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| 1 | BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity显示文摘基础编辑() ,包含 cytidine 脱氨基酶和催化地有缺点的 Cas9,广泛地被用来执行基础编辑。然而,狭窄的编辑窗口是限制它的实用程序。这里,我们开发了依照到由熔化 10 的 U (T) 范围(加) 的更大的 C 为招募 GCN4 肽拷贝到 nCas9 (D10A ) 的编程作为基础编辑命名的一种新编辑技术 scFv-APOBEC-UGI-GB1 到目标地点。新系统完成与一扇拓宽的窗户的基础编辑,导致一个增加的指向染色体的范围。有趣地,新系统很产出更少讨厌的 indels 和 non-C-to-T 变换。我们也通过站 codons (iSTOP ) 的正式就职越过整个染色体在基因混乱表明了它的潜在的使用。一起拿,加系统与编辑窗口和提高的忠实增加提供一个新编辑工具。 | Wen Jiang Songjie Feng Shisheng Huang Wenxia Yu Guanglei Li Guang Yang Yajing Liu Yu Zhang Lei Zhang Yu Hou Jia Chen Jieping Chen Xingxu Huang | 2018 | Cell Research2018，28，8： | 20 |
| 2 | Induced earthquakes in the development of unconventional energy resources显示文摘It has long been known that human activities such as waste fluid disposal and reservoir impoundment may cause earthquakes. Recently, anthropogenic activities to tackle the increasing energy demand and to address climate change issues are also reported to induce earthquakes. These activities have a common attribute in that fluids are injected and extracted underground and induce spatiotemporal changes of pore pressure and stress, which may cause slip on faults. Induced earthquakes not only pose significant impacts on seismic hazard assessment and preparation, but also raise the question to the society as how to balance the economic needs of resources development and the public's concerns about potential environmental impacts. Here we review the observations of fluid-injection/extraction induced earthquakes, ground deformation associated with these activities, and their physical mechanisms. Furthermore, we discuss the influences of induced earthquakes on seismic hazard models, regulatory policies on these anthropogenic activities, and current development of academic, industrial and government initiatives and collaborations in order to understand this intriguing phenomenon and address associated challenges. | YANG HongFeng LIU YaJing WEI Meng ZHUANG JianCang ZHOU ShiYong | 2017 | Science China Earth Sciences2017，60，9： | 12 |
| 3 | Neurovascular unit in diabetic retinopathy:pathophysiological roles and potential therapeutical targets显示文摘Diabetic retinopathy(DR),one of the common complications of diabetes,is the leading cause of visual loss in working-age individuals in many industrialized countries.It has been traditionally regarded as a purely microvascular disease in the retina.However,an increasing number of studies have shown that DR is a complex neurovascular disorder that affects not only vascular structure but also neural tissue of the retina.Deterioration of neural retina could precede microvascular abnormalities in the DR,leading to microvascular changes.Furthermore,disruption of interactions among neurons,vascular cells,glia and local immune cells,which collectively form the neurovascular unit,is considered to be associated with the progression of DR early on in the disease.Therefore,it makes sense to develop new therapeutic strategies to prevent or reverse retinal neurodegeneration,neuroinflammation and impaired cell-cell interactions of the neurovascular unit in early stage DR.Here,we present current perspectives on the pathophysiology of DR as a neurovascular disease,especially at the early stage.Potential novel treatments for preventing or reversing neurovascular injuries in DR are discussed as well. | Shen Nian Amy C.Y.Lo Yajing Mi Kai Ren Di Yang | 2021 | Eye and Vision2021，8，1： | 9 |
| 4 | The Optimization of Ultrasonic Extraction Process of Total Flavonoids from Rhodiola Crenulata by Response Surface Method显示文摘[Objectives]To study the optimal extraction process of total flavonoids in Rhodiola rosea L.,and further facilitate the development and utilization of Rhodiola rosea L. [Methods]With Rhodiola crenulata as raw material,ethanol as extractant,ultrasonic extraction as the extraction method,the single factor method was first used for preliminary investigation of effect of ethanol volume fraction,solid-liquid ratio,extraction temperature and extraction time on the flavonoids extraction rate,and then the Box-Behnken response surface method was used to optimize the extraction process of total flavonoids in R. crenulata. [Results] The optimal extraction conditions: ethanol concentration of 72%;solid-liquid ratio of 1∶ 43; extraction temperature of 66℃; extraction time of 50 min. Under these conditions,the extraction rate of total flavonoids from R. crenulata was 2. 591%. [Conclusions] The results showed that the method of response surface was reasonable and feasible for the optimization of ultrasonic extraction of total flavonoids from R. crenulata. | Xuedong GU Yajing SUN Changzhong MA Fumin CHI Lin YANG | 2017 | Medicinal Plant2017，8，6： | 9 |
