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| 1 | Genetic and biological characteristics of the globally circulating H5N8 avian influenza viruses and the protective efficacy offered by the poultry vaccine currently used in China显示文摘The H5N8 avian influenza viruses have been widely circulating in wild birds and are responsible for the loss of over 33 million domestic poultry in Europe, Russia, Middle East, and Asia since January 2020. To monitor the invasion and spread of the H5N8 virus in China, we performed active surveillance by analyzing 317 wild bird samples and swab samples collected from 41,172 poultry all over the country. We isolated 22 H5N8 viruses from wild birds and 14 H5N8 viruses from waterfowls. Genetic analysis indicated that the 36 viruses formed two different genotypes: one genotype viruses were widely detected from different wild birds and domestic waterfowls;the other genotype was isolated from a whopper swan. We further revealed the origin and spatiotemporal spread of these two distinct H5N8 virus genotypes in 2020 and 2021. Animal studies indicated that the H5N8 isolates are highly pathogenic to chickens, mildly pathogenic in ducks, but have distinct pathotypes in mice. Moreover, we found that vaccinated poultry in China could be completely protected against H5N8 virus challenge. Given that the H5N8 viruses are likely to continue to spread in wild birds, vaccination of poultry is highly recommended in high-risk countries to prevent H5N8 avian influenza. | Pengfei Cui Xianying Zeng Xuyong Li Yanbing Li Jianzhong Shi Conghui Zhao Zhiyuan Qu Yanwen Wang Jing Guo Wenli Gu Qi Ma Yuancheng Zhang Weipeng Lin Minghui Li Jingman Tian Dongxue Wang Xin Xing Yanjing Liu Shuxin Pan Yaping Zhang Hongmei Bao Liling Liu Guobin Tian Chengjun Li Guohua Deng Hualan Chen | 2022 | Science China(Life Sciences)2022，65，4： | 12 |
| 2 | Development trend and prospect of solid phase extraction technology显示文摘Solid phase extraction is widely used in sample pretreatment,concentration and analysis processes due to high selectivity and suitability for low concentration sample system.In this review,we systematically summarized and discussed the development trends of solid phase extraction by bibliometrics method.By analyzing papers output scale,the research and development direction of solid phase extraction technology is prospected.We also give an overview on current strategies of novel solid phase extraction from the separation medium and separation technology.The paper aims to describe the global research profile and the development trends of solid phase extraction,to help researchers to accurately grasp the research trend and to provide support for scientific research institutions to formulate scientific policies and strategic plans.Furthermore,the prospect of the development and application of solid phase extraction is also discussed. | Chao Zhang Huifang Xing Liangrong Yang Pengfei Fei Huizhou Liu | 2022 | Chinese Journal of Chemical Engineering2022，35，2： | 9 |
| 3 | On the modification of hypereutectic Al-Si alloys using rare earth Er显示文摘Hypereutectic Al-17.5Si (wt pct) and Al-25Si (wt pct) alloys with various content of rare earth Er were prepared by conventional casting technique. The effect of Er on the microstructure and properties of the hypereutectic Al-Si alloys was investigated using optical microscopy, scanning electron microscopy as well as friction and wear tests. It was found that optimal amount of Er exists in modifying the hypereutectic Al-Si alloys. An appropriate addition of Er improved the anti-wear properties, and reduced the friction coefficient of the hypereutectic Al-Si alloys studied. The primary silicon crystals in the hypereutectic Al-Si alloys were refined with the suitable addition of Er element. The modification mechanism was also discussed in this paper. | Pengfei XING Bo GAO Yanxin ZHUANG Kaihua LIU Ganfeng TU | 2010 | Acta Metallurgica Sinica(English Letters)2010，23，5： | 7 |
