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| 1 | Autophagy inhibition enhances apigenin-induced apoptosis in human breast cancer cells显示文摘Apigenin (4',5,7-trihydroxyflavone) is a member of the flavone subclass of flavonoids present in fruits and vegetables. The involvement of autophagy in the apigenin-induced apoptotic death of human breast cancer cells was investigated. Cell proliferation and viability were assessed by 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) and clonogenic assays. Flow cytometry, fluorescent staining and Western blot analysis were employed to detect apoptosis and autophagy, and the role of autophagy was assessed using autophagy inhibitors. Apigenin dose- and time-dependently repressed the proliferation and clonogenic survival of the human breast cancer T47D and MDA-MB-231 cell lines. The death of T47D and MDA-MB-231 cells was due to apoptosis associated with increased levels of Caspase3, PARP cleavage and Bax/Bcl-2 ratios. The results from flow cytometry and fluorescent staining also verified the occurrence of apoptosis. In addition, the apigenin-treated cells exhibited autophagy, as characterized by the appearance of autophagosomes under fluorescence microscopy and the accumulation of acidic vesicular organelles (AVOs) by flow cytometry. Furthermore, the results of the Western blot analysis revealed that the level of LC3-II, the processed form of LC3-I, was increased. Treatment with the autophagy inhibitor, 3-methyladenine (3-MA), significantly enhanced the apoptosis induced by apigenin, which was accompanied by an increase in the level of PARP cleavage. Similar results were also confirmed by flow cytometry and fluorescence microscopy. These results indicate that apigenin has apoptosis-and autophagy-inducing effects in breast cancer cells. Autophagy plays a cyto-protective role in apigenin-induced apoptosis, and the combination of apigenin and an autophagy inhibitor may be a promising strategy for breast cancer control. | Xuchen Cao Bowen Liu Wenfeng Cao Weiran Zhang Fei Zhang Hongmeng Zhao Ran Meng Lin Zhang Ruifang Niu Xishan Hao Bin Zhang | 2013 | Chinese Journal of Cancer Research2013，25，2： | 12 |
| 2 | The Ghd7 transcription factor represses ARE1 expression to enhance nitrogen utilization and grain yield in rice显示文摘The genetic improvement of nitrogen use efficiency(NUE)of crops is vital for grain productivity and sustainable agriculture.However,the regulatory mechanism of NUE remains largely elusive.Here,we report that the rice Grain number,plant height,and heading date7(Ghd7)gene genetically acts upstream of ABC1 REPRESSOR1(ARE1),a negative regulator of NUE,to positively regulate nitrogen utilization.As a transcriptional repressor,Ghd7 directly binds to two Evening Element-like motifs in the promoter and intron 1 of ARE1,likely in a cooperative manner,to repress its expression.Ghd7 and ARE1 display diurnal expression patterns in an inverse oscillation manner,mirroring a regulatory scheme based on these two loci.Analysis of a panel of 2656 rice varieties suggests that the elite alleles of Ghd7 and ARE1 have undergone diversifying selection during breeding.Moreover,the allelic distribution of Ghd7 and ARE1 is associated with the soil nitrogen deposition rate in East Asia and South Asia.Remarkably,the combination of the Ghd7 and ARE1 elite alleles substantially improves NUE and yield performance under nitrogen-limiting conditions.Collectively,these results define a Ghd7–ARE1-based regulatory mechanism of nitrogen utilization,providing useful targets for genetic improvement of rice NUE. | Qing Wang Qingmei Su Jinqiang Nian Jian Zhang Meng Guo Guojun Dong Jiang Hu Rongsheng Wang Changshuo Wei Guanwen Li Wan Wang Hui-Shan Guo Shaoyang Lin Wenfeng Qian XianzhiXie Qian Qian Fan Chen Jianru Zuo | 2021 | Molecular Plant2021，14，6： | 10 |
