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| 1 | Portal vein thrombosis in cirrhosis: Controversies and latest developments显示文摘Portal vein thrombosis(PVT) is encountered in livercirrhosis, particularly in advanced disease. It has been a feared complication of cirrhosis, attributed to significant worsening of liver disease, poorer clinical outcomes and potential inoperability at liver transplantation; also catastrophic events such as acute intestinal ischaemia. Optimal management of PVT has not yet been addressed in any consensus publication.We review current literature on PVT in cirrhosis; its prevalence, pathophysiology, diagnosis, impact on the natural history of cirrhosis and liver transplantation,and management. Studies were identified by a search strategy using MEDLINE and Google Scholar. The incidence of PVT increases with increasing severity of liver disease: less than 1% in well-compensated cirrhosis, 7.4%-16% in advanced cirrhosis. Prevalence in patients undergoing liver transplantation is 5%-16%.PVT frequently regresses instead of uniform thrombus progression. PVT is not associated with increased risk of mortality. Optimal management has not been addressed in any consensus publication. We propose areas for future research to address unresolved clinical questions. | Damian J Harding M Thamara PR Perera Frederick Chen Simon Olliff Dhiraj Tripathi | 2015 | World Journal of Gastroenterology2015，21，22： | 42 |
| 2 | Cytotoxic genes from traditional Chinese medicine inhibit tumor growth both in vitro and in vivo显示文摘OBJECTIVE: Little effort has been made to study the protein-encoding genes isolated from traditional Chinese medicine(TCM) drugs, and the delivery of these genes into malignant cells through recombinant adeno-associated virus(r AAV) vectors has not been attempted. METHODS: We synthesized the c DNAs of five known cytotoxic proteins isolated from TCM drugs and the FLAG epitope-tagged c DNAs were subcloned into a r AAV plasmid vector. The protein expression was confi rmed by Western blot assay. Various cancer cell lines were transfected with the above plasmids and cell growth was monitored both in vitro and in vivo. The best cytotoxic gene was further packaged into r AAV vectors, under the control of a liver cancer-specifi c promoter. The liver tumor growth was then monitored following intratumor administration of the r AAV vectors.RESULTS: The expression plasmids, encoding individual potential cytotoxic genes tagged with FLAG epitope, were successfully generated and sequenced. Among these genes, trichosanthin(TCS) gene yielded the most promising results for the inhibition of cancer cell growth in vitro. The over-expressed TCS functioned as a type I ribosome-inactivating protein, followed by inducing apoptosis that is associated with the Bcl-PARP signaling pathway. Furthermore, intratumor injection of r AAV vectors containing the TCS gene signifi cantly inhibited the growth of human hepatocellular carcinoma tumors in a murine xenograft model.CONCLUSION: Our studies suggest that the use of TCM cytotoxic genes is a useful therapeutic strategy for treating human cancers in general, and liver tumors in particular. | Yuan-hui Zhang Yuan Wang Ali Hussein Yusufali Frederick Ashby Daniel Zhang Zi-fei Yin George V.Aslanidi Arun Srivastava Chang-quan Ling Chen Ling | 2014 | Journal of Integrative Medicine2014，12，6： | 14 |