| 5 | Highly efficient and precise base editing in discarded human tripronuclear embryos显示文摘 | Guanglei Li Yajing Liu Yanting Zeng Jianan Li Lijie Wang Guang Yang Dunjin Chen Xiaoyun Shang Jia Chen Xingxu Huang Jianqiao Liu | 2017 | Protein & Cell2017，8，10： | 8 |
| 6 | Protective effect of Xuebijing injection on myocardial injury in patients with sepsis: a randomized clinical trial显示文摘OBJECTIVE: To investigate the protective effect and possible mechanism of Xuebijing Injection on myocardial injury in patients with sepsis, and to evaluate its prognostic implications.METHODS: Patients with septic myocardial injury were recruited, and were randomly divided into two groups: treatment group and control group. All patients in two groups received conventional cluster treatment, the patients in treatment group additional received Xuebijing injection dissolved in0.9% sodium chloride injection, and the patients in control group received the same amount of 0.9%sodium chloride injection. At the beginning of treatment and 3, 7 and 10-day after treatment, lab-oratory indicators of cardiac troponin Ⅰ(cTnI),N-terminal pro B-type natriuretic peptide(NT-pro BNP) and procalcitonin(PCT) were respectively tested in venous blood. The patient's length of stay in Intensive Care Unit(ICU) and the mortality in 28 days were recorded.RESULTS: At 3, 7 and 10-day after treatment, the improvements of c Tn I, NT-pro BNP and PCT in treatment group were better than those in control group, and the differences were statistically significant(P < 0.05). The mortality of treatment group in28 days was not significantly different from that of control group(P > 0.05). The ICU length of stay of treatment group was shorter than that of control group(P > 0.05).CONCLUSION: Xuebijing injection could improve the levels of c Tn I, NT-pro BNP and PCT in patients with septic myocardial injury.and it had a protective effect on myocardial injury. | Zhang Hongwei Wei Liyou Zhao Gang Liu Shuzheng Zhang Zhenyu Zhang Jing Yang Yajing | 2016 | Journal of Traditional Chinese Medicine2016，36，6： | 7 |
| 7 | Effects of feeding untreated, pasteurized and acidified waste milk and bunk tank milk on the performance, serum metabolic profiles, immunity, and intestinal development in Holstein calves显示文摘Background: The present experiment was performed to assess the effects of different sources of milk on the growth performance, serum metabolism, immunity, and intestinal development of calves. Eighty-four Holstein male neonatal calves were assigned to one of the following four treatment groups: those that received bunk tank milk(BTM), untreated waste milk(UWM), pasteurized waste milk(PWM), and acidified waste milk(AWM) for 21 d.Results: Calves in the BTM and AWM groups consumed more starter(P < 0.05) than those in the UWM group.Average daily gain in the UWM group was the highest(P < 0.05). Calves exhibited the highest(P < 0.05) serum total protein, albumin, total cholesterol, high density lipoprotein, triglycerides, growth hormone, immunoglobulin(Ig) A and Ig M concentrations in the UWM group, highest malondialdehyde and tumor necrosis factor-α in the PWM group(P < 0.05), and highest glutathione peroxidase and Ig G in the BTM group(P < 0.05). The jejunum and ileum of the calves in all treatments presented a slight inflammatory response. The jejunal inflammation scores were higher(P < 0.05) in the UWM and AWM groups than the BTM group; the ileal inflammation scores increased more(P < 0.05) in the AWM group than the BTM group. Jejunal immunohistochemical scores(IHS) were higher(P < 0.05) in the PWM and AWM groups than the BTM group. Compared to the other three groups, calves feeding on BTM had lower(P < 0.05) ileal IHS. Jejunal interleukin(IL)-1β, IL-8, and IL-10 