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| 1 | Depletion of activated hepatic stellate cell correlates with severe liver damage and abnormal liver regeneration in acetaminophen-induced liver injury显示文摘肝的星形的房间(HSC ) 是为肝的祖先房间(HPC ) 和 hepatocytes 的本地干细胞壁龛的重要部分。然而,它至于激活的 HSC 的产品是否被要求稀释 hepatocyte 损害是不清楚的,提高肝新生,或两个。在这研究,我们由弄空执行了功能研究的损失有 gliotoxin 的激活的 HSC。显著地严重的肝损坏和拒绝的幸存率与激活的 HSC 的弄空被相关,这被表明。而且,减少 HSC 激活导致了增殖的 hepatocytes 的数字的 hepatocyte apoptosis 和 66% 减少的 3 褶层增加。这被戏剧的减少在知道在 hepatocyte 复制期间起来调整的五基因的表示层次伴随。特别地,激活的 HSC 的弄空禁止了被 Pank 积极的房间的减少的数字在道和降低的基因表示 cytokeratin 铺平的门附近证实的卵形的房间反应,这被发现 19 (CK19 ) 在对待 gliotoxin 的肝。这些数据提供激活的 HSC 涉及 hepatocyte 死亡和 hepatocytes 的增长,在 acetaminophen (APAP ) 的 HPC 导致了尖锐的肝损害的清楚的证据。 | Kuntang Shen Wenju Chang Xiaodong Gao Hongshan Wang Weixin Niu Lujun Song Xinyu Qin | 2011 | Acta Biochimica et Biophysica Sinica2011，43，4： | 13 |
| 2 | Synergistic effect of ozonation and ionizing radiation for PVA decomposition显示文摘Ozonation and ionizing radiation are both advanced oxidation processes(AOPs) without chemical addition and secondary pollution. Also, the two processes' efficiency is determined by different p H conditions, which creates more possibilities for their combination. Importantly,the combined process of ozonation and ionizing radiation could be suitable for treating wastewaters with extreme p H values, i.e., textile wastewater. To find synergistic effects, the combined process of ozonation and ionizing radiation mineralization was investigated for degradation of polyvinyl alcohol(PVA) at different p H levels. A synergistic effect was found at initial p H in the range 3.0–9.4. When the initial p H was 3.0, the combined process of ozonation and ionizing radiation gave a PVA mineralization degree of 17%. This was 2.7 times the sum achieved by the two individual processes, and factors of 2.1 and 1.7 were achieved at initial p H of7.0 and 9.4, respectively. The combined process of ozonation and ionizing radiation was demonstrated to be a feasible strategy for treatment of PVA-containing wastewater. | Weihua Sun Lujun Chen Yongming Zhang Jianlong Wang | 2015 | Journal of Environmental Sciences2015，27，8： | 7 |
| 3 | Isolation and culture of hepatic stellate cells from mouse liver显示文摘肝的星形的房间(HSC ) 是在肝以内的主要细胞外的生产矩阵的房间并且有众多的 vitalfunctions。为 HSC 的隔离和文化的一个柔韧的协议是重要的因为房间 functionsand 的进一步的调查在肝疾病联系了机制。鼠标肝的卷比老鼠肝的小得多,哪个对更困难的 makesit 比老鼠 HSC 孤立鼠标 HSC (mHSCs ) 。目前,孤立 mHSCs 仍然是挑战 becausethere 不是有效、柔韧的方法孤立并且文化这些房间。在现在的学习, C57BL/6J 老鼠是有包含 liposome 的 dichloromethylene diphosphate (CL2MDP ) 的 intravenouslyinjected 有选择地从 theliver 消除 Kupffer 房间。老鼠肝然后在 situ 被酒,并且 mHSCs 与一种优化密度坡度 centrifugation 技术被孤立。在磷酸盐缓冲区答案(PBS )-liposome 组, mHSCs 的收益是(1. | Wenj u Chang Mengxuan Yang Lujun Song Kuntang Shen Hongshan Wang Xiaodong Gao Min Li Weixin Niu Xinyu Qin | 2014 | Acta Biochimica et Biophysica Sinica2014，46，4： | 6 |
