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| 1 | PEITC triggers multiple forms of cell death by GSH-iron-ROS regulation in K7M2 murine osteosarcoma cells显示文摘Phenethyl isothiocyanate(PEITC)is an isothiocyanate that largely exists in cruciferous vegetables and exhibits chemopreventive and chemotherapeutic potential against various cancers.However,it is little known about the molecular mechanisms of its antitumor action against osteosarcoma,which is the second highest cause of cancer-related death in children and adolescents.In this study,we investigated the effects of PEITC on K7M2murine osteosarcoma both in vitro and in vivo.We found that treatment with PEITC dose-dependently inhibited the viability of K7M2 murine osteosarcoma cells with an lCso value of 33.49μM at 24h.PEITC(1,15,30μM)dose-dependently inhibited the cell proliferation,caused G/M cell cycle arrest,depleted glutathione(GSH)generated reactive oxygen species(ROS),altered iron metabolism,and triggered multiple forms of cell death,namely ferroptosis apoptosis,and autophagy in K7M2 cells.We further revealed that PEITC treatment activated MAPK signaling pathway,and ROS generation was a major cause ofPEITC-induced cell death.In a syngeneic orthotopic osteosarcoma mouse model,administration of PEITC(30,60 mg/kg every day,ig,for 24 days)significantly inhibited the tumor growth,but higher dose of PEITC(90 mg/kg every day)compromised its ant-osteosarcoma effect.Histological examination showed that multiple cell death processes were initiated,iron metabolism was altered and MAPK signaling pathway was activated in the tumor tissues.In conclusion,we demonstrate that PEITC induces ferroptosis,autophagy,and apoptosis in K7M2 osteosarcoma cell by activating the ROS-elated MAPK signaling pathway.PEITC has promising anti-osteosarcoma activity.This study sheds light on,the redox signaling-based chemotherapeutics for cancers. | Huan-huan Lv Chen-xiao Zhen Jun-yu Liu Peng Shang | 2020 | Acta Pharmacologica Sinica2020，41，8： | 12 |
| 2 | Design and development of an oral remdesivir derivative W116 against SARS-CoV-2显示文摘Dear Editor,Since the declaration of COVID-19 as a global pandemic on March 11,2020,this pandemic has been circulating for 17 months throughout the world,leading to more than 200 million infections and nearly 4.4 million deaths as of August 15,2021.The pathogen of COVID-19 is a novel coronavirus named SARS-CoV-2,which shares-79%genome sequence identity with SARS-CoV.1 At the early stage of the COVID-19 outbreak,5ARS-CoV-2 caused great panic in the hardest-hit areas due to its high transmissibility and pathogenicity.To fight the COVID-19 crisis,drug repurposing was immediately pursued in order to find potential therapeutics. | Yuanchao Xie Wanchao Yin Yumin Zhang Weijuan Shang Zhen Wang Xiaodong Luan Guanghui Tian Haji A.Aisa Yechun Xu Gengfu Xiao Jia Li Hualiang Jiang Shuyang Zhanq Leike Zhang H.Eric Xu Jingshan Shen | 2021 | Cell Research2021，31，11： | 12 |
| 3 | Piglets cloned from induced pluripotent stem cells显示文摘 | Nana Fan Jijun Chen Zhouchun Shang Hongwei Dou Guangzhen Ji Qingjian Zou Lu Wu Lixiazi He Fang Wang Kai Liu Na Liu Jianyong Han Qi Zhou Dengke Pan Dongshan Yang Bentian Zhao Zhen Ouyang Zhaoming Liu Yu Zhao Lin Lin Chongming Zhong Quanlei Wang Shouqi Wang Ying Xu Jing Luan Yu Liang Zhenzhen Yang Jing Li Chunxia Lu Gabor Vajta Ziyi Li Hongsheng Ouyang Huayan Wang Yong Wang Yang Yang Zhonghua Liu Hong Wei Zhidong Luan Miguel A Esteban Hongkui Deng Huanming Yang Duanqing Pei Ning Li Gang Pei Lin Liu Yutao Du Lei Xiao Liangxue Lai | 2013 | Cell Research2013，23，1： | 12 |
