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| 1 | Expression of nuclear factor-KB in hepatocellular carcinoma and its relation with the X protein of hepatitis B virus显示文摘AIM In this study we investigated therelationship of the X protein of HBV and nuclearfactor-KB (NF-κB) and the expression of NF-KB inhuman hepatocellular carcinoma tissues.METHODS Immunohistochemistry SP methodwas used to detect the expression of NF-κB and the X protein of HBV in human hepatocellularcarcinoma tissues of 52 cases. Gene transfectionmediated by lipofectamine was used to transfectthe eukaryotic expression vector pCDNA3. 1-HBXof HBV x gene into human hepatocellularcarcinoma cell line HCC-9204 and NF-κB wasdetected.RESULTS NF-κB was widely expressed inhuman hepatocellular carcinoma tissues in atotal of 52 cases and its expression was relatedto the X protein of HBV. NF-KB was localizedboth in the cytoplasm and . The nuclei ofhepatocellular carcinoma cells in 11 cases whichwere positive for the X protein of HBV while in 41cases negative for the X protein of HBV, NF-Kbwas only localized in the cytoplasm ofhepatocellular carcinoma cells but translocatedto the nuclei of hepatocellular carcinoma cellsafter the eukaryotic expression vectorpCDNA3.1-HBX was transfected into HCC-9204cells.CONCLUSION This study strongly suggeststhat the nuclear factor NF-KB is widely expressedin hepatocellular carcinoma tissues in differentstyles according to the expression of the Xprotein of HBV. NF-κB is abnormally activated inhepatocellular carcinoma, which is probablyrelated to the X protein of HBV. The X protein ofHBV can activate NF-κB to translocate into nucleiof hepatocellular carcinoma cells. | Shuang Ping Guo~1 Wen Liang Wang~1 Yu Qiang Zhai~2 Yi Ling Zhao~1 ~1Department of Pathology,Xijing Hospital of the Fourth Military Medical University,Xi’an,China ~2Department of Urology,the Central Hospital of Xi’an,China | 2001 | World Journal of Gastroenterology2001，7，3： | 55 |
| 2 | 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents显示文摘Syncope belongs to the transient loss of consciousness(TLOC), characterized by a rapid onset, short duration, and spontaneous complete recovery. It is common in children and adolescents, accounting for 1% to 2% of emergency department visits.Recurrent syncope can seriously affect children's physical and mental health, learning ability and quality of life and sometimes cardiac syncope even poses a risk of sudden death. The present guideline for the diagnosis and treatment of syncope in children and adolescents was developed for guiding a better clinical management of pediatric syncope. Based on the globally recent development and the evidence-based data in China, 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents was jointly prepared by the Pediatric Cardiology Society, Chinese Pediatric Society, Chinese Medical Association(CMA)/Committee on Pediatric Syncope, Pediatricians Branch, Chinese Medical Doctor Association(CMDA)/Committee on Pediatric Cardiology, Chinese College of Cardiovascular Physicians, Chinese Medical Doctor Association(CMDA)/Pediatric Cardiology Society, Beijing Pediatric Society, Beijing Medical Association(BMA). The present guideline includes the underlying diseases of syncope in children and adolescents, the diagnostic procedures, methodology and clinical significance of standing test and headup tilt test, the clinical diagnosis vasovagal syncope, postural orthostatic tachycardia syndrome, orthostatic hypotension and orthostatic hypertension, and the treatment of syncope as well as follow-up. | Cheng Wang Yaqi Li Ying Liao Hong Tian Min Huang Xiangyu Dong Lin Shi Jinghui Sun Hongfang Jin Junbao Du Jindou An Jie Chen Mingwu Chen Qi Chen Sun Chen Yonghong Chen Zhi Chen Adolphus Kai-tung Chau Junbao Du Zhongdong Du Junkai Duan Hongyu Duan Xiangyu Dong Lin Feng Lijun Fu Fangqi Gong Yonghao Gui Ling Han Zhenhui Han Bing He Zhixu He Xiufen Hu Yimin Hua Guoying Huang Min Huang Ping Huang Yujuan Huang Hongfang Jin Mei Jin Bo Li Fen Li Tao Li Xiaohui Li Xiaoyan Liu Yan Li Haitao Lv Tiewei Lv Zipu Li Luyi Ma Silin Pan Yusheng Pang Hua Peng Yuming Qin Jie Shen Lin Shi Kun Sun Jinghui Sun Hong Tian Jie Tian Cheng Wang Hong Wang Lei Wang Jinju Wang Wendi Wang Yuli Wang Rongzhou Wu Tianhe Xia Yanyan Xiao Chunhong Xie Yanlin Xing Zhenyu Xiong Baoyuan Xu Yi Xu Hui Yan Shiwei Yang Qijian Yi Xia Yu Xianyi Yu Yue Yuan Hongyan Zhang Huili Zhang Li Zhang Qingyou Zhang Xi Zhang Yanmin Zhang Zhiwei Zhang Cuifen Zhao Bin Zhou Hua Zhu | 2018 | Science Bulletin2018，63，23： | 54 |
