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| 1 | Simulating the Effect of Hydrate Dissociation on Wellhead Stability During Oil and Gas Development in Deepwater显示文摘It is well known that methane hydrate has been identified as an alternative resource due to its massive reserves and clean property. However, hydrate dissociation during oil and gas development(OGD) process in deep water can affect the stability of subsea equipment and formation. Currently, there is a serious lack of studies over quantitative assessment on the effects of hydrate dissociation on wellhead stability. In order to solve this problem, ABAQUS finite element software was used to develop a model and to evaluate the behavior of wellhead caused by hydrate dissociation. The factors that affect the wellhead stability include dissociation range, depth of hydrate formation and mechanical properties of dissociated hydrate region. Based on these, series of simulations were carried out to determine the wellhead displacement. The results revealed that, continuous dissociation of hydrate in homogeneous and isotropic formations can causes the non-linear increment in vertical displacement of wellhead. The displacement of wellhead showed good agreement with the settlement of overlying formations under the same conditions. In addition, the shallower and thicker hydrate formation can aggravate the influence of hydrate dissociation on the wellhead stability. Further, it was observed that with the declining elastic modulus and Poisson's ratio, the wellhead displacement increases. Hence, these findings not only confirm the effect of hydrate dissociation on the wellhead stability, but also lend support to the actions, such as cooling the drilling fluid, which can reduce the hydrate dissociation range and further make deepwater operations safer and more efficient. | LI Qingchao CHENG Yuanfang ZHANG Huaiwen YAN Chuanliang LIU Yuwen | 2018 | Journal of Ocean University of China2018，17，1： | 11 |
| 2 | An overview of design for CSNS/RCS and beam transport显示文摘The China Spallation Neutron Source (CSNS) is the first accelerator-based pulsed neutron source in China. Its accelerators are made up of an 80 MeV H- linac, a Rapid Cycling Synchrotron (RCS) and two beam transport lines. RCS accumulates and accelerates protons to the design energy of 1.6 GeV, and extracts high energy beam to strike the target. The overview of RCS is presented, and the key problems of the physics design are discussed. The two beam transport lines, from linac to RCS and from RCS to the target, are also introduced. | WANG Sheng AN YuWen FANG ShouXian HUANG Nan LIU WeiBin LIU YuDong QIN Qing QIU Jing WANG Na XU Gang XU ShouYan YU ChengHui | 2011 | Science China(Physics,Mechanics & Astronomy)2011，54，S2： | 11 |
| 3 | Loss of 5-hydroxymethylcytosine is linked to gene body hypermethylation in kidney cancer显示文摘5-methylcytosine (5mC ) 和它的氧化形式 5-hydroxymethylcytosine (5hmC ) 被建议了涉及 tumorigenesis。因为印射方法的广泛地使用的 5mC 的读出,定序的重亚硫酸盐(BS-seq ) ,是 5mC 的和, 5hmC 层次, 5mC/5hmC 模式和这二修正的关系仍然保持糟糕理解。由介绍真实 5mC (BS-seq 由帮助 Tet 的 BS-seq 改正了, TAB-seq ) 并且 5hmC (TAB-seq ) 在单个核苷酸的分辨率同时铺平,我们这里证明与匹配的正常纸巾相比没有在肾肿瘤的 5mC 的全球损失。相反地, 5hmC 全球性被失去在几乎所有肾肿瘤纸巾。在肿瘤纸巾的 5hmC 水平是为肾癌症的一个独立预示的标记,与与更短的全面幸存联系的 5hmC 的底层。而且,我们证明 5hmC 的损失与匹配的正常纸巾相比在肿瘤被连接到 hypermethylation,特别地在基因身体区域。惊人地,基因身体 hypermethylation 显著地与肿瘤相关的基因的 silencing 被联系。IDH1 的 Downregulation 作为在肾癌症位于 5hmC 损失下面的机制被识别。恢复 5hmC 层次稀释了肿瘤房间的侵略能力并且在一个异种皮移植模型压制了肿瘤生长。一起,我们的结果证明 5hmC 的损失在由改变 DNA methylation 模式的肾癌症是一个预示的标记和一个 oncogenic 事件。 | Ke Chen Jing Zhang Zhongqiang Guo Qin Ma Zhengzheng Xu Yuanyuan Zhou Ziying Xu Zhongwu Li Xiongjun- Ye Xuesong Li Bifeng Yuan Yuwen Ke Chuan He Liqun Zhou Jiang Liu WeiminCi | 2016 | Cell Research2016，26，1： | 10 |
