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| 1 | Improved Base Editor for Efficiently Inducing Genetic Variations in Rice with CRISPR/Cas9- Guided Hyperactive hAID Mutant显示文摘 | Bin Ren Fang Yan Yongjie Kuang Na Li Dawei Zhang Xueping Zhou Honghui Lin Huanbin Zhou | 2018 | Molecular Plant2018，11，4： | 46 |
| 2 | An Effective Method for Total RNA Isolation from Bamboo显示文摘Trizol reagent was used for RNA isolation from fresh leaves of Dendrocalamopsis oldhami, Bambusa ventricosa and Phyllostachys aureosulcata cv. Pekinensis. The extracted RNA from leaves had the normal ultraviolet absorption, the value of OD260/OD280 varied between 1.8-2.0. The 28s rRNA was more than two times brighter than 18s rRNA in electrophoresis. These results indicated that the total RNA was complete and not degraded. According to our experiments, RNA could be obtained simply with this method, and can be used for molecular manipulation such as cDNA synthesis and gene cloning. | GAO Zhimin LI Xueping LI Lubin PENG Zhenhua | 2006 | Chinese Forestry Science and Technology2006，5，3： | 39 |
| 3 | Base-Editing-Mediated Artificial Evolution of OsALS1 In Planta to Develop Novel Herbicide-Tolerant Rice Germplasms显示文摘Recently developed CRISPR-mediated base editors,which enable the generation of num erous nucleotide changes in target genomic regions,have been widely adopted for gene correction and generation of crop germ plasms containing im portant gain-of-function genetic variations.How ever,to engineer target genes with unknown functional SNPs remains challenging.To address this issue,we present here abase-e diting-mediated gene evolution(BEMGE)m ethod,employing both Cas9n-based cytosine and adenine base editors as well as a single-guide RNA(sgRNA)library tiling the full-length coding region,for developing novel rice germ plasm swith mutations in any endogenous gene.To this end,OsALS1 was artificially evolved in rice cells using BEMGE through both Agrobacterium-mediated and particle-bom bardment-mediated transform ation.Four different types of amino acid substitutions in the evolved OsALS1,derived from two sites that have never been targeted by natural or human selection during rice dom estication,were identified,conferring varying levels of tolerance to the herbicide bispyribac-sodium.Furtherm ore,the P171F substitution identified in a strong OsALS1 allele was quickly introduced into the commercial rice cultivar Nangeng 46 through precise base editing w ith the corresponding base editor and sgRNA.Collectively,these data indicate great potential of BEMGE in creating important genetic variants of target genes for crop improvement. | Yongjie Kuang Shaofang Li Bin Ren Fang Yan Carl Spetz Xiangju Li Xueping Zhou Huanbin Zhou | 2020 | Molecular Plant2020，13，4： | 37 |
| 4 | Chinese Stroke Association guidelines for clinical management of cerebrovascular disorders:executive summary and 2019 update of clinical management of stroke rehabilitation显示文摘Stroke is the third leading cause of long-term disability.Effective stroke rehabilitation should be provided to patients with a moderate or severe stroke so as to achieve functional recovery.Also,stroke rehabilitation is moving towards more intense therapy models that incorporate technologies such as robotics and computer games.Evidence-based guidelines are useful for promoting the best possible clinical practices.This guideline summarises the modes of stroke rehabilitation in China-three-stage rehabilitation network,rehabilitation of stroke dysfunction,training for activities of daily living,stroke complications and rehabilitation care.The aim of this guideline was to provide a synopsis of best clinical practices in the stroke rehabilitation of adult patients in China. | Tong Zhang Jun Zhao Xueping Li Yulong Bai Baojun Wang Yun Qu Bingjie Li Shengjie Zhao Chinese Stroke Association Stroke Council Guideline Writing Committee | 2020 | Stroke & Vascular Neurology2020，5，3： | 38 |
