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| 1 | Grassland degradation in Northern Tibet based on remote sensing data显示文摘This study selected vegetation cover as the main evaluation index, calculated the grassland degradation index (GDI) and established the remote sensing monitoring and evaluation system for grassland degradation in Northern Tibet, according to the National Standard (GB19377-2003), based on the remote sensing data such as NDVI data derived from NOAA/AVHRR with a spatial resolution of 8 km of 1981-2000, from SPOT/VGT with a spatial resolution of 1 km of 2001 and from MODIS with a spatial resolution of 0.25 km of 2002-2004 respectively in this area, in combination with the actual condition of grassland degradation. The grassland degradation processes and their responses to climate change during 1981-2004 were discussed and analyzed in this paper. The result indicated that grassland degradation in Northern Tibet is very serious, and the mean value of GDI in recent 20 years is 2.54 which belongs to the serious degradation grade. From 1981 to 2004, the GDI fluctuated distinctly with great interannual variations in the proportion of degradation degree and GDI but the general tendency turned to severe-grade during this period with the grassland degradation grade changed from light degraded to serious degraded in Northern Tibet. The extremely serious degraded and serious degraded grassland occupied 1.7% and 8.0% of the study area, the moderate and light degraded grassland accounted for 13.2% and 27.9% respectively, and un-degraded grassland occupied 49.2% of the total grassland area in 2004. The grassland degradation was serious, especially in the conjunctive area of Naqu, Biru and Jiali counties, the headstream of the Yangtze River lying in the Galadandong snow mountain and glaciers, the area along the Qinghai-Tibet highway and railway, and areas around the Tanggula and Nianqingtanggula snow mountains and glaciers. So the snow mountains and glaciers as well as their adjacent areas in Northern Tibet were sensitive to climate change and the areas along the vital communication line with frequent human activities experienced relatively serious grassland degradation. | GAO Qingzhu LI Yu'e WAN Yunfan LIN Erda XIONG Wei JIANGCUN Wangzha WANG Baoshan LI Wenfu | 2006 | Journal of Geographical Sciences2006，16，2： | 10 |
| 2 | Process Optimization and Content Determination of Total Flavonoids of Zhuang Medicine Clerodendrum japonicum(Thumb.)Sweet显示文摘[Objectives]To optimize the extraction process of total flavonoids of Zhuang medicine Clerodendrum japonicum(Thumb.)Sweet and establish a method for its extraction and content determination.[Methods]The total flavonoids of C.japonicum were extracted by reflux extraction method.Through a single factor experiment,the effects of extraction method,extraction solvent concentration,extraction volume and extraction time on the total flavonoids content of medicinal materials were investigated to select the optimal extraction process of the total flavonoids of C.japonicum.[Results]The optimal extraction process of the total flavonoids of C.japonicum was 75%ethanol,1∶20 solid-to-liquid ratio,and 50 min reflux extraction time.[Conclusions]This method can effectively determine the content of total flavonoids in C.japonicum and provide a certain scientific basis for the study of the quality standard of this Zhuang medicine.This method has high reproducibility.It is stable and feasible in extraction of total flavonoids from Zhuang medicine C.japonicum. | Jiangcun WEI Zujie QIN Yunli TANG Nong TANG Liping QIN Yan MA Mingchan WEI Fengxian ZHAO | 2020 | Medicinal Plant2020，11，4： | 1 |