| 4 | Prioritizing natural-selection signals from the deep-sequencing genomic data suggests multi-variant adaptation in Tibetan highlanders显示文摘Human genetic adaptation to high altitudes(>2500 m)has been extensively studied over the last few years,but few functional adaptive genetic variants have been identified,largely owing to the lack of deep-genome sequencing data available to previous studies.Here,w e build a list of putative adaptive variants,including 63 missense,7 loss-of-function;1,298 evolutionarily conserved variants and 509 expression quantitative traits loci.Notably,the top signal of selection is located in TMEM 247,a transmembrane protein-coding gene.The Tibetan version of TMEM 247 harbors one high-frequency(76.3%)missense variant,rsl 16983452(c.248C>T;p.Ala83Val);with the T allele derived from archaic ancestry and carried by>94%of Tibetans but absent or in low frequencies(<3%)in non-Tibetan populations.The rsl 16983452-T is strongly and positively correlated with altitude and significantly associated with reduced hemoglobin concentration(p=5.78×10^-5),red blood cell count(p=5.72×10^-7)and hematocrit(p=2.57×10^-6).In particular,TMEM247-rs 116983452 shows greater effect size and better predicts the phenotypic outcome than any E P A S1 variants in association with adaptive traits in Tibetans.Modeling the interaction between TM£M247-rsl 16983452 and EPAS1 variants indicates weak but statistically significant epistatic effects.Our results support that multiple variants may jointly deliver the fitness of the Tibetans on the plateau,where a complex model is needed to elucidate the adaptive evolution mechanism. | Lian Deng Chao Zhang Kai Yuan Yang Gao Yuwen Pan Xueling Ge Yaoxi He Yuan Yuan Yan Lu Xiaoxi Zhang Hao Chen Haiyi Lou Xiaoji Wang Dongsheng Lu Jiaojiao Liu Lei Tian Qidi Feng Asifullah Khan Yajun Yang Zi-Bing Jin Jian Yang Fan Lu Jia Qu Longli Kang Bing Su Shuhua Xu | 2019 | National Science Review2019，6，6： | 9 |
| 5 | Appraisal of the Quality and Contents of Clinical Practice Guidelines for Hypertension Management in Chinese Medicine: A Systematic Review显示文摘Objective: To evaluate the quality and consistency of recommendations in the clinical practice guidelines(CPGs) for hypertension in Chinese medicine(CM). Methods: CM CPGs were identified from 5 electronic databases and hand searches through related handbooks published from January 1990 to December 2013. Three reviewers independently appraised the CPGs based on the Appraisal of Guidelines for Research and Evaluation(AGREE Ⅱ) instrument, and compared the CPGs' recommendations on CM syndrome pattern classification and treatment. Results: Five CM CPGs for hypertension were included. The quality score of the evidence-based(EB) guideline was higher than those of the consensus-based with no explicit consideration of evidence-based(CB-EB) and the consensus-based(CB) guidelines. Three out of five patterns in the CPGs were recommended by the EB guideline. Tianma Gouteng Formula(天麻钩藤复方) in the EB guideline was recommended mostly for hypertension patients with pattern of ascendant hyperactivity of Gan(Liver)-yang and pattern of yin deficiency with yang hyperactivity in the CPGs. Acupuncture and massage were recommended for Grade Ⅰ and Grade Ⅱ hypertension with severe symptoms weakening the quality of life in the EB guideline. For Grade Ⅰ and Grade Ⅱ hypertension, CM could be used alone, while for Grade Ⅲ hypertension, they should be used in combination with Western medicines. Conclusion: The quality of EB guideline was higher than those of CB and CB-EB CPGs in CM for hypertension and CM should be prescribed alone or combined with Western medicines based on the grade of hypertension. | YUWEN Ya HAN Xue-jie WENG Wei-liang ZHAO Xue-yao LIU Yu-qi LI Wei-qiang LIU Da-sheng WANG Yan-ping LU Ai-ping | 2018 | Chinese Journal of Integrative Medicine2018，24，7： | 8 |
| 6 | Pituitary tumor transforming gene: a novel therapeutic target for glioma treatment显示文摘有强壮的增长和 angiogenesis 能力的 Glioma 是在中央神经系统的最普通、恶意的主要肿瘤。垂体肿瘤转变基因(PTTG ) 在垂体肿瘤被发现,并且在房间增长,房间周期,房间 apoptosis,和 angiogenesis 起重要作用。然而,在 glioma 的 PTTG 的角色仍然不完全地被调查。这里,我们探索了在 PTTG 和 glioma 等级,以及微容器的密度(MVD ) 之间的关联。另外, siRNA 包括 U87MG, U251,和 SHG44 在 glioma 房间线习惯于沉默 PTTG 表示。房间增长, apoptosis,侵略,和 angiogenesis 在 vitro 并且在 vivo 被学习。我们的结果证明那 PTTG 表情在 glioma 是显著地起来调整的,并且与 glioma 等级和 MVD 有积极关联。PTTG 的 Silencing 禁止了 glioma 房间增长,迁居 / 侵略,和 angiogenesis,导致了房间 apoptosis,压制了房间侵略,并且逮捕了在 G0/G1 舞台的房间周期。PTTG 的 Silencing 能也在 vivo 禁止肿瘤生长,侵略,和 angiogenesis。我们的数据显示 PTTG 可能是为 glioma 处理的一个潜在的目标。 | Lishan Cui Songbai Xu Zhengmao Song Gang Zhao Xiaoqian Liu Yuwen Song | 2015 | Acta Biochimica et Biophysica Sinica2015，47，6： | 6 |
