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| 1 | N6-Methyladenine DNA Methylation in Japonica and Indica Rice Genomes and Its Association with Gene Expression,Plant Development, and Stress Responses显示文摘N6-Methyladenine (6mA)DNA methylation has recently been implicated as a potential new epigenetic marker in eukaryotes,including the dioot modelArabidopsis thaliana.However,the conservation and divergence of 6mA distribution patterns and functions in plants remain elusive.Here we report high-quality 6mA methylomes at single-nucleotide resolution in rice based on substantially improved genome sequences of two rice cultivars,Nipponbare (Nip;Japonica)and 93-11 (Indica).Analysis of 6mA genomic distribution and its association with transcription suggest that 6mA distribution and function is rather conserved between rice and Arabidopsis.We found that 6mA levels are positively correlated with the expression of key stressrelated genes,which may be responsible for the difference in stress tolerance between Nip and 93-11. Moreover,we showed that mutations in DDM1 cause defects in plant growth and decreased 6mA level. Our results reveal that 6mA is a conserved DNA modification that is positively associated with gene expression and contributes to key agronomic traits in plants. | Qian Zhang Zhe Liang Xuean Cui Changmian Ji Yun Li Pingxian Zhang Jingrong Liu Adeel Riaz Pu Yao Min Liu Yunpeng Wang Tiegang Lu Hao Yu Donglei Yang Hongkun Zheng Xiaofeng Gu | 2018 | Molecular Plant2018，11，12： | 18 |
| 2 | Effect of acupuncture at Renying(ST 9) on gene expression profile of hypothalamus in spontaneously hypertensive rats显示文摘OBJECTIVE: To investigate changes in gene expression profiles in the hypothalamus related to the effects of acupuncture at the Renying(ST 9) acupoint in spontaneously hypertensive(SH) rats.METHODS: We randomly divided 18 SH rats into Renying(ST 9) group and model control group, 9 body weight-matched Wistar-Kyoto rats were used as blank controls. Acupuncture was performed manually for 20-min daily over 28 d in the Renying(ST 9) group. Rat Gene 2.0 array technology was used for the determination of gene expression profiles and the screened key genes were verified by real-time quantitative polymerase chain reaction(RT-PCR) analyses.RESULTS: The different groups exhibited differential gene expression: compared with the blank control group, 48 genes were up-regulated and 91 genes were down-regulated in the model group;compared with the model group, 79 genes were up-regulated and 80 genes were down-regulated in Renying(ST 9) group. The RT-PCR results of the key genes including Chi3 l1, Ephx2, Klk1, 5-HT1 A and Cbs were consistent with that of gene chip analysis.CONCLUTION: Acupuncture at Renying(ST 9)could significantly lower the blood pressure of SH rats and affect their hypothalamic gene expression profile. Genes associated with the contraction of vascular smooth muscle and the regulation of inflammation, neurotransmitters may be involved in acupuncture's antihypertensive mechanism. | Guo Yan Lu Juan Liang Jingrong Zhao Ruili Xu Jing Zhang Wei Park Kibeum Zhu Shipeng Chen Huan Ma Liangxiao | 2018 | Journal of Traditional Chinese Medicine2018，38，2： | 4 |
| 3 | Characteristics of SARS-CoV-2 Delta variant-infected individuals with intermittently positive retest viral RNA after discharge显示文摘SARS-CoV-2-infected patients will be discharged when viral ribonucleic acid(RNA)is consecutively negative at least twice over a 24-h interval.However,we have demonstrated that positive retest viral RNA,a particular clinical phenomenon initially observed in discharged SARS-CoV-2-infected patients in early 2020[1,2],is the occasional reoccurrence of Polymerase Chain Reaction(PCR)-detectable viral RNA but not reinfection[3]. | Lu Li Jingyan Tang Zhiwei Xie Qingxin Gan Guofang Tang Zhongwei Hu Huimin Zeng Jingrong Shi Jiaojiao Li Yan Li Changwen Ke Min Kang Dan Liang Huan Lu Yuwei Tong Xilong Deng Jinxin Liu Hongzhou Lu Fuxiang Wang Fengyu Hu Feng Li Nanshan Zhong Xiaoping Tang | 2022 | National Science Review2022，9，10： | 3 |
