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| 1 | Cationic nanocarriers induce cell necrosis through impairment of Na^+/K^+-ATPase and cause subsequent inflammatory response显示文摘有积极表面费用的 Nanocarriers 以他们限制了他们的临床的应用的毒性著名。位于他们的毒性下面的机制例如煽动性的反应的正式就职,仍然保持大部分未知。在我们发现了 cationic nanocarriers 的那注射的现在的学习,包括 cationic liposomes, PEI,和 chitosan,导致了坏死的房间的快速的外观。cationic nanocarriers 导致的房间坏死依赖于他们的积极表面费用,但是不要求 RIP1 和 Mlkl。相反,细胞内部的 Na + 超载被发现伴随房间死亡。在文化媒介或有 Na +/K+-ATPase 阳离子绑定地点禁止者 ouabain 的房间的预告的处理的 Na + 的弄空,从房间坏死的保护的房间。而且,有 cationic nanocarriers 的处理在 vitro 并且在 vivo 禁止了 Na +/K+-ATPase 活动。计算模拟证明 cationic 搬运人能与 Na +/K+-ATPase 的阳离子绑定地点交往。有 ouabain 的小剂量的老鼠 pretreated 在 cationic nanocarriers 的致命的剂量的注射以后显示出改进幸存。进一步的分析建议 cationic nanocarriers 和 mitochondrial DNA 的产生的漏导致的那房间坏死能在 vivo 触发严重发炎,它被包含 TLR9 并且 MyD88 发信号的一条小径调停。一起拿,我们的结果揭示由此的新奇机制 cationic nanocarriers 通过和 mitochondrial 的随后的暴露的和 Na +/K+-ATPase, 的相互作用导致尖锐房间坏死是的联系损坏的分子的模式调停的一个关键事件煽动性的回答。我们的学习为评估 nanocarriers 的 biocompatibility 并且为药交货设计更好、更安全的有重要含意。 | Xiawei Wei Bin Shao Zhiyao He Tinghong Ye Min Luo Yaxiong Sang Xiao Liang Wei Wang Shuntao Luo Shengyong Yang Shuang Zhang Changyang Gong Maling Gou Hongxing Deng Yinglan Zhao Hanshuo Yang Senyi Deng Chengjian Zhao Li Yang Zhiyong Qian Jiong Li Xun Sun Jiahuai Han Chengyu Jiang Min Wu Zhirong Zhang | 2015 | Cell Research2015，25，2： | 21 |
| 2 | Histones released by NETosis enhance the infectivity of SARS-CoV-2 by bridging the spike protein subunit 2 and sialic acid on host cells显示文摘Neutrophil extracellular traps(NETs)can capture and kill viruses,such as influenza viruses,human immunodeficiency virus(HIV),and respiratory syncytial virus(RSV),thus contributing to host defense.Contrary to our expectation,we show here that the histones released by NETosis enhance the infectivity of SARS-CoV-2,as found by using live SARS-CoV-2 and two pseudovirus systems as well as a mouse model.The histone H3 or H4 selectively binds to subunit 2 of the spike(S)protein,as shown by a biochemical binding assay,surface plasmon resonance and binding energy calculation as well as the construction of a mutant S protein by replacing four acidic amino acids.Sialic acid on the host cell surface is the key molecule to which histones bridge subunit 2 of the S protein.Moreover,histones enhance cell-cell fusion.Finally,treatment with an inhibitor of NETosis,histone H3 or H4,or sialic acid notably affected the levels of sgRNA copies and the number of apoptotic cells in a mouse model.These findings suggest that SARS-CoV-2 could hijack histones from neutrophil NETosis to promote its host cell attachment and entry process and may be important in exploring pathogenesis and possible strategies to develop new effective therapies for COVID-19. | Weiqi Hong Jingyun Yang Jun Zou Zhenfei Bi Cai He Hong Lei Xuemei He Xue Li Aqu Alu Wenyan Ren Zeng Wang Xiaohua Jiang Kunhong Zhong Guowen Jia Yun Yang Wenhai Yu Qing Huang Mengli Yang Yanan Zhou Yuan Zhao Dexuan Kuang Junbin Wang Haixuan Wang Siyuan Chen Min Luo Ziqi Zhang Tiangi Lu Li Chen Haiying Que Zhiyao He Qiu Sun Wei Wang Guobo Shen Guangwen Lu Zhiwei Zhao Li Yang Jinliang Yang Zhenling Wang Jiong Li Xiangrong Song Lunzhi Dai Chong Chen Jia Geng Maling Gou Lu Chen Haohao Dong Yong Peng Canhua Huang Zhiyong Qian Wei Cheng Changfa Fan Yuquan Wei Zhaoming Su Aiping Tong Shuaiyao Lu Xiaozhong Peng Xiawei Wei | 2022 | Cellular & Molecular Immunology2022，19，5： | 2 |
