|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Human umbilical cord mesenchymal stem cell-loaded amniotic membrane for the repair of radial nerve injury显示文摘In this study,we loaded human umbilical cord mesenchymal stem cells onto human amniotic membrane with epithelial cells to prepare nerve conduits,i.e.,a relatively closed nerve regeneration chamber.After neurolysis,the injured radial nerve was enwrapped with the prepared nerve conduit,which was fixed to the epineurium by sutures,with the cell on the inner surface of the conduit.Simultaneously,a 1.0 mL aliquot of human umbilical cord mesenchymal stem cell suspension was injected into the distal and proximal ends of the injured radial nerve with 1.0 cm intervals.A total of1.75×107cells were seeded on the amniotic membrane.In the control group,patients received only neurolysis.At 12 weeks after cell transplantation,more than 80%of patients exhibited obvious improvements in muscular strength,and touch and pain sensations.In contrast,these improvements were observed only in 55–65%of control patients.At 8 and 12 weeks,muscular electrophysiological function in the region dominated by the injured radial nerve was significantly better in the transplantation group than the control group.After cell transplantation,no immunological rejections were observed.These findings suggest that human umbilical cord mesenchymal stem cell-loaded amniotic membrane can be used for the repair of radial nerve injury. | Zhi Li Hanjiao Qin Zishan Feng Wei Liu Ye Zhou Lifeng Yang Wei Zhao Youjun Li | 2013 | Neural Regeneration Research2013，8，36： | 13 |
| 2 | Angiomotin binding-induced activation of Merlin/NF2 in the Hippo pathway显示文摘 | Youjun Li Hao Zhou Fengzhi Li Siew Wee Chan Zhijie Lin Zhiyi Wei Zhou Yang Fusheng Guo Chun Jye Lim Wancai Xing Yuequan Shen Wanjin Hong Jiafu Long Mingjie Zhang | 2015 | Cell Research2015，25，7： | 9 |
| 3 | Evolutionary trace analysis of eukaryotic DNA topoisomerase I superfamily: Identification of novel antitumor drug binding site显示文摘The studies of novel inhibitors of DNA topoisomerase I (Topo I) have already be-come very promising in cancer chemotherapy. Identifying the new drug-binding residues is playing an important role in the design and optimization of Topo I inhibitors. The designed com-pounds may have novel scaffolds, thus will be helpful to overcome the toxicities of current camptothecin (CPT) drugs and may provide a solution to cross resistance with these drugs. Mul-tiple sequence alignments were performed on eukaryotic DNA topoisomerase I superfamily and thus the evolutionary tree was constructed. The Evolutionary Trace method was applied to iden-tify functionally important residues of human Topo I. It has been demonstrated that class-specific hydrophobic residues Ala351, Met428, Pro431 are located around the 7,9-position of CPT, indi-cating suitable substitution of hydrophobic group on CPT will increase antitumor activity. The conservative residue Lys436 in the superfamily is of particular interest and new CPT derivatives designed based on this residue may greatly increase water solubility of such drugs. It has also been demonstrated that the residues Asn352 and Arg364 were conservative in the superfamily, whose mutation will render CPT resistance. As our molecular docking studies demonstrated they did not make any direct interaction with CPT, they are important drug-binding site residues for future design of novel non-camptothecin lead compounds. This work provided a strong basis for the design and synthesis of novel highly potent CPT derivatives and virtual screening for novel lead compounds. | SONG Yunlong QI Yunpeng ZHANG Wannian SHENG Chunquan ZHANG Min YAO Jianzhong YU Jianxin MIAO Zhenyuan ZHOU Youjun ZHU Ju L(U) Jiaguo | 2005 | Science China(Life Sciences)2005，48，4： | 5 |
| 4 | Methylation of RAR-β2, RASSF1A, and CDKN2A Genes Induced by Nickel Subsulfide and Nickel-carcinogenesis in Rats显示文摘Objective To investigate the expression variation of RAR‐β2, RASSF1A, and CDKN2A gene in the process of nickel‐induced carcinogenesis. Methods Nickel subsulfide (Ni 3 S 2 ) at dose of 10 mg was given to Wistar rats by intramuscular injection. The mRNA expression of the three genes in induced tumors and their lung metastasis were examined by Real‐time PCR. The methylation status of the 5' region of these genes were detected by Quantitative Real‐time methylation specific PCR. Results The mRNA expressions of the three genes both in muscle and lung tumor were decreased distinctly in comparison with normal tissue. But hypermethylation was found only in muscle tumor. Conclusion These findings suggest that loss of function or decrease of RAR‐β2, RASSF1A, and CDKN2A, as well as the hypermethylation of 5' region of these genes, are related with nickel exposure. | ZHANG Jun ZHANG Jing LI MengJie Wu YouJun FAN YingYing ZHOU Yang TAN LiLi SHAO ZhiHua SHI HongJun | 2011 | Biomedical and Environmental Sciences2011，24，2： | 4 |
