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| 1 | Characteristics of the microbial communities in the integrated vertical-flow constructed wetlands显示文摘Microorganisms play an important role in removing pollutants from constructed wetlands.We investigated the microbial characteristics in a novel integrated vertical-flow constructed wetland(IVCW) ,which has been in operation in Wuhan,China since 1998.We used phospholipid fatty acid(PLFA) and amoA gene to analyze the structure and diversity of the microbial community within the IVCW.PLFA results suggested that the amount of bacterial PLFA was significantly higher than that of fungal PLFA,but the total microbial biomass represented by PLFA index was low in the system.Microbial spatial distribution showed significantly higher bacterial(both G+and G-) and fungal biomass in the surface than in the subsurface layers.The ratios of monounsaturated to branched PLFA demonstrated that an anaerobic layer sandwiched by two aerobic layers existed in the IVCW,consistent with the redox potential results.Analysis of the amoA revealed the presence of Nitrosomonas-like sequences in the surface substrate of the downflow chamber and apparent diversities of ammonia-oxidizing bacteria in the system.These results suggest that microorganisms,despite their relatively low biomass,have inhabited the IVCW,and the results will offer some valuable information on microbe to system designers and managers. | ZHOU Qiaohong,HE Feng,Zhang Liping,WANG Yanfen,WU Zhenbin State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China Department of Biology and Chemistry,City University of Hong Kong | 2009 | Journal of Environmental Sciences2009，21，9： | 14 |
| 2 | Arabidopsis co-chaperonin CPN20 antagonizes Mg-chelatase H subunit to derepress ABA-responsive WRKY40 transcription repressor显示文摘Our previous study demonstrated that a chloroplast co-chaperonin 20(CPN20),one of the interaction partners of the magnesium-protoporphyrin IX chelatase H subunit(CHLH/ABAR),negatively regulates ABA signaling at the same node with ABAR but upstream of WRKY40 transcription repressor in Arabidopsis thaliana.In the present experiment,we showed that ABA directly inhibits the ABAR-CPN20 interaction,and also represses expression of CPN20,which depends on ABAR.CPN20 inhibits ABAR-WRKY40 interaction by competitively binding to ABAR.ABAR downregulates,but CPN20 upregulates,WRKY40 expression.The cpn20-1 mutation induces downregulation of WRKY40,and suppresses the upregulated level of WRKY40 due to the cch mutation in the ABAR gene.ABA-induced repressive effect of the WRKY40 gene is strengthened by downregulation of CPN20 but reduced by upregulation of CPN20.Together with our previously reported genetic data,we provide evidence that CPN20 functions through antagonizing the ABAR-WRKY40 coupled pathway,and ABA relieves this pathway of repression by inhibiting the ABAR-CPN20 interaction to activate ABAR-WRKY40 interaction. | ZHANG XiaoFeng JIANG Tao YU YongTao WU Zhen JIANG ShangChuan LU Kai FENG XiuJing LIANG Shan LU YanFen WANG XiaoFang ZHANG DaPeng | 2014 | Science China(Life Sciences)2014，57，1： | 11 |
| 3 | CD30 expression in extranodal natural killer/T-cell lymphoma,nasal type among 622 cases of mature T-cell and natural killer-cell lymphoma at a single institution in South China显示文摘Background:Mature T-cell and natural killer(NK)-cell lymphomas compose a heterogeneous group of non-Hodgkin lymphomas,and extranodal NK/T-cell lymphoma,nasal type(ENKTL) is an aggressive subtype with sporadic CD30 expression.However,the significance of CD30 expression in ENKTL is controversial.We aimed to classify a large cohort of patients with mature T-cell and NK-cell lymphomas according to the 2016 World Health Organization(WHO) classification guidelines and to study the association between CD30 expression and prognosis of patients with ENKTL.Methods:We selected consecutive patients with mature T-cell and NK-cell