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| 1 | Sirolimus inhibits growth of human hepatoma cells alone or combined with tacrolimus, while tacrolimus promotes cell growth显示文摘AIM: Standard immunosuppression after organ transplantation stimulates tumor growth. Sirolimus has a strong antiproliferative and a tumor inhibiting effect. The purpose is to assess the effect on tumor growth of the immunosuppressive compounds sirolimus and tacrolimus alone and in combination on cells of human hepatocellular carcinoma.METHODS: We used the human cell lines SK-Hep 1 and Hep 3B derived from hepatocellular carcinoma. Proliferation analyses after treatment with sirolimus, tacrolimus, or the combination of both were performed. FACS analyses were done to reveal cell cycle changes and apoptotic cell death. The expression of apoptosis-related proteins was estimated by Western blots.RESULTS: Sirolimus alone or combined with tacrolimus inhibited the growth of both cell lines after 5 d by up to 35% in SK-Hep 1 cells, and by up to 68% in Hep 3B cells at 25 ng/mL. Tacrolimus alone stimulated the growth by 12% after 5 ng/mL and by 25% after 25 ng/mL in Hep 3B cells. We found an increase of apoptotic Hep 3B cells from 6 to 16%, and a G1-arrest in SK-Hep 1 cells with an increase of cells from 61 to 82%, when sirolimus and tacrolimus were combined. Bcl-2 was down-regulated in Hep 3B, but not in SK-Hep 1 cells after combined treatment.CONCLUSION: Sirolimus appears to inhibit the growth of hepatocellular carcinoma cells alone and in combination with tacrolimus. Sirolimus seems to inhibit the growth stimulation of tacrolimus. | Guido Schumacher Marijke Oidtmann Anne Rueggeberg Dietmar Jacob Sven Jonas Jan M. Langrehr Ruth Neuhaus Marcus Bahra Peter Neuhaus | 2005 | World Journal of Gastroenterology2005，11，10： | 14 |
| 2 | Extended surgical resection for xanthogranulomatous cholecystitis mimicking advanced gallbladder carcinoma: A case report and review of literature显示文摘Xanthogranulomatous 胆汁(XGC ) 是胆囊的破坏煽动性的疾病,很少包含邻近的机关并且模仿国王先进胆囊癌。诊断在病理学的检查以后仅仅通常是可能的。46 42 岁的女人被指我们包含肝核,正确的肝脑叶,恰好结肠的弯曲,和十二指肠的怀疑的胆囊癌症的中心。刷细胞学获得了由内视镜后退胆管造影术(ERC ) 出现了高级发育异常。病人经历了团的在团体切除术,由正确叶切除术,正确的半结肠切除术,和部分十二指肠的切除术组成。出人意料地揭示的病理学的检查一 XGC.Only,为有邻近的机关的直接参与的 XGC 的扩大外科的切除术的六个案例到目前为止被报导了。在这些情况中,与癌给 XGC 的可能的共存,恶意不能被排除,甚至在细胞学和 intraoperative 冰冻切片调查以后。在结论,由于源于在另外的方面上包围机关的高度好攻击的煽动性的侵略的一个方面和可能的复杂并发症上的胆囊癌的差的预后,它似乎这些案例应该被当作恶性瘤直到不那样证明。临床医生们应该包括 XGC 在之中可能微分在肝核群众诊断。 | Antonino Spinelli Guido Schumacher Andreas Pascher Enrique Lopez-Hanninen Hussain Al-Abadi Christoph Benckert Igor M Sauer Johann Pratschke Ulf P Neumann Sven Jonas Jan M Langrehr Peter Neuhaus | 2006 | World Journal of Gastroenterology2006，12，14： | 13 |
| 3 | Evolution of indications and results of liver transplantation in Europe. A report from the European Liver Transplant Registry (ELTR)显示文摘 | René Adam Vincent Karam Valérie Delvart John O’Grady Darius Mirza Jurgen Klempnauer Denis Castaing Peter Neuhaus Neville Jamieson Mauro Salizzoni Stephen Pollard Jan Lerut Andreas Paul Juan Carlos Garcia-Valdecasas Fernando San Juan Rodríguez Andrew Burro | 2012 | Journal of Hepatology2012，，3： | 6 |
