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| 1 | Efficient genome editing in plants using a CRISPR/Cas system显示文摘 | Zhengyan Feng Botao Zhang Wona Ding Xiaodong Liu Dong-Lei Yang Pengliang Wei Fengqiu Cao Shihua Zhu Feng Zhang Yanfei Mao Jian-Kang Zhu | 2013 | Cell Research2013，23，10： | 211 |
| 2 | Factors predicting sensory and motor recovery after the repair of upper limb peripheral nerve injuries显示文摘OBJECTIVE: To investigate the factors associated with sensory and motor recovery after the repair of upper limb peripheral nerve injuries. DATA SOURCES: The online PubMed database was searched for English articles describing outcomes after the repair of median, ulnar, radial, and digital nerve injuries in humans with a publication date between 1 January 1990 and 16 February 2011. STUDY SELECTION: The following types of article were selected:(1) clinical trials describing the repair of median, ulnar, radial, and digital nerve injuries published in English; and(2) studies that reported sufficient patient information, including age, mechanism of injury, nerve injured, injury location, defect length, repair time, repair method, and repair materials. SPSS 13.0 software was used to perform univariate and multivariate logistic regression analyses and to investigate the patient and intervention factors associated with outcomes. MAIN OUTCOME MEASURES: Sensory function was assessed using the Mackinnon-Dellon scale and motor function was assessed using the manual muscle test. Satisfactory motor recovery was defined as grade M4 or M5, and satisfactory sensory recovery was defined as grade S3+ or S4.RESULTS: Seventy-one articles were included in this study. Univariate and multivariate logistic regression analyses showed that repair time, repair materials, and nerve injured were independent predictors of outcome after the repair of nerve injuries(P < 0.05), and that the nerve injured was the main factor affecting the rate of good to excellent recovery. CONCLUSION: Predictors of outcome after the repair of peripheral nerve injuries include age, gender, repair time, repair materials, nerve injured, defect length, and duration of follow-up. | Bo He Zhaowei Zhu Qingtang Zhu Xiang Zhou Canbin Zheng Pengliang Li Shuang Zhu Xiaolin Liu Jiakai Zhu | 2014 | Neural Regeneration Research2014，9，6： | 12 |
| 3 | Generation and characterization of stable pig pregastrulation epiblast stem cell lines显示文摘Pig epiblast-derived pluripotent stem cells are considered to have great potential and broad prospects for human therapeutic model developme nt and livestock breeding.Despite on going attempts since the 1990s,no stably defined pig epiblast-derived stem cell line has bee n established.Here,guided by in sights from a large-scale sin gle-cell tran scriptome an alysis of pig embryos from embryonic day(E)0 to E14,specifically,the tracing of pluripotency changes during epiblast development,we developed an in vitro culture medium for establishing and maintaining stable pluripotent stem cell lines from pig E10 pregastrulation epiblasts(pgEpiSCs).Enabled by chemical inhibition of WNT-related signaling in combination with growth factors in the FGF/ERK,JAK/STAT3,and Activin/Nodal pathways,pgEpiSCs maintain their pluripotency transcriptome features,similar to those of E10 epiblast cells,and normal karyotypes after more than 240 passages and have the potential to differentiate into three germ layers.Strikingly,ultradeep in situ Hi-C analysis revealed functional impacts of chromatin 3D-spatial associations on the transcriptional regulation of pluripote ncy marker genes in pgEpiSCs.I n practice,we con firmed that pgEpiSCs readily tolerate at least three rounds of successive gene editi ng and gene rated cloned gen e-edited live piglets.Our findings deliver on the long-a nticipated promise of pig pluripote nt stem cells and open new avenues for biological research,animal husbandry,and regenerative biomedicine. | Minglei Zhi Jinying Zhang Qianzi Tang Dawei Yu Shuai Gao Dengfeng Gao Pengliang Liu Jianxiong Guo Tang Hai Jie Gao Suying Cao Zimo Zhao Chongyang Li Xiaogang Weng Mengnan He Tianzhi Chen Yingjie Wang Keren Long Deling Jiao Guanglei Li Jiaman Zhang Yan Liu Yu Lin Daxin Pang Qianqian Zhu Naixin Chen Jingjing Huang Xinze Chen Yixuan Yao Jingcang Yang Zicong Xie Xianya Huang Mengxin Liu Ran Zhang Qiuyan Li Yiliang Miao Jianhui Tian Xingxu Huang Hongsheng Ouyang Bofeng Liu Wei Xie Qi Zhou Zhonghua Liu Caihong Zheng Mingzhou Li Jianyong Han | 2022 | Cell Research2022，32，4： | 3 |
