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| 1 | Effect of Bcl-2 and Bax on survival of side population cells from hepatocellular carcinoma cells显示文摘AIM: To understand the role and significance of side population (SP) cells from hepatocellular carcinoma (HCC) in hepatocarcinogenesis, development, relapse and metastasis, we simulated the denutrition conditions that cancer cells experience in clinical therapy, observed the different anti-apoptosis ability of SP cells and non-SP cells under such conditions, and established the possible effects of P53, Bcl-2 and Bax on survival of SP cells. METHODS: We used ? ow cytometry to analyze and sort the SP and non-SP cells in established HCC lines MHCC97 and hHCC. We evaluated cell proliferation by methyl thiazolyl tetrazolium (MTT) assay and investigated the expression of p53, bcl-2 and bax genes during denutrition, by RT-PCR and immuno? uorescence staining. RESULTS: The percentage of SP cells in the two established HCC lines was 0.25% and 0.5%, respectively. SP cells had greater anti-apoptosis and proliferation ability than non-SP cells. Expression of Bcl-2 and Bax in SP and non-SP cells differed during denutrition. The former was up-regulated in SP cells, and the latter was up-regulated in non-SP cells. CONCLUSION: It may be that different upstream molecules acted and led to different expression levels of Bcl-2 and Bax in these two cell lines. There was a direct relationship between up-regulation of Bcl-2 and down-regulation of Bax and higher anti-apoptosis ability in SP cells. It may be that the existence and activity of SP cells are partly responsible for some of the clinical phenomena which are seen in HCC, such as relapse or metastasis. Further research on SP cells may have potential applications in the f ield of anticancer therapy. | Jing Fan Ren Li Rui Zhang Hai-Liang Liu Ning Zhang Fu-Qing Zhang Ke-Feng Dou | 2007 | World Journal of Gastroenterology2007，13，45： | 27 |
| 2 | Isolation and Identification of Cancer Stem-Like Cells from Murine Melanoma Cell Lines显示文摘In current study,cancer stem-like cells in the murine melanoma B16F10 cells were investigated.CD phenotypes of the B16F10 cells were analyzed by flow cytometry,and the specific CD phenotype cells from the B16F10 cells were isolated by MACS.Then we used colony formation assay in soft agar media,the cell growth assay in serum-free culture media as well as the tumorigenicity investigation of the specific CD phenotype cells in C57BL/6 mice, respectively,to identify cancer stem-like cells in the B16F10 cells.The results showed that the B16F10 cells could form spherical clones in serum-free culture media,and the rate of clonegenesis of CD133 + ,CD44 + and CD44 + CD133 + cells was higher than that of CD133 - ,CD44 - and CD44 + CD133 - cells in soft agar media,respectively. The tumorigenic potential of CD133 + ,CD44 + ,CD44 + CD133 + cells and CD44 + CD133 + CD24 + cells was stronger than that of CD133 - ,CD44 - ,CD44 + CD133 - cells and CD44 + CD133 + CD24 - cells in mice,respectively.In conclusion,the CD44 + CD133 + CD24 + cells have some biological properties of cancer stem-like cells or are highly similar to the characteristics of cancer stem cells(CSC).These results provide an important method for identifying cancer stem-like cells in B16F10 cells and for further cancer target therapy. | Jun Dou Meng Pan Ping Wen Yating Li Quan Tang Lili Chu Fengshu Zhao Chuilian Jiang Weihua Hu Kai Hu Ning Gu | 2007 | Cellular & Molecular Immunology2007，4，6： | 25 |
| 3 | Piglets cloned from induced pluripotent stem cells显示文摘 | Nana Fan Jijun Chen Zhouchun Shang Hongwei Dou Guangzhen Ji Qingjian Zou Lu Wu Lixiazi He Fang Wang Kai Liu Na Liu Jianyong Han Qi Zhou Dengke Pan Dongshan Yang Bentian Zhao Zhen Ouyang Zhaoming Liu Yu Zhao Lin Lin Chongming Zhong Quanlei Wang Shouqi Wang Ying Xu Jing Luan Yu Liang Zhenzhen Yang Jing Li Chunxia Lu Gabor Vajta Ziyi Li Hongsheng Ouyang Huayan Wang Yong Wang Yang Yang Zhonghua Liu Hong Wei Zhidong Luan Miguel A Esteban Hongkui Deng Huanming Yang Duanqing Pei Ning Li Gang Pei Lin Liu Yutao Du Lei Xiao Liangxue Lai | 2013 | Cell Research2013，23，1： | 12 |