| 4 | Different cava reconstruction techniques in liver transplantation:piggyback versus cava resection显示文摘BACKGROUND: Originally, cava reconstruction(CR) in liver transplantation meant complete resection and reinsertion of the donor cava. Alternatively, preservation of the recipients inferior vena cava(IVC) with side-to-side anastomosis(known as 'piggyback') can be performed. Here, partial clamping maintains blood flow of the IVC, which may improve cardiovascular stability, reduce blood loss and stabilize kidney function. The aim of this study was to compare both techniques with particular focus on kidney function.METHODS: A series of 414 patients who had had adult liver transplantations(2006-2009) were included. Among them,176(42.5%) patients had piggyback and 238 had classical CR operation, 112(27.1%) of the patients underwent CR accompanied with veno-venous bypass(CR-B) and 126(30.4%)without a bypass. The choice of either technique was based on the surgeons' individual preference. Kidney function [serum creatinine, calculated glomerular filtration rate(GFR), RIFLE stages] was assessed over 14 days.RESULTS: Lab-MELD scores were significantly higher in CR-B(22.5±11.0) than in CR(17.3±9.0) and piggyback(18.8±10.0)(P=0.008). Unexpectedly, the incidences of arterial stenoses(P=0.045) and biliary leaks(P=0.042) were significantly increased in piggyback. Preoperative serum creatinine levels were the highest in CR-B [1.45±1.17 vs 1.25±0.85(piggyback)and 1.13±0.60 mg/dL(CR); P=0.033]. Although a worsening of postoperative kidney function was observed among all groups,this was most pronounced in CR-B [creatinine day 14: 1.67±1.40 vs 1.35±0.96(piggyback) and 1.45±1.03 mg/dL(CR); P=0.102].Accordingly, the proportion of patients displaying RIFLE stages≥2 was the highest in CR/CR-B(26%/19%) when compared to piggyback(18%).CONCLUSIONS: Piggyback revealed a shorter warm ischemic time, a reduced blood loss, and a decreased risk of acute kidney failure. Thus, piggyback is a useful technique, which should be applied in standard procedures. When piggyback is unfeasible,cava replacement, which displayed a lower incidence of vascular and biliary complications in our study, remains as a safe alternative. | Volker Schmitz Wenzel Schoening Ines Jelkmann Brigitta Globke Andreas Pascher Marcus Bahra Peter Neuhaus Gero Puhl | 2014 | Hepatobiliary & Pancreatic Diseases International2014，13，3： | 5 |
| 5 | Predicting survival after liver transplantation in patients with hepatocellular carcinoma beyond the Milan criteria: a retrospective, exploratory analysis显示文摘 | Vincenzo Mazzaferro Josep M Llovet Rosalba Miceli Sherrie Bhoori Marcello Schiavo Luigi Mariani Tiziana Camerini Sasan Roayaie Myron E Schwartz Gian Luca Grazi René Adam Peter Neuhaus Mauro Salizzoni Jordi Bruix Alejandro Forner Luciano De Carlis Umberto | 2009 | Lancet Oncology2009，，1： | 4 |
| 6 | Vascular invasion and histopathologic grading determine outcome after liver transplantation for hepatocellular carcinoma in cirrhosis显示文摘 | Sven Jonas Wolf O. Bechstein Thomas Steinmüller Martin Herrmann Cornelia Radke Thomas Berg Utz Settmacher Peter Neuhaus | 2001 | Hepatology2001，，5： | 4 |
| 7 | Normalised intrinsic mortality risk in liver transplantation: European Liver Transplant Registry study显示文摘 | René Adam Valérie Cailliez Pietro Majno Vincent Karam Paul McMaster Roy Y Calne John O’Grady Rudolf Pichlmayr Peter Neuhaus Jean-Bernard Otte Krister Hoeckerstedt Henri Bismuth | 2000 | The Lancet . 2000 (9230)2000，，9230： | 3 |