| 7 | Hydrogen production from coke oven gas over LiNi/γ-Al_2O_3 catalyst modified by rare earth metal oxide in a membrane reactor显示文摘The performance of LiNi/γ-Al2O3 catalysts modified by rare earth metal oxide(La2O3 or CeO2 ) packed on BCFNO membrane reactor was discussed for the partial oxidation of methane(POM) in coke oven gas(COG) at 875 C. The NiO/γ-Al2O3 catalysts with different amounts of La2O3 and CeO2 were prepared with the same preparation method and under the same condition in order to compare the reaction performance(oxygen permeation,CH 4 conversion,H 2 and CO selectivity) on the membrane reactor. The results show that the oxygen permeation flux increased significantly with LiNiREO x /γ-Al2O3(RE = La or Ce) catalysts by adding the element of rare earth especially the Ce during the POM in COG. Such as,the Li15wt%CeO2 9wt%NiO/γ-Al2O3 catalyst with an oxygen permeation flux of 24.71 ml cm 2 min 1 and a high CH 4 conversion was obtained in 875 C. The resulted high oxygen permeation flux may be due to the added Ce that inhibited the strong interaction between Ni and Al2O3 to form the NiAl2O4 phase. In addition,the introduction of Ce leads up to an important property of storing and releasing oxygen. | Zhibin Yang Yunyan Zhang Weizhong Ding Yuwen Zhang Peijun Shen Yuding Zhou Yong Liu Shaoqing Huang Xionggang Lu | 2009 | Journal of Natural Gas Chemistry2009，18，4： | 6 |
| 8 | Genome wide abnormal DNA methylome of human blastocyst in assisted reproductive technology显示文摘Proper reprogramming of parental DNA methylomes is essential for mammalian embryonic development.However,it is unknown whether abnormal methylome reprogramming occurs and is associated with the failure of embryonic development.Here we analyzed the DNA methylomes of 57 blastocysts and 29 trophectoderm samples with different morphological grades during assisted reproductive technology(ART) practices.Our data reveal that the global methylation levels of high-quality blastocysts are similar(0.30 ± 0.02,mean ± SD).while the methylation levels of low-quality blastocysts are divergent and away from those of high-quality blastocysts.The proportion of blastocysts with a methylation level falling within the range of 0.30 ± 0.02 in different grades correlates with the live birth rate for that grade.Moreover,abnormal methylated regions are associated with the failure of embryonic development.Furthermore,we can use the methylation data of cells biopsied from trophectoderm to predict the blastocyst methylation level as well as to detect the aneuploidy of the blastocysts.Our data indicate that global abnormal methylome reprogramming often occurs in human embryos,and suggest that DNA methylome is a potential biomarker in blastocyst selection in ART. | Guoqiang Li Yang Yu Yong Fan Congru Li Xiaocui Xu Jialei Duan Rong Li Xiangjin Kang Xin Ma Xuepeng Chen Yuwen Ke Jie Yan Ying Lian Ping Liu Yue Zhao Hongcui Zhao Yaoyong Chen Xiaofang Sun Jianqiao Liu Jie Qiao Jiang Liu | 2017 | Journal of Genetics and Genomics2017，44，10： | 5 |
| 9 | Partial oxidation of simulated hot coke oven gas to syngas over Ru-Ni/Mg(Al)O catalyst in a ceramic membrane reactor显示文摘Hydrogen amplification from simulated hot coke oven gas(HCOG) was investigated in a BaCo 0.7 Fe 0.2 Nb 0.1 O 3 δ(BCFNO) membrane reactor combined with a Ru-Ni/Mg(Al) O catalyst by the partial oxidation of hydrocarbon compounds under atmospheric pressure. Under optimized reaction conditions,the dense oxygen permeable membrane had an oxygen permeation flux around 13.3 ml/(cm 2 min) . By reforming of the toluene and methane,the amount of H 2 in the reaction effluent gas was about 2 times more than that of original H2 in simulated HCOG. The Ru-Ni/Mg(Al) O catalyst used in the membrane reactor possessed good catalytic activity and resistance to coking. After the activity test,a small amount of whisker carbon was observed on the used catalyst,and most of them could be removed in the hydrogen-rich atmosphere,implying that the carbon deposition formed on the catalyst might be a reversible process. | Hongwei Cheng Xionggang Lu Xu Liu Yuwen Zhang Weizhong Ding | 2009 | Journal of Natural Gas Chemistry2009，18，4： | 4 |