| 4 | CS1003, a novel human and mouse cross-reactive PD-1 monoclonal antibody for cancer therapy显示文摘The programmed cell death protein 1(PD-1)is an immune-checkpoint that negatively regulates the immune system and a key mechanism that tumors utilize to escape from immune surveillance.PD-1 antibodies can block the interaction of PD-1 with its ligands(PD-L1 and PD-L2),restore T cells activation,and elicit antitumor activity.In this paper,we reported a novel PD-1 monoclonal antibody(mAb)CS1003,which is a humanized IgG4 PD-1 mAb generated by conventional hybridoma technology,and currently being developed in multiple clinical trials as monotherapy or in combination with other anticancer agents.We showed that CS1003 bound to recombinant human,cynomolgus monkey,and mouse PD-1 with EC50 values of 0.1757,0.2459,and 0.3664 nM,respectively.CS1003 blocked PD-1 interaction with its ligands,dose-dependently enhanced T cell proliferation and secretion of cytokines(IL-2 and IFN-γ)to the levels comparable to the reference antibody pembrolizumab.Intraperitoneal administration of CS1003(0.1,0.5,2.5 mg/kg,once every 3 days)dose-dependently suppressed the growth of MC38-hPD-L1 colon cancer in hPD-1 knock-in mice.Pharmacokinetics(PK)study revealed a linear PK profile within the dose range of 2–18 mg/kg following single intravenous administration in cynomolgus monkey.These data provide a comprehensive preclinical characterization of CS1003 that supports its clinical development for cancer immunotherapy. | Fu Li Jingrong Li Ke Yin Juan Zhang Zhen-hu Li Liang Lu Yuan-wu Bao Zhen Qin Yong Zheng Bao-tian Yang Jing Li Xinzhong Wang | 2021 | Acta Pharmacologica Sinica2021，42，1： | 2 |
| 5 | Passivity-based Active Stabilization for DC Microgrid Applications显示文摘In this paper,an improved active stabilization strategy of the interface converters in microgrid applications is proposed on the basis of the passivity-based stability criterion(PBSC).As a critical part of AC and DC hybrid microgrids,the DC microgrid is taken as an example.In particular,a stabilization method with a proportional-integral(PI)controller and firstorder high-pass filter(HPF)is proposed to meet the passivity requirements of the overall control diagram with respect to the output voltage.Meanwhile,an output current feedback control loop is introduced to ensure the output impedance passivity.Moreover,a small-signal model of the parallel interface converter system is established to comprehensively study the influence of control parameters on the passivity of the converters.Based on the active stabilization method proposed in this study,by manipulating the control diagram of each interface converter,the passivity and stability of the DC microgrids with variable configuration can be guaranteed.Therefore,a generic and simplified design approach is realized.A simulation model with three interface converters is implemented in MATLAB/Simulink,and the effectiveness of the proposed passivity-based active stabilization algorithm is verified by using this simulation model. | Jiaqi Yu Jingrong Yu Yue Wang Yijia Cao Xiaonan Lu Dongbo Zhao | 2018 | CSEE Journal of Power and Energy Systems2018，4，1： | 2 |
| 6 | 显示文摘 | Lu Jingrong Chen Xiangfeng Lu Wei | 2006 | European Polymer Journal2006，42，5： | 1 |
| 7 | TLC Identification of Yao Medicine Pileostegia tomentellal and Extraction Technology and Content Determination of Umbelliferone显示文摘[Objectives]To establish a TLC and content determination method of Pileostegia tomentellal,with umbelliferone as the indicator component.[Methods]TLC identification was performed by silica gel G thin layer plate with n-hexane-ethyl acetate(4:3)as the developing agent,and the plate was examined by UV lamp(365 nm).The umbelliferone content was determined by HPLC:Inertsil ODS-3 C 18 column(4.60 mm×250 mm,5μm);mobile phase acetonitrile-0.2%phosphoric acid gradient elution;detection wavelength 320 nm,flow rate 1.0 mL/min,column temperature 30℃,injection volume 10μL.