| 4 | New innovations in pavement materials and engineering:A review on pavement engineering research 2021显示文摘Sustainable and resilient pavement infrastructure is critical for current economic and environmental challenges.In the past 10 years,the pavement infrastructure strongly supports the rapid development of the global social economy.New theories,new methods,new technologies and new materials related to pavement engineering are emerging.Deterioration of pavement infrastructure is a typical multi-physics problem.Because of actual coupled behaviors of traffic and environmental conditions,predictions of pavement service life become more and more complicated and require a deep knowledge of pavement material analysis.In order to summarize the current and determine the future research of pavement engineering,Journal of Traffic and Transportation Engineering(English Edition)has launched a review paper on the topic of'New innovations in pavement materials and engineering:A review on pavement engineering research 2021'.Based on the joint-effort of 43 scholars from 24 well-known universities in highway engineering,this review paper systematically analyzes the research status and future development direction of 5 major fields of pavement engineering in the world.The content includes asphalt binder performance and modeling,mixture performance and modeling of pavement materials,multi-scale mechanics,green and sustainable pavement,and intelligent pavement.Overall,this review paper is able to provide references and insights for researchers and engineers in the field of pavement engineering. | JTTE Editorial Office Jiaqi Chen Hancheng Dan Yongjie Ding Yangming Gao Meng Guo Shuaicheng Guo Bingye Han Bin Hong Yue Hou Chichun Hu Jing Hu Ju Huyan Jiwang Jiang Wei Jiang Cheng Li Pengfei Liu Yu Liu Zhuangzhuang Liu Guoyang Lu Jian Ouyang Xin Qu Dongya Ren Chao Wang Chaohui Wang Dawei Wang Di Wang Hainian Wang Haopeng Wang Yue Xiao Chao Xing Huining Xu Yu Yan Xu Yang Lingyun You Zhanping You Bin Yu Huayang Yu Huanan Yu Henglong Zhang Jizhe Zhang Changhong Zhou Changjun Zhou Xingyi Zhu | 2021 | Journal of Traffic and Transportation Engineering(English Edition)2021，8，6： | 7 |
| 5 | A direct H2O2 production based on hollow porous carbon sphere-sulfur nanocrystal composites by confinement effect as oxygen reduction electrocatalysts显示文摘Carbon-sulfur composites have draw n in creasing interest in various fields including electrocatalysis because of their unique structures.However,carb on-sulfur composite with tiny sulfur nano crystals has still received little attention.Herein,hollow porous carb on sphere-sulfur composite(HPCS-S)which possesses excellent electrochemical performance towards H2O2 has been prepared for the first time via a simple silica template method.The 2-5 nm sulfur nan ocrystals being restricted in the cha nnel of the hollow porous carb on spheres are un der a strong compressive stress,which was further con firmed by high-resoluti on tran smissi on electr on microscopy(HRTEM)and GPA.The HPCS-S nano crystals show better con ductivity tha n amorphous sulfur clusters because of the reducti on of the steric hindrance which efficie ntly promotes the electron transportation.Consequently,the higher activity and selectivity towards the 2e^oxygen reduction reaction(ORR)to H2O2 in alkaline solution was obtained.The H2O2 selectivity rises from 20%to over 70%after the sulfur addition and the H2O2 production rate achieves 183.99 mmol-gcataiyst-1 with the Faradaic efficiency of 70%.Furthermore,performance enhancement mechanism was also investigated using the den sity functional theory(DFT)calculatio ns.After the in troduci ng of sulfur nano crystals,the appeara nee of S-S bond greatly decreases the overpotential compared with S-doping,which results in significant enhancement of the electrocatalytic property.Consequently,the HPCS-S can be an efficient H2O2 production electrocatalyst in alkaline solution. | Guanyu Chen Jiwei Liu Qingqing Li Pengfei Guan Xuefeng Yu Linshen Xing Jie Zhang Renchao Che | 2019 | Nano Research2019，12，10： | 6 |
