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| 1 | Safety Research in Traditional Chinese Medicine: Methods, Applications, and Outlook显示文摘Traditional Chinese medicine (TCM) is a medical system that has collected and summarized abundant clinical experience over its long history of more than 2000 years. However, the frequent occurrence of TCM-induced adverse reactions has hindered the modernization and internationalization of TCM, while attracting increasing attention from around the world. Unlike chemical drugs and biological agents, the difficulties involved in research on the toxicity and safety of TCM mainly include the complexity of its components and the unpredictability of drug–body interactions. Much of TCM, which has overall therapeutic effects, has the typical mechanisms of multiple components, multiple pathways, and multiple targets. While considering the gradualness and unpredictability of TCM toxicity, the ambiguity of toxicants and safe dosage, and individual differences during long-term TCM administration, we have systematically established key techniques for the toxicity assessment of TCM. These techniques mainly include TCM toxicity discovery in an early phase, based on a combination of drug toxicology genomics and metabolomics;methods to identify dose–toxicity relationships in TCM;and integrated techniques for the exploration of TCM interactions, such as fast-screening tests based on drug-metabolizing enzymes and receptor pathways. In particular, we have developed a new technical system for TCM safety evaluation using molecular toxicology, which has been validated well in research on TCM compatibility contraindication, quality control, and allergen discovery. The application of this key technical platform is introduced here in detail. This application includes model organisms, toxicant biomarkers, a magnetic suspension technique, and the application of network toxicology and computational toxicology in research on the toxicity of Fructus toosendan, Semen cassiae, Polygonum multiflorum, and Fructus psoraleae. | Yue Gao Aihua Liang Xiaohui Fan Limin Hu Feiran Hao Yubo Li | 2019 | Engineering2019，5，1： | 19 |
| 2 | Human umbilical cord-derived mesenchymal stem cell therapy in patients with COVID-19:a phase 1 clinical trial显示文摘No effective drug treatments are available for coronavirus disease 2019(COVID-19).Host-directed therapies targeting the underlying aberrant immune responses leading to pulmonary tissue damage,death,or long-term functional disability in survivors require clinical evaluation.We performed a parallel assigned controlled,non-randomized,phase 1 clinical trial to evaluate the safety of human umbilical cord-derived mesenchymal stem cells(UC-MSCs)infusions in the treatment of patients with moderate and severe COVID-19 pulmonary disease.The study enrolled 18 hospitalized patients with COVID-19(n=9 for each group).The treatment group received three cycles of intravenous infusion of UC-MSCs(3×107 cells per infusion)on days 0,3,and 6.Both groups received standard COVID-treatment regimens.Adverse events,duration of clinical symptoms,laboratory parameters,length of hospitalization,serial chest computed tomography(CT)images,the PaO2/FiO2 ratio,dynamics of cytokines,and IgG and IgM anti-SARS-CoV-2 antibodies were analyzed.No serious UC-MSCs infusion-associated adverse events were observed.Two patients receiving UC-MSCs developed transient facial flushing and fever,and one patient developed transient hypoxia at 12 h post UC-MSCs transfusion.Mechanical ventilation was required in one patient in the treatment group compared with four in the control group.All patients recovered and were discharged.Our data show that intravenous UC-MSCs infusion in patients with moderate and severe COVID-19 is safe and well tolerated.Phase 2/3 randomized,controlled,double-blinded trials with long-term follow-up are needed to evaluate the therapeutic use of UC-MSCs to reduce deaths and improve long-term treatment outcomes in patients with serious COVID-19. | Fanping Meng Ruonan Xu Siyu Wang Zhe Xu Chao Zhang Yuanyuan Li Tao Yang Lei Shi Junliang Fu Tianjun Jiang Lei Huang Peng Zhao Xin Yuan Xing Fan Ji-Yuan Zhang Jinwen Song Dawei Zhang Yanmei Jiao Limin Liu Chunbao Zhou Markus Maeurer Alimuddin Zumla Ming Shi Fu-Sheng Wang | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 16 |