mRNA expression in the UWM group was the highest(P < 0.05). Calves fed AWM increased(P < 0.05) mRNA expression of IL-8 and toll like receptor 4(TLR-4) in the jejunum and IL-8, IL-1β, IL-6, IL-8, and IL-10 in the mesenteric lymph nodes.Conclusions: Overall, bunk tank milk is the best choice for calf raising compared to waste milk. The efficiency of feeding pasteurized and acidified waste milk are comparable, and the acidification of waste milk is an acceptable labor-saving and diarrhea-preventing feed for young calves. | Yang Zou Yajing Wang Youfei Deng Zhijun Cao Shengli Li Jiufeng Wang | 2017 | Journal of Animal Science and Biotechnology2017，8，4： | 7 |
| 8 | An analytical biomarker for treatment of patients with recurrent B-ALL after remission induced by infusion of anti-CD19 chimeric antigen receptor T(CAR-T) cells显示文摘Anti-CD19 chimeric antigen receptor-modified T(CAR-T-19) cells have emerged as a powerful targeted immunotherapy for B-cell lineage acute lymphoblastic leukemia with a remarkable clinical response in recent trials. Nonetheless, few data are available on the subsequent clinical monitoring and treatment of the patients, especially those with disease recurrence after CAR-T-19 cell infusion. Here, we analyzed three patients who survived after our phase I clinical trial and who were studied by means of biomarkers reflecting persistence of CAR-T-19 cells in vivo and predictive factors directing further treatment. One patient achieved 9-week sustained complete remission and subsequently received an allogeneic hematopoietic stem cell transplant. Another patient who showed relapse after 20 weeks without detectable leukemia in the cerebrospinal fluid after CAR-T-19 cell treatment was able to achieve a morphological remission under the influence of stand-alone low-dose chemotherapeutic agents. The third patient gradually developed extensive extramedullary involvement in tissues with scarce immune-cell infiltration during a long period of hematopoietic remission after CAR-T-19 cell therapy. Long-term and discontinuous increases in serum cytokines(mainly interleukin 6 and C-reactive protein) were identified in two patients(Nos. 1 and 6) even though only a low copy number of CAR molecules could be detected in their peripheral blood. This finding was suggestive of persistent functional activity of CAR-T-19 cells. Combined analyses of laboratory biomarkers with their clinical manifestations before and after salvage treatment showed that the persistent immunosurveillance mediated by CAR-T-19 cells would inevitably potentiate the leukemia-killing effectiveness of subsequent chemotherapy in patients who showed relapse after CAR-T-19-induced remission. | Yajing Zhang Wenying Zhang Hanren Dai Yao Wang Fengxia Shi Chunmeng Wang Yelei Guo Yang Liu Meixia Chen Kaichao Feng Yan Zhang Chuanjie Liu Qingming Yang Suxia Li Weidong Han | 2016 | Science China(Life Sciences)2016，59，4： | 6 |
| 9 | Recombinant rabies virus expressing IL-15 enhances immunogenicity through promoting the activation of dendritic cells in mice显示文摘Rabies remains a public health threat that kills approximately 59,000 people worldwide each year,most of which are from the developing countries of Africa and Asia where dog rabies are endemic.Therefore, developing an affordable and efficacious vaccine is crucial for rabies control in these countries. Interleukin(IL)-15, an immunoregulatory cytokine, is a pluripotent molecule with therapeutic potential, which targets many cell types and links the innate and adaptive immune system. In this study, IL-15 gene was cloned and inserted into the genome of a recombinant rabies virus(RABV) strain LBNSE(designated as LBNSE-IL15), and the effect of over-expression of IL-15 on the immunogenicity of RABV was investigated. It was found that mice vaccinated with LBNSEIL15 could induce significantly higher level of virus-neutralizing antibody(VNA) than those immunized with LBNSE, resulting in the higher protection after challenge. Further investigation was performed to find out the possible role of IL-15 plays in the process of antibody induction, and it was found that LBNSE-IL15 could enhance the maturation of dendritic cells(DCs) in immunized mice. Furthermore, the mice immunized with LBNSE-IL15 could promote the T_(FH) cells differentiation and the generation of germinal center B cells and plasma cells. Together, these data indicated that IL-15 could be a potential adjuvant in enhancing the immunogenicity of RABV, contributing to the development of more-efficacious rabies vaccines. | Tiange Chen Yajing Zhang Zhao Wang Jie Yang Mingming Li Kunlun Wang Min Cui Zhen F.Fu Ling Zhao Ming Zhou | 2017 | Virologica Sinica2017，32，4： | 5 |
