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| 1 | The Tea Tree Genome Provides Insights into Tea Flavor and Independent Evolution of Caffeine Biosynthesis显示文摘茶是世界有巨大的经济、药用、文化的重要性的最旧、很流行的包含咖啡因的饮料。这里,我们在场第一个高质量的核苷酸序列充满重复(80.9%) ,栽培的茶树山茶 sinensis 的 3.02-Gb 染色体。我们证明茶树的一种非常地大的染色体尺寸从一些 LTR retrotransposon 家庭的慢、稳定、长期的扩大被结果。除了一个最近的整个染色体的复制事件,基因的系特定的扩大把新陈代谢的生合成与 flavonoid 联系了被发现,它提高 catechin 生产,萜烯酶激活,和应力公差,为茶风味和改编的重要特征。我们相对可可子和咖啡表明一个独立人士和茶咖啡因合成小径的快速的进化。在 25 山茶种类之中的比较研究表明那更高的表情大多数 flavonoid- 和咖啡因铺平 -- 然而并非 theanine 相关的基因贡献 catechins 和咖啡因的增加的生产并且因此提高处理茶的适用性和茶质量。这些新奇调查结果为进一步的 metabolomic 和典型生合成小径的功能的 genomic 精炼铺平道路并且将帮助开发将最后满足并且吸引世界范围的更多的茶喝酒者的茶风味的一个更多样化的集合。 | En-Hua Xia Hai-Bin Zhang Jun Sheng Kui Li Qun-Jie Zhang Changhoon Kim Yun Zhang Yuan Liu Ting Zhu Wei Li Hui Huang Yan Tong Hong Nan Cong Shi Chao Shi Jian-Jun Jiang Shu-Yan Mao Jun-Ying Jiao Dan Zhang Yuan Zhao You-Jie Zhao Li-Ping Zhang Yun-Long Liu Ben-Ying Liu Yue Yu Sheng-Fu Shao De-Jiang Ni Evan E. Eichler Li-Zhi Gao | 2017 | Molecular Plant2017，10，6： | 113 |
| 2 | Prevalence of minimal hepatic encephalopathy and quality of life evaluations in hospitalized cirrhotic patients in China显示文摘AIM:To investigate the prevalence of minimal hepatic encephalopathy(MHE)and to assess corresponding health-related quality of life(HRQoL)in hospitalized cirrhotic patients in China.METHODS:This multi-center cross-sectional study included 16 teaching hospitals,which were members of 'Hepatobiliary Cooperation Group,Society of Gastroenterology,Chinese Medical Association',from different areas of China carried out between June and October in 2011.All the eligible hospitalized cirrhotic patients(n = 538)were required to complete triplicate number connection tests combined with one digit symbol test for diagnosing MHE.Patients' clinical examination data were complemented by a modified questionnaire assessing HRQoL.Written informed consent was obtained from each patient.RESULTS:Male was predominant(68.6%)in 519 patients who met the criteria of the study,with a mean age of 49.17 ± 11.02 years.The most common cause of liver cirrhosis was chronic hepatitis B(55.9%).The prevalence of MHE was 39.9% and varied by ChildPugh-Classification score(CPC-A:24.8%,CPC-B:39.4% and CPC-C:56.1%,P < 0.01).MHE(P < 0.01)and higher CPC scores(P < 0.01)were associated with a high HRQoL scores(reflecting poorer quality of life).The prevalence of MHE was proportionate to CPC(P = 0.01)and high quality of life scores(P = 0.01).CONCLUSION:Hospitalized cirrhotic patients have a high prevalence of MHE that is proportionate to the degree of liver function and HRQoL impairment. | Ji-Yao Wang Ning-Ping Zhang Bao-Rong Chi Yu-Qing Mi Li-Na Meng Ying-Di Liu Jiang-Bin Wang Hai-Xing Jiang Jin-Hui Yang Yun Xu Xiao Li Jian-Ming Xu Guo Zhang Xin-Min Zhou Yu-Zheng Zhuge De-An Tian Jin Ye Yu-Lan Liu | 2013 | World Journal of Gastroenterology2013，19，30： | 64 |
| 3 | Clinical characteristics, diagnosis and management of respiratory distress syndrome in full-term neonates显示文摘然而,背景呼吸悲痛症候群(RDS ) 是新生的呼吸失败和新生的死亡的最普通的原因之一它的临床的特征与早熟的 RDS,和这些特征很不同很好没迄今为止被记录。这研究是在完整术语的出生不满一月的婴儿调查致病,临床的特征和 RDS 的管理策略,与开发为改进在有 RDS.Methods 的完整术语的出生不满一月的婴儿的结果的一个工作协议的目的有 RDS 的 125 个完整术语的婴儿的一个总数在这研究被注册。他们的临床并且实验室数据为分析 125 个案例包括了的完整术语的新生的 RDS.Results (1 ) 的特征被收集 94 男性和 31 个女婴儿,阴道交货在 45 种情况中发生在 80 种情况和剖腹产中。(2 ) RDS 的发作时间是(3.11 牧潯敶吗? | LIU Jing SHI Yun DONG Jian-ying ZHENG Tian LI Jing-ya LU Li-li LIU Jing-jing LIANG Jing ZHANG Hao FENG Zhi-chun | 2010 | Chinese Medical Journal2010，，19： | 79 |