| 8 | Megacolon in adulthood after surgical treatment of Hirschsprung's disease in early childhood显示文摘Hirschsprung's disease (HD) is a disorder associated with congenital malformation of the enteric nervous system with segmental aganglionosis. Prevailing therapy includes a resection of the affected part of the bowel. However, patients often do not obtain complete functional improvement after surgical treatment. We present the case of a 25-year-old woman who had surgical treatment of HD in early childhood. After that procedure she had clinical features of constipation for years in the end,passing of stool once a week, requiring laxatives and enemas. We diagnosed an incomplete resection of the aganglionic bowel via rectal biopsy and resected the remaining aganglionic segment. Two months after surgery the patient's bowel function improved to a frequency of 1-4 stools per day. We conclude that regular follow-up is required to identify HD patients with persistent alterations of bowel function after surgery. In patients presenting with constipation, recognition of a remaining aganglionic segment or other alterations of the enteric nervous system should be aimed at in an early stage. | Christoph R.Werner Gisela Stoltenburg-Didinger Henning Weidemann Christoph Benckert Marco Schmidtmann Ivo R.van der Voort Viola Andresen Burghard F.Klapp Peter Neuhaus Bertram Wiedenmann Hubert Mnnikes | 2005 | World Journal of Gastroenterology2005，11，36： | 3 |
| 9 | Surgical management of proximal bile duct cancer: extended right lobe resection increases resectability and radicality显示文摘 | Peter Neuhaus Sven Jonas Utz Settmacher Armin Thelen Christoph Benckert Enrique Lopez-H?nninen Rainer E. Hintze | 2003 | Langenbeck’s Archives of Surgery2003，，3： | 2 |
| 10 | Evolution of liver transplantation in Europe: Report of the European Liver Transplant Registry显示文摘 | René Adam Paul McMaster John G. O’Grady Denis Castaing Jurgen L. Klempnauer Neville Jamieson Peter Neuhaus Jan Lerut Mauro Salizzoni Stephen Pollard Ferdinand Muhlbacher Xavier Rogiers Juan Carlos Garcia Valdecasas Joaquin Berenguer Daniel Jaeck Enrique M | 2003 | Liver Transplantation2003，，12： | 2 |
| 11 | Oncological Superiority of Hilar En Bloc Resection for the Treatment of Hilar Cholangiocarcinoma显示文摘 | Peter Neuhaus Armin Thelen Sven Jonas Gero Puhl Timm Denecke Wilfried Veltzke-Schlieker Daniel Seehofer | 2012 | Annals of Surgical Oncology2012，，5： | 2 |
| 12 | Liver transplantation for HBV-related cirrhosis in Europe: An ELTR study on evolution and outcomes显示文摘 | Patrizia Burra Giacomo Germani Renè Adam Vincent Karam Alfredo Marzano Pietro Lampertico Mauro Salizzoni Franco Filipponi Jurgen L. Klempnauer Denis Castaing Murat Kilic Luciano De Carlis Peter Neuhaus Sezai Yilmaz Andreas Paul Antonio D. Pinna Andrew K. | 2012 | Journal of Hepatology2012，，： | 2 |
| 13 | Evolution of liver transplantation in Europe: Report of the European Liver Transplant Registry显示文摘 | René Adam Paul McMaster John G. O’Grady Denis Castaing Jurgen L. Klempnauer Neville Jamieson Peter Neuhaus Jan Lerut Mauro Salizzoni Stephen Pollard Ferdinand Muhlbacher Xavier Rogiers Juan Carlos Garcia Valdecasas Joaquin Berenguer Daniel Jaeck Enrique M | 2003 | Liver Transplantation2003，，12： | 2 |
| 14 | Increasing applicability of liver transplantation for patients with hepatitis B–related liver disease显示文摘 | Thomas Steinmüller Daniel Seehofer Nada Rayes Andrea R. Müller Utz Settmacher Sven Jonas Ruth Neuhaus Thomas Berg Uwe Hopf Peter Neuhaus | 2002 | Hepatology2002，，6： | 2 |
| 15 | Gender-dependent association of CTLA4 polymorphisms with resolution of hepatitis C virus infection显示文摘 | Eckart Schott Heiko Witt Holger Hinrichsen Konrad Neumann Viola Weich Alexandra Bergk Juliane Halangk Tobias Müller Sebastian Tinjala Gero Puhl Peter Neuhaus Bertram Wiedenmann Thomas Berg | 2006 | Journal of Hepatology2006，，3： | 2 |
