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| 1 | Identification of a Stable Quantitative Trait Locus for Percentage Grains with White Chalkiness in Rice (Oryza sativa)显示文摘High chalkiness is a major problem in many rice-producing areas of the world, especially in hybrid rice (Oryza sativa L.) in China. We previously showed a major quantitative trait locus for the percentage of grains with white chalkiness (QTLqPGWC-8) in the interval G1149-R727 on chromosome 8 using a chromosome segment substitution line (CSSL). Here, we selected the line-CSSL50 harboring the QTLqPGWC-8 allele from the CSSLs derived from a cross between Asominori (as a recurrent parent) and IR24 (as a donor parent), which had higher percentage chalkiness, markedly different from that of Asominori. There were also significant differences in starch granules, appearance of amylose content (AAC) and milling qualities between Asominori and CSSL50, but not in grain size or thousand grain weight (TGW). The BC4F2 and BC4F3 populations from a cross between CSSL50 and Asominori were used for fine mapping of qPGWC-8. We narrowed down the location of this QTL to a 142 kb region between Indel markers 8G-7 and 8G-9. QTLqPGWC-8 accounted for 50.9% of the difference in PGWC between the parents. The markers tightly linked to qPGWC-8 should facilitate cloning of the gene underlying this QTL and will be of value for marker-assisted selection in breeding rice varieties with better grain quality. | Tao Guo Xiaolu Liu Xiangyuan Wan Jianfeng Weng Shijia Liu Xi Liu Mingjiang Chen Jingjing Li Ning Su Fuqing Wu Zhijun Cheng Xiuping Guo Cailin Lei Jiulin Wang Ling jiang Jianmin Wan | 2011 | Journal of Integrative Plant Biology2011，53，8： | 25 |
| 2 | A cyclic nucleotide-gated channel mediates cytoplasmic calcium elevation and disease resistance in rice显示文摘The transient elevation of cytoplasmic calcium is essential for pathogen-associated molecular pattern(PAMP)-triggered immunity(PTI).However,the calcium channels responsible for this process have remained unknown.Here,we show that rice CDSI(CELL DEATH and SUSCEPTIBLE to BLAST 1)encoding OsCNGC9;a cyclic nucleotide-gated channel protein,positively regulates the resistance to rice blast disease.We show that OsCNGC9 mediates PAMP-induced Ca2+influx and that this event is critical for PAMPs-triggered ROS burst and induction of PTI-related defense gene expression.We further show that a PTI related receptor-lih cytoplasmic kinase OsRLCK185 physically interacts with and phosphorylates OsCNGC9 to activate its channel activity.Our results suggest a signaling cascade linking pattern recognition to calcium channel activation,which is required for initiation of PTI and disease resistance in rice. | Jiachang Wang Xi Liu An Zhang Yulong Ren Fuqing Wu Gang Wang Yang Xu Cailin Lei Shanshan Zhu Tian Pan Yongfei Wang Huan Zhang Fan Wang Yan-Qiu Tan Yupeng Wang Xin Jin Sheng Luo Chunlei Zhou Xiao Zhang Jinling Liu Shuai Wang Lingzhi Meng Yihua Wang Xi Chen Qibing Lin Xin Zhang Xiuping Guo Zhijun Cheng Jiulin Wang Yunlu Tian Shijia Liu Ling Jiang Chuanyin Wu Ertao Wang Jian-Min Zhou Yong-Fei Wang Haiyang Wang Jianmin Wan | 2019 | Cell Research2019，29，10： | 13 |
| 3 | The Influence of Extractable Organic Matter on Pore Development in the Late Triassic Chang 7 Lacustrine Shales, Yanchang Formation, Ordos Basin, China显示文摘To investigate the influence of extractable organic matter(EOM) on pore evolution of lacustrine shales, Soxhlet extraction, using dichloromethane, was performed on a series of Chang 7 shale samples(Ordos Basin, China) with vitrinite reflectance of 0.64% to 1.34%. Low-pressure gas adsorption experiments were conducted on the samples before and after extraction. The pore structure parameters were calculated from the gas adsorption data. The results show complex changes to the pore volumes and surface areas after extraction. The pore development of both the initial and extracted samples is strongly controlled by total organic carbon(TOC) content. Micropores developed mainly in organic matter(OM), while mesopores and macropores predominantly developed in fractions other than OM. The influence of EOM on micropores is stronger than on mesopores and macropores. Organic solvents with a higher boiling point should be used to explore the effect of EOM on pore structure in the future. | HAN Hui LIU Pengwei DING Zhengang SHI Pitong JIA Jianchao ZHANG Wei LIU Yan CHEN Shijia LU Jungang Chen Kang PENG Xudong WANG Zhiyong XIAO Shuqi GAO Yuans | 2018 | Acta Geologica Sinica(English Edition)2018，92，4： | 8 |
