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| 1 | De novo characterization of the root transcriptome of a traditional Chinese medicinal plant Polygonum cuspidatum显示文摘Various active components have been extracted from the root of Polygonum cuspidatum.However,the genetic basis for their activity is virtually unknown.In this study,25600002 short reads (2.3 Gb) of P.cuspidatum root transcriptome were obtained via Illumina HiSeq 2000 sequencing.A total of 86418 unigenes were assembled de novo and annotated.Twelve,18,60 and 54 unigenes were respectively mapped to the mevalonic acid (MVA),methyl-D-erythritol 4-phosphate (MEP),shikimate and resveratrol biosynthesis pathways,suggesting that they are involved in the biosynthesis of pharmaceutically important anthraquinone and resveratrol.Eighteen potential UDP-glycosyltransferase unigenes were identified as the candidates most likely to be involved in the biosynthesis of glycosides of secondary metabolites.Identification of relevant genes could be important in eventually increasing the yields of the medicinally useful constituents of the P.cuspidatum root.From the previously published transcriptome data of 19 non-model plant taxa,1127 shared orthologs were identified and characterized.This information will be very useful for future functional,phylogenetic and evolutionary studies of these plants. | HAO DaCheng MA Pei MU Jun CHEN ShiLin XIAO PeiGen PENG Yong HUO Li XU LiJia SUN Chao | 2012 | Science China(Life Sciences)2012，55，5： | 36 |
| 2 | Chinese Multidisciplinary Expert Consensus on the Diagnosis and Treatment of Hyperuricemia and Related Diseases显示文摘 | Zou, He-Jian Wu, Hu-Sheng Zhou, Jing-Guo Zeng, Xue-Jun Dai, Lie Wu, Hua-Xiang Zhu, Xiao-Xia Mei, Chang-Lin Hao, Chuan-Ming Chen, Nan Liu, Bi-Cheng Chen, Jiang-Hua Yang, Li Nie, Jing Yu, Chen Peng, Ai Yu, Sheng-Qiang Li, Lin Ge, Jun-Bo Huo, Yong Zhang, Shu-Yang Chen, Yun-Dai Dong, Yu-Gang Liang, Chun Dai, Yu-Xiang Gao, Xin Li, Chang-Gui Zhao, Jia-Jun Chen, Hai-Bing Cheng, Zhi-Feng Lin, Huan-Dong Guan, Yang-Tai Wang, Kai Luo, Ben-Yan Dai, Ruo-Lian Jiang, Quan Xue, Luan Liang, Chao-Chao Chen, Ming Fan, Song | 2017 | Chinese Medical Journal2017，，20： | 30 |
| 3 | Chinese Trauma Surgeon Association for management guidelines of vacuum sealing drainage application in abdominal surgeries-Update and systematic review显示文摘Vacuum sealing drainage (VSD) is frequently used in abdominal surgeries. However, relevant guidelines are rare. Chin ese Trauma Surge on Associati on orga nized a committee composed of 28 experts across China in July 2017, aiming to provide an evidence-based recommendation for the application of VSD in abdominal surgeries.Eleven questions regarding the use of VSD in abdominal surgeries were addressed:(1) which type of materials should be respectively chosen for the intraperitoneal cavity, retroperitoneal cavity and superficial incisions?(2) Can VSD be preventively used for a high-risk abdominal incision w让h primary suture?(3) Can VSD be used in severely contaminated/infected abdominal surgical sites?(4) Can VSD be used for temporary abdominal cavity closure under some special conditions such as severe abdominal trauma, infection, liver transplantation and intra-abdominal volume increment in abdominal compartment syndrome?(5) Can VSD be used in abdominal organ inflammation, injury, or postoperative drainage?(6) Can VSD be used in the treatment of intestinal fistula and pancreatic fistula?(7) Can VSD be used in the treatment of intra-abdominal and extra-peritoneal abscess?(8) Can VSD be used in the treatment of abdominal wall wounds, wound cavity, and defects?