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| 1 | De novo mutation in ATP6V1B2 impairs lysosome acidification and causes dominant deafness-onychodystrophy syndrome显示文摘 | Yongyi Yuan Jianguo Zhang Qing Chang Jin Zeng Feng Xin Jianjun Wang Qingyan Zhu Jing Wu Jingqiao Lu Weiwei Guo Xukun Yan Hui Jiang Binfei Zhou Qi Li Xue Gao Huijun Yuan Shiming Yang Dongyi Han Zixu Mao Ping Chen Xi Lin Pu Dai | 2014 | Cell Research2014，24，11： | 13 |
| 2 | Highly reactive, flexible yet green Se precursor for metal selenide nanocrystals: Se-octadecene suspension (Se-SUS)显示文摘在 octadecene (Se 怀疑) 的好硒粉末(100 或 200 网孔) 的暂停证明了是一位高效的、万用的、方便的、可再现的、还绿的硒先锋。Se 怀疑的优点从它的高度反应的化学性质和灵活性产生。这二个特征使与多样的作文和结构执行高质量的金属硒化物 nanocrystals 的合成可能,包括二进制代码,核心 / 壳,做的转变金属,和复杂作文 nanocrystals。这些成功进一步证明 Se 怀疑是为在高质量的硒化物 nanocrystals 的合成解决一些长期的挑战的一位强大的 Se 先锋。例如,Se怀疑成功地作为一位 Se 先锋被采用因为在高质量的核心/壳 nanocrystals 轰炸生长代替昂贵、高度有毒的先锋,例如Se磷化氢和 bis-trimethylsilyl 硒化物,与极大地降低的取向附生的温度(象 150 一样低???????????????? | Chaodan Pu Jianhai Zhou Runchen Lai Yuan Niu Wennuan Nan Xiaogang Peng | 2013 | Nano Research2013，6，9： | 12 |
| 3 | GJB2 mutation spectrum in deaf population in a typical southeastern area of China显示文摘Mutations in GJB2 gene are the most frequently found mutations in patients with nonsyndromic hearing impairment. However, the spectrum and prevalence of mutations in this gene vary among different ethnic groups. In China, 30,000 infants are born with congenital hearing impairment annually. In order to provide appropriate genetic testing and counseling to the families, we investigated the molecular etiology of nonsyndromic deafness in 103 unrelated school children attending Nantong School for the Deaf and Mute in Jiangsu Province, China. The coding exon of the GJB2 gene was PCR amplified and sequenced. Sixty two GJB2 mutant alleles were identified in 35.9% (37/103) of the patients. Twenty five patients carried two pathogenic mutations and 12 patients carried one mutant allele. The 235delC was the most common mutation accounting for 69.4% (43/62) of GJB2 mutant alleles. The GJB2 mutant alleles accounted for 30.1% (62/206) of all chromosomes responsible for nonsyndromic hearing impairment. Testing of the 3 most prevalent deleterious frame shift mutations in this cohort detected 100% of all GJB2 mutant alleles. These results demonstrate that an effective genetic testing of GJB2 gene for patients and families with nonsyndromic hearing impairment is possible. | DAI Pu1*, YOU Yi-wen2*, CUI Jing-hong2*, YU Fei1, HAN Bing 1, KANG Dong-yang1, YUAN Hui-jun1, HAN Dong-yi1, 1. Department of Otolaryngology, PLA General Hospital, Beijing, People’s Republic China, 100853 2. Department of Otolaryngology, Nantong University Affiliated Hospital, Nantong, Jiangsu Province, People’s Republic China, 226001 *Pu Dai, Yiwen You, Jinghong Cui contribute equally to this paper | 2006 | Journal of Otology2006，1，2： | 10 |
| 4 | Direct differentiation of atrial and ventricular myocytes from human embryonic stem cells by alternating retinoid signals显示文摘尽管 myocyte 房间移植研究为心肌的梗塞建议了一个有希望的治疗学的潜力,到为心肌的修理的临床的治疗的发展的一个主要障碍是与为移植获得相对同类的室的 myocytes 联系的困难。人的胚胎的干细胞(hESCs ) 是 cardiomyocytes 的有希望的来源。这里,我们报导发信号的 retinoid 在 hESCs 的心脏的区别期间调整 atrial 对室的 myocytes 的命运说明。我们发现少量和 pan-retinoic 酸受体对手 BMS-189453 (RAi ) 显著地增加了 hESCs 的心脏的区别效率。为了调查 retinoid,工作,我们把对待 Noggin+RAi 的文化与对待 Noggin+RA 的文化作比较。我们的结果证明室特定的基因 IRX-4 的表示层次极端地在对待 Noggin+RAi 的文化被提高。MLC-2V,另一个室特定的标记,在对待 Noggin+RAi 的文化,然而并非在对待 Noggin+RA 的文化的 cardiomyocytes 在 cardiomyocytes 的多数被表示。流动 cytometry 分析和 electrophysiological 研究与 64.7 | Qiangzhe Zhang Junjie Jiang Pengcheng Han Qi Yuan Jing Zhang Xiaoqian Zhang Yanyan Xu Henghua Cao Qingzhang Meng Li Chen Tian Tian Xin Wang Pu Li Jurgen Hescheler Guangju Ji Yue Ma | 2011 | Cell Research2011，21，4： | 10 |
