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| 1 | Effect of Yiqihuoxue prescription on myocardial energy metabolism after myocardial infarction via cross talk of liver kinase B1-depen-dent Notch1 and adenosine 5'-monophosphate-activated protein kinase显示文摘OBJECTIVE: To investigate the effect of Yiqihuoxue prescription(YQHX) from Traditional Chinese Medicine(TCM) on myocardial glucose and lipid metabolism after myocardial infarction via the cross talk between the liver kinase B1(LKB1)-dependent Notch1 and adenosine 5'-monophosphate(AMP)-activated protein kinase(AMPK). YQHX was prepared with substances with properties that benefit, to activate blood circulation based on the TCM theory.METHODS: Animal models of myocardial infarction were established by ligating Sprague Dawley rats' left anterior descending coronary arteries. The animals were randomly divided into a myocardial infarction(MI) group, a YQHX group, a perindopril group, a γ-secretase inhibitor, Notch signal inhibitor(DAPT) group, a DAPT+YQHX group and a sham group. The related drugs were administered on the second day after operation, and changes in the relevant indexes were examined on weeks 1 and 4.Changes in cardiac structure and function were examined by echocardiography. The glucose and free fatty acids(FFA) were examined by ELISA. The expression of Notch, LKB1 and AMPK m RNA was examined by a real-time fluorescence quantitative method. The expression of glucose transporter 4(GLUT4), and the expression of total acetyl-Co A carboxylase(ACC) and its phosphorylation were examined by western blotting.RESULTS: Compared with the sham group, the expression of Notch, LKB1 and AMPK m RNA in the MI group was lower. Compared with the MI group, the expression of these m RNAs in the YQHX and perindopril groups was higher, and their expression in the DAPT group was lower. At all time points, the protein expression of GLUT4 and p ACC decreased in the MI group. On week 1, the expression of p ACC protein was higher. In the DAPT group, the expression of p ACC protein decreased. Compared with the YQHX group, the expression of p ACC protein in the DAPT + YQHX group was lower. On week 4,compared with the MI group, the expression of GLUT4 protein in the YQHX group and the perindo-pril group was higher. The expression of GLUT4 protein in the DAPT group decreased. Compared with the YQHX group, the expression of GLUT4 protein in the DAPT+YQHX group was lower. There was no significant difference in the expression of ACC protein between the groups.CONCLUSION: YQHX promoted cross talk between the LKB1-dependent Notch1 and AMPK in myocardial tissue after myocardial infarction. Furthermore,it regulated the glucose and lipid metabolism of cardiomyocytes at different time points, thereby ameliorating the cardiac energy metabolism via different mechanisms and protecting the heart. | Wu Jiangong Chen Xi Guo Shuwen Liu Wenchen Zhang Lu Li Fanghe Wu Jiani Huang Xiaolou Cai Qian Tan Xiaobo Wang Hui | 2017 | Journal of Traditional Chinese Medicine2017，37，3： | 7 |
| 2 | Exosomes rewire the cartilage microenvironment in osteoarthritis:from intercellular communication to therapeutic strategies显示文摘Osteoarthritis(OA)is a prevalent degenerative joint disease characterized by cartilage loss and accounts for a major source of pain and disability worldwide.However,effective strategies for cartilage repair are lacking,and patients with advanced OA usually need joint replacement.Better comprehending OA pathogenesis may lead to transformative therapeutics.Recently studies have reported that exosomes act as a new means of cell-to-cell communication by delivering multiple bioactive molecules to create a particular microenvironment that tunes cartilage behavior.Specifically,exosome cargos,such as noncoding RNAs(ncRNAs)and proteins,play a crucial role in OA progression by regulating the proliferation,apoptosis,autophagy,and inflammatory response of joint cells,rendering them promising candidates for OA monitoring and treatment.This review systematically summarizes the current insight regarding the biogenesis and function of exosomes and their potential as therapeutic tools targeting cell-to-cell communication in OA,suggesting new realms to improve OA management. | Yuangang Wu Jiao Li Yi Zeng Wenchen Pu Xiaoyu Mu Kaibo Sun Yong Peng Bin Shen | 2022 | International Journal of Oral Science2022，14，3： | 4 |