lymphomas who attended our institution between September 1,2009 and August 31,2013.We classified the lymphomas according to the 2016 revision of the WHO classification of lymphoid neoplasms,analyzed the associations between CD30 expression and clinicopathologic features of ENKTL patients,and evaluated the prognostic implications of CD30 expression.Results:We identified 622 consecutive patients with mature T-cell and NK-cell lymphomas,including 317(51.0%)patients with ENKTL.In addition,CD30 expression was detected in 43(47.3%) of a subset of 91 patients with ENKTL.No clinicopathologic features were associated with CD30 expression,and CD30 positivity showed no prognostic significance in patients with ENKTL.Conclusions:ENKTL is the most common type of mature T-cell and NK-cell lymphoma diagnosed at our institution.CD30 is frequently expressed in ENKTL and represents a therapeutic target;however,it may not be a prognostic marker. | Yanfen Feng Huilan Rao Yiyan Lei Yuhua Huang Fang Wang Yu Zhang Shaoyan Xi Qiuliang Wu Jianyong Shao | 2017 | Chinese Journal of Cancer2017，36，6： | 10 |
| 4 | Diversity of culturable heterotrophic bacteria from the Mariana Trench and their ability to degrade macromolecules显示文摘The Mariana Trench is the deepest location on earth and harbors unique microbial communities as evidenced by 16S rRNA gene amplicon and metagenomic sequencing.Obtaining culturable microorganisms from the Mariana Trench will contribute to a further understanding of hadal biogeochemical processes and act as a unique microbial reservoir with potential applications.Here,825 bacterial strains,identified by 16S rRNA gene sequencing,were isolated from 12 water depths(0-10,400 m)of the Mariana Trench with 2216E and R2A media at 4℃ or 28℃ on four cruises during 2015-2017.These bacteria belong to four phyla,nine classes,27 orders,45 families and 108 genera.Alphaproteobacteria,Gammaproteobacteria,Actinobacteria_c,Bacilli and Flavobacteriia were the most abundant classes,accounting for 37.9%,33.0%,11.8%,8.6%and 8.0%of the total bacterial isolates,respectively.2216E and R2A media were found to have a better selectivity to Bacilli and Flavobacteriia,respectively.Fifty strains were potential novel bacterial species with a 16S rRNA gene similarity<98.65%,and a higher percentage of novel strains were obtained from R2A than 2216E medium.Additionally,301(150 species)out of 354 strains(178 species)selected from each depth could degrade at least one of the ten kinds of macromolecules tested.These results indicate that there is a high diversity of culturable bacteria in the Mariana Trench and they can produce a variety of extracellular enzymes.Our study provides a valuable resource of microorganisms for investigating their biogeochemical roles in the Mariana Trench and for industrial applications. | Xiuxiu Zhao Jiwen Liu Shun Zhou Yanfen Zheng Yanhong Wu Kazuhiro Kogure Xiao-Hua Zhang | 2020 | Marine Life Science & Technology2020，2，2： | 5 |
| 5 | Development of high-resolution multiple-SNP arrays for genetic analyses and molecular breeding through genotyping by target sequencing and liquid chip显示文摘Genotyping platforms,as critical supports for genomics,genetics,and molecular breeding,have been well implemented at national institutions/universities in developed countries and multinational seed companies that possess high-throughput,automatic,large-scale,and shared facilities.In this study,we integrated an improved genotyping by target sequencing(GBTS)system with capture-in-solution(liquid chip)technology to develop a multiple single-nucleotide polymorphism(mSNP)approach in which mSNPs can be captured from a single amplicon.From one 40K maize mSNP panel,we developed three types of markers(40K mSNPs,251K SNPs,and 690K haplotypes),and generated multiple panels with various marker densities(1K–40K mSNPs)by sequencing at different depths.Comparative genetic diversity analysis was performed with genic versus intergenic markers