| 5 | Identification of a novel mutation in POU3F4 for prenatal diagnosis in a Chinese family with X-linked nonsyndromic hearing loss显示文摘We present the clinical and genetic findings for a Chinese family with X-linked non-syndromic hearing loss in which the affected males showed congenital profound sensorineural hearing impairment. In two affected brothers, the computer tomography of temporal bone showed bilateral dilation of the internal auditory canal with fistulous communication between the lateral canal and the basal cochlear turn, which is consistent with the typical DFNX2 phenotype. A missense mutation (c.647G→A) in the POU3F4 gene caused a substitu- tion from glycine to glutamic acid at position 216 (p.G216E), and this mutation was found to consistently cosegregate with the deafness phenotype in the family. The mutation resulted in the loss of function of the POU3F4 by decreasing the affinity between the protein and DNA, as shown in silico by the structural analysis. Prenatal diagnosis of pregnant proband of this family revealed the c.647G→A muta- tion in DNA extracted from the amniotic fluid surrounding the fetus. The appropriate use of genetic testing and prenatal diagnosis plays a key role in reducing the recurrence of genetic defects in high-risk families. | Jianzhong Li Jing Cheng Yanping Lu Yu Lu Airing Chen YiSun Dongyang Kang Xin Zhang Pu Dai Dongyi Han Huijun Yuan | 2010 | Journal of Genetics and Genomics2010，37，12： | 9 |
| 6 | A two-step lineage reprogramming strategy to generate functionally competent human hepatocytes from fibroblasts显示文摘Terminally differentiated cells can be generated by lineage reprogramming,which is,however,hindered by incomplete conversion with residual initial cell identity and partial functionality. Here, we demonstrate a new reprogramming strategy by mimicking the natural regeneration route, which permits generating expandable hepatic progenitor cells and functionally competent human hepatocytes. Fibroblasts were first induced into human hepatic progenitor-like cells (hHPLCs), which could robustly expand in vitro and efficiently engraft in vivo. Moreover, hHPLCs could be efficiently induced into mature human hepatocytes (hiHeps) in vitro, whose molecular identity highly resembles primary human hepatocytes (PHHs). Most importantly, hiHeps could be generated in large quantity and were functionally competent to replace PHHs for drug-metabolism estimation, toxicity prediction and hepatitis B virus infection modeling. Our results highlight the advantages of the progenitor stage for successful lineage reprogramming. This strategy is promising for generating other mature human cell types by lineage reprogramming. | Bingqing Xie Da Sun Yuanyuan Du Jun Jia Shicheng Sun Jun Xu Yifang Liu Chengang Xiang Sitong Chen Huangfan Xie Qiming Wang Guangya Li Xuehui LYU Hui Shen Shiyu Li Min Wu Xiaonan Zhang Yue Pu Kuanhui Xiang Weifeng Lai Peng Du Zhenghong Yuan Cheng Li Yan Shi Shichun Lu Hongkui Deng | 2019 | Cell Research2019，29，9： | 9 |