| 3 | Exosome-functionalized polyetheretherketone-based implant with immunomodulatory property for enhancing osseointegration显示文摘The host immune response effecting on biomaterials is critical to determine implant fates and bone regeneration property.Bone marrow stem cells(BMSCs)derived exosomes(Exos)contain multiple biosignal molecules and have been demonstrated to exhibit immunomodulatory functions.Herein,we develop a BMSC-derived Exos-functionalized implant to accelerate bone integration by immunoregulation.BMSC-derived Exos were reversibly incorporated on tannic acid(TA)modified sulfonated polyetheretherketone(SPEEK)via the strong interaction of TA with biomacromolecules.The slowly released Exos from SPEEK can be phagocytosed by co-cultured cells,which could efficiently improve the biocompatibilities of SPEEK.In vitro results showed the Exos loaded SPEEK promoted macrophage M2 polarization via the NF-κB pathway to enhance BMSCs osteogenic differentiation.Further in vivo rat air-pouch model and rat femoral drilling model assessment of Exos loaded SPEEK revealed efficient macrophage M2 polarization,desirable new bone formation,and satisfactory osseointegration.Thus,BMSC-derived Exos-functionalized implant exerted osteoimmunomodulation effect to promote osteogenesis. | Lei Fan Pengfei Guan Cairong Xiao Huiquan Wen Qiyou Wang Can Liu Yian Luo Limin Ma Guoxin Tan Peng Yu Lei Zhou Chengyun Ning | 2021 | Bioactive Materials2021，6，9： | 11 |
| 4 | Trithorax Group Proteins Act Together with a Polycomb Group Protein to Maintain Chromatin Integrity for Epigenetic Silencing during Seed Germination in Arabidopsis显示文摘Polycomb 组(PcG ) 和蛋白质被给看了反对地行动 epigenetically 在优核质调整基因表示的 trithorax 组(trxG ) 。trxG 蛋白质在另外的发展过程在 Arabidopsis,而是他们的角色抵抗调停 PcG 的花的压抑糟糕被理解。我们调查了在 trxG 之间的相互作用基因, TRITHORAX1 (ATX1 ) 的 ARABIDOPSIS 相当或相同的事物和 ULTRAPETALA1 (ULT1 ) ,和 PcG 基因胚胎的花 1 (EMF1 ) 在早开发期间。出人意料地,我们发现在 trxG 基因的变化没能救 emf1 异种的早花的显型。相反, emf1 atx1 ult1 幼苗显示出新奇的肿的根显型和基因表示的巨大的解除管制。在 emf1 atx1 ult1 的规章的基因比在 emf1 单身者异种增加两倍的种子主人的更大的宫外的表示一起显示那 PcG 和 trxG 因素镇压在萌芽以后的种子基因表示。而且,我们发现普遍基因 derepression 与 H3K27me3 的减少的层次被联系, epigenetic 基因表示的压抑的标记,并且与全球性改变的染色质组织。EMF1, ATX1,和 ULT1 能绑种子基因的染色质, ULT1 能身体上与 ATX1 和 EMF1 交往,建议 trxG 和 EMF1 蛋白质直接在为调停 EMF1 的基因 silencing 的目标基因 loci 联系。因此,当 ATX1, ULT1,和 EMF1 反对地交往调整 flowering 时,他们一起工作维持染色质完整并且在萌芽以后阻止早熟的种子基因表示。 | Fan Xu Tony Kuo Yenny Rosli Mao-Sen Liu Limin Wu Long-Fang Oliver Chen Jennifer C. Fletcher Zinmay Renee Sung Li Pu | 2018 | Molecular Plant2018，11，5： | 8 |
| 5 | Water-conserving mining influencing factors identification and weight determination in northwest China显示文摘Water-conserving mining is an effective way to alleviate the contradiction between fragile ecological environment and high-intensity coal mining in the arid and semi-arid region of northwest China. It needs to consider the engineering and geological conditions, hydrogeological conditions and mining methods of coal seams. From the three aspects, this paper systematically analyzes the influencing factors and establishes an identification model with multi-level structures. The model includes three primary factors (including the engineering and geological conditions, hydrogeological conditions and mining methods), nine secondary factors (including overlying strata thickness, aquiclude, mining parameters and etc.), sixteen third-tier factors (including the faults, aquiclude thickness and effective mining height and etc.) and twelve fourth-tier factors (including the fault throw exp on ent, aquiclude permeability and coal pillar sizes and etc.). On the basis, the analytic hierarchy process is used to build the judgment matrix and obtain the weight of each influencing factor. The results indicate that the overlying strata thickness, aquiclude and effective mining height are the most import a nt factors among the primary factors of engineering and geological conditions, hydrogeological conditions and mining methods, respectively. The research results could provide theoretical references for the water-conserving mining of coal resources in northwest China. | Limin Fan Liqiang Ma Yihe Yu Shuokang Wang Yujun Xu | 2019 | International Journal of Coal Science & Technology2019，6，1： | 7 |