| 3 | Synthesis and characterization of PEG-PCL-PEG thermosensitive hydrogel显示文摘 | ChangYang Gong Shuai Shi PengWei Dong Bing Kan MaLing Gou XianHuo Wang XingYi Li Feng Luo Xia Zhao YuQuan Wei ZhiYong Qian | 2008 | International Journal of Pharmaceutics2008，，1： | 1 |
| 4 | Self-assembled honokiol-loaded micelles based on poly(?-caprolactone)-poly(ethylene glycol)-poly(?-caprolactone) copolymer显示文摘 | XiaWei Wei ChangYang Gong Shuai Shi ShaoZhi Fu Ke Men Shi Zeng XiuLing Zheng MaLing Gou LiJuan Chen LiYan Qiu ZhiYong Qian | 2008 | International Journal of Pharmaceutics2008，，1： | 1 |
| 5 | A novel injectable local hydrophobic drug delivery system: Biodegradable nanoparticles in thermo-sensitive hydrogel显示文摘 | MaLing Gou XingYi Li Mei Dai ChangYang Gong XianHuo Wang Yao Xie HongXin Deng LiJuan Chen Xia Zhao ZhiYong Qian YuQuan Wei | 2008 | International Journal of Pharmaceutics2008，，1： | 1 |
| 6 | Biodegradable poly ( epsilon- caprolactone) - poly ( ethylene glycol ) eopoly- mers as drug deliverysystem 显示文摘 | XiaWei Wei ChangYang Gong MaLing Gou | 2009 | International Journal of Phar- maceutics2009，381，1： | 1 |
| 7 | Biodegradable poly (e-caprolaction)-poly (ethylene glycol ) copolymers as drug delivery system 显示文摘 | Wei Xiawei Gong Changyang Gou Maling | 2009 | International Journal of Pharmaceutics2009，38，： | 1 |
| 8 | In Vitro Degradation Behavior of Polyesteramide Copolymer Fiber Based on 6-Aminocaproic Acid, Adipic Acid, and 1,6-Hexane Diol显示文摘 | CaiBing Liu WenJuan Jia ZhiYong Qian MeiJuan Huang YingChun Gu GuoTao Chao MaLing Gou ChangYang Gong HongXin Deng Ke Lei AnLiang Huang MingJing Tu | 2007 | Journal of Polymer Research2007，，1： | 1 |
| 9 | Strategies for improving adipose-derived stem cells for tissue regeneration显示文摘Adipose-derived stem cells(ADSCs)have promising applications in tissue regeneration.Currently,there are only a few ADSC products that have been approved for clinical use.The clinical application of ADSCs still faces many challenges.Here,we review emerging strategies to improve the therapeutic efficacy of ADSCs in tissue regeneration.First,a great quantity of cells is often needed for the stem cell therapies,which requires the advanced cell expansion technologies.In addition cell-derived products are also required for the development of‘cell-free’therapies to overcome the drawbacks of cell-based therapies.Second,it is necessary to strengthen the regenerative functions of ADSCs,including viability,differentiation and paracrine ability,for the tissue repair and regeneration required for different physiological and pathophysiological conditions.Third,poor delivery efficiency also restricts the therapeutic effect of ADSCs.Effective methods to improve cell delivery include alleviating harsh microenvironments,enhancing targeting ability and prolonging cell retention.Moreover,we also point out some critical issues about the sources,effectiveness and safety of ADSCs.With these advanced strategies to improve the therapeutic efficacy of ADSCs,ADSC-based treatment holds great promise for clinical applications in tissue regeneration. | Xin Yuan Li Li Haofan Liu Jing Luo Yongchao Zhao Cheng Pan Xue Zhang Yuwen Chen Maling Gou | 2022 | Burns & Trauma2022，10，1： | 1 |