| 5 | A Complete Analytic Model for Fault Diagnosis of Power Systems显示文摘现代大规模互联电网提高了电力系统运行的稳定性和经济性,同时也给电网的故障诊断增加了难度。对电网保护配置和断路器动作规则进行深入分析,提出一种新的电网故障诊断模型——完全解析模型,并为基于此模型的故障诊断提出一种实用有效的求解方法。在完全解析模型中,故障假说(即可疑故障设备的故障情况)与保护、断路器的动作情况及拒动和误动情况一同表示成逻辑变量,而保护配置和断路器动作规则以逻辑方程组形式进行充分表达,逻辑方程组的每个解析解对应一个故障模式(设备故障状态和保护动作、断路器跳闸状态及拒动/误动情况),是对故障情形的完整解释。本模型对故障诊断规则和保护、断路器的动作逻辑进行完全解析,并完整保留了故障设备、保护动作和断路器跳闸之间的耦合关系,克服了已有解析模型的缺陷。算例表明,保留完整耦合关系的完全解析模型可有效提高故障诊断的准确性和容错能力。 | LIU Daobing GU Xueping LI Haipeng | 2011 | 中国电机工程学报2011，31，34： | 33 |
| 6 | Virus-induced gene silencing and its application in plant functional genomics显示文摘Virus-induced gene silencing is regarded as a powerful and efficient tool for the analysis of gene function in plants because it is simple, rapid and transformation-free. It has been used to perform both forward and reverse genetics to identify plant functional genes. Many viruses have been developed into virus-induced gene silencing vectors and gene functions involved in development, biotic and abiotic stresses, metabolism, and cellular signaling have been reported. In this review, we discuss the development and application of virus-induced gene silencing in plant functional genomics. | HUANG ChangJun QIAN YaJuan LI ZhengHe ZHOU XuePing | 2012 | Science China(Life Sciences)2012，55，2： | 20 |
| 7 | Cas9-NG Greatly Expands the Targeting Scope of the Genome-Editing Toolkit by Recognizing NG and Other Atypical PAMs in Rice显示文摘CRISPR technologies enabling precise genome manipulation are valuable for gene function studies and molecular crop breeding. However, the requirement of a protospacer adjacent motif (PAM)y such as NGG and TTN, for Cas protein recognition restricts the selection of targetable genomic loci in practical applications of CRISPR technologies. Recently Cas9-NG, which recognizes a minimal NG PAM, was reported to expand the targeting space of genome editing in human cells, but it remains unclear whether this Cas9 variant can be used in plants. In this study, we evaluated the nuclease activity of Cas9-NG toward various NGN PAMs by targeting endogenous genes in transgenic rice. We found that Cas9-NG edits all NGG, NGA, NGT, and NGC sites with impaired activity, while the gene-edited plants were dominated by monoallelic mutations. Cas9-NG-engineered base editors were then developed and used to generate O s B Z R I gainof- function plants that can not be created by other available Cas9-engineered base editors. Moreover, we showed that a Cas9-NG-based transcriptional activator efficiently upregulated the expression of endogenous target genes in rice. In addition, we discovered that Cas9-NG recognizes NAC, NTG, NTT, and NCG apart from NG PAM. Together, these findings demonstrate that Cas9-NG can greatly expand the targeting scope of genome-editing tools, showing great potential for targeted genome editing, base editing, and genome regulation in plants. | Bin Ren Lang Liu Shaofang Li Yongjie Kuang Jingwen Wang Dawei Zhang Xueping Zhou Honghui Lin Huanbin Zhou | 2019 | Molecular Plant2019，12，7： | 18 |
| 8 | Oxidative stress, mitochondrial damage and neurodegenerative diseases显示文摘Oxidative stress and mitochondrial damage have been implicated in the pathogenesis of several neurodegenerative diseases, including Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis. Oxidative stress is characterized by the overproduction of reactive oxygen species, which can induce mitochondrial DNA mutations, damage the mitochondrial respiratory chain, alter membrane permeability, and influence Ca2+ homeostasis and mitochondrial defense systems. All these changes are implicated in the development of these neurodegenerative diseases, mediating or amplifying neuronal dysfunction and triggering neurodegeneration. This paper summarizes the contribution of oxidative stress and mitochondrial damage to the onset of neurodegenerative diseases and discusses strategies to modify mitochondrial dysfunction that may be attractive therapeutic interventions for the treatment of various neurodegenerative diseases. | Chunyan Guo Li Sun Xueping Chen Danshen Zhang | 2013 | Neural Regeneration Research2013，8，21： | 15 |
