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| 1 | Cloning and expression of MXR7 gene in human HCC tissue显示文摘AIM To clone and identify the whole cDNA ofMXR7 gene and to find out its expression inhuman HCC,and normal tissues.METHODS The DNA primers were designed andsynthesized according to the whole cDNAsequence of MXR? gene.The cDNA of humanHCC was taken as the template while the cDNA ofMXR7 gene was synthesized by polymerasechain reaction(PCR).Recombinant DNAconforming to reading frame was constructed byconnecting purified PCR product of the cDNA ofMXR? gene with expression vector pGEX-5X-1 offusion protein.The plasmid MXRT/pGEX-5X-1was identified by sequencing.Using 32p labeledMXR? cDNA as probe,MXR7 mRNA expressionwas detected by Northern blot analysis in 12different human normal tissues,7 preoperativelyuntreated non-liver tumor tissues,30preoperatively untreated HCC,theparacancerous liver tissues and 12 normal livertissues samples.RESULTS Restriction enzyme and sequenceanalysis confirmed that the insertion sequence invector pGEX-5X-1 was the same as the cDNAsequence of MXR7 gene.Northern blot analysisshowed no expression of MXR? mRNA in 12 kindsof normal human tissues including liver,7 tumortissues in other sites and 12 normal livertissues,the frequencies of MXR7 mRNA expression in HCC and paracancerous livertissues were 76.6% and 13.3%,respectively.The frequency of MXR7 mRNA expression in HCCwithout elevation of serum AFP and in HCC<5cm was 90%(9/10)and 83.3%(5/6),respectively.CONCLUSION MXR7 mRNA is highly expressedin human HCC,which is specific and occurs at anearly stage of HCC,suggesting MXR7 mRNA canbe a tumor biomarker for HCC.The detection ofMXR7 mRNA expression in the biopsied livertissue is helpful in discovering early subclinicalliver cancer in those with negative serum AFP. | Zhou XP Wang HY Yang GS Chen ZJ Li BA Wu MC | 2000 | World Journal of Gastroenterology2000，6，1： | 23 |
| 2 | Association of farnesyl diphosphate synthase polymorphisms and response to alendronate treatment in Chinese postmenopausal women with osteoporosis显示文摘 | Liu Y Li M Zhou PR Xing XP Xia WB Xu L Liu H J Zhang ZL LiaoEY Chen DC Liu J Tao TZ Wu W | 2014 | Chinese Medical Journal2014，，4： | 16 |
| 3 | ETB and epidermal growth factor receptor stimulation of wound closure in bovine corneal epithelial cells显示文摘 | Tao WH Gregory IL Wu XP | 1995 | Invest Ophthalmol Vis Sci1995，36，： | 2 |
| 4 | Current study on epidemiology of pancreatic cancer 显示文摘 | WU JX WANG XP XU JY | 2000 | J Clin (in Chinese)2000，17，3： | 2 |
| 5 | A PAT STUDY OF INFLUENCES OF PRECIPITATION ON DEFECTS AND ELECTRONIC DENSITY IN Al-Li-Cu-Mg-Zr ALLOYS显示文摘APATSTUDYOFINFLUENCESOFPRECIPITATIONONDEFECTSANDELECTRONICDENSITYINAlLiCuMgZrALLOYS①WuWeiming,GaoYingjun,LuoLixiong,DengW... | Wu, WM Gao, YJ Luo, LX Deng, W Xu, SJ Zhong, XP Jiang, XJ | 1997 | 中国有色金属学会会刊:英文版1997，7，1： | 2 |
| 6 | Relationship of serum leptinwith age,body weight,body mass index,and bone mineraldensity in healthy mainland Chinese women显示文摘 | Zhong N Wu XP Xu ZR | 2005 | Clin ChimActa2005，351，16： | 1 |
| 7 | Acupuncture for stroke rehabilitation显示文摘 | Wu HM Tang JL Lin XP | 2006 | Cochrane Database Syst Rev2006，，00： | 1 |
| 8 | Long-term outcome of resection of large hepatocellular carcinoma 显示文摘 | Chen XP Qiu FZ Wu ZD | 2006 | Br J Surg2006，93，5： | 1 |
| 9 | MicroRNA - 320 is involved in the regulation of cardiac ischemia/reperfusion injury by targeting heat - shock protein 20 显示文摘 | Ren XP Wu J Wang X | 2009 | Circulation2009，119，17： | 1 |
| 10 | Relationship between age-relat- ed serum concentrations of TGF-betal and TGF-beta2 and those of osteoprotegerin and leptin in native Chinese women 显示文摘 | Zhang N Wu XY Wu XP | 2009 | Clin Chim Acta2009，403，12： | 1 |
| 11 | Relationship of serum leptin with age, body weight, body mass index, and bone mineral density inhealthy mainland Chinese women 显示文摘 | Zhong N Wu XP Xu ZR | 2005 | Clin Chim Acta2005，351，12： | 1 |
| 12 | Chinese experience with hepateetomy for huge hepatoeellular carcinoma显示文摘 | Chen XP Qiu FZ Wu ZD | 2004 | Br J Surg2004，91，3： | 1 |
| 13 | A comparison study of the reference curves of bone mineral density at different skeletal sites in native Chinese, Japanese,and American Caucasian women 显示文摘 | Wu XP Liao EY Huang G | 2003 | Calcif Tissue Int2003，73，： | 1 |
| 14 | Growth hormone downregulated theexpressive apoptosis of ileal intestinal epithelial ceils in rats during theearly course of acute necortizing pancreatitis 显示文摘 | Wang XP Wang BX Wu K | 2002 | Pancreas2002，25，2： | 1 |
| 15 | Oxidized low density lipo- protein receptor-1 mediates oxidized low density lipoprotein-in- duced apoptosis in human umbilical vein endothelial cells: role of reactive oxygen species显示文摘 | CHEN XP XUN KL WU Q | 2007 | Vascul Pharmacol2007，47，1： | 1 |
| 16 | Vaccinationwith peptide mimetics of the gp41 prehairpin fusionintermediate yields neutralizing antisera against HIV-1isolates 显示文摘 | Bianchi E Joyce JG Miller MD Liang XP Finotto MA McKenna P Citron MA Hepler RW Hrin R Nahas D Wu CW Montefiori D Shiver JW Kim PS | 2010 | Proc Natl Acad Sci USA2010，107，10： | 1 |
| 17 | Establishment of BMD reference plots and determination of peak BMD at multiple skeletal regions in mainland Chinese women and the diagnosis of osteoporosis显示文摘 | Wu XP Liao EY Zhang H | 2004 | Osteoporosis Int2004，15，： | 1 |
| 18 | Long-term outcome of resection of large hepatocellular carcinoma显示文摘 | Chen XP Qiu FZ Wu ZD | 2006 | Br J Surg2006，93，5： | 1 |
| 19 | Alpha-methylacyl-CoA racemase/P504S overexpression in colorectal carcinoma is correlated with tumor differentiation显示文摘 | Shi XY Gong EC Wu XP | 2007 | Appl Immunohistochem Mol Morphol2007，15，2： | 1 |
| 20 | Significance of blood circulation disturbance in acute pancreatitis 显示文摘 | Wang XP Wang GL Wu XN etal | 1997 | Journal of New 6astroenterology1997，5，： | 1 |
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