|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 西藏色季拉山林线冷杉种群结构与动态显示文摘急尖长苞冷杉(Abies georgei var.smithii)是西藏东南部地区高山林线森林群落的主要建群树种,主要分布在色季拉山海拔3600~4400m区域,并成为阴坡高山林线的优势树种。通过对色季拉山林线群落交错区域的定位调查,分析了急尖长苞冷杉的个体生长、种群结构与动态以及林线特征。分析结果表明:(1)西藏色季拉山海拔4320m处为森林郁闭上限,该区域存在两种类型的高山林线,阳坡为渐变型林线,林线树种为方枝柏(Sabina saltuaria);阴坡为急变型林线,林线树种为急尖长苞冷杉。阳坡与阴坡林线分布海拔上限分别为4570m和4390m,阳坡高于阴坡180m;阳坡与阴坡林线群落交错区垂直宽度分别为250m和70m,阳坡比阴坡宽180m。(2)阴坡海拔3700~3800m属急尖长苞冷杉分布的最适范围,种群径级结构表现为典型的反'J'型,种群密度约380株/hm2;种群年龄结构表现为'金字塔'型,属于扩展型种群。(3)静态生命表和种群存活曲线反映了急尖长苞冷杉种群在形成初期的20a和生长发育的60~160a分别经历了强烈的环境筛选和竞争自疏,以及后期与环境变化相关的死亡波动,200a左右为急尖长苞冷杉的生理寿命,种群后期基本稳定,400a左右为极限寿命。 | 任青山 杨小林 崔国发 王景升 黄瑜 WEI Xiao hua LI Qinglin | 2007 | 生态学报2007，27，7： | 70 |
| 2 | Animal experiment and clinical study of effect of gamma-interferon on hepatic fibrosis显示文摘AIM To evaluate the antifibrotic effect ofdifferent doses of recombinant human Gamma-Interferon (IFN-γ) intwo rat models of hepaticfibrosis, and to observe its effect on moderatechronic hepatitis B virus fibrosis.METNODS Hepatic fibrosis was successfullyinduced in 150 and 196 rats by subcutaneousinjection of carbon tetrachloride (CCl4) andintraperitoneal injection of dimethylnitrosamine(DMN), respectively. Each of the two modeldose IFN-γ group (15 MU/kg per day, i.m. for 8group (1.67 MU/kg daily, i.m. for 8 weeks).Another group of 10 rats without any treatmentwas used as normal controls. At the end of theexperiment, semi-quantitative histopathologicalscores of inflammation and fibrosis, liver (αsmooth muscle actin (α-SMA) expression level,liver hydroxyl proline content and serumhyaluronic acid levels were compared. And 47medium chronic hepatitis B viral fibrosispatients were studied. They were given IFN-γtreatment, 100MU/day i.m. for the first threemonths and 100MU qod i.m. for the next sixmonths. Semi-quantitative pathological scoresof inflammation and fibrosis and serum hepaticfibrosis indices were compared within the 9months.RESULTS In animal experiment, thepathological fibrosis scores and liver hydroxylproline content were found to be significantlylower in rats treated with different doses of IFN-γ as compared with rats in fibrotic model groupinduced by either CCI4 or DMN, in a dose-dependent manner. For CCI4-induced model,pathological fibrosis scores in high, medium andIow doses IFN-γ groups were 5.10 ± 2.88, 7.70 ±3.53 and 8.00 ± 3.30, respectively, but the scorewas 14.60 ± 7.82 in fibrotic model group.Hydroxyl proline contents were 2.83 ± 1.18, 3.59± 1.22 and 4.80 ± 1.62, in the three IFN-γgroups, and 10.01 ± 3.23 in fibrotic model group.The difference was statistically significant(P<0.01). Similar results were found in DMN-induced model. Pathological fibrosis scoreswere 6.30±0.48, 8.10 ±2.72 and 8.30 ±2.58, inhigh, medium and Iow doses IFN-γ groups, and12.60 ± 3.57 in fibrotic model group. Hydroxylproline contents were 2.72 ± 0.58, 3.14 ± 0.71and 3.62 ± 1.02, in the three IFN-γ groups, and12.79 ± 1.54 in fibrotic model group. Thedifference was statistically significant(P<0.01). Serum hepatic fibrosis indicesdecreased significantly in the 47 patients afterIFN-γ treatment (HA: 433.38 ± 373.00 vs 281.57± 220.48; LN: 161.22± 41.02 vs 146.35 ± 44.67;PCⅢ: 192.59 ± 89.95 vs 156.98 ± 49.22; C-Ⅳ:156.30 ± 44.01 vs 139.14 ± 34.47) and thedifferences between the four indices weresignificant (P<0.05). Thirty-three patientsCONCLUSION All the three doses of IFN-γ areeffective in treating rat liver fibrosis induced byeither CCl4 or DMN, the higher the dose, thebetter the effect. And IFN-γ is effective forpatients with moderate chronic hepatitis B viralfibrosis. | Hong Lei Weng Wei Min Cai Rong Hua Liu Institute of Infectious Diseases, First Affiliated Hospital. Medical School. Zhejiang University, Hangzhou 310003, Zhejiang Province. China | 2001 | World Journal of Gastroenterology2001，7，1： | 53 |
