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| 1 | Polysaccharides from Angelica sinensis alleviate neuronal cell injury caused by oxidative stress显示文摘Angelica sinensis has antioxidative and neuroprotective effects. In the present study, we aimed to determine the neuroprotective effect of polysaccharides isolated from Angelica sinensis. In a preliminary experiment, Angelica sinensis polysaccharides not only protected PC12 neuronal cells from H 2 O 2-induced cytotoxicity, but also reduced apoptosis and intracellular reactive oxygen species levels, and increased the mitochondrial membrane potential induced by H 2 O 2 treatment. In a rat model of local cerebral ischemia, we further demonstrated that Angelica sinensis polysaccharides enhanced the antioxidant activity in cerebral cortical neurons, increased the number of microvessels, and improved blood flow after ischemia. Our findings highlight the protective role of polysaccharides isolated from Angelica sinensis against nerve cell injury and impairment caused by oxidative stress. | Tao Lei Haifeng Li Zhen Fang Junbin Lin Shanshan Wang Lingyun Xiao Fan Yang Xin Liu Junjian Zhang Zebo Huang Weijing Liao | 2014 | Neural Regeneration Research2014，9，3： | 17 |
| 2 | Side lobes of wavelets impact identification of thin sand bodies显示文摘最近,当在西北中国在盆学习一些煤气的领域时,我们发现小浪的方面脑叶在薄沙水库的鉴定上有大影响。从 H 形成,在有煤气忍受的薄沙身体的顶的思考,有在二片弱山峰方面脑叶之间的主要小浪脑叶。总是,有另一座山峰的混合从一座薄沙水库的顶反映了的更低的。那使识别沙水库困难。以便解决这,许多前面的模型用典型的井木头被建立。2D 合成侧面用 Ricker 小浪被生产学习在小浪方面脑叶和薄沙位置和频率的效果之间并且在振幅和薄沙身体之间的关系。我们开发了下列结论:首先,在一个更浅的位置识别薄沙更容易。第二,把沙身体思考与方面脑叶区别开来的一个好方法由把侧面与不同频率窗口作比较。第三,用振幅属性描述沙程度有用、有效。 | Huang Junbin Gao Lijun Gao Yong | 2007 | Applied Geophysics2007，4，2： | 7 |
| 3 | Rational development of a human antibody cocktail that deploys multiple functions to confer Pan-SARS-CoVs protection显示文摘Structural principles underlying the composition and synergistic mechanisms of protective monoclonal antibody cocktails are poorly defined.Here,we exploited antibody cooperativity to develop a therapeutic antibody cocktail against SARS-CoV-2.On the basis of our previously identified humanized cross-neutralizing antibody H014,we systematically an a lyzed a fully human n aive antibody library and rationally identified a potent neutralizing antibody partner,P17,which confers effective protection in animal model.Cryo-EM studies dissected the nature of the P17 epitope,which is SARS-CoV-2 specific and distinctly different from that of HOI4.High-resolution structure of the SARS-CoV-2 spike in complex with HOM and P17,together with functional investigations revealed that in a two-antibody cocktail,synergistic neutralization was achieved by S1 shielding and conformational locking,thereby blocking receptor attachment and viral membrane fusion,conferring high potency as well as robustness against viral mutation escape.Furthermore,cluster analysis identified a hypothetical 3rd antibody partner for further reinforcing the cocktail as pan-SARS-CoVs therapeutics. | Hangping Yao Yao Sun Yong-Qiang Deng Nan Wang Yongcong Tan Na-Na Zhang Xiao-Feng Li Chao Kong Yan-Peng Xuc Qi Chen Tian-Shu Cao Hui Zhao Xintian Yan Lei Cao Zhe Lv Dandan Zhu Rui Feng Nanping Wu Wenhai Zhang Yuhao Hu Keda Chen Rong-Rong Zhang Qingyu Lv Shihui Sun Yunhua Zhou Run Yan Guan Yang Xinglu Chanjuan Liu Xiangyun Lu Linfang Cheng Hongying Qiu Xing-Yao Huang Tianhao Weng Danrong Shi Weidong Jiang Junbin Shao Lei Wang Jie Zhang Tao Jiang Guojun Lang Cheng-Feng Qinc Lanjuan Li Xiangxi Wang | 2021 | Cell Research2021，31，1： | 5 |
