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| 1 | High glucose levels impact visual response properties of retinal ganglion cells in C57 mice—An in vitro physiological study显示文摘This study investigated visual response properties of retinal ganglion cells(RGCs) under high glucose levels. Extracellular single-unit responses of RGCs from mouse retinas were recorded. And the eyecup was prepared as a flat mount in a recording chamber and superfused with Ames medium. The averaged RF size of the ON RGCs(34.1±2.9, n=14) was significantly smaller than the OFF RGCs under the HG(49.3±0.3, n=12)(P<0.0001) conditions. The same reduction pattern was also observed in the osmotic control group(HM) between ON and OFF RGCs(P<0.0001). The averaged luminance threshold(LT) of ON RGCs increased significantly under HG or HM(HG: P<0.0001; HM: P<0.0002). OFF RGCs exhibited a similar response pattern under the same conditions(HG: P<0.01; HM: P<0.0002). The averaged contrast gain of ON cells was significantly lower than that of OFF cells with the HM treatment(P<0.015, unpaired Student's t test). The averaged contrast gain of ON cells was significantly higher than OFF cells with the HG treatment(P<0.0001). The present results suggest that HG reduced receptive field center size, suppressed luminance threshold, and attenuated contrast gain of RGCs. The impact of HG on ON and OFF RGCs may be mediated via different mechanisms. | Yuan Zhou Chunxia Xiao Mingliang Pu | 2017 | Science China(Life Sciences)2017，60，12： | 5 |
| 2 | Melanopsin-expressing retinal ganglion cell loss and behavioral analysis in the Thy1-CFP-DBA/2J mouse model of glaucoma显示文摘In this study,the role of melanopsin-expressing retinal ganglion cells(mRGCs) in the glaucoma-induced depressive behavioral response pattern was investigated.The CFP-D2 transgenic glaucoma animal model from five age groups was used in this study.Immunohistochemical labeling,quantitative analysis of mRGC morphology,open field test(OFT),and statistical analysis were used.In comparison with C57 BL/6 mice,the age-matched CFP-D2 mice had significantly elevated intraocular pressure(IOP).We observed parallel morphological changes in the retina,including a reduction in the density of cyan fluorescent protein(CFP) expressing cells(cells mm 2 at 2 months of age,1309±26;14 months,878±30,P<0.001),mRGCs(2 months,48±3;14 months,19±4,P<0.001),Brn3b-expressing RGCs(2 months,1283±80;14 months,950±31,P<0.001),Brn-3b expressing mRGCs(5 months,50.17%±5.5%;14 months,12.61%±3.8%,P<0.001),and reduction in the dendritic field size of mRGCs(mm2 at 2 months,0.077±0.015;14 months,0.065±0.015,P<0.05).CFP-D2 mice had hyperactive locomotor activity patterns based on OFT findings of the total distance traveled,number of entries into the center,and time spent in the center of the testing apparatus.The glaucoma induced hyperactive response pattern could be associated with dysfunctional mRGCs,most likely Brn-3b-positive mRGCs in CFP-D2 mice. | ZHANG Qi VUONG Helen HUANG Xin WANG YanLing BRECHA Nicholas C. PU MingLiang GAO Jie | 2013 | Science China(Life Sciences)2013，56，8： | 4 |
| 3 | Morphological evaluation of retinal ganglion cells expressing the L132C/T159C ChR2 mutant transgene in young adult cynomolgus monkeys显示文摘To characterize recombinant AAV2(rAAV2)-mediated expression of L132 C/T159 C ChR2 mutant in retinal ganglion cells(RGCs)of young adult cynomolgus monkeys, rAAV2 vectors carrying a fusion construct of the ChR2 mutant and GFP(ChR2-GFP) were delivered to the vitreous chamber by intravitreal injection. Expression patterns of the ChR2 mutant in RGCs were examined by immunohistochemical methods three months after injection. The RNA-binding protein with multiple splicing(RBPMS) was