| 9 | Impact of mechanical circulatory support and immunomodulation therapy on outcome of patients with fulminant myocarditis: Chinese registry of fulminant myocarditis显示文摘Dear Editor,Patients presenting with acute myocarditis and sudden hemodynamic instability (termed fulminant myocarditis [FM]) still have a high mortality and need for heart transplantation, up to 28% at 60 days.1,2,3 Recent scientific statements and expert opinion consensus suggests early use of temporary mechanical circulatory supports (t-MCS).3,4 Specifically, Chinese scientific statement proposed an extensive use of t-MCS combined with immunoregulatory therapy (IT),4 although formal trials are lacking. We present a multicenter, retrospective study to compare the outcome of patients who were treated with t-MCS and IT vs. patients who didn’t receive these treatments. We included patients with the diagnosis of FM based on the presence of viral prodromal signs/symptoms followed by acute onset of severe heart failure (HF) without other relevant differential diagnosis or pre-existing cardiac disorders. Patients who received both t-MCS and IT during hospitalization were classified as t-MCS+IT group. | Ning Zhou Yuhua Zhao Jiangang Jiang Lan Shen Junming Li Jing Wan Xueping Ma Jing Zhang Enrico Ammirati Dao Wen Wang | 2021 | Signal Transduction and Targeted Therapy2021，6，11： | 14 |
| 10 | Molecular characterization of Tomato yellow leaf curl China virus and its satellite DNA isolated from tobacco显示文摘Virus isolates, Y8, Y36 and Y38, were obtained from tobacco plants showing leaf curl symptoms in Honghe, Yunnan Province. In reactions with 14 monoclonal antibod-ies raised against Begomovirus particles, Y8, Y36 and Y38had similar antigenic reaction in TAS-ELISA as Tomato yel-low leaf curl China virus (TYLCCNV). The complete DNA-A nucleotide sequences of Y8, Y36 and Y38 were determinedand they contain 2727, 2730 and 2730 nucleotides, respec-tively. Each of the DNA-A sequences has a typical Bego-movirus genome organization encoding 6 ORFs with 2 ORFs [AV1(CP) and AV2] in virion-sense DNA and 4 ORFs (AC1to AC4) in complementary-sense DNA. Comparisons withtotal DNA-A, intergenic region and deduced amino acid se-quences of individual ORFs show that Y8, Y36 and Y38 are isolates of TYLCCNV. Satellite DNA molecules (DNAb) were found to be associated with Y8, Y36 and Y38, which consist of 1338, 1339 and 1338 nucleotides, respectively. Compari-sons show that these DNAb molecules share 98%—99% se-quence identities on nucleotide level and have a commonORF (designated C1) encoding 126 amino acids on the com-plementary strand. | XIE Yan, ZHOU Xueping & LI Guixin Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China Correspondence should be addressed to Zhou Xueping (e-mail: zzhou@zju.edu.cn) | 2003 | Chinese Science Bulletin2003，48，8： | 13 |
| 11 | Quasi-solid-state nanocrystalline TiO2 solar cells using gel network polymer electrolytes based on polysiloxanes显示文摘A quasi-solid-state dye-sensitized nanocrystal-line porous TiO2 film solar cell was fabricated using a novel gel network polymer electrolyte based on polysiloxanes with both polyethylene oxide internal plasticized side chains and quaternary ammonium groups. The cell exhibited better photoelectrical conversion performance under 60 mW/cm2 irradiation. The short photocurrent (Isc) of 5.0 mA/cm2 and open voltage (Voc) of 0.68 V were achieved, and the energy conversion efficiency (h ) and fill factor (ff) were 3.4% and 0.60, respectively. | LI Weiying, KANG Junjie, LI Xueping, FANG Shibi, LIN Yuan, WANG Guiqiang & XIAO Xurui Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China | 2003 | Chinese Science Bulletin2003，48，7： | 11 |
