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| 1 | Direct generation of ES-like cells from unmodified mouse embryonic fibroblasts by Oct4/Sox2/Myc/KIf4显示文摘 | Dajiang Qin Wen Li Jin Zhang Duanqing Pei | 2007 | Cell Research2007，17，11： | 26 |
| 2 | BMPs functionally replace KLf4, and support efficient reprogramming of mouse fibroblasts by Oct4 alone显示文摘由定义因素的导致的 pluripotent 干细胞的产生成为了一个有用模型调查 reprogramming 和房间命运决心的机制。然而,基于因素的 reprogramming 的精确机制仍然保持不清楚。这里,我们证明 Klf4 主要在 reprogramming 的起始的阶段行动开始 mesenchymal-to-epithelial 转变并且能是机能上地由骨头代替了形态基因的蛋白质(BMP ) 。BMP 增加了老鼠的 Oct4/Sox2-mediated reprogramming 的效率胚胎的成纤维细胞(MEF ) 到 ~1% 。BMP 也支持了 MEF 和尾巴的单个因素的基于 Oct4 的 reprogramming 胫骨的成纤维细胞。我们的研究在 reprogramming 澄清 Klf4 的贡献并且在区分的房间作为 pluripotency 的单个 setter 建立 Oct4。 | Jiekai Chen Jing Liu Jiaqi Yang You Chen Jing Chen Su Ni Hong Song Lingwen Zeng Ke Ding Duanqing Pei | 2011 | Cell Research2011，21，1： | 26 |
| 3 | Lithium, an anti-psychotic drug, greatly enhances the generation of induced pluripotent stem cells显示文摘体的房间能由定义因素是进导致的 pluripotent 干细胞(iPSCs ) 的 reprogrammed。reprogramming 的低效率和 oncogenes 和病毒的向量的 genomic 集成限制了 iPSCs 的潜在的申请。这里,我们报导那锂(李) ,药过去常对待心情混乱,极大地从老鼠提高 iPSC 产生胚胎的成纤维细胞和人的脐的静脉 endothelial 房间。李与(Oct4 ) 或二个因素(OS 或好) 便于 iPSC 产生。支持 reprogramming 上的李的效果仅仅部分取决于它的主要目标 GSK3β。不同于另外的 GSK3β禁止者,李不仅增加 Nanog 的表示,而且提高 Nanog 的 transcriptional 活动。我们也发现李由经由 LSD1 的 downregulation 支持 epigenetic 修正施加它的效果, H3K4 特定的 histone demethylase。将 LSD1 击倒部分在提高 reprogramming 模仿李的效果。我们的结果不仅提供一个直接方法改进 iPSC 产生效率,而且为体的房间 reprogramming 作为一个批评调节的人识别了 histone demethylase。 | Quan Wang Xinxiu Xu Jun Li Jing Liu Haifeng Gu Ru Zhang Jiekai Chen Yin Kuang Jian Fei Cong Jiang Ping Wang Duanqing Pei Sheng Ding Xin Xie | 2011 | Cell Research2011，21，10： | 23 |
| 4 | The p53-induced lincRNA-p21 derails somatic cell reprogramming by sustaining H3K9me3 and CpG methylation at pluripotency gene promoters显示文摘最近的研究在众多的生物过程增加了我们长 noncoding RNA (lncRNAs ) 的理解,但是很少在体的房间 reprogramming 检验了他们的角色。通过表示介绍并且功能的屏蔽,我们鉴别了大 intergenic noncoding RNA p21 (lincRNA-p21 ) 损害 reprogramming。尤其是, lincRNA-p21 被 p53 导致,但是不在 reprogramming 支持 apoptosis 或房间老朽。相反, lincRNA-p21 与 H3K9 methyltransferase SETDB1 和维护 DNA methyltransferase DNMT1 联系,它被 RNA 有约束力的蛋白质 HNRNPK 便于。因而, lincRNA-p21 由在 pluripotency 基因倡导者支撑 H3K9me3 或 CpG methylation 阻止 reprogramming。我们的结果在 reprogramming 提供卓见进 lncRNAs 的角色并且建立在 p53 和 heterochromatin 规定之间的一个新奇连接。 | Xichen Bao Haitao Wu Xihua Zhu Xiangpeng Guo Andrew P Hutchins Zhiwei Luo Hong Song Yongqiang Chen Keyu Lai Menghui Yin Lingxiao Xu Liang Zhou Jiekai Chen Dongye Wang Baoming Qin Jon Frampton Hung-Fat Tse Duanqing Pei Huating Wang Biliang Zhang Miguel A Esteban | 2015 | Cell Research2015，25，1： | 21 |
| 5 | Special Section:SARS-CoV-2 Preliminary evidence from a multicenter prospective observational study of the safety and efficacy of chloroquine for the treatment of COVID-19显示文摘Effective therapies are urgently needed for the SARS-CoV-2 pandemic.Chloroquine has been proved to have antiviral effect against coronavirus in vitro.In this study,we aimed to assess the efficacy and safety of chloroquine with different doses in COVID-19.In this multicenter prospective observational study,we enrolled patients older than 18 years old with confirmed SARS-CoV-2 infection excluding critical cases from 12 hospitals in Guangdong and Hubei Provinces.Eligible patients received chloroquinephosphate s00 mg,orally,once(half dose)or