| 3 | The Reference Genome of Tea Plant and Resequencing of 81 Diverse Accessions Provide Insights into Its Genome Evolution and Adaptation显示文摘Tea plant is an important economic crop,which is used to produce the world's oldest and most widely consumed tea beverages.Here,we present a high-quality reference genome assembly of the tea plant(Camellia sinensis var.sinensis)consisting of 15 pseudo-chromosomes.LTR retrotransposons(LTR-RTs)account for 70.38%of the genome,and we present evidence that LTR-RTS play critical roles in genome size expansion and the transcriptional diversification of tea plant genes through preferential insertion in promoter regions and introns.Genes,particularly those coding for terpene biosynthesis pro-teins,associated with tea aroma and stress resistance were significantly amplified through recent tandem duplications and exist as gene clusters in tea plant genome.Phylogenetic analysis of the sequences of 81 tea plant accessions with diverse origins revealed three well-differentiated tea plant populations,support-ing the proposition for the southwest origin of the Chinese cultivated tea plant and its later spread to western Asia through introduction.Domestication and modern breeding left significant signatures on hundreds of genes in the tea plant genome,particularly those associated with tea quality and stress resis-tance.The genomic sequences of the reported reference and resequenced tea plant accessions provide valuable resources for future functional genomics study and molecular breeding of improved cul-tivars of tea plants. | Enhua Xia Wei Tong Yan Hou Yanlin An Linbo Chen Qiong Wu Yunlong Liu Jie Yu Fangdong Li Ruopei Li Penghui Li Huijuan Zhao Ruoheng Ge Jin Huang Ali Inayat Mallano Yanrui Zhang Shengrui Liu Weiwei Deng Chuankui Song Zhaoliang Zhang Jian Zhao Shu Wei Zhengzhu Zhang Tao Xia Chaoling Wei Xiaochun Wan | 2020 | Molecular Plant2020，13，7： | 51 |
| 4 | Moment-independent importance measure of basic random variable and its probability density evolution solution显示文摘To analyze the effect of basic variable on failure probability in reliability analysis,a moment-independent importance measure of the basic random variable is proposed,and its properties are analyzed and verified.Based on this work,the importance measure of the basic variable on the failure probability is compared with that on the distribution density of the response.By use of the probability density evolution method,a solution is established to solve two importance measures,which can efficiently avoid the difficulty in solving the importance measures.Some numerical examples and engineering examples are used to demonstrate the proposed importance measure on the failure probability and that on the distribution density of the response.The results show that the proposed importance measure can effectively describe the effect of the basic variable on the failure probability from the distribution density of the basic variable.Additionally,the results show that the established solution on the probability density evolution is efficient for the importance measures. | CUI LiJie,Lü ZhenZhou & ZHAO XinPan School of Aeronautics,Northwestern Polytechnical University,Xi’an 710072,China | 2010 | Science China(Technological Sciences)2010，53，4： | 41 |
| 5 | Prevalence, risk factors, clinical course, and outcome of acute kidney injury in Chinese intensive care units: a prospective cohort study显示文摘 | WEN Ying JIANG Li XU Yuan QIAN Chuan-yun LI Shu-sheng QIN Tie-he CHEN Er-zhen LIN Jian-dong AI Yu- hang WU Da-wei WANG Yu-shan SUN Ren-hua HU Zhen-jie CAO Xiang-yuan ZHOU Fa-chun HE Zhen-yang ZHOU Li-hua AN You-zhong KANG Yan MA Xiao-chun YU Xiang-you ZHAO Ming-yan XI Xiu-ming DU Bin | 2013 | Chinese Medical Journal2013，，23： | 40 |
