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| 1 | Mitotic motor CENP-E cooperates with PRC1 in temporal control of central spindle assembly显示文摘Error-free cell division depends on the accurate assembly of the spindle midzone from dynamic spindle microtubules to ensure chromatid segregation during metaphase-anaphase transition.However,the mechanism underlying the key transition from the mitotic spindle to central spindle before anaphase onset remains elusive.Given the prevalence of chromosome instability phenotype in gastric tumorigenesis,we developed a strategy to model context-dependent cell division using a combination of light sheet microscope and 3D gastric organoids.Light sheet microscopic image analyses of 3D organoids showed that CENP-E inhibited cells undergoing aberrant metaphase-anaphase transition and exhibiting chromosome segregation errors during mitosis.Highresolution real-time imaging analyses of 2D cell culture revealed that CENP-E inhibited cells undergoing central spindle splitting and chromosome instability phenotype.Using biotinylated syntelin as an affinity matrix,we found that CENP-E forms a complex with PRC1 in mitotic cells.Chemical inhibition of CENP-E in metaphase by syntelin prevented accurate central spindle assembly by perturbing temporal assembly of PRC1 to the midzone.Thus,CENP-E-mediated PRC1 assembly to the central spindle constitutes a temporal switch to organize dynamic kinetochore microtubules into stable midzone arrays.These findings reveal a previously uncharacterized role of CENP-E in temporal control of central spindle assembly.Since CENP-E is absent from yeast,we reasoned that metazoans evolved an elaborate central spindle organization machinery to ensure accurate sister chromatid segregation during anaphase and cytokinesis. | Xu Liu Leilei Xu Junying Li Phil Y.Yao Wanjuan Wang Hazrat Ismail Haowei Wang Bryce Liao Zhihong Yang Tarsha Ward Ke Ruan Jianchun Zhang Quan Wu Ping He Xia Ding Dongmei Wang Chuanhai Fu Zhen Dou Feng Yan Wenwen Wang Xing Liu Xuebiao Yao | 2020 | Journal of Molecular Cell Biology2020，12，8： | 3 |
| 2 | Combination of antidepressantmedications from treatment initiation for major depressive disorder:a double-blind randomized study显示文摘 | Blier P Ward HE Tremblay P | 2010 | Am J Psychiatry2010，167，3： | 1 |
| 3 | The prevalence of PAX-2 mutations in patients with isolated colobomas or colobomas associated with urogenital abnomalies显示文摘 | Cunliffe HE McNoe LA Ward TA | 1998 | J Med Genet1998，35，： | 1 |
| 4 | The pulmonary response to sublethal thoracic irradiation in the rat显示文摘 | Ward HE Kemsley L Davies L | 1993 | Radiation Research1993，136，1： | 1 |
| 5 | Analysis of improvement in individual rheumatoid arthritis patients treated with disease-modifying antirheumatic drugs, based on findings in patients treated with placebo 显示文摘 | Paulus HE Egger MJ Ward JR | 1990 | Arthritis Rheum1990，33，4： | 1 |
| 6 | 显示文摘 | Ward E Wolfsen HE Nq C | 2002 | South Med J2002，95，2： | 1 |
| 7 | Analysis of premalignantpancreatic cancer mass spectrometry data for biomarker se-lection using a group search optimizer显示文摘 | He S Cooper H J Ward D G | 2011 | Transaction of theInstitute of Measurement and Control2011，34，6： | 1 |
| 8 | Analysis of improvement in Individual rheumatoid arthritis patients treated with disease-modi fying drugs, based on the finding in patients treated with placebo 显示文摘 | Paulus HE Egger ML Ward JR | 1990 | Arthtitis Rheum1990，33，： | 1 |
| 9 | Summary of the clinical pharmacokinetics of the prodrug oseltamivir and its active metabolite (Ro 64-0802)显示文摘 | Massarella J Ward P | 1999 | Clin Pharmacokinet1999，37，： | 1 |
| 10 | Update on deep brain stimulation for neuropsychiatric disorders显示文摘 | Ward HE Hwynn N Okun MS | 2010 | Neurobiol Dis2010，38，： | 1 |
| 11 | A pilot study of vagus nerve stimulation(VNS)for treatmentresistant anxiety disorders显示文摘 | George MS Ward HE Jn Ninan PT | | 0，，02： | 1 |
| 12 | A double-blind,placebo-controlled trial of olanzapine addition in fluoxeitine-refractory obsessive-compulsive disorder显示文摘 | Shapira NA Ward HE Mandoki M | 2004 | Biol Psychiatry2004，55，5： | 1 |
| 13 | Clinical pharmacokinetics of the prodrug oseltamivir and its active metabolite Ro 64-0802显示文摘 | Massarella J Ward P | 1999 | Clin Pharmacokinet1999，37，： | 1 |
| 14 | Combination of antidepressant medications from treatment initiation for major depressive disorder: a double-blind randomized study显示文摘 | Blier P Ward HE Tremblay P | 2010 | American Journal of Psychiatry2010，167，3： | 1 |
| 15 | A double-blind, placebo-controlled trial of olanzapine addition in fluoxeifine- refractory obsessive-compulsive disorder显示文摘 | Shapira NA Ward HE M andoki M | 2004 | Biol Psychiatry2004，55，5： | 1 |
| 16 | Recovery of infectious SV5 from cloned DNA and expression of a foreign gene显示文摘 | He B Paterson R G Ward C D | 1997 | Virol1997，237，： | 1 |
| 17 | Combination of antidepressant med- ications from treatment initiation for major depressive disorder: a double- blind randomized study 显示文摘 | Blier P Ward HE Tremblay P | 2010 | Am J Psychiatry2010，167，3： | 1 |
| 18 | Combination of antidepressant medications from treatment initiation for major depressive disorder: a double-blind randomized study 显示文摘 | Blier P Ward HE Tremblay P | 2010 | Am J Psychiatry2010，167，3： | 1 |
| 19 | A double-blind,placebocontrolled trial of olanzapine addition in fluoxeifine-refractory obsessive-compulaive disorder显示文摘 | Shapira NA Ward HE Mandoki M | 2004 | Biol Psychiatry2004，55，5： | 1 |
| 20 | Ligand activation of peroxisome proliferator-activated receptor beta inhibits colon carcinogenesis显示文摘 | Marin HE Peraza MA Billin AN Willson TM Ward JM Kennett MJ | 2006 | Cancer Res2006，66，8： | 1 |
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