| 7 | Identifying the Sources of Solutes in Karst Groundwater in Chongqing,China:a Combined Sulfate and Strontium Isotope Approach显示文摘从石灰岩地区常见的地形的地下水地下的溪流在世界喝水供应,和石灰岩地区常见的地形水的 hydrochemical 特征的很重要的来源之中被自然环境和人影响。因此, hydrochemistry 的学习和它的溶质是来源是很重要的保证生活支持系统的正常函数。在这份报纸, thirtyfive 代表石灰岩地区常见的地形地下水样品从不同含水土层(石灰石和白云石) 被收集,各种各样的陆地使用录入重庆跟踪溶质和相对 hydrochemical 过程的来源。在重庆的石灰岩地区常见的地形地下水的 Hydrogeochemical 类型主要具有 CaHCO3 类型或 Ca (Mg ) HCO3 类型。然而,石灰岩地区常见的地形地下水的一些 hydrochemical 类型是 K+Na+CaSO4 类型(G25 地点) 或 CaHCO3+SO4 类型(G26 和 G14 地点) ,显示这些地点的 hydrochemistry 可能被人为的活动或唯一的地质的特征强烈影响。学习地下水的溶解 Sr 集中从 0.57 ~ 15.06 mmol/L ,并且 87Sr/86Sr 从 0.70751 ~ 0.71627 变化了。34SSO42 掉进 6.821.5 的一个范围,与 5.6 的吝啬的价值。87Sr/86Sr 的变化和地下水样品的 Sr 价值显示 Sr 元素被捱过石灰石,白云石和硅酸盐岩石控制。然而, 87Sr/86Sr 对 Sr2+/ 的数字[K++Na+] 证明人为的输入显然也贡献了 Sr 内容。为跟踪详细人为的效果,我们跟踪了石灰岩地区常见的地形地下水根据潜在的硫酸盐来源的 34S 价值在重庆取样的收集的溶质的来源。34S 的变化和地下水样品的 1/SO42 价值显示大气的酸免职(AAD ) ,石膏(GD ) 的溶解,硫化物矿物质(OS ) 的氧化或人为的输入(SF:污水或化肥) 在石灰岩地区常见的地形地下水贡献了溶质。到在重庆石灰岩地区常见的地形区域的地下水的硫化物矿物质,大气的酸沉积物和人为的输入的氧化的影响是普遍的。 | PU Junbing YUAN Daoxian ZHANG Cheng ZHAO Heping | 2012 | Acta Geologica Sinica(English Edition)2012，86，4： | 9 |
| 8 | Facile and Scalable Preparation of Fluorescent Carbon Dots for Multifunctional Applications显示文摘 | Dan Wang Zhiyong Wang Qiuqiang Zhan Yuan Pu Jie-xin wang Neil R. Foster Liming Dai | 2017 | Engineering2017，3，3： | 9 |
| 9 | Association of Overlapped and Un-overlapped Comorbidities with COVID-19 Severity and Treatment Outcomes: A Retrospective Cohort Study from Nine Provinces in China显示文摘Objective Several COVID-19 patients have overlapping comorbidities. The independent role of each component contributing to the risk of COVID-19 is unknown, and how some non-cardiometabolic comorbidities affect the risk of COVID-19 remains unclear.Methods A retrospective follow-up design was adopted. A total of 1,160 laboratory-confirmed patients were enrolled from nine provinces in China. Data on comorbidities were obtained from the patients’ medical records. Multivariable logistic regression models were used to estimate the odds ratio(OR) and 95% confidence interval(95% CI) of the associations between comorbidities(cardiometabolic or non-cardiometabolic diseases), clinical severity, and treatment outcomes of COVID-19.Results Overall, 158(13.6%) patients were diagnosed with severe illness and 32(2.7%) had unfavorable outcomes. Hypertension(2.87, 1.30–6.32), type 2 diabetes(T2 DM)(3.57, 2.32–5.49),cardiovascular disease(CVD)(3.78, 1.81–7.89), fatty liver disease(7.53, 1.96–28.96), hyperlipidemia(2.15, 1.26–3.67), other lung diseases(6.00, 3.01–11.96), and electrolyte imbalance(10.40, 3.00–26.10)were independently linked to increased odds of being severely ill. T2 DM(6.07, 2.89–12.75), CVD(8.47,6.03–11.89), and electrolyte imbalance(19.44, 11.47–32.96) were also strong predictors of unfavorable outcomes. Women with comorbidities were more likely to have severe disease on admission(5.46,3.25–9.19), while men with comorbidities were more likely to have unfavorable treatment outcomes(6.58, 1.46–29.64) within two weeks.Conclusion Besides hypertension, diabetes, and CVD, fatty liver disease, hyperlipidemia, other lung diseases, and electrolyte imbalance were independent risk factors for COVID-19 severity and poor treatment outcome. Women with comorbidities were more likely to have severe disease, while men with comorbidities were more likely to have unfavorable treatment outcomes. | MA Yan ZHU Dong Shan CHEN Ren Bo SHI Nan Nan LIU Si Hong FAN Yi Pin WU Gui Hui YANG Pu Ye BAI Jiang Feng CHEN Hong CHEN Li Ying FENG Qiao GUO Tuan Mao HOU Yong HU Gui Fen HU Xiao Mei HU Yun Hong HUANG Jin HUANG Qiu Hua HUANG Shao Zhen JI Liang JIN Hai Hao LEI Xiao LI Chun Yan LI Min Qing LI Qun Tang LI Xian Yong LIU Hong De LIU Jin Ping LIU Zhang MA Yu Ting MAO Ya MO Liu Fen NA Hui WANG Jing Wei SONG Fang Li SUN Sheng WANG Dong Ting WANG Ming Xuan WANG Xiao Yan WANG Yin Zhen WANG Yu Dong WU Wei WU Lan Ping XIAO Yan Hua XIE Hai Jun XU Hong Ming XU Shou Fang XUE Rui Xia YANG Chun YANG Kai Jun YUAN Sheng Li ZHANG Gong Qi ZHANG Jin Bo ZHANG Lin Song ZHAO Shu Sen ZHAO Wan Ying ZHENG Kai ZHOU Ying Chun ZHU Jun Teng ZHU Tian Qing ZHANG Hua Min WANG Yan Ping WANG Yong Yan 无 | 2020 | Biomedical and Environmental Sciences2020，33，12： | 8 |
