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| 1 | Cloning and expression of MXR7 gene in human HCC tissue显示文摘AIM To clone and identify the whole cDNA ofMXR7 gene and to find out its expression inhuman HCC,and normal tissues.METHODS The DNA primers were designed andsynthesized according to the whole cDNAsequence of MXR? gene.The cDNA of humanHCC was taken as the template while the cDNA ofMXR7 gene was synthesized by polymerasechain reaction(PCR).Recombinant DNAconforming to reading frame was constructed byconnecting purified PCR product of the cDNA ofMXR? gene with expression vector pGEX-5X-1 offusion protein.The plasmid MXRT/pGEX-5X-1was identified by sequencing.Using 32p labeledMXR? cDNA as probe,MXR7 mRNA expressionwas detected by Northern blot analysis in 12different human normal tissues,7 preoperativelyuntreated non-liver tumor tissues,30preoperatively untreated HCC,theparacancerous liver tissues and 12 normal livertissues samples.RESULTS Restriction enzyme and sequenceanalysis confirmed that the insertion sequence invector pGEX-5X-1 was the same as the cDNAsequence of MXR7 gene.Northern blot analysisshowed no expression of MXR? mRNA in 12 kindsof normal human tissues including liver,7 tumortissues in other sites and 12 normal livertissues,the frequencies of MXR7 mRNA expression in HCC and paracancerous livertissues were 76.6% and 13.3%,respectively.The frequency of MXR7 mRNA expression in HCCwithout elevation of serum AFP and in HCC<5cm was 90%(9/10)and 83.3%(5/6),respectively.CONCLUSION MXR7 mRNA is highly expressedin human HCC,which is specific and occurs at anearly stage of HCC,suggesting MXR7 mRNA canbe a tumor biomarker for HCC.The detection ofMXR7 mRNA expression in the biopsied livertissue is helpful in discovering early subclinicalliver cancer in those with negative serum AFP. | Zhou XP Wang HY Yang GS Chen ZJ Li BA Wu MC | 2000 | World Journal of Gastroenterology2000，6，1： | 23 |
| 2 | 门静脉高压并非肝细胞癌肝切除术的绝对禁忌证显示文摘在肝细胞癌(hepatocellular carcinoma,HCC)的各种临床分期系统中,Bruix J团队领衔的巴塞罗那肝癌临床分期标准(Barcelona Clinic Liver Cancer,BCLC)最受推崇,美国和欧洲肝病协会指南的制定亦基于该分期系统。BCLC认为门静脉高压显著增加HCC肝切除术后患者发生肝功能衰竭的风险, | Zhong JH Li H Xiao N Ye XP Ke Y Wang YY Ma L Chen J You XM Zhang ZY Lu SD Li LQ 钟鉴宏 齐鲁楠 | 2014 | 中国癌症防治杂志2014，6，4： | 18 |
| 3 | Association of farnesyl diphosphate synthase polymorphisms and response to alendronate treatment in Chinese postmenopausal women with osteoporosis显示文摘 | Liu Y Li M Zhou PR Xing XP Xia WB Xu L Liu H J Zhang ZL LiaoEY Chen DC Liu J Tao TZ Wu W | 2014 | Chinese Medical Journal2014，，4： | 16 |
| 4 | The role of extracellular signalregulated kinase/mitogen-activated protein kinase pathway in multidrug resistance ofhepatocellular carcinoma显示文摘 | Zhu H Chen XP Luo SF | | 0，，13： | 1 |
| 5 | Long-term outcome of resection of large hepatocellular carcinoma 显示文摘 | Chen XP Qiu FZ Wu ZD | 2006 | Br J Surg2006，93，5： | 1 |
| 6 | MieroRNA-146a and human disease显示文摘 | Li L Chen XP Li YJ | 2010 | Scand J Immunoh2010，71，4： | 1 |
| 7 | Correlation between glucose fluctuation and carotid intima-media thickness in type 2diabetes显示文摘 | Chen XM Zhang Y Shen XP | | 0，，： | 1 |
| 8 | Chinese experience with hepateetomy for huge hepatoeellular carcinoma显示文摘 | Chen XP Qiu FZ Wu ZD | 2004 | Br J Surg2004，91，3： | 1 |
| 9 | Parametric design and application of steering characteristic curve in control for electric power steering显示文摘 | XP Zhao X Li J Chen | 2009 | Mechatronics2009，，19： | 1 |
| 10 | Ginsenoside Rg3 inhibits CXCR4expression and related migrations in a breast cancer cell line显示文摘 | Chen XP Qian LL Jiang H | 2011 | nt JClin Oncol2011，16，5： | 1 |
| 11 | Micro RNA-146a and human disease显示文摘 | Li L Chen XP Li YJ | 2010 | Scand J Immunol2010，71，： | 1 |
| 12 | 2-Methoxyestradiol inhibits hypoxia-indueible factor 1α, tumor growth, and angiogenesis and augments paclitaxel efficary in head and neck squamous cell carcinoma显示文摘 | Ricker JL Chen Z Yang XP | 2004 | Clinical Cancer Res2004，10，24： | 1 |
| 13 | Preparation and identification of monoelonal antibodies against pea albumin Ib(PA Ib)显示文摘 | Wang JH Dun XP Chen ZW | 2005 | Hybridoma2005，24，4： | 1 |
| 14 | Oxidized low density lipo- protein receptor-1 mediates oxidized low density lipoprotein-in- duced apoptosis in human umbilical vein endothelial cells: role of reactive oxygen species显示文摘 | CHEN XP XUN KL WU Q | 2007 | Vascul Pharmacol2007，47，1： | 1 |
| 15 | Extent of liver resection for hilar cholangiocarcinoma显示文摘 | Chen XP Lau WY Huang ZY | | 0，，10： | 1 |
| 16 | Survey of IUD replacing status in Sichuan,PR China显示文摘 | Chen XQ Xie L Pan XP | 2003 | Contraception2003，67，5： | 1 |
| 17 | Extent of liver resection for hilar cholangiocarcinoma显示文摘 | Chen XP Lau WY Huang ZY | 2009 | Br J Surg2009，96，10： | 1 |
| 18 | Dexmedetomidine may benefit cognitive function 'after laparoscopic cholecystectomy in elderly patients显示文摘 | Chen J J Yah JQ Han XP | 2013 | Experimental and Therapeutic Medi- cine2013，5，2： | 1 |
| 19 | Optimized strategy:sequential pegylated interferon α-2a therapy in entecavir-treated patients CHB without satisfactory end-point led to HBeAg seroconversion显示文摘 | Chen XF Chen XP Ma XJ | | 0，，1： | 1 |
| 20 | Correlation between glucose fluctuations and carotid intima-media thickness in type 2 diabetes显示文摘 | CHEN XM ZHANG Y SHEN XP | 2010 | Diabetes Res Clin Pract2010，90，1： | 1 |
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