[Results]The chromatogram of P.tomentellal showed the same color spot in the same position as that of reference medicinal material,and the spot was clear with good specificity.Umbelliferone showed a good linear relationship when the injection volume was 2.63-131.27μg/mL(R^(2)=0.9997).The average recovery of umbelliferone in the low,middle and high adding groups of P.tomentellal was 99.57%and the RSD was 2.15%.[Conclusions]The method can effectively identify Yao medicine P.tomentellal and accurately determine the content of umbelliferone in medicinal materials,which will provide a scientific basis for the development and utilization of medicinal resources of Yao medicine P.tomentellal. | Jiangcun WEI Xiumei MA Meiyan QIU Bing QING Jingrong LU Hong LEI Xiaodong HUANG Wen ZHONG | 2024 | Medicinal Plant2024，15，2： | 0 |
| 8 | Effects of Different Drying Methods on Content of Paederosidic Acid in Paederia scandens显示文摘[Objectives]To establish a method to simultaneously determine the content of paederosidic acid in Paederia scandens dried by four different methods.[Methods]Reversed-phase high performance liquid chromatography(RP-HPLC)was adopted.The chromatographic column:Thermo SCIENTIFIC Hypersil GOLD Dim.(mm);mobile phase:acetonitrile-0.1%phosphoric acid aqueous solution;gradient elution;column temperature:30℃;flow rate:1 mL/min;detection wavelength:236 nm;injection volume:10μL.[Results]The linear range of the detection injection volume of paederosidic acid was 0.64-9.60μg(R=0.9992);the limit of quantity(LOQ)was 5.10 ng,and the limit of detection(LOD)was 1.36 ng;the RSD of the precision,stability and reproducibility test was all less than 3%;the sample recovery rate was 98.87%,RSD<3.00,n=6.The results show that the content of paederosidic acid in the shade-dried P.scandens is the highest,and the order of the content is P.scandens(dry in shade)>P.scandens(dried at 50℃)>P.scandens(dried at 60℃)>P.scandens(dried at 70℃).[Conclusions]This method is sensitive,reliable and reproducible,and can be used to simultaneously determine the content of paederosidic acid in P.scandens dried by four different methods. | Jingrong LU Haisheng ZENG Silu HE Xiumei MA Dongmei HUANG Guilin YANG Chengtong LIU Qiji ZHOU | 2020 | Medicinal Plant2020，11，6： | 0 |
| 9 | Thin-layer Identification,Extraction Process and Content Determination of Chlorogenic Acid in Laggera alata(D.Don)Sch.Bip.ex Oliv.显示文摘[Objectives]This paper aims to establish thin-layer identification and content determination method for Laggera alata(D.Don)Sch.Bip.ex Oliv.with chlorogenic acid as the index component and compare the content of chlorogenic acid in L.alata from different places in Guangxi.[Methods]Silica gel GF254 thin-layer plate was used for identification under an ultraviolet lamp(365 nm),with butyl acetate-formic acid-water(V∶V∶V=7∶2.5∶2.5)as a developing agent.The content of chlorogenic acid was determined under the following chromatographic conditions:column,Inertsil ODS-3 C18 column(4.60 mm×250 mm,5μm);mobile phase,methanol-0.1%phosphoric acid(28∶72);detection wavelength,329 nm;flow rate,1.0 mL/min;column temperature,25℃;and injection volume,10μL.[Results]Chlorogenic acid can be detected by thin layer chromatography with clear spot and good specificity.Chlorogenic acid showed a good linear relationship in the injection amount range of 0.099-0.99μg(R^(2)=0.9999).The content of chlorogenic acid in L.alata varied greatly among the 10 different producing areas in Guangxi.L.alata produced in Dee Township,Longlin,Baise,Guangxi showed the highest chlorogenic acid content,and that produced in Shangsi County and Pingle County showed the lowest chlorogenic acid content.[Conclusions]This method can effectively identify L.alata and accurately determine the content of chlorogenic acid,thereby providing a scientific basis for the development and utilization of L.alata resources. | Qiaofeng QIN Xue JIANG Jiangcun WEI Jiabao MA Jingrong LU Lifu WEI Xiumei MA Xuanyang ZENG | 2020 | Medicinal Plant2020，11，6： | 0 |
