liang Liu Ping Zhao Zheng Zhang Junying Yuan | 2021 | Cell Research2021，31，12： | 6 |
| 11 | Hierarchical-structure anatase TiO2 with conductive network for high-rate and high-loading lithium-ion battery显示文摘Titanium dioxides have been extensively investigated as promising anodes for Lithium ion batteries(LIBs)because of the high–rate capacity and cyclability,as well as the improved safety over graphite anode(1,2)However,as a typical insertion–type anode,anatase TiO2 exhibits low conductivity(10–12S cm-1 for electron conductivity[3]and 10–17–10–10 cm2 s1 for Li+ion diffusion coefficient[4])and poor specific capacity(only accommodate<0.5 Li per bulk TiO2 unit[5]),severely limiting its practical applications. | Xinghua Liu Lijiang Zhao Shitong Wang Meng Chao Yutong Li Jin Leng Junying Zhang Zilong Tang | 2019 | Science Bulletin2019，64，16： | 6 |
| 12 | LRIF1 interacts with HPla to coordinate accurate chromosome segregation during mitosis显示文摘Heterochromatin protein 1α (HP1α)regulates chromatin specification and plasticity during cell fate decision.Different structural determinants account for HP1α Localization and function during cell division cycle.Our earlier study showed that centromeric Localization of HP1α depends on the epigenetic mark H3K9me3 in interphase,while its centromeric location in mitosis relies on uncharacterized PXVXL-containing factors.Here,we identified a PXVXL-containing protein,Ligand-dependent nuclear receptorinteracting factor 1 (LRIF1),which recruits HPla to the centromere of mitotic chromosomes and its interaction with HP1α is essential for accurate chromosome segregation during mitosis.LRIF1 interacts directly with HPla chromoshadow domain via an evolutionariLy conserved PXVXL motif within its C-terminus.Importantly,the LRIF1-HPla interaction is critical for Aurora B activity in the inner centromere.Mutation of PXVXL motif of LRIF1 Leads to defects in HPla centromere targeting and aberrant chromosome segregation.These findings reveal a previously unrecognized direct Link between LRIF1 and HP1α in centromere plasticity control and illustrate the critical role of LRIF1-HP1α interaction in orchestrating accurate cell division. | Saima Akram Fengrui Yang Junying Li Gregory Adams Yingying Liu Xiaoxuan Zhuang Lingluo Chu Xu Liu Nerimah Emmett Winston Thompson McKay Mullen Saravana Muthusamy Wenwen Wang Fei Mo Xing Liu | 2018 | Journal of Molecular Cell Biology2018，10，6： | 5 |
| 13 | Exergy life cycle assessment model of “CO_2 zero-emission” energy system and application显示文摘An exergy life cycle assessment(ELCA) model based on life cycle assessment(LCA) and exergy methodology was developed to assess a 2×300 MW coal-fired power plant,and the results indicated that the exergy input in operation phase of power plant accounts for 99.89% of the total input and only 0.11% in construction and decommission phases. Direct and indirect exergy inputs account for 93.03% and 6.97%,respectively. Compared with coal-fired power generation system before carbon emission reduction,exergy input-output ratio of life cycle 'CO2 zero-emission' energy system and exergy efficiency are about 5.563 and 17.97%,respectively,which increases by 62.47% and declines by 11.21% approximately. The model quantifies the energy,resource consumption and pollutant emissions of system life cycle using exergy as the basic physical parameter,which will make the assessment more objective and reasonable. | WANG Yun ZHANG JunYing ZHAO YongChun LI ZhongYuan ZHENG ChuGuang | 2011 | Science China(Technological Sciences)2011，54，12： | 5 |
| 14 | Highly efficient catalytic scavenging of oxygen free radicals with graphene-encapsulated metal nanoshields显示文摘 | Junying Wang Xiaoju Cui Haobo Li Jianping Xiao Jiang Yang Xiaoyu Mu Haixia Liu Yuan-Ming Sun Xuhui Xue Changlong Liu Xiao-Dong Zhang Dehui Deng Xinhe Bao | 2018 | Nano Research2018，11，5： | 4 |
