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| 1 | Establishment and evaluation of a peanut association panel and analysis of key nutritional traits显示文摘Breeding programs aim to improve the yield and quality of peanut(Arachis hypogaea L.); using association mapping to identify genetic markers linked to these quantitative traits could facilitate selection efficiency. A peanut association panel was established consisting of 268 lines with extensive phenotypic and genetic variation, meeting the requirements for association analysis. These lines were grown over 3 years and the key agronomic traits, including protein and oil content were examined. Population structure(Q) analysis showed two subpopulations and clustering analysis was consistent with Q-based membership assignment and closely related to botanical type. Relative Kinship(K) indicated that most of the panel members have no or weak familial relatedness, with 52.78% of lines showing K ? 0. Linkagedisequilibrium(LD) analysis showed a high level of LD occurs in the panel. Model comparisons indicated false positives can be effectively controlled by taking Q and K into consideration and more false positives were generated by K than Q. A preliminary association analysis using a Q t K model found markers significantly associated with oil, protein, oleic acid, and linoleic acid, and identified a set of alleles with positive and negative effects. These results show that this panel is suitable for association analysis,providing a resource for marker-assisted selection for peanut improvement. | Xiurong Zhang Suqing Zhu Kun Zhang Yongshan Wan Fengzhen Liu Qingfang Sun Yingjie Li | 2018 | Journal of Integrative Plant Biology2018，60，3： | 5 |
| 2 | Molecular analysis of the chloroplast Cu/Zn-SOD gene(AhCSD2) in peanut显示文摘Superoxide dismutase(SOD, EC 1.15.1.1) plays a key role in response to drought stress, and differences in SOD activity changes among cultivars are important under drought conditions. We obtained the full-length DNA of the chloroplast Cu/Zn-SOD gene(Ah CSD2)from 11 allotetraploid cultivars and 5 diploid wild species in peanut. BLAST search against the peanut genome showed that the Ah CSD2 genes g CSD2-1 and g CSD2-2 are located at the tops of chromosome A03(A genome) and B03(B genome), respectively, and both contain 8exons and 7 introns. Nucleotide sequence analyses indicated that g CSD2-2 sequences were identical among all the tested cultivars, while g CSD2-1 sequences showed allelic variations.The amino acid sequences deduced from g CSD2-1 and g CSD2-2 both contain a chloroplast transit peptide and are distinguished by 6 amino acid(aa) residue differences. The other 2aa residue variations in the mature peptide regions give rise to three-dimensional structure changes of the protein deduced from the genes g CSD2-1 and g CSD2-2. Sequences analyses of cultivars and wild species showed that g CSD2-2 of Arachis hypogaea and g Aip CSD2(Arachis ipaensis) are identical, and despite the abundant polymorphic loci between g CSD2-1 of A.hypogaea and sequences from A genome wild species, the deduced amino acid sequence of Ah CSD2-1(A. hypogaea) is identical to that of Adu CSD2(Arachis duranensis), whereas Aco CSD2(Arachis correntina) and Aca CSD2(Arachis cardenasii) both have 2 aa differences in the transit peptide region compared with Ah CSD2-1(A. hypogaea). Based on the Peanut Genome Project, promoter prediction revealed many stress-related cis-acting elements within the potential promoter regions(pp-A and pp-B). pp-A contains more binding sites for drought-associated transcriptional factors than pp-B. We hypothesize that the marked changes in SOD activity in different cultivars under drought stress are tightly regulated by transcription factors through transcription and expression of Ah CSD2 genes. | Xiurong Zhang Qian Wan Fengzhen Liu Kun Zhang Aiqing Sun Bing Luo Li Sun Yongshan Wan | 2015 | The Crop Journal2015，3，3： | 4 |
