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| 1 | Identification of HBsAg-specific antibodies from a mammalian cell displayed full-length human antibody library of healthy immunized donor显示文摘肝炎 B 免疫球蛋白(HBIG ) 在肝炎 B 的管理是重要的病毒(HBV ) 感染。试图作为 HBIG 的一种选择开发 recombinant monoclonal 抗体,我们报导 HBV 表面抗原(HBsAg ) 的成功的鉴定从在哺乳动物的房间上显示的一个全身的人的抗体图书馆的特定的抗体出现。把一个生来地使免疫的施主的外部血 mononuclear 房间的全部的 RNA 用作模板,抗体全部剧目被放大。联合四方法的结扎和 Flp 调停 recombinase 的集成(Flp 在里面) 系统,我们构造了每个房间在显示了一个种仅仅抗体分子的一个哺乳动物的基于房间的、充分人的、全身的抗体显示图书馆。由屏蔽使用激活荧光的房间排序的房间图书馆(FACS ) ,八个房间克隆显示那房间表面上的 HBsAg 特定的抗体被识别。抗体基因的 DNA 顺序分析揭示了三唯一的抗体。FACS 数据显示了那荧光灯表示(FSE ) 的力量,荧光灯绑定(FSB ) 的力量和相对有约束力的能力(RBA ) 都在他们之中是不同的。这些结果由使用我们的抗体表明了那哺乳动物的显示和屏蔽站台,我们能成功地从一个使免疫的全身的抗体图书馆识别抗原特定的抗体。因此,这个平台为治疗学的抗体的开发是很有用的。 | Chang-Zheng Li Zhong-Kun Liang Zhen-Rui Chen Hai-Bo Lou Ye Zhou Zhe-Huan Zhang Fei Yu Shuwen Liu Yuanping Zhou Shuguang Wu Wenling Zheng Wanlong Tan Shibo Jiang Chen Zhou | 2012 | Cellular & Molecular Immunology2012，9，2： | 7 |
| 2 | Four-way ligation for construction of a mammalian cell-based full-length antibody display library显示文摘唯一的四方法的结扎策略为一个全身的抗体图书馆的快速的建设被开发。哺乳动物的表示向量被构造盒子和序列由唯一的限制酶 BsmBI, BstXI,和 SfiI 认出了的那包含的双哺乳动物的表情。重链的基因的全身的轻链、可变的领域被四方法的结扎在一步插入到向量,并且全身的二原子价的抗体在哺乳动物的房间表面上被显示。用这策略,仅仅 2 个星期被要求成功地构造高质量的、全身的人的抗体图书馆。 | Ivan Zhou Zhe-Huan Zhang Chang-Zheng Li Zhen-Rui Chen Wei He Ye Zhou Shuwen Liu Shuguang Wu Yuanping Zhou Wanlong Tan Shibo Jiang Chen Zhou | 2011 | Acta Biochimica et Biophysica Sinica2011，43，3： | 6 |
| 3 | Improving the Local Sheep in Gansu via Crossing with Introduced Sheep Breeds Dorset and Borderdale显示文摘In order to improve the meat performance of local sheep in Gansu Province,Dorset and Borderdale were introduced to crossbreed with local sheep which were Tan sheep,Small-tail Han sheep and Mongolia sheep. The offspring under different crossbreeding combinations were sampled randomly at the different growing stage to measure their growth traits so as to select optimize the crossbreeding mode. The results indicated that,for the same crossbreeding mode,the growth rate of progeny was in order F3> F2> F1; for the F3 progeny,the combinations Dorset- Borderdale- Small tail Han sheep and Dorset- Borderdale- Mongolia sheep gave a higher growth rate,with a body weight of 1. 57%,3. 17%,8. 23%,1. 15% higher in male and female individuals than the counterparts of Dorset and Tan sheep and Small tail Han sheep; for the F2 progeny,the combinations Dorset- Borderdale- Small tail Han sheep and Dorset- Borderdale- Mongolia sheep also gave a higher growth rate,with a body weight of 2. 15%,4. 53%,9. 21% and 2. 75% higher in male and female individuals than the counterparts of Dorset and Tan sheep and Small tail Han sheep; for the F1 progeny,the combination Borderdale and Small tail Han sheep assumed a higher growth rate,with a body weight of 3. 23%,6. 07%,7. 42% and 8. 66% higher in male and female individuals than the counterparts of Borderdale- Mongolia sheep and Tan sheep- Small tail Han sheep,respectively. Therefore,in the Small-tail Han sheep and Mongolia sheep producing regions,the F2 or F3progeny bred by using Dorset or Borderdale sheep as male parent to cross with local breeds,or the hybrid lambs of Small-tail Han sheep and Borderdale sheep as highly qualified commodity,would produce significant economic benefit. Moreover,the novel breeds obtained by crossing were the valuable genetic resource for breeding meat sheep. | Sun Xiaoping Liu Jianbin Zhang Wanlong Lang Xia Yang Bohui Guo Jian Feng Ruilin | 2014 | Animal Husbandry and Feed Science2014，6，5： | 3 |
| 4 | Hybrid Dirac semimetal-based photodetector with efficient low-energy photon harvesting显示文摘Despite the considerable effort,fast and highly sensitive photodetection is not widely available at the low-photon-energy range(~meV)of the electromagnetic spectrum,owing to the challenging light funneling into small active areas with efficient conversion into an electrical signal.Here,we provide an alternative strategy by efficiently integrating and manipulating at the nanoscale the optoelectronic properties of topological Dirac semimetal PtSe_(2)and its van der Waals heterostructures.Explicitly,we realize strong plasmonic antenna coupling to semimetal states near the skin-depth regime(λ/10^(4)),featuring colossal photoresponse by in-plane symmetry breaking.The observed spontaneous and polarization-sensitive photocurrent are correlated to strong coupling with the nonequilibrium states in PtSe_(2)Dirac semimetal,yielding efficient light absorption in the photon range below 1.24 meV with responsivity exceeding∼0.2 A/W and noise-equivalent power(NEP)less than~38 pW/Hz^(0.5),as well as superb ambient stability.Present results pave the way to efficient engineering of a topological semimetal for high-speed and low-energy photon harvesting in areas such as biomedical imaging,remote sensing or security applications. | Lin Wang Li Han Wanlong Guo Libo Zhang Chenyu Yao Zhiqingzi Chen Yulu Chen Cheng Guo Kaixuan Zhang Chia-Nung Kuo Chin Shan Lue Antonio Politano Huaizhong Xing Mengjie Jiang Xianbin Yu Xiaoshuang Chen Wei Lu | 2022 | Light(Science & Applications)2022，11，3： | 2 |