| 3 | Extraction Process and Content Determination of Caffeic Acid in Laggera alata from Different Production Areas of Guangxi显示文摘[Objectives] To establish a method for determining the content of Laggera alata( D. Don) Sch. Bip. Ex Oliv. using caffeic acid the target component,and to compare the content of caffeic acid in the medicinal materials of L. alata in different production areas of Guangxi.[Methods]The content was determined by Inertsil~ODS-3 chromatographic column C_(18)( 4. 60 mm × 250 mm,5 μm,mobile phase: acetonitrile-0. 1% phosphoric acid( 22∶ 78),detection wavelength: 320 nm,flow rate: 1. 0 m L/min,column temperature: 30℃,and injection volume: 10 μL. [Results] The caffeic acid showed a good linear relationship in the range of injection volume of 0. 025 92-0. 259 2 μg( R =0. 999 5). The average recovery rate was 98. 33%( RSD = 1. 85%). L. alata in different production areas of Guangxi contained the caffeic acid,and there was a great difference in the caffeic acid. L. alata in Baise had the highest content of caffeic acid,while that in Guilin had the lowest content of caffeic acid. [Conclusions]This method can accurately determine the content of caffeic acid and is expected provide a scientific basis for the development and utilization of herbal medicine L. alata. | Jiangcun WEI Yong CHEN Zujie QIN Jiabao MA Shengbin CHEN Zuliang QUE Jinzhou LI Zijun CHEN | 2018 | Medicinal Plant2018，9，4： | 1 |
| 4 | Processing of Rhubarb and Determination of Five Free Anthraquinones in Raw Rhubarb and Stewed Rhubarb显示文摘[Objectives]To determine the content of five kinds of free anthraquinones in raw rhubarb and stewed rhubarb by HPLC,and compare their content difference,so as to reveal the change rule of material basis before and after processing of traditional Chinese medicine.[Methods]The content was determined by HPLC with Thermo BDS HYPERSII C18 column(4.6 mm×250 mm,5μm)as chromatographic column and methanol-0.1%phosphoric acid(85∶15)aqueous solution as mobile phase;the detection wavelength was 256 nm,the flow rate was 1.0 mL/min,the column temperature was 30℃,and the injection volume was 10μL.[Results]Aloe-emodin,rhein,emodin,chrysophanol and emodin methyl ether showed a good linear relationship in the linear range of 0.0207-0.414(R2=0.9999),0.1721-3.442(R2=0.9996),0.0203-0.406(R2=0.9999),0.148-2.96(R2=0.9998)and 0.164-3.28(R2=0.9993),respectively;the content of aloe-emodin decreased by about 8.26%,and rhein decreased by about 9.21%;the content of emodin increased by about 16.44%,chrysophanol increased by about 16.12%,and emodin methyl ether increased by about 20.01%,indicating that the processing technology had different effects on the components of rhubarb.[Conclusions]The content of five free anthraquinones in rhubarb was affected to different degrees after processing,and this method could be used as a quality control method for rhubarb and its different processed products. | Huazhen SU Xiumei MA Jiangcun WEI Yong CHEN Shaoyuan HUANG Liqing LIANG Meiyan QIU Fengxian ZHAO Qian HAN | 2020 | Medicinal Plant2020，11，5： | 1 |
| 5 | Comparative Study on Effects of Rheum officinale and Wine Prepared Rheum palmatum Dachengqi Decoction on Laxative Effect of Mice显示文摘[Objectives] To study the differences in the effects of Rheum officinale and wine prepared Rheum palmatum Dachengqi Decoction(DCQ) on laxative effect of mice.[Methods]First,72 mice were randomly divided into positive control group,blank control group,6 and10 g/kg dose R.officinale DCQ groups,6 and 10 g/kg dose wine prepared R.palmatum DCQ groups.After administration at the dose of20 m L/kg,metabolic cage method and carbon powder propulsion were used to conduct Na+-K+-ATPasc activity experiment,and the comparison was made for the differences in the normal laxative effect of R.officinale and wine prepared R.palmatum on DCQ,the carbon powder propulsion rate,and Na+-K+-ATPasc activit.[Results]There are differences in the laxative effect of R.officinale and wine prepared R.palmatum on DCQ,but their differences in changes are,to some extent,connected with R.officinale and wine prepared R.palmatum.From comparison of R.officinale DCQ group,wine prepared R.palmatum DCQ group,blank control group,and positive control group,it is found that there are differences in the normal laxative effect of normal mice,carbon powder propulsion,and inhibition of Na+-K+-ATPase activity.In addition,the comparison of drug administration groups indicates that the differences in the effects of R.officinale DCQ group are most significant,and there are significant differences in the 6 and 10 g/kg groups.