| 4 | Comprehensive analysis of NAC transcription factors in diploid Gossypium: sequence conservation and expression analysis uncover their roles during fiber development显示文摘Determining how function evolves following gene duplication is necessary for understanding gene expansion.Transcription factors(TFs)are a class of proteins that regulate gene expression by binding to specific cis-acting elements in the promoters of target genes,subsequently activating or repressing their transcription.In the present study,we systematically examined the functional diversification of the NAC transcription factor(NAC-TFs)family by analyzing their chromosomal location,structure,phylogeny,and expression pattern in Gossypium raimondii(Gr)and G.arboreum(Ga).The 145 and 141 NAC genes identified in the Gr and Ga genomes,respectively,were annotated and divided into 18 subfamilies,which showed distinct divergence in gene structure and expression patterns during fiber development.In addition,when the functional parameters were examined,clear divergence was observed within tandem clusters,which suggested that subfunctionalization had occurred among duplicate genes.The expression patterns of homologous gene pairs also changed,suggestive of the diversification of gene function during the evolution of diploid cotton.These findings provide insights into the mechanisms underlying the functional differentiation of duplicated NAC-TFs genes in two diploid cotton species. | Haihong Shang Zhongna Wang Changsong Zou Zhen Zhang Weijie Li Junwen Li Yuzhen Shi Wankui Gong Tingting Chen Aiying Liu Juwu Gong Qun Ge Youlu Yuan | 2016 | Science China(Life Sciences)2016，59，2： | 12 |
| 5 | Antitumor activity of anti-type IV collagenase monoclonal antibody and its lidamycin conjugate against colon carcinoma显示文摘瞄准:包括 MMP-2 和 -9 的类型 IV 胶原酶在癌症房间侵略和转移起一个重要作用并且是为指导 mAb 的治疗的一个吸引人的目标。mAb 3G11 的免疫反应,在人对类型 IV 胶原酶指导的 mAb 渲染表面的癌,被学习由免疫组织化学(IHC ) 染色。mAb 3G11 被结合到反肿瘤抗菌素 lidamycin (LDM ) 。对冒号癌的 3G11-LDM conjugate 的反肿瘤活动在老鼠被调查。方法:ELISA,明胶 zymography,和西方的污点试金被用于 mAb 3G11 的生物描述。有人的 mAb 3G11 的免疫反应渲染表面的癌被染色的 IHC 检测。到人的结肠癌 HT-29 房间的 LDM 和 3G11-LDM conjugate 的细胞毒性被 clonogenic 试金和 MTT 试金检验。conjugate 3G11-LDM 的治疗学的效果与在老鼠的冒号癌 26 被评估。结果:是出现在 ELISA, mAb 3G11 与类型 IV 胶原酶明确地反应了,当 3G11-LDM conjugate 也明确地认出了它的各自的抗原时。在 IHC 试金, mAb 3G11 在颜色的大多数情况中显示出积极免疫反应表面的癌,和在邻近的非恶意的纸巾的否定免疫反应。由明胶 zymography,在类型 IV 胶原酶的分泌物活动的 mAb 3G11 的抑制效果被证明。以在 MTT 试金的 IC50 价值,到人的结肠癌 HT-29 房间的 LDM 的细胞毒性更是 10,000 褶层比丝裂霉素C(MMC ) 和 adriamycin (ADM ) 的有势力。3G11-LDM conjugate 也极其与 5.6 x 10 的 IC50 价值显示了有势力细胞毒性到人的冒号癌 HT-29 房间(-19) mol/L。在 0.05 和 0.1 mg/kg 的剂量的 3G11-LDM conjugate 分别地在 70.3 和 81.2% 禁止了在老鼠的结肠癌 26 的生长。结论:mAb 3G11 是与有 LDM 的表面的癌和它的 conjugate 是的人的颜色反应的免疫对在老鼠的冒号癌高度有效。 | Liang Li Yun-Hong Huang Yi Li Feng-Qiang Wang Bo-Yang Shang Yong-Su Zhen | 2005 | World Journal of Gastroenterology2005，11，29： | 9 |