| 4 | Diagnostic value of glypican-3 in serum and liver for primary hepatocellular carcinoma显示文摘AIM:To evaluate the diagnostic value of glypican-3(GPC3) in serum and liver for primary hepatocellular carcinoma(HCC).METHODS:Serum levels of GPC3 and α-fetoprotein(AFP) were measured in 75 patients with primary HCC and 32 patients with liver cirrhosis.Expression of GPC3 and AFP in 58 HCC and 12 cirrhotic specimens was detected with immunohistochemical staining.RESULTS:When the cut-off value of serum GPC3 was set at 300 ng/L,its sensitivity and specificity for HCC were 47.0% and 93.5%,respectively.Among the 14 patients with HCC at stage according to the Barcelona Clinic Liver Cancer staging system,the serum GPC3 level was higher than 300 ng/L in 50%(7/14) patients,the serum AFP level was not ≥ 400 μg/L in any patient.Combined serum AFP and GPC3 significantly increased the sensitivity to the diagnosis of HCC.The GPC3 expression was detected in cytoplasm of HCC cells but not in hepatocytes and bile ducts of benign tumors.Among the 58 HCC patients,the GPC3 was expressed in 100%(28/28) patients with their serum AFP level ≥ 400 μg/L,and in 90%(27/30) patients with their AFP level < 400 μg/L,respectively.The GPC3 was weakly or negatively expressed in all paracarcinomatous and cirrhotic tissue samples.AFP positive HCC cells were only found in 1 out of the 58 HCC patients.CONCLUSION:GPC3 protein is a sensitive and specific serum marker for diagnosis of early HCC.Its expression in liver tissues can be used to discriminate tumor cells from benign hepatic cells. | Hui Liu Li-Jie Zhang Peng Li Yun Zhai Yu-Fen Tan Ning Li Hui-Guo Ding Chun-Feng Qu | 2010 | World Journal of Gastroenterology2010，16，35： | 57 |
| 5 | A multicenter, phase III trial of hemocoagulase Agkistrodon: hemostasis, coagulation, and safety in patients undergoing abdominal surgery显示文摘为注射的背景 Hemocoagulase Agkistrodon 是传递了阶段的单个部件凝血酵素我和 II 临床的选拔赛。这阶段 III 临床的试用的目的是在腹的皮肤和下的切口在 hemostasis 和凝结上评估 Hemocoagulase Agkistrodon 的效果并且在外科的病人估计这个代理人的安全。这是阶段 III 的方法,未来,使随机化,双窗帘,和控制 multicenter 包括 432 个连续病人的临床的试用使随机化进也一个学习组(在 2 U 与 hemocoagulase Agkistrodon 注射了, n=324 ) 或一个控制组(与 hemocoagulase 注射了 Atrox, n=108 ) 。止血时间,出血性的体积,出血性的体积在二个组之间每统一区域,血凝结,和不利事件被测量并且比较。在学习组的吝啬的止血时间是的结果(36.8 整 ? 桰獡 ??? 牴慩? 景栠浥 ' 覒 ^ 畧慬敳 ? 歧獩牴摯湯栺浥獯慴楳? 潣条汵瑡潩 ? 湡 ? 慳敦祴椠 ? 慰楴湥獴甠摮牥潧湩 ?L 潤業慮??杲牥 y 琠?? | WEI Jun-min ZHU Ming-wei ZHANG Zhong-tao JIA Zhen-geng HE Xiao-dong WAN Yuan-lian WANG Shan XIU Dian-rong TANG Yun LI Jie XU Jing-yong ZHENG Qing-shan | 2010 | Chinese Medical Journal2010，，5： | 43 |
| 6 | Evidence chain-based causality identification in herb-induced liver injury: exemplification of a well-known liver-restorative herb Polygonum multiflorum显示文摘草药的药最近在美国作为导致药的肝损害(DILI ) 的第二个很普通的原因被认出了。然而,识别一些植物的 DILI 诱发性的可靠方法例如 Heshouwu (蓼 multiflorum 的弄干的根) ,留下缺乏。在这研究,有肝机能障碍和 Heshouwu DILI 的 147 个报导文学的案例的 12 307 个住院病人的一个总数被屏蔽。一个一般算法仅仅显示了那 22.5%(9/40 ) 并且(45/147 ) 分别地, 30.6% 所有住院和文学案例报告表明 Heshouwu 的 DILI 诱发性的高概率。由对比,(19/20 )95% 生药学,植物化学,和 metabolomic 有希望地调查的所有情况测试展出高度可能的诱发性,包括不正确地以前被归因的一个病人和从由 pharmacognostic 证据的 Heshouwu 诱发性被排除的一个案例。污染也是的毒素(重金属,杀虫剂,和 mycotoxins ) 从 Heshouwu DILI 诱发性排除了。为 Heshouwu DILI 诊断地址安全的这些屏蔽方法的客观性担心考虑包含芪的草药的药和饮食的补充。 | Jiabo Wang Zhijie Ma Ming Niu Yun Zhu Qingsheng Liang Yanling Zhao Jingyuan Song Zhaofang Bai Yaming Zhang Ping Zhang Na Li Yakun Meng Qi Li Lushan Qin Guangju Teng Junling Cao Baosen Li Shilin Chen Yonggang Li Zhengsheng Zou Honghao Zhou Xiaohe Xiao | 2015 | Frontiers of Medicine2015，9，4： | 47 |