| 3 | Inhibition of signal transducer and activator of transcription 3 expression by RNA interference suppresses invasion through inducing anoikis in human colon cancer cells显示文摘AIM:To investigate the roles and mechanism of signal transducer and activator of transcription 3 (STAT3) in invasion of human colon cancer cells by RNA interference. METHODS: Small interfering RNA (siRNA) targeting Signal transducer and activator of transcription 3 (STAT3) was transfected into HT29 colon cancer cells. STAT3 protein level and DNA-binding activity of STAT3 was evaluated by western blotting and electrophoretic mobility shift assay (EMSA), respectively. We studied the anchorage-independent growth using colony formation in soft agar, and invasion using the boyden chamber model, anoikis using DNA fragmentation assay and terminal deoxynucleotidyltransferase-mediated dUTP nick-end labeling (TUNEL), respectively. Western blot assay was used to observe the protein expression of Bcl-xL and survivin in colon cancer HT29 cells. RESULTS: RNA interference (RNAi) mediated by siRNA leads to suppression of STAT3 expression in colon cancer cell lines. Suppression of STAT3 expression by siRNA could inhibit anchorage-independent growth, and invasion ability, and induces anoikis in the colon cancer cell line HT29. It has been shown that knockdown of STAT3 expression by siRNA results in a reduction in expression of Bcl-xL and survivin in HT29 cells. CONCLUSION: These results suggest that STAT3 siRNA can inhibit the invasion ability of colon cancer cells through inducing anoikis, which antiapoptotic genes survivin and Bcl-xL contribute to regulation of anoikis.These studies indicate STAT3 siRNA could be a useful therapeutic tool for the treatment of colon cancer. | Yu Fan You-Li Zhang Ying Wu Wei Zhang Yin-Huan Wang Zhao-Ming Cheng Hua Li | 2008 | World Journal of Gastroenterology2008，14，3： | 51 |
| 4 | Hydrogen Sulfide Promotes Root Organogenesis in Ipomoea batatas, Salix matsudana and Glycine max显示文摘In this report, we demonstrate that sodium hydrosulfide (NaHS), a hydrogen sulfide (H2S) donor, promoted adventitious root formation mediated by auxin and nitric oxide (NO). Application of the H2S donor to seedling cuttings of sweet potato (Ipomoea batatas L.) promoted the number and length of adventitious roots in a dose-dependent manner. It was also verified that H2S or HS- rather than other sulfur-containing components derived from NaHS could be attributed to the stimulation of adventitious root formation. A rapid increase in endogenous H2S, indole acetic acid (IAA) and NO were sequentially observed in shoot tips of sweet potato seedlings treated with HaHS. Further investigation showed that H2S-mediated root formation was alleviated by N-1-naphthylphthalamic acid (NPA), an IAA transport inhibitor, and 2-(4-carboxyphenyl)- 4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO), an NO scavenger. Similar phenomena in H2S donor-dependent root organogenesis were observed in both excised willow (Salix matsudana var. tortuosa Vilm) shoots and soybean (Glycine max L.) seedlings. These results indicated that the process of H2S-induced adventitious root formation was likely mediated by IAA and NO, and that H2S acts upstream of IAA and NO signal transduction pathways. | Hua Zhang Jun Tang Xiao-Ping Liu Yun Wang Wei Yu Wei-Yan Peng Fang Fang Dai-Fu Ma Zhao-Jun Wei Lan-Ying Hu | 2009 | Journal of Integrative Plant Biology2009，51，12： | 58 |
