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| 1 | Impact of mechanical circulatory support and immunomodulation therapy on outcome of patients with fulminant myocarditis: Chinese registry of fulminant myocarditis显示文摘Dear Editor,Patients presenting with acute myocarditis and sudden hemodynamic instability (termed fulminant myocarditis [FM]) still have a high mortality and need for heart transplantation, up to 28% at 60 days.1,2,3 Recent scientific statements and expert opinion consensus suggests early use of temporary mechanical circulatory supports (t-MCS).3,4 Specifically, Chinese scientific statement proposed an extensive use of t-MCS combined with immunoregulatory therapy (IT),4 although formal trials are lacking. We present a multicenter, retrospective study to compare the outcome of patients who were treated with t-MCS and IT vs. patients who didn’t receive these treatments. We included patients with the diagnosis of FM based on the presence of viral prodromal signs/symptoms followed by acute onset of severe heart failure (HF) without other relevant differential diagnosis or pre-existing cardiac disorders. Patients who received both t-MCS and IT during hospitalization were classified as t-MCS+IT group. | Ning Zhou Yuhua Zhao Jiangang Jiang Lan Shen Junming Li Jing Wan Xueping Ma Jing Zhang Enrico Ammirati Dao Wen Wang | 2021 | Signal Transduction and Targeted Therapy2021，6，11： | 14 |
| 2 | The fabrication of biomimetic biphasic CAN-PAC hydrogel with a seamless interfacial layer applied in osteochondral defect repair显示文摘Cartilage tissue engineering based on biomimetic scaffolds has become a rapidly developing strategy for repairing cartilage defects. In this study, a biphasic CAN-PAC hydrogel for osteochondral defect(OCD)regeneration was fabricated based on the density difference between the two layers via a thermally reactive,rapid cross-linking method. The upper hydrogel was cross-linked by CSMA and NIPAm, and the lower hydrogel was composed of PECDA, AAm and PEGDA. The interface between the two layers was first grafted by the physical cross-linking of calcium gluconate and alginate, followed by the chemical cross-linking of the carbon-carbon double bonds in the other components. The pore sizes of the upper and lower hydrogels were ~ 187.4 and ~ 112.6 μm, respectively. The moduli of the upper and lower hydrogels were ~ 0.065 and~ 0.261 MPa. This prepared bilayer hydrogel exhibited the characteristics of mimetic composition, mimetic structure and mimetic stiffness, which provided a microenvironment for sustaining cell attachment and viability. Meanwhile, the biodegradability and biocompatibility of the CAN-PAC hydrogel were examined in vivo. Furthermore, an osteochondral defect model was developed in rabbits, and the bilayer hydrogels were implanted into the defect. The regenerated tissues in the bilayer hydrogel group exhibited new translucent cartilage and repaired subchondral bone, indicating that the hydrogel can enhance the repair of osteochondral defects. | Jinfeng Liao Taoran Tian Sirong Shi Xueping Xie Quanquan Ma Guo Li Yunfeng Lin | 2017 | Bone Research2017，5，2： | 10 |
| 3 | Temporal and Spatial Distribution of the Late Devonian (Famennian) Strata in the Northwestern Border of the Junggar Basin, Xinjiang, Northwestern China显示文摘The base of the Saerba Member(Mbr) of the Hongguleleng Formation(Fm.) probably lies in the Famennian Palmatolepis crepida Zone;the Longkou Mbr is probably a sedimentary wedge that thins out northwards;the Duguer Mbr has an approximate age from the upper part of the Pa.marginifera Zone or the Lower Pa.rugosa trachytera Zone through the top of the Pa.perlobata postera Zone;the Wulan Mbr has an age approximately corresponding to the whole Pa.gracilis expansa Zone.In the Bulongguoer section,the Lower Mbr of the Hongguleleng Fm.corresponds to Famennian Pa.crepida Zone through Pa.marginifera Zone;the Middle Mbr probably ranges from the Pa.r.trachytera Zone through the Pa.g.expansa Zone.The basal Namu Mbr of the Heishantou