and di-allelic SNPs versus non-typical SNPs.Compared with the one-amplicon-one-SNP system,mSNPs and within-mSNP haplotypes are more powerful for genetic diversity detection,linkage disequilibrium decay analysis,and genome-wide association studies.The technologies,protocols,and application scenarios developed for maize in this study will serve as a model for the development of mSNP arrays and highly efficient GBTS systems in animals,plants,and microorganisms. | Zifeng Guo Quannv Yang Feifei Huang Hongjian Zheng Zhiqin Sang Yanfen Xu Cong Zhang Kunsheng Wu Jiajun Tao Boddupalli MPrasanna Michael SOlsen Yunbo Wang Jianan Zhang Yunbi Xu | 2021 | Plant Communications2021，2，6： | 5 |
| 6 | Dual-atom catalysts:controllable synthesis and electrocatalytic applications显示文摘Electrocatalysis,as the nexus for energy storage and environmental remediation,requires developing low-cost and highperforming heterogeneous catalysts.Compared with the single atom catalysts(SACs),dual-atom catalysts(DACs)are attracting ever-increasing interest due to their higher metal loading,more versatile active sites and unique reactivity.However,controlled synthesis of DACs remains a great challenge,and their electrocatalytic applications are still in infancy.This review first discusses the synthesis of DACs by highlighting several synthetic strategies.Subsequently,we exemplify the unique reactivities of DACs in electrocatalytic applications including water splitting,oxygen reduction,carbon dioxide reduction and nitrogen reduction.The structure-activity relations of DACs are specifically discussed in comparison with that of SACs,on the basis of experimental and theoretical studies.Finally,the opportunities and challenges of DACs are summarized in terms of rational design,controlled synthesis,characterization,and applications. | Shengbo Zhang Yanfen Wu Yu-Xiao Zhang Zhiqiang Niu | 2021 | Science China Chemistry2021，64，11： | 4 |
| 7 | A pH-sensitive supramolecular nanosystem with chlorin e6 and triptolide co-delivery for chemo-photodynamic combination therapy显示文摘The combination of Ce6,an acknowledged photosensitizer,and TPL,a natural anticancer agent,has been demonstrated as a useful strategy to reinforce the tumor growth suppression,as well as decrease the systemic side effects compared with their monotherapy.However,in view of the optimal chemo-photodynamic combination efficiency,there is still short of the feasible nanovehicle to steadily co-deliver Ce6 and TPL,and stimuli-responsively burst release drugs in tumor site.Herein,we described the synergistic antitumor performance of a pH-sensitive supramolecular nanosystem,mediated by the host–guest complexing betweenβ-CD and acid pH-responsive amphiphilic co-polymer mPEG-PBAE-mPEG,showing the shell–core structural micelles with the tightβ-CD layer coating.Both Ce6 and TPLwere facilely co-loaded into the spherical supramolecular NPs(TPL+Ce6/NPs)by one-step nanoprecipitation method,with an ideal particle size(156.0 nm),acid pH-responsive drug release profile,and enhanced cellular internalization capacity.In view of the combination benefit of photodynamic therapy and chemotherapy,as well as co-encapsulation in the fabricated pH-sensitive supramolecular NPs,TPL+Ce6/NPs exhibited significant efficacy to suppress cellular proliferation,boost ROS level,lower MMP,and promote cellular apoptosis in vitro.Particularly,fluorescence imaging revealed that TPL+Ce6/NPs preferentially accumulated in the tumor tissue area,with higher intensity than that of free Ce6.As expected,upon 650-nm laser irradiation,TPL+Ce6/NPs exhibited a cascade of amplified synergistic chemo-photodynamic therapeutic benefits to suppress tumor progression in both hepatoma H22 tumor-bearingmice and B16 tumor-bearingmice.More importantly,lower systemic toxicitywas found in the tumor-bearingmice treated with TPL+Ce6/NPs.Overall,the designed supramolecular TPL+Ce6/NPs provided a promising alternative approach for chemo-photodynamic therapy in tumor treatment. | Yihan Wu Jingjing Li Xuemei Zhong Jinfeng Shi Yanfen Cheng Chenglin He Jiaxin Li Liang Zou Chaomei Fu Meiwan Chen Jinming Zhang Huile Gao | 2022 | Asian Journal of Pharmaceutical Sciences2022，17，2： | 4 |