| 7 | Screening for main components associated with the idiosyncratic hepatotoxicity of a tonic herb, Polygonum multiflorum显示文摘典型药用的植物的主要成分,蓼 multiflorum (用汉语的 Heshouwu ) ,那导致特质的肝损害仍然保持不清楚。我们的以前的工作证明了有 lipopolysaccharide (LPS ) 的无毒的剂量和 Heshouwu 的治疗学的剂量的 cotreatment 能在老鼠导致肝损害,而独唱治疗不能导致看得见的损害。在现在的工作,用成分大美人和敲门在里面策略,我们发现 Heshouwu 的乙醇醋酸盐(EA ) 摘录在对待 LPS 的老鼠显示了可比较的特质的 hepatotoxicity 到整个摘录。结果显示血浆丙氨酸 aminotransferase, aspartate aminotransferase,和肝 histologic 的重要举起变化,而另外的分开的部分没能导致肝损害。有另外的分开的部分的 EA 摘录的混合在对待 LPS 的老鼠导致了可比较的特质的 hepatotoxicity 到整个摘录。化学分析进一步揭示了那 2,3,5,4-tetrahydroxy trans-stilbene-2-O -- 配糖物(trans-SG ) 和它的 cis 异构体是在 EA 的二主要混合物摘录。而且,孤立的 cis- ,并且不它的 trans 异构体,显示的可比较的特质的 hepatotoxicity 到在对待 LPS 的老鼠的 EA 摘录。cis-SG 的更高的内容从与收集的将军相比与 Heshouwu 联系的实际的肝沉醉病人在 Heshouwu 白酒或准备被检测样品。另外,血浆 metabolomics 分析证明 cis-SG-disturbing 充实显著地不同于的小径在对待 LPS 的老鼠的 trans-SG。所有这些结果建议 cis-SG 仔细与 Heshouwu 的特质的 hepatotoxicity 被联系。认为 trans-SG 的 cis-trans 异构化被紫外光或日光调停,我们的调查结果为在药发现控制 photoisomerization 并且为 Heshouwu 和芪相关的药的临床的使用用作参考。 | Chunyu Li Ming Niu Zhaofang Bai Congen Zhang Yanling Zhao Ruiyu Li Can Tu Huifang Li Jing Jing Yakun Meng Zhijie Ma Wuwen Feng Jinfa Tang Yun Zhu Jinjie Li Xiaoya Shang Zhengsheng Zou Xiaohe Xiao Jiabo Wang | 2017 | Frontiers of Medicine2017，11，2： | 41 |
| 8 | Changes in circulating Foxp3^+ regulatory T cells and interleukin-17-producing T helper cells during HBV-related acute-on-chronic liver failure显示文摘AIM:To longitudinally investigate cytokine gene expression and protein levels in Th17 and Treg cells,to observe T-cell phenotypes during hepatitis B virus(HBV)-related acute-on-chronic liver failure(ACHBLF)and to analyze changes in Th17 and Treg phenotypes during disease progression.METHODS:We measured the expression of seven Th17/Treg differentiation-related genes and serum concentrations of the corresponding cytokines in 18ACHBLF,18 chronic hepatitis B(CHB)disease controls and 10 healthy controls(HCs)by real-time quantitative pCR and enzyme linked immunosorbent assay.peripheral Th17 and Treg cell frequencies were analyzed by flow cytometry.RESULTS:From the onset of ACHBLF,patients presented with a conductive Th17 differentiation cytokine environment accompanied by high Th17 frequency and high serum IL-17 levels,which were sustained throughout the disease course.The Treg-related cytokine IL-2and Foxp3 were also up-regulated from disease onset,and Foxp3 gene expression showed a gradually increasing trend during ACHBLF.The circular phenotype of Treg and Th17 cells showed changes from the onset of ACHGLF.At disease onset,Th17 frequency increased significantly compared with both CHB and HCs,but Treg cell frequency decreased significantly compared with CHB.During the ACHBLF event,Th17 frequency remained higher compared with HCs,but decreased sharply from the peak point to the recovery point;Treg cell frequency increased gradually during the ACHBLF event.Treg and Th17 cell counts correlated with ACHBLF development;in all patients,serum IL-17 levels significantly correlated with patient serum ALT levels.In survivors,Th17 frequency at the onset point and the Treg to Th17 ratio at the peak point correlated with the patient’s model for end stage liver disease(MELD)plus sodium(MELD-Na)score.The Treg to Th17 ratio and the Th17 frequency at onset were significant predictors of patient survival.Low Treg/Th17 cell ratios at the onset predicted poor survival.Survivors exhibited an initial decrease in the circulating Treg/Th17 ratio from the onset to the peak time,and subsequently displayed a continuous increase.CONCLUSION:Treg and Th17 cells showed changes in genes,protein levels and T cell phenotypes during ACHBLF events.An increased Treg/Th17 ratio was associated with the survival of ACHBLF patients. | Xue-Song Liang Cheng-Zhong Li Yin Zhou Wei Yin Ya Yun Liu Wen-Han Fan | 2014 | World Journal of Gastroenterology2014，20，26： | 44 |