| 5 | Comparing the Diagnostic Criteria for Gestational Diabetes Mellitus of World Health Organization 2013 with 1999 in Chinese Population显示文摘INTRODUCTION Gestational diabetes mellitus (GDM) was earlier defined as 'hyperglycemia first recognized during pregnancy' and has more recently been described by American Diabetes Association (ADA) (2012) as diabetes diagnosed during pregnancy that is not clearly overt diabetes.[1] The hyperglycemia and adverse pregnancy outcomes study (HAPO) demonstrated that the risk of adverse maternal,fetal,and neonatal outcomes continuously increase as a function of maternal glycemia at 24-28 weeks,even within ranges previously considered normal for pregnancy.[2] After reviewing the results of the HAPO study,many international diabetes study groups,including the International Association of Diabetes and Pregnancy Study Groups and ADA have adopted the 75 g oral glucose tolerance test (OGTT) at 24-28 weeks as a screening and diagnostic test and defined new cut-off values for GDM diagnosis.The Ministry of Health of China published the criteria for GDM,that is the same as the ADA criteria on July 1,2011.World Health Organization (WHO) has recommended the 75 g OGTT with different cut-offvalues as a diagnostic test since 1999,changed its recommendation on 2013.In Hongkong and Yunnan province in China,most hospital adopted the WHO 1999 criteria,nevertheless,there is no evidence of multicenter and large sample to show that the GDM population within the same ethnic group diagnosed by the new WHO criteria matches those diagnosed by the old one and consistency between the two criteria has not been reported.Thus,we conducted this study to analyze the two criteria in Chinese pregnant women. | Weiwei Zhu Huixia Yang Yumei Wei Zilian Wang Xuelan Li Hairong Wu Nan Li Meihua Zhang Xinghui Liu Hua Zhang Yunhui Wang Jianmin Niu Yujie Gan Liruo Zhong Yunfeng Wang Anil Kapur | 2015 | Chinese Medical Journal2015，，1： | 42 |
| 6 | Diagnosis and treatment recommendations for pediatric respiratory infection caused by the 2019 novel coronavirus显示文摘Since December 2019,an epidemic caused by novel coronavirus (2019-nCoV) infection has occurred unexpectedly in China.As of 8 pm,31 January 2020,more than 20 pediatric cases have been reported in China.Of these cases,ten patients were identified in Zhejiang Province,with an age of onset ranging from 112 days to 17 years.Following the latest National recommendations for diagnosis and treatment of pneumonia caused by 2019-nCo V (the 4th edition) and current status of clinical practice in Zhejiang Province,recommendations for the diagnosis and treatment of respiratory infection caused by 2019-nCoV for children were drafted by the National Clinical Research Center for Child Health,the National Children's Regional Medical Center,Children's Hospital,Zhejiang University School of Medicine to further standardize the protocol for diagnosis and treatment of respiratory infection in children caused by 2019-nCoV. | Zhi-Min Chen Jun-Fen Fu Qiang Shu Ying-Hu Chen Chun-Zhen Hua Fu-Bang Li Ru Lin Lan-Fang Tang Tian-Lin Wang Wei Wang Ying-Shuo Wang Wei-Ze Xu Zi-Hao Yang Sheng Ye Tian-Ming Yuan Chen-Mei Zhang Yuan-Yuan Zhang | 2020 | World Journal of Pediatrics2020，16，3： | 45 |
| 7 | The Efficacy and Safety of Wenxin Keli in Patients with Frequent Premature Ventricular Contractions: A Randomized, Double-blind, Placebo-controlled, Parallel-group, Multicenter Trial显示文摘 | Wei Hua Run-Lin Gao Bu-Chang Zhao Jing Wang Xu-Hua Chen Chi Cai Shu Zhang | 2015 | Chinese Medical Journal2015，，19： | 35 |