| 4 | Realization of warm white light emitting in single phase Gd(P_(x)V_(1−x))O_(4):y at% Sm^(3+),1 at% Bi^(3+) phosphor显示文摘A series of single phase,warm white light emitting phosphors,Gd(P_(x)V_(1−x))O_(4):y at%Sm^(3+),with 1 at%Bi^(3+) doping concentration were synthesized by high temperature solid state method in this work.The experimental results indicate broadband cyan emission of Bi^(3+)and characteristic orange-red emission of Sm^(3+)can be effectively tuned by changing the ratios of PO^(3−)_(4)/VO^(3−)_(4) in Gd(P_(x)V_(1−x))O_(4):1 at%Sm^(3+),1 at%Bi^(3+),and the energy transfer process among VO^(3−)_(4),Sm^(3+),Bi^(3+) also can be adjusted.Based on this,warm white light emitting can be realized by further optimizing the doping concentration of Sm^(3+) in the phosphors.At 423 K,the PL intensity of Gd(P_(0.7)V_(0.3))O_(4):2 at%Sm^(3+),1 at%Bi^(3+) remains~84.3%of the initial value at 293 K,while the measured quantum efficiency is 67.8%.EL spectrum analysis results of the fabricated white light emitting diode(wLED)based on a 310 nm UV-chip and Gd(P_(0.7)V_(0.3))O4:2 at%Sm^(3+),1 at%Bi^(3+)phosphors imply low correlated color temperature(3132 K)and appropriate color-rending index(R_(a)=82.7).These results demonstrate that Gd(P_(0.7)V_(0.3))O4:2 at%Sm^(3+),1 at%Bi^(3+)is a good candidate for manufacturing UV-activated warm white light emitting diodes. | Chao Dou Yanzhen Yin Fei Zheng Zhen Wang Shijia Sun Chen Hu Yang Che Bing Teng Degao Zhong | 2022 | Journal of Rare Earths2022，40，4： | 2 |
| 5 | Selection, identification, and application of Aflatoxin B1 aptamer显示文摘 | Xiaoyuan Ma Wenfeng Wang Xiujuan Chen Yu Xia Shijia Wu Nuo Duan Zhouping Wang | 2014 | European Food Research and Technology2014，，6： | 1 |
| 6 | Ribonuclease H-like gene SMALL GRAIN2 regulates grain size in rice through brassinosteroid signaling pathway显示文摘Grain size is a key agronomic trait that determines the yield in plants.Regulation of grain size by brassinosteroids(BRs)in rice has been widely reported.However,the relationship between the BR signaling pathway and grain size still requires further study.Here,we isolated a rice mutant,named small grain2(sg2),which displayed smaller grain and a semi-dwarf phenotype.The decreased grain size was caused by repressed cell expansion in spikelet hulls of the sg2 mutant.Using map-based cloning combined with a Mut Map approach,we cloned SG2,which encodes a plant-specific protein with a ribonuclease H-like domain.SG2 is a positive regulator downstream of GLYCOGEN SYNTHASE KINASE2(GSK2)in response to BR signaling,and its mutation causes insensitivity to exogenous BR treatment.Genetical and biochemical analysis showed that GSK2 interacts with and phosphorylates SG2.We further found that BRs enhance the accumulation of SG2 in the nucleus,and subcellular distribution of SG2 is regulated by GSK2 kinase activity.In addition,Oryza sativa OVATE family protein 19(OsOFP19),a negative regulator of grain shape,interacts with SG2 and plays an antagonistic role with SG2 in controlling gene expression and grain size.Our results indicated that SG2 is a new component of GSK2-related BR signaling response and regulates grain size by interacting with Os OFP19. | Yunshuai Huang Hui Dong Changling Mou Ping Wang Qixian Hao Min Zhang Hongmin Wu Fulin Zhang Tengfei Ma Rong Miao Kai Fu Yaping Chen Ziyan Zhu Cheng Chen Qikai Tong Zhuoran Wang Shirong Zhou Xi Liu Shijia Liu Yunlu Tian Ling Jiang Jianmin Wan | 2022 | Journal of Integrative Plant Biology2022，64，10： | 1 |
| 7 | Mesoscale simulation of the high-temperature austenitizing and dynamic recrystallization by coupling a cellular automaton with a topology deformation technique显示文摘 | Fei Chen Zhenshan Cui Juan Liu Wen Chen Shijia Chen | 2010 | Materials Science & Engineering A2010，，21： | 1 |