| 6 | Cationic content effects of biodegradable amphoteric chitosan-based flocculants on the flocculation properties显示文摘A series of biodegradable amphoteric chitosan-based flocculants(3-chloro-2-hydroxypropyl trimethyl ammonium chloride(CTA) modified carboxymethyl chitosan,denoted as CMC-CTA) with different substitution degrees of CTA were prepared successfully.The content of carboxymethyl groups in each CMC-CTA sample was kept almost constant.The solubility of the various flocculants showed that,higher cationic content of flocculants caused a better solubility.The flocculation experiments using kaolin suspension as synthetic water at the laboratory scale indicated that the substitution degree of CTA was one of the key factors for the flocculation properties.With the increase of cationic content,the flocculants were demonstrated better flocculation performance and lower dosage requirement.Flocculation kinetics model of particles collisions combining zeta potential and turbidity measurements was employed to investigate the effects of the cationic content of the flocculants on the flocculation properties from the viewpoint of flocculation mechanism in detail.Furthermore,flocculation performance using raw water from Zhenjiang part of Yangtze River at the pilot scale showed the similar effects to those at the laboratory scale. | Zhen Yang Yabo Shang Xin Huang Yichun Chen Yaobo Lu Aimin Chen Yuxiang Jiang Wei Gu Xiaozhi Qian Hu Yang Rongshi Cheng | 2012 | Journal of Environmental Sciences2012，24，8： | 8 |
| 7 | Antitumor effects of the molecule-downsized immunocon-jugate composed of lidamycin and Fab' fragment of monoclonal antibody directed against type IV collagenase显示文摘Type IV collagenase plays an important role in tumor invasion and metastasis through cleaving type IV collagen in the basement membrane and extracellular matrix. In this study a molecule-downsized immunoconjugate (Fab′-LDM) was constructed by linking lidamycin (LDM), a highly potent antitumor antibiotic, to the Fab′ fragment of a monoclonal antibody directed against type IV collagenase and its antitumor effect was investigated. As assayed in 10% SDS-PAGE gel, the molecular weight of Fab′-LDM conjugate was 65 kD with a 1:1 molecular ratio of Fab′ and LDM. The Fab′-LDM conjugate maintained most part of the immunoreactivity of Fab′ fragment to both type IV collagense and mouse hepatoma 22 cells by ELISA. By MTT assay, Fab′-LDM conjugate showed more potent cytotoxicity to hepatoma 22 cells than that of LDM. Administered intravenously, Fab′-LDM conjugate proved to be more effective against the growth of subcutaneously transplanted hepatoma 22 in mice than free LDM in two experiment settings. In Experiment I, the drugs were given intravenously on day 1 and day 8. Fab′-LDM at the doses of 0.025 mg/kg, 0.05 mg/kg and 0.1 mg/kg inhibited tumor growth by 76.7%, 93.3% and 94.8%, while free LDM at 0.05 mg/kg inhibited tumor growth by 76.1%, respectively. In experiment II, the drugs were given intravenously on day 4 and day 11, Fab′-LDM at the doses of 0.025 mg/kg and 0.05 mg/kg inhibited tumor growth by 74.2%, 80.9%, while free LDM at 0.05 mg/kg inhibited tumor growth by 60.5%, respectively. In terms of survival time, Fab′-LDM was more effective than free LDM. The results suggest that the molecule-downsized immunoconjugate directed against type IV collagenase is of high efficacy in experimental cancer therapy. | WANG Fengqiang SHANG Boyang ZHEN Yongsu | 2004 | Science China(Life Sciences)2004，47，1： | 7 |
| 8 | Oil spill cleanup from sea water by carbon nanotube sponges显示文摘在海的油喷洒引起了许多世界范围的严重环境问题。在这研究,碳 nanotube (CNT ) 海绵在海水上习惯于清除油 slicks。这个方法与二传统的代表性的吸着剂相比,包括聚丙烯纤维织物和羊毛毡。CNT 海绵比另外的二吸着剂有一个更大的油吸着能力。最大的油吸着能力( Q <潜水艇class=“ a-plus-plus ”> CNT 海绵的 m )是 92.30 g/g ,它比 Q 大 12~13.5 倍<潜水艇class=“ a-plus-plus ”>另外的二吸着剂的 m ( Q <潜水艇class=“ a-plus-plus ”>聚丙烯纤维织物和羊毛毡的 m 是 7.45 和 6.74 g/g ,分别地)。另外 CNT 海绵不同于另外的二吸着剂,是 superhydrophobic 并且没在油喷洒清除期间吸附任何水。CNT 海绵是潜在地为从海水清理油喷洒很有用。 | Ke ZHU Yuan-Yuan SHANG Peng-Zhan SUN Zhen LI Xin-Ming LI Jin-Quan WEI Kun-Lin WANG De-Hai WU An-Yuan CAO Hong-Wei ZHU | 2013 | Frontiers of Materials Science2013，7，2： | 7 |