| 4 | Design and synthesis of a packaging polymer enhancing the sensitivity of fiber grating pressure sensor显示文摘A packaging polymer (PP-1) that can enhance the sensitivity of fiber Bragg grating (FBG) pressure sensor was designed and synthe-sized from hydroxyl terminated polypropylene oxide oligomers,toluene diisocyanate (TDI),1,4-butandiol as chain extender,catalyzer,foam agent and foam stabilizer. The testing results show that the Young's modulus,Poisson's ratio of the packaging polymer are 9.0 · 106 N m 2 and 0.49,respectively. The static pressure sensitivity of fiber optical Bragg gratings packaged by PP-1 was discussed. The theoretical pressure sensitivity of FBG packaged with the polymer cylinder is 1.73 · 10 3 MPa 1,which is 896 times of that of the bare FBG ( 1.93 · 10 6 MPa 1). And its measured pressure sensitivity is 1.10 · 10 3 MPa 1,which is 558 times of that of the bare FBG. | Qingzhen Wen Jinhua Zhu Shenguang Gong Junbin Huang Hongcan Gu Peizhong Zhao | 2008 | Progress in Natural Science:Materials International2008，18，2： | 3 |
| 5 | Influence of synthesis temperatures on the crystalline grain growth and morphology of lanthanum magnesium hexaaluminate显示文摘Lanthanum magnesium hexaaluminate(LaMgAl_(11)O_(19), LMA) was prepared at different temperatures by solid-state reaction. Phase compositions and crystal morphologies of specimens synthesized at different temperatures were investigated using X-ray diffraction(XRD), scanning electron microscopy(SEM). It was observed that the crystalline grain size of LMA was not only dependent on the preparation temperature but also on its powder morphology. In the temperature range of 1300 e1550℃, LMA showed platelet grain and the average crystalline grain size increases with the increase in temperature. At1600℃, if the powder was sintered for two times, the equiaxed grain could be found with the decrease in grain space, resulting in the reduction of the crystalline grain size. Styles of specimens(powder or disk) might have no obvious influence on morphologies and sizes of LMA crystalline grains which were synthesized with the well-dispersed raw material mixtures. The synthesis temperature played a key role in influencing the free space for the formation and growth of crystalline grains. | Junbin Sun Jinshuang Wang Wenzhi Huang Yu Hui Xin Zhou Lifen Li Jianing Jiang Longhui Deng Yunya Niu Shujuan Dong Xueqiang Cao | 2017 | Journal of Rare Earths2017，35，12： | 2 |
| 6 | Vision-Based Hand Gesture Recognition for Human-Computer Interaction——A Survey显示文摘Recently,vision-based gesture recognition(VGR)has become a hot research spot in human-computer interaction(HCI).Unlike other gesture recognition methods with data gloves or other wearable sensors,vision-based gesture recognition could lead to more natural and intuitive HCI interactions.This paper reviews the state-of-the-art vision-based gestures recognition methods,from different stages of gesture recognition process,i.e.,(1)image acquisition and pre-processing,(2)gesture segmentation,(3)gesture tracking,(4)feature extraction,and(5)gesture classification.This paper also analyzes the advantages and disadvantages of these various methods in detail.Finally,the challenges of vision-based gesture recognition in haptic rendering and future research directions are discussed. | GAO Yongqiang LU Xiong SUN Junbin TAO Xianglin HUANG Xiaomei YAN Yuxing LIU Jia | 2020 | Wuhan University Journal of Natural Sciences2020，25，2： | 2 |
| 7 | Histones released by NETosis enhance the infectivity of SARS-CoV-2 by bridging the spike protein subunit 2 and sialic acid on host cells显示文摘Neutrophil extracellular traps(NETs)can capture and kill viruses,such as influenza viruses,human immunodeficiency virus(HIV),and respiratory syncytial virus(RSV),thus contributing to host defense.Contrary to our expectation,we show here that the histones released by NETosis enhance the infectivity of SARS-CoV-2,as found by using live SARS-CoV-2 and two pseudovirus systems as well as a mouse model.The histone H3 or H4 selectively binds to subunit 2 of the spike(S)protein,as shown by a biochemical binding assay,surface plasmon resonance and binding energy calculation as well as the construction of a mutant S protein by replacing four acidic amino acids.Sialic acid on the host cell surface is the key molecule to which histones bridge subunit 2 of the S protein.Moreover,histones enhance cell-cell fusion.Finally,treatment with an inhibitor of NETosis,histone H3 or H4,or sialic acid notably