| 9 | Field experiment on a robust hierarchical metropolitan quantum cryptography network显示文摘A hierarchical metropolitan quantum cryptography network upon the inner-city commercial telecom fiber cables is reported in this paper. The seven-user network contains a four-node backbone net with one node acting as the subnet gateway, a two-user subnet and a single-fiber access link, which is realized by the Faraday-Michelson interferometer set-ups. The techniques of the quantum router, optical switch and trusted relay are assembled here to guarantee the feasibility and expandability of the quantum cryptography network. Five nodes of the network are located in the government departments and the secure keys generated by the quantum key distribution network are utilized to encrypt the instant video, sound, text messages and confidential files transmitting between these bureaus. The whole implementation including the hierarchical quantum cryptographic communication network links and the corresponding application software shows a big step toward the practical user-oriented network with a high security level. | XU FangXing CHEN Wei WANG Shuang YIN ZhenQiang ZHANG Yang LIU Yun ZHOU Zheng ZHAO YiBo LI HongWei LIU Dong HAN ZhengFu GUO GuangCan | 2009 | Chinese Science Bulletin2009，54，17： | 40 |
| 10 | Transplantation of collagen scaffold with autologous bone marrow mononuclear cells promotes functional endometrium reconstruction via downregulating ΔNp63 expression in Asherman's syndrome显示文摘Asherman's syndrome(AS) is a common disease that presents endometrial regeneration disorder. However, little is known about its molecular features of this aregenerative endometrium in AS and how to reconstruct the functioning endometrium for the patients with AS. Here, we report that ΔNp63 is significantly upregulated in residual epithelial cells of the impaired endometrium in AS; the upregulated-ΔNp63 induces endometrial quiescence and alteration of stemness. Importantly, we demonstrate that engrafting high density of autologous bone marrow mononuclear cells(BMNCs) loaded in collagen scaffold onto the uterine lining of patients with AS downregulates ΔNp63 expression, reverses ΔNp63-induced pathological changes, normalizes the stemness alterations and restores endometrial regeneration. Finally, five patients achieved successful pregnancies and live births. Therefore, we conclude that ΔNp63 is a crucial therapeutic target for AS. This novel treatment significantly improves the outcome for the patients with severe AS. | Guangfeng Zhao Yun Cao Xianghong Zhu Xiaoqiu Tang Lijun Ding Haixiang Sun Juan Li Xinan Li Chenyan Dai Tong Ru Hui Zhu Jingjie Lu Caimei Lin Jingmei Wang Guijun Yan Huiyan Wang Lei Wang Yimin Dai Bin Wang Ruotian Li Jianwu Dai Yan Zhou Yali Hu | 2017 | Science China(Life Sciences)2017，60，4： | 39 |
| 11 | Linking atmospheric pollution to cryospheric change in the Third Pole region: current progress and future prospects显示文摘The Tibetan Plateau and its surroundings are known as the Third Pole(TP). This region is noted for its high rates of glacier melt and the associated hydrological shifts that affect water supplies in Asia. Atmospheric pollutants contribute to climatic and cryospheric changes through their effects on solar radiation and the albedos of snow and ice surfaces; moreover, the behavior and fates within the cryosphere and environmental impacts of environmental pollutants are topics of increasing concern. In this review, we introduce a coordinated monitoring and research framework and network to link atmospheric pollution and cryospheric changes(APCC) within the TP region. We then provide an up-to-date summary of progress and achievements related to the APCC research framework, including aspects of atmospheric pollution’s composition and concentration, spatial and temporal variations, trans-boundary transport pathways and mechanisms, and effects on the warming of atmosphere and changing in Indian monsoon, as well as melting of glacier and snow cover. We highlight that exogenous air pollutants can enter into the TP’s environments and cause great impacts on regional climatic and environmental changes. At last, we propose future research priorities and map out an extended program at the global scale. The ongoing monitoring activities and research facilitate comprehensive studies of atmosphere–cryosphere interactions, represent one of China’s key research expeditions to the TP and the polar regions and contribute to the global perspective of earth system science. | Shichang Kang Qianggong Zhang Yun Qian Zhenming Ji Chaoliu Li Zhiyuan Cong Yulan Zhang Junming Guo Wentao Du Jie Huang Qinglong You Arnico K.Panday Maheswar Rupakheti Deliang Chen Orjan Gustafsson Mark H.Thiemens Dahe Qin | 2019 | National Science Review2019，6，4： | 35 |