| 9 | Prevalence of late-onset hypogonadism among middle-aged and elderly males in China:results from a national survey显示文摘This study aimed to propose an operational definition of late-onset hypogonadism(LOH)that incorporates both clinical symptoms and serum testosterone measurements to evaluate the prevalence of LOH in aging males in China.A population-based sample of 6296 men aged 40 years-79 years old was enrolled from six representative provinces in China.Serum total testosterone(TT),sex hormone-binding globulin(SHBG),and luteinizing hormone(LH)were measured and free testosterone(cFT)was calculated.The Aging Males’Symptoms(AMS)scale was used to evaluate the LOH symptoms.Finally,5078 men were included in this analysis.The TT levels did not decrease with age(P=0.59),and had no relationship with AMS symptoms(P=0.87 for AMS total score,P=0.74 for≥3 sexual symptoms).The cFT levels decreased significantly with age(P<0.01)and showed a negative association with the presence of≥3 sexual symptoms(P=0.03).The overall estimated prevalence of LOH was 7.8%(395/5078)if a cFT level<210 pmol l−1 combined with the presence of≥3 sexual symptoms was used as the criterion of LOH.Among them,26.1%(103/395)and 73.9%(292/395)had primary and secondary hypogonadism,respectively.After adjustment for confounding factors,primary and secondary hypogonadism was positively related to age and comorbidities.Body mass index was an independent risk factor for secondary hypogonadism.The results suggest that the AMS total score is not an appropriate indicator for decreased testosterone,and that the cFT level is more reliable than TT for LOH diagnosis.Secondary hypogonadism is the most common form of LOH. | Yi-Jun Liu Xu-Bo Shen Na Yu Xue-Jun Shang Yi-Qun Gu Lian-Dong Zuo Cheng-Liang Xiong Zhen Ye Yuan-Zhong Zhou | 2021 | Asian Journal of Andrology2021，23，2： | 6 |
| 10 | Integrated genomic analysis identifies deregulated JAK/STAT-MYC-biosynthesis axis in aggressive NK-cell leukemia显示文摘好攻击的 NK 房间白血病(ANKL ) 是在从亚洲的人之中是更流行的 NK 房间瘤的一种稀罕形式并且中央并且南美洲。病人们通常在天以内死到月,甚至在收到迅速的治疗学的管理以后。这里,我们由集成整个染色体执行了 ANKL 的第一全面研究, transcriptome 并且定序指向,象功能的试金一样的 cytokine 数组。在 JAK-STAT 小径的变化在 48% 被识别(14/29 ) ANKL 病人,当细胞外的 STAT3 激发器 IL10 被 56 褶层的一般水准提高时(P < 0.0001 ) 在检查的所有病人的血浆。另外的经常变异的基因包括的 TP53 (34%) , TET2 (28%) , CREBBP (21%) 和 MLL2 (21%) 。耐心的 NK 白血病房间在多重新陈代谢的小径显示出 STAT3 phosphorylation, MYC 表示和 transcriptional 活动的突出的激活。机能上地, STAT3 激活和 MYC 表示为 ANKL 房间的增长和幸存是批评的。发信号的 STAT 调整了发信号的 MYC 抄写节目,和两 STAT, MYC 抄写被要求维持核苷酸合成和 glycolysis 的激活。一起, JAK-STAT 小径在 ANKL 为 genomic 改变和 IL10 刺激代表一个主要目标。这最新发现的 JAK/STAT-MYC-biosynthesis 轴可以在对待白血病的这种子类型为新奇治疗学的策略的发展提供机会。 | Liang Huang Dan Liu Na Wang Shaoping Ling Yuting Tang Jun Wu Lingtong Hao Hui Luo Xuelian Hu Lingshuang Sheng Lijun Zhu Di Wang Yi Luo Zhen Shang Min Xiao Xia Mao Kuangguo Zhou Lihua Cao Lili Dong Xinchang Zheng Pinpin Sui Jianlin He Shanlan Mo Jin Yan Qilin Ao Lugui Qiu Hongsheng Zhou Qifa Liu Hongyu Zhang Jianyong Li Jie Jin Li Fu Weili Zhao Jieping Chen Xin Du Guoliang Qing Hudan Liu Xin Liu Gang Huang Ding Ma Jianfeng Zhou Qian-fei Wang | 2018 | Cell Research2018，28，2： | 5 |