| 4 | Treating chronic hepatitis B virus:Chinese physicians' awareness of the 2010 guidelines显示文摘AIM: To investigate Chinese physicians' awareness of the 2010 guidelines on the treatment of chronic hepatitis B virus(HBV) infection.METHODS: This was a quantitative survey that investigated the characteristics and practices of physicians who were treating patients with hepatitis B, the profile of their patients and physician practices regarding the diagnosis and treatment of HBV at the time of the survey. Participants were randomly selected from available databases of Chinese physicians and requested to complete either an online or paper-based survey. Data from the survey responses were analysed. For data validation and interpretation, qualitative in-depth interviews were conducted with 39 of the respondents.RESULTS: Five-hundred completed surveys, from 663 physicians were available for analysis. A mean of 175 chronic hepatitis B(CHB) patients was seen by each physician every month, of whom 85(49%) were treated in line with therapeutic indications stated in the 2010 guidelines. A total of 444(89%) physicians often(> 60% of the time) adhered to the guidelines. Most physicians used antiviral medications as recommended. For patients with compensated and decompensated cirrhosis, 342(68%) and 336(67%) of physicians, respectively, often followed the recommendation to use potent nucleos(t)ide analogues with a high genetic barrier to resistance, using the appropriate treatment more than 60% of the time. Physicians from infectious disease or liver disease departments were better informed than those from gastrointestinal or other departments.CONCLUSION: The majority of Chinese physicians often adhere to Chinese 2010 CHB guidelines and are well-informed about the use of antiviral medications for hepatitis B. | Lai Wei Ji-Dong Jia Xin-Hua Weng Xiao-Guang Dou Jia-Ji Jiang Hong Tang Qin Ning Qing-Qing Dai Run-Qin Li Jie Liu | 2016 | World Journal of Hepatology2016，8，18： | 4 |
| 5 | Geothermal energy exploitation from depleted high-temperature gas reservoirs by recycling CO_(2): The superiority and existing problems显示文摘CO_(2) can be used as an alternative injectant to exploit geothermal energy from depleted high-temperature gas reservoirs due to its high mobility and unique thermal properties.However,there has been a lack of systematic analysis on the heat mining mechanism and performance of CO_(2),as well as the problems that may occur during geothermal energy exploitation at specific gas reservoir conditions.In this paper,a base numerical simulation model of a typical depleted high-temperature gas reservoir was established to simulate the geothermal energy exploitation processes via recycling CO_(2) and water,with a view to investigate whether and/or at which conditions CO_(2) is more suitable than water for geothermal energy exploitation.The problems that may occur during the CO_(2)-based geothermal energy exploitation were also analyzed along with proposed feasible solutions.The results indicate that,for a depleted low-permeability gas reservoir with dimensions of 1000 m×500 m×50 m and temperature of 150℃ using a single injection-production well group for 40 years of operation,the heat mining rate of CO_(2) can be up to 3.8 MW at a circulation flow rate of 18 kg s^(-1)due to its high mobility along with the flow path in the gas reservoir,while the heat mining rate of water is only about 2 MW due to limitations on the injectivity and mobility.The reservoir physical property and injection-production scheme have some effects on the heat mining rate,but CO_(2)always has better performance than water at most reservoir and operation conditions,even under a high water saturation.The main problems for CO_(2) circulation are wellbore corrosion and salt precipitation that can occur when the reservoir has high water saturation and high salinity,in which serious salt precipitation can reduce formation permeability and result in a decline of CO_(2) heat mining rate (e.g.up to 24%reduction).It is proposed to apply a low-salinity water slug before CO_(2)injection to reduce the damage caused by salt precipitation.For high-permeability gas reservoirs with high water saturation and high salinity,the superiority of CO_(2) as a heat transmission fluid becomes obscure and water injection is recommended. | Guodong Cui Shaoran Ren Bin Dou Fulong Ning | 2021 | Geoscience Frontiers2021，12，6： | 3 |