affected the levels of sgRNA copies and the number of apoptotic cells in a mouse model.These findings suggest that SARS-CoV-2 could hijack histones from neutrophil NETosis to promote its host cell attachment and entry process and may be important in exploring pathogenesis and possible strategies to develop new effective therapies for COVID-19. | Weiqi Hong Jingyun Yang Jun Zou Zhenfei Bi Cai He Hong Lei Xuemei He Xue Li Aqu Alu Wenyan Ren Zeng Wang Xiaohua Jiang Kunhong Zhong Guowen Jia Yun Yang Wenhai Yu Qing Huang Mengli Yang Yanan Zhou Yuan Zhao Dexuan Kuang Junbin Wang Haixuan Wang Siyuan Chen Min Luo Ziqi Zhang Tiangi Lu Li Chen Haiying Que Zhiyao He Qiu Sun Wei Wang Guobo Shen Guangwen Lu Zhiwei Zhao Li Yang Jinliang Yang Zhenling Wang Jiong Li Xiangrong Song Lunzhi Dai Chong Chen Jia Geng Maling Gou Lu Chen Haohao Dong Yong Peng Canhua Huang Zhiyong Qian Wei Cheng Changfa Fan Yuquan Wei Zhaoming Su Aiping Tong Shuaiyao Lu Xiaozhong Peng Xiawei Wei | 2022 | Cellular & Molecular Immunology2022，19，5： | 2 |
| 8 | Comprehensive identification of lysine 2-hydroxyisobutyrylated proteins in Ustilaginoidea virens reveals the involvement of lysine 2-hydroxyisobutyrylation in fungal virulence显示文摘Lysine 2-hydroxyisobutyrylation(Khib) is a newly identified post-translational modification(PTM) that plays important roles in transcription and cell proliferation in eukaryotes. However, its function remains unknown in phytopathogenic fungi. Here,we performed a comprehensive assessment of Khibin the rice false smut fungus Ustilaginoidea virens, using Tandem Mass Tag(TMT)-based quantitative proteomics approach. A total of 3 426 Khibsites were identified in 977 proteins, sugg esting that Khibis a common and complex PTM in U. virens. Our data demonstrated that the2-hydroxyisobutyrylated proteins are involved in diverse biological processes. Network analysis of the modified proteins revealed a highly interconnected protein network that included many well-studied virulence factors. We confirmed that the Zn-binding reduced potassium dependency3-type histone deacetylase(UvRpd3) is a major enzyme that removes 2-hydroxyisobutyrylation and acetylation in U. virens. Notably, mutations of Khib sites in the mitogen-activated protein kinase(MAPK)UvSlt2 significantly reduced fungal virulence and decreased the enzymatic activity of UvSlt2. Molecular dynamics simulations demonstrated that 2-hydroxyisobutyrylation in UvSlt2 increased the hydrophobic solvent-accessible surface area and thereby affected binding between the UvSlt2 enzyme and its substrates. Our findings thus establish Khibas a major post-translational modification in U. virens and point to an important role for Khibin the virulence of this phytopathogenic fungus. | Xiaoyang Chen Xiabing Li Pingping Li Xiaolin Chen Hao Liu Junbin Huang Chaoxi Luo Tom Hsiang Lu Zheng | 2021 | Journal of Integrative Plant Biology2021，63，2： | 2 |
| 9 | Research of the planar optical fiber Bragg grating hydrophone probe显示文摘这篇论文论述平面纤维布拉格栅栏(FBG ) 水中听音器探查察觉到原则,并且理论上并且试验性地研究探查结构敏感,收到的敏感频率反应特征和加速反应性质。平面表用不锈钢做的,它的厚度是 0.15 公羊,它的直径是空圆形的壳的 15mm,和长度是 20 公羊。为结构的这种尺寸,探查结构敏感直到 23 fm/Pa,它是大约 7300 次赤裸的纤维的价值。回声频率是 6.5 kHz,和反应是的收到的敏感的振幅频率曲线相对在到 5.5 kHz 的 100 Hz 的频率范围以内的命令。一单位加速(1 m/s2 ) 产出的产量等价于(2.52 ~ 3.26 Pa ) 声学的压力代理输出。这探查结构是容易的由 multiplexing technique.The 研究表演形成 FBG 水中听音器数组这平面结构能不仅形成 FBG 水中听音器探查,而且能组成光 FBG 激光水中听音器探查。结构能认识到不同带宽,不同范围由调整表的几何尺寸的声学的压力测量。 | WANG Junjie JIANG Desheng XIE Guanmo: LIANG Yufei HUANG Junbin | 2008 | Chinese Journal of Acoustics2008，27，1： | 1 |
| 10 | Study of the planar optical fiber Bragg grating hydrophone probe with acceleration compensation显示文摘自从一个平面光 FBG 水中听音器的探查加速反应,是很明显的并且在正常上有不利影响在水下声学的压力察觉,采用一项加速赔偿措施的一个加速不敏感性方法这里被介绍。结果显示由利用这个方法,相等的压力产量适用由当结构的敏感从 28 fm/Pa 减少到 20 fm/Pa 时,然而,回声频率从 6.2 kHz 稍微增加到 6.5 kHz。改进动态频率特征的增加的回声频率帮助,并且当结构的敏感被减少时,它能被补偿由改进光 FBG 动态波长讯问敏感。光 FBG 水中听音器的系统敏感被它的波长讯问敏感和并非探查结构敏感主要决定。因此,加速赔偿方法将有宽广实际应用。 | WANG Junjie DING Tengjiao JIANG Desheng XIE Guanmo HUANG Junbin | 2011 | Chinese Journal of Acoustics2011，30，2： | 1 |