Fm.is probably the product during and after the Hangenberg Event in the upper part of the Siphonodella praesulcata Zone,which is still within the Devonian.In this context,the underlying Chasi Mbr may approximately correspond to the lower part of the S.praesulcata Zone(before the Hangenberg Event).Lateral distribution of strata indicates that the Upper Devonian in the Gennaren and Saerba areas each constitutes a structure of syncline,which differs from the previous recognition of a monocline structure. | MA Xueping ZHANG Meiqiong ZONG Pu ZHANG Yubo Lü Dan | 2017 | Acta Geologica Sinica(English Edition)2017，91，4： | 9 |
| 4 | Middle and Late Devonian brachiopod assemblages,sea level change and paleogeography of Hunan,China显示文摘Givetian through middle Famennian brachiopods of Hunan are used to define five biozones,comprising,in ascending order,the Stringocephalus Abundance Zone,the Ambocoeliid-Leiorhynchid Assemblage Zone,the Cyrtospiriferid Assemblage Zone,the Yunnanellina-Sinospirifer Assemblage Zone,and the Yunnanella-Hunanospirifer Assemblage Zone.They correspond approximately to the Po.hemiansatus Zone to Po.varcus Zone,S.hermanni Zone to Pa.punctata Zone,Pa.hassi Zone to Pa.linguiformis Zone,Pa.triangularis Zone to Upper Pa.crepida Zone,and Uppermost Pa.crepida Zone to Pa.marginifera Zone,respectively.Intensified rifting activity in South China during the Devonian began in the middle of the Lower Po.varcus Zone(a level marked by the first appearance of Po.rhenanus),which is consistent with the Lower-Middle Givetian substage boundary suggested by the International Subcommission on Devonian Stratigraphy(SDS).This assumed that rifting activity in the Lower Po.varcus Zone was signified by the deposition of the Baqi Formation in Guangxi and was marked largely by the extensive deposition of the Qiziqiao Formation in central Hunan,which probably led to an opposite pattern of relative sea level change-rising in deeper water trough areas and lowering in carbonate platform areas.The previously recognized S.hermanni Zone sea level rise in platform margin areas of Guangxi can be identified not only in the trough areas but also in platform areas,which probably indicates an overall marine transgression at this time,rather than tectonically induced sea level change.Similarly,this level coincides with the Middle-Upper Givetian substage boundary suggested by the SDS.Although the sea level rise may have occurred in the deeper water trough areas of South China towards the end of the Givetian,platformal areas experienced uplifting at various degrees,especially in the central and northern regions of Hunan where Early Frasnian deposits were apparently absent in many places.Similarly,the end-Frasnian regression led to exposure of many places and absence of early Famennian deposits,which probably accounts for the restricted distribution of the Yunnanellina brachiopod fauna. | XuePing Ma Pu Zong | 2010 | Science China Earth Sciences2010，53，12： | 7 |
| 5 | A Middle-Upper Devonian Boundary Section in the Open Platform,Platform Margin Facies of Guilin,South China显示文摘The Caiziyan Middle and Upper Devonian boundary section is located approximately 30 km northeast of Guilin.It hosts relatively abundant benthic and common-rare pelagic fossils,including brachiopods,corals,tentaculites,and conodonts,which may serve as a better suitable section for pelagic and neritic stratigraphic correlation.In this section,10'standard'conodont zones are recognized across the Givetian-Frasnian boundary,including,in descending order,the Lower hassi Zone,punctata Zone,transitans Zone,the Upper falsiovalis Zone,the Lower falsiovalis Zone,disparilis Zone,the Upper hermanni-cristatus Zone,the Lower hermanni-cristatus Zone,the Upper varcus Zone, and the Middle varcus Zone,all of which are defined by the first occurrence of their defining conodont species.The Middle-Upper Devonian(Givetian-Frasnian) boundary is defined by the first occurrence of Ancyrodella pristina in accordance with the Global Stratotype Section and Point(GSSP),which is assigned at 6.2m above the base of bed 19 in the Caiziyan section. | LI Hua MA Xueping WEI Longming | 2009 | Acta Geologica Sinica(English Edition)2009，83，3： | 7 |