[Conclusions]There are certain differences in the influence of R.officinale and wine prepared R.palmatum Dachengqi Decoction on normal laxative effect of mice,which may be connected with the fact that changes in main chemical components of king drugs R.officinale and wine prepared R.palmatum lead to changes in the dissolution amount of anthraquinones in the compound Dachengqi Decoction. | Jiangcun WEI Yong CHEN Zhen XIE Dongmei HUANG Zijun CHEN Jinzhou LI Zuliang QUE Qian HAN | 2018 | Medicinal Plant2018，9，6： | 1 |
| 6 | Effects of Leaf Extract of Phyllanthus reticulatus from Guangxi on Human Gastric Cancer SGC-7901 Cells in vitro显示文摘[Objectives]To observe the effect of drug-containing serum from different extracts of Phyllanthus reticulatus leaf on the proliferation of human gastric cancer SGC-7901 cells and the effect of in vivo on the life extension rate of mice with H22 ascites tumor,and to investigate the effects of chemical components such as Corilagin,ethyl gallate,ethyl brevifolincarboxylate,and gallic acid on the proliferation of human gastric cancer SGC-7901 cells.[Methods]MTT method was used to observe the inhibitory effect of drug-containing serum of different extracts of P.reticulatus leaf on human gastric cancer SGC-7901 cells in vitro to determine its active site.The active site was used as the research object to establish a Kunming mouse ascites tumor model,to investigate the effect on the life extension rate of mice with H22 ascites tumor,to further separate the monomer components from the effective fraction and investigate its effect on human gastric cancer SGC-7901 cells.[Results]Compared with the 10%blank serum control group,10%drug-containing serum of ethyl acetate and n-butanol in P.reticulatus leaf had significant inhibitory effect on the proliferation of human gastric cancer SGC-7901 cells,and the inhibition rate was 34.99%and 28.68%,respectively(P<0.05).Compared with the blank group,the survival time of the high dose group of ethyl acetate in P.reticulatus leaf was significant(P<0.05).Gallic acid,Corilagin,and Brevifolincarboxylic acid ethyl ester had a significant inhibitory effect on the proliferation of human gastric cancer SGC-7901 cells,with IC 50 of 26.52,70.45,and 158.86μg/mL,respectively.Ethyl gallate had no inhibitory effect on the proliferation of human gastric cancer SGC-7901 cells,and its IC 50 was 251.96μg/mL.[Conclusions]The drug-containing serum of ethyl acetate and n-butanol extract of P.reticulatus can inhibit the proliferation of human gastric cancer SGC-7901 cells.Ethyl acetate of P.reticulatus leaves can increase the life extension rate of mice with H22 ascites tumor.Gallic acid,Corilagin,and Brevifolincarboxylic acid ethyl ester isolated from its active site are the material basis for inhibiting the proliferation of human gastric cancer SGC-7901 cells. | Yunli TANG Jiangcun WEI Huaien WU Chenyan LIANG Zuowen ZHENG Qiuheng RUAN Yuanzhi WU | 2019 | Medicinal Plant2019，10，5： | 1 |
| 7 | A Preliminary Study on Effects of Zhuang Medicine Clerodendrum japonicum(Thunb.)Sweet on Acute Tonsillitis显示文摘[Objectives]To study the effect of Zhuang medicine Clerodendrum japonicum(Thunb.)Sweet on acute tonsillitis.[Methods]The antibacterial,antipyretic,anti-inflammatory and analgesic effects of the extract of Zhuang medicine C.japonicum(Thunb.)Sweet were observed.[Results]Zhuang medicine C.japonicum(Thunb.)Sweet showed a good inhibitory effect onα-hemolytic streptococcus,β-hemolytic streptococcs,Staphylococcus citreus,Staphylococcus epidermidis,Staphylococcus aureus,Pseudomonas aeruginosa,Proteus and Escherichia coli;it showed a good inhibitory effect on the infection of Staphylococcus aureus andβ-hemolytic streptococcs in vivo;it could significantly reduce the elevated body temperature of rabbits injected with the triple vaccine and significantly increase the pain threshold of thermally stimulated mice.[Conclusions]Zhuang Medicine C.japonicum(Thunb.)Sweet has positive significance in the treatment of acute tonsillitis instead of antibiotics. | Jiangcun WEI Xiumei MA Bing QING Xuan GUAN Liping QIN Li LONG Hong LEI Zujie QIN | 2023 | Medicinal Plant2023，14，2： | 0 |