(9) Does VSD in crease the risk of bleeding?(10) Does VSD increase the risk of intestinal wail injury?(11) Does VSD increase the risk of peritoneal adhesion? Focusing on these questions, evidence-based recommendations were given accordingly. VSD was strongly recommended regarding the questions 2-4. Weak recommendations were made regarding questions 1 and 5-11. Proper use of VSD in abdominal surgeries can lower the risk of infection in abdominal incisions with primary suture, treat severely contaminated/infected surgical sites and facilitate temporary abdominal cavity closure. | Yang Li Pei-Yuan Li Shi-Jing Sun Yuan-Zhang Yao Zhan-Fei Li Tao Liu Fan Yang Lian-Yang Zhang Xiang-Jun Bai Jing-Shan Huo Wu-Bing He Jun Ouyang Lei Peng Ping Hu Yan-An Zhu Ping Jin Qi-Feng Shao Yan-Feng Wang Rui-Wu Dai Pei-Yang Hu Hai-Ming Chen Ge-Fei Wang Yong-Gao Wang Hong-Xu Jin Chang-Ju Zhu Qi-Yong Zhang Biao Shao Xi-Guang Sang Chang-Lin Yin | 2019 | Chinese Journal of Traumatology2019，22，1： | 26 |
| 4 | Alisol B 23-acetate protects against non-alcoholic steatohepatitis in mice via farnesoid X receptor activation显示文摘Alisol B 23 醋酸盐(AB23A ) 是从繁体中文药根茎 alismatis 孤立的自然 triterpenoid,它展出很多项药理学活动,包括反肝炎病毒,反癌症和抗菌剂效果。在这研究,我们检验了 AB23A 是否在位于保护的效果下面的老鼠,和机制免于非酒精的 steatohepatitis (NASH ) 。NASH 在喂的老鼠被导致蛋氨酸并且胆碱缺乏(MCD ) 节食 4 个星期。老鼠同时与 AB23A 被对待(15, 30,和 60 mg ? 潦吗? | Qiang MENG Xing-ping DUAN Chang-yuan WANG Zhi-hao LIU Peng-yuan SUN Xiao-kui HUO Hui-jun SUN Jin-yong PENG Ke-xin LIU | 2017 | Acta Pharmacologica Sinica2017，38，1： | 24 |
| 5 | PERSPECTIVES IN MECHANICS OF HETEROGENEOUS SOLIDS显示文摘The Microand Nano-mechanics Working Group of the Chinese Society of Theoretical and Applied Mechanics organized a forum to discuss the perspectives,trends,and directions in mechanics of heterogeneous materials in January 2010.The international journal,Acta Mechanica Solida Sinica,is devoted to all fields of solid mechanics and relevant disciplines in science,technology,and engineering,with a balanced coverage on analytical,experimental,numerical and applied investigations.On the occasion of the 30 th anniversary of Acta Mechanica Solida Sinica,its editor-in-chief,Professor Q.S.Zheng invited some of the forum participants to review the state-of-the-art of mechanics of heterogeneous solids,with a particular emphasis on the recent research development results of Chinese scientists.Their reviews are organized into five research areas as reported in different sections of this paper.§I firstly brings in focus on microand nano-mechanics,with regards to several selective topics,including multiscale coupled models and computational methods,nanocrystal superlattices,surface effects,micromechanical damage mechanics,and microstructural evolution of metals and shape memory alloys.§II shows discussions on multifield coupled mechanical phenomena,e.g.,multi-fields actuations of liquid crystal polymer networks,mechanical behavior of materials under radiations,and micromechanics of heterogeneous materials.In §III,we mainly address the multiscale mechanics of biological nanocomposites,biological adhesive surface mechanics,wetting and dewetting phenomena on microstructured solid surfaces.The phononic crystals and manipulation of elastic waves were elaborated in §IV.Finally,we conclude with a series of perspectives on solid mechanics.This review will set a primary goal of future science research and engineering application on solid mechanics with the effort of social and economic development. | C.Q. Chen J.Z. Cui H.L. Duan X.Q. Feng L.H. He G.K. Hu M.J. Huang Y.Z. Huo B.H. Ji B. Liu X.H. Peng H.J. Shi Q.P. Sun J.X. Wang Y.S. Wang H.P. Zhao Y.P. Zhao Q.S. Zheng W.N. Zou | 2011 | Acta Mechanica Solida Sinica2011，24，1： | 18 |