| 6 | Atrypid Brachiopods from the Upper Devonian Wangchengpo Formation(Frasnian)of southern Guizhou,China—Extinction Patterns in the Frasnian of South China显示文摘The Wangchengpo Frasnian section of Dushan County contains two atrypid brachiopod assemblages. The lower is characterized by the Atryparia (Costatrypa) dushanensis fauna that appears at the base of the Hejiazhai Member approximately in the falsiovalis to transitans conodont zones; the upper is characterized by the Radiatrypa yangi fauna, which appears in the Lujiazhai Member approximately correspondent with the hassi to Upper rhenana conodont zones. Atrypid brachiopods, together with other brachiopods from the Dushan section show that the Hejiazhai Member is of Frasnian age. Preliminary analyses of Frasnian atrypid brachiopods from sections of South China indicate that there are nine genera and subgenera including Atryparia (Costatrypa), Kyrtatrypa, Spinatrypa, Isospinatrypa, Spinatrypina, Iowatrypa, Desquamatia (Desquamatia), Desquamatia (Seratrypa), and Radiatrypa. Atrypid species diversity did not change much through the Frasnian. On a regional scale in South China, most atrypid species went extinct prior to the Frasnian/Famennian boundary. At any specific locality or section, these atrypids became extinct about 20–40 m below the Frasnian/Famennian (F/F) boundary, within the linguiformis conodont Zone, marking this as the major extinction level. Three new atrypid species are described: Atryparia (Costatrypa) dushanensis, Iowatrypa pseudobodini, and Radiatrypa yangi. | MA Xueping Paul COPPER SUN Yuanlin LIAO Weihua | 2005 | Acta Geologica Sinica(English Edition)2005，79，4： | 7 |
| 7 | Synthesis of TiO_(2) nanotubes film and its light scattering property显示文摘TiO2 nanotubes with diameters of 10 nm and lengths up to 600 nm were fabricated by directly using com-mercial TiO2 powders P25 as the precursors via sonica-tion-hydrothermal combination approach. TiO2 nanotubes were characterized by means of X-ray powder diffractometer (XRD), scanning electron microscope (SEM), selected area electron diffraction pattern (SAED) and transmission elec-tron microscope (TEM). The light scattering property of film electrodes modified with TiO2 nanotubes was studied and revealed that TiO2 nanotubes can be used as the light scat-tering centers to increase the light absorption in dye- sensi-tized solar cells. The TiO2 nanotubes film electrodes mixed with 10% small nanoparticles TiO2 had both strong light scattering property and fine mechanical characteristics, and this kind of electrodes can be used as electrodes in improving the conversion efficiencies of dye-sensitized solar cells. | MA Yutao LIN Yuan XIAO Xurui LI Xueping ZHOU Xiaowen | 2005 | Chinese Science Bulletin2005，50，18： | 6 |
| 8 | Nanocrystalline TiO_(2) thin film electrodes prepared by common pressure hydrothermal method at low temperature显示文摘Nanocrystalline TiO2 thin film electrodes have been prepared from mixed pastes of tetrabutyl titanate and nanocrystalline TiO2 particles by common pressure hydro- thermal method at low temperature. The tetrabutyl titanate was hydrolyzed and crystallized into anatase TiO2 to inter- connect nanocrystalline TiO2 particles and adhere them to conductive substrates, obtaining highly porous and me- chanically stable TiO2 nanocrystalline film. The conversion efficiencies of the dye-sensitized solar cells based on prepared electrodes on conductive glass substrates and flexible sub- strates were 4.8% and 1.9% under illumination of 100 mW/cm2, respectively. | LI Chengyu LIN Yuan LI Xueping WANG Zhengping MA Yutao ZHOU Xiaowen FENG Shujing XIAO Xurui | 2005 | Chinese Science Bulletin2005，50，14： | 5 |