| 3 | Periostin mediates epithelial-mesenchymal transition through the MAPK/ERK pathway in hepatoblastoma显示文摘Objective: The aim of the present study was to analyze the prognostic factors in patients with hepatoblastoma(HB) in our single center and to evaluate periostin(POSTN) expression in HB and its association with clinicopathological variables. In addition, the underlying mechanism of how POSTN promotes HB progression was discussed.Methods: POSTN expression was investigated in HB tumors by immunohistochemistry(IHC), immunofluorescence(IF) and Western blot(WB). The association among POSTN expression, clinicopathological features and overall survival(OS) was also evaluated. The migration and adhesion ability of HB cells were measured using chemotaxis and cell-matrix adhesion assays,respectively. Epithelial-mesenchymal transition(EMT)-associated markers and activation of the ERK pathway were detected by WB.Results: HB patients had poor prognosis which displayed lymph node metastasis, vascular invasion, POSTN and vimentin expression. POSTN expression was also associated with lymph node metastasis. Furthermore, overexpressed POSTN promoted migration and the adhesive ability of HB cells in vitro. In addition, we demonstrated that POSTN activated the MAPK/ERK pathway, upregulated the expression of Snail and decreased the expression of OVOL2. Finally, POSTN promoted the expression of EMT-associated markers.Conclusions: POSTN might modulate EMT via the ERK signaling pathway, thereby promoting cellular migration and invasion.Our study also suggests that POSTN may serve as a therapeutic biomarker in HB patients. | Lu Chen Xiangdong Tian Wenchen Gong Bo Sun Guangtao Li Dongming Liu Piao Guo Yuchao He Ziye Chen Yuren Xia Tianqiang Song Hua Guo | 2019 | Cancer Biology & Medicine2019，16，1： | 3 |
| 4 | Long non-coding RNA LncKdm2b regulates cortical neuronal differentiation by cis-activating Kdm2b显示文摘The mechanisms underlying spatial and temporal control of cortical neurogenesis of the brain are largely elusive.Long non-coding RNAs(lncRNAs)have emerged as essential cell fate regulators.Here we found LncKdm2b(also known as Kancr),a lncRNA divergently transcribed from a bidirectional promoter of Kdm2b,is transiently expressed during early differentiation of cortical projection neurons.Interestingly,Kdm2b’s transcription is positively regulated in cis by LncKdm2b,which has intrinsic-activating function and facilitates a permissive chromatin environment at the Kdm2b’s promoter by associating with hnRNPAB.Lineage tracing experiments and phenotypic analyses indicated LncKdm2b and Kdm2b are crucial in proper differentiation and migration of cortical projection neurons.These observations unveiled a lncRNA-dependent machinery in regulating cortical neuronal differentiation. | Wei Li Wenchen Shen Bo Zhang Kuan Tian Yamu Li Lili Mu Zhiyuan Luo Xiaoling Zhong Xudong Wu Ying Liu Yan Zhou | 2020 | Protein & Cell2020，11，3： | 2 |
| 5 | NUMB enhances Notch signaling by repressing ubiquitination of NOTCH1 intracellular domain显示文摘The release and nuclear translocation of the intracellular domain of Notch receptor(NICD)is the prerequisite for Notch signaling-mediated transcriptional activation.NICD is subjected to various posttranslational modifications including ubiquitination.Here,we surprisingly found that NUMB proteins stabilize the intracellular domain of NOTCH1 receptor(N1ICD)by regulating the ubiquitin–proteasome machinery,which is independent of NUMB’s role in modulating endocytosis.BAP1,a deubiquitinating enzyme(DUB),was further identified as a positive N1ICD regulator,and NUMB facilitates the association between N1ICD and BAP1 to stabilize N1ICD.Intriguingly,BAP1 stabilizes N1ICD independent of its DUB activity but relying on the BRCA1-inhibiting function.BAP1 strengthens Notch signaling and maintains stem-like properties of cortical neural progenitor cells.Thus,NUMB enhances Notch signaling by regulating the ubiquitinating activity of the BAP1–BRCA1 complex. | Zhiyuan Luo Lili Mu Yue Zheng Wenchen Shen Jiali Li Lichao Xu Bo Zhong Ying Liu Yan Zhou | 2020 | Journal of Molecular Cell Biology2020，12，5： | 2 |