| 6 | Laparoscopic vs open D2 gastrectomy for locally advanced gastric cancer: A meta-analysis显示文摘AIM:To conduct a meta-analysis comparing laparoscopic(LGD2)and open D2 gastrectomies(OGD2)for the treatment of advanced gastric cancer(AGC).METHODS:Randomized controlled trials(RCTs)and non-RCTs comparing LGD2 with OGD2 for AGC treatment,published between 1 January 2000 and 12January 2013,were identified in the Pub Med,Embase,and Cochrane Library databases.Primary endpoints included operative outcomes(operative time,intraoperative blood loss,and conversion rate),postoperative outcomes(postoperative analgesic consumption,time to first ambulation,time to first flatus,time to first oralintake,postoperative hospital stay length,postoperative morbidity,incidence of reoperation,and postoperative mortality),and oncologic outcomes(the number of lymph nodes harvested,tumor recurrence and metastasis,disease-free rates,and overall survival rates).The Cochrane Collaboration tools and the modified Newcastle-Ottawa scale were used to assess the quality and risk of bias of RCTs and non-RCTs in the study.Subgroup analyses were conducted to explore the incidence rate of various postoperative morbidities as well as recurrence and metastasis patterns.A Begg’s test was used to evaluate the publication bias.RESULTS:One RCT and 13 non-RCTs totaling 2596patients were included in the meta-analysis.LGD2 in comparison to OGD2 showed lower intraoperative blood loss[weighted mean difference(WMD)=-137.87 m L,95%CI:-164.41--111.33;P<0.01],lower analgesic consumption(WMD=-1.94,95%CI:-2.50--1.38;P<0.01),shorter times to first ambulation(WMD=-1.03d,95%CI:-1.90--0.16;P<0.05),flatus(WMD=-0.98d,95%CI:-1.30--0.66;P<0.01),and oral intake(WMD=-0.85 d,95%CI:-1.67--0.03;P<0.05),shorter hospitalization(WMD=-3.08 d,95%CI:-4.38--1.78;P<0.01),and lower postoperative morbidity(odds ratio=0.78,95%CI:0.61-0.99;P<0.05).No significant differences were observed between LGD2 and OGD2 for the following criteria:reoperation incidence,postoperative mortality,number of harvested lymph nodes,tumor recurrence/metastasis,or three-or five-year diseasefree and overall survival rates.However,LGD2 had longer operative times(WMD=57.06 min,95%CI:41.87-72.25;P<0.01).CONCLUSION:Although a technically demanding and time-consuming procedure,LGD2 may be safe and effective,and offer some advantages over OGD2 for treatment of locally AGC. | Zhen-Hong Zou Li-Ying Zhao Ting-Yu Mou Yan-Feng Hu Jiang Yu Hao Liu Hao Chen Jia-Ming Wu Sheng-Li An Guo-Xin Li | 2014 | World Journal of Gastroenterology2014，20，44： | 25 |
| 7 | The global image of the Moon obtained by the Chang'E-1:Data processing and lunar cartography显示文摘The global lunar image of the first phase of Chinese Lunar Exploration Program is the first image that covered all over the surface of the Moon. It will serve as a critical foundation for succeeding exploration and scientific research. In this paper, the acquisition, characteristics, and data quality of Chang'E-1 CCD image data are described in detail. Also described are the methodology and procedure of data processing. According to rule of planetary cartography, the image data have been processed, geometrically corrected, and then mosaicked and merged in a scale of 1:2.5 million. The results of data processing and charting show that the image data of Chang'E-1 CCD and their geometric precision meet the demand of charting a map in the scale of 1:2.5 million. The relative geometric positioning precision of the global image is better than 240 m, and the absolute geometric positioning precision is slightly better than that of the ULCN2005 and Clementine lunar basemap (V2.0). The plane positioning precision is approximately 100-1500 m. This global image proves to be the best global image of the Moon so far in terms of space coverage, image quality, and positioning precision. | LI ChunLai1, LIU JianJun1, REN Xin1, MOU LingLi1, ZOU YongLiao1, ZHANG HongBo1, Lü Chang1, LIU JianZhong1, ZUO Wei1, SU Yan1, WEN WeiBin1, BIAN Wei1, ZHAO BaoChang2, YANG JianFeng2, ZOU XiaoDuan1, WANG Min1, XU Chun1, KONG DeQing1, WANG XiaoQian1, WANG Fang1, GENG Liang1, ZHANG ZhouBin1, ZHENG Lei1, ZHU XinYing1, LI JunDuo1 & OUYANG ZiYuan11 National Astronomical Observatories, Chinese Academy of Sciences, Beijing 100012, China 2 Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an 710119, China | 2010 | Science China Earth Sciences2010，53，8： | 22 |
