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| 1 | Arabidopsis co-chaperonin CPN20 antagonizes Mg-chelatase H subunit to derepress ABA-responsive WRKY40 transcription repressor显示文摘Our previous study demonstrated that a chloroplast co-chaperonin 20(CPN20),one of the interaction partners of the magnesium-protoporphyrin IX chelatase H subunit(CHLH/ABAR),negatively regulates ABA signaling at the same node with ABAR but upstream of WRKY40 transcription repressor in Arabidopsis thaliana.In the present experiment,we showed that ABA directly inhibits the ABAR-CPN20 interaction,and also represses expression of CPN20,which depends on ABAR.CPN20 inhibits ABAR-WRKY40 interaction by competitively binding to ABAR.ABAR downregulates,but CPN20 upregulates,WRKY40 expression.The cpn20-1 mutation induces downregulation of WRKY40,and suppresses the upregulated level of WRKY40 due to the cch mutation in the ABAR gene.ABA-induced repressive effect of the WRKY40 gene is strengthened by downregulation of CPN20 but reduced by upregulation of CPN20.Together with our previously reported genetic data,we provide evidence that CPN20 functions through antagonizing the ABAR-WRKY40 coupled pathway,and ABA relieves this pathway of repression by inhibiting the ABAR-CPN20 interaction to activate ABAR-WRKY40 interaction. | ZHANG XiaoFeng JIANG Tao YU YongTao WU Zhen JIANG ShangChuan LU Kai FENG XiuJing LIANG Shan LU YanFen WANG XiaoFang ZHANG DaPeng | 2014 | Science China(Life Sciences)2014，57，1： | 11 |
| 2 | Targeting glutamine metabolism in PIK3CA mutant colorectal cancers显示文摘We recently reported that PIK3CA mutant colorectal cancers(CRCs)are addicted to glutamine through up-regulation of glutamate pyruvate transaminase 2(GPT2).A GPT2 inhibitor suppresses in vivo growth of PIK3CA mutant,but not wild-type,CRCs.This study indicates that targeting glutamine may be an effective approach to treat CRCs with PIK3CA mutations. | Xiujing Feng Yujun Hao Zhenghe Wang | 2016 | Genes & Diseases2016，3，4： | 4 |
| 3 | Proteome identification of binding-partners interacting with cell polarity protein Par3 in Jurkat cells显示文摘evolutionarily 保存的房间极性蛋白质 Par3,像支架的 PDZreontaining 蛋白质,在上皮的房间极性的建立和维护起一个关键作用。尽管在在上皮的房间建立房间极性的 Par3 的角色强烈地被探索了,在造血的房间的 Par3 的功能留下逃犯。处理这个问题,我们产生了 Par3 PDZ 领域的 GST 熔化蛋白质。由把 GST-pull-down 途径与液体层析双人脚踏车团 spectrometry 相结合,我们在 Jurkat 房间(一根 T 房间线) 识别了 Par3 的 PDZ 领域的 10 潜在的新奇有约束力的蛋白质。有三蛋白质的 Par3 的相互作用—原子运输蛋白质 importin-α4 和 proteasome 使活跃之物 PA28β 和 PA28γ—被证实在 vitro 绑定试金, co-immunoprecipitation 试金和免疫荧光显微镜学使用。我们的结果有潜力揭开在血房间的房间极性蛋白质 Par3 的新奇功能。 | Ying Zhou Longhou Fang Dan Du Wenchao Zhou Xiujing Feng Jiwu Chen Zhe Zhang Zhengjun Chen | 2008 | Acta Biochimica et Biophysica Sinica2008，40，8： | 2 |
| 4 | FGF-receptor substrate 2 functions as a molecular sensor ntegrating external regulatory signals into the FGF pathway显示文摘(FRS2 ) 成纤维细胞生长因素(FGF ) 受体底层 2 是在 FGF 小径发信号的主要调停人。最近的研究在 FRS2 显示出那激活 mitogen 的蛋白质 kinase (MAPK ) phosphorylates 丝氨酸和 threonine 残余,否定地影响 FRS2 的导致 FGF 的酷氨酸 phosphorylation (PY ) 。几种刺激能导致 FRS2 的 serine/threonine phosphorylation (PS/T ) ,显示 FRS2 可能为学习在表明小径的生长因素之间的串音是有用的。这里,我们报导 FRS2 的导致 FGF 的 PY 能被 EGF 合作刺激在 PC12 房间稀释;这禁止的效果能被 U0126 完全颠倒, MEK 的一个禁止者。我们进一步在 FRS2 识别了 ERK1/2-binding 主题并且在 FGF 或 EGF 刺激之上产生了 FRS2-3KL,变异的缺乏 MAPK 绑定和磅。不同野类型(WT ) FRS2, FRS2-3KL 的导致 FGF 的 PY 不能被 EGF 合作刺激,和更展出的 FRS2-3KL-expressing PC12 房间禁止响应 FGF 处理比 FRS2-WT-expressing 房间区分潜力。这些结果建议 FRS2 的 PS/T 由 FRS2-MAPK 否定规章的循环调停了可以作为从另外的小径把否定规章的信号集成到产生 FGFR 的信号 transduction 的一个分子的开关工作。 | Wenchao Zhou Xiujing Feng Yingjie Wu Johannes Benge Zhe Zhang Zhengjun Chen | 2009 | Cell Research2009，19，10： | 2 |
| 5 | Effect of mushroom root fermentation broth on the umami taste and nutrients of Flammulina velutipes显示文摘As one of the most appealing edible mushrooms,the quality of Flammulina velutipes can be affected by the cultivation substrate,which has an impact on the mushroom umami taste and nutrients.In our study,the effect of mushroom root fermentation broth(MRFB)based substrate on umami taste and nutrients of F.velutipes were evaluated;four proportions of MRFB were conducted(CK 0%,E16.7%,E213.4%,E320.1%).Results indicated that MRFB was an effective nutrient supplement for mushroom cultivation substrate.E2 and E3 showed higher crude fiber,crude fat,and soluble protein.Compared to CK,the content of monosodium glutamate-like(MSG-like)amino acids,essential amino acids,total amino acids,and total 5′-nucleotides in E2 and E3 were higher.The equivalent umami concentration(EUC)values in E1,E2 and E3 were 1.70,2.43 and 1.56 times that of CK,respectively.Higher umami,saltness and sweetness taste values were found in E2.Thereby,it signified that better umami and richer nutrients were achieved by using substrate with proper volume of MRFB,especially,E2 with 13.4%of MRFB.Overall,better mushroom quality could be derived from the application of MRFB in cultivation.MRFB was a favorable nutrient supplement for F.velutipes cultivation substrate. | Zijian Wang Xiujing Bao Rongrong Xia Zhenshan Hou Yunting Li Yao Feng Yafei Wang Song Pan Heran Xu Zhuqing Huang Guang Xin | 2023 | Journal of Future Foods2023，3，1： | 0 |
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