| 3 | Erosive Wear and Wear Mechanism of in situ TiC_P/Fe Composites显示文摘The base structure of in situ TiCp/Fe composites fabricated under industrial condition was changed by differentheat treatments. Erosive wear tests were carried out and the results were compared with that of wear-resistant whitecast iron. The results suggest that the wear resistance of the in situ TiCP/Fe composite is higher than that ofwear-resistant white cast iron under the sand erosive wear condition. The wear mechanism of the wear-resistantwhite cast iron was a cycle process that base surface was worn and carbides were exposed, then carbides was brokenand wear pits appeared. While the wear mechanism of in situ TiCP/Fe composite was a cycle process that basesurface was worn and TiC grains were exposed and dropped. The wear resistance of in situ TiCP/Fe composite waslower than that of wear-resistant white cast iron under the slurry erosive wear condition. Under such circumstance,the material was not only undergone erosive wear but also electrochemistry erosion due to the contact with waterin the medium. The wear behaviours can be a combination of two kinds of wear and the sand erosive wear is worsethan slurry erosive wear. | Zhaojing LIU, Zhiliang NING , Fengzhen LI, Xiurong YAO and Shanzhi RENSchool of Materials Science and Engineering, Harbin University of Science and Technology, Harbin 150080, China | 2005 | Journal of Materials Science & Technology2005，21，5： | 3 |
| 4 | Determining the critical size of a rabbit rib segmental bone defect model显示文摘In order to establish and standardize the rabbit rib segmental bone defect model,it is of vital importance to determine rabbit rib critical size defect(CSD).According to the general time needed for spontaneous long-bone regeneration,three-month observation period was set to determine the CSD.The rabbit rib segmental bone defects with different sizes from 1 to 5cm with or without periosteum were performed in the eighth rib of 4-month-old male New Zealand rabbits and underwent Xray examinations at the 4th,8th and 12th weeks postoperatively.The gross and histological examinations at postoperative week 12 were evaluated,which showed that the critical sizes in the rabbit rib models with and without periosteum were 5 and 2 cm,respectively.This study provides prerequisite data for establishing rabbit rib CSD model and evaluating bonematerials using this model. | Fengzhen Liu Kun Chen Lei Hou Keyi Li Dawei Wang Bin Zhang Xiumei Wang | 2016 | Regenerative Biomaterials2016，3，5： | 2 |
| 5 | Dynamics and manipulation of ferroelectric domain walls in bismuth ferrite thin films显示文摘Ferroelectric domain walls differ from domains not only in their crystalline and discrete symmetry,but also in their electronic,magnetic,and mechanical properties.Although domain walls provide a degree of freedom to regulate the physical properties at the nanoscale;the relatively lower controllability prevents their practical applications in nano-devices.In this work;with the advantages of 3D domain configuration detection based on piezoresponse force microscopy;we find that the mobility of three types of domain walls(tail-to-tail,head-to-tail,head-to-head)in(001)BiFeO3 films varies with the applied electrical field.Under low voltages;head-to-tail domain walls are more mobile than other domain walls;while,under high voltages,tail-to-tail domain walls become rather active and possess relatively long average lengths.This is due to the high nucleation energy and relatively low growth energy for charged domain walls.Finally;we demonstrate the manipulation of domain walls through successive electric writings,resulting in well-aligned conduction paths as designed;paving the way for their application in advanced spintronic;memory and communication nano-devices. | Shuyu Xiao Yaming Jin Xiaomei Lu Sang-Wook Cheong Jiangyu Li Yang Li Fengzhen Huang Jinsong Zhu | 2020 | National Science Review2020，7，2： | 2 |