| 10 | Establishment of a dmp based maternal haploid induction system for polyploid Brassica napus and Nicotiana tabacum显示文摘Doubled haploid(DH) technology is used to obtain homozygous lines in a single generation, a technique that significantly accelerates the crop breeding trajectory. Traditionally, in vitro culture is used to generate DHs, but this technique is limited by species and genotype recalcitrance. In vivo haploid induction(HI) through seed is widely and efficiently used in maize and was recently extended to several other crops. Here we show that in vivo HI can be triggered by mutation of DMP maternal haploid inducer genes in allopolyploid(allotetraploid) Brassica napus and Nicotiana tabacum. We developed a pipeline for selection of DMP orthologs for clustered regularly interspaced palindromic repeats mutagenesis and demonstrated average amphihaploid induction rates of2.4% and 1.2% in multiple B. napus and N. tabacum genotypes, respectively. These results further confirmed the HI ability of DMP gene in polyploid dicot crops. The DMP-HI system offers a novel DH technology to facilitate breeding in these crops. The success of this approach and the conservation of DMP genes in dicots suggest the broad applicability of this technique in other dicot crops. | Yu Zhong Yuwen Wang Baojian Chen Jinchu Liu Dong Wang Mengran Li Xiaolong Qi Chenxu Liu Kim Boutilier Shaojiang Chen | 2022 | Journal of Integrative Plant Biology2022，64，6： | 4 |
| 11 | siRNA targeting stathmin inhibits invasion and enhances chemotherapy sensitivity of stem cells derived from glioma cell lines显示文摘Glioma 高度是大多数之一 angiogenic 肿瘤,和 glioma 干细胞(GSC ) 为到化疗和放射疗法的抵抗负责,以及在操作以后的复发。Stathmin 为有丝分裂是实质的并且在导出 glioma 的 endothelial 房间的增长和移植起一个重要作用。然而,在 stathmin 和 GSC 之间的关系不完全地被理解。这里,我们从 glioma 房间线 U87MG 和 U251 孤立 GSC,然后使用了为 silencing 指向 stathmin 的 siRNA。我们证明 stathmin 的 silencing 压制了增长,增加了 apoptosis 率,并且逮捕了在在 GSC 的 G2/M 阶段的房间周期。在 GSC 的 stathmin 的 Silencing 也导致了禁止了迁居 / 侵略以及 vasculogenic 的能力模仿。到 temozolomide 的 GSC 的危险性被 stathmin silencing 也提高。我们的调查结果为 glioma 处理在 GSC 作为一个潜在的目标建议 stathmin。 | Yuwen Song Luyan Mu Xuezhe Han Xiaoqian Liu Songbin Fu | 2014 | Acta Biochimica et Biophysica Sinica2014，46，12： | 4 |
| 12 | Recent Developments of Modulation and Control for High-Power Current-Source-Converters Fed Electric Machine Systems显示文摘The pulse-width-modulated(PWM)current-source converters(CSCs)fed electric machine systems can be considered as a type of high reliability energy conversion systems,since they work with the long-life DC-link inductor and offer high fault-tolerant capability for short-circuit faults.Besides,they provide motor friendly waveforms and four-quadrant operation ability.Therefore,they are suitable for high-power applications of fans,pumps,compressors and wind power generation.The purpose of this paper is to comprehensively review recent developments of key technologies on modulation and control of high-power(HP)PWM-CSC fed electric machines systems,including reduction of low-order current harmonics,suppression of inductor–capacitor(LC)resonance,mitigation of common-mode voltage(CMV)and control of modular PWM-CSC fed systems.In particular,recent work on the overlapping effects during commutation,LC resonance suppression under fault-tolerant operation and collaboration of modular PMW-CSCs are described.Both theoretical analysis and some results in simulations and experiments are presented.Finally,a brief discussion regarding the future trend of the HP CSC fed electric machines systems is presented. | Pengcheng Liu Zheng Wang Sanmin Wei Yuwen Bo Shaoning Pu | 2020 | CES Transactions on Electrical Machines and Systems2020，4，3： | 3 |