| 6 | In Situ Monitoring the Potassium‑Ion Storage Enhancement in Iron Selenide with Ether‑Based Electrolyte显示文摘As one of the promising anode materials,iron selenide has received much attention for potassium-ion batteries(KIBs).Nevertheless,volume expansion and sluggish kinetics of iron selenide result in the poor reversibility and stability during potassiation–depotassiation process.In this work,we develop iron selenide composite matching ether-based electrolyte for KIBs,which presents a reversible specific capacity of 356 mAh g^(−1) at 200 mA g^(−1) after 75 cycles.According to the measurement of mechanical properties,it is found that iron selenide composite also exhibits robust and elastic solid electrolyte interphase layer in ether-based electrolyte,contributing to the improvement in reversibility and stability for KIBs.To further investigate the electrochemical enhancement mechanism of ether-based electrolyte in KIBs,we also utilize in situ visualization technique to monitor the potassiation–depotassiation process.For comparison,iron selenide composite matching carbonate-based electrolyte presents vast morphology change during potassiation–depotassiation process.When changing to ether-based electrolyte,a few minor morphology changes can be observed.This phenomenon indicates an occurrence of homogeneous electrochemical reaction in ether-based electrolyte,which results in a stable performance for potassium-ion(K-ion)storage.We believe that our work will provide a new perspective to visually monitor the potassium-ion storage process and guide the improvement in electrode material performance. | Xiaodan Li Jinliang Li Wenchen Zhuo Zhibin Li Liang Ma Zhong Ji Likun Pan Wenjie Mai | 2021 | Nano-Micro Letters2021，13，11： | 2 |
| 7 | Insights on the mechanism of Na-ion storage in expanded graphite anode显示文摘Currently,Na-ion battery(NIB) has become one of the most potential alternatives for Li-ion batteries due to the safety and low cost.As a promising anode for Na-ion storage,expanded graphite has attracted considerable attention.However,the sodiation-desodiation process is still unclear.In our work,we obtain expanded graphite through slight modified Hummer's method and subsequent thermal treatment,which exhibits excellent cycling stability.Even at a high current density of 1 A g^(-1),our expanded graphite still remains a high reversible capacity of 100 mA h g^(-1) after 2600 cycles.Furthermore,we also investigate the electrochemical mechanism of our expanded graphite for Na-ion storage by operando Raman technique,which illuminate the electrochemical reaction during different sodiation-desodiation processes. | Xiaodan Li Zhibin Liu Jinliang Li Hang Lei Wenchen Zhuo Wei Qin Xiang Cai Kwun Nam Hui Likun Pan Wenjie Mai | 2021 | Journal of Energy Chemistry2021，30，2： | 1 |
| 8 | Time evolution of the degree distribution of model A of random attachment growing networks 显示文摘 | He Wenchen Feng Lei Li Lingyun | 2007 | PhysicaA2007，384，2： | 1 |
| 9 | Time evlution of the degree distribution of model A of random attachment growing networks显示文摘 | He Wenchen Feng Lei Li Lingyun | 2007 | Phys A2007，384，： | 1 |
| 10 | Scaled radon-wigner transform imaging and scaling of ma- neuvering target 显示文摘 | Li Wenchen Wang Xuesong Wang Guoyu | 2010 | IEEE Transactions on Aero- space and Electronic Systems2010，46，4： | 1 |
| 11 | Salvianolic acid B attenuates cyclooxygenase‐2 expression in vitro in LPS‐treated human aortic smooth muscle cells and in vivo in the apolipoprotein‐E‐deficient mouse aorta显示文摘 | Yuh‐LienChen Cing‐SiangHu Feng‐YenLin Yung‐HsiangChen Li‐MinSheu Hung‐HaiKu Ming‐ShiShiao Jaw‐WenChen Shing‐JongLin | 2006 | J Cell Biochem2006，，3： | 1 |