twice(full dose)daily.Patients treated with non-chloroquine therapy were included as historical controls.The primary endpoint is the time to undetectable viral RNA.Secondary outcomes include the proportion of patients with undetectable viral RNA by day 10 and 14,hospitalization time,duration of fever,and adverse events.A total of 197 patients completed chloroquine treatment,and 176patients were included as historical controls.The median time to achieve an undetectable viral RNA was shorter in chloroquine than in non-chloroquine(absolute difference in medians-6.0 days;95%CI-6.0 to—4.0).The duration of fever is shorter in chloroquine(geometric mean ratio 0.6;95%CIO.5 to 0.8).No serious adverse events were observed in the chloroquine group.Patients treated with half dose experienced lower rate of adverse events than with full dose.Although randomized trials are needed for further evaluation,this study provides evidence for safety and efficacy of chloroquine in COVID-19 and suggests that chloroquine can be a cost-effective therapy for combating the COVID-19 pandemic. | Mingxing Huang Man Li Fei Xiao Pengfei Pang Jiabi Liang Tiantian Tang Shaoxuan Liu Binghui Chen Jingxian Shu Yingying You Yang Li Meiwen Tang Jianhui Zhou Guanmin Jiang Jingfen Xiang Wenxin Hong Songmei He Zhaoqin Wang Jianhua Feng Changqing Lin Yinong Ye Zhilong Wu Yaocai Li Bei Zhong Ruilin Sun Zhongsi Hong Jing Liu Huili Chen Xiaohua Wang Zhonghe Li Duanqing Pei Lin Tian Jinyu Xia Shanping Jiang Nanshan Zhong Hong Shan | 2020 | National Science Review2020，7，9： | 20 |
| 6 | Generation of RAG 1- and 2-deficient rabbits by embryo microinjection of TALENs显示文摘 | Jun Song Juan Zhong Xiaogang Guo Yongqiang Chen Qingjian zou Jiao Huang Xiaoping Li Quanjun Zhang Zhiwu Jiang Chengcheng Tang Huaqiang Yang Tao Liu Peng Li Duanqing Pei Liangxue Lai | 2013 | Cell Research2013，23，8： | 15 |
| 7 | The magic of four: induction of pluripotent stem cells from somatic cells by Oct4, Sox2, Myc and Klf4显示文摘到一个成年成年人的从一个受精卵的发展过程与显著精确性被规划。当受精卵和它的子孙体细胞的基因信息是一样时,它是一样的染色体的选择表情产生不同房间在一个成年人打的 200 左右。这些成年房间的区分的状态 epigenetically 被维持,大概通过染色质的修正并且联系嘘。在更高的哺乳动物,区别过程是不可逆的直到多丽的成功的克隆,这被认为[1 ] 。由在乳腺从一个充分区分的房间转移一个原子核, Wilmut 和同事能产生一个更高的哺乳动物的一件准确复制品,多丽[1 ] 。这个工作不仅证明区分的细胞的染色体能是进一个胚胎的状态的 reprogrammed 然后处理恢复 full-fledgeddevelopmental 产生一个正常成年人,而且恢复了动物克隆的地。从一个病人的一个体细胞可以是到胚胎的状态并且随后的 reprogrammed 的前景导致了“叫的治疗学的克隆”产生合适的一种特定的细胞类型因为移植治疗鼓励了一个新研究方向[2 ] 。然而,与“治疗学的克隆”联系的技术困难和道德的担心在诊所在它的最终的成功上扔了疑问。 | Huayu Qi Duanqing Pei, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, China pei_duanqing@gibh.ac.cn | 2007 | Cell Research2007，17，7： | 12 |
| 8 | Piglets cloned from induced pluripotent stem cells显示文摘 | Nana Fan Jijun Chen Zhouchun Shang Hongwei Dou Guangzhen Ji Qingjian Zou Lu Wu Lixiazi He Fang Wang Kai Liu Na Liu Jianyong Han Qi Zhou Dengke Pan Dongshan Yang Bentian Zhao Zhen Ouyang Zhaoming Liu Yu Zhao Lin Lin Chongming Zhong Quanlei Wang Shouqi Wang Ying Xu Jing Luan Yu Liang Zhenzhen Yang Jing Li Chunxia Lu Gabor Vajta Ziyi Li Hongsheng Ouyang Huayan Wang Yong Wang Yang Yang Zhonghua Liu Hong Wei Zhidong Luan Miguel A Esteban Hongkui Deng Huanming Yang Duanqing Pei Ning Li Gang Pei Lin Liu Yutao Du Lei Xiao Liangxue Lai | 2013 | Cell Research2013，23，1： | 12 |