| 6 | Feasibility and safety of robotic PCI in China: first in man experience in Asia显示文摘Objectives To evaluate the feasibility and safety of a second generation robotic percutaneous coronary intervention(R-PCI)system in China.Background Robotic PCI has been shown to be an effective method for conducting coronary interventions.It has further benefits of more accurate lesion measurement,improved stent deployment,reduced incidence of geographic miss and reduction of operator radiation exposure.Methods This single center evaluation enrolled 10 consecutive patients who had been selected for PCI.Clinical success was defined as residual stenosis<30%and no in-hospital major adverse cardiovascular events.Learning curve effect was assessed by comparing efficiency metrics of early vs.later cases.Results Eleven lesions were treated all successfully without manual interruption or MACE events.Most lesions(63%)were ACC/AHA class B2 and C.Mean procedure time was 57.7±26.4 min,however two procedures were part of live demonstrations.Excluding the two live cases,the mean procedure time was 51.8±23.7 min.Procedural efficiency tended to improve from early cases to later cases based on PCI time(48.3±32.9 vs.25.5±13.0 min,P=0.27),fluoroscopy time(20.3±8.2 vs.12.5±4.6 min,P=0.16),contrast volume(145.0±28.9 vs.102.5±17.1 mL,P=0.05)and Air Kerma dose(1932±978 vs.1007±70 mGy,P=0.31).Conclusions Second generation robotic PCI was safe,effective and there were trends toward improvements in procedural efficiency during this early experience in China. | Ke-Fei DOU Chen-Xi SONG Chao-Wei MU Wei-Xian YANG Cheng-Gang ZHU Lei FENG Jue CHEN Lei SONG Yu NING Bo XU | 2019 | Journal of Geriatric Cardiology2019，16，5： | 2 |
| 7 | Excellent strength-ductility synergy properties of gradient nano-grained structural CrCoNi medium-entropy alloy显示文摘Tailoring heterogeneities could bring out excellent strength-ductility synergy properties.A gradient nanograined(GNG)structure,i.e.grain size range from nanometer(~50 nm)at topest surface layer to micrometer(~1.3μm)at center layer,was successfully introduced into CrCoNi medium-entropy alloy(MEA)by means of high energy shot peening in this work.Experimental results demonstrated that this GNG CrCoNi MEA shows excellent strength and ductility combination properties,exhibiting high yield strength and ultimate tensile strength of~1215 MPa and~1524 MPa,respectively,while remaining a good ductility of~23.0%.The extraordinary hetero-deformation induced(HDI)hardening origins from heterogeneous structure,i.e.GNG structure,which contributes to the majority strength enhancement.Dynamical reinforced heterogeneous structure during tension process results in the enhanced HDI hardening effect,which facilitates excellent ductility and strain hardening capacity at high-level strength.Our work provide not only a feasible and effective way to strengthen the CrCoNi MEA,and other low stacking faults energy(SFE)materials,but also an useful insight to understanding HDI hardening in heterogeneous structure. | Wenjie Lu Xian Luo Dou Ning Miao Wang Chao Yang Miaoquan Li Yanqing Yang Pengtao Li Bin Huang | 2022 | Journal of Materials Science & Technology2022，，17： | 2 |
| 8 | Identifying tumor stem-like cells in mouse melanoma cell lines by analyzing the characteristics of side population cells显示文摘 | Jun Dou Ping Wen Weihua Hu Yating Li Yun Wu Chunsheng Liu Fengshu Zhao Kai Hu Jing Wang Chuilian Jiang Xiangfeng He Ning Gu | 2009 | Cell Biology International2009，，8： | 1 |
| 9 | A novel therapeutic system for ma- lignant glioma: nanoformalatlon, pharmacokinetic, and antican- cer properties of cell-nano-drug delivery 显示文摘 | TAO Y NING M DOU H | 2013 | Nanomedicine-Uk2013，9，2： | 1 |
| 10 | Transsphenoidal surgery in a patient with acromegaly and McCune-Albright syndrome:application of neuronavigation显示文摘 | Dou Wan-chen Lin Ning Wang Ren-zhi | 2008 | J Neurosurg2008，108，1： | 1 |
| 11 | Research Progress of Lithium Plating on Graphite Anode in Lithium-Ion Batteries显示文摘Lithium plating on graphite anode is triggered by harsh conditions of fast charge and low temperature,which sig-nificantly accelerates SOH(state of health)degradation and may cause safety issues of lithium ion batteries(LIBs).This paper has reviewed recent research progress of lithium plating on graphite anode.Firstly,we summarize the forming mechanisms of Li plating with(corresponding influence factors,the detect-ing methods and hazard of Li plating.Then,approaches to suppress Li plating are discussed,including anode surface modification,electrolyte composition optimization and development of optimal charge.strategies.Finally,we con-clude and propose the remaining challenges and prospects in terms of mechanism research,detecting approaches,and suppressing methods of Li plating.This review highlights the development of Li plating research and plays a guiding rule of further study on Li plating in LIBs. | Daozhong Hu Lai Chen Jun Tian Yuefeng Su Ning Li Gang Chen Yulu Hu Yueshan Dou Shi Chen Feng Wu | 2021 | Chinese Journal of Chemistry2021，39，1： | 1 |