| 4 | Chromosomal-level genome of macadamia(Macadamia integrifolia)显示文摘Macadamia from the family Proteaceae is a plant native to Australia and has long been favoured by people for its crispy and high nutritional and medicinal value.Here,the genome of GUIRE 1(GR1),a highly heterozygous superior cultivar of macadamia nut,was sequenced and assembled using nanopore sequencing,and a 807-Mb genome(contig N50,1.9 Mb;scaffold N50,54.70 Mb)and 14 chromosomes were obtained.A total of 453 Mb(about 55.95%)repetitive sequences and 37,657 protein-coding genes were obtained by gene annotation and homologous protein comparison.Proteaceae diverged from Nelumbonaceae nearly 115.37 million years ago and from Rubiaceae about 140 million years ago.A genome-wide duplication(WGD)event occurred in macadamia 41 million years ago based on the WGD analysis.The functional enrichment analysis of M.integrifolia-specific gene families revealed their roles in signal transduction,protein phosphorylation,protein binding,and defense response.Here,a highly heterozygous genome of M.integrifolia was unlocked to provide a database for breeding and molecular mechanism research. | Chengcai Xia Sirong Jiang Qiujin Tan Wenquan Wang Long Zhao Chenji Zhang Yuting Bao Qi Liu Jianjia Xiao Ke Deng Miaohua He Pengliang An Wenlin Wang Meiling Zou Zhiqiang Xia | 2022 | Tropical Plants2022，1，1： | 3 |
| 5 | Reorganization of 3D genome architecture across wild boar and Bama pig adipose tissues显示文摘Background:A growing body of evidence has revealed that the mammalian genome is organized into hierarchical layers that are closely correlated with and may even be causally linked with variations in gene expression.Recent studies have characterized chromatin organization in various porcine tissues and cell types and compared them among species and during the early development of pigs.However,how chromatin organization differs among pig breeds is poorly understood.Results:In this study,we investigated the 3D genome organization and performed transcriptome characterization of two adipose depots(upper layer of backfat[ULB]and greater omentum[GOM])in wild boars and Bama pigs;the latter is a typical indigenous pig in China.We found that over 95%of the A/B compartments and topologically associating domains(TADs)are stable between wild boars and Bama pigs.In contrast,more than 70%of promoterenhancer interactions(PEIs)are dynamic and widespread,involving over a thousand genes.Alterations in chromatin structure are associated with changes in the expression of genes that are involved in widespread biological functions such as basic cellular functions,endocrine function,energy metabolism and the immune response.Approximately 95%and 97%of the genes associated with reorganized A/B compartments and PEIs in the two pig breeds differed between GOM and ULB,respectively.Conclusions:We reported 3D genome organization in adipose depots from different pig breeds.In a comparison of Bama pigs and wild boar,large-scale compartments and TADs were mostly conserved,while fine-scale PEIs were extensively reorganized.The chromatin architecture in these two pig breeds was reorganized in an adipose depotspecific manner.These results contribute to determining the regulatory mechanism of phenotypic differences between Bama pigs and wild boar. | Jiaman Zhang Pengliang Liu Mengnan He Yujie Wang Hua Kui Long Jin Diyan Li Mingzhou Li | 2022 | Journal of Animal Science and Biotechnology2022，13，4： | 2 |
| 6 | Inhibition of spore germination of Ulva pertusa by the marine bacterium Pseudoalteromonas haloplanktis CI4显示文摘The effect of the bacterial strain CI4 on the germination of spores from the green alga Ulva pertusa was assayed and it was found that the bacterial biofilm and cell-free supernatant strongly inhibited spore germination. In attempts to define the chemical nature of the antifouling substance in the supernatant of CI4,the culture supernatants were tested for activity after heat treatment,enzymatic treatments,size fractionation,and separation into aqueous and organic fractions. Results suggest that this bacterium produces an extracellular component with specific activity toward algal spores that was heat-sensitive and between 3 and 10 kDa in molecular size. The exposure of the organic phase fraction to spores showed inhibitive effect on spore germination. Pronase and carboxypeptidase y did not significantly affect the activity of inhibitory component,suggesting that the component was not a protein or a peptide. The bacterium CI4 was identified as Pseudoalteromonas haloplanktis based on the phenotypic characters and 16S rRNA gene analysis. | MA Yuexin LIU Pengliang ZHANG Yongsheng CAO Shanmao LI Dantong CHEN Wei | 2010 | Acta Oceanologica Sinica2010，29，1： | 1 |