| 8 | TLC Identification of Yao Medicine Pileostegia tomentellal and Extraction Technology and Content Determination of Umbelliferone显示文摘[Objectives]To establish a TLC and content determination method of Pileostegia tomentellal,with umbelliferone as the indicator component.[Methods]TLC identification was performed by silica gel G thin layer plate with n-hexane-ethyl acetate(4:3)as the developing agent,and the plate was examined by UV lamp(365 nm).The umbelliferone content was determined by HPLC:Inertsil ODS-3 C 18 column(4.60 mm×250 mm,5μm);mobile phase acetonitrile-0.2%phosphoric acid gradient elution;detection wavelength 320 nm,flow rate 1.0 mL/min,column temperature 30℃,injection volume 10μL.[Results]The chromatogram of P.tomentellal showed the same color spot in the same position as that of reference medicinal material,and the spot was clear with good specificity.Umbelliferone showed a good linear relationship when the injection volume was 2.63-131.27μg/mL(R^(2)=0.9997).The average recovery of umbelliferone in the low,middle and high adding groups of P.tomentellal was 99.57%and the RSD was 2.15%.[Conclusions]The method can effectively identify Yao medicine P.tomentellal and accurately determine the content of umbelliferone in medicinal materials,which will provide a scientific basis for the development and utilization of medicinal resources of Yao medicine P.tomentellal. | Jiangcun WEI Xiumei MA Meiyan QIU Bing QING Jingrong LU Hong LEI Xiaodong HUANG Wen ZHONG | 2024 | Medicinal Plant2024，15，2： | 0 |
| 9 | Research Progress in the Application of Network Pharmacology in Traditional Chinese Medicine and Compound Prescriptions显示文摘Network pharmacology is a new discipline based on the theory of systems biology,mainly for network analysis of biological systems.It selects specific signal nodes for multi-target drug molecular design.This article summarizes the application of network pharmacology in traditional Chinese medicine and compound prescriptions in recent years from the research progress of network pharmacology,the current research status of single herbs,and the research and application of compound prescriptions. | Jiangcun WEI Zujie QIN Xiumei MA Wen ZHONG Zongxi SUN Liping QIN Yinghong HUANG Xianyi SHI | 2021 | Medicinal Plant2021，12，3： | 0 |
| 10 | Potential Preventive and Therapeutic Effects of Traditional Chinese Medicine Compound Prescription and Traditional Chinese Medicine Products on COVID-19显示文摘This paper mainly analyzes the traditional Chinese medicine compound prescription and traditional Chinese medicine products with remarkable epidemic prevention effect since the outbreak of COVID-19,and discusses their potential prevention and treatment effects,so as to provide a basis for the further development and application of traditional Chinese medicine compound prescription and traditional Chinese medicine products against COVID-19. | Wen ZHONG Haisheng ZENG Xiumei MA Jiangcun WEI Dongmei HUANG Chengtong LIU Mengxia YANG | 2021 | Medicinal Plant2021，12，3： | 0 |
| 11 | Typical HPLC Chromatogram of Petroleum Ether in Abutilon indicum(L.) Sweet from Different Habitats显示文摘[Objectives] To study the typical HPLC chromatogram of petroleum ether in Abutilon indicum (L.) Sweet from different habitats, in order to provide experimental basis for the quality control of A. indicum and the spectrum-efficacy relationship of petroleum ether.[Methods] The HPLC chromatogram of petroleum ether was obtained using Agilent SB-C 18 (4.6 mm×250 mm, 5 μm) and acetine-0.1% phosphoric acid solution for gradient elution at temperature of 30 ℃, flow rate of 1.0 mL/min and detection wavelength of 205 nm.[Results] The typical HPLC chromatogram of petroleum ether in A. indicum was preliminarily established, with 12 characteristic peaks. It was verified by A. indicum from different habitats, characterized by reproducibility and stability.[Conclusions] Typical HPLC chromatogram provides a stable new method for the quality control of petroleum ether in A. indicum . This study provides experimental basis for further study on the medicinal parts of A. indicum . | Yong CHEN Jiabao MA Jiangcun WEI Qian HAN | 2019 | Medicinal Plant2019，10，4： | 0 |