| 8 | Triptolide induces Sertoli cell apoptosis in mice via ROS/JNK-dependent activation of the mitochondrial pathway and inhibition of Nrf2-mediated antioxidant response显示文摘Triptolide (TP ) ,氧化 diterpene,举办许多有益的 pharmacodynamic 活动但是它的临床的应用程序由于严重阴囊的损害被限制。这研究试图描出在 vitro 并且在 vivo 的导致 TP 的阴囊的损害的分子的机制。TP (5-50000 nmol/L ) dose-dependently 减少了有在 24 h 的 669.5-269.45 nmol/L 的 IC 50 价值的 TM4 Sertoli 房间的生存能力。TP (125, 250,和 500 nmol/L ) dose-dependently 在 TM4 房间增加了 ROS, JNK 的 phosphorylation, mitochondrial 机能障碍和内在的 apoptosis 小径的激活的累积。这些过程被合作处理与抗氧化剂 N 乙酰半胱氨酸稀释(NAC, 1 mmol/L ) 。而且, TP 处理从细胞质禁止了 Nrf2 的 translocation 进原子核以及下游的基因 NAD (P) H quinone 的表示 oxidoreductase1 (NQO1 ) ,过氧化氢酶(猫) 和 hemeoxygenase 1 (HO-1 ) ,因此对氧化应力废除调停 Nrf2 的防卫机制。而且, Nrf2 击倒的 siRNA 显著地加强了 TM4 房间的导致 TP 的 apoptosis。从在 vitro 实验的上述结果进一步在 TP 的口头的管理以后在雄的老鼠被验证(30, 60,和 120 mg 吗? | Yu WANG Su-han GUO Xue-jun SHANG Li-sha YU Jian-wei ZHU Ang ZHAO Yan-fen ZHOU Guo-hua AN Qi ZHANG BO MA | 2018 | Acta Pharmacologica Sinica2018，39，2： | 22 |
| 9 | Relationship between tumor necrosis factor-α and liver fibrosis显示文摘RelationshipbetweentumornecrosisfactorαandliverfibrosisWANGXin,CHENYueXiang,XUCaiFu,ZHAOGuoNing,HUANGYuXinandWANGQinLiD... | WANG Xin, CHEN Yue Xiang, XU Cai Fu, ZHAO Guo Ning, HUANG Yu Xin and WANG Qin Li Department of Gastroenterology, Tangdu Hospital, The Fourth Military Medical University, Xi′an 710038, Shaanxi Province, China | 1998 | World Journal of Gastroenterology1998，4，1： | 21 |
| 10 | Pollen-inferred vegetation and environmental changes since 16.7 ka BP at Balikun Lake, Xinjiang显示文摘A high-resolution fossil pollen record from the sedimentary cores of Balikun Lake, northwestern China, combined with modern surface pollen data, is used to reconstruct the history of vegetation and climatic change since 16.7 cal. ka BP. Fossil pollen assem-blages and lithology indicate that the study area was dominated by desert. The desert had extremely arid climate and lower effec-tive moisture during 16.7–7.9 cal. ka BP, especially from 16.7 to 8.9 cal. ka BP when the lake maybe dried up. During 8.9–7.9 cal. ka BP, the environment gradually recovered in this area. It was then followed by the optimum period from 7.9 to 4.3 cal. Ka BP, when the effective moisture obviously increased. It was characterized by the typical desert-steppe/steppe vegetation and was ac-companied with several patch-birch woodlands around the lake. After that, a short but extremely arid climatic event occurred during 4.3–3.8 cal. ka BP, and the vegetation quickly changed from desert-steppe/steppe to desert. It was a relatively optimum period from 3.8 to 0.53 cal. ka BP showing typical desert-steppe/meadow-steppe landscape. Since 0.53 cal. ka BP, the climate has shown signs of deteriorating again. Furthermore, regional comparison shows that the characteristics of climatic and environmental evolution in this area were clearly different from East Asia monsoonal area during the last 16.7 cal. ka BP. It was characterized by the arid climate during the late-glacial and early Holocene, and relatively wet during the mid-late Holocene. | TAO ShiChen AN ChenBang CHEN FaHu TANG LingYu WANG ZongLi LU YanBin LI ZhiFei ZHENG TongMing ZHAO JiaJu | 2010 | Chinese Science Bulletin2010，55，22： | 20 |
| 11 | Precise nanomedicine for intelligent therapy of cancer显示文摘Precise nanomedicine has been extensively explored for efficient cancer imaging and targeted cancer therapy, as evidenced by a few breakthroughs in their preclinical and clinical explorations. Here, we demonstrate the recent advances of intelligent cancer nanomedicine, and discuss the comprehensive understanding of their structure-function relationship for smart and efficient cancer nanomedicine including various imaging and therapeutic applications, as well as nanotoxicity. In particular, a few emerging strategies that have advanced cancer nanomedicine are also highlighted as the emerging focus such as tumor imprisonment, supramolecular chemotherapy, and DNA nanorobot. The challenge and outlook of some scientific and engineering issues are also discussed in future development. We wish to highlight these