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| 1 | Mesenchymal stem cell treatment improves outcome of COVID-19 patients via multiple immunomodulatory mechanisms显示文摘The infusion of coronavirus disease 2019(COVID-19)patients with mesenchymal stem cells(MSCs)potentially improves clinical symptoms,but the underlying mechanism remains unclear.We conducted a randomized,single-blind,placebo-controlled(29 patients/group)phase II clinical trial to validate previous findings and explore the potential mechanisms.Patients treated with umbilical cord-derived MSCs exhibited a shorter hospital stay(P=0.0198)and less time required for symptoms remission(P=0.0194)than those who received placebo.Based on chest images,both severe and critical patients treated with MSCs showed improvement by day 7(P=0.0099)and day 21(P=0.0084).MSC-treated patients had fewer adverse events.MSC infusion reduced the levels of C-reactive protein,proinflammatory cytokines,and neutrophil extracellular traps(NETs)and promoted the maintenance of SARS-CoV-2-specific antibodies.To explore how MSCs modulate the immune system,we employed single-cell RNA sequencing analysis on peripheral blood.Our analysis identified a novel subpopulation of VNN2+hematopoietic stem/progenitorlike(HSPC-like)cells expressing CSF3R and PTPRE that were mobilized following MSC infusion.Genes encoding chemotaxis factors—CX3CR1 and L-selectin—were upregulated in various immune cells.MSC treatment also regulated B cell subsets and increased the expression of costimulatory CD28 in T cells in vivo and in vitro.In addition,an in vivo mouse study confirmed that MSCs suppressed NET release and reduced venous thrombosis by upregulating kindlin-3 signaling.Together,our results underscore the role of MSCs in improving COVID-19 patient outcomes via maintenance of immune homeostasis. | Rongjia Zhu Tingdong Yan Yingmei Feng Yan Liu Hongcui Cao Gongxin Peng Yanlei Yang Zhen Xu Jingqi Liu Wei Hou Xiaoyue Wang Zhe Li Luchan Deng Shihua Wang Jing Li Qin Han Hongling Li Guangliang Shan Yinghao Cao Xingyan An Jianshe Yan Zhonghui Zhang Huafei Li Xuebin Qu Jiaqi Zhu Shumin Zhou Jiao Wang Fengchun Zhang Jinming Gao Ronghua Jin Dayong Xu Yan-Qing Ma Tao Huang Shuang Peng Zhi Zheng Ilia Stambler Eric Gilson Lee Wei Lim Alexey Moskalev Antonio Cano Sasanka Chakrabarti Brun Ulfhake Huanxing Su Haoying Xu Sihuan Xu Feng Wei Holly MBrown-Borg Kyung-Jin Min Georgina Ellison-Hughes Calogero Caruso Kunlin Jin Robert Chunhua Zhao | 2021 | Cell Research2021，31，12： | 5 |
| 2 | Propofol attenuates high glucose-induced P66shc expression in human umbilical vein endothelial cells through Sirt1显示文摘Perioperative hyperglycemia is a common metabolic disorder in clinic settings.Hyperglycemia leads to endothelial inflammation,endothelial cell apoptosis,and dysfunction,thus resulting in endothelial injury.Propofol(2,6-diisopropylphenol)is a widely used intravenous anesthetic in clinic settings.Our previous study indicated that propofol inhibits mitochondrial reactive oxygen species(ROS)production via down-regulation of phosphatase A2(PP2A)expression,inhibition of Ser36-p66shc dephosphorylation and mitochondrial translocation,thus improving high glucose-induced endothelial injury.The expression of p66shc was inhibited by propofol in hyperglycemic human umbilical vein endothelial cells(HUVECs).However,the mechanism by which propofol inhibits p66shc expression in hyperglycemic HUVECs is still obscure.In the present study,we mainly examined how propofol inhibited high glucose-induced p66shc expression in HUVECs.Compared with 5 mM glucose treatment,high glucose increased p66shc expression and decreased sirt1 expression,which was inhibited by propofol treatment.Moreover,EX527(a sirt1 inhibitor)reversed the effect of propofol against high glucose-induced p66shc expression.However,EX527 did not reverse the effects of propofol against high glucose-induced ROS accumulation,endothelial inflammation,and apoptosis.Furthermore,when cells were incubated with propofol,EX527,and FTY720(a PP2A activator)simultaneously,the effects of propofol against high glucose-induced ROS accumulation,inflammation,and apoptosis were reversed.Our results suggested that propofol inhibited high glucose-induced p66shc expression via upregulation of sirt1 expression in hyperglycemic HUVECs.Moreover,propofol protects against high glucose-mediated ROS accumulation and endothelial injury via both inhibition of p66shc expression and dephosphorylation of Ser36-p66shc. | Jing Wang Jie Qi Qichao Wu Hui Jiang Yuehao Yin Yan Huanx Yanjun Zhao Minmin Zhu | 2019 | Acta Biochimica et Biophysica Sinica2019，51，2： | 5 |