| 11 | Water-induced ultralong room temperature phosphorescence by constructing hydrogen-bonded networks显示文摘Room temperature phosphorescence(RTP)materials show potential applications in information security and optoelectronic devices,but it is still a challenge to achieve RTP in organic materials under water ambient due to the unstable property of triplet states.Herein,water-induced RTP has been demonstrated in the organic microrod(OMR).Noting that the RTP intensity of the as-prepared OMR is greatly enhanced when water is introduced,and the reason for the enhancement can be attributed to the formation of hydrogen-bonded networks inside the OMR.The hydrogen-bonded networks can confine the molecular motion effectively,leading to the stability of triplet states;thus the lifetime of the OMR can reach 1.64 s after introducing water.By virtue of the long lifetime of the OMR in the presence of water,multilevel data encryption based on the OMR has been demonstrated. | Ya-Chuan Liang Yuan Shang Kai-Kai Liu Zhen Liu Wen-Jie Wu Qian Liu Qi Zhao Xue-Ying Wu Lin Dong Chong-Xin Shan | 2020 | Nano Research2020，13，3： | 5 |
| 12 | The Weather Temperature and Air Pollution Interaction and Its Effect on Hospital Admissions due to Respiratory System Diseases in Western China显示文摘Air pollution has ever become a global major public health problem.Previous studies showed that air pollution is associated with excessive mortality and morbidity of respiratory disease[1-2].The extreme weather temperature can impact human health and the thermal stresses can lead not only to direct deaths and illnesses,but also to aggravation of respiratory disease[3-4]. | WANG Min Zhen ZHENG Shan WANG Shi Gong TAO Yan SHANG Ke Zheng | 2013 | Biomedical and Environmental Sciences2013，26，5： | 5 |
| 13 | Vertical migration through faults and hydrocarbon accumulation patterns in deepwater areas of the Qiongdongnan Basin显示文摘In the Qiongdongnan Basin, faults are well developed. Based on the drilling results, the traps controlled two or more faults are oil-rich. However, when only one fault cut through the sand body, there is no sign for hydrocarbon accumulation in the sandstone. In terms of this phenomenon, the principle of reservoir-forming controlled by fault terrace is proposed, i.e., when the single fault activates, because of the incompressibility of pore water, the resistance of pore and the direction of buoyancy, it is impossible for hydrocarbon to accumulate in sandstone. But when there are two or more faults, one of the faults acts as the spillway so the hydrocarbon could fill in the pore of sandstone through other faults. In total five gas bearing structures and four failure traps are considered, as examples to demonstrate our findings. According to this theory, it is well-advised that south steep slope zone of Baodao-Changchang Depression, south gentle slope zone of Lingshui Depression, north steep slope zone of Lingshui Depression, and north steep slope zone of Baodao Depression are the most favorable step-fault zones, which are the main exploration direction in next stage. | WANG Zhenfeng LIU Zhen CAO Shang SUN Zhipeng ZUO Qianmei WANG Yahui HE Weijun | 2014 | Acta Oceanologica Sinica2014，33，12： | 4 |
