|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | FADD and TRADD expression and apoptosis in primary hepatocellular carcinoma显示文摘AIM To investigate the clinical features ofFADD and TRADD expressions in primaryhepatocellular carcinoma(HCC)and todetermine their relationship with hepaticapoptosis.METHODS FADD and TRADD expressions weredetected by immunohistochemistry and hepaticapoptosis were determined by in situ end-labeling(ISEL).RESULTS Ten(25.6%)cases of HCC weredetected to express FADD protein.The positiverate in HCC is lower than that in non-cancerousadjacent liver tissues(62.5%)(P<0.05).Inthose of grade Ⅰ-Ⅱ,8(38.1%)cases wereFADD positive,while only 2/18(11.1%)casesof grade Ⅲ-Ⅳ had detectable FADD protein(P<0.05).No relationship was found betweenFADD expression and other clinical features,such as gender,age,tumor size,differentiationor metastasis.ISEL positive cells can be seen inall cases of HCC.The hepatic apoptosis wasassociated with FADD expression as moreapoptotic cells were detected in those caseswhich had moderately to strongly positiveFADD,as compared with negative or weakpositive FADD cases(P<0.05).No relationshipwas found between FADD expression and hepaticapoptosis in non-cancerous adjacent livertissues.Fifteen of 39(38.5%)cases of HCCwere found positive for TRADD protein,andsimilar positive rate(37.5%)in non-cancerousadjacent liver tissues(P>0.05).The expressionof TRADD is correlated with HCC differentiation, as only 22.2% of moderately to highlydifferentiated HCC showed positive TRADDprotein,while as high as 52.4% of poorlydifferentiated HCC had TRADD(P<0.05),Norelationship was found between TRADDexpression and gender,age,tumor size or gradeor metastasis,although 42.9% of HCC of gradeⅠ/Ⅱ showed positive TRADD which wasslightly higher than that of grade Ⅲ/Ⅳ(33.3%,P>0.05).Hepatic apoptosis was not related toTRADD expression in HCC or non-cancerousadjacent liver tissues.CONCLUSION Loss of FADD expression playsan important role in HCC carcinogenesis,andexpression of TRADD also contributes to HCCdevelopment.The cell apoptosis in HCC isassociated with FADD expression.However,theexpression of TRADD does not correlate wellwith hepatic apoptosis in HCC. | Sun BH Zhao XP Wang BJ Yang DL Hao LJ | 2000 | World Journal of Gastroenterology2000，6，2： | 22 |
| 2 | Cloning and expression of MXR7 gene in human HCC tissue显示文摘AIM To clone and identify the whole cDNA ofMXR7 gene and to find out its expression inhuman HCC,and normal tissues.METHODS The DNA primers were designed andsynthesized according to the whole cDNAsequence of MXR? gene.The cDNA of humanHCC was taken as the template while the cDNA ofMXR7 gene was synthesized by polymerasechain reaction(PCR).Recombinant DNAconforming to reading frame was constructed byconnecting purified PCR product of the cDNA ofMXR? gene with expression vector pGEX-5X-1 offusion protein.The plasmid MXRT/pGEX-5X-1was identified by sequencing.Using 32p labeledMXR? cDNA as probe,MXR7 mRNA expressionwas detected by Northern blot analysis in 12different human normal tissues,7 preoperativelyuntreated non-liver tumor tissues,30preoperatively untreated HCC,theparacancerous liver tissues and 12 normal livertissues samples.RESULTS Restriction enzyme and sequenceanalysis confirmed that the insertion sequence invector pGEX-5X-1 was the same as the cDNAsequence of MXR7 gene.Northern blot analysisshowed no expression of MXR? mRNA in 12 kindsof normal human tissues including liver,7 tumortissues in other sites and 12 normal livertissues,the frequencies of MXR7 mRNA expression in HCC and paracancerous livertissues were 76.6% and 13.3%,respectively.The frequency of MXR7 mRNA expression in HCCwithout elevation of serum AFP and in HCC<5cm was 90%(9/10)and 83.3%(5/6),respectively.CONCLUSION MXR7 mRNA is highly expressedin human HCC,which is specific and occurs at anearly stage of HCC,suggesting MXR7 mRNA canbe a tumor biomarker for HCC.The detection ofMXR7 mRNA expression in the biopsied livertissue is helpful in discovering early subclinicalliver cancer in those with negative serum AFP. | Zhou XP Wang HY Yang GS Chen ZJ Li BA Wu MC | 2000 | World Journal of Gastroenterology2000，6，1： | 23 |
| 3 | 门静脉高压并非肝细胞癌肝切除术的绝对禁忌证显示文摘在肝细胞癌(hepatocellular carcinoma,HCC)的各种临床分期系统中,Bruix J团队领衔的巴塞罗那肝癌临床分期标准(Barcelona Clinic Liver Cancer,BCLC)最受推崇,美国和欧洲肝病协会指南的制定亦基于该分期系统。BCLC认为门静脉高压显著增加HCC肝切除术后患者发生肝功能衰竭的风险, | Zhong JH Li H Xiao N Ye XP Ke Y Wang YY Ma L Chen J You XM Zhang ZY Lu SD Li LQ 钟鉴宏 齐鲁楠 | 2014 | 中国癌症防治杂志2014，6，4： | 18 |