| 3 | Identification of IFN-γ-producing innate B cells显示文摘 | Yan Bao Xingguang Liu Chaofeng Han Sheng Xu Bin Xie Qian Zhang Yan Gu Jin Hou Li Qian Cheng Qian Huanxing Han Xuetao Cao | 2014 | Cell Research2014，24，2： | 3 |
| 4 | Contrasting neuropathology and functional recovery after spinal cord injury in developing and adult rats显示文摘Conflicting findings exist regarding the link between functional recovery and the regrowth of spinal tracts across the lesion leading to the restoration of functional contacts. In the present study, we investigated whether functional locomotor recovery was attributable to anatomical regeneration at postnatal day 1 (PN1), PN7, PN14 and in adult rats two months after transection injury at the tenth thoracic segment of the spinal cord. The Basso, Beattie, and Bresnahan scores showed that transection led to a failure of hindlimb locomotor function in PN14 and adult rats. However, PN1 and PN7 rats showed a significant level of stepping function after complete spinal cord transection. Unexpectedly, unlike the transected PN14 and adult rats in which the spinal cord underwent limited secondary degeneration and showed a scar at the lesion site, the rats transected at PN1 and PN7 showed massive secondary degeneration both anterograde and retrograde, leaving a >5-mm gap between the two stumps. Furthermore, retrograde tracing with fluorogold (FG) also showed that FG did not cross the transection site in PN1 and PN7 rats as in PN14 and adult rats, and re-transection of the cord caused no apparent loss in locomotor performance in the rats transected at PN1. Thus, these three lines of evidence strongly indicated that the functional recovery after transection in neonatal rats is independent of regrowth of spinal tracts across the lesion site. Our results support the notion that the recovery of locomotor function in developing rats may be due to intrinsic adaptations in the spinal circuitry below the lesion that control hindlimb locomotor activity rather than the regrowth of spinal tracts across the lesion. The difference in secondary degeneration between neonatal and adult rats remains to be explored. | Qiuju Yuan Huanxing Su Kin Chiu Wutian Wu Zhi-Xiu Lin | 2013 | Neuroscience Bulletin2013，29，4： | 2 |
| 5 | Using induced pluripotent stem cells for modeling Parkinson’s disease显示文摘Parkinson’s disease(PD)is an age-related neurodegenerative disease caused by the progressive loss of dopaminergic(DA)neurons in the substantia nigra.As DA neurons degenerate,PD patients gradually lose their ability of movement.To date no effective therapies are available for the treatment of PD and its pathogenesis remains unknown.Experimental models that appropriately mimic the development of PD are certainly needed for gaining mechanistic insights into PD pathogenesis and identifying new therapeutic targets.Human induced pluripotent stem cells(iPSCs)could provide a promising model for fundamental research and drug screening.In this review,we summarize various iPSCs-based PD models either derived from PD patients through reprogramming technology or established by gene-editing technology,and the promising application of iPSC-based PD models for mechanistic studies and drug testing. | Minjing Ke Cheong-Meng Chong Huanxing Su | 2019 | World Journal of Stem Cells2019，11，9： | 2 |
| 6 | Immediate expression of Cdh2 is essential for efficient neural differentiation of mouse induced pluripotent stem cells显示文摘 | Huanxing Su Lihui Wang Wenhao Huang Dajiang Qin Jinglei Cai Xiaoli Yao Chengqian Feng Zhiyuan Li Yitao Wang Kwok-Fai So Guangjin Pan Wutian Wu Duanqing Pei | 2013 | Stem Cell Research2013，，： | 1 |
| 7 | Investigation of the effects of humic acid and H_(2)O_(2) on the photocatalytic degradation of atrazine assisted by microwave显示文摘A solution of atrazine in a TiO_(2)suspension,an endocrine disruptor in natural water,was tentatively treated by microwave-assisted photocatalytic technique.The effects of mannitol,oxygen,humic acid,and hydrogen dioxide on the photodegradation rate were explored.The results could be deduced as follows:the photocatalytic degradation of atrazine fits the pseudo-first-order kinetic well with k=0.0328 s–1,and·OH was identified as the dominant reactant.Photodegradation of atrazine was hindered in the presence of humic acid,and the retardation effect increased as the concentration of humic acid increased.H_(2)O_(2)displayed a significant negative influence on atrazine photocatalysis efficiency.Based on intermediates identified with gas chromatography-mass spectrometry(GC-MS)and Liquid chromatography-mass spectrometry(LC-MS/MS)techniques,the main degradation routes of atrazine are proposed. | Chao QIN Shaogui YANG Cheng SUN Manjun ZHAN Rongjun WANG Huanxing CAI Jia ZHOU | 2010 | Frontiers of Environmental Science & Engineering2010，4，3： | 1 |