| 4 | Protophyllocladoxylon jingyuanense sp.nov.,a Gymnospermous Wood of the Serpukhovian(Late Mississippian) from Gansu,Northwest China显示文摘裸子植物的石块木头的新种, Protophyllocladoxylon jingyuanense sp。nov,从 Tsingyuan 形成的更低的部分被描述(Serpukhovian/Late 密西西比河) 在 Ciyao 的煤矿附近, Gansu,西北的中国。当生长戒指是在光线的 tracheidal 墙中的不在的坑时,标本仅仅在硅化的第二等的木部被保存是在类型的 araucaroid, uniseriate,偶尔 biseriate。木部光线是同类的, uniseriate,偶尔 biseriate。跨地的坑简单,大,一~二在里面数字。轴的实质是不在的。解剖人物和整个地质的年龄的 Protophyllocladoxylon 森林的地理分发被记录。有轴的实质并且没有生长的那些种类在温暖的气候现场响,而没有轴的实质并且与生长戒指的种类也在温暖或凉爽的气候是在场的。为 Protophyllocladoxylon 森林的理想的生活气候温暖、湿。我们的新种类一根石块木头早含碳,是可能的 Protophyllocladoxylon 的最早已知的种类。 | ZHANG Yi WANG Jun LIU Lujun LI Nan | 2010 | Acta Geologica Sinica(English Edition)2010，84，2： | 5 |
| 5 | Evolution of microstructure and high temperature tensile properties of as-extruded Ti Bw reinforced near-α titanium matrix composite subjected to heat treatments显示文摘The Ti Bw reinforced near-α titanium matrix composite(Ti-5.8 Al-3.4 Zr-4.0 Sn-0.4 Mo-0.4 Nb-0.4 Si-0.06 C) was successfully synthesized by powder metallurgy and hot extrusion route. The effects of solution and aging temperature on the microstructure and high temperature tensile properties of the composite were investigated. The results revealed that the fine transformed β phase can be obtained by the solution treatment at β phase region and aging treatment, no other precipitates were observed. The α2 phase(Ti3 Al) can be acquired when the solution treated at α+β phase region followed by aging treatment. With increasing the aging temperature from 500 to 700°C for 5 h, the size of α2 precipitates increases from about 5 to about 30 nm. The Ti Bw are stable without any interfacial reaction during the heat treatments. The high temperature tensile properties show that the composite performed by solution and aging treatment exhibits good strengthening effects. With increasing the aging temperature from 500 to 700°C, the strength of the composite increases at the expense of elongation due to the increment of α2 precipitates.The strength of the composite at 600°C increases by 17% to 986 MPa after 1000°C/2 h/AC and 700°C/5 h/AC heat treatment. | WANG Bo ZHANG HaoBiao HUANG LuJun HE JianLi ZHANG YouFeng WANG Wei | 2018 | Science China(Technological Sciences)2018，61，9： | 5 |
| 6 | Structural Engineering of Hierarchical Aerogels Comprised of Multi-dimensional Gradient Carbon Nanoarchitectures for Highly Efficient Microwave Absorption显示文摘Recently,multilevel structural carbon aerogels are deemed as attractive candidates for microwave absorbing materials.Nevertheless,excessive stack and agglomeration for low-dimension carbon nanomaterials inducing impedance mismatch are significant challenges.Herein,the delicate“3D helix-2D sheet-1D fiber-0D dot”hierarchical aerogels have been successfully synthesized,for the first time,by sequential processes of hydrothermal self-assembly and in-situ chemical vapor deposition method.Particularly,the graphene sheets are uniformly intercalated by 3D helical carbon nanocoils,which give a feasible solution to the mentioned problem and endows the as-obtained aerogel with abundant porous structures and better dielectric properties.Moreover,by adjusting the content of 0D core-shell structured particles and the parameters for growth of the 1D carbon nanofibers,tunable electromagnetic properties and excellent impedance matching are achieved,which plays a vital role in the microwave absorption performance.As expected,the optimized aerogels harvest excellent performance,including broad effective bandwidth and strong reflection loss at low filling ratio and thin thickness.This work gives valuable guidance and inspiration for the design of hierarchical materials comprised of dimensional gradient structures,which holds great application potential for electromagnetic wave attenuation. | Yongpeng Zhao Xueqing Zuo Yuan Guo Hui Huang Hao Zhang Ting Wang Ningxuan Wen Huan Chen Tianze Cong Javid Muhammad Xuan Yang Xinnan Wang Zeng Fan Lujun Pan | 2021 | Nano-Micro Letters2021，13，9： | 5 |