| 6 | Superior performance and high service stability for Ge Te-based thermoelectric compounds显示文摘GeTe-based compounds have been intensively studied recently due to their superior thermoelectric performance,but their real applications are still limited so far due to the drastic volume variation that occurs during the rhombohedral–cubic phase transition,which may break the material or the material/electrode interface during service.Here,superior performance and high service stability for GeTe-based thermoelectric compounds are achieved by co-doping Mg and Sb into GeTe.The linear coefficient of thermal expansion before phase transition is greatly improved to match that after phase transition,yielding smooth volume variation around the phase transition temperature.Likewise,co-doping(Mg,Sb)in GeTe successfully tunes the carrier concentration to the optimal range and effectively suppresses the lattice thermal conductivity.A peak z T of 1.84 at 800 K and an average z T of 1.2 in 300–800 K have been achieved in Ge0.85Mg0.05Sb0.1Te.Finally,a Ni/Ti/Ge0.85Mg0.05Sb0.1Te thermoelectric uni-leg is fabricated and tested,showing quite good service stability even after 450 thermal cycles between 473 K and 800 K.This study will accelerate the application of GeTe-based compounds for power generation in the mid-temperature range. | Tong Xing Qingfeng Song Pengfei Qiu Qihao Zhang Xugui Xia Jincheng Liao Ruiheng Liu Hui Huang Jiong Yang Shengqiang Bai Dudi Ren Xun Shi Lidong Chen | 2019 | National Science Review2019，6，5： | 4 |
| 7 | Ammonia leaching mechanism and kinetics of LiCoO_(2) material from spent lithium-ion batteries显示文摘In this paper,the ammonia leaching process and high-energy ball milling method were adapted to recover spent LiCoO_(2) material.The ammonia reduction leaching mechanism of LiCoO_(2) material in the ammonia-sodium sulfite-ammonium chloride system was elucidated.Compared with untreated LiCoO_(2) material,the leaching equilibrium time of LiCoO_(2) after ball-milled for 5 h was reduced from 48 h to 4 h,and the leaching efficiency of lithium and cobalt was improved from 69.86%and 70.80%to 89.86%and98.22%,respectively.Importantly,the apparent activation energy and leaching kinetic equation of the reaction was calculated by the shrinking core reaction model,indicating that the reaction was controlled by the chemical reaction. | Dongmin Li Bao Zhang Xing Ou Jiafeng Zhang Kui Meng Guanjun Ji Pengfei Li Jianhui Xu | 2021 | Chinese Chemical Letters2021，32，7： | 2 |
| 8 | Active and stable Cu doped NiMgAlO catalysts for upgrading ethanol to n-butanol显示文摘Upgrading ethanol to n-butanol is an attractive way for renewable n-butanol production. Herein, Cu was selected to modify NiMgAlO catalysts for improving ethanol conversion and n-butanol selectivity. Over the optimized 2%Cu-NiMgAlO catalyst, ethanol conversion and n-butanol selectivity were enhanced to 30.0% and 64.2%, respectively, in 200 h time on stream at 523 K. According to physicochemical characterizations and theoretical calculations, the key role of multiple active sites in this reaction was extensively investigated. The plate-like structure of hydrotalcite was maintained over 2%Cu-NiMgAlO catalysts, with an average Ni particle size of ca. 5.4 nm. The presence of Cu species created CuNi alloy sites and Lewis acid-base pairs, and increased hydrogen transfer and condensation reactions, resulting in elevated ethanol conversion and n-butanol selectivity. Additionally, CuNi alloy had a strong interaction with CuNiMgAl oxides, forming homogeneous boundary due to their close ionic radius and lattice matching, and afforded the long time stability in the ethanol to n-butanol reaction. | Zhinuo Wang Ming Yin Jifeng Pang Xianquan Li Yanan Xing Yang Su Shimin Liu Xiaoyan Liu Pengfei Wu Mingyuan Zheng Tao Zhang | 2022 | Journal of Energy Chemistry2022，31，9： | 2 |