| 6 | Enhanced rectification, transport property and photocurrent generation of multilayer ReSe2/MoS2 p-n heterojunctions显示文摘 | Xiaoting Wang Le Huang Yuting Peng Nengjie Huo Kedi Wu Congxin Xia Zhongming Wei Sefaattin Tongay Jingbo Li | 2016 | Nano Research2016，9，2： | 16 |
| 7 | Effects of TMCP Schedule on Precipitation,Microstructure and Properties of Ti-microalloyed High Strength Steel显示文摘Using the similar compositions of the Ti-microalloyed high-strength steels produced by the thin-slab casting process of compact strip production(CSP),four thermo-mechanical control processes(TMCP)after the simulated thickslab casting,i.e.the two hot rolling routes and the two cooling processes,were designed,aiming at achieving the same mechanical properties as the thin strip products.The final microstructures after the four TMCP processes were examined by optical microscope(OM),scanning electron microscope(SEM)and transmission electron microscope(TEM).The tensile properties and Charpy impact energy were measured correspondingly.Strain-induced TiC precipitation was found in the two-stage rolling route with the finish rolling temperature at low levels,leading to grain refinement due to the pinning effect during austenite recrystallization.Precipitation hardening in ferrite was observed when a period of isothermal holding was applied after hot rolling.It could be concluded that both finish rolling temperature and the subsequent isothermal holding temperature were crucial for the achieved strength level due to the combined effect of grain refinement and precipitation hardening.At the same time,it was found that the isothermal holding led to poor impact toughness because of remarkable precipitation hardening.Therefore,it was suggested that the precipitation kinetics of titanium carbides in both austenite and ferrite should be investigated in future. | Xiang-dong HUO Lie-jun LI Zheng-wu PENG Song-jun CHEN | 2016 | Journal of Iron and Steel Research(International)2016，23，6： | 11 |
| 8 | Efficacy and safety of transcatheter chemoembolization of the internal thoracic artery in patients with hepatocellular carcinoma显示文摘并行的动脉的背景 Embolization 为 transcatheter 是重要的肝的动脉的 chemoembolization (不作声) 在有 hepatocellular 癌(HCC ) 的病人。我们评估了功效和安全不作声,并且在内部胸的动脉( ITA )是 involved.Methods A 的在病人之中的复杂并发症的预防和管理 3614 总计 HCC 的盒子被对待与 12 645 不作声并且 211 个这些盒子被给 ITA angiography ,包括 ITA 被包含的 156 个盒子。我们执行了 562 在 156 个盒子中不作声。我们分析了成像考试, embolization 的类型,和发生,预防,和 ITA 是的 complications.Results 的治疗成功地在 156 种情况中的 embolized。ITA 的 Angiography 证明主要箱子与分叉的容器的一个增加的数字被变厚,贡献 intrahepatic 和 extrahepatic 肿瘤血供应。不同 embolization 方法根据血供应被选择,到有效地栓塞肿瘤并且减轻或避免严肃的复杂并发症。不作声, ITA embolization 延长了没有 ITA embolization.The ITA,在到 4.23 个月(1-30 月) 的从 2.54 个月(1-17 月) 的二治疗之间的吝啬的间隔时间在下列例子与那相比供应了 HCC:HCC 位于腹的肝的区域并且邻接了隔膜(P=0.0064 ) 并且重复不作声(P=0.0003 ) 。幸存率比没有 ITA embolization ( P 0.00001 ),不作声与 ITA embolization 不作声因为 HCC 好在有巨大的 HCC 或榴状的 HCC 的情况中的 .Conclusions , ITA 可以涉及供应血到 tumor.This 当肿瘤在腹的肝的区域被放并且邻接隔膜( S2 , S4 ,和 S8 )时,发生,并且特别如果盒子有以前的历史不作声。在这个情况系列, embolization 是有效的,扩大了 interventional 治疗的吝啬的间隔时间,并且延长了幸存时间。 | FAN Qing-sheng HUO Xiao-kun WANG Mao-qiang LIU Feng-yong DUAN Feng WANG Zhi-jun SONG Peng | 2011 | Chinese Medical Journal2011，，9： | 10 |