| 9 | Nucleic acids and analogs for bone regeneration显示文摘With the incidence of different bone diseases increasing, effective therapies are needed that coordinate a combination of various technologies and biological materials. Bone tissue engineering has also been considered as a promising strategy to repair various bone defects. Therefore, different biological materials that can promote stem cell proliferation, migration, and osteoblastic differentiation to accelerate bone tissue regeneration and repair have also become the focus of research in multiple fields. Stem cell therapy, biomaterial scaffolds, and biological growth factors have shown potential for bone tissue engineering; however, off-target effects and cytotoxicity have limited their clinical use. The application of nucleic acids(deoxyribonucleic acid or ribonucleic acid)and nucleic acid analogs(peptide nucleic acids or locked nucleic acids), which are designed based on foreign genes or with special structures, can be taken up by target cells to exert different effects such as modulating protein expression, replacing a missing gene, or targeting specific gens or proteins. Due to some drawbacks, nucleic acids and nucleic acid analogs are combined with various delivery systems to exert enhanced effects, but current studies of these molecules have not yet satisfied clinical requirements. In-depth studies of nucleic acid or nucleic acid analog delivery systems have been performed, with a particular focus on bone tissue regeneration and repair. In this review, we mainly introduce delivery systems for nucleic acids and nucleic acid analogs and their applications in bone repair and regeneration. At the same time, the application of conventional scaffold materials for the delivery of nucleic acids and nucleic acid analogs is also discussed. | Yuxin Zhang Wenjuan Ma Yuxi Zhan Chenchen Mao Xiaoru Shao Xueping Xie Xiawei Wei Yunfeng Lin | 2018 | Bone Research2018，6，4： | 3 |
| 10 | Multi?targeted Antisense Oligonucleotide Delivery by a Framework Nucleic Acid for Inhibiting Biofilm Formation and Virulence显示文摘Biofilm formation is responsible for numerous chronic infections and represents a serious health challenge.Bacteria and the extracellular polysaccharides(EPS)cause biofilms to become adherent,toxic,resistant to antibiotics,and ultimately difficult to remove.Inhibition of EPS synthesis can prevent the formation of bacterial biofilms,reduce their robustness,and promote removal.Here,we have developed a framework nucleic acid delivery system with a tetrahedral configuration.It can easily access bacterial cells and functions by delivering antisense oligonucleotides that target specific genes.We designed antisense oligonucleotide sequences with multiple targets based on conserved regions of the VicK protein-binding site.Once delivered to bacterial cells,they significantly decreased EPS synthesis and biofilm thickness.Compared to existing approaches,this system is highly efficacious because it simultaneously reduces the expression of all targeted genes(gtfBCD,gbpB,ftf).We demonstrate a novel nucleic acid-based nanomaterial with multi-targeted inhibition that has great potential for the treatment of chronic infections caused by biofilms. | Yuxin Zhang Xueping Xie Wenjuan Ma Yuxi Zhan Chenchen Mao Xiaoru Shao Yunfeng Lin | 2020 | Nano-Micro Letters2020，12，6： | 3 |
| 11 | Accessible chromatin regions and their functional interrelations with gene transcription and epigenetic modifications in sorghum genome显示文摘Accessible chromatin regions(ACRs)provide physical scaffolds to recruit transcriptional co-regulators and displace their nearby nucleosomes in multiple plant species.Characterization of ACRs and investigation of their biological effects in Sorghum bicolor has lagged behind.Regulation of gene expression relies on the transcriptional co-regulators that are recruited to ACRs to affect epigenomic modifications of surrounding nucleosomes.In this study,we employed transposase-accessible chromatin sequencing to identify ACRs and decipher how the presence of ACRs affects gene expression and epigenetic signatures in the Sorghum genome.As a result,21077 ACRs,which are mapped to 22.9%of genes