| 9 | Auxin Binding Protein 1 Reinforces Resistance to Sugarcane Mosaic Virus in Maize显示文摘 | Pengfei Leng Qing Ji Torben Asp Ursula K. Frei Christina R. Ingvardsen Yongzhong Xing Bruno Studer Margaret Redinbaugh Mark Jones Priyanka Gajjar Sisi Liu Fei Li Guangtang Pan Mingliang Xu Thomas Lǖbberstedt | 2017 | Molecular Plant2017，10，10： | 2 |
| 10 | Catch bond-inspired hydrogels with repeatable and loading rate-sensitive specific adhesion显示文摘Biological receptor-ligand adhesion governed by mammalian cells involves a series of mechanochemical pro-cesses that can realize reversible,loading rate-dependent specific interfacial bonding,and even exhibit a counterintuitive behavior called catch bonds that tend to have much longer lifetimes when larger pulling forces are applied.Inspired by these catch bonds,we designed a hydrogen bonding-meditated hydrogel made from acrylic acid-N-acryloyl glycinamide(AA-NAGA)copolymers and tannic acids(TA),which formed repeatable specific adhesion to polar surfaces in an ultra-fast and robust way,but hardly adhered to nonpolar materials.It demonstrated up to five-fold increase in shear adhesive strength and interfacial adhesive toughness with external loading rates varying from 5 to 500 mm min^(-1).With a mechanochemical coupling model based on Monte Carlo simulations,we quantitatively revealed the nonlinear dependence of rate-sensitive interfacial adhesion on external loading,which was in good agreement with the experimental data.Likewise,the developed hydrogels were biocompatible,possessed antioxidant and antibacterial properties and promoted wound healing.This work not only reports a stimuli-responsive hydrogel adhesive suitable for multiple biomedical applications,but also offers an innovative strategy for bionic designs of smart hydrogels with loading rate-sensitive specific adhesion for various emerging areas including flexible electronics and soft robotics. | Zuoying Yuan Xiaocen Duan Xing Su Zhuoling Tian Anqi Jiang Zhuo Wan Hao Wang Pengfei Wei Bo Zhao Xiaozhi Liu Jianyong Huang | 2023 | Bioactive Materials2023，，3： | 1 |
| 11 | Antitumor synergism between PAK4 silencing and immunogenic phototherapy of engineered extracellular vesicles显示文摘Immunotherapy has revolutionized the landscape of cancer treatment.However,single immunotherapy only works well in a small subset of patients.Combined immunotherapy with antitumor synergism holds considerable potential to boost the therapeutic outcome.Nevertheless,the synergistic,additive or antagonistic antitumor effects of combined immunotherapies have been rarely explored.Herein,we established a novel combined cancer treatment modality by synergizing p21-activated kinase 4(PAK4)silencing with immunogenic phototherapy in engineered extracellular vesicles(EVs)that were fabricated by coating M1 macrophage-derived EVs on the surface of the nano-complex cores assembled with si RNA against PAK4 and a photoactivatable polyethyleneimine.The engineered EVs induced potent PAK4 silencing and robust immunogenic phototherapy,thus contributing to effective antitumor effects in vitro and in vivo.Moreover,the antitumor synergism of the combined treatment was quantitatively determined by the Compu Syn method.The combination index(CI)and isobologram results confirmed that there was an antitumor synergism for the combined treatment.Furthermore,the dose reduction index(DRI)showed favorable dose reduction,revealing lower toxicity and higher biocompatibility of the engineered EVs.Collectively,the study presents a synergistically potentiated cancer treatment modality by combining PAK4 silencing with immunogenic phototherapy in engineered EVs,which is promising for boosting the therapeutic outcome of cancer immunotherapy. | Mei Lu Haonan Xing Wanxuan Shao Pengfei Wu Yuchuan Fan Huining He Stefan Barth Aiping Zheng Xing-Jie Liang Yuanyu Huang | 2023 | Acta Pharmaceutica Sinica B2023，13，9： | 1 |