| 6 | Community structures and population dynamics of “Candidatus Accumulibacter” in activated sludges of wastewater treatment plants using ppk1 as phylogenetic marker显示文摘Candidatus Accumulibacter has been identified as dominant polyphosphate-accumulating organisms(PAOs) in enhanced biological phosphorus(P) removal(EBPR) from wastewater.This study revealed the relevance of community structure, abundance and seasonal population dynamics of Candidatus Accumulibacter to process operation of wastewater treatment plants(WWTPs) in China using ppk1 gene as phylogenetic marker. All sludge samples had properties of denitrifying P removal using nitrate as an electron acceptor.Accumulibacter abundance in the anaerobic-anoxic-oxic(A^2O) process was the highest(26%of total bacteria), and higher in winter than in summer with a better EBPR performance.Type-II was the dominant Accumulibacter in all processes, and type-I accounted for a small proportion of total Accumulibacter. The abundance of Clade-IIC as the most dominant clade reached 2.59 × 10~9 cells/g MLSS and accounted for 87.3% of total Accumulibacter. Clade IIC mainly contributed to denitrifying P removal. Clades IIA, IIC and IID were found in all processes, while clade-IIF was only found in oxidation ditch process through phylogenetic analysis. High proportion of clade IID to total Accumulibacter led to poor performance of aerobic P-uptake in inverted A^2O process. Therefore, Accumulibacter clades in WWTPs were diverse, and EBPR performance was closely related to the clade-level community structures and abundances of Accumulibacter. | Wei Zeng Limin Zhang Pengchao Fan Jingjing Guo Yongzhen Peng | 2018 | Journal of Environmental Sciences2018，30，5： | 6 |
| 7 | Influence of external electric field on piezotronic effect in ZnO nanowires显示文摘In this work, the piezotronic effect is investigated for the first time in external electric fields ranging from 0 V·cm-I to 2,000 V·cm-1 by using n-type ZnO nanowires supported by a flexible substrate. In the presence of an external electric field, the Schottky barrier height (SBH) is lowered by the image force, allowing more free carriers to pass through the metal-semiconductor junction and enhancing the screening effect on positive piezoelectric polarization charges. As the strength of the external electric field increases, the piezotronic effect is significantly suppressed and the metal-semiconductor contact finally exhibits Ohmic behavior. The experimental results show that devices can be classified into three groups, corresponding to low, moderate, and high carrier densities of the nanowires used. This work not only helps us to explicate the basic physical mechanism of the piezotronic effect in a harsh environment in an electric field but also provides guidelines for future design and fabrication of piezotronic devices. | Fei Xue Limin Zhang Xiaolong Feng Guofeng Hu Feng Ru Fan Xiaonan Wen Li Zheng Zhong Lin Wang | 2015 | Nano Research2015，8，7： | 4 |
| 8 | Interferon as a Mucosal Adjuvant for an Influenza Vaccine in Pigs显示文摘Interferon,a natural protein that is produced by a variety of cells during viral infection,activates the transcription of multiple functional genes in cells,regulates synergy among various signaling pathways,and mediates many biological functions such as antiviral activity,immune regulation,and cell growth.However,clinical research on interferon in livestock is lacking.In this study,recombinant porcine interferon(PoIFNα)was used as an adjuvant,in combination with inactivated influenza virus,to vaccinate 6-week-old pigs via nasal infusion.The transcription of target genes was then monitored and the functions of PoIFNαwere determined with respect to the activation of mucosal immunity.We found that a combination of low-dose PoIFNαand in activated influenza virus could significantly up-regulate the