| 8 | The First Imported Case of Monkeypox in the Mainland of China—Chongqing Municipality,China,September 16,2022显示文摘Monkeypox is a zoonotic viral disease caused by the monkeypox virus(MPXV),and historically,all outbreaks have been linked to Africa;however,monkeypox has been posing an alarming challenge to the world in 2022(1)as approximately 60,000 cases have been reported in more than 100 nations and regions worldwide(2).Currently,many cases of monkeypox were identified in many nonendemic countries outside of Central and West Africa,and human-to-human transmission has occurred frequently,especially among men who have sex with men(MSM)presenting new clinical symptoms similar to syphilis and other sexually transmitted infections(3). | Hua Zhao Wenling Wang Li Zhao Sheng Ye Jingdong Song Roujian Lu Hua Zong Changcheng Wu Wei Huang Baoying Huang Yao Deng Ruhan A Wujuan Xie Li Qi Wenbo Xu Hua Ling Wenjie Tan | 2022 | China CDC weekly2022，4，38： | 35 |
| 9 | Microstructures and Mechanical Properties of Fe-Mn-(Al, Si) TRIP/TWIP Steels显示文摘The mechanical properties and microstructure of two low carbon high manganese steels with 23.8% (No.1) and 33% (No.2) (mass percent) of manganese were investigated. The results showed that No.1 steel possesses high strength and high plasticity, and No.2 steel has a relatively high strength and extraordinary plasticity. The No.1 steel exhibits both TRIP (transformation induced plasticity) and TWIP (twin induced plasticity) effects during the deformation; while only TWIP effect appeared under the same deformation condition for No.2 steel. The comparison between the microstructures and mechanical properties of two steels was made, and the strengthening mechanisms were also analyzed. | DING Hua TANG Zheng-You LI Wei WANG Mei SONG Dan | 2006 | Journal of Iron and Steel Research(International)2006，13，6： | 31 |
| 10 | The Relationship between Glucose Excursion and Cognitive Function in Aged Type 2 Diabetes Patients显示文摘Objective Evidence suggests that type 2 diabetes (T2DM) is associated with an increased risk of dementia and that glucose variability is an independent risk factor for diabetic complications.This study investigated the relationship between glucose excursion and cognitive function in aged T2DM patients.Methods A total of 248 aged T2DM patients wore a continuous glucose monitoring system (CGMS) for 3 days in order to evaluate glucose excursion,including mean amplitude of glycemic excursions (MAGE) and mean of daily difference (MODD).All subjects were evaluated with a number of accepted cognitive function tests,including themini‐mental status examination (MMSE).The relationship between MAGE and MODD and performance on these cognitive tests was assessed.Results The MAGE and MMSE score were negatively correlated,likewise with the correlation between MODD and MMSE.Liner multivariate regression analysis showed that MAGE and MODD were also negatively related to MMSE independent of age,sex,glycemic control,hypertension,smoking,or coronary heart disease history.Conclusion Glucose excursion is related to cognitive function in aged T2DM patients.Elevated glucose excursion decreased the MMSE score,which reflects general cognitive function.Thus,therapy aimed at controlling glucose excursion may be beneficial for maintaining cognitive function in aged T2DM patients. | ZHONG Yuan ZHANG Xiao Yan MIAO Ya ZHU Jie Hua YAN Hong WANG Bei Yun JIN Jun HU Ting Jun JIA Wei Ping | 2012 | Biomedical and Environmental Sciences2012，25，1： | 34 |