| 10 | Biode- gradable poly ( ε -caprolactone) -poly (ethylene glycol) copolymers as drug delivery system 显示文摘 | Wei Xiawei Gong Changyang Gou Maling | 2009 | International Journal of Phamaceutics2009，381，1： | 1 |
| 11 | Synthesis and characterization of PEG-PCL-PEG thermosensitive hydrogel显示文摘 | ChangYang Gong Shuai Shi PengWei Dong Bing Kan MaLing Gou XianHuo Wang XingYi Li Feng Luo Xia Zhao YuQuan Wei ZhiYong Qian | 2008 | International Journal of Pharmaceutics2008，，1： | 1 |
| 12 | Nerve transfer with 3D-printed branch nerve conduits显示文摘Background:Nerve transfer is an important clinical surgical procedure for nerve repair by the coaptation of a healthy donor nerve to an injured nerve.Usually,nerve transfer is performed in an end-to-end manner,which will lead to functional loss of the donor nerve.In this study,we aimed to evaluate the efficacy of 3D-printed branch nerve conduits in nerve transfer.Methods:Customized branch conduits were constructed using gelatine-methacryloyl by 3D print-ing.The nerve conduits were characterized both in vitro and in vivo.The efficacy of 3D-printed branch nerve conduits in nerve transfer was evaluated in rats through electrophysiology testing and histological evaluation.Results:The results obtained showed that a single nerve stump could form a complex nerve network in the 3D-printed multibranch conduit.A two-branch conduit was 3D printed for transferring the tibial nerve to the peroneal nerve in rats.In this process,the two branches were connected to the distal tibial nerve and peroneal nerve.It was found that the two nerves were successfully repaired with functional recovery.Conclusions:It is implied that the two-branch conduit could not only repair the peroneal nerve but also preserve partial function of the donor tibial nerve.This work demonstrated that 3D-printed branch nerve conduits provide a potential method for nerve transfer. | Jing Zhang Jie Tao Hao Cheng Haofan Liu Wenbi Wu Yinchu Dong Xuesong Liu Maling Gou Siming Yang Jianguo Xu | 2022 | Burns & Trauma2022，10，1： | 0 |
| 13 | 3D printing of functional nerve guide conduits显示文摘Nerve guide conduits(NGCs),as alternatives to nerve autografts and allografts,have been widely explored as an advanced tool for the treatment of peripheral nerve injury.However,the repairing efficiency of NGCs still needs significant improvements.Functional NGCs that provide a more favorable microenvironment for promoting axonal elongation and myelination are of great importance.In recent years,3D printing technologies have been widely applied in the fabrication of customized and complex constructs,exhibiting great potential for tissue engineering applications,especially for the construction of functional NGCs.In this review,we introduce the 3D printing technologies for manufacturing functional NGCs,including inkjet printing,extrusion printing,stereolithographybased printing and indirect printing.Further,we summarize the current methods and strategies for constructing functional NGCs,such as designing special conduit architectures,using appropriate materials and co-printing with different biological cues.Finally,the challenges and prospects for construction of functional NGCs are also presented. | Yulan Huang Wenbi Wu Haofan Liu Yuwen Chen Bo Li Zhiyuan Gou Xun Li Maling Gou | 2021 | Burns & Trauma2021，9，1： | 0 |
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