| 8 | A dual-color flow cytometry protocol for the simultaneous detection of Vibrio parahaemolyticus and Salmonella typhimurium using aptamer conjugated quantum dots as labels显示文摘 | Nuo Duan Shijia Wu Ye Yu Xiaoyuan Ma Yu Xia Xiujuan Chen Yukun Huang Zhouping Wang | 2013 | Analytica Chimica Acta2013，，： | 1 |
| 9 | Analysis of isorhamnetin-3-O-neohesperidoside in rat plasma by liquid chro- matography/electrospray ionization tandem mass spectrometry and its application to pharmacokinetic studies 显示文摘 | Liu Shijia Chen Peidong Dai Guoliang | 2013 | Chin J Nat Med2013，11，5： | 1 |
| 10 | Accumulation Pattern of Jurassic Tight Oil in Sichuan Basin and Its Exploration & Development Prospect显示文摘Jurassic tight oil in Sichuan Basin is chiefly distributed over the central basin with an area of about 4.2×104 km2.Since the first drilling in 1953,it has experienced four stages,including the Central Sichuan Basin Brought under Exploration and Development,Progressive Exploration and Development,Setting A Goal of 30×104 t/a,and Adjusting and Keeping A Stable Production.More than sixty-year exploration and development | LI Yaohua YANG Guang TAO Shizhen NI Chao CHEN Shijia LI Zhou | 2015 | Acta Geologica Sinica(English Edition)2015，89，A01： | 1 |
| 11 | CsWRKY13,a novel WRKY transcription factor of Camellia sinensis,involved in lignin biosynthesis and accumulation显示文摘Lignin is an aromatic polymer that provides the necessary mechanical strength for the transport of water and nutrients in higher plants.Lignin biosynthesis and accumulation affect growth and development of tea plants.The degree of lignification related to the tenderness of fresh tea leaves determines the quality of tea.WRKY transcription factors play central roles in plant development and physiological processes.However,the roles of WRKY transcription factors in lignin biosynthesis of tea plants remain unclear.In this study,a WRKY gene,CsWRKY13,was cloned from tea plant'Longjing 43'.The open reading frame(ORF)of CsWRKY13 gene was 708 bp,encoding 235 amino acids.Sequence analysis showed that CsWRKY13 contained a conserved WRKYGQK amino acid sequence and a zinc-finger-like motif CX4CX23HXH.Subcellular localization showed that CsWRKY13 was localized in the nucleus.The yeast trans-activation assay showed that CsWRKY13 had no transcriptional activity.Expression analysis showed that the CsWRKY13 gene was highly expressed in the stem.Overexpression of CsWRKY13 in Arabidopsis thaliana reduced lignin content and the expression levels of genes related to lignin biosynthesis in transgenic plants.Most flavonoids pathway related genes were significantly up-regulated.This study shows that CsWRKY13 might function as a negative regulator in regulation of lignin synthesis. | Ruimin Teng Yongxin Wang Shijia Lin Yi Chen Yazhuo Yang Ni Yang Jingwen Li Jing Zhuang | 2021 | Beverage Plant Research2021，1，1： | 1 |
| 12 | Fine mapping of S32(t), a new gene causing hybrid embryo sac sterility in a Chinese landrace rice (Oryza sativa L.)显示文摘 | Danting Li Liangming Chen Ling Jiang Susong Zhu Zhigang Zhao Shijia Liu Ning Su Huqu Zhai Hiroshi Ikehashi Jianmin Wan | 2007 | Theoretical and Applied Genetics2007，，3： | 1 |
| 13 | Geochemical Characteristics and Origin of Crude Oil and Natural Gas in the Southern Slope Zone,Kuqa Foreland Basin,NW China显示文摘Continuous exploration has triggered a heated debate on hydrocarbon resource potential in the southern slope zone of the Kuqa foreland basin,and sources of the Mesozoic-Cenozoic oil and gas have become a key problem to be solved in this region.Composition and organic geochemical parameters of crude oil and natural gas from the southern slope zone of the Kuqa foreland basin were illustrated in order to reveal their origin by using a combination of gas chromatograph(GC),gas chromatogram-mass spectrum(GC-MS)and carbon isotope analyses.The characteristics of crude oil,such as low density,viscosity,solidification point and sulfur content,and high wax content,indicate that source of the crude oil is continental.The biomarker compositions of crude oil are characterized by low to medium