| 9 | Modified fertilization management of summer maize(Zea mays L.) in northern China improves grain yield and efficiency of nitrogen use显示文摘Improving the yield of maize grain per unit area is needed to meet the growing demand for it in China, where the availability of fertile land is very limited.Modified fertilization management and planting density are efficient methods for increasing crop yield.Field experiments were designed to investigate the influence of modified fertilization management and planting density on grain yield and nitrogen use efficiency of the popular maize variety Zhengdan 958, in four treatments including local farmer's practice(FP), high-yielding and high efficiency cultivation(HH), super high-yielding cultivation(SH), and the control(CK).Trials were conducted in three locations of the Huang-Huai-Hai Plain in northern China.Compared with FP, SH was clearly able to promote N absorption and dry matter accumulation in post-anthesis, and achieve high yield and N use efficiency by increasing planting density and postponing the supplementary application of fertilizers.However, with an increase in planting density, the demand of N increased along with grain yield.Due to the input of too much N fertilizer, the efficiency of N use in SH was low.Applying less total N, ameliorating cultivation and cropping management practices should be considered as priority strategies to augment production potential and finally achieve synchronization between high yield and high N efficiency in fertile soils.However, in situations where soil fertility is low, achieving high yield and high N use efficiency in maize will likely depend on increased planting density and appropriate application of supplementary fertilizers postpone to the grain-filling stage. | CHENG Yi ZHAO Jie LIU Zhen-xiang HUO Zhi-jin LIU Peng DONG Shu-ting ZHANG Ji-wang ZHAO Bin | 2015 | Journal of Integrative Agriculture2015，14，8： | 9 |
| 10 | Endovascular therapy for Acute ischemic Stroke Trial (EAST): study protocol for a prospective, multicentre control trial in China显示文摘Background and purpose:5 recent trials have shown the benefit of endovascular treatment for acute ischaemic stroke(AIS)due to large vessel occlusion of the anterior circulation.This study aims to evaluate the safety and efficacy of Solitaire thrombectomy in patients with moderate-to-severe stroke in the Chinese population,which has a high prevalence of intracranial atherosclerosis.Methods and analysis:This multicentre prospective control study will involve 17 stroke centres in China,and plans to recruit 150 patients in the intervention group,and 150 patients in the medical group,in which patients meet enrolment criteria but refuse intervention.Patients with AIS due to large vessel occlusion indicated for treatment with Solitaire stent retriever within 12 hours of symptom onset,and who meet the inclusion and exclusion criteria,will be enrolled in this study.The primary efficacy endpoint is functional independence as defined by a modified Rankin Scale(mRS)score≤2 at 90 days or by functional improvement as defined by mRS,using shift analysis.The procedural efficacy endpoint is arterial recanalisation of the occluded target vessel measured by a modified Thrombolysis in Cerebral Infarction(mTICI)score equal or superior to 2b right following the use of the study device.The primary safety endpoint is symptomatic intracranial haemorrhage(sICH)within 24±3 hours postprocedure.Ethics and dissemination:The protocol was approved by the Ethics Committee at the coordinating centre and by the local Institutional Review Board of each participating centre.Trial registration number:NCT02350283. | Zhongrong Miao Xiaochuan Huo Feng Gao Xiaoling Liao Chunjuan Wang Ya Peng Yibin Cao Shengli Chen Meng Zhang Changchun Jiang Xiaoxiang Peng Cunfeng Song Liping Wei Qiyi Zhu Zaiyu Guo Li Liu Hang Lin Hua Yang Wei Wu Hui Liang Anding Xu Kangning Chen Xingquan Zhao Yuesong Pan Hao Li Liping Liu Yilong Wang Yongjun Wang EAST investigators | 2016 | Stroke & Vascular Neurology2016，1，2： | 8 |