| 10 | Multifunctional Flexible Humidity Sensor Systems Towards Noncontact Wearable Electronics显示文摘In the past decade,the global industry and research attentions on intelligent skin-like electronics have boosted their applications in diverse fields including human healthcare,Internet of Things,human–machine interfaces,artificial intelligence and soft robotics.Among them,flexible humidity sensors play a vital role in noncontact measurements relying on the unique property of rapid response to humidity change.This work presents an overview of recent advances in flexible humidity sensors using various active functional materials for contactless monitoring.Four categories of humidity sensors are highlighted based on resistive,capacitive,impedance-type and voltage-type working mechanisms.Furthermore,typical strategies including chemical doping,structural design and Joule heating are introduced to enhance the performance of humidity sensors.Drawing on the noncontact perception capability,human/plant healthcare management,human-machine interactions as well as integrated humidity sensor-based feedback systems are presented.The burgeoning innovations in this research field will benefit human society,especially during the COVID-19 epidemic,where cross-infection should be averted and contactless sensation is highly desired. | Yuyao Lu Geng Yang Yajing Shen Huayong Yang Kaichen Xu | 2022 | Nano-Micro Letters2022，14，9： | 5 |
| 11 | Arterial stiffness and stroke:de-stiffening strategy,a therapeutic target for stroke显示文摘Stroke is the second leading cause of mortality and morbidity worldwide.Early intervention is of great importance in reducing disease burden.Since the conventional risk factors cannot fully account for the pathogenesis of stroke,it is extremely important to detect useful biomarkers of the vascular disorder for appropriate intervention.Arterial stiffness,a newly recognised reliable feature of arterial structure and function,is demonstrated to be associated with stroke onset and serve as an independent predictor of stroke incidence and poststroke functional outcomes.In this review article,different measurements of arterial stiffness,especially pressure wave velocity,were discussed.We explained the association between arterial stiffness and stroke occurrence by discussing the secondary haemodynamic changes.We reviewed clinical data that support the prediction role of arterial stiffness on stroke.Despite the lack of long-term randomised double-blind controlled therapeutic trials,it is high potential to reduce stroke prevalence through a significant reduction of arterial stiffness(which is called de-stiffening therapy).Pharmacological interventions or lifestyle modification that can influence blood pressure,arterial function or structure in either the short or long term are promising de-stiffening therapies.Here,we summarised different de-stiffening strategies including antihypertension drugs,antihyperlipidaemic agents,chemicals that target arterial remodelling and exercise training.Large and well-designed clinical trials on de-stiffening strategy are needed to testify the prevention effect for stroke.Novel techniques such as modern microscopic imaging and reliable animal models would facilitate the mechanistic analyses in pathophysiology,pharmacology and therapeutics. | Yajing Chen Fanxia Shen Jianrong Liu Guo-Yuan Yang | 2017 | Stroke & Vascular Neurology2017，2，2： | 3 |