| 7 | NS1-binding protein radiosensitizes esophageal squamous cell carcinoma by transcriptionally suppressing c-Myc显示文摘Background:Cisplatin-based chemotherapy with concurrent radiotherapy is a standard treatment for advanced esophageal squamous cell carcinoma(ESCC).NS1-binding protein(NS1-BP),a member of the BTB-kelch protein fam-ily,has been shown to inhibit the proliferation of Hela cells by suppressing c-Myc.In the present study,we examined the potential function role of NS1-BP expression in ESCC,and particularly,the sensitivity of ESCC to radiotherapy.Methods:NS1-BP expression was examined using immunohistochemistry in two cohorts(n=98 for the training cohort;n=46 for independent validation cohort)of ESCC patients receiving cisplatin-based chemotherapy and con-current radiotherapy.Normal esophageal mucosal tissue blocks were used as a control.We also conducted a series of in vitro and in vivo experiments to examine the potential effects of over-expressing NS1-BP on ESCC cells,and particu-larly their sensitivity to ionizing irradiation.Results:In the training cohort,NS1-BP downregulation was observed in 59%(85/144)of the ESCC specimens.NS1-BP downregulation was associated with chemoradiotherapeutic resistance and shorter disease-specific survival(DSS)in both the training and validation cohorts.Over-expressing NS1-BP in cultured ESCC cells substantially increased the cellular response to irradiation both in vitro and in vivo.NS1-BP also significantly enhanced IR-induced apoptosis,and abrogated IR-induced G2/M cell-cycle arrest and ATM/Chk1 phosphorylation.Immunoprecipitation assays indicated that NS1-BP could interact with c-Myc promoter regions to inhibit its transcription.In ESCC tissues,c-Myc expression was inversely correlated with NS1-BP levels,and was associated with a shorter DSS.Conclusions:Our findings highlight the role and importance of NS1-BP in radiosensitivity of ESCC.Targeting the NS1-BP/c-Myc pathway may provide a novel therapeutic strategy for ESCC. | Yuwen Wang Jingjing Cheng Dan Xie Xiaofeng Ding Hailing Hou Xi Chen Puchun Er Furong Zhang Lujun Zhao Zhiyong Yuan Qingsong Pang Ping Wang Dong Qian | 2018 | Cancer Communications2018，38，1： | 4 |
| 8 | Radiation induced decomposition of a refractory cefathiamidine intermediate显示文摘Diisopropylthiourea(DPT),an intermediate of a widely used cephalosporin,has been found to be one of the most refractory components in cephalosporin synthesis wastewater.This compound cannot be completely removed by conventional biological processes due to its antimicrobial property.Ionizing radiation has been applied in the decomposition of refractory pollutants in recent years and has proved effective.Therefore,the decomposition of DPT byγ-irradiation