| 14 | High-efficiency extraction synthesis for high-purity copper nanowires and their applications in flexible transparent electrodes显示文摘Copper nanowires(Cu NWs)are considered an excellent alternative to indium tin oxide(ITO)in flexible transparency electrodes(FTEs).However,the mixed particles and surface oxidation of Cu NWs degrade the transmittance and conductivity of the electrodes.Therefore,highly purified Cu NWs without oxidation are vital for high-performance FTEs.Herein,a facile and effective purification process is introduced to purify Cu NWs in a water and n-hexane system,which takes advantage of the differences in hydrophilicity between Cu NWs and Cu NPs caused by their different adsorption affinities to octadecylamine(ODA).At the same sheet resistance,the transmittance of the purified Cu NW-based FTEs improved approximately 2%compared to that of non-purified Cu NW-based FTEs.Immersion of the electrode in glacial acetic acid removed the surface organics and oxides.After only 40 s of treatment,the sheet resistance dramatically decreased from 10^5 Ohm/sq to 31 Ohm/sq with a transmittance of 85%.In addition,the Cu NW-based FTE conductors showed excellent flexibility(remaining stable after 1000 bending cycles).The Cu NW-based FTEs were further applied to fabricate a flexible transparent heater.At a voltage of 10 V,the temperature of the heater reached 73℃,demonstrating the potential applications of this material in various fields. | He Zhang Shang Wang Yanhong Tian Jiayue Wen Chunjin Hang Zhen Zheng Yilong Huang Su Ding Chenxi Wang | 2020 | Nano Materials Science2020，2，2： | 4 |
| 15 | From Mind to Products:Towards Social Manufacturing and Service显示文摘After reviewing the development of industrial manufacturing, a novel concept called social manufacturing(SM) and service are proposed as an innovative manufacturing solution for the coming personalized customization era. SM can realize a customer's requirements of 'from mind to products', and fulfill tangible and intangible needs of a prosumer, i.e., producer and consumer at the same time. It represents a manufacturing trend,and is expected to become popular in more and more industries.First, a comparison between mass customization and SM is given out, and the basis and motivation from social network to SM is analyzed. Then, its basic theories and supporting technologies,like Internet of Things(Io T), social networks, cloud computing,3 D printing, and intelligent systems, are introduced and analyzed,and an SM platform prototype is developed. Finally, three transformation modes towards SM and 3 D printing are suggested for different user cases. | Gang Xiong Fei-Yue Wang Timo R. Nyberg Xiuqin Shang Mengchu Zhou Zhen Shen Shuangshuang Li Chao Guo | 2018 | IEEE/CAA Journal of Automatica Sinica2018，5，1： | 3 |
| 16 | First experimental constraints on WIMP couplings in the effective field theory framework from CDEX显示文摘We present weakly interacting massive particles(WIMPs) search results performed using two approaches of effective field theory from the China Dark Matter Experiment(CDEX), based on the data from both CDEX-1B and CDEX-10 stages. In the nonrelativistic effective field theory approach, both time-integrated and annual modulation analyses were used to set new limits for the coupling of WIMP-nucleon effective operators at 90% confidence level(C.L.) and improve over the current bounds in the low mχregion. In the chiral effective field theory approach, data from CDEX-10 were used to set an upper limit on WIMP-pion coupling at 90% C.L. We for the first time extended the limit to the m_(χ)<6 GeV/c^(2) region. | Yi Wang Zhi Zeng Qian Yue LiTao Yang KeJun Kang YuanJing Li Mehment