| 12 | 2022 Chinese expert consensus and guidelines on clinical management of toxicity in anti-CD19 chimeric antigen receptor T-cell therapy for B-cell non-Hodgkin lymphoma显示文摘Adoptive cellular immunotherapy with chimeric antigen receptor(CAR)T cells has emerged as a novel modality for treating relapsed and/or refractory B-cell non-Hodgkin lymphoma(B-NHL).With increasing approval of CAR T-cell products and advances in CAR T cell therapy,CAR T cells are expected to be used in a growing number of cases.However,CAR T-cell-associated toxicities can be severe or even fatal,thus compromising the survival benefit from this therapy.Standardizing and studying the clinical management of these toxicities are imperative.In contrast to other hematological malignancies,such as acute lymphoblastic leukemia and multiple myeloma,anti-CD19 CAR T-cell-associated toxicities in B-NHL have several distinctive features,most notably local cytokine-release syndrome(CRS).However,previously published guidelines have provided few specific recommendations for the grading and management of toxicities associated with CAR T-cell treatment for B-NHL.Consequently,we developed this consensus for the prevention,recognition,and management of these toxicities,on the basis of published literature regarding the management of anti-CD19 CAR T-cell-associated toxicities and the clinical experience of multiple Chinese institutions.This consensus refines a grading system and classification of CRS in B-NHL and corresponding measures for CRS management,and delineates comprehensive principles and exploratory recommendations for managing anti-CD19 CAR T-cell-associated toxicities in addition to CRS. | Ping Li Yang Liu Yun Liang Jian Bo Sujun Gao Yongxian Hu Yu Hu He Huang Xiaojun Huang Hongmei Jing Xiaoyan Ke Jianyong Li Yuhua Li Qifa Liu Peihua Lu Heng Mei Ting Niu Yongping Song Yuqin Song Liping Su Sanfang Tu Jianxiang Wang Depei Wu Zhao Wang Kailin Xu Zhitao Ying Qingming Yang Yajing Zhang Fengxia Shi Bin Zhang Huilai Zhang Xi Zhang Mingfeng Zhao Weili Zhao Xiangyu Zhao Liang Huang Jun Zhu Wenbin Qian Weidong Han Aibin Liang | 2023 | Cancer Biology & Medicine2023，20，2： | 3 |
| 13 | Micro-rocket robot with all-optic actuating and tracking in blood显示文摘Micro/nanorobots have long been expected to reach all parts of the human body through blood vessels for medical treatment or surgery.However,in the current stage,it is still challenging to drive a microrobot in viscous media at high speed and difficult to observe the shape and position of a single microrobot once it enters the bloodstream.Here,we propose a new micro-rocket robot and an all-optic driving and imaging system that can actuate and track it in blood with microscale resolution.To achieve a high driving force,we engineer the microrobot to have a rocket-like tripletube structure.Owing to the interface design,the 3D-printed micro-rocket can reach a moving speed of 2.8 mm/s(62 body lengths per second)under near-infrared light actuation in a blood-mimicking viscous glycerol solution.We also show that the micro-rocket robot is successfully tracked at a 3.2-μm resolution with an optical-resolution photoacoustic microscope in blood.This work paves the way for microrobot design,actuation,and tracking in the blood environment,which may broaden the scope of microrobotic applications in the biomedical field. | Dengfeng Li Chao Liu Yuanyuan Yang Lidai Wang Yajing Shen | 2020 | Light(Science & Applications)2020，9，1： | 3 |
| 14 | Synthesis of Single-Crystalline Anatase TiO_2 Nanorods With High-Performance Dye-Sensitized Solar Cells显示文摘Single-crystalline anatase TiO_2 nanorods have been prepared by solvothermal method using tetrabutylammonium hydroxide(TBAH) as a morphology controlling agent.The obtained TiO_2 nanorods are dominated by a large percentage of {010} facets.The power conversion efficiency of dye-sensitized solar cell(DSSC) based on anatase TiO_2 nanorods(8.66%) exhibits a significant improvement(35%) compared to that of P25 TiO_2(5.66%).The high performance of the anatase TiO_2 nanorods solar cell is ascribed to their large percent of exposed {010} facets as well as balancing their surface areas and sizes. | Jin Liu Jun Luo Weiguang Yang Yali Wang Linyan Zhu Yueyang Xu Ying Tang Yajing Hu Chen Wang Yigang Chen Weimin Shi | 2015 | Journal of Materials Science & Technology2015，31，1： | 2 |