| 12 | Rotational parameters estimation of maneuvering target in ISAR imaging显示文摘The rotational parameters estimation of maneuvering target is the key of cross-range scaling of ISAR (inverse synthetic aperture radar), which can be used in the target feature extraction. The cross-range signal model of rotating target with fixed acceleration is presented and the weighted linear least squares estimation of rotational parameters with fixed velocity or acceleration is proposed via the relationship of cross-range FM (frequency modulation) parameter, scatterers coordinates and rotational parameters. The FM parameter is calculated via RWT (Radon-Wigner transform). The ISAR imaging and cross-range scaling based on scaled RWT imaging method are implemented after obtaining rotational parameters. The rotational parameters estimation and cross-range scaling are validated by the ISAR processing of experimental radar data, and the method presents good application foreground to the ISAR imaging and scaling of maneuvering target. | Wenchen Li Jin Liu Xuesong Wang Shunping Xiao Guoyu Wang | 2010 | Journal of Systems Engineering and Electronics2010，21，1： | 1 |
| 13 | Auxiliary diagnostic system for ADHD in children based on AI technology显示文摘Traditional diagnosis of attention deficit hyperactivity disorder(ADHD)in children is primarily through a questionnaire filled out by parents/teachers and clinical observations by doctors.It is inefficient and heavily depends on the doctor’s level of experience.In this paper,we integrate artificial intelligence(AI)technology into a software-hardware coordinated system to make ADHD diagnosis more efficient.Together with the intelligent analysis module,the camera group will collect the eye focus,facial expression,3D body posture,and other children’s information during the completion of the functional test.Then,a multi-modal deep learning model is proposed to classify abnormal behavior fragments of children from the captured videos.In combination with other system modules,standardized diagnostic reports can be automatically generated,including test results,abnormal behavior analysis,diagnostic aid conclusions,and treatment recommendations.This system has participated in clinical diagnosis in Department of Psychology,The Children’s Hospital,Zhejiang University School of Medicine,and has been accepted and praised by doctors and patients. | Yanyi ZHANG Ming KONG Tianqi ZHAO Wenchen HONG Di XIE Chunmao WANG Rongwang YANG Rong LI Qiang ZHU | 2021 | Frontiers of Information Technology & Electronic Engineering2021，22，3： | 1 |
| 14 | Time evolution of the degree distribution of model A of random attachment growing networks 显示文摘 | He Wenchen Feng Lei Li Lingyun | 2007 | PhysicaA2007，384，2： | 1 |
| 15 | Corrigendum to“Injectable muscle-adhesive antioxidant conductive photothermal bioactive nanomatrix for efficiently promoting full-thickness skeletal muscle regeneration”[Bioact Mater,6(2021)1605-1617]显示文摘The authors regret that the fluorescent image for FPC-7day in Figure 5A was reproduced incorrectly,although which did not affect the conclusion of this work. | Li Zhou Juan Ge Min Wang Wei Cheng Wenchen Ji Bo Lei | 2021 | Bioactive Materials2021，6，12： | 1 |
| 16 | Injectable muscle-adhesive antioxidant conductive photothermal bioactive nanomatrix for efficiently promoting full-thickness skeletal muscle regeneration显示文摘The completed skeletal muscle regeneration resulted from severe injury and muscle-related disease is still a challenge.Here,we developed an injectable muscle-adhesive antioxidant conductive bioactive photothermo-responsive nanomatrix for regulating the myogenic differentiation and promoting the skeletal muscle regeneration in vivo.The multifunctional nanomatrix was composed of polypyrrole@polydopamine(PPy@PDA,342±5.6 nm)nanoparticles-crosslinked Pluronic F-127(F127)-polycitrate matrix(FPCP).The FPCP nanomatrix demonstrated inherent