was studied.The compound was irradiated at the dose of 150–2000 Gy before a change of concentration and UV absorption of the solutions was detected.Furthermore,the decomposition kinetics and radiation yield(G-value)of DPT was investigated.The results of radiation experiments on DPT-containing aqueous showed that the DPT can be effectively degraded byγ-radiation.DPT concentration decreased with increasing absorbed doses.G-values of radiolytic decomposition for DPT(20 mg/L)were 1.04and 0.47 for absorbed doses of 150 and 2000 Gy,respectively.The initial concentration and p H of the solutions affected the degradation.As the concentration of substrate increased,the decomposition was reduced.The decrease of removal rate and radiation efficacy under alkaline condition suggested that lower p H values benefit theγ-induced degradation.UV absorption from 190 to 250 nm decreased after radiation while that from 250 to 300 nm increased,indicating the formation of by-products. | Qiburi Bao Lujun Chen Jianlong Wang | 2014 | Journal of Environmental Sciences2014，26，12： | 4 |
| 9 | Spectrofluorimetric determination of zinc(Ⅱ)with N-salicylidene phenylalanine显示文摘Spectrofluorimetricdeterminationofzinc(Ⅱ)withN-salicylidenephenylalanineLiuShaopu;WangLujun;ZhouGuangming(InstttvteojEnvironm... | Liu Shaopu Wang Lujun Zhou Guangming(Instttvte oj Environmental Chemistry, Souxthwest Mormal University,Chongqing 630715) | 1995 | 西南师范大学学报(自然科学版)1995，20，4： | 3 |
| 10 | Growth of Carbon Nanocoils by Porous α-Fe2O3/SnO2 Catalyst and Its Buckypaper for High Efficient Adsorption显示文摘High-purity(99%)carbon nanocoils(CNCs)have been synthesized by using porousα-Fe2O3/SnO2 catalyst.The yield of CNCs reaches 9,098%after a 6 h growth.This value is much higher than the previously reported data,indicating that this method is promising to synthesize high-purity CNCs on a large scale.It is considered that an appropriate proportion of Fe and Sn,proper particle size distribution,and a loose-porous aggregate structure of the catalyst are the key points to the high-purity growth of CNCs.Benefiting from the high-purity preparation,a CNC Buckypaper was successfully prepared and the electrical,mechanical,and electrochemical properties were investigated comprehensively.Furthermore,as one of the practical applications,the CNC Buckypaper was successfully utilized as an efficient adsorbent for the removal of methylene blue dye from wastewater with an adsorption efficiency of 90.9%.This study provides a facile and economical route for preparing high-purity CNCs,which is suitable for large-quantity production.Furthermore,the fabrication of macroscopic CNC Buckypaper provides promising alternative of adsorbent or other practical applications. | Yongpeng Zhao Jianzhen Wang Hui Huang Tianze Cong Shuaitao Yang Huan Chen Jiaqi Qin Muhammad Usman Zeng Fan Lujun Pan | 2020 | Nano-Micro Letters2020，12，2： | 3 |