Agartioglu HaiPeng An JianPing Chang JingHan Chen YunHua Chen JianPing Cheng Cheng Yi Chiang WenHan Dai Zhi Deng ChangHao Fang XinPing Geng Hui Gong QiuJu Guo XuYuan Guo HongJian He Li He ShengMing He JinWei Hu TuChen Huang HanXiong Huang HaiTao Jia LiPing Jia Xi Jiang HauBin Li JianMin Li Jin Li MingXuan Li RenMingJie Li Xia Li YuLan Li Bin Liao FongKay Lin ShinTed Lin ShuKui Liu YanDong Liu Yu Liu YuanYuan Liu ZhongZhi Liu Hao Ma YuCai Mao QiYuan Nie JinHua Ning Hui Pan NingChun Qi Jie Ren XiChao Ruan ChangSong Shang Vivek Sharma Ze She Lakhwinder Singh Monoj Kumar Singh TianXi Sun ChangJian Tang WeiYou Tang Yang Tian GuangFu Wang Li Wang Qing Wang Yu Chen Wang YunXiang Wang Zhen Wang Henry Tsz-King Wong ShiYong Wu YuCheng Wu HaoYang Xing Yin Xu Tao Xue YuLu Yan Nan Yi ChunXu Yu HaiJun Yu JianFeng Yue Ming Zeng BingTao Zhang Lei Zhang FengShou Zhang ZhenYu Zhang KangKang Zhao MingGang Zhao JiFang Zhou ZuYing Zhou JingJun Zhu | 2021 | Science China(Physics,Mechanics & Astronomy)2021，64，8： | 3 |
| 17 | Generation and antitumor effects of an engineered and energized fusion protein VL-LDP-AE composed of single-domain antibody and lidamycin显示文摘Type IV collagenase plays a pivotal role in invasion, metastasis and angiogenesis of tumor. Single domain antibodies are attractive as tumor-targeting vehicle because of their much smaller size com-pared with antibody molecules produced by conventional methods. Lidamycin (LDM) is a potent enediyne-containing antitumor antibiotic. In this study an engineered and energized fusion protein VL-LDP-AE composed of lidamycin and VL domain of mAb 3G11 directed against type IV collagenase was prepared using a novel two-step method. First a VL-LDP fusion protein was constructed by DNA recombination. Secondly VL-LDP-AE was obtained by molecular reconstitution. In MTT assay, VL-LDP-AE showed potent cytotoxicity to HT-1080 cells and KB cells with IC50 values of 8.55×10-12 and 1.70×10-11 mol/L, respectively. VL-LDP-AE showed antiangiogenic activity in chick chrorioallantoic membrane (CAM) assay and tube formation assay. In in vivo experiments, VL-LDP-AE was proved to be more effective than free LDM against the growth of subcutaneously transplanted hepatoma 22 in mice. Drugs were given intravenously on day 3 and 10 after tumor transplantation. Compared in terms of maximal tolerated doses, VL-LDP-AE at 0.25 mg/kg suppressed the tumor growth by 89.5%, LDM at 0.05 mg/kg by 69.9%, and mitomycin at 1 mg/kg by 35%. Having a molecular weight of 25.2 kDa, VL-LDP-AE was much smaller than other reported antibody-based drugs. The results suggested that VL-LDP-AE would be a promising candidate for tumor targeting therapy. And the 2-step approach could serve as a new technology platform for making a series of highly potent engineered antibody-based drugs for a variety of cancers. | MIAO QingFang, SHANG BoYang, OUYANG ZhiGang, LIU XiaoYun & ZHEN YongSu Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China | 2007 | Science China(Life Sciences)2007，50，4： | 3 |
| 18 | A New Steroid from Polygonum nodosum Pers显示文摘A new steroid named as Zhonghualiaoine I (1) was isolated from Polygonum nodosum Pers (Chinese name 揨honghualiao? Berberidaceae). Its structure was deduced on the basis of its spectral data. | Shang Zhen ZHENG Tong SHEN 等 | 2003 | Chinese Chemical Letters2003，14，2： | 3 |