| 11 | Preliminary results pertaining to coseismic displacement and preseismic strain accumulation of the Lushan M_S7.0 earthquake,as reflected by GPS surveying显示文摘This paper presents the coseismic displacement and preseismic deformation fields of the Lushan MS7.0 earthquake that occurred on April 20,2013.The results are based on GPS observations along the Longmenshan fault and within its vicinity.The coseismic displacement and preseismic GPS results indicate that in the strain release of this earthquake,the thrust rupture is dominant and the laevorotation movement is secondary.Furthermore,we infer that any possible the rupture does not reach the earth’s surface,and the seismogenic fault is most likely one fault to the east of the Guanxian-Anxian fault.Some detailed results are obtainable.(1)The southern segment of the Longmenshan fault is locked preceding the Lushan earthquake.After the Wenchuan earthquake,the strain accumulation rate in the southeast direction accelerates in the epicenter of the Lushan earthquake,and the angle between the principal compressional strain and the seismogenic fault indicates that a sinistral deformation background in the direction of the seismogenic fault precedes the Lushan earthquake.Therefore,it is evident that the Wenchuan MS8.0 earthquake accelerated the pregnancy of the Lushan earthquake.(2)The coseismic displacements reflected by GPS data are mainly located in a region that is 230 km(NW direction)×100 km(SW direction),and coseismic displacements larger than 10 mm lie predominantly in a100-km region(NW direction).(3)On a large scale,the coseismic displacement shows thrust characteristics,but the associated values are remarkably small in the near field(within 70 km)of the earthquake fault.Meanwhile,the thrust movement in this70-km region does not correspond with the attenuation characteristics of the strain release,indicating that the rupture of this earthquake does not reach the earth’s surface.(4)The laevorotation movements are remarkable in the 50-km region,which is located in the hanging wall that is close to the earthquake fault,and the corresponding values in this case correlate with the attenuation characteristics of the strain release. | WU YanQiang JIANG ZaiSen WANG Min CHE Shi LIAO Hua LI Qiang LI Peng YANG YongLin XIANG HePing SHAO ZhiGang WANG WuXing WEI WenXin LIU XiaoXia | 2013 | Chinese Science Bulletin2013，58，28： | 33 |
| 12 | Effects of large dose of dexamethasone on inflammatory mediators and pancreatic cell apoptosis of rats with severe acute pancreatitis显示文摘AIM: To investigate the influence of high dose of dexamethasone on inflammatory mediators and apoptosis of rats with severe acute pancreatitis (SAP). METHODS: SAP rats were randomly assigned to the model group and treatment group while the normal rats were assigned to the sham operation group. The mortality,ascite volumes,ascites/body weight ratio and pancreas pathological changes of all rats were observed at 3,6 and 12 h after operation. Their contents of amylase and endotoxin in plasma and contents of tumor necrosis factor (TNF-α),phospholipase A2 (PLA2) and IL-6 in serum were also determined. The microarray sections of their pancreatic tissues were prepared,terminal transferase dUTP nick end labeling (TUNEL) staining was performed and apoptotic indexes were calculated. RESULTS: There was no marked difference between treatment group and model group in survival. The contents of amylase and endotoxin in plasma and contents of TNF-α,PLA2 and IL-6 in serum,ascite volumes,ascites/body weight ratio and pancreas pathological scores were all lower in treatment group than in model group to different extents at different time points P < 0.05,58.3 (26.4) ng/L vs 77.535 (42.157) ng/L in TNF-α content,8.00 (2.00) points vs 9.00 (2.00) points in pathological score of pancreas