| 11 | Analysis of DNA methylation related to rice adult plant resistance to bacterial blight based on methylation-sensitive AFLP (MSAP) analysis显示文摘 | Sha Aihua Lin Xinhua Huang Junbin | 2005 | Molecular Genetics and Genomics2005，273，6： | 1 |
| 12 | CdS magic-size clusters exhibiting one sharp ultraviolet absorption singlet peaking at 361 nm显示文摘We report,for the first time,the synthesis of CdS magic-size clusters (MSCs) which exhibit a single sharp absorption peaking at ~ 361 nm,along with sharp band edge photoemission at ~ 377 nm and broad trap emission peaking at ~ 490 nm.These MSCs are produced in a singleensemble form without the contamination of conventional quantum dots (QDs) and/or other-bandgap clusters.They are denoted as MSC-361.We present the details of several controlled syntheses done in oleylamine (OLA),using C,d(NO3)2 or C,d(OAc)2 as a C,d source and thioacetamide (TAA) or elementary sulfur (S) as a S source.A high synthetic reproducibility of the reaction of Cd(NO3)2 and TAA to single-ensemble MSC-361 is achieved,the product of which is not contaminated by other bandgap clusters and/or QDs.In some cases,the reaction product exhibits an additional absorption peak at ~ 322 nm.We demonstrate that the two peaks,at 361 and 322 nm,do not evolve synchronously.Therefore,the 322 nm peak is not a higher order electronic transition of MSC-361,but due to the presence of another ensemble,namely MSC-322.The present study suggests that there is an outstanding need for the development of a physical model to narrow the knowledge gap regarding the electronic structure in these colloidal semiconductor CdS MSCs. | Junbin Tang Juan Hui Meng Zhang Hongsong Fan Nelson Rowell Wen Huang Yingnan Jiang Xiaoqin Chen Kui Yu | 2019 | Nano Research2019，12，6： | 1 |
| 13 | Dyskinesia is Closely Associated with Synchronization of Theta Oscillatory Activity Between the Substantia Nigra Pars Reticulata and Motor Cortex in the Off L-dopa State in Rats显示文摘Excessive theta(θ)frequency oscillation and synchronization in the basal ganglia(BG)has been reported in elderly parkinsonian patients and animal models of levodopa(L-dopa)-induced dyskinesia(LID),particularly theθoscillation recorded during periods when L-dopa is withdrawn(the off L-dopa state).To gain insight into processes underlying this activity,we explored the relationship between primary motor cortex(M1)oscillatory activity and BG output in LID.We recorded local field potentials in the substantia nigra pars reticulata(SNr)and M1 of awake,inattentive resting rats before and after L-dopa priming in Sham control,Parkinson disease model,and LID model groups.We found that chronic L-dopa increasedθsynchronization and information flow between the SNr and M1 in off L-dopa state LID rats,with a SNr-to-M1 flow directionality.Compared with the on state,θoscillational activity(θsynchronization and informationflow)during the off state were more closely associated with abnormal involuntary movements.Our findings indicate thatθoscillation in M1 may be consequent to abnormal synchronous discharges in the BG and support the notion that M1θoscillation may participate in the induction of dyskinesia. | Jiazhi Chen Qiang Wang Nanxiang Li Shujie Huang Min Li Junbin Cai Yuzheng Wang Huantao Wen Siyuan Lv Ning Wang Jinyan Wang Fei Luo Wangming Zhang | 2021 | Neuroscience Bulletin2021，37，3： | 1 |
| 14 | Ustilaginoidea virens modulates lysine 2-hydroxyisobutyrylation in rice flowers during infection显示文摘The post-translational modification lysine 2-hydroxyisobutyrylation(K_(hib))plays an important role in gene transcription,metabolism,and enzymatic activity.K_(hib)sites have been identified in rice(Oryza sativa).However,the K_(hib)status of proteins in rice flowers during pathogen infection remains unclear.Here,we report a comprehensive identification of K_(hib)-modified proteins in rice flowers,and the changes in these proteins during infection with the fungal pathogen Ustilaginoidea virens.By using a tandem mass