| 12 | The fabrication of biomimetic biphasic CAN-PAC hydrogel with a seamless interfacial layer applied in osteochondral defect repair显示文摘Cartilage tissue engineering based on biomimetic scaffolds has become a rapidly developing strategy for repairing cartilage defects. In this study, a biphasic CAN-PAC hydrogel for osteochondral defect(OCD)regeneration was fabricated based on the density difference between the two layers via a thermally reactive,rapid cross-linking method. The upper hydrogel was cross-linked by CSMA and NIPAm, and the lower hydrogel was composed of PECDA, AAm and PEGDA. The interface between the two layers was first grafted by the physical cross-linking of calcium gluconate and alginate, followed by the chemical cross-linking of the carbon-carbon double bonds in the other components. The pore sizes of the upper and lower hydrogels were ~ 187.4 and ~ 112.6 μm, respectively. The moduli of the upper and lower hydrogels were ~ 0.065 and~ 0.261 MPa. This prepared bilayer hydrogel exhibited the characteristics of mimetic composition, mimetic structure and mimetic stiffness, which provided a microenvironment for sustaining cell attachment and viability. Meanwhile, the biodegradability and biocompatibility of the CAN-PAC hydrogel were examined in vivo. Furthermore, an osteochondral defect model was developed in rabbits, and the bilayer hydrogels were implanted into the defect. The regenerated tissues in the bilayer hydrogel group exhibited new translucent cartilage and repaired subchondral bone, indicating that the hydrogel can enhance the repair of osteochondral defects. | Jinfeng Liao Taoran Tian Sirong Shi Xueping Xie Quanquan Ma Guo Li Yunfeng Lin | 2017 | Bone Research2017，5，2： | 10 |
| 13 | Rice St#pe Virus Interferes with S-acylation of Remorin and Induces Its Autophagic Degradation to Facilitate Virus Infection显示文摘 | Shuai Fu Yi Xu Chenyang Li Yi Li dianxiang Wu Xueping Zhou | 2018 | Molecular Plant2018，11，2： | 10 |
| 14 | Complete chloroplast genome sequences of Schisandra chinensis:genome structure,comparative analysis,and phylogenetic relationship of basal angiosperms显示文摘Dear Editor,Schisandra chinensis(Turcz.)Baill.belongs to family Schisandraceae.Its fruit called'Wu Wei Zi'in Chinese is a well-known medicinal material,which is used to treat chronic cough and dyspnea,nocturnal emission,enuresis,etc.(National Pharmacopoeia Committee,2015).Except for S.chinensis,many species of Schisandraceae,such as S.sphenanthera,S.lancifolia and S.rubriflora,are used as the original plants of folk medicines.Although these species have similar traditional effects,modern | Haojie Guo Jiushi Liul Li Luo Xueping Wei Jin Zhang Yaodong Qi Bengang Zhang Haitao Liu Peigen Xiao | 2017 | Science China(Life Sciences)2017，60，11： | 9 |
| 15 | Variable sets method for urban flood vulnerability assessment显示文摘On the basis of dialectics basic laws and mathematical theorems of variable sets,this paper proposes a variable sets method for urban flood vulnerability assessment.In this method,the comprehensive relative membership degree of multi-indices is represented by an index relative difference degree,which follows the characteristics of dialectical philosophy and mathematics.According to the quality-quantity exchange theorem,the relative difference degree of two adjacent levels(h and h+1),whose index standard interval values cross the boundaries,equals 0 in the urban flood vulnerability assessment.On the basis of the opposite unity theorem,the sum of relative membership degrees should be equal to 1 when indices lie in the adjacent degrees h and h+1.The variable sets method is proved to be theoretically rigorous and computationally simple.This paper takes 29 cities of Hunan province as an example to assess the urban flood vulnerability,and then compares the results from this newly developed method with the assessment results obtained from the fuzzy comprehensive evaluation and fuzzy set pair analysis methods. | CHEN ShouYu XUE ZhiChun LI Min ZHU XuePing | 2013 | Science China(Technological Sciences)2013，56，12： | 9 |