| 12 | Chinese Stroke Association guidelines for clinical management of cerebrovascular disorders:executive summary and 2019 update of clinical management of stroke rehabilitation显示文摘Stroke is the third leading cause of long-term disability.Effective stroke rehabilitation should be provided to patients with a moderate or severe stroke so as to achieve functional recovery.Also,stroke rehabilitation is moving towards more intense therapy models that incorporate technologies such as robotics and computer games.Evidence-based guidelines are useful for promoting the best possible clinical practices.This guideline summarises the modes of stroke rehabilitation in China-three-stage rehabilitation network,rehabilitation of stroke dysfunction,training for activities of daily living,stroke complications and rehabilitation care.The aim of this guideline was to provide a synopsis of best clinical practices in the stroke rehabilitation of adult patients in China. | Tong Zhang Jun Zhao Xueping Li Yulong Bai Baojun Wang Yun Qu Bingjie Li Shengjie Zhao Chinese Stroke Association Stroke Council Guideline Writing Committee | 2020 | Stroke & Vascular Neurology2020，5，3： | 38 |
| 13 | Multi-energy Storage System Model Based on Electricity Heat and Hydrogen Coordinated Optimization for Power Grid Flexibility显示文摘Based on decreasing the flexibility of the power grid through the integration of large-scale renewable energy,a multi-energy storage system architectural model and its coor-dination operational strategy with the same flexibility as in the pumped storage power station and battery energy storage system(BESS)are studied.According to the new energy fluctuation characteristics and the different peak valley parameters in the power grid,this paper proposes a electricity heat hydrogen multi-energy storage system(EHH-MESS)and its coordination and optimization operational model to reduce the curtailment of wind power and photovoltaic(PV)to the power grid and improve the flexibility of the power grid.Finally,this paper studied the simulation model of an energy storage optimization control strategy after the multi-energy storage system is connected to the distribution networks,and analyzed three operational modes of the multi-energy storage system.The simulation results show that the EHH-MESS proposed in this paper has a better power grid regulation flexibility and economy,and can be used to replace the battery energy storage system based on MATLAB. | Yun Teng Zedi Wang Yan Li Qian Ma Qian Hui Shubin Li | 2019 | CSEE Journal of Power and Energy Systems2019，5，2： | 38 |
| 14 | Overtopping breaching of cohesive homogeneous earth dam with different cohesive strength显示文摘In consideration of the range of clay content of Chinese earth dams, the world's highest prototype tests have been made to research on the effects of cohesive strength of filling of cohesive homogeneous earth dam on breach formation. Three breach mechanisms were presented, they were the source-tracing erosion of dam body with the form of 'multilevel headcut', 'two-helix flow' erosion of dam crest and collapse of breach sidewalls due to instability. It can be concluded that the cohesive strength of filling of earth dam has great