respectively; P < 0.01,0.042 (0.018) EU/mL vs 0.056 (0.0195) EU/mL in endotoxin content,7791 (1863) U/L vs 9195 (1298) U/L in plasma amylase content,1.53 (0.79) vs 2.38 (1.10) in ascites/body weight ratio,8.00 (1.00) points vs 11.00 (1.50) points in pathological score of pancreas; P < 0.001,3.36 (1.56) ng/L vs 5.65 (1.08) ng/L in IL-6 content,4.50 (2.00) vs 7.20 (2.00),4.20 (1.60) vs 6.40 (2.30),3.40 (2.70) vs 7.90 (1.70) in ascite volumes,respectively. The apoptotic indexes of pancreas head and pancreas tail were all higher in treatment group than in model group at 6 h P < 0.01,0.00 (2.00)% vs 0.00 (0.00)%,0.20 (1.80) vs 0.00 (0.00) in apoptosis indexes,respectively. CONCLUSION: The mechanism of dexamethasone treatment in acute pancreatitis is related to its inhibition of inflammatory mediator generation and induction of pancreatic acinar cell apoptosis. | Xi-Ping Zhang Li Chen Qi-Fang Hu Hua Tian Ru-Jun Xu Zhi-Wei Wang Ke-Yi Wang Qi-Hui Cheng Wei Yan Yun Li Qing-Yu Li Qing He Fei Wang | 2007 | World Journal of Gastroenterology2007，13，41： | 29 |
| 13 | Effectiveness and safety of noninvasive positive-pressure ventilation for severe hypercapnic encephalopathy due to acute exacerbation of chronic obstructive pulmonary disease:a prospective case-control study显示文摘背景尽管严重 encephalopathy 作为可能的禁止徵候被建议了到 noninvasive 的使用积极压力的通风( NPPV ),增加的临床的报告证明它在有损害知觉甚至对尖锐呼吸失败第二等的昏迷的病人是有效的,特别 hypercapnic 尖锐呼吸失败( HARF ) .To 进一步为严重 hypercapnic encephalopathy 评估 NPPV 的有效性和安全。 | ZHU Guang-fa ZHANG Wei ZONG Hua XU Qiu-fen LIANG Ying | 2007 | Chinese Medical Journal2007，，24： | 30 |
| 14 | Mycoplasma pneumoniae pneumonia in hospitalized children diagnosed at acute stage by paired sera显示文摘背景霉浆菌 pneumoniae (M。pneumoniae ) 是呼吸的道感染的一个经常的原因。然而, M 的临床的表明有缺乏的知识。pneumoniae 感染。我们描述了临床并且 M 的实验室调查结果。在都被抗体 titer.Methods M 的四折的增加诊断的就医的孩子的 pneumoniae 肺病。pneumoniae 抗体习惯性地在在一个一个年时期期间与急性呼吸感染招收的孩子被检测。病历是从孩子的 re-collected 谁的 M。在由一个单身的人的床边的 pneumoniae 抗体 titer increasedfourfold,和他们的冻结的配对的浆液样品为其 sera 为 M 被检测的 635 个孩子的 M.pneumoniae 抗体 titer.Results 再被测量。pneumoniae 抗体,配对的 sera 从 82 和 29.3% 被获得(24/82 ) 在抗体 titer 显示出四折的增加。有 24 个盒子,九个男孩和 15 个女孩,从二变老到 14 年,浆液样品在在的白天 9 上被花其秒在症状发作以后最早;最短的间隔是三天。与高发烧(38.5 ) 介绍的所有孩子并且咳嗽。21 没有鼻音阻塞或一个流鼻涕的鼻子,并且五有都与高发烧被联系的温和头疼。如果山峰温度是 39.5,疾病是比较地严重的。所有作为通过胸 X 光有肺病被诊断。四有双边或 multilobar 参与和他们的山峰温度都是 39.5。任何一个都没在呼吸孩子们有困难并且都没显示出第二件浆液样品能在在的白天 9 上被拿的 wheezing.Conclusions 的迹象在意味着的症状发作以后最早那配对的 sera 能被用于 M 的临床的诊断。在在尖锐阶段的孩子的 pneumoniae 肺病。M。pneumoniae 是更低的呼吸的道病原体。Extrapulmonary 复杂并发症在我们的学习稀罕、次要。高山峰温度(39.5 ) 与 M 的严厉被相关。在孩子的 pneumoniae 肺病。 | LIU Chun-ling WANG Gui-qiang ZHANG Bo XU Hua HU Liang-ping HE Xiao-feng WANG Jun-hua ZHANG Jun-hong LIU Xiao-yu WEI Ming LIU Zhen-ye | 2010 | Chinese Medical Journal2010，，23： | 30 |
| 15 | 5-Hydroxymethylcytosine signatures in circulating cell-free DNA as diagnostic biomarkers for human cancers显示文摘象 5-methylcytosine (5mC ) 和 5-hydroxymethylcytosine (5hmC ) 那样的 DNA 修正是知道在哺乳动物影响全球基因表示的 epigenetic 标记。在人的染色体,与基因表示的靠近的关联和高化学的稳定性给他们的流行,这些 DNA epigenetic 标记能为癌症用作理想的 biomarkers 诊断。利用一种高度敏感、选择的化学标记技术,我们这里报导在传播没有房间的 DNA ( cfDNA )并且在从最近与 colorectal 诊断的 260 个病人的一个队收集的配对的肿瘤和邻近的纸巾的 genomic DNA ( gDNA )的 5hmC 的染色体宽的介绍,胃,胰腺,从 90 个健康个人的肝或甲状腺癌症和正常纸巾。5hmC 主要在与开的染色质和容许的 histone 修正重合的 transcriptionally 活跃的区域被散布。柔韧的联系癌症的 5hmC 签名在 cfDNA 被识别为特定的癌症类型是特征的。传播 cfDNA 的 5hmC 底 biomarkers colorectal 和胃的癌症是高度预兆的并且比常规 biomarkers 优异并且比得上从织物活体检视的 5hmC biomarkers。因此,这新策略能从血样品的分析为癌症的诊断和预后导致有效、最低限度地侵略的方法的发展。 | Wenshuai Li Xu Zhang Xingyu Lu Lei You Yanqun Song Zhongguang Luo Jun Zhang Ji Nie Wanwei Zheng Diannan Xu Yaping Wang Yuanqiang Dong Shulin Yu Jun Hong Jianping Shi Hankun Hao Fen Luo Luchun Hua Peng Wang Xiaoping Qian Fang Yuan Lianhuan Wei Ming Cui Taiping Zhang Quan Liao Menghua Dai Ziwen Liu Ge Chen Katherine Meckel Sarbani Adhikari Guifang Jia Marc B Bissonnette Xinxiang Zhang Yupei Zhao Wei Zhang Chuan He Jie Liu | 2017 | Cell Research2017，27，10： | 30 |