| 3 | A Methodology for Reliability of WSN Based on Software Defined Network in Adaptive Industrial Environment显示文摘As communication technology and smart manufacturing have developed, the industrial internet of things(IIo T)has gained considerable attention from academia and industry.Wireless sensor networks(WSNs) have many advantages with broad applications in many areas including environmental monitoring, which makes it a very important part of IIo T. However,energy depletion and hardware malfunctions can lead to node failures in WSNs. The industrial environment can also impact the wireless channel transmission, leading to network reliability problems, even with tightly coupled control and data planes in traditional networks, which obviously also enhances network management cost and complexity. In this paper, we introduce a new software defined network(SDN), and modify this network to propose a framework called the improved software defined wireless sensor network(improved SD-WSN). This proposed framework can address the following issues. 1) For a large scale heterogeneous network, it solves the problem of network management and smooth merging of a WSN into IIo T. 2) The network coverage problem is solved which improves the network reliability. 3) The framework addresses node failure due to various problems, particularly related to energy consumption.Therefore, it is necessary to improve the reliability of wireless sensor networks, by developing certain schemes to reduce energy consumption and the delay time of network nodes under IIo T conditions. Experiments have shown that the improved approach significantly reduces the energy consumption of nodes and the delay time, thus improving the reliability of WSN. | Ying Duan Wenfeng Li Xiuwen Fu Yun Luo Lin Yang | 2018 | IEEE/CAA Journal of Automatica Sinica2018，5，1： | 7 |
| 4 | Variation of spatio-temporal distribution of on-road vehicle emissions based on real-time RFID data显示文摘High-resolution vehicular emissions inventories are important for managing vehicular pollution and improving urban air quality. This study developed a vehicular emission inventory with high spatio-temporal resolution in the main urban area of Chongqing, based on realtime traffic data from 820 RFID detectors covering 454 roads, and the differences in spatiotemporal emission characteristics between inner and outer districts were analysed. The result showed that the daily vehicular emission intensities of CO, hydrocarbons, PM2.5, PM10,and NO_(x) were 30.24, 3.83, 0.18, 0.20, and 8.65 kg/km per day, respectively, in the study area during 2018. The pollutants emission intensities in inner district were higher than those in outer district. Light passenger cars(LPCs) were the main contributors of all-day CO emissions in the inner and outer districts, from which the contributors of NO_(x) emissions were different. Diesel and natural gas buses were major contributors of daytime NO_(x) emissions in inner districts, accounting for 40.40%, but buses and heavy duty trucks(HDTs) were major contributors in outer districts. At nighttime, due to the lifting of truck restrictions and suspension of buses, HDTs become the main NO_(x) contributor in both inner and outer districts,and its three NO_(x) emission peak hours were found, which are different to the peak hours of total NO_(x) emission by all vehicles. Unlike most other cities, bridges and connecting channels are always emission hotspots due to long-time traffic congestion. This knowledge will help fully understand vehicular emissions characteristics and is useful for policymakers to design precise prevention and control measures. | Yonghong Liu Wenfeng Huang Xiaofang Lin Rui Xu Li Li Hui Ding | 2022 | Journal of Environmental Sciences2022，34，6： | 2 |