| 12 | Differential SW16.1 allelic effects and genetic backgrounds contributed to increased seed weight after soybean domestication显示文摘Although seed weight has increased following domestication from wild soybean(Glycine soja) to cultivated soybean(Glycine max), the genetic basis underlying this change is unclear. Using mapping populations derived from chromosome segment substitution lines of wild soybean, we identified SW16.1 as the causative gene underlying a major quantitative trait locus controlling seed weight.SW16.1 encodes a nucleus-localized LIM domaincontaining protein. Importantly, the GsSW16.1 allele from wild soybean accession N24852 had a negative effect on seed weight, whereas the GmSW16.1 allele from cultivar NN1138-2 had a positive effect. Gene expression network analysis,reverse-transcription quantitative polymerase chain reaction, and promoter-luciferase reporter transient expression assays suggested that SW16.1 regulates the transcription of MT4, a positive regulator of seed weight. The natural variations in SW16.1 and other known seed weight genes were analyzed in soybean germplasm. The SW16.1 polymorphism was associated with seed weight in 247 soybean accessions, showing much higher frequency of positive-effect alleles in cultivated soybean than in wild soybean. Interestingly,gene allele matrix analysis of the known seed weight genes revealed that G. max has lost 38.5%of the G. soja alleles and that most of the lost alleles had negative effects on seed weight. Our results suggest that eliminating negative alleles from G. soja led to a higher frequency of positive alleles and changed genetic backgrounds in G. max,which contributed to larger seeds in cultivated soybean after domestication from wild soybean.Our findings provide new insights regarding soybean domestication and should assist current soybean breeding programs. | Xianlian Chen Cheng Liu Pengfei Guo Xiaoshuai Hao Yongpeng Pan Kai Zhang Wusheng Liu Lizhi Zhao Wei Luo Jianbo He Yanzhu Su Ting Jin Fenfen Jiang Si Wang Fangdong Liu Rongzhou Xie Changgen Zhen Wei Han Guangnan Xing Wubin Wang Shancen Zhao Yan Li Junyi Gai | 2023 | Journal of Integrative Plant Biology2023，65，7： | 1 |
| 13 | Effect of Co element on microstructure and mechanical properties of FeCo_x NiCuAl alloys显示文摘FeCox NiCuAl (x values in molar ratio, x=0.2, 0.5, 1, 1.5, 2 and 3) alloys were prepared using a suck-casting method. The effect of Co element on phase constituents, microstructure and mechanical properties of the FeCox NiCuAl alloys was investigated using X-ray diffraction, scanning electron microscopy, optical microscopy and compressive tests. It was found that the Co addition has a significant influence on the structure and properties of the FeCox NiCuAl alloys. The alloys have typical dendrite microstructure, and are composed of a simple fcc structure and bcc structure. The addition of Co promotes the formation of fcc phase in the alloys, retards the compressive strength and hardness of the alloys, and enhances the plasticity of the alloys. | Yanxin ZHUANG Wenjie LIU Pengfei XING Fei WANG Jicheng HE | 2012 | Acta Metallurgica Sinica(English Letters)2012，25，2： | 1 |
| 14 | Innovative preparation of Co@Cu Fe_(2)O_(4) composite via ball-milling assisted chemical precipitation and annealing for glorious electromagnetic wave absorption显示文摘To deal with the growing electromagnetic hazards,herein a Co@CuFe_(2)O_(4)absorbing agent with excellent impedance matching at thin thickness was obtained via an innovative route of ball-milling assisted chemical precipitation and annealing.The as-prepared composite possesses excellent interface polarization ability due to sufficient contact between CuFe_(2)O_(4)NPs and flat Co,and this compressed Co lamella can also provide sufficient eddy current loss.Moreover,the dipole polarization,electron hopping/conduction,and structural scattering also contribute to the broadband microwave absorption of the composite.Thus,the minimum microwave reflection loss achieves-35.56 d B at12.93 GHz for 1.8 mm thickness,and the broadest efficient absorption bandwidth can reach 6.74 GHz for a thinner thickness of 1.72 mm.The preparation method reported here can be referenced as a new-type route to manufacture electromagnetic absorbers with outstanding performance. | Xing Feng Pengfei Yin Limin Zhang Xiyuan Sun Jian Wang Liang Zhao Changfang Lu Zhihua Gao Yongxin Zhan | 2023 | International Journal of Minerals,Metallurgy and Materials2023，30，3： | 1 |