| 3 | A method for the production and expedient screening of CRISPR/Cas9-mediated non-transgenic mutant plants显示文摘Developing CRISPR/Cas9-mediated non-transgenic mutants in asexually propagated perennial crop plants is challenging but highly desirable.Here,we report a highly useful method using an Agrobacterium-mediated transient CRISPR/Cas9 gene expression system to create non-transgenic mutant plants without the need for sexual segregation.We have also developed a rapid,cost-effective,and high-throughput mutant screening protocol based on Illumina sequencing followed by high-resolution melting(HRM)analysis.Using tetraploid tobacco as a model species and the phytoene desaturase(PDS)gene as a target,we successfully created and expediently identified mutant plants,which were verified as tetra-allelic mutants.We produced pds mutant shoots at a rate of 47.5%from tobacco leaf explants,without the use of antibiotic selection.Among these pds plants,17.2%were confirmed to be non-transgenic,for an overall non-transgenic mutation rate of 8.2%.Our method is reliable and effective in creating non-transgenic mutant plants without the need to segregate out transgenes through sexual reproduction.This method should be applicable to many economically important,heterozygous,perennial crop species that are more difficult to regenerate. | Longzheng Chen Wei Li Lorenzo Katin-Grazzini Jing Ding Xianbin Gu Yanjun Li Tingting Gu Ren Wang Xinchun Lin Ziniu Deng Richard J.McAvoy Frederick G.Gmitter Jr. Zhanao Deng Yunde Zhao Yi Li | 2018 | Horticulture Research2018，5，1： | 12 |
| 4 | Reprogramming of a defense signaling pathway in rough lemon and sweet orange is a critical element of the early response to‘Candidatus Liberibacter asiaticus’显示文摘Huanglongbing(HLB)in citrus infected by Candidatus Liberibacter asiaticus(CLas)has caused tremendous losses to the citrus industry.No resistant genotypes have been identified in citrus species or close relatives.Among citrus varieties,rough lemon(Citrus jambhiri)has been considered tolerant due to its ability to produce a healthy flush of new growth after infection.The difference between tolerance and susceptibility is often defined by the speed and intensity of a plant’s response to a pathogen,especially early defense responses.RNA-seq data were collected from three biological replicates of CLas-and mock-inoculated rough lemon and sweet orange at week 0 and 7 following infection.Functional analysis of the differentially expressed genes(DEGs)indicated that genes involved in the mitogen activated protein kinase(MAPK)signaling pathway were highly upregulated in rough lemon.MAPK induces the transcription of WRKY and other transcription factors which potentially turn on multiple defense-related genes.A Subnetwork Enrichment Analysis further revealed different patterns of regulation of several functional categories,suggesting DEGs with different functions were subjected to reprogramming.In general,the amplitude of the expression of defense-related genes is much greater in rough lemon than in sweet orange.A quantitative disease resistance response may contribute to the durable tolerance level to HLB observed in rough lemon. | Qibin Yu Chunxian Chen Dongliang Du Ming Huang Jiqiang Yao Fahong Yu Ronald H Brlansky Frederick G.Gmitter Jr | 2017 | Horticulture Research2017，4，1： | 5 |
| 5 | Resistance switching for RRAM applications显示文摘Resistive random access memory (RRAM or ReRAM) is a non-volatile memory (NVM) technology that consumes minimal energy while offering sub-nanosecond switching. In addition, the data stability against high temperature and cycling wear is very robust, allowing new NVM applications in a variety of markets (au- tomotive, embedded, storage, RAM). Based on sudden conduction through oxide insulators, the characteristics of RRAM technology have still yet to be fully described. In this paper, we present our current understanding of this very promising technology. | CHEN Frederick T LEE HengYuan CHEN YuSheng HSU YenYa ZHANG LiJie CHEN PangShiu CHEN WeiSu GU PeiYi LIU WenHsing WANG SuMin TSAI ChenHan SHEU ShyhShyuan TSAI MingJinn HUANG Ru | 2011 | Science China(Information Sciences)2011，54，5： | 3 |