| 5 | Study on Flow Characteristics of a Turbulent Boundary Layer and Vortex Structure of High Pressure Guide Vanes in SCO_2 Turbines显示文摘In order to further understand the aerothermodynamic performance and flow loss mechanism of SCO_2 turbines, RANS equations and an SST Turbulence Model were chosen for a numerical study on the secondary flow and vortex structure of cascades using the commercial software CFX. The dimensionless vorticity analysis method was used to study the flow characteristics of the logarithmic layer and viscous sublayer in high pressure guide vane cascades. The new vortex structure and formation mechanism of the vortices were given and analyzed. Simulation results indicated that during the motion of the boundary layer in the cascades, the logarithmic layer and viscous sublayer obtain the different rotational direction vorticity, respectively. The endwall logarithmic layer and pressure side leg of the horseshoe votex gradually develop into the passage vortex, with the endwall viscous sublayer gradually developing into the corner viscous sublayer vortex II and the endwall viscous sublayer vortex I. The endwall viscous sublayer that rolled by the passage vortex is encountered with the upstream-side and radial boundary layer of the vane at the suction separation line, forming the suction separation line vortex beside the passage vortex. A pair of radial transition vortices are formed between the wake and the main stream. | HAN Wanlong WANG Yueming FENG Zhenping LI Hongzhi YAO Mingyu ZHANG Yifan | 2019 | Journal of Thermal Science2019，28，3： | 1 |
| 6 | A Novel Therapeutic Vaccine of Mouse GM-CSF Surface Modified MB49 Cells Against Metastatic Bladder Cancer显示文摘 | Xinji Zhang Xiaojun Shi Jinlong Li Zhiming Hu Dong Zhou Jimin Gao Wanlong Tan | 2012 | The Journal of Urology2012，，3： | 1 |
| 7 | Improvement of Low OCV in MH/Ni Battery显示文摘In MH/Ni high power battery production, a large percent of batteries were found with an open circuit voltage lower than 1.18 V after formation, open circuit voltage of another large percent of batteries could be even as low as1.00 ~ 1.10 V after one month sitting.In this situation, we analysed the causes of the problem, and provided some efficient solution based on many experiments.It was proved that these solutions are working very well to prevent the low open circuit voltage and being used in real production. | Zhang Zhong Liang Wanlong Zhang Weihua Xing Zhiqiang Ma Yijun | 2004 | Journal of Rare Earths2004，22，z1： | 0 |
| 8 | Photo-induced flexible semiconductor CdSe/CdS quantum rods alignment显示文摘The anisotropic absorption and emission from semiconductor CdSe/CdS quantum rods(QRs)provide extra benefits among other photoluminescence nanocrystals.Using photo-induced alignment technique,the QRs can be oriented in liquid crystal polymer matrix at a large scale.In this article,a 2D Dammann grating pattern,within“SKL”characters domains aligned QRs in composite film,was fabricated by multi-step photo exposure using several photo masks,and a continuous geometric lens profile pattern aligned QRs was realized by the single step polarization converting holographic irradiation method.Both polarized optical microscope and fluorescence microscope are employed to determine the liquid crystal director profiles and QRs anisotropic excitation properties.We have been able to orient the QRs in fine binary and continuous patterns that confirms the strong quantum rod aligning ability of the proposed method.Thus,the proposed approach paves a way for photoinduced flexible QRs alignments to provide a highly specific and difficult-to-replicate security application at a large scale. | Wanlong Zhang Julian Schneider Maksym F.Prodanov Valerii V.Vashchenko Andrey L.Rogach Xiaocong Yuan Abhishek K.Srivastava | 2023 | Journal of Semiconductors2023，44，9： | 0 |
| 9 | Research on Eliminate Micro-Shorted MH/Ni Batteries显示文摘There are two methods for selecting micro-shorted MH/Ni batteries out from all formationed.cells.One is to judge by the decrease of open circuit voltage which takes longer standing time to eliminate efficiently and does not work very well when the coverage of open circuit voltage is big.Another is to judge by discharge capacity of charged cells, this can not only meet the requirement of MH/Ni battery stored in charged state, but also has the advantages of being easier to read out, good accuracy and taking shorting standing time etc, is a proper way to be used on the production line. | Liang Wanlong Zhang Zhong Xu Shaoping Ma Yijun | 2004 | Journal of Rare Earths2004，22，z1： | 0 |