| 7 | Experimental and Numerical Study on the Gas Explosion in Urban Regulator Station显示文摘Regulator station is an important part in the urban gas transmission and distribution system.Once gas explosion occurs,the real explosion process and consequences of methane gas explosion in the regulator station were not revealed systematically.In this study,a full-scale experiment was carried out to simulate the regulator station explosion process,and some numerical simulations with a commercial CFD software called FLACS were conducted to analyze the effect of ignition and vent conditions on the blast overpressure and flame propagation.The experimental results demonstrated that the peak overpressure increased as the distance from the vent increased within a certain distance.And the maximum overpressure appeared 3 m away from the door,which was about 36.6 kPa.It was found that the pressure-time rising curves obtained from the simulation are basically the same as the ones from the experiment,however,the time of reaching the peak pressure was much shorter.The numerical simulation results show that the peak overpressures show an increase trend as the ignition height decreased and the vent relief pressure increased.It indicates that the damage and peak overpressure of gas explosion could be well predicted by FLACS in different styles of regulator station.In addition,the results help us to understand the internal mechanism and development process of gas explosion better.It also offers technical support for the safety protection of the urban regulator station. | Zhenyi Liu Yuanyuan Ma Yuan Ren Mingzhi Li Pengliang Li Song Wan | 2020 | Journal of Beijing Institute of Technology2020，29，2： | 1 |
| 8 | Biomimetic ultrathin pepsomes for photo-controllable catalysis显示文摘Artificial vesicles for mimicking the unique structures and functions of natural organelles represent a promising scientific object in biomimicry.However,the development of the stimuli-responsive and ultrathin vesicles assembled from sequence-defined biomimetic polymers for controllable applications is still a significant challenge.Herein,we report the self-assembly of azobenzene-based amphiphilic alternating peptoids to generate photo-responsive and ultrathin peptoid vesicle(pepsomes)with an average diameter of∼180 nm.Both cryo-transmission electron microscopy(TEM)and dissipative particle dynamics simulation proved that the vesicular membrane was the ultrathin bilayer structure around∼1.6 nm.The photo-responsive ability of pepsomes was demonstrated by the reversible size changes upon the alternative irradiation with ultraviolet(UV)and visible lights,which was attributable to the photoisomerization virtue of azobenzene moiety.As a proof-of-concept,the photo-controllable catalytic action of gold nanoparticles-decorated pepsomes was evaluated toward the borohydride-mediated reduction from 4-nitrophenol to 4-aminophenol.Photo-controllable reversible and recyclable catalytic activity was effectively modulated using the alternative irradiation with UV and visible lights for five cycles.Our work provides a simple strategy to prepare stimuli-responsive and ultrathin vesicles for potential application on nanocatalysis. | Guiping Peng Haibao Jin Fan Liu Xiaoyan Yang Pengliang Sui Shaoliang Lin | 2022 | Science China Chemistry2022，65，12： | 1 |
| 9 | Repairing of exit-hole in friction-stir-spot welded joints for 2024-T4 aluminum alloy by resistance welding显示文摘The exit-hole in friction stir spot welded(FSSWed) 2024-T4 aluminum alloy joints was successfully repaired by using a three-phase secondary rectification resistance spot welding machine, which is termed as filling exit-hole based on resistance welding(FEBRW). The filling dynamic behavior of force was recorded by a device monitoring. Optical microscope(OM), electron backscatter diffraction(EBSD), and tensile shear tests and finite element modelling were conducted to investigate the repairing stages and bonding mechanisms of the repaired joints in detail. Results showed that exit-hole was completely filled and repaired experiencing three stages. Metallurgical bonding was achieved between plug and exit-hole wall in two forms, including melting bonding in the middle of the joints and partial diffusion bonding on both the upper and bottom of the joints. The highest tensile shear strength of the repaired joints was 7.43 kN, which was 36.3% higher than that of the as welded joints. Resistance welding paves an efficient way to repair the exit-hole in FSSWed joints. | Lipeng Deng Pengliang Niu Liming Ke Jinhe Liu Jidong Kang | 2023 | International Journal of Minerals,Metallurgy and Materials2023，30，4： | 0 |
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