| 15 | The First High-quality Reference Genome of Sika Deer Provides Insights into High-tannin Adaptation显示文摘Sika deer are known to prefer oak leaves,which are rich in tannins and toxic to most mammals;however,the genetic mechanisms underlying their unique ability to adapt to living in the jungle are still unclear.In identifying the mechanism responsible for the tolerance of a highly toxic diet,we have made a major advancement by explaining the genome of sika deer.We generated the first high-quality,chromosome-level genome assembly of sika deer and measured the correlation between tannin intake and RNA expression in 15 tissues through 180 experiments.Comparative genome analyses showed that the UGT and CYP gene families are functionally involved in the adaptation of sika deer to high-tannin food,especially the expansion of the UGT family 2 subfamily B of UGT genes.The first chromosome-level assembly and genetic characterization of the tolerance to a highly toxic diet suggest that the sika deer genome may serve as an essential resource for understanding evolutionary events and tannin adaptation.Our study provides a paradigm of comparative expressive genomics that can be applied to the study of unique biological features in non-model animals. | Xiumei Xing Cheng Ai Tianjiao Wang Yang Li Huitao Liu Pengfei Hu Guiwu Wang Huamiao Liu Hongliang Wang Ranran Zhang Junjun Zheng Xiaobo Wang Lei Wang Yuxiao Chang Qian Qian Jinghua Yu Lixin Tang Shigang Wu Xiujuan Shao Alun Li Peng Cui Wei Zhan Sheng Zhao Zhichao Wu Xiqun Shao Yimeng Dong Min Rong Yihong Tan Xuezhe Cui Shuzhuo Chang Xingchao Song Tongao Yang Limin Sun Yan Ju Pei Zhao Huanhuan Fan Ying Liu Xinhui Wang Wanyun Yang Min Yang Tao Wei Shanshan Song Jiaping Xu Zhigang Yue Qiqi Liang Chunyi Li Jue Ruan Fuhe Yang | 2023 | Genomics, Proteomics & Bioinformatics2023，21，1： | 1 |
| 16 | Preparation of Bi2WO6 and Its Ultra-Deep Oxidative Desulfurization Performance in Ionic Liquids显示文摘The micro-spheric Bi_2WO_6 was synthesized by a simple one-step hydrothermal method. Bi_2WO_6 crystals were characterized using XRD, SEM, EDS and BET techniques. Bi_2WO_6, H_2O_2 and [HMIM][BF_4] served as catalyst, oxidant and extracting agent in the oxidative desulfurization system(ODS), respectively. The influence of extraction agent type, oxidant usage, catalyst dosage, temperature, sulfur compound type and other factors on the oxidative desulfurization was studied in the present work. The experimental results demonstrate that Bi_2WO_6 possesses high activity for desulfurization of dibenzothiophene(DBT) and benzothiophene(BT). The desulfurization rate of DBT and BT in model oil could reach 98.1% in 80 minutes and 96.2% in 120min at 70℃, respectively. Moreover, the desulfurization performance of catalyst for DBT hardly changed after being recycled for 10 times. | Xing Pengfei Zhao Rongxiang Li Xiuping Gao Xiaohan | 2017 | China Petroleum Processing & Petrochemical Technology2017，19，1： | 1 |