| 10 | Sirt1 inhibits renal tubular cell epithelial–mesenchymal transition through YY1 deacetylation in diabetic nephropathy显示文摘Silent information regulator 1(Sirt1)is a deacetylase,which plays an important role in the occurrence and development of diabetic nephropathy(DN).Our previous study shows that Yin yang 1(YY1),a widely expressed zinc finger DNA/RNA-binding transcription factor,is a novel regulator of renal fibrosis in diabetic nephropathy.Since the activity of YY1 is regulated via acetylation and deacetylation modification,this study aimed to explore whether Sirt1-induced deacetylation of YY1 mediated high glucose(HG)-induced renal tubular epithelial–mesenchymal transition(EMT)and renal fibrosis in vivo and in vitro.We first confirmed that Sirt1 expression level was significantly decreased in the kidney of db/db mice and in HG-treated HK-2 cells.Diabetes-induced Sirt1 reduction enhanced the level of YY1 acetylation and renal tubular EMT.Then,we manipulated Sirt1 expression in vivo and in vitro by injecting resveratrol(50 mg·kg^(−1)·d^(−1).ip)to db/db mice for 2 weeks or application of SRT1720(2.5μM)in HG-treated HK-2 cells,we found that activation of Sirt1 reversed the renal tubular EMT and YY1 acetylation induced by HG condition.On the contrary,Sirt1 was knocked down in db/m mice or EX527(1μM)was added in HK-2 cells,we found that inhibition of Sirt1 exacerbated renal fibrosis in diabetic mice and enhanced level of YY1 acetylation in HK-2 cells.Furthermore,knockdown of YY1 inhibited the ameliorating effect of resveratrol on renal tubular EMT and renal fibrosis in db/db mice.In conclusion,this study demonstrates that Sirt1 plays an important role in renal tubular EMT of DN through mediating deacetylation of YY1. | Lei Du Xuan Qian Yuan Li Xi-zhi Li Lin-lin He Liu Xu Yi-qi Liu Cheng-cheng Li Pu Ma Fang-lin Shu Qian Lu Xiao-xing Yin | 2021 | Acta Pharmacologica Sinica2021，42，2： | 8 |
| 11 | Nutrition deficiency promotes apoptosis of cartilage endplate stem cells in a caspase-independent manner partially through upregulating BNIP3显示文摘营养缺乏被报导在兔子导致 chondrocytes 的 apoptosis 和软骨终板(CEP ) 的退化。软骨终板干细胞(CESC ) 为结构的完整和 CEP 的功能是重要的。(BNIP3 ) Bcl-2/adenovirus E1B 19-kDa-interacting 蛋白质 3 被报导了调整 apoptosis, autophagy,和 cytoprotection。在这研究,我们试图决定营养缺乏是否导致 CESC 的 apoptosis,并且 BNIP3 相关的小径是否在营养缺乏期间在 CESC 被激活。从堕落人的 CEP 孤立的 CESC 在正常或营养缺乏的状况下面是有教养的。然后, apoptosis 被流动 cytometry 分析。表示和 BNIP3 的细胞内部的本地化被量的即时聚合酶链反应,西方的污点分析,和 immunofluorescence 试金分别地检测。Mitochondrial 膜潜力(MMP ) 和 caspase-3 活动被 JC-1 染色和 caspase-3 活动试金测量。我们的结果证明那营养缺乏在 CESC 支持 apoptosis 和 BNIP3 表示。尤其是, BNIP3 击倒能部分减少营养 CESC 的导致缺乏的 apoptosis。另外,营养缺乏能也导致 BNIP3 的 upregulation,导致 BNIP3 的 mitochondrial translocation 和在以一种时间依赖者方式的 CESC 的 MMP 的损失。然而,营养缺乏没在 CESC 在 caspase-3 活动显示出效果。在摘要,营养缺乏可以通过 upregulating BNIP3 部分支持 CESC apoptosis,它可能以一种 caspase 独立的方式导致 BNIP3 相关的小径的激活和 CESC 的 apoptosis。 | Zhiliang He Luqiao Pu Chao Yuan Min Jia Jian Wang | 2017 | Acta Biochimica et Biophysica Sinica2017，49，1： | 7 |