| 8 | Characterization of a Partially Protective B-cell Epitope within the 62 kDa Antigen of Schistosoma japonicum显示文摘约 2 亿个人当前全球受不了血吸虫病,最重要的人的寄生疾病之一。尽管确定的感染能与驱虫剂和 praziquantel 被治疗,种痘将是为不可分的控制的理想的方法血吸虫病。血吸虫 mansoni IrV-5,为研究由世界健康组织 / 专辑计划推荐了为一个疫苗的候选人并且在 TropicalDiseases 训练,在动物模型的生产高保护。我们因此集中于它的相当或相同的事物,血吸虫日本嗯 62 kDa 抗原,并且为 B 房间 epitopes 的预言用 B cell/antibody-related 数据库和分析工具分析了它。Epitope B3 为进一步的调查被选择。用一个鼠科的模型的实验显示与 B3 使免疫的老鼠导致了淋巴细胞增长并且生产了特定的免疫球蛋白 G 和 G1 的高水平,但是没生产出色的 cytokines。种痘显示出部分保护的免疫,对 S 由蠕虫负担和反肥沃免疫测量了。日本嗯。这些结果显示了 epitope B3from S。日本嗯 62-kDa 抗原可能为未来 epitope 疫苗调查充当一张候选人免疫情报。 | Lei ZHANG Xue YANG Yanfen YANG Jiaqing ZHAO Jianghua YANG Feng LIU Zhaosong ZHANG Guanling WU Chuan SU | 2007 | Acta Biochimica et Biophysica Sinica2007，39，10： | 2 |
| 9 | Methane emissions from rice paddies natural wetlands, lakes in China: synthesis new estimate显示文摘 | Huai Chen Qiu’an Zhu Changhui Peng Ning Wu Yanfen Wang Xiuqin Fang Hong Jiang Wenhua Xiang Jie Chang Xiangwen Deng Guirui Yu | 2012 | Glob Change Biol2012，，1： | 1 |
| 10 | Effects of addition of yttrium on properties and microstructure for China low activation martensitic (CLAM) steel 显示文摘 | Li Yanfen Huang Qunying Wu Yican | 2007 | Fusion Engineering and Design2007，82，： | 1 |
| 11 | Morphology evolution of a liquid crystalline polymer in polycarbonate matrix enhanced by the addition of glass beads显示文摘 | Peng Chen Liehuan Wu Yanfen Ding | 2006 | Com- posites science and technology2006，66，： | 1 |
| 12 | Evenness is important in assessing progress towards sustainable development goals显示文摘Sustainable development goals(SDGs)emphasize a holistic achievement instead of cherry-picking a few.However,no assessment has quantitatively considered the evenness among all 17 goals.Here,we propose a systematic method,which first integrates both the evenness and the overall status of all goals,to distinguish the ideal development pathways from the uneven ones and then revisit the development trajectory in China from 2000 to 2015.Our results suggest that,despite the remarkable progress,a bottleneck has occurred in China since 2013 due to the stagnant developments in some SDGs.However,many far-reaching policies in China have been targeting these deficiencies since then,providing a perspective on how a country approaches sustainable development by promoting evenness among all SDGs.Our results also indicate that regions with the slowest progress are the developed provinces,owing to the persistent uneven status of all goals.Our study demonstrates the importance of adopting evenness in assessing and guiding sustainable development. | Yali Liu Jianqing Du Yanfen Wang Xiaoyong Cui Jichang Dong Yanbin Hao Kai Xue Hongbo Duan Anquan Xia Yi Hu Zhi Dong Bingfang Wu Xinquan Zhao Bojie Fu | 2021 | National Science Review2021，8，8： | 1 |
| 13 | Spatial variations on methane emissions from Zoige alpine wetlands of Southwest China显示文摘 | Huai Chen Ning Wu Yongheng Gao Yanfen Wang Peng Luo Jianqing Tian | 2008 | Science of the Total Environment2008，，3： | 1 |
| 14 | Interpretation of Diagnosis and Treatment Protocol for Novel Coronavirus Pneumonia(Trial Version 7)显示文摘On March 3,2020,the National Health Commission and the National Administration of Traditional Chinese Medicine of China jointly promulgated the Notice on Printing and Distributing the Diagnosis and Treatment Protocol for Novel Coronavirus Pneumonia(Trial Version 7).The content has been revised and supplemented on the basis of the Version 6,including etiological characteristics and pathological changes in lung,heart,kidney,spleen,liver,gallbladder,esophagus,stomach and intestinal mucosal epithelium.In particular,the diagnosis and treatment methods of traditional Chinese medicine are supplemented.It provides an evidence-based diagnosis and treatment protocol for the treatment and recovery of suspected and confirmed cases of novel coronavirus pneumonia with traditional Chinese medicine and Western medicine. | Jinfeng CHEN Zhongyi LEI Chaofeng LIU Hong FAN Peng LEI Xueping WU Xiaoyong YU Yanfen ZHOU Jiejun HOU | 2020 | Medicinal Plant2020，11，3： | 1 |