new progress of precise nanomedicine with the ultimate goal to inspire more successful explorations of intelligent nanoparticles for future clinical translations. | Huabing Chen Zhanjun Gu Hongwei An Chunying Chen Jie Chen Ran Cui Siqin Chen Weihai Chen Xuesi Chen Xiaoyuan Chen Zhuo Chen Baoquan Ding Qian Dong Qin Fan Ting Fu Dayong Hou Qiao Jiang Hengte Ke Xiqun Jiang Gang Liu Suping Li Tianyu Li Zhuang Liu Guangjun Nie Muhammad Ovais Daiwen Pang Nasha Qiu Youqing Shen Huayu Tian Chao Wang Hao Wang Ziqi Wang Huaping Xu Jiang-Fei Xu Xiangliang Yang Shuang Zhu Xianchuang Zheng Xianzheng Zhang Yanbing Zhao Weihong Tan Xi Zhang Yuliang Zhao | 2018 | Science China Chemistry2018，61，12： | 19 |
| 12 | Numerical modeling and dynamic analysis of the 2017 Xinmo landslide in Maoxian County, China显示文摘A catastrophic landslide occurred at Xinmo village in Maoxian County, Sichuan Province,China, on June 24, 2017. A 2.87×106 m3 rock mass collapsed and entrained the surface soil layer along the landslide path. Eighty-three people were killed or went missing and more than 103 houses were destroyed. In this paper, the geological conditions of the landslide are analyzed via field investigation and high-resolution imagery. The dynamic process and runout characteristics of the landslide are numerically analyzed using a depth-integrated continuum method and Mac Cormack-TVD finite difference algorithm.Computational results show that the evaluated area of the danger zone matchs well with the results of field investigation. It is worth noting that soil sprayed by the high-speed blast needs to be taken into account for such kind of large high-locality landslide. The maximum velocity is about 55 m/s, which is consistent with most cases. In addition, the potential danger zone of an unstable block is evaluated. The potential risk area evaluated by the efficient depthintegrated continuum method could play a significant role in disaster prevention and secondary hazard avoidance during rescue operations. | OUYANG Chao-jun ZHAO Wei HE Si-ming WANG Dong-po ZHOU Shu AN Hui-cong WANG Zhong-wen CHENG Duo-xiang | 2017 | Journal of Mountain Science2017，14，9： | 19 |
| 13 | Clinical outcomes of EGFR-TKI treatment and genetic heterogeneity in lung adenocarcinoma patients with EGFR mutations on exons 19 and 21显示文摘Background:Epidermal growth factor receptor(EGFR) mutations,including a known exon 19 deletion(19 del) and exon 21 L858 R point mutation(L858R mutation),are strong predictors of the response to EGFR tyrosine kinase inhibitor(EGFR-TKI) treatment in lung adenocarcinoma.However,whether patients carrying EGFR 19 del and L858 R mutations exhibit different responsiveness to EGFR-TKls and what are the potential mechanism for this difference remain controversial.This study aimed to investigate the clinical outcomes of EGFR-TKI treatment in patients with EGFR 19 del and L858 R mutations and explore the genetic heterogeneity of tumors with the two mutation subtypes.Methods:Of 1127 patients with advanced lung adenocarcinoma harboring EGFR 19 del or L858 R mutations,532 received EGFR-TKI treatment and were included in this study.EGFR 19 del and L858 R mutations were detected by using denaturing high-performance liquid chromatography(DHPLC).T790 M mutation,which is a common resistant mutation on exon 20 of EGFR,was detected by amplification refractory mutation system(ARMS).Next-generation sequencing(NGS) was used to explore the genetic heterogeneity of tumors with EGFR 19 del and L858 R mutations.Results:Of the 532 patients,319(60.0%) had EGFR 19 del,and 213(40.0%) had L858 R mutations.The patients with EGFR 19 del presented a significantly higher overall response rate(ORR) for EGFR-TKI treatment(55.2%vs.43.7%,P = 0.017) and had a longer progression-free survival(PFS) after first-line EGFR-TKI treatment(14.4 vs.11.4 months,P = 0.034) compared with those with L858 R mutations.However,no statistically significant difference in overall survival(OS) was observed between the two groups of patients.T790 M mutation status was analyzed in 88 patients before EGFR-TKI treatment and 134 after EGFR-TKI treatment,and there was no significant difference in the co-existence of T790 M mutation with EGFR 19 del