| 16 | Quantitative analysis of hepatic hypoxia-inducible factor-1α and its abnormal gene expression during the formation of hepatocellular carcinoma显示文摘BACKGROUND:Hepatic hypoxia-inducible factor-1(HIF-1) is activated in the progression of hepatocellular carcinoma (HCC).This study aimed to investigate the dynamic alterations of HIF-1αand its gene expression so as to explore the relationship between HIF-1αexpression and hepatocarcinogenesis at the early stage of HCC. METHODS:A hepatoma model was made with 2-fluorenyl- acetamide(2-FAA)in male Sprague-Dawley rats.Morphological changes of rat hepatocytes were assessed pathologically (HE staining).The dynamic expression of hepatic and circulating HIF-1αwas quantitatively analyzed by ELISA. The gene fragments of hepatic HIF-1αmRNA were amplified by RT-PCR and confirmed by sequencing.The cellular distribution of hepatic HIF-1αexpression was confirmed by immunohistochemistry. RESULTS:Histological examination confirmed granulelike degeneration to atypical hyperplasia and HCC development in rat hepatocytes and progressive increases in the levels of hepatic and circulating HIF-1αand its gene expression during the course.The levels of HIF-1α expression in the liver and blood of rats with hepatoma were significantly higher than those in normal ratsand those with degeneration.Immunohistochemical analysis confirmed the positive expression and hepatocyte distribution of HIF-1αin the development of rat hepatoma. A positive relationship was found between HIF-1α expression in the liver and blood(P<0.01). CONCLUSIONS:The above observations support the hypothesis that the overexpression of HIF-1αand its gene are closely associated with the malignant transformation of hepatocytes and play an important role at the stage of hepatocarcinogenesis. | Deng-Fu Yao,Hua Jiang,Min Yao,Yue-Ming Li,Wen-Jing Gu,Yu-Cheng Shen, Li-Wei Qiu,Wei Wu,Xin-Hua Wu and Wen-Li Sai Research Center of Clinical Molecular Biology,Department of Laboratory Science,and Department of Oncology,Affiliated Hospital of Nantong University,Nantong 226001, China Third Department of Medicine,Jiaonan People’s Hospital,Jiaonan 266400,China | 2009 | Hepatobiliary & Pancreatic Diseases International2009，8，4： | 28 |
| 17 | Asymptotically Optimal Scenario Analysis and Wait-and-See Model for Optimal Power Flow With Wind Power显示文摘精确模拟风功率分布的场景,有效求解反映风电随机性的最优潮流模型,对大规模风电并网的经济调度、质量控制及安全运行均具有重要意义。引入风电随机变量,建立最优潮流的"wait-and-see"(WS-OPF)模型;采用优于其他指标的瓦瑟斯坦(Wasserstein)距离为衡量指标,形成与风功率概率分布最优近似的场景模型,生成渐近最优场景,获得潮流分布及机组最优出力等指导调度安全经济运行的重要信息。最后,以IEEE 30和IEEE 300系统为例,对比各种距离指标的模拟精度,验证Wasserstein距离的优良性;同时,与不考虑风电随机性的模型相比,WS-OPF模型能更好地模拟风功率随机特性,提供与实际发生的风功率相近的调度方案,从而合理地规避风电接入电网的风险,提高风电的消纳能力。 | LI Jinghua WEI Hua MO Dong | 2012 | 中国电机工程学报2012，32，22： | 27 |
| 18 | Landscape Pattern Evolution Processes of Alpine Wetlands and Their Driving Factors in the Zoige Plateau of China显示文摘Zoige Plateau wetlands are located in the northeastern corner of the Qinghai-Tibet Plateau.The landscape pattern evolution processes in the Zoige Plateau and their driving factors were identified by analyzing the dynamic changes in landscape modification and conversion and their dynamic rates of alpine wetlands over the past four decades.The results showed that the landscape conversion between wetlands and non-wetlands mainly occurred during the period from 1966 to 1986.The marsh wetland area converted from lake and river