| 11 | Proliferation and differentiation potential of mouse adult hepatic progenitor cells cultured in vitro显示文摘,这研究试图孤立干细胞或祖先存在,从正常成年老鼠肝并且调查他们增长和区别的潜力。Hepatocytes 被修改二拍子的圆舞肝灌注方法和 centrifugation 孤立,然后在为观察的修改包含浆液的 DMEM 有教养超过 60 天。Immunofluorescence 技术被使用检查 hepatocytes 并且与白朊, -fetoprotein (法新社) 和 cytokeratin 检验殖民地的形成 19 (CK19 ) 。结果显示出为 hepatocyte 是强烈积极的那一些 hepatocytes 在文化的白天 1 上的特定的标记白朊,能在天被激活 23,由殖民地的快速的增长和形成列在后面。殖民地能不断地膨胀超过 60 天。在白天 5 上,在殖民地的所有房间表示了肝的干细胞(HSC ) 标记法新社。与文化的时间,在殖民地的一些房间失去了能力在天划分 1315,并且区分了进有大细胞质和大约二个原子核的房间,类似于成熟 hepatocytes 的外观词法上。这些区分的房间表明了白朊的强壮的表示。在白天 30 附近,一些大房间在殖民地出现了并且表示了胆汁管房间标记 CK19。因此,老鼠 hepatocytes 的这 subpopulation 能获得不成熟的 hepatocytes 的一些特征并且与区别的一个高增殖能力和双性人潜力显示出肝的祖先房间的侧面。他们从因此,成年人老鼠说出成年肝的祖先房间(AHPC ) 的正常被孤立。老鼠 AHPC 可以为 hepatocytes 移植和 hepatopathy 学习被用作一个 HSC 模型。 | Lujun Song Hongshan Wang Xiaodong Gao Kuntang Shen Weixin Niu Xinyu Qin | 2010 | Acta Biochimica et Biophysica Sinica2010，42，2： | 3 |
| 12 | Gamma irradiation of 2-mercaptobenzothiazole aqueous solution in the presence of persulfate显示文摘Recently, water treatment by ionizing radiation has gained increasing attention as a powerful technology for the destruction of refractory pollutants. 2-Mercaptobenzothiazole(MBT) is known as a widespread, toxic and poorly biodegradable pollutant. This paper studied the gamma irradiation of aqueous solutions of MBT. Moreover, the effect of the addition of persulfate(S2O82-) on the radiolytic destruction of MBT was investigated. The main transformation products of the studied compound were detected and the sequence of occurrence of the products was described. The change of biodegradability of MBT solution was also observed. The main results obtained in this study indicated that gamma radiation was effective for removing MBT in aqueous solution. Persulfate addition, which induced the formation of reactive sulfate radicals(SO4-U), greatly enhanced the degradation of MBT. Benzothiazole was identified as the first radiation product, followed by 2-hydroxybenzothiazole. Decomposition of MBT started with the oxidation of –SH groups to sulfate ions. Possible pathways for MBT decomposition by gamma irradiation were proposed. The BOD/COD ratios of MBT samples were increased after radiation,indicating the improvement of biodegradability and reduction of toxicity. | Qiburi Bao Lujun Chen Jinping Tian Jianlong Wang | 2016 | Journal of Environmental Sciences2016，28，8： | 3 |
| 13 | Constitutive equation and hot deformation characteristic of Ti-6.5Al-3.5Mo-1.5Zr-0.3Si alloy with equiaxed microstructure显示文摘The hot deformation behavior of Ti-6.5Al-3.5Mo-1.5Zr-0.3Si alloy with equiaxed microstructure was characterized in the temperature range of 900~1060 ℃ and strain rate range of 10-3~10 s-1.The experimental results indicate that the plastic deformation behavior of the titanium alloys is rather sensitive to temperature and strain rate.In the α+β phase temperature region,all of the stress-strain curves exhibit different degrees of the flow softening after a peak stress.In the β phase temperature region,the titanium alloy shows a stress softening at high strain rates and a steady flow stress at low strain rates.On the basis of the peak stress data,the constitutive equations were constructed in the α+β phase temperature region and β phase temperature region,respectively.Activation energy parameters were calculated to be 344.923 kJ·mol-1 in the β phase temperature region and 628.3 kJ·mol-1 in the α+β phase temperature region.Microstructure of the compressed specimens in water-quenched conditions was found to be quite dependent on the conditions of deformation. | HUANG Lujun,GENG Lin,LI Aibin,WANG Guisong,and SHI Lei School of Materials Science and Engineering,Harbin Institute of Technology,Harbin 150001,China | 2007 | Rare Metals2007，26，S1： | 3 |