| 15 | The effect of increased atmospheric temperature and CO_2 concentration during crop growth on the chemical composition and in vitro rumen fermentation characteristics of wheat straw显示文摘This experiment was c onducted to investigate the effects of increased atmospheric temperature and CO_2concentration during crop growth on the chemical composition and in vitro rumen fermentation characteristics of wheat straw.The field experiment was carried out from November 2012 to June 2013 at Changshu(31°32'93 'N,120°41'88' E) agro-ecological experimental station.A total of three treatments were set.The concentration of CO_2was increased to 500 umol/mol in the first treatment(CO_2 group).The temperature was increased by 2 ℃ in the second treatment(TEM group) and the concentration of CO_2 and temperature were both increased in the third treatment(CO_2 + TEM group).The mean temperature and concentration of CO_2 in control group were 10.5 ℃ and413 umol/mol.At harvesting,the wheat straws were collected and analyzed for chemical composition and in vitro digestibility.Results showed that dry matter was significantly increased in all three treatments.Ether extracts and neutral detergent fiber were significantly increased in TEM and CO_2 + TEM groups.Crude protein was significantly decreased in CO_2 + TEM group.In vitro digestibility analysis of wheat straw revealed that gas production was significantly decreased in CO_2 and CO_2 + TEM groups.Methane production was significantly decreased in TEM and CO_2 + TEM groups.Ammonia nitrogen and microbial crude protein were significantly decreased in all three treatments.Total volatile fatty acids were significantly decreased in CO_2 and CO_2 + TEM groups.In conclusion,the chemical composition of the wheat straw was affected by temperature and CO_2 and the in vitro digestibility of wheat straw was reduced,especially in the combined treatment of temperature and CO_2. | Xiangyu He Yanping Wu Min Cai Chunlong Mu Weihong Luo Yanfen Cheng Weiyun Zhu | 2015 | Journal of Animal Science and Biotechnology2015，6，4： | 1 |
| 16 | Synthesis of carbon dots with a tunable photoluminescence and their applications for the detection of acetone and hydrogen peroxide显示文摘Carbon dots(CDs) with multi-color emissive properties and a high photoluminescent quantum yield(PLQY) have attracted great attention recently due to their potential applications in chemical,environmental,biological and photo-electronic fields.Solvent-dependent effect in photoluminescence provides a facial and effective approach to tune the emission of CDs.In this study,green emissive nitrogen-doped carbon dots(N-CDs) are synthesized from p-hydroquinone and ethylenediamine through a simple hydrothermal method.The as-prepared N-CDs possess a robust excitation-independent green luminescence and a high PLQY of up to 15.9%.Further spectroscopic characterization indicates that the high PLQY is achieved by the balance of nitrogen doping states and the surface passivation extent in CDs.The N-CDs also exhibit solvent-dependent multi-color emissive property and distinct PLQY in different solvents(the maximum can reach up to 25.3%).Furthermore,the as-prepared N-CDs are applied as fluorescence probes to detect acetone and H2O2 in water.This method has exhibited a low detection limit of acetone(less than 0.1 %) and a quick and linear response to the H_2O_2 with the concentration from 0 to 120 μmol/L.This work broadens the knowledge of applying CDs as probes in the bio and chemical sensing fields. | Shitong Cui Yanfen Wu Yao Liu Qingwen Guan Yida Zhang Yilin Zhang Song Luo Meng Xu Juncheng Wang | 2020 | Chinese Chemical Letters2020，31，2： | 1 |
