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您的检索式：作者名="Yunyang Luo"
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| 1 | Rapid accuracy determining DNA purity and concentration in heavy oils by spectrophotometry methods显示文摘DNA analysis is the core of biotechnology applied in petroleum resources and engineering. Traditionally accurate determination of DNA purity and concentration by spectrometer is the first and critical step for downstream molecular biology research. In this study, three different spectrophotometry methods, BPM, NDTT and NPMTTZ were compared for their performance in determining DNA concentration and purity in 32 oil samples, and molecule methods like quantitative real-time PCR (qPCR) and high-throughput sequence were also performed to help assess the accuracy of the three methods in determining DNA concentration and purity. For ordinary heavy oil (OHO), extra heavy oil (EHO) and super heavy oil (SHO), the characteristics of high viscosity (η), density (ρ) and resin plus asphaltene content will affect the DNA extraction and UV determination. The DNA concentration was decreased as density increased: OHO (11.46 ± 18.34 ng/μL), EHO (6.68 ± 9.67 ng/μL) and SHO (6.20 ± 7.83 ng/μL), and the DNA purity was on the reverse: OHO (1.31 ± 0.27), EHO (1.54 ± 0.20), and SHO (1.83 ± 0.32). The results suggest that spectrophotometry such as BPM and NPMTTZ are qualitatively favorite methods as the quick non-consumable methods in determining DNA concentration and purity of medium oil and heavy oil. | YunYang Wan HongMei Mu Na Luo JianPing Yang Yan Tian Ning Hong HaiLiang Dong | 2023 | Petroleum Science2023，20，6： | 0 |
| 2 | Determination of Double Beta Decay Half-Life of 136Xe with the PandaX-4T Natural Xenon Detector显示文摘Precise measurement of two-neutrino double beta decay(DBD)half-life is an important step for the searches of Majorana neutrinos with neutrinoless double beta decay.We report the measurement of DBD half-life of 136xe using the Pandax-4T dual-phase Time Projection Chamber(TPC)with 3.7-tonne natural xenon and the first 94.9-day physics data release. | Lin Si Zhaokan Cheng Abdusalam Abdukerim Zihao Bo Wei Chen Xun Chen Yunhua Chen Chen Cheng Yunshan Cheng Xiangyi Cui Yingjie Fan Deqing Fang Changbo Fu Mengting Fu Lisheng Geng Karl Giboni Linhui Gu Xuyuan Guo Ke Han Changda He Jinrong He Di Huang Yanlin Huang Zhou Huang Ruquan Hou Xiangdong Ji Yonglin Ju Chenxiang Li Jiafu Li Mingchuan Li Shu Li Shuaijie Li Qing Lin Jianglai Liu Xiaoying Lu Lingyin Luo Yunyang Luo Wenbo Ma Yugang Ma Yujun Mao Yue Meng Nasir Shaheed Xiaofeng Shang Xuyang Ning Ningchun Qi Zhicheng Qian Xiangxiang Ren Changsong Shang Guofang Shen Wenliang Sun Andi Tan Yi Tao Anqing Wang Meng Wang Qiuhong Wang Shaobo Wang Siguang Wang Wei Wang Xiuli Wang Zhou Wang Yuehuan Wei Mengmeng Wu Weihao Wu Jingkai Xia Mengjiao Xiao Xiang Xiao Pengwei Xie Binbin Yan Xiyu Yan Yong Yang Chunxu Yu Jumin Yuan Ying Yuan Zhe Yuan Dan Zhang Minzhen Zhang Peng Zhang Shibo Zhang Shu Zhang Tao Zhang Li Zhao Qibin Zheng Jifang Zhou Ning Zhou Xiaopeng Zhou Yong Zhou | 2023 | Research2023，，2： | 0 |
| 3 | Visualizing the nucleoplasmic maturation of human pre-60S ribosomal particles显示文摘Eukaryotic ribosome assembly is a highly orchestrated process that involves over two hundred protein factors.After early assembly events on nascent rRNA in the nucleolus,pre-60S particles undergo continuous maturation steps in the nucleoplasm,and prepare for nuclear export.Here,we report eleven cryo-EM structures of the nuclear pre-60S particles isolated from human cells through epitope-tagged GNL2,at resolutions of 2.8–4.3Å.These high-resolution snapshots provide fine details for several major structural remodeling events at a virtual temporal resolution.Two new human nuclear factors,L10K and C11orf98,were also identified.Comparative structural analyses reveal that many assembly factors act as successive place holders to control the timing of factor association/dissociation events.They display multi-phasic binding properties for different domains and generate complex binding inter-dependencies as a means to guide the rRNA maturation process towards its mature conformation.Overall,our data reveal that nuclear assembly of human pre-60S particles is generally hierarchical with short branch pathways,and a few factors display specific roles as rRNA chaperones by confining rRNA helices locally to facilitate their folding,such as the C-terminal domain of SDAD1. | Yunyang Zhang Xiaomeng Liang Sha Luo Yan Chen Yu Li Chengying Ma Ningning Li Ning Gao | 2023 | Cell Research2023，33，11： | 0 |
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