| 12 | Anti-tumor Effect of Paclitaxel Enhanced by Psoralen at the Cellular Level显示文摘[Objectives]To explore the effect of psoralen combined with paclitaxel on the apoptosis of MCF-7 cells.[Methods]The effects of different concentrations of psoralen,paclitaxel,or the combination of psoralen and paclitaxel on cell viability were detected using CCK-8 assay kit.Cell cycle distribution and apoptosis after 24 h of psoralen(0.16,0.32,0.64 mmol/L),paclitaxel(0.1μmol/L),combined action of psoralen(0.32 mmol/L)and paclitaxel(0.1μmol/L)were detected using flow cytometry.[Results]Lower concentration of psoralen(0.04-0.32 mmol/L)showed no significant inhibitory effect on cells.After combined with paclitaxel,the inhibitory effect on MCF-7 cell proliferation was significantly higher than that of the group treated alone.Compared with the paclitaxel group,the cell apoptosis rate in the drug combination group was significantly increased.Different low concentrations of psoralen can block the cell cycle of MCF-7 at G 0/G 1 phase,while paclitaxel can block the cell cycle at G 2/M phase.After combined action,the number of cells blocked at G 2/M phase decreased.[Conclusions]Overall,the combined effect of psoralen and paclitaxel can enhance anti-tumor ability by inhibiting cell proliferation,inducing apoptosis,and blocking cell cycle. | Yinghong HUANG Linqian CHEN Yaping WU Xian PENG Xuemei FANG Chunye LU Jiangcun WEI | 2023 | Medicinal Plant2023，14，6： | 0 |
| 13 | Preliminary Study on Quality Analysis of Yao Medicine Pileostegia tomentella Hand.-Mazz.显示文摘[Objectives]To establish a method for quality analysis of Medicine Pileostegia tomentella Hand.-Mazz.[Methods]According to the testing method of Chinese Pharmacopoeia(2020 Edition),the water content,total ash and alcohol-soluble extract of P.tomentella Hand.-Mazz.were determined.[Results]10 batches of P.tomentella Hand.-Mazz.from different habitats and in different collection time in Guangxi all had the same plant morphology and medicinal properties.The experimental results showed that the water content was 7.92%-10.35%,the total ash content was 5.53%-6.62%,the acid-insoluble ash content was 0.59%-0.76%,and the extract content was 11.63%-16.12%.[Conclusions]The identification method in this study can be used for the quality analysis of P.tomentella Hand.-Mazz.,and the experimental results can provide a scientific basis for the development and utilization of P.tomentella Hand.-Mazz.and the establishment of its quality standard. | Xiaodong HUANG Xiumei MA Yong TAN Jiangcun WEI Wen ZHONG Bing QING Qingmei DENG Hong LEI | 2023 | Medicinal Plant2023，14，6： | 0 |
| 14 | Clinical Observation and Safety Evaluation of Zhuang Medicine Xiaoyan Zhiyang Formula for Dermatosis显示文摘[Objectives]To observe the clinical efficacy and safety of Zhuang Medicine Xiaoyan Zhiyang Formula by retrospective analysis of clinical use.[Methods]A total of 225 cases were collected,including 117 males and 108 females,115 cases of contact dermatitis and 110 cases of acute eczema.Zhuang Medicine Xiaoyan Zhiyang Formula was administered to 225 patients with contact dermatitis and acute eczema and the clinical efficacy was evaluated.[Results]After one week of treatment,among 225 patients with contact dermatitis and acute eczema,76 cases were cured(33.78%),133 cases showed marked response(59.11%),16 cases(7.11%)were improved,0 case was ineffective,and the overall response rate was 92.89%.After one week of Zhuang Medicine Xiaoyan Zhiyang Formula treatment,the symptoms of itching,pain and swelling were significantly improved in all patients,and the rash was partially crusted without new symptom,and there were no complications and adverse reactions.[Conclusions]Zhuang Medicine Xiaoyan Zhiyang Formula therapy has a remarkable effect in the treatment of contact dermatitis and acute eczema. | Wen ZHONG Xixi QI Jiangcun WEI Xian PENG Fangmei LI | 2023 | Medicinal Plant2023，14，6： | 0 |