| 6 | Use of angiotensin-converting enzyme inhibitors and angiotensin Ⅱ receptor blockers in context of COVID-19 outbreak:a retrospective analysis显示文摘The possible effects of angiotensin-converting enzyme inhibitors(ACEIs)or angiotensin Ⅱ receptor blockers(ARBs)on COVID-19 disease severity have generated considerable debate.We performed a single-center,retrospective analysis of hospitalized adult COVID-19 patients in Wuhan,China,who had definite clinical outcome(dead or discharged)by February 15,2020.Patients on anti-hypertensive treatment with or without ACEI/ARB were compared on their clinical characteristics and outcomes.The medical records from 702 patients were screened.Among the 101 patients with a history of hypertension and taking at least one anti-hypertensive medication,40 patients were receiving ACEI/ARB as part of their regimen,and 61 patients were on antihypertensive medication other than ACEI/ARB.We observed no statistically significant differences in percentages of in-hospital mortality(28%vs.34%,P=0.46),ICU admission(20%vs.28%,P=0.37)or invasive mechanical ventilation(18%vs.26%,P=0.31)between patients with or without ACEI/ARB treatment.Further multivariable adjustment of age and gender did not provide evidence for a significant association between ACEI/ARB treatment and severe COVID-19 outcomes.Our findings confirm the lack of an association between chronic receipt of reninangiotensin system antagonists and severe outcomes of COVID-19.Patients should continue previous antihypertensive therapy until further evidence is available. | Jiuyang Xu Chaolin Huang Guohui Fan Zhibo Liu Lianhan Shang Fei Zhou Yeming Wang Jiapei Yu Luning Yang Ke Xie Zhisheng Huang Lixue Huang Xiaoying Gu Hui Li Yi Zhang Yimin Wang Frederick G.Hayden Peter W.Horby Bin Cao Chen Wang | 2020 | Frontiers of Medicine2020，14，5： | 2 |
| 7 | MEK inhibitor PD0325901 significantly reduces the growth of papillary thyroid carcinoma cells in vitro and in vivo显示文摘 | Henderson YC Chen Y Frederick MJ | 2010 | Mol Cancer Ther2010，9，7： | 1 |
| 8 | Proteomic and metabolomic analyses provide insight into the off-flavour of fruits from citrus trees infected with‘Candidatus Liberibacter asiaticus’显示文摘Orange fruit from trees infected by‘Candidatus Liberibacter asiaticus’(CaLas)often do not look fully mature and exhibit off-flavours described as bitter,harsh,and metallic rather than juicy and fruity.Although previous studies have been carried out to understand the effect of CaLas on the flavour of orange juice using metabolomic methods,the mechanisms leading to the off-flavour that occurs in Huanglongbing(HLB)-symptomatic fruit are not well understood.In this study,fruits were collected from symptomatic and healthy Valencia sweet orange(Citrus sinensis)trees grafted on Swingle(C.paradisi X Poncirus trifoliata)rootstock.Isobaric tags for relative and absolute quantification(iTRAQ)and gas chromatography-mass spectrometry(GC-MS)were used to measure the proteins,sugars,organic acids,amino acids,and volatile terpenoids.The results showed that most of the differentially expressed proteins involved in glycolysis,the tricarboxylic acid(TCA)cycle and amino-acid biosynthesis were degraded,and terpenoid metabolism was significantly downregulated in the symptomatic fruit.Valencene,limonene,3-carene,linalool,myrcene,andα-terpineol levels were significantly lower in fruit from CaLas-infected trees than from healthy trees.Similar phenomena were observed for sucrose and glucose.Our study indicated that off-flavour of symptomatic fruit was associated with a reduction in the levels of terpenoid products and the downregulation of proteins in glycolysis,the TCA cycle,and the terpenoid biosynthesis pathway. | Lixiao Yao Qibin Yu Ming Huang Weilun Hung Jude Grosser Shanchun Chen Yu Wang Frederick G.GmitterJr. | 2019 | Horticulture Research2019，6，1： | 1 |
| 9 | Winning probabilities in a pairwise lottery system with three alternatives显示文摘 | CHEN FREDERICK H JAC C HECKELMAN | 2005 | Economic Theory2005，26，3： | 1 |
| 10 | Electrocardiographic findings in Emergency Department patients with pulmonary embolism显示文摘 | Peter B Richman Hassan Loutfi Steven J Lester Patricia Cambell Jessica Matthews Jeremy Friese Joseph Wood David Kasper Frederick Chen Mark Mandell | 2004 | Journal of Emergency Medicine2004，，2： | 1 |