| 5 | RNA interference-mediated silencing of vATPase subunits A and E affect survival and development of the 28-spotted ladybeetle,Henosepilachna vigintioctopunctata显示文摘RNA interference(RNAi)has emerged as a powerful tool for developing novel management strategies for controlling insect pests.The 28-spotted ladybeetle,Henosepilachna vigintioctopunctata is one of the most important pests attacking solanaceous plants in Asia.In this study,the potential of dietary RNAi to manage H.vigintioctopunctata was investigated using both in vitro synthesized and bacterially expressed double-stranded RNAs(dsRNAs)of HvvATPase A and HvvATPase E.The expression levels of HvvATPase A and HvvATPase E were higher in Malpighian tubules than in other tissue types.The silencing of HvvATPase A and HvvATPase E led to significant mortality in H.vigintioctopunctata larvae.In addition,the ingestion of HvvATPase A and HvvATPase E significantly deterred feeding behavior and subsequently arrested the development of H.vigintioctopunctata.Notably,the bacterially expressed dsRNAs consistently caused higher mortality in larvae and adults.Finally,the nontarget effects of the dsRNAs of H.vigintioctopunctata on the predatory ladybeetle Propylaea japonica were evaluated.P.japonica 1st instar larvae were administered vATPase A and vATPase E dsRNAs from H.vigintioctopunctata and P.japonica under the worst-case scenario,in which dsGFP served as negative control.There were significant effects of dsHvvATPase A on P.japonica at the transcriptional level but not at the organismal level,whereas dsHvvATPase E did not effect P.japonica at either the transcriptional or the organismal level.Collectively,the results of the study suggest that HvvATPase A and HvvATPase E can act as novel molecular targets for the control of H.vigintioctopunctata. | Wei Guo Mujuan Guo Chunxiao Yang Zhuoqi Liu Shimin Chen Jing Lu Baoli Qiu Youjun Zhang Xuguo Zhou Huipeng Pan | 2021 | Insect Science2021，28，6： | 3 |
| 6 | In situ time-resolved FTIRS study of adsorption and oxidation of ethylene glycol on Pt(100) electrode显示文摘Adsorption and oxidation of ethylene glyco(EG) on Pt(100) electrode were studied by in situ timeresolved FTIRS (TRFTIRS). The TRFTIR spectra recordedat 0.10 V illustrate that an IR band appears near 2050 cm?at t > 5 s, corresponding to linearly bonded CO formed indissociative adsorption of EG. The TRFTIR results haveconfirmed also that CO species are distributed uniformly onPt(100) surface. Another band appears near 2342 cm?1 at > 70 s, associating with IR absorption of CO2 produced inthe direct oxidation of EG. With the increase of electrodepotential, the direct oxidation of EG becomes gradually themain reaction. When the potential is above 0.40 V, the oxidation of EG occurs mainly via the reactive intermediates, i.especies containing –COOH determined by in situ TRFTIRS. | FAN Youjun ZHOU Zhiyou FAN Chunjie ZHEN Chunhua CHEN Shengpei SUN Shigang | 2005 | Chinese Science Bulletin2005，50，18： | 2 |
| 7 | Spatiotemporal distributions and environmental drivers of diversity and community structure of nosZ-type denitrifiers and anammox bacteria in sediments of the Bohai Sea and North Yellow Sea, China显示文摘Denitrification and anammox processes are major nitrogen removal processes in coastal ecosystems. However, the spatiotemporal dynamics and driving factors of the diversity and community structure of involved functional bacteria have not been well illustrated in coastal environments, especially in human-dominated ecosystems. In this study, we investigated the distributions of denitrifiers and anammox bacteria in the eutrophic Bohai Sea and the northern Yellow Sea of China in May and November of 2012 by constructing clone libraries employing nosZ and 16S rRNA gene biomarkers. The diversity of nosZ-denitrifier was much higher at the coastal sites compared with the central sites, but not significant among basins or seasons. Alphaproteobacteria were predominant and prevalent in the sediments, whereas Betaproteobacteria primarily occurred at the site near the Huanghe (Yellow) River estuary. Anammox bacteria Candidatus Scalindua was predominant in the sediments, and besides, Candidatus Brocadia and Candidatus Kuenenia were also detected at the site near the Huanghe River estuary that received strong riverine and anthropogenic impacts. Salinity was the most important in structuring communities of nosZ-denitrifier and anammox bacteria. Additionally, anthropogenic perturbations (e.g. nitrogen overloading and consequent high primary productivity, and heavy metal discharges) contributed significantly to shaping community structures of denitrifier and anammox bacteria, suggesting that anthropogenic activities would influence and even change the ecological function of coastal ecosystems. | CAI Youjun ZHANG Xiaoli LI Guihao DONG Jun YANG Anjing WANG Guangyu ZHOU Xiaojian | 2019 | Journal of Oceanology and Limnology2019，37，4： | 2 |