| 19 | Low Phase Angle Predicts Poor Survival in Patients with Hepatocellular Carcinoma:A Retrospective Study显示文摘Background The phase angle(PA)is an indicator of nutritional status derived from bioelectrical impedance analysis(BIA).Studies have shown that the PA is one of several factors related to the survival of cancer patients.However,the correlation between the PA and prognosis in patients with hepatocellular carcinoma(HCC)has not been reported.This study aimed to explore the relationship between the PA,nutrition-related indicators,and patient prognosis.Methods The data from 248 HCC patients who underwent BIA between January 2015 and December 2019 at the First Hospital of Jilin University were retrospectively analyzed.Patients were divided into two groups according to the median PA value,then the nutrition-related indicators and overall survival(OS)were compared between the two groups.The correlations between the PA and laboratory test nutrition-related indicators and the results of a body composition analysis were determined using Pearson correlation analysis.Independent predictors of the patient prognosis were identified using univariate and multivariate analyses.Results The median OS in the high and low PA groups were 70 and 54.6 months,respectively(P=0.004).The Pearson correlation analysis revealed that the PA was associated with both laboratory test nutrition-related indicators and the results of a body composition analysis.The multivariable Cox regression analysis identified the PA as an independent risk factor affecting the prognosis of patients with Barcelona Clinic Liver Cancer stage A or B.Conclusion The PA is a good biomarker for the nutritional status.We found that the PA was associated with the prognosis in patients with HCC,with a low PA indicating a poor nutritional status and worse prognosis. | Jin Yu Shi Guang Yang Bo Liu Xiao Shang Guo Zhen Cui Jia Xin Huang Wan Ting Wang Ke Yu Chen Nan Ya Wang | 2022 | Journal of Nutritional Oncology2022，7，2： | 2 |
| 20 | Behavior of apatite in granitic melts derived from partial melting of muscovite in metasedimentary sources显示文摘Fluid-absent and fluid-fluxed melting of muscovite in metasedimentary sources are two types of crustal anatexis to produce the Himalaya Cenozoic leucogranites.Apatite grains separated from melts derived from the two types of parting melting have different geochemical compositions.The leucogranites derived from fluid-fluxed melting have relict apatite grains and magmatic crystallized apatite grains,by contrast,there are only crystallized apatite grains in the leucogranites derived from fluid-absent melting.Moreover,apatite grains crystallized from fluid-fluxed melting of muscovite contain higher Sr,but lower Th and LREE than those from fluid-absent melting of muscovite,which could be controlled by the distribution of partitioning coefficient(D_(Ap/Melt))between apatite and leucogranite.D_(Ap/Melt) in granites derived from fluidabsent melting is higher than those from fluid-fluxed melting.So,not only SiO_(2) and A/CNK,but also types of crustal anatexis are sensitive to trace element partition coefficients for apatite.In addition,due to being not susceptible to alteration,apatite has a high potential to yield information about petrogenetic processes that are invisible at the whole-rock scale and thus is a useful tool as a petrogenetic indicator. | Li-E Gao Ling-sen Zeng Ling-hao Zhao Jia-hao Gao Zhen Shang | 2021 | China Geology2021，4，1： | 2 |
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