molecular weight compounds(n-C_(12) to n-C_(20)),high Pr/Ph ratios(>1.0),low phytane/n-C18 ratios(0.06-0.54),and predominant regular sterane C_(29).All biomarker parameters clearly indicate that the crude oil was derived mainly from algae and aquatic plankton and deposited under weak reduction-oxidation environment,and has the characteristics of mixed kerogens.The Cretaceous crude oil was mainly derived from the Triassic lacustrine source rocks,which also contributed to the Paleogene crude oil together with Jurassic coal source rocks.Natural gas is characterized by moderate methane content,high heavy hydrocarbon and nitrogen content,and no hydrogen sulfide.The methane and ethane in Paleogene natural gas are relatively rich in ^(13)C withδ^(13)C_(1) andδ^(13)C_(2) values ranging from-37.3‰to-31.2‰(mean=-34.25‰)and from-25‰to-21.3‰(mean=-23.09‰),respectively,indicating the coal-derived gas from the Middle and Lower Jurassic strata.Hydrocarbon products in the southern slope zone of the Kuqa foreland basin are primarily generated from source rocks in the mature stage.The low-amplitude structural and lithologic traps with the updip pinch-out sand bodies or plugging secondary fault at relatively high tectonic positions are the most favorable areas for discovery and breakthrough in the study area.Results of this study will provide useful information for controlling factors of reservoirs and oil and gas exploration deployment in the southern slope zone of the Kuqa foreland basin. | Chun Liu Shijia Chen Jilong Zhao Zhou Su Hui Rong | 2022 | Journal of Earth Science2022，33，3： | 1 |
| 14 | Genetic dissection of top three leaf traits in rice using progenies from a japonica × indica cross显示文摘The size of the top three leaves of rice plants is strongly associated with yield; thus, it is important to consider quantitative traits representing leaf size(e.g.,length and width) when breeding novel rice varieties.It is challenging to measure such traits on a large scale in the field, and little is known about the genetic factors that determine the size of the top three leaves. In the present study, a population of recombinant inbred lines(RILs) and reciprocal single chromosomal segment substitution lines(SSSLs) derived from the progeny of a japonica Asominori ? indica IR24 cross were grown under four diverse environmental conditions. Six morphological traits associated with leaf size were measured,namely length and width of the flag, second and third leaves. In the RIL population, 49 QTLs were identified that clustered in 30 genomic region. Twenty-three of these QTLs were confirmed in the SSSL population. A comparison with previously reported genes/QTLs revealed eight novel genomic regions that contained uncharacterized ORFs associated with leaf size. The QTLs identified in this study can be used for markerassisted breeding and for fine mapping of novel genetic elements controlling leaf size in rice. | Changbin Yin Huihui Li Zhigang Zhao Zhiquan Wang Shijia Liu Liangming Chen Xi Liu Yunlu Tian Juan Ma Lidong Xu Dashuang Zhang Susong Zhu Danting Li Jianmin Wan Jiankang Wang | 2017 | Journal of Integrative Plant Biology2017，59，12： | 1 |
| 15 | Identification and characterization of a novel Waxy allele from a Yunnan rice landrace显示文摘 | Linglong Liu Xiaodong Ma Shijia Liu Changlan Zhu Ling Jiang Yihua Wang Yi Shen Yulong Ren Hui Dong Liangming Chen Xi Liu Zhigang Zhao Huqu Zhai Jianmin Wan | 2009 | Plant Molecular Biology2009，，6： | 1 |
| 16 | Quantitative study on hydrocarbon expulsion mechanism based on micro-fracture显示文摘The significance of source rocks for oil and gas accumulation has been indisputably acknowledged.Moreover,it has been gradually realized that there is difference between hydrocarbon generation capacity and hydrocarbon expulsion capacity,and this has prompted research on hydrocarbon expulsion efficiency.However,these studies dominantly highlight the results of hydrocarbon expulsion,and investigation into the corresponding process and mechanism is primarily from a macroscopic perspective.Despite its wide acceptance as the most direct hydrocarbon expulsion mode,hydrocarbon expulsion through