and 2.7%of repeats,were identified.The profiling of ACRs on gene structures reveals a narrow and sharp peak around the transcription start site,with relatively weak and broad signals covering the entire gene body and an explicit but wide peak from the transcription termination site to its downstream regions.We discovered that the correlations between gene expression levels and profiled ACR densities are dependent on the positions of ACRs.The occurrence of genic ACRs cumulatively enhances the transcriptional activity of intergenic ACR-associated genes.In addition,an intricate crosstalk among ACRs,gene expression,and epigenetic marks has been unveiled by integrating multiple-omics analyses of whole-genome bisulfite sequencing,6mA immunoprecipitation followed by sequencing,RNA sequencing,chromatin immunoprecipitation sequencing,and DNase I hypersensitive sites sequencing datasets.Our study provides a genome-wide landscape of ACRs in sorghum,decrypts their interrelations with various epigenetic marks,and sheds new light on their roles in transcriptional regulation. | Chao Zhou Zhu Yuan Xueping Ma Huilan Yang Ping Wang Lanlan Zheng Yonghong Zhang Xiaoyun Liu | 2021 | Plant Communications2021，2，1： | 2 |
| 12 | The biological applications of DNA nanomaterials:current challenges and future directions显示文摘DNA,a genetic material,has been employed in different scientific directions for various biological applications as driven by DNA nanotechnology in the past decades,including tissue regeneration,disease prevention,inflammation inhibition,bioimaging,biosensing,diagnosis,antitumor drug delivery,and therapeutics.With the rapid progress in DNA nanotechnology,multitudinous DNA nanomaterials have been designed with different shape and size based on the classic Watson–Crick base-pairing for molecular self-assembly.Some DNA materials could functionally change cell biological behaviors,such as cell migration,cell proliferation,cell differentiation,autophagy,and anti-inflammatory effects.Some single-stranded DNAs(ssDNAs)or RNAs with secondary structures via self-pairing,named aptamer,possess the ability of targeting,which are selected by systematic evolution of ligands by exponential enrichment(SELEX)and applied for tumor targeted diagnosis and treatment.Some DNA nanomaterials with three-dimensional(3D)nanostructures and stable structures are investigated as drug carrier systems to delivery multiple antitumor medicine or gene therapeutic agents.While the functional DNA nanostructures have promoted the development of the DNA nanotechnology with innovative designs and preparation strategies,and also proved with great potential in the biological and medical use,there is still a long way to go for the eventual application of DNA materials in real life.Here in this review,we conducted a comprehensive survey of the structural development history of various DNA nanomaterials,introduced the principles of different DNA nanomaterials,summarized their biological applications in different fields,and discussed the current challenges and further directions that could help to achieve their applications in the future. | Wenjuan Ma Yuxi Zhan Yuxin Zhang Chenchen Mao Xueping Xie Yunfeng Lin | 2021 | Signal Transduction and Targeted Therapy2021，6，11： | 2 |
| 13 | Devonian integrative stratigraphy and timescale of China显示文摘The Global Boundary Stratotype Sections and Points(GSSPs) for the bases of all seven international Devonian stages have been formally defined and ratified by IUGS till 1996, and nowadays, the main tasks for Devonian stratigraphers include further subdivision of these standard stages, strictly constrained absolute ages for the boundaries, and precise neritic-pelagic and marine-terrestrial correlations using multidisciplinary stratigraphy methods. Establishment of high-resolution Devonian integrative stratigraphy framework and timescale of China would play an important role in improving regional and international correlation, facilitating the recognition of important stratigraphic levels