used as an RGC specific marker to differentiate RGCs from other retinal neurons and non-neuronal cells. The numbers of RBPMS+and GFP+ double-labeled RGCs in the central foveal varied with the eccentricity. The expression peaked within 100 μm from the edge of the foveola and drastically decreased to a single superficial RGC layer approximately 300 μm from the edge. On average,the ratio of the double-labeled RGCs versus RBPMS+ RGCs approached 0.32±0.15(n=14 fields) at the central foveal region(0.1 to 0.53 mm). We observed that the ratio reached 0.78±0.16(n=21 fields) at peripheral retinal locations(eccentricity >7 mm). This investigation demonstrates that RBPMS could serve as a valuable RGC specific marker for future investigations in this field. | Wenyao Wang Yan Nan Zhuo-Hua Pan Mingliang Pu | 2017 | Science China(Life Sciences)2017，60，11： | 2 |
| 4 | Morphological properties of medial amygdala-projecting retinal ganglion cells in the Mongolian gerbil显示文摘The amygdala is a limbic structure that is involved in many brain functions, including emotion, learning and memory. It has been reported that melanopsin-expressing retinal ganglion cells(ip RGCs) innervate the medial amygdala(Me A). However, whether conventional RGCs(c RGCs) project to the Me A remains unknown. The goal of this study was to determine if c RGCs project to the Me A and to determine the morphological properties of Me A-projecting RGCs(Me A-RGCs). Retrogradely labeled RGCs in whole-mount retinas were intracellularly injected to reveal their dendritic morphologies. Immunohistochemical staining was performed to selectively label ip RGCs(Me A-ip RGCs) and c RGCs(Me A-c RGCs). The results showed that 95.7% of the retrogradely labeled cells were c RGCs and that the rest were ip RGCs. Specifically, Me A-c RGCs consist of two morphological types. The majority of them exhibit small but dense dendritic fields and diffuse ramification patterns as previously reported in RG_(B2)(95%), while the rest exhibit small but sparse dendritic branching patterns resembling those of RG_(B3) cells(5%). Me Aip RGCs consist of M1 and M2 subtypes. The Me A-RGCs showed an even retinal distribution patterns. The soma and dendritic field sizes of the Me A-RGCs did not vary with eccentricity. In conclusion, the present results suggest that Me A-RGCs are structurally heterogeneous. These direct RGCs that input to the Me A could be important for regulating amygdala functions. | Liju Luan Chaoran Ren Wenyao Wang Yan Nan Jie Gao Mingliang Pu | 2018 | Science China(Life Sciences)2018，61，6： | 2 |
| 5 | New treatment for amblyopia based on rules of synaptic plasticity:a randomized clinical trial显示文摘Amblyopia resulting from early deprivation of vision or defocus in one eye reflects an imbalance of input from the eyes to the visual cortex.We tested the hypothesis that asynchronous stimulation of the two eyes might induce synaptic plasticity and rebalance input.Experiments on normal adults showed that repetitive brief exposure of grating stimuli,with the onset of each stimulus delayed by 8.3 ms in one eye,results in a shift in perceptual eye dominance.Clinical studies(Clinical trial registration number:Chi CTR2100049130),using popular 3D movies with similar asynchrony between the two eyes(amblyopic eye stimulated first)to treat anisometropic amblyopia,established that just 10.5 h of conditioning over<3 weeks produced improvement that met criteria for successful treatment.The benefits of asynchronous conditioning accumulate over 20–3045 min sessions,and are maintained for at least 2 years.Finally,we demonstrate that asynchronous binocular treatment alone is more effective than patching only.This novel treatment is popular with children and is some 50 times more efficient than patching alone. | Xin Huang Huika Xia Qi Zhang Colin Blakemore Yan Nan Wenyao Wang Jie Gao Spencer S Ng Jing Wen Tiejun Huang Xiaoqing Li Mingliang Pu | 2022 | Science China(Life Sciences)2022，65，3： | 1 |