expression of immunoregulatory cytokines such as IL-2,IL-1β,IFN-γ,IL-6,and IL-10 by real-time PCR,suggesting the induction of a strong mucosal innate immune response after administration.In addition,low-dose PoIFNαcan significant enhancing the transcription of genes encoding homing factors including CCR9 and CCR10(P<0.001),thereby resulting in the induction of higher levels of HA-specific antibodies(P<0.05),which can be determined by ELISA and IFA.Post-immunization challenges with H1N1 virus demonstrated that PoIFNot,combined with inactivated influenza virus,could alleviate clinical signs in pigs during the early stages of viral infection.These studies reveal low-dose PoIFNαas a potential mucosal adjuvant for influenza virus in pigs. | Lirong Liu Wenhui Fan He Zhang Shuang Zhang Liang Cui Meng Wang Xiaoyuan Bai Wenxian Yang Lei Sun Limin Yang Wenjun Liu Jing Li | 2019 | Virologica Sinica2019，34，3： | 3 |
| 9 | A new discrete Fourier transform randomness test显示文摘The randomness of random number generators(RNGs) is important for the reliability of cryptographic systems since the outputs of RNGs are usually utilized to construct cryptographic parameters.Statistical tests are employed to evaluate the randomness of the RNG outputs. The discrete Fourier transform(DFT) test is an important test item of the most popular statistical test suite NIST SP800-22. In the standard NIST DFT test and related improved studies, there exist accuracy and efficiency issues. First, the bit sequences generated by known good RNGs have a high probability to be rejected when the sequences are long or the sequence number is large, due to the deviation between the actual distribution of the test statistic values and the assumed normal distribution. Second, the long test time and high memory consumptions of the complex DFT test algorithm also affect its practicability. To solve these problems, we propose a new DFT test method for long sequences(106 or more bits). Different from the previous DFT test methods focusing on making the distribution of the test statistic values closer to the normal distribution, we reconstruct the statistic to follow the chi-square distribution. Our experiment result shows that our method has higher reliability in the two-level test, and could effectively reduce the test time and the memory consumptions.When applying our method on randomness test, the test efficiency has been increased to about 4 times for 106-bit sequences and 7 times for 107-bit sequences. In conclusion, our method has lower probability of making errors, and is more suitable for practical application scenarios. | Meihui CHEN Hua CHEN Limin FAN Shaofeng ZHU Wei XI Dengguo FENG | 2019 | Science China(Information Sciences)2019，62，3： | 3 |
| 10 | A recombinant receptor-binding domain in trimeric form generates protective immunity against SARSCoV-2 infection in nonhuman primates显示文摘A safe and effective vaccine is critical to combat the COVID-19 pandemic.Here,we developed a trimeric SARS-CoV-2 receptor-binding domain(RBD)subunit vaccine candidate that simulates the natural structure of the spike(S)trimer glycoprotein.Immunization with the RBD trimer-induced robust humoral and cellular immune responses,and a high level of neutralizing antibodies was maintained for at least 4.5 months.Moreover,the antibodies that were produced in response to the vaccine effectively cross-neutralized the SARS-CoV-2501Y.V2 variant(B.1.351).Of note,when the vaccine-induced antibodies dropped to a sufficiently low level,only one boost quickly activated the anamnestic immune response,conferring full protection against a SARSCoV-2 challenge in rhesus macaques without typical histopathological changes in the lung tissues.These results demonstrated that the SARS-CoV-2 RBD trimer vaccine candidate is highly immunogenic and safe,providing long-lasting,broad,and significant immunity protection in nonhuman primates,thereby offering an optimal vaccination strategy against COVID-19. | Limin Yang Deyu Tian Jian-bao Han Wenhui Fan Yuan Zhang Yunlong Li Wenqiang Sun Yanqiu Wei Xiaodong Tian Dan-dan Yu Xiao-li Feng Gong Cheng Yuhai Bi Yong-tang Zheng Wenjun Liu | 2021 | The Innovation2021，2，3： | 3 |