| 6 | Multiscale ApEn and SampEn in Quantifying Nonlinear Complexity of Depressed MEG显示文摘Depression is a neurophysiological disorder with recurrent dysregulations of self-mental states.Multiscale Approximate entropy(ApEn)and Sample entropy(SampEn)are employed to characterize nonlinear complexity of Magnetoencephalography(MEG)of depressive patients in our contribution.SampEn shares similarities with ApEn while has better distinctions between the MEGs of depression patients and normal people.Test results prove that nonlinear complexity of the depressive MEG is lower than that of the normal subjects,indicating weaker response of depression patients to emotional stimuli,and the optimum discriminations between the depressive and healthy people lie in frontal lobe of brain which is related to emotional regulation.Our findings provide valuable information about depression,highlight the loss of nonlinear complexity in MEG of depressive patient and can be used as clinical diagnostic aids. | YAO Wenpo HU Hui WANG Jun YAN Wei LI Jin HOU Fengzhen | 2019 | Chinese Journal of Electronics2019，28，4： | 2 |
| 7 | The APOE ε2 allele may decrease the age at onset in patients with spinocerebellar ataxia type 3 or Machado-Joseph disease from the Chinese Han population显示文摘 | Huirong Peng Chunrong Wang Zhao Chen Zhanfang Sun Bin Jiao Kai Li Fengzhen Huang Xuan Hou Junling Wang Lu Shen Kun Xia Beisha Tang Hong Jiang | 2014 | Neurobiology of Aging2014，，9： | 1 |
| 8 | Preparation, optimization, and characterization of chitosancoated solid lipid nanoparticles for ocular drug delivery显示文摘The present study aimed to develop and optimize chitosan coated solid lipid nanoparticles(chitosan-SLNs)encapsulated with methazolamide. Chitosan-SLNs were successfully prepared by a modified oil-in-water emulsification-solvent evaporation method with glyceryl monostearate as the solid lipid and phospholipid as the surfactant. Systematic screening of formulation factors was carried out. The optimized formula for preparation was screened by orthogonal design as well as Box-Behnken design with entrapment efficiency, particle size and zeta potential as the indexes. The entrapment efficiency of the optimized formulation(methazolamide-chitosan-SLNs)prepared was(58.5± 4.5)%,Particle size(247.7 ± 17.3) nm and zeta potential(33.5 ±3.9) mV. Transmission electron microscopy showed homogeneous spherical particles in the nanometer range. A prolonged methazolamide in vitro release profile was obtained in the optimized chitosan-SLNs suspension compared with methazolamide solution. No ocular damages were observed in the susceptibility test on albino rabbits. The results suggest that the combination of orthogonal design and Box-Behnken design is efficient and reliable in the optimization of nanocarriers, and chitosanSLNs is a potential carrier for ophthalmic administration. | Fengzhen Wang Mingwan Zhang Dongsheng Zhang Yuan Huang Li Chen Sunmin Jiang Kun Shi Rui Li | 2018 | The Journal of Biomedical Research2018，32，6： | 1 |
| 9 | High rate deposition of microcrystalline silicon films by high-pressure radio frequency plasma enhanced chemical vapor deposition (PECVD)显示文摘Hydrogenated microcrystalline silicon (μc-Si:H) thin films were prepared by high- pressure radio-frequency (13.56 MHz) plasma enhanced chemical vapor deposition (rf-PECVD) with a screened plasma. The deposition rate and crystallinity varying with the deposition pressure, rf power, hydrogen dilution ratio and electrodes distance were systematically studied. By optimizing the deposition parameters the device quality μc-Si:H films have been achieved with a high deposition rate of 7.8 /s at a high pressure. The Voc of 560 mV and the FF of 0.70 have been achieved for a single-junction μc-Si:H p-i-n solar cell at a deposition rate of 7.8 /s. | ZHOU BingQing ZHU MeiFang LIU FengZhen LIU JinLong ZHOU YuQin LI GuoHua DING Kun | 2008 | Science China(Technological Sciences)2008，51，4： | 1 |