| 12 | Protonation induced high-T_c phases in iron-based superconductors evidenced by NMR and magnetization measurements显示文摘Chemical substitution during growth is a well-established method to manipulate electronic states of quantum materials, and leads to rich spectra of phase diagrams in cuprate and iron-based superconductors. Here we report a novel and generic strategy to achieve nonvolatile electron doping in series of(i.e.11 and 122 structures) Fe-based superconductors by ionic liquid gating induced protonation at room temperature. Accumulation of protons in bulk compounds induces superconductivity in the parent compounds, and enhances the Tclargely in some superconducting ones. Furthermore, the existence of proton in the lattice enables the first proton nuclear magnetic resonance(NMR) study to probe directly superconductivity. Using Fe S as a model system, our NMR study reveals an emergent high-Tcphase with no coherence peak which is hard to measure by NMR with other isotopes. This novel electric-fieldinduced proton evolution opens up an avenue for manipulation of competing electronic states(e.g.Mott insulators), and may provide an innovative way for a broad perspective of NMR measurements with greatly enhanced detecting resolution. | Yi Cui Gehui Zhang Haobo Li Hai Lin Xiyu Zhu Hai-Hu Wen Guoqing Wang Jinzhao Sun Mingwei Ma Yuan Li Dongliang Gong Tao Xie Yanhong Gu Shiliang Lie Huiqian Luo Pu Yu Weiqiang Yu | 2018 | Science Bulletin2018，63，1： | 7 |
| 13 | Mitochondrial DNA A1555G mutation screening using a testing kit method and its significance in preventing aminoglycoside-related hearing loss显示文摘To report a new screening method for mitochondrial DNA 1555A→G mutation and the results of genotype analysis in 19 maternal inherited deafness pedigrees. Method Five hundred and forty-six non-syndromic neuro-sensory hearing loss patients were tested for 1555A→G mutation using a new compact testing kit, which allows clear distinction between wild type and 1555 A→G mutated mtDNAs. Results Nineteen subjects among the 546 patients (3.48%) were found to carry mtDNA A1555G mutation. The results were confirmed by sequencing in an ABI 3100 Avant sequencer. Conclusions Maternal inherited deafness families are a frequently seen in outpatient group. The detection of mtDNA 1555 A→G mutation with a low cost, ready to use detection kit is needed and suitable in China for large scale screening and preventive testing before usage of aminoglycoside antibiotics. | LIU Xin,1 DAI Pu,1* HUANG Deliang,1 YUAN Huijun,1 LI Weiming,1 YU Fei,1 ZHANG Xin,1 KANG Dongyang,1 CAO Juyang,1 YANG Weiyan,1 HAN Dongyi,1 JIN Zhengce2, GUAN Minxin3 1. Department of Otolaryngology, Chinese PLA General Hospital, Beijing, China2. Weihai Aomaier Gene Technological CO.,LTD.,Weihai,Shandong 264200, China.3. Division and Program in Human Genetics and Center for Hearing and Deafness Research, Cincinnati Children’s Hospital Medical Center, Cincinnati, Ohio, USA | 2006 | Journal of Otology2006，1，1： | 7 |
| 14 | MiRNA-365 and miRNA-520c-3p respond to risperidone treatment in first-episode schizophrenia after a 1 year remission显示文摘 | LIUSha YUAN Yan-bo GUAN Li-li WEI Hui CHENG Zhang HAN Xue YANG Lei PU Cheng-cheng YANG Fu-de LU zheng DENG Hong ZHAO Jing-ping YUXin | 2013 | Chinese Medical Journal2013，，14： | 7 |