micro-fractures is still not sufficiently understood.Therefore,this study obtains observations and performs experiments on two types of source rocks(mudstones and shales)of the Chang 7 oil group of the Yanchang Formation in Ordos Basin,China.Microscopy reveals that organic matter is non-uniformly distributed in both types of source rocks.Specifically,mudstones are characterized by a cluster-like organic matter distribution,whereas shales are characterized by a layered organic matter distribution.Thermal evolution simulation experiments demonstrate that the hydrocarbon generation process is accompanied by the emergence of micro-fractures,which are favorable for hydrocarbon expulsion.Moreover,based on the theories of rock physics and fracture mechanics,this study establishes micro-fracture development models for both types of source rocks,associated with the calculation of the fracture pressure that is needed for the initiation of fracture development.Furthermore,the relationship between the fluid pressure,fracture pressure,and micro-fracture expansion length during micro-fracture development is quantitatively explored,which helps identify the micro-fracture expansion length.The results indicate that the development of micro-fractures is commonly impacted by the morphology and distribution pattern of the organic matter as well as the mechanical properties of the source rocks.The micro-fractures in turn further affect the hydrocarbon expulsion capacity of the source rocks.The results of this study are expected to provide theoretical and practical guidance for the exploration and exploitation of tight oil and shale oil. | Kaiming Su Jungang Lu Huanxu Zhang Shijia Chen Yong Li Zhenglu Xiao Wen Qiu Meimei Han | 2020 | Geoscience Frontiers2020，11，6： | 1 |
| 17 | Liquid chromatograph/tandem mass spectrometry assay for the simultaneous determination of chlorogenic acid and cinnamic acid in plasma and its application to a pharmacokinetic study显示文摘 | Jun Zhang Min Chen Wenzheng Ju Shijia Liu Meijuan Xu Jihong Chu Ting Wu | 2009 | Journal of Pharmaceutical and Biomedical Analysis2009，，3： | 1 |
| 18 | A novel inhibitor of ARfl and ARv7 induces protein degradation to overcome enzalutamide resistance in advanced prostate cancer显示文摘Enzalutamide(ENZ) is a second-generation androgen receptor(AR) antagonist used for the treatment of castration-resistant prostate cancer(CRPC) and reportedly prolongs survival time within a year of starting therapy. However, CRPC patients can develop ENZ resistance(ENZR), mainly driven by abnormal reactivation of AR signaling, involving increased expression of the full-length AR(ARfl)or dominantly active androgen receptor splice variant 7(ARv7) and ARfl/ARv7 heterodimers. There is currently no efficient treatment for ENZR in CRPC. Herein, a small molecule LLU-206 was rationally designed based on the ENZ structure and exhibited potent inhibition of both ARfl and constitutively active ARv7 to inhibit PCa proliferation and suppress ENZR in CRPC. Mechanically, LLU-206 promoted ARfl/ARv7 protein degradation and decreased ARfl/ARv7 heterodimers through mouse double minute 2-mediated ubiquitination. Finally, LLU-206 exhibited favorable pharmacokinetic properties with poor permeability across the blood-brain barrier, leading to a lower prevalence of adverse effects, including seizure and neurotoxicity, than ENZ-based therapies. In a nutshell, our findings demonstrated that LLU-206 could effectively inhibit ARfl/ARv7-driven CRPC by dual-targeting of ARfl/ARv7 heterodimers and protein degradation, providing new insights for the design of new-generation AR inhibitors to overcome ARfl/ARv7-driven CRPC. | Yan Li Ya Chu Guangjiang Shi Xiaobin Wang Wanli Ye Chun Shan Dajia Wang Di Zhang Wei He Jingwei Jiang Shuqian Ma Yuhong Han Zhili Zhao Shijia Du Zhen Chen Zhiyu Li Yong Yang Chen Wang Xi Xu Hongxi Wu | 2022 | Acta Pharmaceutica Sinica B2022，12，11： | 0 |