| 11 | Nine-month angiographic and two-year clinical follow-up of polymer-free sirolimus-eluting stent versus durable-polymer sirolimus-eluting stent for coronary artery disease: the Nano randomized trial显示文摘 | Zhang Yaojun Chen Fang Takashi Muramatsu Xu Bo Li Zhanquan Ge Junbo He Qing Yang Zhijian Li Shumei Wang Lefeng Wang Haichang He Ben Li Kang Qi Guoxian Li Tianchang Zeng Hesong Peng Jianjun Jiang Tieming Zeng Qiutang Zhu Jianhua Fu Guosheng Christos V. Bourantas Patrick W. Serruys Huo Yong | 2014 | Chinese Medical Journal2014，，11： | 8 |
| 12 | CHARACTERISTICS OF STRESS-INDUCED TRANSFORMATION AND MICROSTRUCTURE EVOLUTION IN Cu-BASED SMA显示文摘形状记忆合金(SMA ) 的机械行为是仔细与它的微观结构的形成和进化有关。通过理论分析和试验性的观察,导致压力的 martensitic 转变在单轴的紧张状况下面单个水晶处理基于 Cu 的 SMA,这被发现由三个时期组成了:成核,混合成核和生长,并且由于生长合并。在成核期间,压力很快落下,当阶段部分慢慢地增加了时,接口的数字很快增加了。在第二个时期,压力和接口数字稍微变化了,但是阶段部分戏剧性地增加了。最后,当接口的数字快速减少了时,压力和阶段部分慢慢地变化了。而且,转变能具有,这被发现多级式:在几紧张并且相应地的锋利的压力落下,成核和生长过程在样品的几部分伪独立地发生了。 | Cheng Peng Xingyao Wang Yongzhong Huo | 2008 | Acta Mechanica Solida Sinica2008，21，1： | 7 |
| 13 | Immunity-and-matrix-regulatory cells derived from human embryonic stem cells safely and effectively treat mouse lung injury and fibrosis显示文摘Lung injury and fibrosis represent the most significant outcomes of severe and acute lung disorders,including COVID-19.However,there are still no effective drugs to treat lung injury and fibrosis.In this study,we report the generation of clinical-grade human embryonic stem cells(hESCs)-derived immunity-and matrix-regulatory cells(IMRCs)produced under good manufacturing practice requirements,that can treat lung injury and fibrosis in vivo.We generate IMRCs by sequentially differentiating hESCs with serum-free reagents.IMRCs possess a unique gene expression profile distinct from that of umbilical cord mesenchymal stem cells(UCMSCs),such as higher expression levels of proliferative,immunomodulatory and anti-fibrotic genes.Moreover,intravenous delivery of IMRCs inhibits both pulmonary inflammation and fibrosis in mouse models of lung injury,and significantly improves the survival rate of the recipient mice in a dose-dependent manner,likely through paracrine regulatory mechanisms.IMRCs are superior to both primary UCMSCs and the FDA-approved drug pirfenidone,with an excellent efficacy and safety profile in mice and monkeys.In light of public health crises involving pneumonia,acute lung injury and acute respiratory distress syndrome,our findings suggest that IMRCs are ready for clinical trials on lung disorders. | Jun Wu Dingyun Song Zhongwen Li Baojie Guo Yani Xiao Wenjing Liu Lingmin Liang Chunjing Feng Tingting Gao Yanxia Chen Ying Li Zai Wang Jianyan Wen Shengnan Yang Peipei Liu Lei Wang Yukai Wang Liang Peng Glyn Nigel Stacey Zheng Hu Guihai Feng Wei Li Yan Huo Ronghua Jin Ng Shyh-Chang Qi Zhou Liu Wang Baoyang Hu Huaping Dai Jie Hao | 2020 | Cell Research2020，30，9： | 7 |
| 14 | Investigation of long-term implantation of BuMA stent in a porcine coronary model显示文摘 | CHEN Ming WANG Xin-gang ZHENG Bo PENG Hong-yu Zhang Xiao-yan ZHANG Bin HUO Yong | 2012 | Chinese Medical Journal2012，，22： | 6 |