and L858 R mutations before EGFR-TKI treatment(5.6%vs.8.8%,P = 0.554)or after treatment(24.4%vs.35.4%,P = 0.176).In addition,24 patients with EGFR 19 del and 19 with L858 R mutations were analyzed by NGS,and no significant difference in the presence of multiple somatic mutations was observed between the two genotypes.Conclusions:Patients with EGFR 19 del exhibit longer PFS and higher ORR compared with those with L858 R mutations.Whether the heterogeneity of tumors with EGFR 19 del and L858 R mutations contribute to a therapeutic response difference needs further investigation. | Jiang-Yong Yu Si-Fan Yu Shu-Hang Wang Hua Bai Jun Zhao Tong-Tong An Jian-Chun Duan Jie Wang | 2016 | Chinese Journal of Cancer2016，35，4： | 19 |
| 14 | Effect of L-NAME on nitric oxide and gastrointestinal motility alterations in cirrhotic rats显示文摘AIM: To invsstigare the effect of L-NAME on nitric oxide andgastriubtestubal motility alterations in cirrhotic ratsMETHODS: Rats with cirrhosis induced by carbontetrachloride were randomly divided into two groups, one( n= 13) receiving 0. 5 mg@ kg-1 per clay of NG-nitro-L-argininemethyl ester (L-NAME), a nitric oxide synthase inhibitor,for 10 days, whereas the other group ( n = 13) and control( n = 10) rats were administrated the same volume of 9 g@ L-1saline.Half gastric emptying time and 2 h residual rate weremeasured by SPECT, using 99m Tc-DTPA-labeled bariumsuifate as test meal. Gastrointestinal transition time wasrecorded simultaneously. Serum concentration of nitrcoxide (NO) was determined by the kinetic cadmiunreduction and colorimetric methods. ImmunohistochemicalSABC method was used to observe the expression anddistribution of three types of nitric oxide synthase (NOS)isoforms in the mt gastrointestinal tract. Western blot wasused to detect expression of gastrointestinal NOS isoforms.RESULTS: Half gastric emptying time and trans-gastrointestinal time were significantly prolonged( 124.0 ± 26.4min; 33.7± 8.9min;72.1 ± 15.3 min; P<0.01), (12.4±0.5h; 9.5±0.3 h; 8.2±0.8 h; P<0.01), 2h residual rate wasraised in cirrhotic rots than in controls and cirrhotic ratstreated with L-NAME(54.9± 7.6 % ,13.7 ± 3.2 %, 34.9± 10.3%, P< 0.01). Serum concentration of NO was significantlyincreased in cirrhotic rots than in the other groups (8.20 ± 2.48)μmol@L-1, (5.94± 1.07) μmol@L-1 ,and control (5.66± 1.60) tμmol@L-1, P< 0.01. NOS staining intensities which weremainly located in the gastrointestinal tissues were markedlylower in cirrhotic rats than in the controls and cirrhotic ratsafter treated with L- NAME.CONCLUSION: Gastrointestinal motility was remarkablyinhibited in cirrhotic rats, which could he alleviated by L-NAME. Nitric oxide may play an important role in theinhibition of gastrointestinal motility in cirrhotic rats. | Xin Wang Zong-You Zhang Mei Lan Ji-Yan Miao Xue-Gang Guo Yong-Quan Shi Yan-Qiu Zhao Jie Ding Kai-Cun Wu Dai-Ming Fan,Institute of Digestive disease,Xijing Hospital,Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China Yue-Xia Zhong,Emergency Department,Tangdu Hospital,Fourth Military Medical University,Xi’an 710038,Shaanxi Province,China Ju Lu,Class EE 87,Department of Electronic Engineering,Tsinghua University,Beijing 100084,China Bo-Rong Pan,Oncology Center,Xijing Hospital,Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China | 2002 | World Journal of Gastroenterology2002，8，2： | 19 |
| 15 | THE INFLUENCE OF CAVITY DAMAGE ON MECHANICAL PROPERTIES OF SUPERPLASTICALLY-DEFORMED MATERIALS AND ITS PREDICTION显示文摘Aluminium alloy LY12CZ sheet without any pre-treatment has been used to study the influence of cavity damage on the mechanical properties of the superplastically-deformed materials room temper- ature. The experimental results show that: (1) the lower the rate of cavitation as the superplastic strain increases, the higher the superplasticity is; (2) the mechanical properties of superplastically- deformed materials at room temperature decrease as the superplastically-deformed strain increases, especially, a noteworthy decrease in elasticity modulus, yield strenth and ultimate strength at room temperature begins to appear as the level of cavitation by area comes up to about 4%, while at the same level the reduction in area drops down a very large quantity. Then, a three-layer back- propagation neural network has been developed to predict the machanichl properties of the superplasti- cally-deformed material.The results acquired from the neural network are very | M. B. Liu, Z. C. Zhao,H. Gao,M. Q. Li and S. C. Wu College of Materials Science and Engineering, Northwestern Polytechnical University, Xi’an 710072, China | 2000 | Acta Metallurgica Sinica(English Letters)2000，13，2： | 18 |