| 19 | A topological polymer network with Cu(Ⅱ)-coordinated reversible imidazole-urea locked unit constructs an ultra-strong self-healing elastomer显示文摘It is exceedingly desired, but difficult to construct self-healing materials with both excellent mechanical properties and healing efficiency, which are usually realized by using mutually exclusive methods. Here, we reconcile this contradiction by utilizing copper-bis-(imidazole-2-yl)-methane-urea(Cu-BIMU) locked units based on novel designed dynamic imidazole-urea bonds with coupled multiple noncovalent bonds(coordination bonds, π-π stacking bonds, and hydrogen bonds). The coordination of Cu(II) greatly reduces the electron-cloud density of imidazole, which lowers the free energy barrier of imidazole-urea bonds and promotes their reversible dissociation, as demonstrated by the density functional theory and small-molecule model reaction. The topological design of Cu-BIMU polyurethane(Cu-BIMU-PU), which concentrates multiple crosslinking-in-one locked unit to avoid the formation of excessive crosslinking sites to ensure high chain mobility, facilitates self-healing. Accumulative extensive intermolecular interactions endowed excellent mechanical properties to the resulting Cu-BIMU-PU elastomer with a tensile strength of 65.3 MPa, among the highest ever-reported value. This work provides a novel molecular design principle for fabricating high-performance dynamic polymers. | Lei Yang Zenghe Liu Rasoul Esmaeely Neisiany Jiaming Lou Yifan Guo Luzhi Zhang Huijie Liu Shuo Chen Shijia Gu Zhengwei You | 2023 | Science China Chemistry2023，66，3： | 0 |
| 20 | Factors controlling the reservoir accumulation of Triassic Chang 6 Member in Jiyuan-Wuqi area,Ordos Basin,NW China显示文摘In the Triassic Yanchang Formation, Jiyuan-Wuqi area, Ordos Basin, the Chang 6 reservoir is contacted to the Chang 7 high-quality source rock, but the oil pools are unevenly distributed, and complex in oil and water distribution. Through cores observation and fracture statistics, combined with comprehensive analyses of physical property, mercury injection, logging and geochemical data, and comparisons of the sandbodies scales, reservoir physical properties, argillaceous laminae and fractures between source and reservoir in the eastern and western oil-bearing areas and in the central water producing area, it is found that the hydrocarbon accumulation patterns are different in the eastern, central and western areas, and the characteristics of hydrocarbon migration under the background of double-provenance were sorted out. The study results show that the crude oil in the eastern area has different Pr/Ph and sterane distribution from that in the western area. The oil and gas primarily migrated vertically. The high-quality source rocks and favorable source-reservoir-cap combinations lay the foundation for large-scale oil and gas accumulations. Vertically, the oil and gas enrichment is controlled by the scale of sandbody and the difference of physical properties, while on the plane, it is controlled by the connectivity of sandbodies, the argillaceous laminae between source rock and reservoir, the reservoir physical property and the fractures. The sandbodies of oil-rich zones in the eastern and western areas have large thickness, low shale content, good physical properties, weak heterogeneity, few argillaceous laminae and abundant fractures, all of which are favorable for the vertical migration and accumulation of oil and gas. In contrast, in the middle area with converging provenances, the reservoirs, composed of thin sandbodies, features rapid variation in lithology and physical properties, strong heterogeneity, poor continuity of sandbodies, abundant argillaceous laminae between source rock and reservoir, and few fractures, makes it difficult for the oil and gas to migrate vertically, and results in low oil enrichment degree ultimately. For the exploration of continental multiple-provenance tight reservoirs, not only the good-property source rocks and reservoirs, but more importantly the source-reservoir contact relationship and the effect of fractures on the hydrocarbon migration and accumulation should be considered. | CHEN Shijia LEI Junjie LIU Chun YAO Jingli LI Yong LI Shixiang SU Kaiming XIAO Zhenglu | 2019 | Petroleum Exploration and Development2019，46，2： | 0 |
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