| 15 | Spider web-like carbonized bacterial cellulose/MoSe_(2) nanocomposite with enhanced microwave attenuation performance and tunable absorption bands显示文摘It is essential to manufacture microwave absorbers with strong absorption as well as tunable absorption bands at a low filler content.However,it remains challenging for pure biomass material to reach this goal without loading other components.MoSe_(2),as a transition metal chalcogenide with semiconductor properties,has emerged as a potential microwave absorber filler.Herein,bacterial cellulose(BC)-derived carbon nanofibers/MoSe_(2) nanocomposite was fabricated and phosphoric acid was used to dope phosphorus in BC,in which MoSe_(2) microspheres were dropped on the BC network like a dew-covered spider web.This unique network structure enhances conductive loss and multiple reflections of the incident wave.The collocation of BC and MoSe_(2) is helpful to impedance match and introduces interfacial/dipolar polarization loss;moreover,the P-doping of BC helps to tune the absorption bands.Overall,the optimal reflection loss of undoped one reaches−53.33 dB with only 20 wt.%filler content,whose main absorption peaks focus on X-band.Interestingly,after the P-doping of BC,the main absorption peaks move to Ku-band and the optimal reflection loss gets stronger(−66.84 dB)with the same filler loading.Strong absorption and tunable absorption bands can be realized,and thus wide frequency range is covered.This work is expected to enlighten future exploration of biomass carbon materials on high-performance microwave absorption materials. | Zhengjian Xu Man He Yuming Zhou Shuangxi Nie Yongjuan Wang Yao Huo Yifan Kang Ruili Wang Ran Xu Hao Peng Xi Chen | 2021 | Nano Research2021，14，3： | 6 |
| 16 | Value of urine IL-8, NGAL and KIM-1 for the early diagnosis of acute kidney injury in patients with ureteroscopic lithotripsy related urosepsis显示文摘Purpose::To investigate the clinical value of urine interleukin-18(IL-8),neutrophil gelatinase-associated lipocalin(NGAL)and kidney injury molecule-1(KIM-1)for the early diagnosis of acute kidney injury(AKI)in patients with ureteroscopic lithotripsy(URL)related urosepsis.Methods::A retrospective study was carried out in 157 patients with urosepsis after URL.The patients were divided into AKI group and non-AKI group according to the Kidigo guideline and urine IL-8,NGAL and KIM-1 levels were detected by enzyme-linked immunosorbent assay at 0,4,12,24 and 48 h after the surgery.Receiver operating characteristic curve(ROC)was used to evaluate the diagnostic value of these three biomarkers for postoperative AKI.Results::The level of urine IL-8,NGAL and KIM-1 in AKI group was significantly higher than that in non-AKI group at 4,12,24 and 48 h(p<0.01).The ROC analysis showed the combined detection of urine IL-8,NGAL and KIM-1 at 12 h had a larger area under curve(AUC)than a single marker(0.997,95%CI:0.991-0.998),and the sensitivity and specificity were 98.2%and 96.7%,respectively.Pearson correlation analysis showed that the levels of urine NGAL at 4,12,24 and 48 h in AKI patients were positively correlated with the levels of urine KIM-1 and IL-18(p<0.01).Conclusion::AKI could be quickly recognized by the elevated level of urine IL-8,NGAL and KIM-1 in patients with URL-related urosepsis.Combined detection of the three urine biomarkers at 12 h after surgery had a better diagnostic performance,which may be an important reference for the early diagnosis of AKI. | Dan Tan Liang Zhao Wei Peng Fang-Hao Wu Guo-Bin Zhang Bo Yang Wen-Qian Huo | 2022 | Chinese Journal of Traumatology2022，25，1： | 6 |