| 15 | Influencing factors of the neurodevelopment of high-risk infants显示文摘Background High-risk infants refer to newborns exposed to high-risk factors in the prenatal, natal or postnatal period. High-risk infants are at high risk of developmental retardation, and early identification of developmental abnormalities plays a vital role in improving high-risk infants' quality of life.Aims To describe the neurodevelopment of high-risk infants aged less than 1 year old, and to analyse the incidences and influencing factors of neurodevelopmental abnormalities in order to provide a basis for neurodevelopment monitoring and management of highrisk infants.Methods High-risk infants born between January 2016 and December 2016 in the maternity and infant health hospitals of three districts in Shanghai were followed up.The Gesell Developmental Scale was used to assess the neurodevelopmental level at the time of recruitment(0-2 months) and at 9 months. Univariate and multivariate analyses of the influencing factors were conducted.Results 484 high-risk infants(male 51 %, female 49%)with an average gestation age of 36.5±2.2 weeks were recruited. At the time of recruitment, the average age was2.1(0.8) months, and the developmental quotient(DQ)scores of full-term high-risk infants in motor(t=3.542,p=0.001), cognitive(t=3.125, p=0.002), language(t=3.189, p=0.002) and social(t=3.316, p=0.001) areas were higher than those of preterm infants. The incidences of developmental abnormalities of full-term high-risk infants in motor(χ~2 =9.452, p=0.002), cognitive(χ~2=6.258, p=0.012), language(χ~2 =12.319, p =0.001) and social(χ~2 =6.811, p=0.009) areas were lower than the preterm infants. At 9 months, there was no difference in the DQ scores and incidences of developmental abnormalities in four areas between full-term and preterm high-risk infants, and the incidence of developmental abnormalities was around 10%.Conclusion The incidence of neurodevelopmental abnormalities in high-risk infants aged less than 1 year old is high. Preterm birth and parental bad habits are significant factors affecting the neurodevelopment.Monitoring and early interventions help to improve highrisk infants' neurodevelopment. | Yuan Tian Chuncao Zhang Guangjun Yu Xiangying Hu Zheng Pu Liyu Ma | 2018 | General Psychiatry2018，31，6： | 6 |
| 16 | Analysis of diferentially expressed protein from primary and recurrent ovarian cancer serum显示文摘Objective:To study the value of the differentially expressed proteins from primary and recurrent ovarian cancer serum for early diagnosis of primary and recurrent ovarian cancer.Methods: WCX kit(Bruker Daltonics GraBH) and matrix-assisted laser desorption/ionization time-offlight mass spectrometry(MALDI-TOF-MS]technology were used to detect serum samples from 49 patients with primary ovarian cancer and 21 patients with recurrent disease.Results:In the mass range(Mr) from 1 000 to 12 000 Da,eight differentially expressed protein peaks were screened from primary ovarian cancer serum.Among them,four protein peaks with Mr 1 457,1 857,2 202, 7 761 were lowly expressed and the others with Mr 2 946,5 333,5 859,5 901 were highly expressed. Ten differentially expressed protein peaks were screened from recurrent ovarian cancer serum. Among them.1 944,1 980,2 080,2 661,2 993,4 450,4 659,5 359 Da protein expressions were increased significantly,and 1897,7868 Da protein expressions were decreased significantly.The pattern of primary ovarian cancer was applied to 8 early-stage ovarian cancer serum samples, and 7 serum samples were successfully predicted with the accuracy of 87.5%.The pattern of recurrent ovarian cancer was applied to 9 without pelvic:or abdominal mass recurrent ovarian cancer serum samples,and 8 serum samples were successfully predicled with the accuracy of 88.9%.Conclusions:Combination of MALDI-TOF-MS and WCX kit technology can directly screen the diferrential expressed protein from primary and recurrent ovarian cancer serum.They have clinical significance for enhancement of sensitivity and specificity of ovarian cancer diagnosis. | Yuan Wang Jin-Jin Yu Ting Zhu Ling Xu Ming Xu Yu-Zheng Huang Hong Pu Chun-Qing Yu | 2012 | Asian Pacific Journal of Tropical Medicine2012，5，7： | 5 |
