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| 1 | Cloning and expression of MXR7 gene in human HCC tissue显示文摘AIM To clone and identify the whole cDNA ofMXR7 gene and to find out its expression inhuman HCC,and normal tissues.METHODS The DNA primers were designed andsynthesized according to the whole cDNAsequence of MXR? gene.The cDNA of humanHCC was taken as the template while the cDNA ofMXR7 gene was synthesized by polymerasechain reaction(PCR).Recombinant DNAconforming to reading frame was constructed byconnecting purified PCR product of the cDNA ofMXR? gene with expression vector pGEX-5X-1 offusion protein.The plasmid MXRT/pGEX-5X-1was identified by sequencing.Using 32p labeledMXR? cDNA as probe,MXR7 mRNA expressionwas detected by Northern blot analysis in 12different human normal tissues,7 preoperativelyuntreated non-liver tumor tissues,30preoperatively untreated HCC,theparacancerous liver tissues and 12 normal livertissues samples.RESULTS Restriction enzyme and sequenceanalysis confirmed that the insertion sequence invector pGEX-5X-1 was the same as the cDNAsequence of MXR7 gene.Northern blot analysisshowed no expression of MXR? mRNA in 12 kindsof normal human tissues including liver,7 tumortissues in other sites and 12 normal livertissues,the frequencies of MXR7 mRNA expression in HCC and paracancerous livertissues were 76.6% and 13.3%,respectively.The frequency of MXR7 mRNA expression in HCCwithout elevation of serum AFP and in HCC<5cm was 90%(9/10)and 83.3%(5/6),respectively.CONCLUSION MXR7 mRNA is highly expressedin human HCC,which is specific and occurs at anearly stage of HCC,suggesting MXR7 mRNA canbe a tumor biomarker for HCC.The detection ofMXR7 mRNA expression in the biopsied livertissue is helpful in discovering early subclinicalliver cancer in those with negative serum AFP. | Zhou XP Wang HY Yang GS Chen ZJ Li BA Wu MC | 2000 | World Journal of Gastroenterology2000，6，1： | 23 |
| 2 | Progress in research of liver surgery in China显示文摘INTRODUCTIONLiver surgery,was started in the late 1950s in Chinaand has developed rapidly in the past 40 years.The study on the diagnosis and treatment of primaryliver cancer in China underwent four stages:①Inthe 1950s,the anatomical study of the liver lay asolid foundation for liver resection.①In | Wu MC Shen F | 2000 | World Journal of Gastroenterology2000，6，6： | 16 |
| 3 | Expression of perforin and granzyme B mRNA in judgement of immunosuppressive effect in rat liver transplantation显示文摘AIM To explore the expression of perform andgranzyme B genes mRNA to judge the effect ofimmunosuppression in acute rejection of livertransplantation.METHODS The expression of perform andgranzyme B genes mRNA was examined byreverse transcriptase-polymerase chain reaction(RT--PCR) in hamster to rat liver grafts under theimmunosuppression of cyclosporine or/andsplenectomy. Histological findings were studiedcomparatively.RESULTS Cyclosporine could obviouslydecrease the cellular infiltration, and completelyrepress the expression of mRNA for perform andgranzyme B, but could not change severehepatocyte necrosis and hemorrhage.SPlenectomy could significantly lightenhepatocyte necrosis, and completely eliminatehemorrhage, but not atfect the cellularinfiltration and the expression of perform andgranzyme B genes mRNA. Cyclosporine orsplenectomy alone could not prolong theSurvival time, however, their combination couldcompletely repress the rejection of liver grafts.The survival time of animals were significantlyprolonged (37.1 days ). The architecture ofhepatic lobules was preserved. There was slightcellular infiltration in the portal tracts and noexpression of perform and granzyme B genesmRNA could be seen in three weeks aftertransplantation.CONCLUSION Perform and granzyme B genesare valuable in judging the effect ofimmunosuppression in liver transplantation. | Zhang SG Wu MC Tan JW Chen H Yang JM Qian QJ | 1999 | World Journal of Gastroenterology1999，5，3： | 15 |