| 9 | Rational optimization of reprogramming culture conditions for the generation of induced pluripotent stem cells with ultra-high efficiency and fast kinetics显示文摘几个抄写因素的宫外的表示能恢复胚胎的房间命运到有教养的体的房间并且产生导致的 pluripotent 干细胞(iPSCs ) ,揭示到 pluripotency 的一条以前未知的小径。然而,这种技术被低效率,慢动力学和多因素的要求当前限制。这里,我们证明 reprogramming 能被优化文化条件改进并且戏剧性地加速了。首先,我们开发了优化定义媒介, iCD1,它允许 Oct4/Sox2/Klf4 (OSK ) 完成的调停的 reprogramming 超离频效率(~10% 在白天 8 点) 。我们也发现这个优化条件使 Sox2 和 Klf4 变为非必需,尽管这二个因素的消除导致更低的效率和更慢的动力学。我们的研究定义一条弄短的线路,两个在预定和因素要求,向 pluripotency。这个新范例不仅提供一个基本原理进一步改进 iPSC 产生而且由定义因素简化 reprogramming 的概念的理解。 | Jiekai Chen Jing Liu You Chen Jiaqi Yang Jing Chen He Liu Xiangjie Zhao Kunlun Mo Hong Song Lin Guo Shilong Chu Deping Wang Ke Ding Duanqing Pei | 2011 | Cell Research2011，21，6： | 9 |
| 10 | Leukolysin/MMP25/MT6-MMP: a novel matrix metalloproteinase specifically expressed in the leukocyte lineage显示文摘INTRODUCTIONMembersofthematrixmetajloproteinases(MMP)havebeenconsistentlyimplicatedinthedestructionofextracellularmatrix(ECM)undermanypathophysiologicalconditionsfromangiogenesistoajrthritis[1-5].Evolvedfrommodulardomainsandmotifsthroughexon-shuning... | PEI DUANQING(Department of Pharmacology, 6-120 Jackson Hall, 321Church St. S.E., University of Minnesota, Minneapolis,MN 55455, USA)Tel: 612-626-1468 Fax: 612-625-8408 E-mail: peixx003@tc. umn. edu | 1999 | Cell Research1999，9，4： | 6 |
| 11 | Transposable elements at the center of the crossroads between embryogenesis, embryonic stem cells, reprogramming,and long non-coding RNAs显示文摘Transposable elements(TEs) are mobile genomic sequences of DNA capable of autonomous and nonautonomous duplication. TEs have been highly successful,and nearly half of the human genome now consists of various families of TEs. Originally thought to be non-functional,these elements have been co-opted by animal genomes to perform a variety of physiological functions ranging from TE-derived proteins acting directly in normal biological functions, to innovations in transcription factor logic and influence on epigenetic control of gene expression. During embryonic development, when the genome is epigenetically reprogrammed and DNA-demethylated, TEs are released from repression and show embryonic stage-specific expression, and in human and mouse embryos, intact TEderived endogenous viral particles can even be detected. Asimilar process occurs during the reprogramming of somatic cells to pluripotent cells: When the somatic DNA is demethylated, TEs are released from repression. In embryonic stem cells(ESCs), where DNA is hypomethylated, an elaborate system of epigenetic control is employed to suppress TEs, a system that often overlaps with normal epigenetic control of ESC gene expression. Finally, many long non-coding RNAs(lnc RNAs) involved in normal ESC function and those assisting or impairing reprogramming contain multiple TEs in their RNA. These TEs may act as regulatory units to recruit RNA-binding proteins and epigenetic modifiers. This review covers how TEs are interlinked with the epigenetic machinery and lnc RNAs, and how these links influence each other to modulate aspects of ESCs,embryogenesis, and somatic cell reprogramming. | Andrew Paul Hutchins Duanqing Pei | 2015 | Science Bulletin2015，60，20： | 5 |
| 12 | Transitions between epithelial and mesenchymal states during cell fate conversions显示文摘 | Xiang Li Duanqing Pei Hui Zheng | 2014 | Protein & Cell2014，5，8： | 4 |