| 15 | The m6A methylation regulates gonadal sex differentiation in chicken embryo显示文摘Background:As a ubiquitous reversible epigenetic RNA modification,N6-methyladenosine(m6A)plays crucial regulatory roles in multiple biological pathways.However,its functional mechanisms in sex determination and differentiation during gonadal development of chicken embryos are not clear.Therefore,we established a transcriptome-wide m6A map in the female and male chicken left gonads of embryonic day 7(E7)by methylated RNA immunoprecipitation sequencing(MeRIP-seq)to offer insight into the landscape of m6A methylation and investigate the post-transcriptional modification underlying gonadal differentiation.Results:The chicken embryonic gonadal transcriptome was extensively methylated.We found 15,191 and 16,111 m6A peaks in the female and male left gonads,respectively,which were mainly enriched in the coding sequence(CDS)and stop codon.Among these m6A peaks,we identified that 1013 and 751 were hypermethylated in females and males,respectively.These differential peaks covered 281 and 327 genes,such as BMP2,SMAD2,SOX9 and CYP19A1,which were primarily associated with development,morphogenesis and sex differentiation by functional enrichment.Further analysis revealed that the m6A methylation level was positively correlated with gene expression abundance.Furthermore,we found that YTHDC2 could regulate the expression of sex-related genes,especially HEMGN and SOX9,in male mesonephros/gonad mingle cells,which was verified by in vitro experiments,suggesting a regulatory role of m6A methylation in chicken gonad differentiation.Conclusions:This work provided a comprehensive m6A methylation profile of chicken embryonic gonads and revealed YTHDC2 as a key regulator responsible for sex differentiation.Our results contribute to a better understanding of epigenetic factors involved in chicken sex determination and differentiation and to promoting the future development of sex manipulation in poultry industry. | Jianbo Li Xiuan Zhang Xiqiong Wang Congjiao Sun Jiangxia Zheng Junying Li Guoqiang Yi Ning Yang | 2022 | Journal of Animal Science and Biotechnology2022，13，6： | 4 |
| 16 | In-flight observations of electromagnetic interferences emitted by satellite显示文摘Using the data from STAFF/TC-1, this paper for the first time analyzes the electromagnetic interferences of Chinese scientific satellite. The electromagnetic interference of satellite exists mainly below 30 Hz, but can extend to 190 Hz with an obviously decreasing power spectral density. The electromagnetic interferences at frequencies below 190 Hz have good correlation with the solar aspect angle. The electromagnetic interferences at frequencies between 190 and 830 Hz have also correlation with solar as-pect angle. However, the electromagnetic interferences at frequencies above 830 Hz have no correlation with the solar aspect angle. The correlation coefficient between solar aspect angel and electromagnetic interferences is around 0.90. The larger the solar aspect angle, the stronger the satellite electromagnetic interference. When the solar aspect angle increases from 90.6° to 93.6°, the electromagnetic interferences at frequencies <10 Hz increase by 8 times and those at frequencies 190―830 Hz increase by 60%. This close association of electromagnetic interferences with the solar aspect angle indicates that the solar aspect angle is the main factor to determine the electromagnetic interferences. The electromagnetic interferences of satellite in sunlight are larger than those in eclipse. The electro-magnetic interference produced by solar panel occupies about 87% in the low frequency band (<100 Hz) and 94% in the high frequency band (>100 Hz) of the total electromagnetic interference produced by satellite. These in flight observations of electromagnetic radiation of satellites will be very helpful to the designs of future satellites of space sciences or earthquake sciences. | CAO JinBin YANG JunYing YUAN ShiGan SHEN XuHui LIU YuanMo YAN ChunXiao LI WenZhen CHEN Tao | 2009 | Science China(Technological Sciences)2009，52，7： | 4 |
| 17 | Numerical quadrature for singular and near-singular integrals of boundary element method and its applications in large-scale acoustic problems显示文摘The numerical quadrature methods for dealing with the problems of singular and near-singular integrals caused by Burton-Miller method are proposed,by which the conventional and fast multipole BEMs(boundary element methods) for 3D acoustic problems based on constant elements are improved.To solve the problem of singular integrals,a Hadamard finite-part integral method is presented,which is a simplified combination of the methods proposed by Kirkup and Wolf.The problem of near-singular integrals is overcome by the simple method of polar transformation and the more complex method of PART(Projection and Angular & Radial Transformation).The effectiveness of these methods for solving the singular and near-singular problems is validated through comparing with the results computed by the analytical method and/or the commercial software LMS Virtual.Lab.In addition,the influence of the near-singular integral