| 16 | High-efficiency and multiplex adenine base editing in plants using new TadA variants显示文摘Recently reported adenine base editors(ABEs)exhibit powerful potential for targeted gene correction as well as developing gain-of-function mutants and novel germplasms for both gene function studies and crop breeding.However,editing efficiency varies significantly among different target sites.Here,we investigated the activities of three evolved E.coli adenosine deaminase TadA variants(TadA8e,TadA8.17,and TadA8.20)side-by-side in transgenic rice.We found that TadA8e outperforms TadA8.17 and TadA8.20,and induces efficient A-to-G conversion at all tested sites in the rice genome,including those that were un-editable by ABE7.10 in our previous experiments.Furthermore,V82S/Q154R mutations were incorporated into TadA8e,resulting in a new variant that we named TadA9.Our data show that TadA9 is broadly compatible with CRISPR/SpCas9,CRISPR/SpCas9-NG,and CRISPR/SpRY,as well as CRISPR/ScCas9 nickase systems,achieving comparable or enhanced editing in a larger editing window at diverse PAM sites as compared with TadA8e.Finally,TadA9 was used to simultaneously install novel SNPs in four endogenous herbicide target genes in the commercial rice cultivar Nangeng 46 for potential field application in.weed control.Collectively,we successfully generated a series of novel ABEs that can efficiently edit adenosines in the rice genome.Our findings suggest that TadA9 and TadA8e have great potentials in the development of plant base editors and crop molecular breeding. | Daqi Yan Bin Ren Lang Liu Fang Yan Shaofang Li Guirong Wang Wenxian Sun Xueping Zhou Huanbin Zhou | 2021 | Molecular Plant2021，14，5： | 8 |
| 17 | A Middle-Upper Devonian Boundary Section in the Open Platform,Platform Margin Facies of Guilin,South China显示文摘The Caiziyan Middle and Upper Devonian boundary section is located approximately 30 km northeast of Guilin.It hosts relatively abundant benthic and common-rare pelagic fossils,including brachiopods,corals,tentaculites,and conodonts,which may serve as a better suitable section for pelagic and neritic stratigraphic correlation.In this section,10'standard'conodont zones are recognized across the Givetian-Frasnian boundary,including,in descending order,the Lower hassi Zone,punctata Zone,transitans Zone,the Upper falsiovalis Zone,the Lower falsiovalis Zone,disparilis Zone,the Upper hermanni-cristatus Zone,the Lower hermanni-cristatus Zone,the Upper varcus Zone, and the Middle varcus Zone,all of which are defined by the first occurrence of their defining conodont species.The Middle-Upper Devonian(Givetian-Frasnian) boundary is defined by the first occurrence of Ancyrodella pristina in accordance with the Global Stratotype Section and Point(GSSP),which is assigned at 6.2m above the base of bed 19 in the Caiziyan section. | LI Hua MA Xueping WEI Longming | 2009 | Acta Geologica Sinica(English Edition)2009，83，3： | 7 |