in different paleogeographic settings, and understanding the evolution pattern of biota, paleoclimate and paleoenvironment during this critical interval. Based on well-studied bio-and chronostratigraphy of Devonian in South China and adjacent areas, in combination with recent achievements in carbon isotope stratigraphy, event stratigraphy and radioactive isotope ages, this paper briefly summarize the research history and current status of Devonian chronostratigraphy of China, and for the first time introduce Devonian integrative stratigraphy framework of China.Up to date, few studies have been conducted on the astronomical cyclostratigraphy and high-resolution radioactive isotope dating in Devonian of China, which should be our main focuses in the near future. | Wenkun QIE Xueping MA Honghe XU Li QIAO Kun LIANG Wen GUO Junjun SONG Bo CHEN Jianfeng LU | 2019 | Science China Earth Sciences2019，62，1： | 2 |
| 14 | Effects of thyme(Thymus vulgaris L.)addition on the volatile compounds of mutton broth during boiling显示文摘To investigate the effects of thyme(Thymus vulgaris L.)addition on the flavor modification of mutton broth during boiling,three mutton-broth samples with various thyme contents 0.06%(S1),0.30%(S2),and1.50%(S3)were analyzed and compared,without thyme as control(0.00%,S0).The meaty,fatty,pastoral flavor and spicy were chosen as sensory attributes to evaluate the flavor of the mutton-broth samples.Sensory results were significantly different(P<0.001),with S2 having the optimum overall acceptability.A total of 99 volatile compounds were identified by gas chromatography-mass spectrometry,among which19 compounds were considered as the odor-active compounds according to their odor activity values.Significant changes(P<0.05)appeared in most volatiles in S0 with thyme addition,especially aldehydes.Free fatty acids(FFAs)were also identified,and all of them significantly increased with increased thyme(P<0.05).Correlation analysis of odor-active compounds,FFAs,and sensory attributes through partial least squares regression indicated the important volatiles and FFAs remarkably contributed to the mutton broth samples,and further confirmed that the 0.30%of thyme may be a desirable addition amount for the sensory characteristics of mutton broth. | Shasha Qi Ping Zhan Honglei Tian Peng Wang Xueping Ma Kaixuan Li | 2022 | Food Science and Human Wellness2022，11，2： | 2 |
| 15 | Fair Efficiency Comparisons of Decoy-State Quantum Key Distribution Protocols显示文摘Taking the schemes based on different formulas of secure key rate as examples, we give a fair comparison between typical protocols under Universal composable(UC) security frame. Through analyzing the relationship of security parameters in post-processing stage and final secure key rate, we achieve the unified quantification between protocols based on Gottesman-Lo-L¨utkenhausPreskill(GLLP) formula and the ones under UC security.Based on the above research, the impact of different sending lengths and secure parameters on secure key rate is investigated. Besides, we analyze the conditions of fair comparison, and for the first time we give a fair comparison between the protocols based on GLLP formula and smooth entropy. By typical efficiency comparisons, we summarize the way for improving secure key rate in the light intensity choice and the single bit error rate estimation method. | LI Hongxin GAO Ming YAN Xueping YAN Bao HAN Yu SHAN Ling MA Zhi | 2018 | Chinese Journal of Electronics2018，27，2： | 1 |
| 16 | Recent development and application of several high-efficiency surface heat exchangers for energy conversion and utilization显示文摘 | Qiuwang Wang Min Zeng Ting Ma Xueping Du Jianfeng Yang | 2014 | Applied Energy2014，，： | 1 |