| 13 | All-optical logic devices based on black arsenic-phosphorus with strong nonlinear optical response and high stability显示文摘The Kerr nonlinearity in two-dimensional(2D)nanomaterials is emerging as an appealing and intriguing research area due to their prominent light processing,modulation,and manipulation abilities.In this contribution,2D black arsenic-phosphorus(B-AsP)nanosheets(NSs)were applied in nonlinear photonic devices based on spatial self-phase modula-tion(SSPM)method.By applying the Kerr nonlinearity in 2D B-AsP,an all-optical phase-modulated system is proposed to realize the functions of“on”and“off”in all-optical switching.By using the same all-optical phase-modulated system,another optical logic gate is proposed,and the logical“or”function is obtained based on the 2D B-AsP NSs dispersions.Moreover,by using the SSPM method,a 2D B-AsP/SnS_(2) hybrid structure is fabricated,and the result illustrates that the hybrid structure possesses the ability of the unidirectional nonlinear excitation,which helps in obtaining the function of spatial asymmetric light propagation.This function is considered an important prerequisite for the realization of diode functionalization,which is believed to be a factor in important basis for the design of isolators as well.The initial investig-ations indicate that 2D B-AsP is applicable for designing optical logical devices,which can be considered as an import-ant development in all-optical information processing. | Leiming Wu Taojian Fan Songrui Wei Yijun Xu Ye Zhang Dingtao Ma Yiqing Shu Yuanjiang Xiang Jun Liu Jianqing Li Krassimir Panajotov Yuwen Qin Han Zhang | 2022 | Opto-Electronic Advances2022，5，1： | 3 |
| 14 | Rapid isolation and immune profiling of SARS-CoV-2 specific memory B cell in convalescent COVID-19 patients via LIBRA-seq显示文摘B cell response plays a critical role against SARS-CoV-2 infection.However,little is known about the diversity and frequency of the paired SARS-CoV-2 antigen-specific BCR repertoire after SARS-CoV-2 infection.Here,we performed single-cell RNA sequencing and VDJ sequencing using the memory and plasma B cells isolated from five convalescent COVID-19 patients,and analyzed the spectrum and transcriptional heterogeneity of antibody immune responses.Via linking BCR to antigen specificity through sequencing(LIBRA-seq),we identified a distinct activated memory B cell subgroup(CD11c^(high) CD95^(high))had a higher proportion of SARS-CoV-2 antigen-labeled cells compared with memory B cells.Our results revealed the diversity of paired BCR repertoire and the non-stochastic pairing of SARS-CoV-2 antigen-specific immunoglobulin heavy and light chains after SARS-CoV-2 infection.The public antibody clonotypes were shared by distinct convalescent individuals.Moreover,several antibodies isolated by LIBRA-seq showed high binding affinity against SARS-CoV-2 receptor-binding domain(RBD)or nucleoprotein(NP)via ELISA assay.Two RBD-reactive antibodies C14646P3S and C2767P3S isolated by LIBRA-seq exhibited high neutralizing activities against both pseudotyped and authentic SARS-CoV-2 viruses in vitro.Our study provides fundamental insights into B cell response following SARS-CoV-2 infection at the single-cell level. | Bing He Shuning Liu Yuanyuan Wang Mengxin Xu Wei Cai Jia Liu Wendi Bai Shupei Ye Yong Ma Hengrui Hu Huicui Meng Tao Sun Yanling Li Huanle Luo Mang Shi Xiangjun Du Wenjing Zhao Shoudeng Chen Jingyi Yang Haipeng Zhu Yusheng Jie Yuedong Yang Deyin Guo Qiao Wang Yuwen Liu Huimin Yan Manli Wang Yao-Qing Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 2 |
| 15 | GLRT-based generalized direction detector in partially homogeneous environment显示文摘Dear editor,Target detection is a basic function for any radar system (1)For the problem of generalized direction detection, two adaptive detectors were proposed in [2], according to the generalized likelihood ratio test (GLRT). For the above problem,under the signal-presence hypothesis, the rows and columns of a rank-one matrix-valued signal belong to known subspaces;however, the corresponding coordinates are unknown. | Weijian LIU Fei GAO Yuwen LUO Jun LIU Yongliang WANG | 2019 | Science China(Information Sciences)2019，62，10： | 2 |