| 8 | Effect of powders refinement on the tribological behavior of Ni-based composite coatings by laser cladding显示文摘 | Lingqian Wang Jiansong Zhou Youjun Yu Chun Guo Jianmin Chen | 2012 | Applied Surface Science2012，，17： | 2 |
| 9 | Investigation on compounding and application of C80- C100 high-performance concrete 显示文摘 | YIN Jian ZHOU Shiqiong XIE Youjun | 2002 | Cem Concr Res2002，32，2： | 1 |
| 10 | Investigation on compounding and application of C80–C100 high-performance concrete显示文摘 | Jian Yin Shiqiong Zhou Youjun Xie Yu Chen Qinlian Yan | 2002 | Cement and Concrete Research2002，，2： | 1 |
| 11 | Effects of WC–Ni content on microstructure and wear resistance of laser cladding Ni-based alloys coating显示文摘 | Chun Guo Jianmin Chen Jiansong Zhou Jierong Zhao Lingqian Wang Youjun Yu Huidi Zhou | 2011 | Surface & Coatings Technology2011，，8： | 1 |
| 12 | Impact Analysis of Green Procurement Policy for Forest Products in France and Its Reference显示文摘In order to combat illegal logging and associated trade and promote sustainable forest management, major developed countries, including France, have implemented green procurement policy for forest products. This paper described the background of why France implements green procurement policy for forest products, analyzed the basic content of the policy, and made a comprehensive assessment of the impact of green procurement policy on suppliers, processors, eco-environment and competitiveness of forest products. Finally, with the experiences from French implementation of the green procurement policy for forest products, the paper put forward policy recommendations for China to implement green procurement policy for forest products. | Wang Xiuzhen Chen Jie He Youjun Zhou Dalin | 2011 | Chinese Forestry Science and Technology2011，10，3： | 1 |
| 13 | Let there be light: a bright future for Ca2+ signaling显示文摘Calcium ion(Ca^(2+))is a ubiquitous second messenger in eukaryotic cells.In response to electrical,hormonal,or mechanical stimulation,cytosolic Ca^(2+)levels increase due to Ca^(2+) influxes through ion channels embedded in the plasma membrane or Ca^(2+) releases from intracellular Ca^(2+) stores,such as the endoplasmic | Guolin Ma Jindou Liu Nhung T. Nguyen Yubin Zhou Youjun Wang | 2018 | Science Bulletin2018，63，16： | 1 |
| 14 | Improvement in Resolution of Multiphoton Scanning Structured Illumination Microscopy via Harmonics显示文摘We describe a multiphoton(mP)-structured illumination microscopy(SIM)technique,which demonstrates substantial improvement in image resolution compared with linear SIM due to the nonlinear response of fluorescence.This nonlinear response is caused by the effect of nonsinusoidal structured illumination created by scanning a sinusoidally modulated illumination to excite an mP fluorescence signal.The harmonics of the structured fluorescence illumination are utilised to improve resolution.We present an mP-SIM theory for reconstructing the super-resolution image of the system.Theoretically,the resolution of our m P-SIM is unlimited if all the high-order harmonics of the nonlinear response of fluorescence are considered.Experimentally,we demonstrate an 86 nm lateral resolution for two-photon(2P)-SIM and a 72 nm lateral resolution for second-harmonic-generation(SHG)-SIM.We further demonstrate their application by imaging cells stained with F-actin and collagen fibres in mouse-tail tendon.Our method can be directly used in commercial mP microscopes and requires no specific fluorophores or high-intensity laser. | Lei Wang Xiaomin Zheng Jie Zhou Meiting Wang Jiajie Chen Youjun Zeng Gaixia Xu Ying Wang Haixia Qiu Yonghong Shao Junle Qu Bruce Zhi Gao Ying Gu | 2022 | Engineering2022，，9： | 1 |
| 15 | Optimum mix parametersofhigh-strength self-compacting concrete with ultrapulverized flyash显示文摘 | Youjun Xie Baoju Liu Jian Yin Shiqiong Zhou | 2002 | Cement and Concrete Research2002，32，3： | 1 |
| 16 | Synthesis of oriented zeolite T membranes from clear solutions and their pervaporation properties显示文摘 | Zhou Rongfei Hu Lili Zhang Youjun | | 0，，： | 1 |
| 17 | Some factors affecting early compressive strength of steam-curing concrete with ultrafine fly ash显示文摘 | Liu Baoju Xie Youjun Zhou Shiqiong | 2001 | Cement and Concrete Research2001，31，10： | 1 |
| 18 | Influence of ultrafine fly ash composite on the fluidity and compressive strength of concrete显示文摘 | LIU Baoju XIE Youjun ZHOU Shigiong | 2000 | Cem Concr Res2000，30，9： | 1 |
| 19 | Investigation on compounding and application of C80 - C100 high-performance concrete显示文摘 | YIN Jian ZHOU Shiqiong XIE Youjun | 2002 | Cem Concr Res2002，32，2： | 1 |
| 20 | Preparation, microstructure and tribological behavior of laser cladding NiAl intermetallic compound coatings显示文摘 | Youjun Yu Jiansong Zhou Jianmin Chen Huidi Zhou Chun Guo Lingqian Wang Lianbin Yang | 2011 | Wear2011，，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员