| 15 | Research Overview of Zhuang Medicine Fumigation Lotions显示文摘At present,there is little research on the application of Zhuang medicine fumigation lotions in skin diseases,and there is no research on related preparation processes and quality standards.In this paper,the characteristics of its formulation and the research progress in skin diseases are reviewed,in order to deepen the understanding of Zhuang medicine fumigation lotions and provide a more detailed theoretical basis for its clinical research,development and application. | Bing QING Jiangcun WEI Fangmei LI Xianyi SHI Li LONG Xian PENG Meiyan QIU Wen ZHONG Zujie QIN | 2024 | Medicinal Plant2024，15，1： | 0 |
| 16 | Effect of Radix Ilicis Pubescentis on the Rats with Hyperlipidemia显示文摘[Objectives]To study the hypolipidemic effect of Radix Ilicis Pubescentis and to provide experimental scientific basis for the study of hypolipidemic mechanism of Radix Ilicis Pubescentis.[Methods]60 healthy(SD)male rats were selected,10 rats were given basic feed and pure water as blank control group,and the other 50 rats were fed with high-fat diet for 4 weeks.After establishing the hyperlipidemic rat model,the rats were randomly divided into model group,high,middle and low dose groups(1000,800,500 mg/kg),and positive control group(simvastatin 0.4 mg/mL).The corresponding drugs were intragastrically administered to the rats in each group(blank control group and model group intragastrically administered with normal saline).Four weeks later,blood samples from abdominal aorta were taken to detect serum total cholesterol(TC),serum triglyceride(TG),low density lipoprotein cholesterol(LDL-C),and high density lipoprotein cholesterol(HDL-C).[Results]Compared with the blank control group,TC,TG and LDL-C increased while HDL-C decreased in the model group(P<0.05);compared with the model group,TC,TG and LDL-C in Radix Ilicis Pubescentis group decreased to varying degrees,and the content of HDL-C in serum of rats had no statistical significance.Compared with the blank control group,the serum TC and TG contents of the model group,positive control group,high,middle and low dose groups of Radix Ilicis Pubescentis extract significantly increased(P<0.01 or 0.05),and the serum LDL-C content of rats decreased(P<0.05).[Conclusions]The extract of Radix Ilicis Pubescentis had a certain lipid-lowering effect on hyperlipidemia in rats,and had obvious preventive and therapeutic effect on hyperlipidemia. | Chaoyan QIN Jiangcun WEI Peitao XIE Wen ZHONG Shengbin CHEN Fengxian ZHAO Qian HAN | 2020 | Medicinal Plant2020，11，4： | 0 |
| 17 | Thin Layer Identification of Jiedu Shengxue Granules and Determination of Notoginsenoside R1 Content显示文摘[Objectives]To establish the quality standard of hospital preparation Jiedu Shengxue granules.[Methods]Scleromitrion diffusum and Prunella vulgaris in Jiedu Shengxue granules were qualitatively identified by thin layer chromatography(TLC).A high performance liquid chromatography(HPLC)was established to determine the content of notoginsenoside R1 in the granule.[Results]The traditional Chinese medicinal materials in Jiedu Shengxue granules could be identified by TLC,and the characteristic spots were stable and clear.Notoginsenoside R1 had a good linear relationship in the range of 10.45-104.5μg/mL,with an average recovery of 98.52%and RSD=2.36%.[Conclusions]TLC and HPLC,as the quality control methods of Jiedu Shengxue granules,have high accuracy and good repeatability,which lays a foundation for the quality control of this mixture. | Zhenying FU Bing QING Yinghong HUANG Xianyi SHI Meiyan QIU Xian PENG Jiangcun WEI Fengzhen LI Wen ZHONG | 2024 | Medicinal Plant2024，15，1： | 0 |