tag-based quantitative proteomics approach,we identified 2,891 K_(hib)sites on 964 proteins in rice flowers.Our data demonstrated that 2-hydroxyisobutyrylated proteins are involved in diverse biological processes.K_(hib)levels were substantially reduced upon infection with U.virens.Chromatin immunoprecipitation polymerase chain reaction(PCR)and reverse transcription quantitative PCR analyses revealed that histone K_(hib)is involved in the expression of disease-resistance genes.More importantly,most quantified sites on core histones H3 were downregulated upon U.virens infection.In addition,the histone deacetylases HDA705,HDA716,SRT1,and SRT2 are involved in the removal of K_(hib)marks in rice.HDA705 was further confirmed to negatively regulate rice disease resistance to pathogens U.virens,Magnaporthe oryzae,and Xanthomonas oryzae pv.oryzae(Xoo).Our data suggest that U.virens could modulate K_(hib)in rice flowers during infection. | Xiaoyang Chen Qiutao Xu Yuhang Duan Hao Liu Xiaolin Chen Junbin Huang Chaoxi Luo Dao-Xiu Zhou Lu Zheng | 2021 | Journal of Integrative Plant Biology2021，63，10： | 1 |
| 15 | Variation of phytoplankton biomass and primary production in Daya Bay during spring and summer显示文摘 | Xingyu Song Liangmin Huang Jianlin Zhang Xiaoping Huang Junbin Zhang Jianqiang Yin Yehui Tan Sheng Liu | 2004 | Marine Pollution Bulletin2004，，11： | 1 |
| 16 | Larval identification of lutjanus bloch in nansha coral reefs by AFLP molecular method显示文摘 | Zhang Junbin Huang Liangmin Huo Heqiang | 2004 | Journal of experimental marine biology and ecology2004，298，1： | 1 |
| 17 | Bladder microenvironment actuated proteomotors with ammonia amplification for enhanced cancer treatment显示文摘Enzyme-driven micro/nanomotors consuming in situ chemical fuels have attracted lots of attention for biomedical applications.However,motor systems composed by organism-derived organics that maximize the therapeutic efficacy of enzymatic products remain challenging.Herein,swimming proteomotors based on biocompatible urease and human serum albumin are constructed for enhanced antitumor therapy via active motion and ammonia amplification.By decomposing urea into carbon dioxide and ammonia,the designed proteomotors are endowed with self-propulsive capability,which leads to improved internalization and enhanced penetration in vitro.As a glutamine synthetase inhibitor,the loaded L-methionine sulfoximine further prevents the conversion of toxic ammonia into non-toxic glutamine in both tumor and stromal cells,resulting in local ammonia amplification.After intravesical instillation,the proteomotors achieve longer bladder retention and thus significantly inhibit the growth of orthotopic bladder tumor in vivo without adverse effects.We envision that the as-developed swimming proteomotors with amplification of the product toxicity may be a potential platform for active cancer treatment. | Hao Tian Juanfeng Ou Yong Wang Jia Sun Junbin Gao Yicheng Ye Ruotian Zhang Bin Chen Fei Wang Weichang Huang Huaan Li Lu Liu Chuxiao Shao Zhili Xu Fei Peng Yingfeng Tu | 2023 | Acta Pharmaceutica Sinica B2023，13，9： | 0 |
| 18 | Comparative oxidation proteomics analyses suggest redox regulation of cytosolic translation in rice leaves upon Magnaporthe oryzae infection显示文摘Pathogen attack can increase plant levels of reactive oxygen species(ROS),which act as signaling molecules to activate plant defense mechanisms.Elucidating these processes is crucial for understanding redox signaling pathways in plant defense responses.Using an iodo-tandem mass tag(TMT)-based quantitative proteomics approach,we mapped 3362 oxidized cysteine sites in 2275 proteins in rice leaves.Oxidized proteins were involved in gene expression,peptide biosynthetic processes,stress responses,ROS metabolic processes,and translation pathways.Magnaporthe oryzae infection led to increased oxidative modification levels of 512 cysteine sites in 438 proteins,including many transcriptional regulators and ribosomal proteins.Ribosome