| 17 | Effect of HIV-1 Tat on Secretion of TNF-α and IL-1β by U87 Cells in AIDS Patients with or without AIDS Dementia Complex显示文摘Objective To explore the role of HIV-1 tat gene variations in AIDS dementia complex(ADC) pathogenesis.Methods HIV-1 tat genes derived from peripheral spleen and central basal ganglia of an AIDS patient with ADC and an AIDS patient without ADC were cloned for sequence analysis. HIV-1 tat gene sequence alignment was performed by using CLUSTAL W and the phylogentic analysis was conducted by using Neighbor-joining with MEGA4 software. All tat genes were used to construct recombinant retroviral expressing vector MSCV-IRES-GFP/tat. The MSCV-IRES-GFP/tat was cotransfected into 293T cells with pCMV-VSV-G and pUMVC vectors to assemble the recombinant retrovirus. After infection of gliomas U87 cells with equal amount of the recombinant retrovirus, TNF-α, and IL-1β concentrations in the supernatant of U87 cells were determined with ELISA. Results HIV-1 tat genes derived from peripheral spleen and central basal ganglia of the AIDS patient with ADC and the other one without ADC exhibited genetic variations. Tat variations and amino acid mutation sites existed mainly at Tat protein core functional area(38-47aa). All Tat proteins could induce U87 cells to produce TNF-α and IL-1β, but the level of IL-1β production was different among Tat proteins derived from the ADC patient's spleen, basal ganglia, and the non-ADC patient's spleen. The level of Tat proteins derived from the ADC patient's spleen, basal ganglia, and the non-ADC patient's spleen were obviously higher than that from the non-ADC patient's basal ganglia. Conclusion Tat protein core functional area(38-47aa) may serve as the key area of enhancing the secretion of IL-1β. This may be related with the neurotoxicity of HIV-1 Tat. | ZHAO Li PU Shuang Shuang GAO Wen Hua CHI Yuan Yuan WEN Hong Ling WANG Zhi Yu SONG Yan Yan YU Xue Jie | 2014 | Biomedical and Environmental Sciences2014，27，2： | 5 |
| 18 | High glucose levels impact visual response properties of retinal ganglion cells in C57 mice—An in vitro physiological study显示文摘This study investigated visual response properties of retinal ganglion cells(RGCs) under high glucose levels. Extracellular single-unit responses of RGCs from mouse retinas were recorded. And the eyecup was prepared as a flat mount in a recording chamber and superfused with Ames medium. The averaged RF size of the ON RGCs(34.1±2.9, n=14) was significantly smaller than the OFF RGCs under the HG(49.3±0.3, n=12)(P<0.0001) conditions. The same reduction pattern was also observed in the osmotic control group(HM) between ON and OFF RGCs(P<0.0001). The averaged luminance threshold(LT) of ON RGCs increased significantly under HG or HM(HG: P<0.0001; HM: P<0.0002). OFF RGCs exhibited a similar response pattern under the same conditions(HG: P<0.01; HM: P<0.0002). The averaged contrast gain of ON cells was significantly lower than that of OFF cells with the HM treatment(P<0.015, unpaired Student's t test). The averaged contrast gain of ON cells was significantly higher than OFF cells with the HG treatment(P<0.0001). The present results suggest that HG reduced receptive field center size, suppressed luminance threshold, and attenuated contrast gain of RGCs. The impact of HG on ON and OFF RGCs may be mediated via different mechanisms. | Yuan Zhou Chunxia Xiao Mingliang Pu | 2017 | Science China(Life Sciences)2017，60，12： | 5 |
