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| 1 | Generation and characterization of stable pig pregastrulation epiblast stem cell lines显示文摘Pig epiblast-derived pluripotent stem cells are considered to have great potential and broad prospects for human therapeutic model developme nt and livestock breeding.Despite on going attempts since the 1990s,no stably defined pig epiblast-derived stem cell line has bee n established.Here,guided by in sights from a large-scale sin gle-cell tran scriptome an alysis of pig embryos from embryonic day(E)0 to E14,specifically,the tracing of pluripotency changes during epiblast development,we developed an in vitro culture medium for establishing and maintaining stable pluripotent stem cell lines from pig E10 pregastrulation epiblasts(pgEpiSCs).Enabled by chemical inhibition of WNT-related signaling in combination with growth factors in the FGF/ERK,JAK/STAT3,and Activin/Nodal pathways,pgEpiSCs maintain their pluripotency transcriptome features,similar to those of E10 epiblast cells,and normal karyotypes after more than 240 passages and have the potential to differentiate into three germ layers.Strikingly,ultradeep in situ Hi-C analysis revealed functional impacts of chromatin 3D-spatial associations on the transcriptional regulation of pluripote ncy marker genes in pgEpiSCs.I n practice,we con firmed that pgEpiSCs readily tolerate at least three rounds of successive gene editi ng and gene rated cloned gen e-edited live piglets.Our findings deliver on the long-a nticipated promise of pig pluripote nt stem cells and open new avenues for biological research,animal husbandry,and regenerative biomedicine. | Minglei Zhi Jinying Zhang Qianzi Tang Dawei Yu Shuai Gao Dengfeng Gao Pengliang Liu Jianxiong Guo Tang Hai Jie Gao Suying Cao Zimo Zhao Chongyang Li Xiaogang Weng Mengnan He Tianzhi Chen Yingjie Wang Keren Long Deling Jiao Guanglei Li Jiaman Zhang Yan Liu Yu Lin Daxin Pang Qianqian Zhu Naixin Chen Jingjing Huang Xinze Chen Yixuan Yao Jingcang Yang Zicong Xie Xianya Huang Mengxin Liu Ran Zhang Qiuyan Li Yiliang Miao Jianhui Tian Xingxu Huang Hongsheng Ouyang Bofeng Liu Wei Xie Qi Zhou Zhonghua Liu Caihong Zheng Mingzhou Li Jianyong Han | 2022 | Cell Research2022，32，4： | 3 |
| 2 | Flexible Triboelectric Nanogenerator from Micro-nano Structured Polydimethylsiloxane显示文摘 | XIAO Xinze Lü Chao WANG Gong XU Ying WANG Jiping YANG Hai | 2015 | Chemical Research in Chinese Universities2015，31，3： | 3 |
| 3 | Adaptive spraying decision system for plant protection unmanned aerial vehicle based on reinforcement learning显示文摘To solve the problem of lacking scientific guidance in aerial pesticide application,this study introduced an adaptive spraying decision system(ASDS)for Unmanned Aerial Vehicle(UAV)spraying to guide the operators of plant protection UAVs to set reasonable spraying parameters under complicated environment.The minimum applied volume rate,proper spraying velocity,spraying height,and initial droplet size were recommended by the ASDS.The key factor of the decision system is the decision model of reinforcement learning based on the actor-critic neural network.In specific,the field experimental data were used to train the critic and actor networks,which made the model adaptive to optimize the output of spraying parameters.Compared with the conventional spraying parameters,the spraying parameters recommended by the ASDS had a positive impact on wheat parcels.The decision results of the ASDS showed that the spraying volume rate was lower in the blocks with a small leaf area index.In addition,the spraying volume rate for the whole parcel was reduced by 14%.After UAV spraying,the uniformity of the droplet deposition in the ASDS parcel was better than that in the conventional parcel.Moreover,the penetrability of the droplets and the control efficacy for the brown wheat mite Petrobia latens(Muller)were similar in the two parcels.The ASDS can recommend the optimal spraying parameters to minimize pesticide application. | Ziyuan Hao Xinze Li Chao Meng Wei Yang Minzan Li | 2022 | International Journal of Agricultural and Biological Engineering2022，15，4： | 1 |