| 15 | Tet2 Regulates Osteoclast Differentiation by Interacting with Runx1 and Maintaining Genomic 5-Hydroxymethylcytosine(5hmC)显示文摘As a dioxygenase, Ten-Eleven Translocation 2(TET2) catalyzes subsequent steps of 5-methylcytosine(5 mC) oxidation. TET2 plays a critical role in the self-renewal, proliferation,and differentiation of hematopoietic stem cells, but its impact on mature hematopoietic cells is not well-characterized. Here we show that Tet2 plays an essential role in osteoclastogenesis. Deletion of Tet2 impairs the differentiation of osteoclast precursor cells(macrophages) and their maturation into bone-resorbing osteoclasts in vitro. Furthermore, Tet2^(-/-) mice exhibit mild osteopetrosis, accompanied by decreased number of osteoclasts in vivo. Tet2 loss in macrophages results in the altered expression of a set of genes implicated in osteoclast differentiation, such as Cebpa, Mafb, and Nfkbiz. Tet2 deletion also leads to a genome-wide alteration in the level of 5-hydroxymethylcytosine(5 hmC) and altered expression of a specific subset of macrophage genes associated with osteoclast differentiation. Furthermore, Tet2 interacts with Runx1 and negatively modulates its transcriptional activity. Our studies demonstrate a novel molecular mechanism controlling osteoclast differentiation and function by Tet2, that is, through interactions with Runx1 and the maintenance of genomic 5 hmC. Targeting Tet2 and its pathway could be a potential therapeutic strategy for the prevention and treatment of abnormal bone mass caused by the deregulation of osteoclast activities. | Yajing Chu Zhigang Zhao David Wayne Sant Ganqian Zhu Sarah M. Greenblatt Lin Liu Jinhuan Wang Zeng Cao Jeanette Cheng Tho Shi Chen Xiaochen Liu Peng Zhang Jaroslaw P. Maciejewski Stephen Nimer Gaofeng Wang Weiping Yuan Feng-Chun Yang Mingjiang Xu | 2018 | Genomics, Proteomics & Bioinformatics2018，16，3： | 2 |
| 16 | The model of cytokine release syndrome in CAR T-cell treatment for B-cell non-Hodgkin lymphoma显示文摘Chimeric antigen receptor T(CAR T)cell therapy has demonstrated efficacy in the treatment of haematologic malignancies.However,the accompanying adverse events,the most common of which is cytokine release syndrome(CRS),substantially limit its wide application.Due to its unique physiological characteristics,CRS in CAR T-cell treatment for B-cell non-Hodgkin lymphoma(BNHL)may exhibit some special features.Although existing guidelines had greatly promoted the recognition and management of CRS,many recommendations are not fully applicable to B-NHL.Therefore,it is imperative to identify responses that are specific to CRS observed following CAR T treatment for B-NHL.Based on underlying biological processes and known pathophysiological mechanisms,we tentatively propose a new model to illustrate the occurrence and evolution of CAR T-cell-therapy-related CRS in BNHL.In this model,tumour burden and bone marrow suppression are considered determinants of CRS.Novel phenomena after CAR T-cell infusion(such as local inflammatory response)are further identified.The proposed model will help us better understand the basic biology of CRS and recognize and manage it more rationally. | Jianshu Wei Yang Liu Chunmeng Wang Yajing Zhang Chuan Tong Guanghai Dai Wei Wang John E.J.Rasko J.Joseph Melenhorst Wenbin Qian Aibin Liang Weidong Han | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 2 |
| 17 | Ascorbate peroxidase 1 confers resistance to southern corn leaf blight in maize显示文摘Southern corn leaf blight (SCLB), caused by Bipolarismaydis, is one of the most devastatingdiseases affecting maize production. However,only one SLCB resistance gene, conferring partialresistance, is currently known, underscoring theimportance of isolating new SCLB resistancerelatedgenes. Here, we performed a comparativeproteomic analysis and identified 258 proteinsshowing differential abundance during the maizeresponse to B. maydis. These proteins included anascorbate peroxidase (Zea mays ascorbate peroxidase1 (ZmAPX1)) encoded by a gene locatedwithin the mapping interval of a previously identifiedquantitative trait locus associated with SCLBresistance. ZmAPX1 overexpression resulted inlower H_(2)O_(2) accumulation and enhanced resistanceagainst B. maydis. Jasmonic acid (JA)contents and transcript levels for JA biosynthesisand responsive genes increased in ZmAPX1-overexpressing plants infected with B. maydis,whereas Zmapx1 mutants showed the oppositeeffects. We further determined that low levels of H_(2)O_(2) are accompanied by an accumulation of JAthat enhances SCLB resistance. These resultsdemonstrate that ZmAPX1 positively regulatesSCLB resistance by decreasing H_(2)O_(2) accumulationand activating the JA-mediated defensesignaling pathway. This study identified ZmAPX1as a potentially useful gene for increasing SCLBresistance. Furthermore, the generated datamay be relevant for clarifying the functions ofplant APXs. | Jinghua Zhang Xingmeng Jia Guan-Feng Wang Shijun Ma Shunxi Wang Qin Yang Xueyan Chen Yuqian Zhang Yajing Lyu Xiaoxu Wang Jiawei Shi Yangtao Zhao Yanhui Chen Liuji Wu | 2022 | Journal of Integrative Plant Biology2022，64，6： | 2 |