| 11 | Fault Analysis on a New Block Cipher DBlock with at Most Two Fault Injections显示文摘DBlock is a new family of block ciphers proposed by Wu et al. in Science China in 2015, which consists of three variants specified as DBlock-128/192/256.DBlock-n employs a 20-round Feistel-type structure with n-bit block size and n-bit key size. We propose the first fault analysis on DBlock and show that no more than 2 pairs of correct/faulty ciphertexts are needed to retrieve the master key. In the attack, a byte-oriented fault is injected in round 16, and three properties including differential distribution of the Sbox, bijection nature of the linear function and Feistel-type key scheduling are fully utilized to distinguish between the correct and wrong keys. A fault position guessing strategy based on known intermediates is adopted, which efficiently makes the known-fault attack apply to the random fault model. The experimental results show that, with a pair of ciphertexts, 11.820-bit exhaustive search is needed to derive the whole 128-bit key on average. With 2 pairs of ciphertexts, the unique key can be determined within 6.5 minutes. | FENG Jingyi CHEN Hua GAO Si CAO Weiqiong FAN Limin ZHU Shaofeng | 2018 | Chinese Journal of Electronics2018，27，6： | 3 |
| 12 | CypA Regulates AIP4-Mediated M1 Ubiquitination of Influenza A Virus显示文摘Cyclophilin A (CypA) is a peptidyl-prolyl cis/trans isomerase that interacts with the matrix protein (M1) of influenza A virus (IAV) and restricts virus replication by regulating the ubiquitin–proteasome-mediated degradation of M1. However,the mechanism by which CypA regulates M1 ubiquitination remains unknown. In this study, we reported that E3 ubiquitin ligase AIP4 promoted K48-linked ubiquitination of M1 at K102 and K104, and accelerated ubiquitin–proteasome-mediated degradation of M1. The recombinant IAV with mutant M1 (K102 R/K104 R) could not be rescued, suggesting that the ubiquitination of M1 at K102/K104 was essential for IAV replication. Furthermore, CypA inhibited AIP4-mediated M1 ubiquitination by impairing the interaction between AIP4 and M1. More importantly, both the mutations of M1 (K102 R/K104 R) and CypA inhibited the nuclear export of M1, indicating that CypA regulates the cellular localization of M1 via inhibition of AIP4-mediated M1 ubiquitination at K102 and K104, which results in the reduced replication of IAV.Collectively, our findings reveal a novel ubiquitination-based mechanism by which CypA regulates the replication of IAV. | Madina Mahesutihan Weinan Zheng Liang Cui Yun Li Pengtao Jiao Wenxian Yang Wei Liu Jing Li Wenhui Fan Limin Yang Wenjun Liu Lei Sun | 2018 | Virologica Sinica2018，33，5： | 3 |
| 13 | Construction of 2D/2D Z-scheme MnO_(2-x)/g-C_(3)N_(4) photocatalyst for efficient nitrogen fixation to ammonia显示文摘Reducing nitrogen to ammonia with solar energy has become a wide concern when it comes to photocatalysis research.It is considered to be one of the more promising alternate options for the conventional Haber-Bosch cycle.Herein,2D g-C_(3)N_(4)composites with modifying ultrathin sheet MnO_(2-x)were prepared and used as nitrogen fixation photocatalyst.With the assistance of the nature of MnO_(2-x),the generation rate of NH_(3)reached 225 mmol g^(-1)h^(-1),which is more than twice over the rate of pristine 2D g-C_(3)N_(4)(107 mmol g^(-1)h^(-1)).The presence of ultrathin sheet MnO_(2-x)shortens the gap of the carriers to the surface of photocatalyst.Thus the speed of electron transfer gets increased.Besides,the construction of Z-scheme heterojunction boosts the separation and migration of photogenerated carriers.As a result,the nitrogen reduction reaction(NRR)performance gets enhanced.The work may provide an example of promoting the NRR performance of non-metallic compound. | Limin Yu Zhao Mo Xianglin Zhu Jiujun Deng Fan Xu Yanhua Song Yuanbin She Huaming Li Hui Xu | 2021 | Green Energy & Environment2021，6，4： | 3 |