| 5 | A highly efficient and porous catalyst for simultaneous removal of NOx and diesel soot显示文摘 | Xiaosheng Peng He Lin Wenfeng Shangguan | 2007 | Catalysis Communications2007，2，8： | 1 |
| 6 | A highly efficient and porous catalyst for simultaneous removal of NOx and diesel soot显示文摘 | Peng Xiaosheng Lin He Shangguan Wenfeng | 2007 | Catalysis Communica- tions2007，8，： | 1 |
| 7 | Electrochemical hydrogen storage properties of Mg100-xNix produced by hydriding combustion synthesis and mechanical milling显示文摘In this work, Mg-based hydrogen storage composites with an initial 100-x: x(x=25, 32.3, 50, 66.7) of Mg:Ni molar ratio were prepared by HCS+MM and their phase compositions and electrochemical performances were investigated in detail. The results show that the composites with desirable constituents can be achieved by adjusting the molar ratio of the starting materials in the HCS process. Particularly, the HCS product of Mg_(67.7)Ni_(32.3) consists of the principal phase Mg_2NiH_4 and minor phase Mg_2NiH_(0.3). The dominate phase varies from Mg_2NiH_(0.3) and MgH_2 for the Mg enriched sample(x < 32.3) to MgNi_2 and Ni for the Ni enriched sample(x > 32.3). The MM modification not only brings about grain refinement of the alloys, but also leads to phase transformation of part Mg_2NiH_4 to Mg_2NiH_(0.3) in the Mg_(67.7)Ni_(32.3)sample. Electrochemical tests indicate that each sample can reach its maximum discharge capacity at the first cycle. Mg_(67.7)Ni_(32.3) displays the highest discharge capacity as well as a superior electrochemical kinetics owing to its excellent H atom diffusion ability and lower charge-transfer resistance. The Mg_(67.7)Ni_(32.3) provides the most optimized Mg/Ni atomic ratio considering the comprehensive electrochemical properties of all samples. | Delong Zhu Jiguang Zhang Yunfeng Zhu Yao Zhang Yana Liu Huaijun Lin Wenfeng Zhang Liquan Li | 2017 | Progress in Natural Science:Materials International2017，27，1： | 1 |
| 8 | Novel electrode structure for DMFC operated with liquid methanol显示文摘 | Shao Zhigang Lin Wenfeng Zhu Fuyun | 2006 | Electrochemistry Communications2006，8，1： | 1 |
| 9 | A tubular direct methanol fuel cell with Ti mesh anode显示文摘 | Shao Zhigang Lin Wenfeng Zhu Fuyun | 2006 | Journal of Power Sources2006，160，2： | 1 |
| 10 | A parametric study on the thermal performance of cross-corrugated solar air collectors 显示文摘 | Lin Wenxian Gao Wenfeng Liu Tao | 2006 | Applied Thermal Engineering2006，26，10： | 1 |
| 11 | Species turnover drives grassland community to phylogenetic clustering over long-term grazing disturbance显示文摘放牧通过改变草地群落物种组成和生物多样性,进而影响草地群落结构,对草地生态系统服务和功能产生深远的影响。然而,有关系统发育多样性和系统发育群落结构对长期放牧干扰的响应和适应的研究仍然很少,尤其是对于分布在极端环境中的生态系统。我们在青藏高原高寒草地上开展了多放牧强度的试验,探讨放牧干扰对植物系统发育多样性和群落结构的影响。研究发现,放牧干扰增加了植物群落的物种丰富度,促进了群落物种周转,从而改变了群落物种组成。低强度放牧对系统发育多样性和群落结构没有显著影响,而高强度放牧促使群落结构由分散向聚集变化。高强度放牧通过强烈的环境过滤作用,选择了一些耐牧的草地植物物种。在高强度放牧条件下,草地群落的聚集结构由近缘种的入侵和远缘种的丢失共同驱动。在植物功能性状水平上,我们发现与低强度放牧相比,高强度放牧通过改变根系深度对物种入侵产生影响,在很大程度上提升了物种的入侵性。我们的研究强调,仅仅利用物种丰富度和多样性并不能全面反映放牧干扰对草地群落的影响,而且在以后的放牧生态学研究中应该更加关注物种周转对群落系统发育多样性和群落结构的影响。 | Juntao Zhu Yangjian Zhang Wenfeng Wang Xian Yang Ning Chen Ruonan Shen Li Wang Lin Jiang | 2020 | Journal of Plant Ecology2020，13，2： | 1 |
| 12 | The muscle-specific microRNAs miR-1 and miR-133 produce opposing effects on apoptosis by targeting HSP60, HSP70 and caspase-9 in cardiomyocytes显示文摘 | Chaoqian Xu Yanjie Lu Zhenwei Pan Wenfeng Chu Xiaobin Luo Huixian Lin Jiening Xiao Hongli Shan Zhiguo Wang and Baofeng Yang | | Journal of Cell Science0，120，17： | 1 |