profiling(Ribo-seq)analysis revealed that the oxidative modification of ribosomal proteins promoted the translational efficiency of many mRNAs involved in defense response pathways,thereby affecting rice immunity.Our results suggest that increased oxidative modification of ribosomal proteins in rice leaves promotes cytosolic translation,thus revealing a novel function of posttranslational modifications.Furthermore,the oxidation-sensitive proteins identified here provide a valuable resource for research on protein redox regulation and can guide future mechanistic studies. | Xiaoyang Chen Qiutao Xu Yaping Yue Yuhang Duan Hao Liu Xiaolin Chen Junbin Huang Lu Zheng | 2023 | Plant Communications2023，4，3： | 0 |
| 19 | The co-removal of PCDD/Fs by SCR system in a full-scale municipal solid waste incinerator:Migration-transformation and decomposition pathways显示文摘Polychlorinated dibenzo-p-dioxins and furans(PCDD/Fs)generated from municipal solid waste(MSW)incineration are considered as the key pollutants,which could pose serious risks to the environment and ecology.This study comprehensively investigated the effect of selective catalytic reduction(SCR)system on PCDD/F removal,phase distributions,and migrationtransformation characteristics of 17 congeners in a full-scale MSW incinerator.The three flue gas samples and two fly ash samples were separately collected for PCDD/F analysis.The results showed that the SCR system reduced the I-TEQ concentration of PCDD/Fs from 0.135 to 0.010 ng I-TEQ Nm^(−3)with a remarkable toxicity removal efficiency of 92.6%,through oxidation decomposition over V_(2)O_(5)-WO_(3)/TiO_(2)catalysts and chlorination process of low-chlorinated congeners.In addition,the de novo synthesis observed along the flue between SCR system and stack regenerated unexpected PCDD/Fs,which might enhance the PCDD/F emission level.This study verified the three pathways of SCR system on PCDD/F removal and inspired operating suggestions for SCR system,i.e.,keeping SCR system operated in stable and consistent conditions,regularly replacing V_(2)O_(5)-WO_(3)/TiO_(2)catalysts,and timely cleaning the dusts and inlet materials of SCR system,which would be beneficial for achieving the ultra-low emission of PCDD/Fs in full-scale MSW incinerators. | WANG PengJu YAN Feng XIE Feng CAI JianJun SHEN XueHua WEI XuanKun HUANG JunBin LI ZhongZheng ZHONG RiGang ZHANG ZuoTai | 2022 | Science China(Technological Sciences)2022，65，10： | 0 |
| 20 | A lipid droplet-associated protein Nem1 regulates appressorium function for infection of Magnaporthe oryzae显示文摘Lipid droplets are important storages in fungal conidia and can be used by plant pathogenic fungi for infection.However,the regulatory mechanism of lipid droplets formation and the utilization during fungal development and infection are largely unknown.Here,in Magnaporthe oryzae,we identified a lipid droplet-associated protein Nem1 that played a key role in lipid droplets biogenesis and utilization.Nem1 was highly expressed in conidia,but lowly expressed in appressoria,and its encoded protein was localized to lipid droplets.Deletion of NEM1 resulted in reduced numbers of lipid droplets and decreased content of diacylglycerol(DAG)or triacylglycerol(TAG).NEM1 was required for asexual development especially conidia production.TheΔnem1 mutant was nearly loss of virulence to host plants due to defects in appressorial penetration and invasive growth.Remarkably,Nem1 was regulated by the TOR signaling pathway and involved in the autophagy process.The Ser303 residue of Nem1 could be phosphorylated by the cAMP-PKA signaling pathway and was important for biological function of Nem1.Together,our study revealed a regulatory mechanism of lipid biogenesis and metabolism during the conidium and appressorium formation of the rice blast fungus. | Deng Chen Xuan Cai Junjie Xing Shen Chen Juan Zhao Zhiguang Qu Guotian Li Hao Liu Lu Zheng Junbin Huang Xiao-Lin Chen | 2023 | aBIOTECH2023，4，2： | 0 |
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