| 10 | Population genomics provides insights into the evolution and adaptation of tree shrews(Tupaia belangeri)in China显示文摘Physiological adaptation of tree shrews(Tupaia belangeri)to changing environmental temperature has been re-ported in detail.However,the T.belangeri origin(mainland or island),population history,and adaptation to histor-ical climate change remain largely unknown or controversial.Here,for thefirst time,we sequenced the simplified genome of 134 T.belangeri individuals from 12 populations in China and further resequenced one individual from each population.Using population genomic approaches,wefirst observed considerable genetic variation in T.be-langeri.Moreover,T.belangeri populations formed obvious genetic structure and reflected different demographic histories;they generally exhibited high genetic diversity,although the isolated populations had relatively low ge-netic diversity.The results presented in this study indicate that T.b.modesta and T.b.tonquinia were separated recently and with a similar population dynamics.Second,physical barriers rather than distance were the driving factors of divergence,and environmental heterogeneity may play an important role in genetic differentiation in T.belangeri.Moreover,our analyses highlight the role of historical global climates in the T.belangeri population dynamics and indicate that the decrease of the T.belangeri population size may be due to the low temperature.Finally,we identified the olfaction-associated adaptive genes between different altitude populations and found that olfactory-related genes of high-altitude populations were selectively eliminated.Our study provides demographic history knowledge of T.belangeri;their adaption history offers new insights into their evolution and adaptation,and provides valuable baseline information for conservation measures. | Yue REN Ting JIA Hao ZHANG Wanlong ZHU Zhengkun WANG | 2023 | Integrative Zoology2023，18，1： | 0 |
| 11 | Interactive information disclosure and non-penalty regulatory review risk显示文摘Using monthly data from the Shenzhen Stock Exchange's‘Hudongyi’platform and comment letters from December 2014 to December 2018,this study investigates the influence of interactive information disclosure on non-penalty regulatory review risk.The findings reveal that the richness and activeness of interactive information disclosure are positively associated with regulatory review risk.Moreover,the non-penalty regulatory review is effective as it significantly reduces the probability of receiving a comment letter in the subsequent three periods.The timeliness of interactive information disclosure is negatively associated with regulatory review risks.Additionally,we find that newspaper media coverage partially mediates the relationship between interactive information disclosure and regulatory review risk.For companies with low levels of internal governance,in low-competitive industries,and state-owned companies,the positive relationship between the number of investor questions and regulatory review risk is strengthened.These findings enrich the literature on the determinants of regulatory review risk and the economic consequences of interactive information disclosure in emerging markets. | Meng Wang Yongjie Zhang Yizhe Dong Gaofeng Zou Wanlong Zhao | 2023 | Journal of Management Science and Engineering2023，8，1： | 0 |
| 12 | Characterization of interfaces: Lessons from the past for the future of perovskite solar cells显示文摘A photovoltaic technology historically goes through two major steps to evolve into a mature technology. The first step involves advances in materials and is usually accompanied by the rapid improvement of power conversion efficiency. The second step focuses on interfaces and is usually accompanied by significant stability improvement. As an emerging generation of photovoltaic technology, perovskite solar cells are transitioning to the second step of their development when a significant focus shifts toward interface studies and engineering. While various interface engineering strategies have been developed, interfacial characterization is crucial to show the effectiveness of interfacial modification. Here, we review the characterization techniques that have been utilized in studying interface properties in perovskite solar cells. We first summarize the main roles of interfaces in perovskite solar cells, and then we discuss some typical characterization methodologies for morphological, optical,and electrical studies of interfaces. Successful experiences and existing problems are analyzed when discussing some commonly used methods. We then analyze the challenges and provide an outlook for further development of interfacial characterizations. This review aims to evoke strengthened research devotion on novel and persuasive interfacial engineering. | Wanlong Wang Dongyang Zhang Rong Liu Deepak Thrithamarassery Gangadharan Furui Tan Makhsud I.Saidaminov | 2022 | Journal of Semiconductors2022，43，5： | 0 |
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