| 6 | Polymerized placenta hemoglobin attenuates ischemia/reperfusion injury and restores the nitroso-redox balance in isolated rat heart显示文摘 | Tao Li Jing Li Jin Liu Pu Zhang Wei Wu Ronghua Zhou Guohua Li Wensheng Zhang Mingliang Yi Han Huang | 2008 | Free Radical Biology and Medicine2008，，3： | 1 |
| 7 | Functional evaluation of iodoacetic acid induced photoreceptor degeneration in the cat显示文摘Iodoacetic acid(IAA) has been applied to different species to acutely induce photoreceptor degeneration.The purpose of the present study was to use this toxin to thoroughly eliminate photoreceptors and induce complete blindness in the cat.IAA was delivered by single ear vein injection(20 mgkg-1).Six months after the IAA treatment,functional evaluations including pupillary light reflex(PLR),electroretinogram(ERG),visual behavior tests were performed.Morphological examinations were carried out after the functional evaluation.The present result shows that,six months after the IAA application,animals lost visual functions and became completely blind.High dose IAA application via ear vein delivery created an acute and reliable complete photoreceptor degeneration model in the cat.This model can be applied to genetic and cellular therapies for visual function restoration. | NAN Yan ZHANG Qin REN ChaoRan HUANG Xin GAO Jie LI XiaoXin PU MingLiang | 2013 | Science China(Life Sciences)2013，56，6： | 0 |
| 8 | Understanding diabetic retinopathy显示文摘A recent survey shows that diabetes affect 92.4 million people in China's Mainland[1],among which 16.9 million have diabetic retinopathy(DR)[2].DR is one of the major causes of blindness in the working age population in | PU MingLiang | 2015 | Science China(Life Sciences)2015，58，6： | 0 |
| 9 | 缰核相关神经通路可介导光疗的抗抑郁作用显示文摘文章简介视网膜所感知的光信息可调节抑郁情绪在内的多种非成像功能。虽然临床研究表明光疗可显著改善抑郁症状,但光疗抗抑郁作用产生的神经通路机制尚不明确。在本研究中,研究人员通过采用神经环路示踪技术发现在小鼠中存有一条连接视网膜(Retina)与外侧缰核(LHb)的跨双突触神经通路。 | 黄鲁 席月 黄晓丹 Yan Yang Xiaodan Huang Yunwei Fu Qian Tao Jia Xiao Tifei Yuan Kai An Huan Zhao Mingliang Pu Fuqiang Xu Tian Xue Minmin Luo Kwok-Fai So 任超然 苏国辉 | 2020 | 科学新闻2020，，2： | 0 |
| 10 | Design and analysis of the Macao Science Satellite-1's laser retro-reflector array显示文摘The origin and spatial-temporal variation of the Earth’s magnetic field(EMF)is one of the important scientific problems that has long been unsolved.The Macao Science Satellite-1(MSS-1)under construction is China’s first high-precision EMF measurement satellite.To satisfy the highly precise requirements of the MSS-1 orbit measurement,a light,high-precision,four-prism laser retroreflector array was designed.It weighs approximately 285 g,its effective reflection area is greater than 1.77 cm^(2),and its size is 100×100×41 mm.The laser retro-reflector array has excellent performance,and it can achieve a ranging precision at the subcentimeter level for satellite laser ranging.It will be developed and installed on the MSS-1 as a power-free load for high-precision orbit measurement and accurate orbit calibration.The MSS-1 is planned to be brought into the International Laser Ranging Service observations.More than 31satellite laser ranging stations in the International Laser Ranging Service around the world will be able to measure the MSS-1 with long arcs,which will support the scientific mission of high-precision EMF exploration. | ZhiEn Cheng Kai Tang ZhongPing Zhang HaiFeng Zhang Pu Li MingLiang Long | 2023 | Earth and Planetary Physics2023，7，1： | 0 |
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