| 18 | Study on the Chemical Constituents of Clerodendrum japonicum,a Zhuang Medicine显示文摘[Objectives]To make a preliminary study on the chemical constituents of Clerodendrum japonicum,a Zhuang medicine,so as to provide theoretical support and scientific basis for exploring its medicinal value and comprehensive development and utilization.[Methods]The compounds were separated by macroporous adsorption resin,silica gel,Sephadex LH-20,ODS chromatographic column and preparative high performance liquid chromatography,and their structures were identified by NMR and MS data.[Results]8 compounds were isolated and identified from Clerodendrum japonicum extract with 80%ethanol solution:(1)fumaric acid,(2)trans-p-hydroxycinnamic acid,(3)cis-p-hydroxycinnamic acid,(4)ethyl caffeate,(5)acteoside,(6)kaempferol,(7)apigenin-7-O-β-D-glucopyranoside,(8)caffeic acid.[Conclusions]8 compounds were isolated and purified from the ethanol extract of Clerodendrum japonicum. | Jiangcun WEI Zujie QIN Wenwei CAI Haisheng ZENG Liping QIN Xinying MO Yuping TAN Guilin YANG | 2021 | Medicinal Plant2021，12，6： | 0 |
| 19 | Analysis on Quality Control of Argyreia acuta Lour显示文摘[Objectives]To establish a method for caffeic acid content determination and thin-layer chromatography identification of the Zhuang medicine Argyreia acuta Lour.[Methods]Silica gel GF254(thin-layer plate),toluene-formic acid-ethyl acetate(V∶V∶V=5∶1.2∶3)(developing agent)and 365 nm ultraviolet light were used for thin-layer chromatography identification.Under the chromatographic conditions of column of Thermo SCIENTIFIC Hypersil C18(5μm,4.60 mm×250 mm),mobile phase of methanol-0.1%phosphoric acid(23∶77),detection wavelength of 320 nm,column temperature of 30℃,sample size of 10μL and flow rate of 1.0 mL/min,the content of caffeic acid in A.acuta Lour was determined.[Results]Caffeic acid can be detected in thin layer chromatography,with strong specificity and clear spots.When the sample size of caffeic acid is within the range of 0.0151-0.4530μg(R^(2)=0.9999),the content of caffeic acid showed a relatively good linear relationship with the peak area.The average recovery rate of A.acuta Lour was 99.94%(RSD=2.68%),97.56%(RSD=1.57%)and 99.79%(RSD=2.05%),respectively.[Conclusions]This method can effectively identify A.acuta Lour,and can accurately determine the content of caffeic acid in A.acuta Lour.It has characteristics of high accuracy,high precision,good color rendering stability,good reproducibility and fast analysis speed. | Ru ZHANG Jiangcun WEI Chunli TANG Bihua NONG Guangli LU Yanxiu WANG | 2020 | Medicinal Plant2020，11，6： | 0 |
| 20 | Extraction Process and Content Determination of Total Flavonoids in Lonicera japonica显示文摘[Objectives]To optimize the extraction process of total flavonoids of Lonicerae japonica and establish a method for its extraction and content determination.[Methods]The total flavonoids of L.japonica were extracted by reflux extraction method.Through a single factor experiment,the effects of extraction method,extraction solvent concentration,extraction volume and extraction time on the total flavonoids content of medicinal materials were investigated to select the optimal extraction process of the total flavonoids of L.japonica.[Results]The optimal extraction process of the total flavonoids of L.japonica was 70%ethanol,1∶30 of solid-to-liquid ratio,and 1.0 h of reflux extraction time.[Conclusions]This method can effectively determine the content of total flavonoids in L.japonica and is expected to provide a certain scientific basis for the study of the quality standard of L.japonica.This method has high reproducibility.It is stable and feasible in extraction of total flavonoids from L.japonica. | Yao HUANG Xiumei MA Chao ZENG Xiaoling CAI Jie HE Xi FENG Jiangcun WEI Hongxia CHEN | 2020 | Medicinal Plant2020，11，6： | 0 |
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