| 14 | High-Q, low-mode-volume microsphere-integrated Fabry–Perot cavity for optofluidic lasing applications显示文摘We develop a hybrid optofluidic microcavity by placing a microsphere with a diameter ranging from 1 to 4 μm in liquid-filled plano-plano Fabry–Perot(FP) cavities, which can provide an extremely low effective mode volume down to 0.3–5.1 μm^3 while maintaining a high Q-factor up to 1 × 10~4–5 × 10~4 and a finesse of ~2000. Compared to the pure plano-plano FP cavities that are known to suffer from the lack of mode confinement, diffraction, and geometrical walk-off losses as well as being highly susceptible to mirror misalignment, our microsphere-integrated FP(MIFP) cavities show strong optical confinement in the lateral direction with a tight mode radius of only 0.4–0.9 μm and high tolerance to mirror misalignment as large as 2°. With the microsphere serving as a waveguide, the MIFP is advantageous over a fiber-sandwiched FP cavity due to the open-cavity design for analytes/liquids to interact strongly with the resonant mode, the ease of assembly, and the possibility to replace the microsphere. In this work, the main characteristics of the MIFP, including Q-factor, finesse, effective mode radius and volume, and their dependence on the surrounding medium's refractive index, mirror spacing, microsphere position inside the FP cavity, and mirror misalignment, are systematically investigated using a finite-element method.Then, by inserting dye-doped polystyrene microspheres of various sizes into the FP cavity filled with water, we experimentally realize single-mode MIFP optofluidic lasers that have a lasing threshold as low as a few microjoules per square millimeter and a lasing spot radius of only ~0.5 μm. Our results suggest that the MIFP cavities provide a promising technology platform for novel photonic devices and biological/chemical detection with ultra-small detection volumes. | XIAOQIN WU YIPEI WANG QIUSHU CHEN YU-CHENG CHEN XUZHOU LI LIMIN TONG XUDONG FAN | 2019 | Photonics Research2019，7，1： | 3 |
| 15 | The self-assembled nanoparticle-based trimeric RBD mRNA vaccine elicits robust and durable protective immunity against SARS-CoV-2 in mice显示文摘As COVID-19 continues to spread rapidly worldwide and variants continue to emerge,the development and deployment of safe and effective vaccines are urgently needed.Here,we developed an mRNA vaccine based on the trimeric receptor-binding domain(RBD)of the SARS-CoV-2 spike(S)protein fused to ferritin-formed nanoparticles(TF-RBD).Compared to the trimeric form of the RBD mRNA vaccine(T-RBD),TF-RBD delivered intramuscularly elicited robust and durable humoral immunity as well as a Th 1-biased cellular response.After further challenge with live SARS-CoV-2,immunization with a two-shot low-dose regimen of TF-RBD provided adequate protection in hACE2-transduced mice.In addition,the mRNA template of TF-RBD was easily and quickly engineered into a variant vaccine to address SARS-CoV-2 mutations.The TF-RBD multivalent vaccine produced broad-spectrum neutralizing antibodies against Alpha(B.1.1.7)and Beta(B.1.351)variants.This mRNA vaccine based on the encoded self-assembled nanoparticle-based trimer RBD provides a referenee for the design of mRNA vaccines targeting SARS-CoV-2. | Wenqiang Sun Lihong He He Zhang Xiaodong Tian Zhihua Bai Lei Sun Limin Yang Xiaojuan Jia Yuhai Bi Tingrong Luo Gong Cheng Wenhui Fan Wenjun Liu Jing Li | 2021 | Signal Transduction and Targeted Therapy2021，6，10： | 3 |
| 16 | Sigmoidocolocystoplasty With Ureteral Reimplantation for Treatment of Neurogenic Bladder显示文摘 | Fan Zhang Limin Liao | 2012 | Urology2012，，2： | 2 |
| 17 | Geological Evidence for the Presence of the Emperor Event in China显示文摘The presence of the reversals of the geomagnetic field within the Brunhes is indicated by paleomagnetic stuides on Cenozoic volcanic rocks in the Leizhou Peninsula, loess strata and Quaternary marine sediments in the Pearl River Mouth basin in China. These regional reversals occured at 0.58±0.13 Ma, 0.45 Ma and 0.47—0.48 Ma respectively according to the results of K-Ar isotopic dating and climatostratigraphic and biostratigraphic analyses. The reversals equivalent to the Emperor Event is suggested.The reversals revealed in different regions with various lithologic characters in China prove that the Emperor Event is a global reversal event rather than a geomagnetic excursion, and its confirmation will permit a precise definition of the Middle/Late Pleistocene boundary. | Ge Tongming, Fan Limin and Liu Jian Guangzhou Marine Geological Survey, Ministry of Geology and Mineral Resources of China, Guangzhou | 1993 | Acta Geologica Sinica(English Edition)1993，67，2： | 2 |