| 16 | A fishy odor episode in a north China reservoir: Occurrence, origin, and possible odor causing compounds显示文摘A significant outbreak of fishy odor occurred in a reservoir located in Inner Mongolia, China, in the winter of 2011, and the odor rating, algal density and concentrations of some potential odorous compounds were monitored over a period of two months. The peak odor rating of the fishy odor was 7 according to flavor profile analysis. Among the dominant algal species(two diatom and one chrysophyte species) observed during the survey, the chrysophyte Dinobryon sp. was the most abundant species, with the peak density recorded at 88,520 cells/mL. Seven potential algal metabolites including heptanal, 2,4-heptadienal, 2,4-decadienal, nonanal, 2-octenal, 2,6- nonadienal and hexanal were detected. The principal component analysis result showed that n-hexanal, n-heptanal and 2,4-decadienal, possibly the metabolites of diatoms, and 2,4-heptadienal, possibly the metabolite of Dinobryon sp., might have contributed to the fishy odor episode. This study demonstrated that the fishy odor episode in this reservoir might be caused by the abnormal growth of chrysophytes and diatoms under the ice-cover. | Yunyun Zhao Jianwei Yu Ming Su Wei An Min Yang | 2013 | Journal of Environmental Sciences2013，25，12： | 17 |
| 17 | Effects of chemical fertilizer combined with organic manure on Fuji apple quality,yield and soil fertility in apple orchard on the Loess Plateau of China显示文摘To evaluate the effects of chemical fertilizer combined with organic manure on apple yield,quality and soil fertility,an experiment was conducted in an apple orchard on the Loess Plateau of China.Six treatments,i.e.,1)no nitrogen(N)with chemical phosphorus(P)and potassium(K)(PK),2)no P with chemical N and K(NK),3)no K with chemical N and P(NP),4)N,P and K chemical fertilizers only(NPK),5)swine manure(M)only(M),and 6)half chemical fertilizers combined with half swine manure(NPKM)were included with three replications for each.The NPKM treatment achieved 36.9 t/ha average annual yield,which was 42.5%greater than the yield of PK treatment.The average annual yields followed the sequence of NPKM>NPK>M>NK>NP>PK.In NPKM treatment 71.3%of the collected apples had an apple diameter greater than 80 mm compared with 58.2%,41.5%and 37.2%in NK,PK and NP treatments,respectively.The sugar to acid(S:A)ratio was the greatest in NPKM treatment.The results of Vitamin C,soluble solid and firmness showed that NPKM treatment had the highest values.The concentration of soil organic carbon(SOC)in the 0 to 20 cm depth of soil was significantly affected by addition of M.Compared to the antecedent soil properties,the SOCS in the NPKM and M treatments were increased by 28.8%,29.4%,and TN contents were 56.5,49.8%more for soil at 0-20 cm depths,respectively.The major soil nutrients of N,P and K were also significantly increased by M and NPKM treatments in surface soil for five years.The data support the conclusion that,for a production of 35-40 t/ha in an apple orchard on the Loess Plateau of China,the 25-30 t/ha organic manure,160-200 kg/ha N,100-150 kg/ha P2O5 and 120-160 kg/ha K2O were the most suitable fertilizer application.The finding will be helpful for harmonious development of apple production technology,economic income increase for farmers,and improvement of the apple orchard ecosystem. | Zhao Zuoping Yan Sha Liu Fen Ji Puhui Wang Xiaoying Tong Yan’an | 2014 | International Journal of Agricultural and Biological Engineering2014，7，2： | 17 |