effect on breach formation. When the cohesive strength is bigger, the breach process becomes slower, and the peak outflow and the final width and depth of breach become smaller. The main character of the breach formation is head cutting and dumping collapse. When the cohesive strength is smaller, the breach process becomes faster, and the peak outflow, the final width and depth of breach become bigger. The main character of the breach formation is single level head cutting and shearing collapse. | ZHANG JianYun LI Yun XUAN GuoXiang WANG XiaoGang LI Jun | 2009 | Science China(Technological Sciences)2009，52，10： | 34 |
| 15 | Prevalence and evolution of drug resistance HIV-1 variants in Henan, China显示文摘To understand the prevalence and evolution of drug resistant HIV strains in Henan China after the implementation of free antiretroviral therapy for AIDS patients. 45 drug nave AIDS patients, 118 AIDS patients who received three months antiretroviral therapy and 124 AIDS patients who received six months antiretroviral treatment were recruited in the southern part of Henan province. Information on general condition, antiretroviral medicines, adherence and clinical syndromes were collected by face to face interview. Meanwhile, 14ml EDTA anticoagulant blood was drawn. CD4/CD8 T cell count, viral load and genotypic drug resistance were tested. The rates of clinical improvement were 55.1% and 50.8% respectively three months and six months after antiretroviral therapy. The mean CD4 cell count after antiretroviral therapy was significantly higher than in drug nave patients. The prevalence rate of drug resistant HIV strains were 13. 9%, 45.4% and 62.7% in drug nave patients, three month treatment patients and six month treatment patients, respectively. The number of resistance mutation codons and the frequency of mutations increased significantly with continued antiretroviral therapy. The mutation sites were primarily at the 103, 106 and 215 codons in the three-month treatment group and they increased to 15 codon mutations in the six-month treatment group. From this result, the evolution of drug resistant strains was inferred to begin with the high level NNRTI resistant strain, and then develop low level resistant strains to NRTIs. The HIV strains with high level resistance to NVP and low level resistance to AZT and DDI were highly prevalent because of the AZT+DDI+NVP combination therapy. These HIV strains were also cross resistant to DLV, EFV, DDC and D4T. Poor adherence to therapy was believed to be the main reason for the emergence and prevalence of drug resistant HIV strains. The prevalence of drug resistant HIV strains was increased with the continu- ation of antiretroviral therapy in the southern part of Henan province. Measures, that could promote high level adherence, provide new drugs and change ART regimens in failing patients, should be implemented as soon as possible. | Jing Yun LI Han Ping LI Lin LI Hong LI Zhe WANG Kun YANG Zuo Yi BAO Dao Min ZHUANG Si Yang LIU Yong Jian LIU Hui XING Yi Ming SHAO | 2005 | Cell Research2005，15，11： | 34 |
| 16 | Inhibition of CXCR4 activity with AMD3100 decreases invasion of human colorectal cancer cells in vitro显示文摘AIM: To investigate the effect and mechanism of blockade of the CXC chemokine receptor-4 (CXCR4) signaling pathway by AMD3100, a small non-peptide CXCR4 inhibitor, on invasion and metastasis of colorectal cancer cells in vitro. METHODS: Human colorectal cancer cell line SW480 was treated with AMD3100 at different final concentrations. 