| 18 | 2021 Chinese consensus on the diagnosis and management of primary immune thrombocytopenia in pregnancy显示文摘Immune thrombocytopenia(ITP)is an acquired disease characterized by isolated thrombocytopenia,which is one of the most common causes of thrombocytopenia during pregnancy.Women with ITP who have severe thrombocytopenia are at an increased risk for life-threatening obstetric complications.Therefore,we established this consensus statement on the diagnosis and management of ITP during pregnancy(detailed information is available in the Supplementary File,http://gffzz16e312a87a7141a5hkf0xb6pucn6v6vvq.ffgz.tsg.suse.edu.cn/A978). | Zhang Xiaohui Chen Fangping Chen Xiequn Cheng Yunfeng Fang Meiyun Feng Jianming Fu Haixia Gao Hong Han Yue He Aili Hou Ming Hu Yu Huang Ruibin Huang Wenrong Jing Zhicheng Kong Peiyan Liang Aibin Liang Meiying Liu Daihong Liu Junling Liu Lin Liu Xiaowei Ma Liangming Mei Heng Ni Heyu Niu Ting Peng Jun Qiao Jianlin Ren Jinhai Song Yongping Tang Liang V Tong Tong Wang Shaoyuan Wang Xin Wang Zhao Wei Hui Wu Depei Wu Guangsheng Xu Caigang Xu Xue Xu Yajing Yang Linhua Yang Renchi Yang Tonghua Yin Chenghong Yu Li Zhang Guangsen Zhang Lei Zhang Liansheng Zhang Xi Zhao Weili Zhao Yongqiang Zhou Daobin Zhou Hu Zhou Zeping Zhu Tienan Wang Jianliu Huang Xiaojun | 2022 | Chinese Medical Journal2022，，8： | 2 |
| 19 | Generation of isogenic single and multiplex gene knockout mice by base editing-induced STOP显示文摘Although CRISPR/Cas9 has been widely used to generate knockout mice, two major limitations remain:the founders usually carry a mixture of genotypes, and mosaicism harboring multiple genotypes.Therefore, it takes a long time to get homozygous mutants. Recently developed base editing(BE) system,which introduces C-to-T conversion without double strand DNA cleavage, has been used to introduce artificial stop codons(i-STOP) to prematurely terminate translation, providing a cleaner strategy for genome engineering. Using this strategy, we generated CD160 KO and VISTA/CD160 double KO mice by microinjection of a single sg RNA targeting CD160 and a mixture of sg RNAs targeting VISTA and CD160,respectively. The BE system induced STOP efficiently in mouse embryos and consequently in founder mice without detectable off-target. Most interestingly, the majority of the mutants harbor same genetic modifications, indicating we generated isogenic single and multiplex gene mutant mice by BE-induced STOP. We also obtained homozygous mutant mouse in F1 mice, demonstrating the accelerated strategy in generating animal models. | Guang Yang Tianyu Zhu Zongyang Lu Guanglei Li Hao Zhang Songjie Feng Yajing Liu Jianan Li Yu Zhang Jia Chen Xuejiang Guo Xingxu Huang | 2018 | Science Bulletin2018，63，17： | 2 |
| 20 | Indiscriminate ssDNA cleavage activity of CRISPR-Cas12a induces no detectable off-target effects in mouse embryos显示文摘Dear Editor,Newly discovered characteristics like'collateral effect'or trans-cleavage in CRISPR-Cas13 and CRISPR-Cas12 systems have enabled their usage in nucleic acid detection(Gootenberg et al.2017,2018;Chen et al.2018).The collateral RNA cleavage of Cas13a has been reported to be harmful for cell development(Wang et al.2019;Buchman et al.2020).As a representative gene editor of CRISPR-Cas12 system,CRISPR-Cas12a(Cpf1)holds great potential for therapeutic applications in the future(Zetsche et al.2015;Koo et al.2018;Campa et al.2019). | Yu Wei Yingsi Zhou Yajing Liu Wenqin Ying Ruiming Lv Qimeng Zhao Haibo Zhou Erwei Zuo Yidi Sun Hui Yang Changyang Zhou | 2021 | Protein & Cell2021，12，9： | 2 |
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