| 17 | Interception, degradation and contributions of terrestrial organic carbon obtained from lignin analysis in Wujiang River, southwest China显示文摘Understanding the fate of terrestrial organic carbon(OC)in a cascade impoundment system is critical for recognizing the role of carbon sink for reservoirs.Surface sediments collected from eight cascade reservoirs across the Wujiang River,southwestern China,were analyzed for elemental and stable carbon isotopic(δ^13C)composition,and lignin phenols(∑8 andΛ8)to investigate the spatial distribution,contribution,origin and degradation of sedimentary terrestrial OC.The values of total organic carbon(TOC)and∑8 exhibited a remarkable reduction along the upstream-downstream transect suggesting the trapping effect of cascade-damming.A relatively broad range ofδ^13C(-26.61 to-25.54%,95%CI)and C/N(6.80-18.20)indicated mixed allochthonous/autochthonous OC sources in surface sediments.The quantitative simulation indicates that the OC of the sediments mainly was derived from terrestrial organic matter.Soilderived OC rather than C_(3)vascular plant-derived OC makes a major contribution to sedimentary terrestrial OC in reservoirs on karst terrain.As evidenced by lignin compositions andδ^13C,the predominant vascular plant origins of terrestrial OC along the Wujiang River are non-woody angiosperm C_(3)plants.The aged reservoirs showed a trend of increasing contribution of autochthonous OC,which potentially weaken the role of carbon sink for reservoirs.The relationship between runoff inputs,watershed area/water surface area ratios,and water residence time and Λ8 were explored,indicating the natural and anthropogenic influences on terrestrial OC remains very complex in a cascade-damming river. | Xin Lin Yujie Wang Jinhua Zhang Ming Yang Xueping Chen Fushun Wang Jing Ma | 2021 | Acta Geochimica2021，40，6： | 1 |
| 18 | Effect of Sulfonation Degree on Performance of Proton Exchange Membranes for Direct Methanol Fuel Cells显示文摘 | XIANG Zheng ZHAO Xueping GE Junjie MA Shuhua ZHANG Yuwei NA Hui | 2016 | Chemical Research in Chinese Universities2016，32，2： | 1 |
| 19 | Early Enteral Nutrition within 24 Hours or between 24 and 72 Hours for Acute Pan- creatitis :Evidence Based on 12 RCTs 显示文摘 | Xueping Li Fengbo Ma Kezhi Jia | 2014 | Med Sci Monit2014，，20： | 1 |
| 20 | Construction of a high-density mutant population of Chinese cabbage facilitates the genetic dissection of agronomic traits显示文摘Chinese cabbage(Brassica rapa ssp.pekinensis)is an economically important vegetable crop throughout the world,especially in Asia.High-quality genome sequences are available for Chinese cabbage,but gene functional studies remain challenging.To promote functional genomic studies of Chinese cabbage,we generated an ethyl methane sulfonate(EMS)mutant population of~8000 M_(2) plants using the double haploid inbred line A03 as the parent.The genome of A03 was sequenced and used as a reference for high-throughput functional characterization of gene mutations at the whole-genome level.A total of 300 M_(2) to M_(5) EMS mutants were phenotypically screened and then sequenced,revealing 750629 SNPs and 46272 InDel mutations that cover 98.27%of all predicted genes in the A03 genome.A forward-genetics approach was successfully used to identify two genes with chloroplast-related functions that are responsible for the yellow leaf mutant trait.A reverse-genetics approach was also used to identify associations between mutations in five genes of the glucosinolate biosynthetic pathway and variations in glucosinolate content of the mutant plants.In addition,we built the Chinese cabbage EMS mutation database(CCEMD,www.bioinformaticslab.cn/EMSmutation/home)to increase the usability of this mutant population resource.In summary,we performed large-scale screening of a heading Chinese cabbage EMS mutant collection at the phenotypic and genotypic levels,which will facilitate gene mining of Chinese cabbage and might also be useful for the study of other Brassica crops. | Xiaoxue Sun Xing Li Yin Lu Shan Wang Xiaomeng Zhang Kang Zhang Xiangjie Su Mengyang Liu Daling Feng Shuangxia Luo Aixia Gu Yu Fu Xueping Chen Shuxin Xuan Yanhua Wang Donghui Xu Shumin Chen Wei Ma Shuxing Shen Feng Cheng Jianjun Zhao | 2022 | Molecular Plant2022，15，5： | 1 |
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