| 17 | Effects of DMEM and RPMI 1640 on the biological behavior of dog periosteum-derived cells显示文摘 | Xiaohong Wu Minkui Lin Yanfen Li Xin Zhao Fuhua Yan | 2009 | Cytotechnology2009，，2： | 1 |
| 18 | Non-Local DWI Image Super-Resolution with Joint Information Based on GPU Implementation显示文摘Since the spatial resolution of diffusion weighted magnetic resonance imaging(DWI)is subject to scanning time and other constraints,its spatial resolution is relatively limited.In view of this,a new non-local DWI image super-resolution with joint information method was proposed to improve the spatial resolution.Based on the non-local strategy,we use the joint information of adjacent scan directions to implement a new weighting scheme.The quantitative and qualitative comparison of the datasets of synthesized DWI and real DWI show that this method can significantly improve the resolution of DWI.However,the algorithm ran slowly because of the joint information.In order to apply the algorithm to the actual scene,we compare the proposed algorithm on CPU and GPU respectively.It is found that the processing time on GPU is much less than on CPU,and that the highest speedup ratio to the traditional algorithm is more than 26 times.It raises the possibility of applying reconstruction algorithms in actual workplaces. | Yanfen Guo Zhe Cui Zhipeng Yang Xi Wu Shaahin Madani | 2019 | Computers, Materials & Continua2019，，9： | 0 |
| 19 | Discussion on the Selection of ACEI and ARB for the Patients with COVID-19 and Hypertension from ACE2显示文摘With the wide spread of COVID-19,some studies have confirmed that novel coronavirus enters the cell through the binding of spike protein and ACE2 protein,which has the risk of causing the virus to enter the cell to accelerate its transmission.ACEI and ARB are the key drugs for the treatment of hypertension and are widely used in clinic.They are good for ventricular and vascular remodeling in patients with hypertension,coronary heart disease and heart failure.ACEI and ARB drugs may increase the expression of ACE2 in lung tissue and increase the risk of aggravation of the disease.According to the advice of hypertension experts:for mild ordinary COVID-19 patients with hypertension,we can consider discontinuing ACEI and ARB drugs,while for severe COVID-19 patients with hypertension,ACEI and ARB drugs should be stopped immediately.During the discontinuation period,temporary replacement therapy with diuretics,dipine and vasodilatation hypotensive drugs can be considered. | Jinfeng CHEN Zhongyi LEI Chaofeng LIU Hong FAN Peng LEI Xueping WU Xiaoyong YU Yanfen ZHOU Jiejun HOU | 2020 | Medicinal Plant2020，11，4： | 0 |
| 20 | Discussion on the Prevention and Treatment of COVID-19 Causing Lung Disease and Heart Damage Based on Lei Zhongyi's Theory of Intermingled Phlegm,Blood Stasis and Toxin显示文摘Novel coronavirus infection not only damages lung function,but also causes myocardial injury,elevated myocardial enzymes and heart failure,especially for patients with basic heart diseases who develop COVID-19,the first consideration should be the protection of cardiac function.Based on the theory of intermingled phlegm,blood stasis and toxin of heart disease put forward by Master Lei Zhongyi,the dialectical treatment thinking of COVID-19 patients from the concept of damage of phlegm,blood stasis and toxin to the heart were discussed.During the diagnosis,critical stage and recovery period of COVID-19,expectorant and blood-activating agents,heat and detoxification agents can be added to promote lung and asthma,free Bizheng and remove blood stasis,calm the heart and calm the mind,and promote the recovery of cardiopulmonary functions. | Jinfeng CHEN Zhongyi LEI Chaofeng LIU Hong FAN Peng LEI Xueping WU Xiaoyong YU Yanfen ZHOU Jiejun HOU | 2020 | Medicinal Plant2020，11，6： | 0 |
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