multifunctional properties including excellent photothermo-responsive and shear-thinning behavior,muscle-adhesive feature,injectable ability,electronic conductivity(0.48±0.03 S/m)and antioxidant activity and photothermal function.The FPCP nanomatrix displayed better photothermal performance with near-infrared irradiation,which could provide the photo-controlled release of protein(91%±2.6%of BSA was released after irradiated 3 times).Additionally,FPCP nanomatrix could significantly enhance the cell proliferation and myogenic differentiation of mouse myoblast cells(C2C12)by promoting the expressions of myogenic genes(MyoD and MyoG)and myosin heavy chain(MHC)protein with negligible cytotoxicity.Based on the multifunctional properties,FPCP nanomatrix efficiently promoted the full-thickness skeletal muscle repair and regeneration in vivo,through stimulating the angiogenesis and myotube formation.This study firstly indicated the vital role of multifunctional PPy@PDA nanoparticles in regulating myogenic differentiation and skeletal muscle regeneration.This work also suggests that rational design of bioactive matrix with multifunctional feature would greatly enhance the development of regenerative medicine. | Li Zhou Juan Ge Min Wang Mi Chen Wei Cheng Wenchen Ji Bo Lei | 2021 | Bioactive Materials2021，6，6： | 0 |
| 17 | A retrospective analysis of mature T-and NK-cell lymphomas显示文摘Mature T-and natural killer(NK)-cell lymphomas are heterogeneous groups of malignant lymphoid neoplasms arising from T and NK cells. The incidence of mature T-and NK-cell lymphomas is 2.1 per 100,000 people, according to a US report~1. | Junlei Jia Xiaohui Wang Zheng Song Shen Meng Yue Fei Jingwei Yu Xia Liu Xue Han Lanfang Li Lihua Qiu Zhengzi Qiani Shiyong Zhou Wenchen Gong Bin Meng Xiubao Ren Xianhuo Wang Huilai Zhang | 2024 | Cancer Biology & Medicine2024，21，3： | 0 |
| 18 | THE CONTROL, DRIVE AND DISPLAY CIRCUITS ANDDEVICES IN OPTO-ELECTRONIC 2-D PROGRAMMABLE NEURAL NETWORK显示文摘In an optoelectronic 2-D programmable neural network system, optical data need to be transferred and feedback with high speed. This paper presents the design and implementation of the interface circuit and its software. | Li Wenchen Zhang Zhiguang(Department of Electronic Science, Nankai University, Tianjin 300071)Zhang Yanxin (Institute of Modern Optics, Nankai University, Tianjin 300071) | 1997 | Journal of Electronics(China)1997，14，1： | 0 |
| 19 | Protein aggregation in association with delayed neuronal death in rat model of brain ischemia显示文摘To investigate the relationship between protein aggregation and delayed neuronal death,we adopted rat models of 20 min ischemia.Brain ischemia was produced using the 2-vessel occlusion(2VO)model in rats Light microscopy,transmission electronic microscopy and Western blot analysis were performed for morphological analysis of neurons,and protein detection.The results showed delayed neuronal death took place at 72 h after ischemia-reperfusion,protein aggregates formed at 4 h after reperfusion and reached the peak at 24 h after reper-fusion,and Western blot analysis was consistent with transmission electronic microscopy.We conclude that protein aggregation is one of the important factors leading to delayed neuronal death. | Pengfei GE Tianfei LUO Shuanglin FU Wenchen LI Chonghao WANG Chuibing ZHOU Yinan LUO | 2008 | Frontiers of Medicine2008，2，1： | 0 |
| 20 | Cysteine-113 covalency inspires the development of Pin1 inhibitor显示文摘Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1(Pin1)controls the functional switch of phosphoproteins and plays an oncogenic role in human cancer,but the discovery of effective Pin1 inhibitors remains challenging.A recent study by Pinch et al.developed a novel Pin1 inhibitor by endowing molecule with covalent reactivity toward Pin1 cysteine-113,1 providing new insights into the design of anticancer Pin1 inhibitors. | Wenchen Pu Jiao Li Yong Peng | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 0 |
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