| 13 | Generation of tooth-like structures from integration-free human urine induced pluripotent stem cells显示文摘Background:Tooth is vital not only for a good smile,but also good health.Yet,we lose tooth regularly due to accidents or diseases.An ideal solution to this problem is to regenerate tooth with patients’own cells.Here we describe the generation of tooth-like structures from integration-free human urine induced pluripotent stem cells(ifhU-iPSCs).Results:We first differentiated ifhU-iPSCs to epithelial sheets,which were then recombined with E14.5 mouse dental mesenchymes.Tooth-like structures were recovered from these recombinants in 3 weeks with success rate up to 30%for 8 different iPSC lines,comparable to H1 hESC.We further detected that ifhU-iPSC derived epithelial sheets differentiated into enamel-secreting ameloblasts in the tooth-like structures,possessing physical properties such as elastic modulus and hardness found in the regular human tooth.Conclusion:Our results demonstrate that ifhU-iPSCs can be used to regenerate patient specific dental tissues or even tooth for further drug screening or regenerative therapies. | Jinglei Cai Yanmei Zhang Pengfei Liu Shubin Chen Xuan Wu Yuhua Sun Ang Li Ke Huang Rongping Luo Lihui Wang Ying Liu Ting Zhou Shicheng Wei Guangjin Pan Duanqing Pei | 2013 | Cell Regeneration2013，2，1： | 4 |
| 14 | A SNX10/V-ATPase pathway regulates ciliogenesis in vitro and in vivo显示文摘排序 nexins (SNX )phosphoinositide 有约束力的蛋白质在在 vitro 排序各种各样的膜蛋白质被含有,但是在里面他们的 vivo 功能仍然保持大部分未知。我们以前报导 SNX10 是 SNX 家庭基因的一个独特成员因为它在房间举办 vacuolation 活动。我们在这研究由 loss-of-function 试金调查 SNX10 的生物功能并且证明 SNX10 在有教养的房间为主要的睫的形成被要求。在 zebrafish, SNX10 为内脏的机关的左右的 patterning 在 Kupffer 的泡和必需品涉及 ciliogenesis。机械学地, SNX10 与复杂的 V-ATPase 交往并且指向它到 ciliogenesis 被开始的中心体。象 SNX10 一样, V-ATPase 在 vitro 并且在 vivo 调整 ciliogenesis 并且对 SNX10 那么 synergistically 做。我们进一步发现 SNX10 和 V-ATPase 调整 Rab8a 的睫的 trafficking,它是睫的膜扩展的一个批评管理者。这些结果识别为 ciliogenesis 是关键的一条 SNX10/V-ATPase-regulated 小囊的 trafficking 小径,并且表明通过在各种各样的机关的睫形成的规定, SNX10/V-ATPase 在早胚胎的开发期间起一个必要作用。 | Yanqun Chen Bin Wu Liangliang Xu Huapeng Li Jianhong Xia Wenguang Yin Zhuo Li Dawei Shi Song Li Shuo Lin Xiaodong Shu Duanqing Pei | 2012 | Cell Research2012，22，2： | 4 |
| 15 | Rapid generation of ACE2 humanized inbred mouse model for COVID-19 with tetraploid complementation显示文摘Although monkeys and pigs can be infected with SARS-Co V-2,they generally have a long growth cycle and a large size that is difficult to handle in large quantities.However,the readily available rats and mice are not susceptible to SARSCo V-2 because of differences in ACE2(angiotensin-converting enzyme 2),the receptor mediating cell entry of SARSCo V-2[1].Thus,the key to establishing a mouse model of COVID-19 is to make the mice express human ACE2 protein,and therefore become SARS-Co V-2 susceptible.Recently reported COVID-19 mouse models using a random insertion transgene approach[2,3]or adenoviral approach to express h ACE2[4],lack the tissue-specificity of h ACE2 expression and might not replicate COVID-19 precisely,limiting their applications in certain studies. | Feng-Liang Liu Kaixin Wu Jiaoyang Sun Zilei Duan Xiongzhi Quan Junqi Kuang Shilong Chu Wei Pang Han Gao Ling Xu Ying-Chang Li Hai-Lin Zhang Xue-Hui ang Rong-Hua Luo Xiao-Li Feng Hans RScholer Xinwen Chen Duanqing Pei Guangming Wu Yong-Tang Zheng Jiekai Chen | 2021 | National Science Review2021，8，2： | 3 |