| 13 | An in-situ DRIFTS study in the reaction between NO_x and soot on BaAl_2O_4显示文摘The interactions between NO, O2 and their mixture on BaAl2O4, as well as the reaction of NOx with soot in the presence of O2, have been investigated using Diffuse Reflectance Infrared Fourier Transform Spectroscopy (DRIFTS). NO adsorption produces only nitrites species in the absence of O2. NO2 ad- sorption produces nitrates species besides nitrites species. The produced nitrites will further react with O2, O-su rf and O l2-att ice to form nitrates. The reaction of NOx with soot begins with the reaction of ni- trates with soot oxygenated complex (C(O)), which is regarded as the key and rate determining step. A reaction pathway is proposed for the catalyzed reaction of NOx with soot in the presence of O2. | LI YingJie LIN He SHANGGUAN WenFeng HUANG Zhen | 2008 | Chinese Science Bulletin2008，53，16： | 1 |
| 14 | A parametric study on the thermal performance of cross-corrugated solar air collectors 显示文摘 | LIN WENXIAN GAO WENFENG LIU TAO | 2006 | Applied Thermal Engineering2006，26，： | 1 |
| 15 | lncR-GAS5 upregulates the splicing factor SRSF10 to impair endothelial autophagy,leading to atherogenesis显示文摘Long noncoding RNAs(lncRNAs)play a critical role in the regulation of atherosclerosis.Here,we investigated the role of the lncRNA growth arrest-specific 5(lncR-GAS5)in atherogenesis.We found that the enforced expression of lncR-GAS5 contributed to the development of atherosclerosis,which presented as increased plaque size and reduced collagen content.Moreover,impaired autophagy was observed,as shown by a decreased LC3II/LC3I protein ratio and an elevated P62 level in lncR-GAS5-overexpressing human aortic endothelial cells.By contrast,lncR-GAS5 knockdown promoted autophagy.Moreover,serine/arginine-rich splicing factor 10(SRSF10)knockdown increased the LC3II/LC3I ratio and decreased the P62 level,thus enhancing the formation of autophagic vacuoles,autolysosomes,and autophagosomes.Mechanistically,lncR-GAS5 regulated the downstream splicing factor SRSF10 to impair autophagy in the endothelium,which was reversed by the knockdown of SRSF10.Further results revealed that overexpression of the lncR-GAS5-targeted gene miR-193-5p promoted autophagy and autophagic vacuole accumulation by repressing its direct target gene,SRSF10.Notably,miR-193-5p overexpression decreased plaque size and increased collagen content.Altogether,these findings demonstrate that lncR-GAS5 partially contributes to atherogenesis and plaque instability by impairing endothelial autophagy.In conclusion,lncR-GAS5 overexpression arrested endothelial autophagy through the miR-193-5p/SRSF10 signaling pathway.Thus,miR-193-5p/SRSF10 may serve as a novel treatment target for atherosclerosis. | Yuhua Fan Yue Zhang Hongrui Zhao Wenfeng Liu Wanqing Xu Lintong Jiang Ranchen Xu Yue Zheng Xueqing Tang Xiaohan Li Limin Zhao Xin Liu Yang Hong Yuan Lin Hui Chen Yong Zhang | 2023 | Frontiers of Medicine2023，17，2： | 1 |
| 16 | Copper hydroxide nano and microcrystal: Facile synthesis, shape evolution and their catalytic properties显示文摘 | Guanhua Lin Wenfeng Jia Wensheng Lu Long Jiang | 2010 | Journal of Colloid And Interface Science2010，，2： | 1 |
| 17 | A multiplex PCR assay for speciesidentification of raw and cooked bonito显示文摘 | LIN Wenfeng HWANG Dengwu | 2008 | Food Control2008，19，9： | 1 |
| 18 | A multiplex PCR assay for species identification of raw and cooked bonito显示文摘 | LIN Wenfeng HWANG Dengfwu | 2008 | Food Control2008，，19： | 1 |
| 19 | Clinical implications of ERβ methylation on sporadic breast cancers in Chinese women显示文摘 | Lin Zhao Zhaojin Yu Yunan Li Xiaoyan Wen Wenfeng Ma Lin Wang Jie Ren Caigang Liu Miao He Xuefeng Bai Mingli Sun Zhihong Zheng Xiaoyi Mi Enhua Wang Olufunmilayo I. Olopade Feng Jin Minjie Wei | 2012 | Medical Oncology2012，，3： | 1 |
| 20 | Approximate tool posture collision-free area generation for five-axis CNC finishing process using admissible area interpolation显示文摘 | Lin Zhiwei Shen Hongyao Gan Wenfeng Fu Jianzhong | 2012 | The International Journal of Advanced Manufacturing Technology2012，，9： | 1 |
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