| 4 | Implantable intracortical microelectrodes: reviewing the present with a focus on the future显示文摘Implantable intracortical microelectrodes can record a neuron’s rapidly changing action potentials(spikes).In vivo neural activity recording methods often have either high temporal or spatial resolution,but not both.There is an increasing need to record more neurons over a longer duration in vivo.However,there remain many challenges to overcome before achieving long-term,stable,high-quality recordings and realizing comprehensive,accurate brain activity analysis.Based on the vision of an idealized implantable microelectrode device,the performance requirements for microelectrodes are divided into four aspects,including recording quality,recording stability,recording throughput,and multifunctionality,which are presented in order of importance.The challenges and current possible solutions for implantable microelectrodes are given from the perspective of each aspect.The current developments in microelectrode technology are analyzed and summarized. | Yang Wang Xinze Yang Xiwen Zhang Yijun Wang Weihua Pei | 2023 | Microsystems & Nanoengineering2023，9，1： | 1 |
| 5 | The Arabidopsis A4subfamily of Lectin receptor kinases negatively regulatesabscisic acid response in seed germination 显示文摘 | XinZ Wang A Yang G | 2009 | PlantPhysiol2009，149，43： | 1 |
| 6 | LDL receptor-related protein 1 (LRP1),a novel target for opening the blood-labyrinth barrier(BLB)显示文摘Inner ear disorders are a cluster of diseases that cause hearing loss in more than 1.5 billion people worldwide.However,the presence of the blood-labyrinth barrier(BLB)on the surface of the inner ear capillaries greatly hinders the effectiveness of systemic drugs for prevention and intervention due to the low permeability,which restricts the entry of most drug compounds from the bloodstream into the inner ear tissue.Here,we report the finding of a novel receptor,low-density lipoprotein receptor-related protein 1(LRP1),that is expressed on the BLB,as a potential target for shuttling therapeutics across this barrier.As a proof-ofconcept,we developed an LRP1-binding peptide,IETP2,and covalently conjugated a series of model small-molecule compounds to it,including potential drugs and imaging agents.All compounds were successfully delivered into the inner ear and inner ear lymph,indicating that targeting the receptor LRP1 is a promising strategy to enhance the permeability of the BLB.The discovery of the receptor LRP1 will illuminate developing strategies for crossing the BLB and for improving systemic drug delivery for inner ear disorders. | Xi Shi Zihao Wang Wei Ren Long Chen Cong Xu Menghua Li Shiyong Fan Yuru Xu Mengbing Chen Fanjun Zheng Wenyuan Zhang Xinbo Zhou Yue Zhang Shiwei Qiu Liyuan Wu Peng Zhou Xinze Lv Tianyu Cui Yuehua Qiao Hui Zhao Weiwei Guo Wei Chen Song Li Wu Zhong Jian Lin Shiming Yang | 2022 | Signal Transduction and Targeted Therapy2022，7，7： | 0 |
| 7 | Flexible multichannel electrodes for acute recording in nonhuman primates显示文摘Flexible electrodes have demonstrated better biocompatibility than rigid electrodes in relieving tissue encapsulation and long-term recording.Nonhuman primates are closer to humans in their brains’structural and functional properties,thus making them more suitable than rodents as animal models for potential clinical usage.However,the application of flexible electrodes on nonhuman primates has rarely been reported.In the present study,a flexible multichannel electrode array for nonhuman primates was developed and implemented for extracellular recording in behaving monkeys.To minimize the window of durotomy for reducing possible risks,a guide-tube-compatible implantation solution was designed to deliver the flexible electrodes through the dura into the cortex.The proposed structure for inserting flexible electrodes was characterized ex vivo and validated in vivo.Furthermore,acute recording of multichannel flexible electrodes for the primates was performed.The results showed that the flexible electrodes and implantation method used in this study meet the needs of extracellular recording in nonhuman primates.Task-related neuronal activities with a high signal-to-noise ratio of spikes demonstrated that our whole device is currently a minimally invasive and clinically viable approach for extracellular recording. | Yang Wang Qifan Wang Ruichen Zheng Xinxiu Xu Xinze Yang Qiang Gui Xiaowei Yang Yijun Wang He Cui Weihua Pei | 2023 | Microsystems & Nanoengineering2023，9，4： | 0 |
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