| 19 | The desumoylating enzyme sentrin-specific protease 3 contributes to myocardial ischemia reperfusion injury显示文摘Sentrin-specific protease 3(SENP3), a member of the desumoylating enzyme family, is known as a redox sensor and could regulate multiple cellular signaling pathways. However, its implication in myocardial ischemia reperfusion(MIR) injury is unclear. Here, we observed that SENP3 was expressed and upregulated in the mouse heart depending on reactive oxygen species(ROS) production in response to MIR injury. By utilizing si RNA-mediated cardiac specific gene silencing, SENP3 knockdown was demonstrated to significantly reduce MIR-induced infarct size and improve cardiac function. Mechanistic studies indicated that SENP3 silencing ameliorated myocardial apoptosis mainly via suppression of endoplasmic reticulum(ER) stress and mitochondrial-mediated apoptosis pathways. By contrast, adenovirusmediated cardiac SENP3 overexpression significantly exaggerated MIR injury. Further molecular analysis revealed that SENP3 promoted mitochondrial translocation of dynamin-related protein 1(Drp1) in reperfused myocardium. In addition, mitochondrial division inhibitor-1(Mdivi-1), a pharmacological inhibitor of Drp1, significantly attenuated the exaggerated mitochondrial abnormality and cardiac injury by SENP3 overexpression after MIR injury. Taken together, we provide the first direct evidence that SENP3 upregulation pivotally contributes to MIR injury in a Drp1-dependent manner, and suggest that SENP3 suppression may hold therapeutic promise for constraining MIR injury. | Lingchen Gao Yichao Zhao Jie He Yang Yan Longwei Xu Nan Lin Qingqi Ji Renyang Tong Yanan Fu Yu Gao Yuanyuan Su Ancai Yuan Ben He Jun Pu | 2018 | Journal of Genetics and Genomics2018，45，3： | 5 |
| 20 | An Integrated Approach to Hypersonic Entry Attitude Control显示文摘This paper presents an integrated approach based on dynamic inversion(DI)and active disturbance rejection control(ADRC)to the entry attitude control of a generic hypersonic vehicle(GHV).DI is frstly used to cancel the nonlinearities of the GHV entry model to construct a basic attitude controller.To enhance the control performance and system robustness to inevitable disturbances,ADRC techniques,including the arranged transient process(ATP),nonlinear feedback(NF),and most importantly the extended state observer(ESO),are integrated with the basic DI controller.As one primary task,the stability and estimation error of the second-order nonlinear ESO are analyzed from a brand new perspective:the nonlinear ESO is treated as a specifc form of forced Li′enard system.Abundant qualitative properties of the Li′enard system are utilized to yield comprehensive theorems on nonlinear ESO solution behaviors,such as the boundedness,convergence,and existence of periodic solutions.Phase portraits of ESO estimation error dynamics are given to validate our analysis.At last,three groups of simulations,including comparative simulations with modeling errors,Monte Carlo runs with parametric uncertainties,and a six degrees-of-freedom reference entry trajectory tracking are executed,which demonstrate the superiority of the proposed integrated controller over the basic DI controller. | Zhi-Qiang Pu Ru-Yi Yuan Xiang-Min Tan Jian-Qiang Yi | 2014 | International Journal of Automation and computing2014，11，1： | 5 |
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