| 10 | Analysis and Study on Quality Control of Yao Medicine Damnacanthus macrophyllus Based on Methods in Chinese Pharmacopoeia显示文摘[Objectives]To establish a quality analysis method for Yao medicine Damnacanthus macrophyllus Sieb.ex Miq.var.giganteus(Makino)Koidz..[D.indicus Gaertn.f.var.giganteus Makino].[Methods]The moisture,total ash and alcohol extracts of D.macrophyllus medicinal materials were determined according to the experimental methods in the Chinese Pharmacopoeia(2020).[Results]All the 10 batches of D.macrophyllus medicinal materials from different collection times and different regions in Guangxi had the same plant morphological characteristics.The experimental results indicate that the moisture was 9.81%-11.52%,the total ash content was 7.37%-9.53%,the acid-insoluble ash content was 1.10%-1.97%,and the extract was 8.25%-10.22%.[Conclusions]This study provides a scientific basis for establishing a quality standard for Yao medicine D.macrophyllus medicinal materials,and it is expected to better utilize and develop D.macrophyllus. | Jiangcun WEI Xiumei MA Yanxiu WANG Bihua NONG Chunli TANG Jingrong LU Fengxian ZHAO | 2021 | Medicinal Plant2021，12，2： | 0 |
| 11 | Research Progress and Application Prospect of Chengyin Decoction (Granule)显示文摘Research and development of Chengyin detoxification prescription are taken as research objects. Starting from preparation research and development, clinical research, urgency and market demand, and significance for industrial development, the relevant research of Chengyin detoxification prescription is systematically reviewed, and the application prospect of the ethnic medicine preparations is looked forward to. | Qingmei DENG Jiangcun WEI Xiumei MA Jingrong LU Xianyi SHI Yinghong HUANG Bing QING Wen ZHONG Zujie QIN | 2023 | Medicinal Plant2023，14，4： | 0 |
| 12 | Retrospective Analysis of the Duration of Hospitalization of Imported Patients Infected with SARS-CoV-2 in Guangzhou, 2021显示文摘The pandemic of coronavirus disease 2019 is“not over,”in fact,the“dynamic clearing”policy for SARS-CoV-2 control and prevention in China has been firmly enforced.This study aimed to analyze the clinical symptoms and dynamic viral RNA changes in 2021 at Guangzhou Eighth People’s Hospital.This study showed that 31.4%of the patients(695/2212)tested negative for viral RNA from admission to the final release from quarantine.Of all negative cases,86.5%(601/695)remained in the hospital for no more than 5 days and were asymptomatic or mild.Among the remaining 402 patients who stayed for no more than 5 days,76.4%(307/402)were viral RNA retest positive during the isolation stage.However,96.4%of the peak viral RNA(296/307)was over Ct=33 cycles during the isolation stage. | Zhiwei Xie Guofang Tang Lu Li Jingrong Shi Qingxin Gan Xiaowen Zheng Jingyan Tang Huimin Zeng Chuyu Zhang Sisi Cheni Jianping Cui Zishi Lin Lihua Lin Youxia Li Jinxin Liu Feng Li Fengyu Hui Xiaoping Tang Xilong Deng | 2023 | Infectious Diseases & Immunity2023，3，1： | 0 |
| 13 | Extraction Technology and Content Determination of Total Flavonoids from Traditional Chinese Medicine Yipichou显示文摘[Objectives]The research aimed to optimize extraction technology of total flavonoids from traditional Chinese medicine Yipichou,and establish methods of extraction and content determination of its medicinal materials.[Methods]Using reflux extraction method,total flavonoids of Yipichou were extracted.Via single-factor test,influence of extraction method,extraction solvent concentration,extraction volume and extraction time on content of total flavonoids from Yipichou was inspected.Using orthogonal test,extraction conditions were optimized,and optimal extraction technology of total flavonoids from Yipichou was obtained.[Results]The optimal extraction process of total flavonoids from Yipichou was as below:70%of methanol,1∶40 of solid-liquid ratio,and 1.0 h of reflux extraction time.[Conclusions]In the test,total flavonoids were extracted from Yipichou,with better repeatability,and stable and feasible method,which could provide certain scientific basis for studying quality standard of this medicinal material. | Qianqian LIU Wen ZHONG Zongxi SUN Xiumei MA Xinli LU Yusheng HUANG Jingrong LU Jiangcun WEI | 2021 | Medicinal Plant2021，12，1： | 0 |
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