| 10 | CDCP:a visualization and analyzing platform for single-cell datasets显示文摘Recently,the rapid advances of single-cell sequencing technologies,including sequencing of single-cell genomics,transcriptomes,epigenetics,and spatial transcriptomes,have empowered researchers to scrutinize cellular heterogeneity,gene expression,epigenetic modifications. | Yuejiao Li Tao Yang Tingting Lai Lijin You Fan Yang Jiaying Qiu Lina Wang Wensi Du Cong Hua Zhicheng Xu Jia Cai Zhiyong Li Yiqun Liu Ling Li Minwen Zhang Jing Chen Lei Zhang Dongsheng Chen Weiwen Wang Shiping Liu Liang Wu Wenjun Zeng Bo Wang Xiaofeng Wei Longqi Liu Fengzhen Chen | 2022 | Journal of Genetics and Genomics2022，49，7： | 0 |
| 11 | Thin Layer Identification of Jiedu Shengxue Granules and Determination of Notoginsenoside R1 Content显示文摘[Objectives]To establish the quality standard of hospital preparation Jiedu Shengxue granules.[Methods]Scleromitrion diffusum and Prunella vulgaris in Jiedu Shengxue granules were qualitatively identified by thin layer chromatography(TLC).A high performance liquid chromatography(HPLC)was established to determine the content of notoginsenoside R1 in the granule.[Results]The traditional Chinese medicinal materials in Jiedu Shengxue granules could be identified by TLC,and the characteristic spots were stable and clear.Notoginsenoside R1 had a good linear relationship in the range of 10.45-104.5μg/mL,with an average recovery of 98.52%and RSD=2.36%.[Conclusions]TLC and HPLC,as the quality control methods of Jiedu Shengxue granules,have high accuracy and good repeatability,which lays a foundation for the quality control of this mixture. | Zhenying FU Bing QING Yinghong HUANG Xianyi SHI Meiyan QIU Xian PENG Jiangcun WEI Fengzhen LI Wen ZHONG | 2024 | Medicinal Plant2024，15，1： | 0 |
| 12 | MECHANISM OF Re-B-V CO-PERMEATION显示文摘MECHANISMOFReBVCOPERMEATION①LiFengzhen,LiuZhaojing,JinQuan,XuZhihuiDepartmentofMaterialsScienceandEngineering,HarbinUniver... | Li, Fengzhen Liu, Zhaojing Jin, Quan Xu, Zhihui | 1997 | 中国有色金属学会会刊:英文版1997，7，1： | 0 |
| 13 | Pathogenicity of Cell Wall Degrading Enzymes Produced by Botryodiplodia theobromae Pat. against Mangoes显示文摘[Objective]This study aimed to confirm the roles of cell wall degrading enzymes (CWDEs) produced by Botryodiplodia theobromae Pat.in the infection of mango fruits. [Method] Change of activities of five types of CWDEs produced by B. theobromae Pat.were studied under both in vitro culture and inoculation conditions,along with the pathogenicity and the ability of producing CWDEs of four post-harvest fungal pathogens(B. theobromae Pat.,Colletotrichum gloeosporioides Penz. ,Phomopsis mangiferae Ahmad and Dothiorella dominicana Pet. et Cif.) which cause stem-end rot of mangoes. [Result]B. theobromae Pat. was able to produce polygalacturonase(PG),pectinmethylgalacturonase(PMG),polygalacturonic acid trans-eliminase (PGTE),pectin methyltrans-eliminase (PMTE) and cellulase (Cx.) under both in vitro culture and inoculation conditions,of which activities of PG,Cx and PMG were significantly higher in than that in either PGTE or PMTE. Among three primary CWDEs,the peak of activities of PG and Cx appeared earlier and that of PMG occured later. The pathogenicity of B. theobromae Pat. was significantly higher than that of any other three pathogens;it is the same with the abilities of producing pectinase.[Conclusion]This paper provides theoretical bases for further exploring the mechanism of host-pathogen interaction and decreasing the post-harvest loss of mango fruits. | Min LI Zhaoyin GAO Meijiao HU Shuo ZHOU Dongping YANG Bo YANG Jianxue YI Fengzhen YANG | 2012 | Agricultural Biotechnology2012，1，6： | 0 |