| 18 | The interaction of CpEBF1 with CpMADSs is involved in cell wall degradation during papaya fruit ripening显示文摘Ethylene plays a pivotal role in climacteric fruit ripening;whereas 1-MCP,a non-toxic antagonist of ethylene,prevents ethylene-dependent responses and fruit ripening.In this study,a short-term treatment(1 h)with 400 nL L^(−1)1-MCP delayed the ripening of harvested papaya.However,long-term application of 1-MCP(400 nL L^(−1),16 h)resulted in abnormal fruit ripening,with the fruits exhibiting normal yellowing without softening,significantly higher cellulose and lignin contents,and intact cell walls(CW).Furthermore,we found that long-term treatment with 1-MCP significantly inhibited the expression of CpEBF1,an EIN3-binding F-box-1 gene.A protein interaction analysis using yeast two-hybrid,BiFC and GST pull-down assays showed that CpEBF1 interacts with the CpMADS1/3 and CpEIL1 proteins.The interaction of CpEBF1 with CpMADS1/3 further activated the activities of CW-degradation gene promoters.Subcellular localization showed that these proteins were localized in the nucleus.Additionally,the expression levels of CpMADS1/3,CpEIL1,and several CW-degradation-related genes were significantly downregulated by long-term 1-MCP treatment.Therefore,we propose that the inhibited expression of CpEBF1 and CpMADS1/3 resulted in the repressed activation of CW-degradation-related genes via their interaction,thereby resulting in fruit softening disorders. | Xiaochun Ding Xiaoyang Zhu Lanlan Ye Shuangling Xiao Zhenxian Wu Weixin Chen Xueping Li | 2019 | Horticulture Research2019，6，1： | 7 |
| 19 | Identification of a novel DNA molecule associated with Tobacco leaf curl virus显示文摘A circular DNA molecule, designated as DNAp, was identified in tobacco plants infected with Tobacco leaf curl virus (TLCV) isolates Y5 and Y8 by PCR using primers based on the conserved region of the two reported DNAp sequences of whitefly-transmitted geminiviruses (WTGs). The complete nucleotide sequences of DNAβ of Y5 and Y8 (TLCV DNAβ) were determined. Y5 DNAβ comprises 1333 nucleotides encoding 8 predicted ORFs with 4 ORFs in virion-sense DNA and 4 ORFs in complementary-sense DNA; Y8 DNAP consists of 1338 nucleotides encoding 7 predicted ORFs with 4 ORFs in virion-sense DNA and 3 ORFs in complementary-sense DNA. TLCV DNAβhas little sequence homology to DNA-A of TLCV, except that it shares conserved TAATATTAC loop sequence with TLCV DNA-A. Sequence comparison showed that Y5 DNAβ shared 85% sequence homology with Y8 DNAβ, and both Y5 DNAβ and Y8 DNAβ had relatively low sequence identity (51%-65%) with the reported DNAβ molecules associated with Agera-tum yellow vein virus and Cotton leaf | XIE Yan ZHOU Xueping LI Zhenghe ZHANG Zhongkai LI Guixin | 2002 | Chinese Science Bulletin2002，47，15： | 6 |
| 20 | Ability to detect weak effective seismic signals by utilizing chaotic vibrator system显示文摘Taking the advantage of CVS (Chaotic Vibrator System) sensitivity of large-scale periodic phase-state response to quasi-periodic or periodic signals,a series of numerical experiments were made to understand the ability of CVS to detect weak effective seismic signals in the common-shot seismic record distorted by strong stochastic noise. The re-sults demonstrate that the large-scale periodic phase-states of CVS are correlated with the signal composition of the quasi-periodic wavelet sequence constructing from horizontal moveout of seismic events,noise strength and the noise distortion de-gree to signal. For the same kind of events,the higher the noise distortion degree is,the lower the detectable SNR can be reached by CVS. For seismic data with the same noise distortion degree,the closer the scanning seismic velocity (the trial moveout ve-locity) approaches to the accurate velocity,the higher the detectable SNR can be reached by CVS. More-over,the truncating scanning velocities form an asymmetric belt,which indirectly makes CVS achieve a large-scale periodic phase-state and then the ratio of wavelet distortion coefficients in events can be a biggish variable scope. | LI Yue YANG Baojun YUAN Ye ZHAO Xueping LIN Hongbo | 2006 | Chinese Science Bulletin2006，51，24： | 6 |
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