| 14 | NS1结合蛋白通过转录抑制c-Myc增强食管鳞状细胞癌的放疗敏感性显示文摘背景与目的基于顺铂的化疗联合放疗是晚期食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)的标准疗法。NS1结合蛋白(NS1-binding protein,NS1-BP)是BTB-kelch蛋白家族中的一员,已被证明可通过抑制c-Myc进而抑制HeLa细胞增殖。在本研究中,我们检测NS1-BP表达在ESCC中的潜在作用,特别是其在ESCC放疗敏感性方面的作用。方法对于两组接受基于顺铂化疗且联合放疗的ESCC患者(研究组n=98;验证组n=46),采用免疫组化方法分别检测NS1-BP表达。使用正常食管黏膜组织块作为对照。我们也进行了一系列体外和体内实验来检测过表达NS1-BP对ESCC细胞各方面的潜在影响,尤其关注的是对电离辐射的敏感性方面的影响。结果在研究组中,59%(85/144)的ESCC样本观察到了NS1-BP下调。在研究组和验证组中,NS1-BP下调与放化疗抵抗和较短的疾病特异生存期(disease-specific survival,DSS)均具有相关性。体内和体外培养条件下过表达NS1-BP均能大幅度提高ECSS细胞对放疗的敏感性。NS1-BP也显著增强IR诱导的凋亡,阻断IR诱导的G2/M细胞周期阻滞和ATM/Chk1磷酸化。免疫沉淀反应显示NS1-BP可以与c-Myc启动子区相互作用从而抑制其转录。在ESCC组织中,c-Myc表达与NS1-BP水平负相关,与较短的疾病特异生存期相关。结论我们的研究结果显示了NS1-BP在ESCC放疗敏感性中的作用和重要性。以NS1-BP/c-Myc通路为靶标有望开发一种新的ESCC治疗策略。 | Yuwen Wang Jingjing Cheng Dan Xie Xiaofeng Ding Hailing Hou Xi Chen Puchun Er Furong Zhang Lujun Zhao Zhiyong Yuan Qingsong Pang Ping Wang Dong Qian | 2019 | 癌症2019，38，2： | 2 |
| 15 | Tissue factor expression in rheumatoid synovium: A potential role in pannus invasion of rheumatoid arthritis显示文摘 | Lujun Chen Yahua Lu Yang Chu Jun Xie Wen’ge Ding Fengming Wang | 2013 | Acta Histochemica2013，，： | 2 |
| 16 | A preliminary study on the characterization of follicular helper T (Tfh) cells in rheumatoid arthritis synovium显示文摘 | Yang Chu Fengming Wang Meng Zhou Lujun Chen Yahua Lu | 2013 | Acta Histochemica2013，，： | 2 |
| 17 | Distributed tasks-platforms scheduling method to holonic-C2 organization显示文摘To solve the problem of distributed tasks-platforms scheduling in holonic command and control(C2) organization,the basic elements of the organization are analyzed firstly and the formal description of organizational elements and structure is provided. Based on the improvement of task execution quality,a single task resource scheduling model is established and the solving method based on the m-best algorithm is proposed. For the problem of tactical decision-holon cannot handle tasks with low priority effectively, a distributed resource scheduling collaboration mechanism based on platform pricing and a platform exchange mechanism based on resource capacities are designed. Finally,a series of experiments are designed to prove the effectiveness of these methods. The results show that the proposed distributed scheduling methods can realize the effective balance of platform resources. | WANG Xun YAO Peiyang ZHANG Jieyong WAN Lujun JIA Fangchao | 2019 | Journal of Systems Engineering and Electronics2019，30，1： | 2 |
| 18 | Molecular cloning, tissue expression and protein structure prediction of the porcine 3-hydroxy-3-methylglutaryl-coenzyme A reductase ( HMGR ) gene显示文摘 | Xiao Chen Xiaofeng Wang Zongjin Li Lujun Kong Guifen Liu Jinluan Fu Aiguo Wang | 2011 | Gene2011，，2： | 2 |