| 16 | Total conductivity,oxygen permeability and stability of perovskite-type oxide BaCo_(0.7)Fe_(0.2)Nb_(0.1)O_(3-δ)显示文摘The total conductivity,oxygen sorption property,oxygen permeability and stability of pure perovskite-type oxide BaCo0.7Fe0.2Nb0.1O3-δ (BCFNO) in real operating conditions were investigated. Its total conductivity was measured to be 3.6 S·cm-1 at 600°C. Though the total conductivity of the BCFNO membrane is much smaller than that of the Ba0.5Sr0.5Co0.8Fe0.2O3-δ (BSCFO) membrane,the oxygen permeability of the BCFNO membrane is similar to that of the BSCFO membrane. SEM observation and EDX analysis of the BCFNO and BSCFO membranes indicated that no segregation of metal ions was found for the used BCFNO membrane while the original perovskite phase of BSCFO decomposed under the experimental condition. The experimental results of oxygen permeability and stability were consistent with the analysis on the oxygen sorption property of perovskites. | Zhang, Yuwen Liu, Yong Wang, Chenglei Yang, Zhibin Ding, Weizhong Lu, Xionggang | 2009 | Rare Metals2009，28，2： | 2 |
| 17 | Influence of alloplasm on resistance to wheat scab显示文摘Using alloplasmic wheat as test materials, the genetic effect of cytoplasm on wheat scab resistance was studied by three methods including the determination of resistance of explant calli to Gibberella zeae toxin, the identification of inoculating single fixed position and the identification of field resistance. The results showed that among 'the homonucleo_heterocytoplasmic lines', resistance of calli to G. zeae toxin was different; the difference of cytoplasmic effect among different nucleoplasm combinations showed certain nucleoplasm interaction relation. Resistance of hybrid F 1 plants of reciprocal cross between alloplasmic line and disease-sensitive wheat variety to wheat scab was of clear difference. Hybrid F 1 of reciprocal cross between Ae. ventricosa cytoplasmic wheat and disease-sensitive or -resistant wheat variety all exhibited fine resistance effect of alloplasm. Compared with general common wheat variety, most alloplasmic wheats tested have stronger resistance. A few selected lines having Ae. ventricosa cytoplasm have a strong and stable resistance to scab. They can be a finer new resistant source. | Wu Yuwen Ren Shuxin Liu Chunguang Hou Ning Zhang Cuilan Zhang Yan | 1998 | Chinese Science Bulletin1998，43，12： | 2 |
| 18 | Genome-wide analyses on transcription factors and their potential microRNA regulators involved in maize male fertility显示文摘Anther development is a programmed biological process crucial to plant male reproduction. Genomewide analyses on the functions of transcriptional factor(TF) genes and their microRNA(miRNA) regulators contributing to anther development have not been comprehensively performed in maize. Here, using published RNA-Seq and small RNA-Seq(sRNA-Seq) data from maize anthers at ten developmental stages in three genic male-sterility(GMS) mutants(ocl4, mac1, and ms23) and wild type W23, as well as newly sequenced maize anther transcriptomes of ms7-6007 and lob30 GMS mutants and their WT lines, we analyzed and found 1079 stage-differentially expressed(stage-DE) TF genes that can be grouped into six(premeiotic, meiotic, postmeiotic, premeiotic-meiotic, premeiotic-postmeiotic, and meiotic-postmeiotic clusters) expression clusters. Functional enrichment combined with cytological and physiological analyses revealed specific functions of genes in each expression cluster. In addition, 118 stage-DE miRNAs and99 miRNA-TF gene pairs were identified in maize anthers. Further analyses revealed the regulatory roles of zma-miR319 and zma-miR159 as well as ZmMs7 and ZmLOB30 on ZmGAMYB expression. Moreover,ZmGAMYB and its paralog ZmGAMYB-2 were demonstrated as novel maize GMS genes by CRISPR/Cas9 knockout analysis. These results extend our understanding on the functions of miRNA-TF gene regulatory pairs and GMS TF genes contributing to male fertility in plants. | Ziwen Li Taotao Zhu Shuangshuang Liu Yilin Jiang Haoyun Liu Yuwen Zhang Ke Xie Jinping Li Xueli An Xiangyuan Wan | 2021 | The Crop Journal2021，9，6： | 2 |