| 8 | Upregulation of my- eloid cell leukemia - 1 potentially modulates beclin - 1 - dependent autophagy in ischemic stroke in rats显示文摘 | XINGYONG C XICUI S HUANXING S | 2013 | BMC Neurosci2013，，1： | 1 |
| 9 | Studies on gas sensitive materials of γ-Fe2O3 doped with rare earth oxides显示文摘 | Xu Jiaqiang Zeng Huanxing Shen Yusheng | 1992 | J Rare Earth1992，19，2： | 1 |
| 10 | Upregulation of myeloid cell leukemia-1 potentially modulates beclin-1-dependent autophagy in is- chemic stroke in rats显示文摘 | Xingyong C Xicui S Huanxing S | 2013 | BMC Neurosci2013，14，1： | 1 |
| 11 | Studies on gas sensitive materials of γ-Fe2O3 doped with rare earth oxides显示文摘 | Xu Jiaqiang Zeng Huanxing Shen Yusheng | 1992 | J Rare Earth1992，19，2： | 1 |
| 12 | Respiratory toxicity of carbon nanotube: how worried should we be? 显示文摘 | MULLER J HUANX F LISON D | 2006 | Carbon2006，44，6： | 1 |
| 13 | Transplanted motoneurons derived from human induced pluripotent stem cells form functional connections with target muscle显示文摘 | Huanxing Su Lihui Wang Jinglei Cai Qiuju Yuan Xiaoying Yang Xiaoli Yao Wai-Man Wong Wenhao Huang Zhiyuan Li Jian-Bo Wan Yitao Wang Duanqing Pei Kwok-Fai So Dajiang Qin Wutian Wu | 2013 | Stem Cell Research2013，，1： | 1 |
| 14 | Generation of Human Induced Pluripotent Stem Cells from Umbilical Cord Matrix and Amniotic Membrane Mesenchymal Cells显示文摘 | Jinglei Cai Wen Li Huanxing Su | | 0，，15： | 1 |
| 15 | Reprogramming somatic cells to cells with neuronal characteristics by defined medium both in vitro and in vivo显示文摘Background:Currently,direct conversion from somatic cells to neurons requires virus-mediated delivery of at least one transcriptional factor or a combination of several small-molecule compounds.Delivery of transcriptional factors may affect genome stability,while small-molecule compounds may require more evaluations when applied in vivo.Thus,a defined medium with only conventional growth factors or additives for cell culture is desirable for inducing neuronal trans-differentiation.Results:Here,we report that a defined medium(5C)consisting of basic fibroblast growth factor(bFGF),N2 supplement,leukemia inhibitory factor,vitamin C(Vc),andβ-mercaptoethanol(βMe)induces the direct conversion of somatic cells to cells with neuronal characteristics.Application of 5C medium converted mouse embryonic fibroblasts(MEFs)into TuJ+neuronal-like cells,which were capable of survival after being transplanted into the mouse brain.The same 5C medium could convert primary rat astrocytes into neuronal-like cells with mature electrophysiology characteristics in vitro and facilitated the recovery of brain injury,possibly by inducing similar conversions,when infused into the mouse brain in vivo.Crucially,5C medium could also induce neuronal characteristics in several human cell types.Conclusions:In summary,this 5C medium not only provides a means to derive cells with neuronal characteristics without viral transfection in vitro but might also be useful to produce neurons in vivo for neurodegenerative disease treatment. | Songwei He Yiping Guo Yixin Zhang Yuan Li Chengqian Feng Xiang Li Lilong Lin Lin Guo Haitao Wang Chunhua Liu Yi Zheng Chuanming Luo Qiang Liu Fuhui Wang Hao Sun Lining Liang Lingyu Li Huanxing Su Jiekai Chen Duanqing Pei Hui Zheng | 2015 | Cell Regeneration2015，4，1： | 0 |