| 4 | Overexpression/amplification of HER-2/neu is uncommon in hepatocellular carcinoma显示文摘 | Xian ZH Zhang SH Cong WM Wu WQ Wu MC | 2005 | 第二军医大学学报2005，26，11： | 6 |
| 5 | Expression of aspartyl beta-hydroxylase and its clinicopathological significance in hepatocellular carcinoma显示文摘 | Xian ZH Zhang SH Cong WM Yan HX Wang K Wu MC | 2006 | 第二军医大学学报2006，27，5： | 6 |
| 6 | Optimal surgical strategy for potentially curable serosa-involved gastric carcinoma with intraperitontoneal free cancer cells 显示文摘 | Wu CC Chen JT Chang MC | 1997 | J Am Coll Surg1997，184，6： | 1 |
| 7 | Molecular basis for antagonism between PDGF and the TGFbeta family of signalling pathways by control of miR-24 expression显示文摘 | Chan MC Hilyard AC Wu C | 2010 | EMBO J2010，29，： | 1 |
| 8 | The deadly connection between endoplasmic retieulum, Ca2+ , protein synthesis, and the endoplasmic reticulum stress response in malignant glioma cells 显示文摘 | Johnson GG White MC Wu JH | 2014 | Neuro Oneol2014，16，8： | 1 |
| 9 | Combination transcatheter hepatic arterial chemoembolization with thymosin alpha 1 on recurrence prevention of hepatocellular carcinoma显示文摘 | Cheng SQ Wu MC Chen H | 2004 | Hepatogastroenterology2004，51，59： | 1 |
| 10 | Proteomic analysis of two functional states of the Golgi complex in mammary epithelial cells显示文摘 | Wu CC Yates JR 3rd Neville MC | | 0，，： | 1 |
| 11 | Influence of nanoclay on properties of HDPE/wood composites显示文摘 | Lei Y Wu QL Craig MC Yao F Xu YJ | | 0，，06： | 1 |
| 12 | Etiology and cytokine expression in patients requiring mechanical ventilation due to severe community-acquired pneumonia 显示文摘 | Wu CL Chan MC Chang GC | 2006 | Formos Med Assoc2006，105，1： | 1 |
| 13 | Clinical features and outcome of coronary artery aneurysm in patients with acute myocardial infarction undergoing a primary percutaneous coronary intervention显示文摘 | Yip HK Chen MC Wu CJ | 2002 | Cardiology2002，98，3： | 1 |
| 14 | Hyperglycemia,blood Pressure,and the 9-year incidence of diabetic retinopathy显示文摘 | Wu SY Hennis A | 2005 | Ophthalmology2005，112，： | 1 |
| 15 | Non-traditional plasticization of polymeric films 显示文摘 | Wu C Mc Ginity | 1999 | lnt J Phar-m1999，177，： | 1 |
| 16 | Mechaniem of reducing platelet activity by percutaneous transluminal mitral valvuloplasty in patients with rheumatic mitral stenosis 显示文摘 | Chen MC Wu CJ Chang HW | 2004 | Chest2004，125，5： | 1 |
| 17 | The largest outbreak of hand,foot and mouth disease in Singapore, in 2008: The role of enterovirus 71 and eoxsaekievirus A strains显示文摘 | Wu Y Yeo A Phoon MC | 2010 | Int J Infect Dis2010，14，12： | 1 |
| 18 | Glutathione-S-transferase M1 regulation of diesel exhaust particle-induced pro-inflammatory mediator expression in normal human bronchial epithelial cells显示文摘 | Wu W Peden D B Mc Connell R | 2012 | Part Fibre Toxicol2012，9，： | 1 |
| 19 | Effect of neonatal isolation on outcome following neonatal sei- zures in rats-the role of eorticosterone 显示文摘 | Lai MC Holmes GL Lee KH Yang SN Wang CA Wu CL | 2006 | Epilepsy Res2006，68，2： | 1 |
| 20 | The largest outbreak of hand foot and mouth disease in Singapore in 2008 : The role of enterovirus 71 and Coxasckievirus A strains 显示文摘 | Wu Y Yeo A Phoon MC | 2010 | Int J Infect Dis2010，14，12： | 1 |
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