3-(4,5-dimethylthiazole-2-yl)-2.5-dipheny-ltetrazolium bromide (MTT) assay was used to detect the effect of AMD3100 on cell proliferation. The invasion ability of SW480 cells was determined by cell invasion assay kit. In the presence of AMD3100, the CXCL12-mediated migratory response of SW480 cells was tested by classical chemotaxis assays. RT-PCR analysis and Western blotting were used to detect the expression of vascular endothelial growth factor (VEGF), matrix metalloproteinase-2 (MMP-2) and -9 (MMP-9) in SW480 cells. RESULTS: Cell viability was significantly suppressed by AMD3100 in a dose-dependent manner. AMD3100 (100 and 1000 ng/mL) significantly inhibited the invasion ability of SW480 cells. Treatment with AMD3100 markedly reduced the expression of VEGF and MMP-9 but not MMP-2 in SW480 cells. CONCLUSION: The CXCL12/CXCR4 system is an important mediator of proliferation and invasion of CXCR4-expressing colorectal cancer cells. AMD3100 inhibited invasion and metastasis activity of the colorectalcancer cell line SW480 through down-regulation of VEGF and MMP-9 expression. | Ji-Kun Li Liang Yu Yun Shen Li-Sheng Zhou Yi-Cheng Wang Jian-Hai Zhang | 2008 | World Journal of Gastroenterology2008，14，15： | 34 |
| 17 | Dissection of genetic overlap of salt tolerance QTLs at the seedling and tillering stages using backcross introgression lines in rice显示文摘QTLs for salt-tolerance(ST)related traits at the seedling and tillering stages were identified using 99 BC2F8 introgression lines(IL)derived from a cross between IR64(indica)as a recurrent parent and Binam(japonica)from Iran as the donor parent.Thirteen QTLs affecting survival days of seedlings(SDS), score of salt toxicity of leaves(SST),shoot K + concentration(SKC)and shoot Na + concentration(SNC) at the seedling stage and 22 QTLs underlying fresh weight of shoots(FW),tiller number per plant(TN) and plant height(PH)at the tillering stage were identified.Most QTLs detected at the tillering stage showed obvious differential expression to salt stress and were classified into three types based on their differential behaviors.Type I included 11 QTLs which were expressed only under the non-stress condition.Type II included five QTLs expressed in the control and the salt stress conditions,and three of them(QPh5,QPh8 and QTn9)had similar quantity and the same direction of gene effect,suggesting their expression was less influenced by salt stress.Type III included six QTLs which were detectable only under salt stress,suggesting that these QTLs were apparently induced by the stress.Thirteen QTLs affecting trait difference or trait stability of ILs between the stress and non-stress conditions were identified and the Binam alleles at all loci except QPh4,QTn2 and QFw2a decreased trait difference.The three QTLs less influenced by the stress and 13 QTLs affecting trait stability were considered as ST QTLs which contributed to ST.Comparing the distribution of QTLs detected at the seedling and tillering stages,most(69%)of them were genetically independent.Only four were the same or adjacent regions on chromosomes 1,2,8 and 11 harboring ST QTLs detected at the two stages,suggesting that partial genetic overlap of ST across the two stages occurs.It is likely,therefore,to develop ST rice variety for both stages by pyramiding of ST QTLs of different stages or selection against the overlapping QTLs between the two stages via marker-assisted selection(MAS). | ZANG JinPing 1 ,SUN Yong 1 ,WANG Yun 1 ,YANG Jing 1 ,LI Fang 1 ,ZHOU YongLi 1 ,ZHU LingHua 1 , Reys JESSICA2,Fotokian MOHAMMADHOSEIN 2,XU JianLong 1&LI ZhiKang 1,2 1Institute of Crop Sciences/National Key Facility for Crop Gene Resources&Genetic Improvement,Chinese Academy of Agricul- tural Sciences,Beijing 100081,China 2International Rice Research Institute,DAPO Box 7777,Metro Manila,Philippines | 2008 | Science China(Life Sciences)2008，51，7： | 32 |