| 16 | Neural progenitor cells from human induced pluripotent stem cells generated less autogenous immune response显示文摘The breakthrough development of induced pluripotent stem cells(iPSCs)raises the prospect of patient-specific treatment for many diseases through the replacement of affected cells.However,whether iPSC-derived functional cell lineages generate a deleterious immune response upon auto-transplantation remains unclear.In this study,we differentiated five human iPSC lines from skin fibroblasts and urine cells into neural progenitor cells(NPCs)and analyzed their immunogenicity.Through co-culture with autogenous peripheral blood mononuclear cells(PBMCs),we showed that both somatic cells and iPSC-derived NPCs do not stimulate significant autogenous PBMC proliferation.However,a significant immune reaction was detected when these cells were co-cultured with allogenous PBMCs.Furthermore,no significant expression of perforin or granzyme B was detected following stimulation of autogenous immune effector cells(CD3+CD8 T cells,CD3+CD8+T cells or CD3 CD56+NK cells)by NPCs in both PBMC and T cell co-culture systems.These results suggest that human iPSC-derived NPCs may not initiate an immune response in autogenous transplants,and thus set a base for further preclinical evaluation of human iPSCs. | HUANG Ke LIU PengFei LI Xiang CHEN ShuBin WANG LiHui QIN Li SU ZhengHui HUANG WenHao LIU JuLi JIA Bei LIU Jie CAI JingLei PEI DuanQing PAN GuangJin | 2014 | Science China(Life Sciences)2014，57，2： | 3 |
| 17 | Where cell fate conversions meet Chinese philosophy显示文摘 | Hui Zheng Andrew Paul Hutchins Guangjin Pan Yinxiong Li Duanqing Pei Gang Pei | 2014 | Cell Research2014，24，10： | 2 |
| 18 | The magic continues for the iPS strategy显示文摘自从第一在 2006 由定义因素在老鼠成纤维细胞的 reprogramming 上从 Yamanaka 和同事报导进 pluripotent 干细胞,惊人进步被取得了[1, 2 ] 。首先,几个组独立地报导了从由 Nanogor Oct4- 的老鼠体细胞的 pluripotent 干细胞的推导基于选择策略并且证明 iPS 房间作为 ES 房间拥有几乎相同的性质[3-5 ] 。第二,没有药选择, iPS 房间能被产生,暗示 iPS 技术能被用于任何未修改的房间[6-8 ] 。第三,人的 iPS 房间被三个独立的组,包括 Oct4 的因素的令人惊讶地采用的不同联合, Sox2, Myc, Klf4, Nanog, Lin28, SV40L-T,和 HTERT 报导[9-11 ] 。最后,由房间从自体同源的皮肤导出的 iPS 的老鼠镰刀房间贫血症的成功的治疗被报导,铺平为在疾病治疗的 iPS 技术的申请的道路[12 ] 。这些进步在不到 6 个月的一个时期被报导,进一步表明 iPS 技术产生了的强烈兴趣和它提供了的大机会。这些快速的进步在不久的将来为 iPS 技术预见开发的加速的步。这里,我考察一些最近的突破并且在未来开发讨论他们的影响,特别在疾病治疗的潜在的申请。 | Duanqing Pei | 2008 | Cell Research2008，18，2： | 2 |
| 19 | EMT and MET as paradigms for cell fate switching显示文摘Cell fate determination is a major unsolved problem in cell and developmental biology.The discovery of reprogramming by pluri-potent factors offers a rational system to investigate the molecular mechanisms associated with cell fate decisions.The idea that reprogramming of fibroblasts starts with a mesenchymal-epithelial transition(MET)suggests that the process is perhaps a reversal of epithelial to mesenchymal transition(EMT)found frequently during early embryogenesis.As such,we believe that investigations into MET-EMT may yield detailed molecular insights into cell fate decisions,not only for the switching between epithelial and mesenchymal cells,but also other cell types. | Jiekai Chen Qingkai Han Duanqing Pei | 2012 | Journal of Molecular Cell Biology2012，4，2： | 2 |
| 20 | Immediate expression of Cdh2 is essential for efficient neural differentiation of mouse induced pluripotent stem cells显示文摘 | Huanxing Su Lihui Wang Wenhao Huang Dajiang Qin Jinglei Cai Xiaoli Yao Chengqian Feng Zhiyuan Li Yitao Wang Kwok-Fai So Guangjin Pan Wutian Wu Duanqing Pei | 2013 | Stem Cell Research2013，，： | 1 |
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