| 16 | A review and evaluation of nonroad diesel mobile machinery emission control in China显示文摘China’s emission control for nonroad diesel mobile machinery(NDMM)must deal with a fast increase in stock as well as regulations that are two decades behind those for on-road vehicles.This study provides the first large-scale review and evaluation of China’s NDMM policies,along with emission measurements and an investigation on diesel fuel quality.The sulfur contents of the investigated diesel declined from 430 ppm(median value)in 2011 to6-8 ppm during the 2017-2018 period.The emission control of NO_(x)and PM greatly improved with the shift from the China II to China IV standards,as demonstrated by engine tests and field NO_(x)measurements.However,the NO_(x)emission factors for non-type-approved engines were approximately twice the limits of the China II standards.Emission compliance based on bench tests was not sufficient to control actual emissions because the field-measured NO_(x)emission factors of all machinery ranged from 24%to 225%greater than the respective emission limits for the engines.These circumstances adversely affected the effectiveness of the regulations and policies for China’s emission control of NDMM.Nevertheless,the policies on new and in-use NDMM,as well as diesel fuel quality,prevented NO_(x)and PM emissions amounting to 4.4 Tg and 297.8 Gg during the period 2008-2017,respectively.The emission management strategy contributed to enhancing the international competitiveness of China’s NDMM industries by promoting advanced technologies.For effective NDMM emission control in the future,portable testing and noncontact remote supervision should be strengthened;also,the issue of noncompliant diesel should be addressed through rigorous control measures and financial penalties. | Pengju Bie Liang Ji Huanxing Cui Gang Li Shunli Liu Ying Yuan Kebin He Huan Liu | 2023 | Journal of Environmental Sciences2023，，1： | 0 |
| 17 | Multi-omics analysis reveals the evolutionary origin of diterpenoid alkaloid biosynthesis pathways in Aconitum显示文摘Diterpenoid alkaloids(DAs) have been often utilized in clinical practice due to their analgesic and anti-infammatory properties. Natural DAs are prevalent in the family Ranunculaceae, notably in the Aconitum genus. Nevertheless, the evolutionary origin of the biosynthesis pathway responsible for DA production remains unknown.In this study, we successfully assembled a highquality, pseudochromosome-level genome of the DA-rich species Aconitum vilmorinianum(A.vilmorinianum)(5.76 Gb). An A. vilmorinianumspecific whole-genome duplication event was discovered using comparative genomic analysis,which may aid in the evolution of the DA biosynthesis pathway. We identified several genes involved in DA biosynthesis via integrated genomic, transcriptomic, and metabolomic analyses. These genes included enzymes encoding target ent-kaurene oxidases and aminotransferases, which facilitated the activation of diterpenes and insertion of nitrogen atoms into diterpene skeletons, thereby mediating the transformation of diterpenes into DAs. The divergence periods of these genes in A. vilmorinianum were further assessed, and it was shown that two major types of genes were involved in the establishment of the DA biosynthesis pathway. Our integrated analysis offers fresh insights into the evolutionary origin of DAs in A.vilmorinianum as well as suggestions for engineering the biosynthetic pathways to obtain desired DAs. | Dake Zhao Ya Zhang Huanxing Ren Yana Shi Ding Dong Zonghang Li Guanghong Cui Yong Shen Zongmin Mou Edward JKennelly Luqi Huang Jue Ruan Suiyun Chen Diqiu Yu Yupeng Cun | 2023 | Journal of Integrative Plant Biology2023，65，10： | 0 |
| 18 | P75 and phosphorylated c-Jun are differentially regulated in spinal motoneurons following axotomy in rats显示文摘The neurotrophin receptor (p75) activates the c-Jun N-terminal kinase (JNK) pathway. Activation of JNK and its substrate c-Jun can cause apoptosis. Here we evaluate the role of p75 in spinal motoneurons by comparing immunoreactivity for p75 and phosphorylated c-Jun (p-c-Jun), the production of JNK activation in axotomized motoneurons in postnatal day (PN)1, PN7, PN14 and adult rats. Intensive p-c-Jun was induced in axotomized motoneurons in PN1 and PN7. In PN14, p-c-Jun expression was sharply reduced after the same injury. The decreased expression of p-c-Jun at this age coincided with a developmental switch of re-expression of p75 in axotomized cells. In adult animals, no p-c-Jun but intensive p75 was detected in axotomized motoneurons. These results indicate differential expression or turnover of phosphorylation of c-Jun and p75 in immature versus mature spinal motoneurons in response to axonal injury. The non-co-occurrence of p75 and p-c-Jun in injured motoneurons indicated that p75 may not activate JNK pathway, suggesting that the p75 may not be involved in cell death in axotomized motoneurons. | Qiuju Yuan Huanxing Su Wutian Wu Zhi-Xiu Lin | 2012 | Neural Regeneration Research2012，7，26： | 0 |
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