| 4 | Magnetic resonance imaging of hepatocellular carcinoma induced by diethylnitrosamine in Sprague-Dawley rats显示文摘Diethylnitrosamine (DENA) is able to induce various benign and malignant liver lesions in rats with a high success rate and a low mortality rate. It provides a more appropriate model that better simulates the various lesions occurring in humans than the usual model of tumor implantations. The aim of the present study was to evaluate MRI liver examination in Sprague-Dawley(SD) rats as a routine method to detect hepatocellular carcinoma (HCC) nodules induced by DENA and to follow up their growth. METHODS:Hepatic carcinogenesis was induced in 80 male SD rats using oral DENA solution. All animals were imaged for liver tumor detection with a 1.5 Tesla magnet (Siemens Sonata,Erlangen, Germany) using correspondence scan parameters and a radio-frequency knee coil. Macroscopic examinations were performed along the axial MRI sections to evaluate magnetic resonance imaging (MRI) findings, and histopathological assessment was also performed. RESULTS:No false negative results were obtained on MR images. Hepatic tumors in 72 rats were confirmed macro-scopically and 68 rats were detected by MRI till the 20th week. The smallest and the largest nodules detected by MRI were 2 mm and 37 mm in diameter respectively. The agreement rate of MRI with macroscopic observation was 39. 1% and 97. 4% respectively for 2 mm to 5 mm and more than 5 mm nodules. CONCLUSIONS:The hepatic tumor induced by DENA provides a more representative range of tumors for imaging diagnosis and interventional treatment. MRI is the best approach for scrutinizing pathological changes of rat livers in the period of observation. | Department of Radiology, West China Hospital, Sichuan University, Chengdu 610041, China (Li X, Zhou XP and Guan YS) Department of Radiology, Ruijin Hospieal. Shanghai Second Medical University, Shanghai 200025 , China. Current address:Astra Zeneca R & D Alderley, Macclesfield SK 10 4 TG U. K. (Wang YX) | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，3： | 3 |
| 5 | Polymorphisms in DNA base excision repair genes ADPRT and XRCC1 and risk of lung cancer显示文摘 | Zhang XM Miao XP Liang G Hao BT Wang YG Tan W Li Y Guo YL He FC Wei QY Lin DX | 2006 | 中国生物学文摘2006，20，4： | 2 |
| 6 | Current study on epidemiology of pancreatic cancer 显示文摘 | WU JX WANG XP XU JY | 2000 | J Clin (in Chinese)2000，17，3： | 2 |
| 7 | Improved injectability and in vitro degradation of a calcium phosphate cement containing poly (lactide-co-glycolide) microspheres显示文摘 | Qi XP Ye JD Wang YJ | 2008 | Acta Biomater2008，4，6： | 1 |
| 8 | Antioxidant potential of ethanolic extract of Poiygonum cuspidatum and application in peanut oil显示文摘 | Pan YM Zhang XP Wang HS | 2007 | Food Chem2007，105，： | 1 |
| 9 | Enhancement of radiation cytotoxieity in breast-cancer cells by localized attachment of gold nanoparticles 显示文摘 | Kong T Zeng J Wang XP | 2008 | Small2008，4，9： | 1 |
| 10 | Toxic effects of over-load copper chloride on primary cultured rat asrocytes显示文摘 | Hu HL Wang XP Zhao YL | 2012 | Parkinsonism Relat Disord2012，18，： | 1 |
| 11 | Somatostatin and its receptors in the development of the endocrine pancreas显示文摘 | BaUian N Brunieardi FC Wang XP | 2006 | Pancreas2006，33，1： | 1 |
| 12 | Treatment of odontogenic Keratocysts: A fillow-uo 255 chinese patient显示文摘 | Zhao YF Wei JX Wang XP | 2002 | Oral surg Oral Medoral pathol Oral Radial Endod2002，94，2： | 1 |
| 13 | A gold nanorod based colorimetric probe for the rapid and selective detection of Cu~ ions 显示文摘 | Liu JM Wang HF Yan XP | 2011 | Analyst2011，136，19： | 1 |
| 14 | Category and perceptual learningin subjects with treated Wilson’s disease 显示文摘 | Xu P Lu ZL Wang XP | 2010 | PLoS One2010，5，3： | 1 |
| 15 | The pathogenic meehanism of severe acute pancreatitis complicated with renal injury: a review of current knowledge显示文摘 | Zhang XP Wang L Zhou YF | 2008 | Dig Dis Sei2008，53，2： | 1 |
| 16 | MicroRNA - 320 is involved in the regulation of cardiac ischemia/reperfusion injury by targeting heat - shock protein 20 显示文摘 | Ren XP Wu J Wang X | 2009 | Circulation2009，119，17： | 1 |
| 17 | Ultraviolet B exposure of peripheral blood mononuclear ceils of patients with systemiclupus erythematosus inhibits DNA methylation显示文摘 | Wang GS Zhang M Li XP | 2009 | Lupus2009，18，12： | 1 |
| 18 | Finite element analysis of antiimpact loading of wrist protectors显示文摘 | Dong XP Wang DM He JY | 2011 | J Clin Rehab Tiss Eng Res2011，15，30： | 1 |
| 19 | Integrated expression ofthe a-amylase,dextranase and glutathione gene in anindustrial brewer’s yeast strain显示文摘 | Wang JJ Wang ZY He XP | 2012 | World Journal ofMicrobiology and Biotechnology2012，28，1： | 1 |
| 20 | Factors affecting ion-ic liquids based removal of an ionic dyes from water显示文摘 | PEI Y C WANG J J XUAN XP | 2007 | En-viron Sci Technol2007，41，14： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员