| 11 | Barrett's esophagus and adenocarcinoma显示文摘 | Chen MY Frederick MG | 1994 | Radiol Clin North Am1994，32，： | 1 |
| 12 | Outcomes of pregnancy in patients with known Budd-Chiari syndrome显示文摘AIM To analyse the risk of pregnancy(a prothrombotic state) in patients with Budd-Chiari Syndrome(BCS). METHODS Retrospective study of pregnancy in women with known BCS at single center from January 2001 to December 2015. RESULTS Out of 53 females with BCS, 7 women had 16 pregnancies. Median age at diagnosis of BCS in these women was 25 years(range 21-34 years). At least one causal factor for BCS was identified in 6 women(86%). Six women had undergone radiological decompressive treatment. All patients had anticoagulation. Six fetuses were lost before 20 wk gestation in 2 women. There were 9 deliveries over 32 wk gestation and one delivery at 27 wk. All infants did well. Seven babies were born by emergency caesarean section. There were no cases of thrombosis. Two patients had notable vaginal(PV) bleeding in 3 pregnancies. None of the patients had variceal haemorrhage. Two patients were diagnosed with pulmonary hypertension, one during pregnancyand the other in the post-partum period. There was no maternal mortality.CONCLUSION Maternal outcomes in patients with treated BCS are favourable and fetal outcomes beyond 20 wk gestation are good. There has been increased rate of caesarean section. Pulmonary hypertension is an important finding that needs further validation. These patients should be managed in centers experienced in treating high-risk pregnancies. | Faisal Khan Ian Rowe Bill Martin Ellen Knox Tracey Johnston Charlie Elliot Will Lester Frederick Chen Simon Olliff Homoyon Mehrzad Zergham Zia Dhiraj Tripathi | 2017 | World Journal of Hepatology2017，9，21： | 1 |
| 13 | Off-pump coronary artery bypass grafting decreases risk-adjusted mortality and morbidity显示文摘 | Joseph C Cleveland A.Laurie W Shroyer Anita Y Chen Eric Peterson Frederick L Grover | 2001 | The Annals of Thoracic Surgery2001，，4： | 1 |
| 14 | Ezrin, a membrane-cytoskeletal linking protein, is highly expressed in atypical endometrial hyperplasia and uterine endometrioid adenocarcinoma显示文摘 | Kaori Ohtani Hideki Sakamoto Thomas Rutherford Zhaocong Chen Atushi Kikuchi Tatsuo Yamamoto Kazuo Satoh Frederick Naftolin | 2002 | Cancer Letters2002，，1： | 1 |
| 15 | The effect of silica nanoparticle-modified surfaces on cell morphology, cytoskeletal organization and function显示文摘 | Anna M. Lipski Christopher J. Pino Frederick R. Haselton I.-Wei Chen V. Prasad Shastri | 2008 | Biomaterials2008，，： | 1 |
| 16 | Occurrence and persistence of erythromycin resistance genes (erm) and tetracycline resistance genes (tet) in waste treatment systems on swine farms显示文摘 | Chen J Frederick C Michel J R | 2010 | Microb Ecol2010，60，： | 1 |
| 17 | Early and late outcomes of 1000 minimally invasive aortic valve operations显示文摘 | Minoru Tabata Ramanan Umakanthan Lawrence H. Cohn Ralph Morton Bolman Prem S. Shekar Frederick Y. Chen Gregory S. Couper Sary F. Aranki | 2008 | European Journal of Cardio-Thoracic Surgery2008，，4： | 1 |
| 18 | Development and application of a decision group Back-Propagation Neural Network for flood forecasting显示文摘 | Chang-Shian Chen Boris Po-Tsang Chen Frederick Nai-Fang Chou Chao-Chung Yang | 2010 | Journal of Hydrology2010，，1： | 1 |
| 19 | Mechanisms of tolvaptan-induced toxicity in HepG2 cells显示文摘 | Yuanfeng Wu Frederick A. Beland Si Chen Fang Liu Lei Guo Jia-Long Fang | 2015 | Biochemical Pharmacology2015，，4： | 1 |
| 20 | MEK inhibitor PD0325901 significantly reduces the growth of papillary thyroid carcinoma cells in vitro and in vivo显示文摘 | Henderson YC Chen Y Frederick MJ | 2010 | Mol Cancer Ther2010，9，7： | 1 |
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