| 19 | Apoptosis of A549 cells by small interfering RNA targeting survivin delivery using poly-β-amino ester/guanidinylated O-carboxymethyl chitosan nanoparticles显示文摘Gene-based therapeutics has emerged as a promising approach for human cancer therapy. Among a variety of non-viral vectors, polymer vectors are particularly attractive due to their safety and multivalent groups on their surface. This study focuses on guanidinylated O-carboxymethyl chitosan(GOCMCS) along with poly-β-amino ester(PBAE) for si RNA delivery. Binding efficiency of PBAE/si RNA/GOCMCS nanoparticles were characterized by gel electrophoresis. The si RNA-loaded nanoparticles were found to be stable in the presence of RNase A, serum and BALF respectively. Fine particle fraction(FPF) which was determined by a two-stage impinger(TSI) was 57.8% ± 2.6%. The particle size and zeta potential of the nanoparticles were 153.8 ± 12.54 nm and + 12.2 ± 4.94 m V. In vitro cell transfection studies were carried out with A549 cells. The cellular uptake was significantly increased. When the cells were incubated with si Survivin-loaded nanoparticles, it could induce 26.83% ± 0.59% apoptosis of A549 cells and the gene silencing level of survivin expression in A549 cells were 30.93% ± 2.27%. The results suggested that PBAE/GOCMCS nanoparticle was a very promising gene delivery carrier. | Yue Tang Yun Liu Yuwen Xie Jing Chen Yushun Dou | 2020 | Asian Journal of Pharmaceutical Sciences2020，15，1： | 2 |
| 20 | The add-on effect of a Chinese herbal formula for patients with resistant hypertension: study protocol for a pilot cohort study显示文摘BACKGROUND: Despite a recent American Heart Association(AHA) consensus statement emphasizing the importance of resistant hypertension(RH), its control is still a challenge for conventional medicine. The Chinese herbal formula, Qutan Huayu Fang, has been used effectively to assist antihypertensive agents in blood pressure control, but its effect for RH patients is still unclear. This pilot study aims to explore the effects of taking the formula in addition to antihypertensive medication in the management of RH.METHODS/DESIGN: A prospective cohort study will be conducted in two first-class hospitals of traditional Chinese medicine(TCM). Eligible RH patients will be classified as the experimental group(n = 100) and the control group(n = 100) based on the interventions they receive. Participants taking antihypertensive agents and the Chinese herbal formula will be in the experimental group and those taking antihypertensive agents alone will be in the control group. The whole study will last 24 weeks, including an 8-week observation and follow-up at 24 weeks. The primary outcomes, assessed against patient baseline conditions, will be the reduction of systolic blood pressure and diastolic blood pressure as well as changes in TCM symptoms and signs. These outcomes will be assessed at weeks 2, 4, 6, and 8. The reductions of blood pressure will also be assessed at week 24. Cardiac events and mortality rate will be secondary outcomes and will be assessed at weeks 8 and 24. Any adverse reactions will be recorded during the study. The causal inference method will be used to assess the effectiveness of the inclusion of TCM herbal medicine in the management of patients with RH.DISCUSSION: This study will determine whether the Chinese herbal formula is helpful for RH patients treated with antihypertensive agents and the findings will provide a basis for further confirmatory studies. | Ya Yuwen Yu-qi Liu Yan-ping Wang Jin-gang Dai Da-sheng Liu Yue-xi Wang Xue-jie Han | 2015 | Journal of Integrative Medicine2015，13，2： | 2 |
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