wetlands was larger because of swamping compared to other wetland landscapes.Meanwhile,the larger area of marsh wetlands was also converted to lake wetlands more than other types of wetlands.The modification processes mainly occurred among natural wetland landscapes in the first three periods.Obvious conversions were observed between wetland and nonwetland landscapes(i.e.,forestland,grassland,and other landscapes) in the Zoige Plateau.These natural wetland landscapes such as river,lake and marsh wetlands showed a net loss over the past four decades,whereas artificial wetland landscapes(i.e.,paddy field and reservoir and pond wetlands) showed a net decrease.The annual dynamic rate of the whole wetland landscape was 0.72%,in which the annual dynamic rate of river wetlands was the highest,followed by lake wetlands,while marsh wetlands had the lowest dynamic rate.The integrated landscape dynamic rate showed a decreasing trend in the first three periods.The changes in wetland landscape patterns were comprehensively controlled by natural factors and human activities,especially human activities play an important role in changing wetland landscape patterns. | BAI Jun-hong LU Qiong-qiong WANG Jun-jing ZHAO Qing-qing OUYANG Hua DENG Wei LI Ai-nong | 2013 | Journal of Mountain Science2013，10，1： | 28 |
| 19 | Serum microRNA 125b as a diagnostic or prognostic biomarker for advanced NSCLC patients receiving cisplatin-based chemotherapy显示文摘 | En-hai CUI Hong-jiao LI Feng HUA Bin WANG Wei MAO Xue-ren FENG Jian-you LI Xiang WANG | 2013 | Acta Pharmacologica Sinica2013，34，2： | 28 |
| 20 | Rapeseed research and production in China显示文摘Rapeseed(Brassica napus L.) is the largest oilseed crop in China and accounts for about 20% of world production.For the last 10 years,the production,planting area,and yield of rapeseed have been stable,with improvement of seed quality and especially seed oil content.China is among the leading countries in rapeseed genomic research internationally,having jointly with other countries accomplished the whole genome sequencing of rapeseed and its two parental species,Brassica oleracea and Brassica rapa.Progress on functional genomics including the identification of QTL governing important agronomic traits such as yield,seed oil content,fertility regulation,disease and insect resistance,abiotic stress,nutrition use efficiency,and pod shattering resistance has been achieved.As a consequence,molecular markers have been developed and used in breeding programs.During 2005–2014,215 rapeseed varieties were registered nationally,including 210 winter-and 5 spring-type varieties.Mechanization across the whole process of rapeseed production was investigated and operating instructions for all relevant techniques were published.Modern techniques for rapeseed field management such as high-density planting,controlled-release fertilizer,and biocontrol of disease and pests combined with precision tools such as drones have been developed and are being adopted in China.With the application of advanced breeding and production technologies,in the near future,the oil yield and quality of rapeseed varieties will be greatly increased,and more varieties with desirable traits,especially early maturation,high yield,high resistance to biotic and abiotic stress,and suitability for mechanized harvesting will be developed.Application of modern technologies on the mechanized management of rapeseed will greatly increase grower profit. | Qiong Hu Wei Hua Yan Yin Xuekun Zhang Lijiang Liu Jiaqin Shi Yongguo Zhao Lu Qin Chang Chen Hanzhong Wang | 2017 | The Crop Journal2017，5，2： | 25 |
您所在的机构暂未开通此部分的功能，请联系管理员