| 18 | A review on investigation of water-preserved coal mining in western China显示文摘Yushenfu mining area is located in an ecological fragile area in western China, the coal seam of which is the Jurassic Ysn'an Foirnalion. The Jurassic Yan'an Formation con tains five minable coal seams, the top layer of which is thick, covered by shallow overburden and located under aquifers. Therefore, the mining induced water flowing fractured zone can easily extend to the aquifers of both the Quaternary Sarahu and Jurassic Zhiluo Formation. This would result in a series of negative hydrological and ecological effects, including groundwater leakage, groundwater lowering, furtherly causing surface vegetation withering and dying, surface water body reduction, spring drying out, and water flow of river being decreased substantially. To solve these environmental problems, several technologies have been carried out by Chinese scientists, one of which is water-preserved coal mining. This paper presents a review of the origin, definition and development of water-preserved coal mining, and its applications in Yushenfu mining area. The applicable conditions, research contents, research methodology, and technical foundation of water-preserved coal mining are addressed in this paper. The future research focuses regarding water-preserved coal mining in China are also discussed in this paper. Its results serve as a guide for selecting the methods to be preferred for mining in case the geological conditions, roof overburden structure and coal mining process are similar to Yushenfu mining area. | Limin Fan Xiongde Ma | 2018 | International Journal of Coal Science & Technology2018，5，4： | 2 |
| 19 | Insights into salvianolic acid B biosynthesis from chromosome-scale assembly of the Salvia bowleyana genome显示文摘Salvia bowleyanais a traditional Chinese medic-inal plant that is a source of nutritional supple-ments rich in salvianolic acid B and a potentialexperimental system for the exploration ofsalvianolic acid B biosynthesis in the Labiatae.Here,we report a high‐quality chromosome‐scalegenome assembly of S.bowleyana covering 462.44 Mb,with a scaffold N50 value of 57.96 Mband 44,044 annotated protein‐coding genes.Evolutionary analysis revealed an estimateddivergence time betweenS.bowleyanaand its close relativeS.miltiorrhiza of~3.94 millionyears.We also observed evidence of a whole‐genome duplication in theS.bowleyanagenome.Transcriptome analysis showed that SbPAL1(PHENYLALANINE AMMONIA‐LYASE1)ishighlyexpressed in roots relative to stem and leaves,paralleling the location of salvianolic acid Baccumulation.The laccase gene family in S.bowley anaoutnumbered their counterparts inbothS.miltiorrhiza and Arabidopsis thaliana,suggesting that the gene family has undergoneexpansion inS.bowleyana.Several laccasegenes were also highly expressed in roots,wheretheir encoded proteins may catalyze the oxida-tive reaction from rosmarinic acid to salvianolicacid B.Thesefindings provide an invaluable ge-nomic resource for understanding salvianolicacid B biosynthesis and its regulation,and will beuseful for exploring the evolution of the Labiatae. | Xuehai Zheng Duo Chen Binghua Chen Limin Liang Zhen Huang Wenfang Fan Jiannan Chen Wenjin He Huibin Chen Luqiang Huang Youqiang Chen Jinmao Zhu Ting Xue | 2021 | Journal of Integrative Plant Biology2021，63，7： | 2 |
| 20 | The chemical chaperon 4-phenylbutyric acid ameliorates hepatic steatosisthrough inhibition of de novo lipogenesis in high-fructose-fed rats显示文摘 | Lu-Ping Ren Guang-Yao Song Zhi-Juan Hu Mingming Zhang Lanbo Peng Shu-Chun Chen Limin Wei Fan Li Wen Sun | 2013 | International Journal of Molecular Medicine2013，，： | 2 |
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