problem on the computational precisions is analyzed by computing the errors relative to the exact solution.The computational complexities of the conventional and fast multipole BEM are analyzed and compared through numerical computations.A large-scale acoustic scattering problem,whose degree of freedoms is about 340,000,is implemented successfully.The results show that,the near singularity is primarily introduced by the hyper-singular kernel,and has great influences on the precision of the solution.The precision of fast multipole BEM is the same as conventional BEM,but the computational complexities are much lower. | GONG Jiayuan AN Junying MA Li XU Haiting | 2017 | Chinese Journal of Acoustics2017，36，3： | 4 |
| 18 | Mitotic motor CENP-E cooperates with PRC1 in temporal control of central spindle assembly显示文摘Error-free cell division depends on the accurate assembly of the spindle midzone from dynamic spindle microtubules to ensure chromatid segregation during metaphase-anaphase transition.However,the mechanism underlying the key transition from the mitotic spindle to central spindle before anaphase onset remains elusive.Given the prevalence of chromosome instability phenotype in gastric tumorigenesis,we developed a strategy to model context-dependent cell division using a combination of light sheet microscope and 3D gastric organoids.Light sheet microscopic image analyses of 3D organoids showed that CENP-E inhibited cells undergoing aberrant metaphase-anaphase transition and exhibiting chromosome segregation errors during mitosis.Highresolution real-time imaging analyses of 2D cell culture revealed that CENP-E inhibited cells undergoing central spindle splitting and chromosome instability phenotype.Using biotinylated syntelin as an affinity matrix,we found that CENP-E forms a complex with PRC1 in mitotic cells.Chemical inhibition of CENP-E in metaphase by syntelin prevented accurate central spindle assembly by perturbing temporal assembly of PRC1 to the midzone.Thus,CENP-E-mediated PRC1 assembly to the central spindle constitutes a temporal switch to organize dynamic kinetochore microtubules into stable midzone arrays.These findings reveal a previously uncharacterized role of CENP-E in temporal control of central spindle assembly.Since CENP-E is absent from yeast,we reasoned that metazoans evolved an elaborate central spindle organization machinery to ensure accurate sister chromatid segregation during anaphase and cytokinesis. | Xu Liu Leilei Xu Junying Li Phil Y.Yao Wanjuan Wang Hazrat Ismail Haowei Wang Bryce Liao Zhihong Yang Tarsha Ward Ke Ruan Jianchun Zhang Quan Wu Ping He Xia Ding Dongmei Wang Chuanhai Fu Zhen Dou Feng Yan Wenwen Wang Xing Liu Xuebiao Yao | 2020 | Journal of Molecular Cell Biology2020，12，8： | 3 |
| 19 | Kinetic investigation of in situ growth of CdMoO_4 nano-octahedra显示文摘CdMoO4 nano-octahedra were grown in situ at room temperature by reverse-microemulsion.Energy evolution from this growth process was monitored using microcalorimetry.The microcalorimetric heat flow (MCHF) curve showed a characteristic endothermic peak for the initial reaction,and double discontinuous exothermic peaks for the subsequent crystal growth.Combined with complementary characterization techniques,the evolution of morphology and size of the CdMoO4 nano-octahedra were correlated with the MCHF peaks.Calculations based on the microcalorimetric results at 298.15 K provided rate constants of 7.56×10–5 s–1 for the reaction and nucleation process and 1.59×10–4 s–1 for the crystallization process. | LI YanFen JIANG JunYing FAN GaoChao MA YuJie HUANG ZaiYin | 2011 | Chinese Science Bulletin2011，56，3： | 3 |
| 20 | Effects of dopant content on optical and electrical properties of In_2O_3: W transparent conductive films显示文摘The In 2 O 3 : W (IWO) films with different W content were deposited on glass substrate using direct current sputtering method. The structure, surface morphology, and optical and electrical properties were investigated. Results showed that both the carrier concentration and carrier mobility were increased with the doping of W. The IWO film with the lowest resistivity of 1.0×10 3 cm, highest carrier mobilityof 43.7 cm 2 V 1 s 1 and carrier concentration of 1.4×10 20 cm 3 was obtained at the content of 2.8 wt.%. The average optical transmittance from 300 nm to 900 nm reached 87.6%. | Zhang, Yuanpeng Li, Yuan Li, Chunzhi Wang, Wenwen Zhang, Junying Wang, Rongming | 2012 | Rare Metals2012，31，2： | 3 |
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