| 12 | Separation of pro- teins on surface-modified poly (dimethylsiloxane) microfluidic devices 显示文摘 | Dou Ynehua Bao Ning Xu Jingjuan | 2004 | Eleetrophoresis2004，25，17： | 1 |
| 13 | A dynamically modified microfiuidic poly(dimethylsiloxanc) chip with elec- trochemical detection for biological analysis显示文摘 | Dou Yuehua Bao Ning Xu Jingjuan | 2002 | Electrophore- sis2002，23，20： | 1 |
| 14 | Expression of selenoprote P in multiple carcinoma tissues with tissue microarray显示文摘 | WANG Qing LV Ning DOU Ke-feng | 2005 | Chin J Cancer Prey Treat2005，12，10： | 1 |
| 15 | HBeAg-negative Patients with Chronic Hepatitis B Virus Infection and Normal Alanine Aminotransferase:Wait or Treat?显示文摘Alanine aminotransferase(ALT)is a common clinical indicator of liver inflammation.The current Chinese guidelines for the management of chronic hepatitis B(CHB)recommend antiviral treatment for patients with detectable hepatitis B virus(HBV)DNA and persistent ALT levels(ALTs)exceeding the upper limit of normal.However,it has been recently reported that patients with chronic HBV infection,especially HBeAg-negative patients with persistently normal ALTs,may have liver biopsy findings of significant inflammation and fibrosis.For HBeAg-negative patients with chronic HBV infection and normal ALTs,many controversial questions have been asked.To treat or not?When to initiate the treatment?Which drug is appropriate?In this review,we summarize the available data on the management of HBeAg-negative patients with chronic HBV infection and normal ALTs with the aim of improving the current clinical management. | Qiuju Sheng Ning Wang Chong Zhang Yaoxin Fan Yanwei Li Chao Han Ziyi Wang Shuqi Wei Xiaoguang Dou Yang Ding | 2022 | Journal of Clinical and Translational Hepatology2022，10，5： | 1 |
| 16 | Randomized, three‐arm study to optimize lamivudine efficacy in hepatitis B e antigen‐positive chronic hepatitis B patients显示文摘 | Xieer Liang Jun Cheng Yongtao Sun Xinyue Chen Tong Li Hao Wang Jianning Jiang Xiaoping Chen Hui Long Hong Tang Yanyan Yu Jifang Sheng Shijun Chen Junqi Niu Hong Ren Junping Shi Xiaoguang Dou Mobin Wan Jiaji Jiang Qing Xie Guangfeng Shi Qin Ning Chengwei C | 2015 | J Gastroenterol Hepatol2015，，4： | 1 |
| 17 | Paclitaxel-Fe<sub>3</sub>O<sub>4</sub> nanoparticles inhibit growth of CD138<sup>–</sup> CD34<sup>– </sup>tumor stem-like cells in multiple myeloma-bearing mice显示文摘 | Jun Dou Yunxia Zhang Hongyi Zhang Fei Xiong Junsong Chen Dengyu Chen Jing Wang Cuiping Yang Ning Gu | 2013 | International Journal of Nanomedicine (default)2013，，： | 1 |
| 18 | Characterization of fruit development and kernel component variation in candlenut (Aleurite moluccana)显示文摘 | Zige Liu Yan Zhang Yu Ning Weiyan Xuan Lei Wang Dou Feng | 2016 | Oil Crop Science2016，1，3： | 1 |
| 19 | Filaggrin Gene Mutation c.3321delA is Associated with Dry Phenotypes of Atopic Dermatitis in the Chinese Han Population显示文摘INTRODUCTION Atopic dermatitis (AD) is a common chronic inflammatory skin disorder that is characterized by dry skin and disturbed skin barrier functions.Mutations in the filaggrin (FLG) gene,the gene coding profilaggrin/filaggrin,have a great impact on the epidermal barrier function and are an important predisposing factor for AD.However,in both Europeans and Asians,the presence of FLG mutations has been reported to be population-specific.FLG mutation c.3321delA is an Asian-specific mutation and is the most common mutation in the Chinese population,accounting for up to 15% of patients with AD[1] In a previous genome-wide association study of AD,Meng etal.[2] identified FLG variant rs3126085,which was correlated with c.3321delA.Several studies have shown that the c.3321 delA mutation was associated with various AD-associated phenotypes,most of which are related to dry skin phenotypes,including xerosis,ichthyosis vulgaris (Ⅳ),and palmar hyperlinearity.[2] In this study,we used high-resolution melting analysis (HRMA) with unlabeled probe for the detection ofc.3321 delA mutation in Chinese AD patients and investigated the genotype-phenotype correlation between c.3321 delA and atopic characteristics. | Wei-Long Zhong Xia Wu BO YU Jie Zhang Wei Zhang Ning Xu Jing Zhou Jie-Cheng Zheng Xiao-Fan Chen Xia Dou | 2016 | Chinese Medical Journal2016，，12： | 1 |
| 20 | Associations between high levels of Notch1 expression and high invasion and poor overall survival in hepatocellular carcinoma显示文摘 | Liang Zhou Ning Zhang Qing-jun Li Wei Sun Yong Zhang De-sheng Wang Ke-feng Dou | 2013 | Tumor Biology2013，，1： | 1 |
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