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| 1 | A novel strategy for rapid construction of libraries of full-length antibodies highly expressed on mammalian cell surfaces显示文摘一个万用的哺乳动物的显示器系统的开发为有高亲密关系的功能的人的抗体的选择是必要的。这里,我们为能高效地在哺乳动物的房间表面上被表示的全身的抗体图书馆的快速的建设描述了新奇策略。通用向量 pDGB-HC-TM 被插入唯一的序列为兴趣的基因的突然发生在里面和突然发生外面由限制 endonucleases BsmBI, SfiI,和 BstXI 认出了的多重克隆的地点构造。 Cytomegalovirus 倡导者,为在许多哺乳动物的房间的蛋白质的高表示的一个通常使用的倡导者,被用来从导出血小板的生长驾驶插入的抗体基因和一个 transmembrane 领域的表示因素受体在框架被熔化到重链的C终点一致区域到抗体在哺乳动物的房间表面上表示了的锚。用这策略,我们构造了一个全身的人的抗体显示图书馆。DNA 顺序分析和表示分析显示图书馆构造了有 6.58 e 的组合可表现的、可检测的差异 ???汩 l 吗?? | Ye Zhou Zhen-Rui Chen Chang-Zheng Li Wei He Shuwen Liu Shibo Jiang Wen-Li Ma Wanlong Tan Chen Zhou | 2010 | Acta Biochimica et Biophysica Sinica2010，42，8： | 11 |
| 2 | Identification of HBsAg-specific antibodies from a mammalian cell displayed full-length human antibody library of healthy immunized donor显示文摘肝炎 B 免疫球蛋白(HBIG ) 在肝炎 B 的管理是重要的病毒(HBV ) 感染。试图作为 HBIG 的一种选择开发 recombinant monoclonal 抗体,我们报导 HBV 表面抗原(HBsAg ) 的成功的鉴定从在哺乳动物的房间上显示的一个全身的人的抗体图书馆的特定的抗体出现。把一个生来地使免疫的施主的外部血 mononuclear 房间的全部的 RNA 用作模板,抗体全部剧目被放大。联合四方法的结扎和 Flp 调停 recombinase 的集成(Flp 在里面) 系统,我们构造了每个房间在显示了一个种仅仅抗体分子的一个哺乳动物的基于房间的、充分人的、全身的抗体显示图书馆。由屏蔽使用激活荧光的房间排序的房间图书馆(FACS ) ,八个房间克隆显示那房间表面上的 HBsAg 特定的抗体被识别。抗体基因的 DNA 顺序分析揭示了三唯一的抗体。FACS 数据显示了那荧光灯表示(FSE ) 的力量,荧光灯绑定(FSB ) 的力量和相对有约束力的能力(RBA ) 都在他们之中是不同的。这些结果由使用我们的抗体表明了那哺乳动物的显示和屏蔽站台,我们能成功地从一个使免疫的全身的抗体图书馆识别抗原特定的抗体。因此,这个平台为治疗学的抗体的开发是很有用的。 | Chang-Zheng Li Zhong-Kun Liang Zhen-Rui Chen Hai-Bo Lou Ye Zhou Zhe-Huan Zhang Fei Yu Shuwen Liu Yuanping Zhou Shuguang Wu Wenling Zheng Wanlong Tan Shibo Jiang Chen Zhou | 2012 | Cellular & Molecular Immunology2012，9，2： | 7 |
| 3 | Four-way ligation for construction of a mammalian cell-based full-length antibody display library显示文摘唯一的四方法的结扎策略为一个全身的抗体图书馆的快速的建设被开发。哺乳动物的表示向量被构造盒子和序列由唯一的限制酶 BsmBI, BstXI,和 SfiI 认出了的那包含的双哺乳动物的表情。重链的基因的全身的轻链、可变的领域被四方法的结扎在一步插入到向量,并且全身的二原子价的抗体在哺乳动物的房间表面上被显示。用这策略,仅仅 2 个星期被要求成功地构造高质量的、全身的人的抗体图书馆。 | Ivan Zhou Zhe-Huan Zhang Chang-Zheng Li Zhen-Rui Chen Wei He Ye Zhou Shuwen Liu Shuguang Wu Yuanping Zhou Wanlong Tan Shibo Jiang Chen Zhou | 2011 | Acta Biochimica et Biophysica Sinica2011，43，3： | 6 |
| 4 | Retavperitoneal lap- aroscopic pyelolithotomy and ureterolithotomy and 34 cases of 显示文摘 | Xiong Lin Tan Wanlong Peng Hongmei | 2006 | Chinese minimally invasive surg2006，24，6： | 1 |
| 5 | A Novel Therapeutic Vaccine of Mouse GM-CSF Surface Modified MB49 Cells Against Metastatic Bladder Cancer显示文摘 | Xinji Zhang Xiaojun Shi Jinlong Li Zhiming Hu Dong Zhou Jimin Gao Wanlong Tan | 2012 | The Journal of Urology2012，，3： | 1 |
| 6 | Distributed Multi-Cell Multi-User MISO Downlink Beamforming via Deep Reinforcement Learning显示文摘The sum rate maximization beamforming problem for a multi-cell multi-user multiple-input single-output interference channel(MISO-IC)system is considered.Conventionally,the centralized and distributed beamforming solutions to the MISO-IC system have high computational complexity and bear a heavy burden of channel state information exchange between base stations(BSs),which becomes even much worse in a large-scale antenna system.To address this,we propose a distributed deep reinforcement learning(DRL)based approach with lim⁃ited information exchange.Specifically,the original beamforming problem is decomposed of the problems of beam direction design and power allocation and the costs of information exchange between BSs are significantly reduced.In particular,each BS is provided with an inde⁃pendent deep deterministic policy gradient network that can learn to choose the beam direction scheme and simultaneously allocate power to users.Simulation results illustrate that the proposed DRL-based approach has comparable sum rate performance with much less information exchange over the conventional distributed beamforming solutions. | JIA Haonan HE Zhenqing TAN Wanlong RUI Hua LIN Wei | 2022 | ZTE Communications2022，20，4： | 0 |
| 7 | Characterization of interfaces: Lessons from the past for the future of perovskite solar cells显示文摘A photovoltaic technology historically goes through two major steps to evolve into a mature technology. The first step involves advances in materials and is usually accompanied by the rapid improvement of power conversion efficiency. The second step focuses on interfaces and is usually accompanied by significant stability improvement. As an emerging generation of photovoltaic technology, perovskite solar cells are transitioning to the second step of their development when a significant focus shifts toward interface studies and engineering. While various interface engineering strategies have been developed, interfacial characterization is crucial to show the effectiveness of interfacial modification. Here, we review the characterization techniques that have been utilized in studying interface properties in perovskite solar cells. We first summarize the main roles of interfaces in perovskite solar cells, and then we discuss some typical characterization methodologies for morphological, optical,and electrical studies of interfaces. Successful experiences and existing problems are analyzed when discussing some commonly used methods. We then analyze the challenges and provide an outlook for further development of interfacial characterizations. This review aims to evoke strengthened research devotion on novel and persuasive interfacial engineering. | Wanlong Wang Dongyang Zhang Rong Liu Deepak Thrithamarassery Gangadharan Furui Tan Makhsud I.Saidaminov | 2022 | Journal of Semiconductors2022，43，5： | 0 |
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