| 16 | Multidisciplinary strategies to improve treatment outcomes in hepatocellular carcinoma: a European perspective显示文摘 | Massimo Colombo Jean-Luc Raoul Riccardo Lencioni Peter R. Galle Jessica Zucman-Rossi Rafael Ba?ares Daniel Seehofer Peter Neuhaus Philip Johnson | 2013 | European Journal of Gastroenterology & Hepatology2013，，6： | 2 |
| 17 | Staging of Klatskin tumours (hilar cholangiocarcinomas): comparison of MR cholangiography, MR imaging, and endoscopic retrograde cholangiography显示文摘 | Thomas J. Vogl Wolfram O. Schwarz Matthias Heller Christopher Herzog Stephan Zangos Rainer E. Hintze Peter Neuhaus Renate M. Hammerstingl | 2006 | European Radiology2006，，10： | 2 |
| 18 | Simultaneous versus staged liver resection of synchronous liver metastases from colorectal cancer显示文摘 | Armin Thelen Sven Jonas Christoph Benckert Antonino Spinelli Enrico Lopez-H?nninen Birgit Rudolph Ulf Neumann Peter Neuhaus | 2007 | International Journal of Colorectal Disease2007，，10： | 2 |
| 19 | Increasing applicability of liver transplantation for patients with hepatitis B–related liver disease显示文摘 | Thomas Steinmüller Daniel Seehofer Nada Rayes Andrea R. Müller Utz Settmacher Sven Jonas Ruth Neuhaus Thomas Berg Uwe Hopf Peter Neuhaus | 2002 | Hepatology2002，，6： | 2 |
| 20 | Warm HTK donor pretreatment reduces liver injury during static cold storage in experimental rat liver transplantation显示文摘BACKGROUND: Organ shortage has led to an increased number of transplantations from extended criteria donors. These organs are more vulnerable to ischemia-reperfusion injury. Thus,improvement of organ preservation is needed. HTK is a widely used preservation solution for static cold storage in liver transplantation. The present study was to investigate the beneficial effect of warm HTK donor pretreatment on liver preservation.METHODS: Male inbred Wistar rats(weighing 230-260 g) served as donors and recipients(n=6/group). Donors of treatment groups received i.v. 0.01 m L/g body weight(BW) warm(21 ℃) HTK systemically 15 minutes prior to cold perfusion. Control groups received 0.01 m L/g BW warm(21 ℃) Na Cl 0.9%. Following pretreatment,donors were flushed with 4 ℃ cold HTK,livers were explanted and stored in 4 ℃ HTK for six hours. Thereafter orthotopic liver transplantation was performed. Recipients were harvested four hours,two and five days after reperfusion and blood and liver tissue samples were obtained. Blood samples were analyzed for AST,ALT,lactate dehydrogenase and bilirubin. Liver histological analysis as well as tissue analysis for pro-MMP2,MMP2 and pro-MMP9 using zymography was conducted.RESULTS: Treatment groups showed significantly lower ALT and lactate dehydrogenase levels as well as significantly lower activities of pro-MMP2,MMP2 and pro-MMP9. Histological analysis revealed only minor damage in all groups.CONCLUSIONS: The new concept of warm HTK pretreatment significantly reduced ischemia-reperfusion injury. The reduced ischemia-reperfusion injury was due to MMP inhibition. Warm HTK donor pretreatment is easy to handle and could further improve HTK's potency in liver preservation. | Wenzel Schoening Veeravorn Ariyakhagorn Thomas Schubert Peter Olschewski Andreas Andreou Peter Neuhaus Johann Pratschke Gero Puhl | 2015 | Hepatobiliary & Pancreatic Diseases International2015，14，6： | 2 |
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