| 18 | Downregulation of miR-130a contributes to cisplatin resistance in ovarian cancer cells by targeting X-linked inhibitor of apoptosis (XIAP) directly显示文摘MicroRNAs (miRNAs ) 是短的,高度保存了小非编码的 RNA 分子,它 post-transcriptionally 调整基因表示并且在多样的生物进程起关键作用。最近的研究证明了 miRNAs 的 dysregulation 可能调制癌症房间的电阻到化学疗法的代理人。为了在人的卵巢的癌症房间在 cisplatin 电阻的发展调查 miR-130a 的可能的角色,衬里 A2780,我们由量的即时反向的抄写聚合酶链反应在房间计算了 microRNA-130a (miR-130a ) 的表达式。结果证明 miR-130a 在 cisplatin 抵抗的卵巢的癌症房间是显著地下面调整的。MTT 试金和流动 cytometry (FCM ) 结果证明 miR-130a 的在表示上调整了 apoptotic 活动,并且从而 cisplatin chemosensitivity,在卵巢的癌症房间。而且,我们发现 miR-130a 能直接指向 XIAP,并且参予 apoptosis 的规定。miR-130a 的起来规定在 XIAP mRNA 层次和蛋白质层次导致了重要减少。XIAP 在卵巢的癌症房间线 A2780 在 cisplatin 抵抗起一个重要作用。我们的调查结果建议 miR-130a 能在卵巢的癌症房间线 A2780 在 cisplatin 抵抗的发展起一个作用,至少部分地由经由指向 XIAP 的 apoptosis 的调整。 | Xi'an Zhang Lingna Huang Yinglin Zhao Wuhong Tan | 2013 | Acta Biochimica et Biophysica Sinica2013，45，12： | 16 |
| 19 | Rapid and high throughput detection of HBV YMDD mutants with fluorescence polarization显示文摘AIM: To develop a simple and rapid detection of HBV gene variants and prediction of lamivudine-resistance in patients.METHODS: Initially, plasmids harboring the wild-type or mutant HBV DNA fragments were used in a model system.The technique was then applied to clinical samples for an analysis of YMDD mutations. The sera were extracted from chronic hepatitis patients who had received lamivudine treatment for more than one year. P region gene of HBV was amplified by polymerase chain reaction. The excess primers and dNTPs in PCR products were removed by cleaning-up reagents. Template-directed dye-terminator incorporation reaction was performed and R110 or TAMRA labeled acyclo-terminator was added on the 3' end of TDIprimer specifically. Fluorescence polarization value was measured with Victor 2 multilabel counter and the genotypes of HBV were analyzed.RESULTS: The YMDD genotypes in recombined positive plasmid and 56 serum samples of HBV infected patients were analyzed by using our TDI-FP method and the specificity and sensitivity were confirmed by DNA sequencing. Five of 56 serum samples showed YVDD phenotype (9%), including 1 YMDD and YVDD mixed infection. Four of 56 showed YIDD phenotype (7.1%).CONCLUSION: This is a simple, rapid, low cost and high throughput assay to detect HBV polymerase gene variants and suitable for large-scale screening and prediction of the lamivudine-resistance in clinical samples. | Yui-Jie Bai Jin-Rong Zhao Guan-Ting Lv Wen-Hong Zhang Yan Wang Xiao-Jun Yan, Institute of Genetic Diagnosis, Fourth Military Medical University, Xi’an 710032, Shannxi Province, China | 2003 | World Journal of Gastroenterology2003，9，10： | 16 |
| 20 | Supramolecular complex formation of β-cyclodextrin polymer with substituted salicylic acid or 3-hydroxy-2-naphthoic acid and their electrorheological behaviors显示文摘According to the chemical design, electrorheological properties of supramolecular complex from b-cyclodextrin polymer (b -CDP) were discussed. Six supramolecular complexes of -cyclodextrin polymer with substituted salicylic acid and 3-hydroxy-2-naphthoic acid were syn-thesized by the solid-phase self-assembly method, and their component and structure were characterized by NMR, FT-IR, UV-vis and the fluorescence analysis. Then the electrorheological properties of their suspensions in silicone oil were investigated under DC electric fields. It was found that the yield stresses of these supramolecular complex ER fluids were 7.3—9.8 kPa at 4 kV/mm in DC electric field, which were enhanced by 34%—72% compared with that of pure b-CDP. Among them, that of b-CDP/3-hydroxy-2-naphthoic acid ER fluid was the highest. It was also found that the ER effect of supramolecular complexes can be controlled by changing dif-ferent guests. When the substituted group is at phenyl ring, ER behavior can be slightly adjusted by the different substituted groups, their number as well as their position at phenyl ring. This can be proved by the measurement of dielectric properties. | GAO Ziwei1,2, ZHAO Xiaopeng1, SUN Ping2 & SI Gang2 1. Institute of Electrorheological Technology141#, Department of Applied Physics, Northwestern Polytechnical University, Xian 710072, China 2. Department of Chemistry, School of Chemistry and Material Science, Shaanxi Normal University, Xi’an 710062, China | 2004 | Science China Chemistry2004，47，4： | 15 |
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