| 14 | Integrin α_(v)β_(3)-targeted polydopamine-coated gold nanostars for photothermal ablation therapy of hepatocellular carcinoma显示文摘Photothermal therapy(PTT)has emerged as a promising cancer therapeutic method.In this study,Arg-Gly-Asp(RGD)peptide-conjugated polydopamine-coated gold nanostars(Au@PDA-RGD NPs)were prepared for targeting PTT of hepatocellular carcinoma(HCC).A polydopamine(PDA)shell was coated on the surface of gold nanostars by the oxidative self-polymerization of dopamine(termed as Au@PDA NPs).Au@PDA NPs were further functionalized with polyethylene glycol and RGD peptide to improve biocompatibility as well as selectivity toward the HCC cells.Au@PDARGD NPs showed an intense absorption at 822 nm,which makes them suitable for near-infraredexcited PTT.Our results indicated that the Au@PDA-RGD NPs were effective for the PTT therapy of the α_(v)β_(3) integrin receptor-overexpressed HepG2 cells in vitro.Further antitumor mechanism studies showed that the Au@PDA-RGD NPs-based PTT induced human liver cancer cells death via the mitochondrial-lysosomal and autophagy pathways.In vivo experiments showed that Au@PDARGD NPs had excellent tumor treatment efficiency and negligible side effects.Thus,our study showed that Au@PDA-RGD NPs could offer an excellent nanoplatform for PTT of HCC. | Yang Li Ping Hu Xiali Wang Xu Hou Fengzhen Liu Xiaohong Jiang | 2021 | Regenerative Biomaterials2021，8，5： | 0 |
| 15 | Analysis and Verification of the Conserved MYB Binding Element in the DFR Promoter in Compositae显示文摘Anthocyanins,ubiquitous in the Compositae family,are regulated by MYB(v-myb avian myeloblastosis viral oncogene homolog),playing an important role in anthocyanin synthesis.In this study,we analyzed the regulation pathway in which the MYB protein of subgroup 6 promotes dihydroflavonol reductase(DFR)expression in Compositae,and validated this law in Saussurea medusa through yeast one-hybrid experiments.Our results showed that MYB and DFR underwent purification selection,DFR promoter analysis revealed the presence of MYB binding site(GAGTTGAATGG)and bHLH binding site(CANNTG)at the sense strand of 84–116 nucleotide residues from the start codon.These two motifs were separated by 9–10 nucleotide residues,as existed in the DFR promoters of many Compositae plants.Furthermore,the yeast one-hybrid experiment demonstrated that SmMYB1 can activate the promoter of SmDFR.Our results provide a reference for further functional study of DFR in Compositae. | Jialei Guo Fengzhen Li Guomin Shi Weimin Zhao Tao He | 2024 | Phyton-International Journal of Experimental Botany2024，93，2： | 0 |
| 16 | Zwitterionic ring-opening polymerization of macrocyclic ethyleneoxy-substituted carbonate:Access to cyclic PEG-like polycarbonate显示文摘The innovation in polymer design to rival conventional polyethylene glycol(PEG)is an important approach to achieving a more sustainable society.Here,cyclic PEG-like polycarbonates having high molecular weight(4.4–49.5 kg/mol)were enabled through zwitterionic ring-opening polymerization(ZROP)of macrocyclic carbonates(MCs)mediated by N-heterocyclic carbene(NHC).The thermodynamic behavior of polymerization depends on the ring size of monomers.During this process,the ZROP of 11-membered MC was driven by the change of enthalpy(ΔH_(p))which differed from the ZROP of 14-membered MC driven by the entropic change(ΔS_(p)).Cyclic polycarbonates depicted improved thermostability(T_(d5%)≥204℃)and higher glass transition temperatures(T_(g)>–40℃)in comparison to their linear analogues(T_(d5%)≤185℃,T_(g)~–50℃).In addition,the mechanism of ZROP of MC was addressed through computational study.A distinct mechanism of polymerization distinguishable from the well-known NHC-mediated ZROP of cyclic esters was revealed,where the zwitterion from nucleophilic addition to MC,i.e.tetrahedral intermediate,cannot be ring-opened probably due to the delocalization of negative charge on the carbonate group,but serves as an active center for the polymerization.In comparison to PEG,the attained polymer demonstrated comparable hydrophilic and biocompatible properties,as revealed by the results of contact angle and in vitro cytotoxicity studies,suggesting that cyclic polycarbonate hold the promise as the alternative of PEG. | Jin Huang Jinwen Li Rui Yan Yuanyuan Qu Fengzhen Guo Lei Shen Can-liang Ma Jie Sun Zhenjiang Li Kai Guo | 2024 | Chinese Chemical Letters2024，35，3： | 0 |