| 17 | CD147 antibody specifically and effectively inhibits infection and cytokine storm of SARS-CoV-2 and its variants delta,alpha,beta,and gamma显示文摘SARS-CoV-2 mutations contribute to increased viral transmissibility and immune escape,compromising the effectiveness of existing vaccines and neutralizing antibodies.An in-depth investigation on COVID-19 pathogenesis is urgently needed to develop a strategy against SARS-CoV-2 variants.Here,we identified CD147 as a universal receptor for SARS-CoV-2 and its variants.Meanwhile,Meplazeumab,a humanized anti-CD147 antibody,could block cellular entry of SARS-CoV-2 and its variants-alpha,beta,gamma,and delta,with inhibition rates of 68.7,75.7,52.1,52.1,and 62.3%at 60μg/ml,respectively.Furthermore,humanized CD147 transgenic mice were susceptible to SARS-CoV-2 and its two variants,alpha and beta.When infected,these mice developed exudative alveolar pneumonia,featured by immune responses involving alveoli-infiltrated macrophages,neutrophils,and lymphocytes and activation of IL-17 signaling pathway.Mechanistically,we proposed that severe COVID-19-related cytokine storm is induced by a'spike protein-CD147-CyPA signaling axis':Infection of SARS-CoV-2 through CD147 initiated the JAK-STAT pathway,which further induced expression of cyclophilin A(CyPA);CyPA reciprocally bound to CD147 and triggered MAPK pathway.Consequently,the MAPK pathway regulated the expression of cytokines and chemokines,which promoted the development of cytokine storm.Importantly,Meplazumab could effectively inhibit viral entry and inflammation caused by SARS-CoV-2 and its variants.Therefore,our findings provided a new perspective for severe COVID-19-related pathogenesis.Furthermore,the validated universal receptor for SARS-CoV-2 and its variants can be targeted for COVID-19 treatment. | Jiejie Geng Liang Chen Yufeng Yuan Ke Wang Youchun Wang Chuan Qin Guizhen Wu Ruo Chen Zheng Zhang Ding Wei Peng Du Jun Zhang Peng Lin Kui Zhang Yongqiang Deng Ke Xu Jiangning Liu Xiuxuan Sun Ting Guo Xu Yang Jiao Wu Jianli Jiang Ling Li Kun Zhang Zhe Wang Jing Zhang Qingguo Yan Hua Zhu Zhaohui Zheng Jinlin Miao Xianghui Fu Fengfan Yang Xiaochun Chen Hao Tang Yang Zhang Ying Shi Yumeng Zhu Zhuo Pei Fei Huo Xue Liang Yatao Wang Qingyi Wang Wen Xie Yirong Li Mingyan Shi Huijie Bian Ping Zhu Zhi-Nan Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，10： | 6 |
| 18 | Effects of vitrification and cryostorage duration on single-cell RNA-Seq profiling of vitrified-thawed human metaphase Ⅱ oocytes显示文摘Oocyte cryopreservation is widely used for clinical and social reasons.Previous studies have demonstrated that conventional slow-freezing cryopreservation procedures,but not storage time,can alter the gene expression profiles of frozen oocytes.Whether vitrification procedures and the related frozen storage durations have any effects on the transcriptomes of human metaphase Ⅱ oocytes remain unknown.Four women(30–32 years old)who had undergone IVF treatment were recruited for this study.RNA-Seq profiles of 3 fresh oocytes and 13 surviving vitrified-thawed oocytes(3,3,4,and 3 oocytes were cryostored for 1,2,3,and 12 months)were analyzed at a single-cell resolution.A total of 1987 genes were differentially expressed in the 13 vitrifiedthawed oocytes.However,no differentially expressed genes were found between any two groups among the 1-,2-,3-,and 12-month storage groups.Further analysis revealed that the aberrant genes in the vitrified oocytes were closely related to oogenesis and development.Our findings indicated that the effects of vitrification on the transcriptomes of mature human oocytes are induced by the procedure itself,suggesting that long-term cryostorage of human oocytes is safe. | Ying Huo Peng Yuan Qingyuan Qin Zhiqiang Yan Liying Yan Ping Liu Rong Li Jie Yan Jie Qiao | 2021 | Frontiers of Medicine2021，15，1： | 6 |