| 18 | 2000–2020年中国氮氧化物排放清单及排放趋势(英文)显示文摘研究目的:建立2000–2020年中国氮氧化物排放清单,了解中国主要行业和省份氮氧化物的排放情况,为评估氮氧化物的环境影响和制定相关减排政策提供依据。创新要点:分析了中国主要省份产业结构对氮氧化物排放量的影响;根据不同情景分析,预测2020年中国氮氧化物的排放量。研究方法:1.基于自底向上法,根据不同类型化石燃料的氮氧化物排放因子,结合化石燃料消耗量,建立中国2000–2010年氮氧化物排放清单;2.基于IPAT方程,并以中国2000–2010年的国内生产总值增长数据和氮氧化物排放量为依据,分三种情景,分析2011–2020年中国能源消耗和氮氧化物排放趋势。重要结论:2010年中国氮氧化物的排放量约是2000年的两倍;自2009年起,中国氮氧化物总排放量超过了二氧化硫总排放量;主要由于产业结构和地区生产总值的不同,中国东部和西部氮氧化物排放量有明显差异;制造业、电力行业和交通运输业是中国氮氧化物的主要排放源,其中交通运输业氮氧化物排放量呈现逐年增长趋势;预计2020年中国氮氧化物排放量为19.7 Mt。 | Yun SHI Yin-feng XIA Bi-hong LU Nan LIU Lei ZHANG Su-jing LI Wei LI | 2014 | Journal of Zhejiang University-Science A(Applied Physics & Engineering)2014，15，6： | 34 |
| 19 | Effects of large dose of dexamethasone on inflammatory mediators and pancreatic cell apoptosis of rats with severe acute pancreatitis显示文摘AIM: To investigate the influence of high dose of dexamethasone on inflammatory mediators and apoptosis of rats with severe acute pancreatitis (SAP). METHODS: SAP rats were randomly assigned to the model group and treatment group while the normal rats were assigned to the sham operation group. The mortality,ascite volumes,ascites/body weight ratio and pancreas pathological changes of all rats were observed at 3,6 and 12 h after operation. Their contents of amylase and endotoxin in plasma and contents of tumor necrosis factor (TNF-α),phospholipase A2 (PLA2) and IL-6 in serum were also determined. The microarray sections of their pancreatic tissues were prepared,terminal transferase dUTP nick end labeling (TUNEL) staining was performed and apoptotic indexes were calculated. RESULTS: There was no marked difference between treatment group and model group in survival. The contents of amylase and endotoxin in plasma and contents of TNF-α,PLA2 and IL-6 in serum,ascite volumes,ascites/body weight ratio and pancreas pathological scores were all lower in treatment group than in model group to different extents at different time points P < 0.05,58.3 (26.4) ng/L vs 77.535 (42.157) ng/L in TNF-α content,8.00 (2.00) points vs 9.00 (2.00) points in pathological score of pancreas respectively; P < 0.01,0.042 (0.018) EU/mL vs 0.056 (0.0195) EU/mL in endotoxin content,7791 (1863) U/L vs 9195 (1298) U/L in plasma amylase content,1.53 (0.79) vs 2.38 (1.10) in ascites/body weight ratio,8.00 (1.00) points vs 11.00 (1.50) points in pathological score of pancreas; P < 0.001,3.36 (1.56) ng/L vs 5.65 (1.08) ng/L in IL-6 content,4.50 (2.00) vs 7.20 (2.00),4.20 (1.60) vs 6.40 (2.30),3.40 (2.70) vs 7.90 (1.70) in ascite volumes,respectively. The apoptotic indexes of pancreas head and pancreas tail were all higher in treatment group than in model group at 6 h P < 0.01,0.00 (2.00)% vs 0.00 (0.00)%,0.20 (1.80) vs 0.00 (0.00) in apoptosis indexes,respectively. CONCLUSION: The mechanism of dexamethasone treatment in acute pancreatitis is related to its inhibition of inflammatory mediator generation and induction of pancreatic acinar cell apoptosis. | Xi-Ping Zhang Li Chen Qi-Fang Hu Hua Tian Ru-Jun Xu Zhi-Wei Wang Ke-Yi Wang Qi-Hui Cheng Wei Yan Yun Li Qing-Yu Li Qing He Fei Wang | 2007 | World Journal of Gastroenterology2007，13，41： | 29 |
| 20 | Immunological synergistic mechanisms of trans-/cis-stilbene glycosides in Heshouwu-related idiosyncratic liver injury显示文摘Heshouwu is a traditional non-toxic Chinese medicine commonly used in the clinical setting;however,clinical cases of Heshouwu-induced hepatotoxicity have been frequently reported^([1]).Pharmacoepidemiological studies have found that Heshouwu-induced liver injury occurs only in a small fraction of individuals taking the drug;these patients presented with the typ- | Lanzhi He Ping Yin Yakun Meng Jinfa Tang Tingting He Ming Niu Yuming Guo Yun Zhu Jing Jing Chunyu Li Zhijie Ma Jiabo Wang Zhaofang Bai Xiaohe Xiao | 2017 | Science Bulletin2017，62，11： | 29 |
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