| 17 | Comparative Transcriptome Analysis Reveals Different Mechanisms of Adaptation to Environment among Three Species of Saussurea DC.显示文摘Saussurea medusa,Saussurea hypsipeta and Saussurea obvallata are typical alpine snowline plants growing in the Qinghai-Tibet plateau.They are characterized by a specialized morphology.S.medusa and S.hypsipeta have very dense trichomes on whole plant,whereas S.obvallata has transparent bracts covered inflorescences.The different forms reflect their adaptation to cold environments.To investigate the different mechanisms of adaptation of these species to cold temperatures,transcriptome sequencing was performed in three species of Saussurea DC.A total of 116394137237 and 113879 Unigenes were identified from S.medusa,S.hypsipeta and S.obvallata,respectively.Of these,55909(48.03%),65519(47.74%)and 51889(45.56%)Unigenes were matched in public databases.GO analysis identified that most of annotated Unigenes in the three species of plants were related to cellular,metabolic,and single−organism processes,and binding and catalytic activities.The differential expression of 37 genes related to environmental adaptation were discovered by pairwise comparisons.Of these,two candidate genes(Interaptin-like and CSLB3)related to trichome development were identified only in S.medusa and S.hypsipeta,which was consistent with their distinct morphology.Our data can provide a valuable resource for the further studies on the adaptive mechanisms of molecular and functional ecology in Saussurea DC. | Weimin Zhao Fengzhen Li Guomin Shi Jialei Guo Guifang He Tao He | 2022 | Phyton-International Journal of Experimental Botany2022，91，7： | 0 |
| 18 | Study on Mature Age of Individual Larix kaempferi显示文摘Based on the study of parse wood materials,the fitting empirical equation of tree growth was obtained,a function with tree growth as a variable and time as an independent variable.Through mathematical operations such as function derivation,the mature age of tree growth was obtained,and the obtained expected mature age for Larix kaempferi was 46 years.And the application,research directions and precautions of the mature ages were proposed. | Keqin LI Lingling LI Fengzhen WANG Dequan ZHANG Dongmei YUE Keqing FANG | 2019 | Agricultural Biotechnology2019，8，3： | 0 |
| 19 | Estimation of target depth using deep-sea echo multipath structure matching显示文摘This paper presents a method to estimate the depth of deep-sea target by matching the time delay and amplitude structure of echo multipath highlight clusters.The echo structure is the convolution of two-way channel transfer function as well as the acoustic scattering function of the target,which shows the characteristics of deep-sea highlight clusters when the target is close to the sea surface or near the sea bottom.The time delay and amplitude structure of highlight clusters are related to the target depth,which can be used to estimate the target depth.A method of matching estimation of target depth on range-depth grid using nonlinear programming cost function is established.The mismatch effects of inter-cluster time delay interval deviation and sea water sound velocity profile deviation on matching estimation are simulated and analyzed.The results of a winter experiment in the South China Sea illustrate that when the water depth is 1 km and distance is 5–8 km,the echo of target is 40 m underwater,there are three highlight clusters with a time interval of about 0.3 s,with the error range of target depth matching estimation being 0–65 m. | ZHANG Fengzhen LI Guijuan HU Wenshuai HU Chen | 2023 | Chinese Journal of Acoustics2023，42，3： | 0 |
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