| 19 | Mitochondrial uncoupler BAM15 inhibits artery constriction and potently activates AMPK in vascular smooth muscle cells显示文摘Our previous studies found that mitochondrial uncouplers CCCP and niclosamide inhibited artery constriction and the mechanism involved AMPK activation in vascular smooth muscle cells. BAM15 is a novel type of mitochondrial uncoupler. The aim of the present study is to identify the vasoactivity of BAM15 and characterize the BAM15-induced AMPK activation in vascular smooth muscle cells(A10 cells). BAM15 relaxed phenylephrine(PE)-induced constricted rat mesenteric arteries with intact and denuded endothelium. Pretreatment with BAM15 inhibited PEinduced constriction of rat mesenteric arteries with intact and denuded endothelium. BAM15, CCCP,and niclosamide had the comparable IC50 value of vasorelaxation in PE-induced constriction of rat mesenteric arteries. BAM15 was less cytotoxic in A10 cells compared with CCCP and niclosamide.BAM15 depolarized mitochondrial membrane potential, induced mitochondrial fission, increased mitochondrial ROS production, and increased mitochondrial oxygen consumption rate in A10 cells.BAM15 potently activated AMPK in A10 cells and the efficacy of BAM15 was stronger than that of CCCP, niclosamide, and AMPK positive activators metformin and AICAR. In conclusion, BAM15 activates AMPK in vascular smooth muscle cells with higher potency than that of CCCP, niclosamide and the known AMPK activators metformin and AICAR. The present work indicates that BAM15 is a potent AMPK activator. | Yu Tai Lanjun Li Xuan Peng Junxue Zhu Xihai Mao Nan Qin Minghui Ma Rong Huo Yunlong Bai Deli Dong | 2018 | Acta Pharmaceutica Sinica B2018，8，6： | 5 |
| 20 | Cilastatin protects against imipenem-induced nephrotoxicity via inhibition of renal organic anion transporters(OATs)显示文摘Imipenem is a carbapenem antibiotic. However, Imipenem could not be marketed owing to its instability and nephrotoxicity until cilastatin, an inhibitor of renal dehydropeptidase-I(DHP-I),was developed. In present study, the potential roles of renal organic anion transporters(OATs) in alleviating the nephrotoxicity of imipenem by cilastatin were investigated in vitro and in rabbits. Our results indicated that imipenem and cilastatin were substrates of h OAT1 and h OAT3. Cilastatin inhibited h OAT1/3-mediated transport of imipenem with IC50 values comparable to the clinical concentration, suggesting the potential to cause a clinical drug–drug interaction(DDI). Moreover,imipenem exhibited h OAT1/3-dependent cytotoxicity, which was alleviated by cilastatin and probenecid. Furthermore, cilastatin and probenecid ameliorated imipenem-induced rabbit acute kidney injury, and reduced the renal secretion of imipenem. Cilastatin and probenecid inhibited intracellular accumulation of imipenem and sequentially decreased the nephrocyte toxicity in rabbit primary proximal tubule cells. Renal OATs, besides DHP-I, was also the target of interaction between imipenem and cilastatin, and contributed to the nephrotoxicity of imipenem. This therefore gives in part the explanation about the mechanism by which cilastatin protected against imipenem-induced nephrotoxicity. Thus, OATs can potentially be used as a therapeutic target to avoid the renal adverse reaction of imipenem in clinic. | Xiaokui Huo Qiang Meng Changyuan Wang Yanna Zhu Zhihao Liu Xiaodong Ma Xiaochi Ma Jinyong Peng Huijun Sun Kexin Liu | 2019 | Acta Pharmaceutica Sinica B2019，9，5： | 5 |
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