| 19 | 最新版海冰模式CICE的优势:基于北极海冰时空特征的评估显示文摘【研究目的】海冰模式CICE(Los Alamos sea ice model)作为当前国际上的主流海冰模式之一,已被耦合进了大部分地球系统模式,对该模式模拟能力的评估工作是发展地球系统模式的重要参考依据。【创新点】通过观测数据与不同版本CICE模式对北极海冰数值模拟结果进行对比分析,研究了最新版本CICE6.0模拟能力及优势。【重要结论】CICE6.0模拟结果的年际误差最小,且季节变化与观测值最为吻合。相较而言,CICE4.0严重高估了冬季海冰总面积及低估了夏季海冰总面积,而CICE5.0在冬季的误差明显大于其他版本。此外,我们也关注了三个模式对多年冰和季节冰的模拟效果,从其均方根误差空间分布看出:模拟误差主要出现在中央海区及其周边海域。CICE4.0和CICE5.0在这些区域模拟的多年冰偏少、季节冰偏多,均低估了多年冰的变化趋势,且高估了季节冰的变化趋势;CICE6.0很好地解决了这些问题,其模拟的多年冰和季节冰的趋势最接近观测值,特别在北冰洋中部。总的来说,CICE6.0模拟的北极海冰在各方面都优于其他版本。 | WANG Huazhao ZHANG Lujun CHU Min HU Siyu | 2020 | Atmospheric and Oceanic Science Letters2020，13，2： | 2 |
| 20 | EGFR tyrosine kinase inhibitor HS-10182 increases radiation sensitivity in non-small cell lung cancers with EGFR T790M mutation显示文摘Objective:To investigate the potential of HS-10182,a second-generation epidermal growth factor receptor tyrosine kinase inhibitor(EGFR-TKI),as a radiosensitizer in non-small cell lung cancer(NSCLC).Methods:Two cell lines of NSCLCs,A549 that possesses wild-type(WT)EGFRs and H1975 that possesses EGFR L858R/T790M double mutations,were treated with HS-10182 at various concentrations,and cell viabilities were determined using the MTS assay.The cells were tested by clonogenic survival assays to identify the radiosensitivity of both groups.Western blot was performed to analyze the expression of phosphorylated EGFR,AKT,DNA-dependent protein kinase,and catalytic subunit(DNA-PKcs)proteins.Immunofluorescence analyses were performed to examine the formation and changes in nuclearγ-H2AX foci.Cell apoptosis was examined by flow cytometry and Western blots for cleaved caspase-3,-8,-9,and cleaved poly ADP-ribose polymerase(PARP).Furthermore,we established xenograft models in mice and the effects of different treatments on tumor growth were then assessed.Results:Clonogenic survival assays revealed that HS-10182 significantly enhanced the radiosensitivity of H1975 cells but not A549cells[dose enhancement ratios(DERs)=2.36(P<0.05)vs.1.43(P>0.05)].Western blot results showed that HS-10182 increased the levels of cleaved caspase-3,-8,-9,and cleaved PARP in H1975 cells but not in A549 cells.In addition,flow cytometry analysis showed that HS-10182 enhanced irradiation-induced apoptosis in H1975.Immunofluorescence results found that HS-10182increased the average number ofγ-H2AX foci after irradiation in H1975 cells,but not in A549 cells.Combined radiation and HS-10182 treatment increased the expression of DNA-PKcs but this increase was more significant in H1975 cells than in A549 cells.Moreover,HS-10182 suppressed the increased expression of Rad50 in H1975 cells in response to irradiation.In vivo experiments found that the combined therapy significantly inhibited tumor growth.Conclusions:HS-10182 enhances the radiosensitivity of H1975 cells which is possibly because that HS-10182 could enhance irradiation-induced apoptosis,increase irradiation-induced DNA damage,and cause a delay in DNA damage repair.Our findings suggest that radiotherapy combined HS-10182 is a novel treatment for lung cancer cells which have acquired the T790M mutation.HS-10182 could be brought to the clinic as a radiosensitizer in NSCLCs with the EGFR T790M mutation. | Yang Chen Youyou Wang Lujun Zhao Ping Wang Jifeng Sun Rudi Bao Chenghai Li Ningbo Liu | 2018 | Cancer Biology & Medicine2018，15，1： | 2 |
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