| 17 | Formability enhancement in hot spinning of titanium alloy thin-walled tube via prediction and control of ductile fracture显示文摘The damage and fracture in hot spinning of titanium alloy is a very complex process under the combined effects of microstructure evolution and stress state.In this study,their dependences on processing parameters were investigated by an integrated FE model considering microstructure and damage evolution,and revealing the effects of microstructure and stress states on damage evolution.The results show that the inner surface of workpiece with the largest voids volume fraction is the place with the greatest potential of fracture.This is mainly attributed to the superposition effects of positive stress triaxiality and the smallest dynamic recrystallization(DRX)fraction andβphase fraction at the inner surface.The damage degree is decreased gradually with the increase of initial spinning temperature and roller fillet radius.Meanwhile,it is first decreased and then increased with the increases of spinning pass and roller feed rate,which can be explained based on the variations ofβphase fraction,DRX fraction,stress state and tensile plastic strain with processing parameters.In addition,the dominant influencing mechanisms were identified and discussed.Finally,the thickness reduction without defect in the hot spinning of TA15 alloy tube is greatly increased by proposing an optimal processing scheme. | Pengfei GAO Chao YU Mingwang FU Lu XING Mei ZHAN Jing GUO | 2022 | Chinese Journal of Aeronautics2022，35，2： | 1 |
| 18 | Observation of non-Markovianity at room temperature by prolonging entanglement in solids显示文摘Open quantum systems are always exposed to an external environment,which result in interacting and exchanging information between quantum systems and their surroundings.The dynamics of real open quantum systems are often expected to deviate from the idealized Markovian process of losing information to their surrounding environment and to exhibit non-Markovian | Shijie Peng Xiangkun Xu Kebiao Xu Pu Huang Pengfei Wang Xi Kong Xing Rong Fazhan Shi Changkui Duan Jiangfeng Du | 2018 | Science Bulletin2018，63，6： | 1 |
| 19 | Fixation of hybrid sterility genes and favorable alleles of key yield-related genes with dominance contribute to the high yield of the Yongyou series of intersubspecific hybrid rice显示文摘In rice,the Yongyou series of Xian-Geng intersubspecific hybrids have excellent production performance,as shown by their extremely high yield.However,the mechanisms underlying the success of these rice hybrids are unclear.In this study,three F2 populations are generated from three Yongyou hybrids to determine the genetic basis of the extremely high yield of intersubspecific hybrids.Genome constitution analysis reveals that the female and male parental lines belong to the Geng and Xian subspecies,respectively,although introgression of 20%of the Xian ancestry and 14%of the Geng ancestry are observed.Twenty-five percent of the hybrid genomes carries homozygous Xian or Geng fragments,which harbors hybrid sterility genes such as Sd,Sc,f5,and q S12 and favorable alleles of key yield-related genes,including NAL1,Ghd7,and Ghd8.None of the parents carries the S5+killer of the S5 killer-protector system.Compatible allele combinations of hybrid sterility genes ensure the fertility of these intersubspecific hybrids and overcome the bottleneck in applying intersubspecific hybrids.Additive effects of favorable alleles of yield-related genes fixed in both parents enhances midparent values.Many QTLs for yield and its key component spikelets per panicle shows dominance and the net positive dominant effects lead to heterosis.These factors result in an extremely high yield of the hybrids.These findings will aid in the development of new intersubspecific rice hybrids with diverse genetic backgrounds. | Pengfei Wang Feixiang Qi Honglin Yao Xingbing Xu Wenjun Li Jianghu Meng Qinglu Zhang Weibo Xie Yongzhong Xing | 2022 | Journal of Genetics and Genomics2022，49，5： | 1 |
| 20 | Tild-CRISPR可在小鼠和人类细胞中实现高效、精确的基因敲入显示文摘文章简介研究团队基于CRISPR/Cas9系统,设计了一种新的靶向整合策略Tild-CRISPR,通过PCR扩增或者精确酶切,获得含有800bp同源臂的转基因供体与Cas9 mRNA以及single-guide RNA一起注射到小鼠受精卵中。 | 姚璇 Meiling Zhang Xing Wang Wenqin Ying Xinde Hu Pengfei Dai Feilong Meng Linyu Shi Yun Sun Ning Yao Wanxia Zhong Yun Li Keliang Wu 李卫平 陈子江 杨辉 | 2019 | 科学新闻2019，0，2： | 0 |
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